SitesBLAST

1:57 Interaction with SYT1
529:566 (Microbial infection) C.botulinum neurotoxin type A-binding
559 N→A: Loss of one glycosylation site. No effect on C.botulinum neurotoxin type A (BoNT/A, botA) binding, but reduces the uptake of BoNT/A.

Q496J9 Synaptic vesicle glycoprotein 2C from Homo sapiens (Human) (see 4 papers)
28% identity, 33% coverage: 70:217/446 of query aligns to 203:347/727 of Q496J9

query
sites
Q496J9

L

V

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G

A

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Sites not aligning to the query:

519:563 (Microbial infection) C.botulinum neurotoxin type A-binding
534 modified: carbohydrate, N-linked (GlcNAc...) asparagine
559 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: No change in interaction with C.botulinum neurotoxin type A heavy chain (botA, BoNT/A HC). Decreased molecular weight probably due to glycosylation loss, decreased interaction with BoNT/A HC.; N→Q: Decreased molecular weight probably due to glycosylation loss, decreased binding to BoNT/A HC. Greater reduction in weight; when associated with Q-565.
561 S→A: Decreased molecular weight probably due to glycosylation loss, decreased binding to BoNT/A HC.
563 F→A: No longer interacts with BoNT/A HC.
565 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→Q: Decreased molecular weight probably due to glycosylation loss, no change in binding to BoNT/A heavy chain. Greater reduction in weight; when associated with Q-559.

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
24% identity, 46% coverage: 28:233/446 of query aligns to 56:245/444 of Q8NLB7

query
sites
Q8NLB7

Y

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D57 (= D29) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (≠ K81) mutation to A: Loss of transport activity.

Sites not aligning to the query:

54 D→A: Loss of transport activity.; D→E: Retains 50% of its transport activity.
309 W→V: Loss of transport activity.
312 D→A: Loss of transport activity.
313 R→A: Loss of transport activity.
317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

A0A0H2VG78 Glucose transporter GlcP; Glucose/H(+) symporter from Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) (see paper)
27% identity, 35% coverage: 76:231/446 of query aligns to 63:202/446 of A0A0H2VG78

query
sites
A0A0H2VG78

G

G

P

P

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D

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R102 (= R123) mutation to A: Loss of transport activity.
I105 (≠ Q126) mutation to S: Affects symport activity. May function as an uniporter.
E122 (= E143) mutation to A: Loss of transport activity.
Q137 (≠ M158) mutation to A: Loss of transport activity.

Sites not aligning to the query:

22 D→N: Affects symport activity. May function as an uniporter.
250 Q→A: Loss of transport activity.
251 Q→A: Loss of transport activity.
256 N→A: Loss of transport activity.
357 W→A: Loss of transport activity.

Query Sequence

>AO353_05775 FitnessBrowser__pseudo3_N2E3:AO353_05775
VGIQQKNPARQRRRAFIGATSGHLIEWYDYGVYGFLAVYIGKAFFVSDDPTSSLLASFAA
FALSFFIRPLGGLFFGPLADKIGRRKTLITVLVMMAGSTVLLGLLPTYATLGIAAPILLV
LIRCVQGFSAGGEIGTITSFISEYAGPGRRGFATCWLMVTAVLGLLLGGAVANGMTWVMG
ADLMQAWGWRIPFLIAAPLGLISMYIRLKLEDSPEFLALQRAGETSKAPLREVWQWKRAI
ALVFFIITLHSSIFYLVLTFASTYMSSILKFDSGTTLLYVFVASLSAAVVMPFGGAFTDK
YGRKPFLLVVGTLATLAMYWLFKSAPTATPASFIYPLMTVAILFGLYASSTYALMSELLP
TRIRSTGIAVAYNIPVAVFGGSAPLISTWLIKVTGDITSPWYFYIGTGVVSLIALVLLRK
EDFVACASPAEKPNVGRADLALAPTP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory