SitesBLAST

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
26% identity, 87% coverage: 20:411/453 of query aligns to 93:481/582 of Q9JI12

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Q9JI12

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H128 (≠ F53) mutation to A: Greatly lowers L-glutamate transport.
R184 (= R113) mutation R->A,E,K: Greatly lowers L-glutamate transport.
E191 (= E120) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
R322 (≠ I254) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.

Sites not aligning to the query:

88 R→A: Impairs synaptic transmission. Abolishes the chloride ion conductance.

7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
26% identity, 87% coverage: 20:411/453 of query aligns to 35:423/452 of 7t3nA

query
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7t3nA

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binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y77 (= Y60), Y137 (≠ F124), Y165 (= Y152), R264 (≠ I254), F268 (≠ T258), Y269 (= Y259)
binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: E152 (= E139), G163 (≠ A150)

Sites not aligning to the query:

binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: 12, 13

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
25% identity, 90% coverage: 29:436/453 of query aligns to 88:489/495 of Q9NRA2

query
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Q9NRA2

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K136 (= K77) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ A128) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ AS 143:144) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
I--------L 266:267 (≠ IADNGGLVDM 210:219) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ DQDRG 220:224) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (= G281) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (= P287) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
G371 (= G322) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
26% identity, 90% coverage: 29:436/453 of query aligns to 88:489/495 of Q8BN82

query
sites
Q8BN82

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H183 (≠ A128) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
26% identity, 90% coverage: 29:436/453 of query aligns to 88:489/495 of Q5Q0U0

query
sites
Q5Q0U0

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K136 (= K77) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
R168 (= R113) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ V116) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (= G117) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E120) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ A121) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (= F124) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P125) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ A128) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ V131) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSLKN 268:272 (≠ DMDQD 218:222) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
P334 (= P287) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
G371 (= G322) mutation to V: Remains in the endoplasmic reticulum.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.

Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
26% identity, 80% coverage: 50:411/453 of query aligns to 111:464/497 of Q9Y2C5

query
sites
Q9Y2C5

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I

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A

P

P

K

R

Q

Y

I

-

A

T

G

G

L

F

S

L

G

K

G

G

L

L

F

L

N

Q

M

V

F

F

G

A

N

H

I

I

A

A

S

G

I

A

T

I

T

S

P

P

I

T

V

A

I

A

G

G

Y

F

I

F

I

I

S

S

T

Q

T

D

G

S

S

E

F

F

K

G

W

W

A

R

L

N

V

V

F

F

V

L

A372 (≠ I321) to T: in dbSNP:rs11754288

Sites not aligning to the query:

66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the tranport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the tranport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the tranport of thyroid hormones T3 and T4; when associated with A-75 and A-90.

Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
25% identity, 83% coverage: 34:410/453 of query aligns to 68:443/493 of Q03567

query
sites
Q03567

A

S

G
x
N

S

S

L

V

Q

I

K

G

D

E

L

F

G

D

I

W

D

D

A

K

V

Q

T

T

L

T

G

G

Y

M

I

V

F

L

S

S

A

S

F

F

G

F

W

Y

A

G

Y

Y

V

I

A

G

G

S

Q

Q

I

I

P

I

G

G

W

H

L

L

L

A

D

S

R

R

F

Y

G

G

S

G

K

K

K

R

V

V

Y

V

A

F

L

V

S

T

I

I

F

L

T

G

W

S

S

A

L

L

F

L

T

T

V

L

L

L

Q

N

G

-

Y

-

V

-

G

-

E

P

F

V

G

A

V

A

S

R

T

T

A

S

I

E

V

Y

A

A

L

L

F

A

V

I

L

L

R

R

F

A

L

A

V

I

G

G

L

F

A

L

E

Q

A

G

P

A

S

T

F

F

P

P

G

A

N

M

A

H

R

T

I

M

V

W

A

S

A

V

W

W

F

G

P

P

T

P

A

L

E

E

R

L

G

S

T

V

A

L

S

T

A

G

I

V

F

T

N

Y

S

A

A

G

Q

A

Y

Q

F

I

A

G

T

N

V

V

I

I

F

V

A

L

P

P

L

L

M

S

G

G

W

F

I

L

V

C

-

E

Y

Y

S

G

F

F

-

D

-

G

G

G

W

W

Q

P

H

S

V

I

F

F

M

Y

V

I

M

I

G

G

G

V

I

F

G

G

I

V

V

L

F

W

S

T

L

A

I

V

W

W

L

W

K

Y

I

V

-

S

I

S

H

D

S

K

P

P

R

A

Q

T

H

H

P

P

M

R

I

I

N

T

D

P

S

E

E

E

F

K

K

Q

H

Y

I

I

A

V

D

-

N

T

G

A

G

V

L

E

V

A

D

S

M

M

D

G

Q

K

D

D

R

T

G

G

K

K

R

V

S

P

D

S

G

T

P

P

K

-

W

W

D

-

Y

-

V

-

R

I

Q

K

L

I

L

L

S

T

N

S

R

P

M

A

M

V

L

W

G

A

V

C

Y

W

L

A

G

G

Q

H

Y

F

C

A

I

G

N

D

G

W

I

G

T

A

Y

Y

F

T

F

M

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L

T

V

W

S

F

L

P

P

V

S

Y

F

L

L

V

K

Q

D

E

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R

L

G

G

M

L

T

N

I

L

L

S

K

S

A

L

G

G

F

A

I

V

A

A

S

S

L

I

P

P

A

Y

I

I

C

A

G

Y

F

F

I

L

G

A

G

I

V

N

L

A

G

G

L

V

I

L

S

A

D

D

Y

T

L

L

L

R

R

S

K

K

G

G

-

I

-

L

H

S

S

T

L

L

T

N

F

T

A

R

R

R

K

A

A

A

P

M

I

L

I

V

G

A

G

L

L

I

L

G

L

Q

S

G

T

I

S

F

I

L

V

V

A

A

C

S

N

G

Y

Y

V

C

N

G

I

C

-

G

-

Q

E

D

W

V

I

L

V

V

V

I

G

I

F

F

M

I

A

T

L

C

A

G

F

M

F

A

G

I

K

S

G

G

V

L

G

Q

A

Y

L

A

G

G

W

F

A

V

V

V

V

N

S

Y

D

L

T

E

S

I

P

A

K

P

Q

P

I

F

A

S

G

G

L

T

S

V

G

M

G

G

L

T

F

G

N

N

M

T

F

I

G

S

N

A

I

L

A

A

S

G

I

I

T

I

T

S

P

P

I

A

V

V

I

S

G

S

Y

Y

I

L

I

T

S

P

T

N

T

G

G

T

S

Q

F

E

K

E

W

W

A

Q

L

M

V

V

F

L

N69 (≠ G35) modified: carbohydrate, N-linked (GlcNAc...) asparagine

Q9GQQ0 Protein spinster; Protein benchwarmer; Protein diphthong from Drosophila melanogaster (Fruit fly) (see paper)
26% identity, 79% coverage: 10:365/453 of query aligns to 113:448/605 of Q9GQQ0

query
sites
Q9GQQ0

R

Q

Y

W

L

F

I

T

L

V

L

T

M

V

L

L

F

C

L

F

V

V

T

N

T

L

I

I

N

N

Y

Y

A

M

D

D

R

R

A

F

T

T

I

I

A

A

I

G

A

V

G

L

S

T

S

D

L

V

Q

R

K

N

D

D

L

F

G

D

I

I

D

G

A

N

V

D

T

S

L

A

G

G

Y

L

I

L

F

Q

S

T

A

V

F

F

G

V

W

I

A

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Y

Y

V

M

A

V

G

C

Q

A

I

P

P

I

G

F

G

G

W

Y

L

L

L

G

D

D

R

R

F

Y

G

S

S

R

K

P

K

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V

I

Y

M

A

A

L

V

S

G

I

V

F

G

T

L

W

W

S

S

L

T

F

T

T

T

V

L

L

L

Q

G

G

S

Y

F

V

M

G

K

E

Q

F

F

G

G

V

-

S

-

T

-

A

-

I

-

V

-

A

W

L

F

F

I

V

A

L

F

R

R

F

A

L

L

V

V

G

G

L

I

A

G

E
|
E

A

A

P

S

S

Y

F

S

P

T

G

I

N

A

A

P

R

T

I

I

V

I

A

S

A

D

W

L

F

F

P

V

T

H

A

D

E

M

R

R

G

S

T

K

A

M

S

L

A

A

I

L

F

F

N

-

S

-

A

-

Q

-

Y

Y

F

F

A

A

T

I

V

P

I

V

F

G

A

S

P

G

L

L

M

-

G

G

W

Y

I

I

V

V

Y

G

S

S

-

K

-

T

-

A

-

H

-

L

-

A

F

N

G

D

W

W

Q

R

H

W

V

A

F

L

M

R

V

V

M

T

G

P

G

I

I

L

G

G

I

I

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V

F

A

S

V

L

F

I

L

W

I

L

L

K

L

I

I

I

K

H

D

S

P

P

V

R

R

Q

G

H

H

P

-

M

-

I

-

N

-

D

-

S

S

E

E

F

G

K

S

H

H

I

-

A

-

D

-

N

N

G

L

G

E

L

A

V

T

D

T

M

Y

D

K

Q

Q

D

D

R

-

G

-

K

-

R

-

S

-

D

-

G

-

P

-

K

-

W

-

D

-

Y

-

V

I

R

K

Q

A

L

L

L

V

S

R

N

N

R

R

M

S

M

F

L

M

G

L

V

S

Y

T

L

A

G

G

Q

F

Y

T

C

C

I

V

N

A

G

F

I

V

T

A

Y

G

F

A

F

L

L

A

T

W

W

W

F

G

P

P

V

S

Y

F

L

I

-

Y

-

L

-

G

-

M

-

K

V

M

Q

Q

E

P

R

G

G

N

M

E

T

N

I

I

L

V

K

Q

-

D

-

V

A

A

G

F

F

N

I

F

A

G

S

V

L

I

P

T

A

M

I

L

C

A

G

G

F

L

I

L

G

G

G

V

V

P

L

L

G

G

G

S

L

F

I

L

S

S

D

Q

Y

Y

L

L

L

V

R

K

K

R

G

-

H

-

S

-

L

-

T

-

F

Y

A

P

R

T

K

A

A

D

P

P

I

V

I

I

G

C

-

A

-

F

G

G

L

L

L

V

S

S

T

A

S

P

I

L

V

L

-

T

-

G

A

A

C

C

N

L

Y

L

V

V

N

N

I

S

E

N

W

-

I

-

V

S

V

V

G

G

F

T

M

Y

A

A

L

L

A

I

F

F

G

G

K

Q

G

L

V

A

G

L

A

N

L

L

G

N

W

W

A

A

V

I

V

V

S

A

D

D

E217 (= E120) mutation to K: In bnch(N); leads to storage in yolk spheres during oogenesis and results in widespread accumulation of enlarged lysosomal and late endosomal inclusions.

Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
28% identity, 30% coverage: 7:140/453 of query aligns to 24:149/448 of Q51955

query
sites
Q51955

T

S

H

R

V

Y

R

Q

Y

W

L

R

I

V

L

V

L

L

M

L

L

C

F

F

L

L

V

I

T

V

T

F

I

L

N
x
D

Y

G

A

L

D
|
D

R

T

A

A

T

A

I

M

A

G

I

F

A

I

G

A

S

P

S

A

L

L

Q

S

K

Q

D

E

L

W

G

G

I

I

D

D

A

R

V

A

T

S

L

L

G

G

Y

P

I

V

F

M

S

S

A

A

F

A

G

L

W

I

A

G

Y

M

V

V

A

F

G

G

Q

A

I

L

P

G

G

S

G
|
G

W

P

L

L

L

A

D
|
D

R

R

F

F

G
|
G

S

R

K

K

K

G

V

V

Y

L

A

V

L

G

S

A

I

V

F

L

T

V

W

F

S

G

L

G

F

F

T

S

V

L

L

A

Q

S

G

A

Y

Y

V

-

G

-

E

-

F

-

G

-

V

-

S

A

T

T

A

N

I

V

V

D

A

Q

L

L

F

L

V

V

L

L

R
|
R

F

F

L

L

V

T

G

G

L

L

A

G

E

L

A

G

P

A

S

G

F

M

P

P

G

N

N

A

A

T

R

T

I

L

V

L

A

S

A
x
E

W

Y

F

T

P

P

T

-

A

-

E

E

R

R

D41 (≠ N24) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
D44 (= D27) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
G85 (= G68) mutation to V: Abolishes 4-HBA transport and chemotaxis.
D89 (= D72) mutation to N: Abolishes 4-HBA transport and chemotaxis.
G92 (= G75) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
R124 (= R113) mutation to A: Abolishes 4-HBA transport.
E144 (≠ A133) mutation to A: Strong decrease in 4-HBA transport.

Sites not aligning to the query:

183 H→A: Decrease in 4-HBA transport and chemotaxis.
323 D→N: Abolishes 4-HBA transport and chemotaxis.
328 H→A: Decrease in 4-HBA transport and chemotaxis.; H→R: Decrease in 4-HBA transport and loss of chemotaxis.
386 R→A: Strong decrease in 4-HBA transport.
398 R→A: Abolishes 4-HBA transport.
444 H→A: No change in 4-HBA transport and chemotaxis.

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
24% identity, 77% coverage: 10:359/453 of query aligns to 20:359/452 of Q5EXK5

query
sites
Q5EXK5

R

Q

Y

W

L

R

I

V

L

I

M

C

L

C

F

F

L

L

V

V

T

V

T

M

I

L

N

D

Y

G

A

F

D

D

R

T

A

A

T

A

I

I

A

G

I

F

A

I

G

A

S

P

S

D

L

I

Q

R

K

S

D

H

L

W

G

Q

I

L

D

T

A

A

V

G

T

D

L

L

G

A

Y

P

I

L

F

F

S

G

A

A

F

G

G

L

W

L

A

G

Y

L

V

T

A

A

G

G

Q

A

I

L

P

L

G

C

G

G

W

P

L

L

L

S

D
|
D

R

R

F

F

G

G

S

R

K

K