SitesBLAST
Comparing AO353_05960 FitnessBrowser__pseudo3_N2E3:AO353_05960 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1krhA X-ray structure of benzoate dioxygenase reductase (see paper)
40% identity, 99% coverage: 2:336/340 of query aligns to 2:335/337 of 1krhA
- active site: C306 (= C307)
- binding flavin-adenine dinucleotide: Y143 (= Y142), R155 (= R154), S156 (= S155), Y157 (= Y156), S158 (= S157), V171 (≠ L171), V172 (≠ I172), G178 (= G178), K179 (≠ V179), M180 (= M180), S181 (= S181), T219 (= T219), E332 (= E333), F334 (= F335), S335 (≠ T336)
- binding fe2/s2 (inorganic) cluster: M38 (≠ L38), D39 (= D39), C40 (= C40), R41 (= R41), G43 (= G43), C45 (= C45), G46 (= G46), C48 (= C48), C82 (= C80)
Sites not aligning to the query:
P0DPQ8 Aromatic O-demethylase, reductase subunit; NADH--hemoprotein reductase; EC 1.6.2.- from Amycolatopsis sp. (strain ATCC 39116 / 75iv2) (see paper)
32% identity, 94% coverage: 20:339/340 of query aligns to 15:334/334 of P0DPQ8
- C35 (= C40) binding [2Fe-2S] cluster
- C40 (= C45) binding [2Fe-2S] cluster
- C43 (= C48) binding [2Fe-2S] cluster
- C75 (= C80) binding [2Fe-2S] cluster
- RQYS 145:148 (≠ RSYS 154:157) binding FAD
- HVR 162:164 (≠ LIR 171:173) binding FAD
- VAT 170:172 (≠ VMS 179:181) binding FAD
- T215 (= T219) binding FAD
- F330 (= F335) binding FAD
- S334 (≠ N339) binding FAD
5ogxA Crystal structure of amycolatopsis cytochrome p450 reductase gcob. (see paper)
32% identity, 94% coverage: 20:339/340 of query aligns to 14:333/333 of 5ogxA
- binding flavin-adenine dinucleotide: Y132 (= Y142), R144 (= R154), Q145 (≠ S155), Y146 (= Y156), S147 (= S157), H161 (≠ L171), V162 (≠ I172), V165 (≠ L175), G168 (= G178), V169 (= V179), A170 (≠ M180), T171 (≠ S181), T214 (= T219), F329 (= F335), P331 (≠ D337), S333 (≠ N339)
- binding fe2/s2 (inorganic) cluster: C34 (= C40), N35 (≠ R41), G37 (= G43), C39 (= C45), G40 (= G46), C42 (= C48), C74 (= C80)
1tvcA Fad and nadh binding domain of methane monooxygenase reductase from methylococcus capsulatus (bath) (see paper)
33% identity, 60% coverage: 135:338/340 of query aligns to 42:247/250 of 1tvcA
- active site: Y63 (= Y156), S64 (= S157), L215 (≠ V306)
- binding dihydroflavine-adenine dinucleotide: F49 (≠ Y142), R61 (= R154), S62 (= S155), Y63 (= Y156), S64 (= S157), L78 (= L171), I79 (= I172), R80 (= R173), L82 (= L175), F87 (≠ M180), G128 (= G220), N155 (≠ R247), E159 (≠ D251), S186 (= S278), G187 (≠ P279), E242 (= E333), F244 (= F335), L245 (≠ T336)
Sites not aligning to the query:
Q03304 Toluene-4-monooxygenase system, ferredoxin--NAD(+) reductase component; T4MO; Ferredoxin--NAD(+) reductase; Toluene-4-monooxygenase systme, electron transfer component; EC 1.18.1.3 from Ectopseudomonas mendocina (Pseudomonas mendocina) (see paper)
28% identity, 89% coverage: 17:320/340 of query aligns to 13:309/326 of Q03304
- C36 (= C40) binding [2Fe-2S] cluster
- C41 (= C45) binding [2Fe-2S] cluster
- C44 (= C48) binding [2Fe-2S] cluster
- C76 (= C80) binding [2Fe-2S] cluster
- RAYS 146:149 (≠ RSYS 154:157) binding FAD
- IVK 162:164 (≠ LIR 171:173) binding FAD
- KVS 170:172 (≠ VMS 179:181) binding FAD
4wqmA Structure of the toluene 4-monooxygenase nadh oxidoreductase t4mof, k270s k271s variant (see paper)
27% identity, 89% coverage: 17:320/340 of query aligns to 13:309/326 of 4wqmA
- binding flavin-adenine dinucleotide: C36 (= C40), S38 (≠ E42), W57 (≠ Y61), Y135 (= Y142), R146 (= R154), A147 (≠ S155), Y148 (= Y156), S149 (= S157), I162 (≠ L171), V163 (≠ I172), G169 (= G178), K170 (≠ V179), V171 (≠ M180), S172 (= S181), S209 (≠ T219)
- binding fe2/s2 (inorganic) cluster: Y34 (≠ L38), C36 (= C40), N37 (≠ R41), G39 (= G43), C41 (= C45), G42 (= G46), C44 (= C48), C76 (= C80)
Sites not aligning to the query:
7c3bC Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
27% identity, 93% coverage: 21:335/340 of query aligns to 21:333/334 of 7c3bC
7c3aA Ferredoxin reductase in carbazole 1,9a-dioxygenase (see paper)
26% identity, 93% coverage: 21:335/340 of query aligns to 20:320/321 of 7c3aA
- binding flavin-adenine dinucleotide: C39 (= C40), S41 (≠ E42), W60 (≠ Y61), R152 (= R154), A153 (≠ S155), Y154 (= Y156), S155 (= S157), Y169 (≠ L171), I170 (= I172), G176 (= G178), R177 (≠ V179), F178 (≠ M180), S179 (= S181), A218 (≠ T219), F320 (= F335)
- binding fe2/s2 (inorganic) cluster: Y37 (≠ L38), C39 (= C40), A40 (≠ R41), G42 (= G43), C44 (= C45), G45 (= G46), C47 (= C48), C80 (= C80)
Sites not aligning to the query:
7c3bB Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
26% identity, 93% coverage: 21:335/340 of query aligns to 20:305/306 of 7c3bB
7qu0A X-ray structure of fad domain of nqrf of klebsiella pneumoniae (see paper)
26% identity, 54% coverage: 154:335/340 of query aligns to 83:279/279 of 7qu0A
- binding ~{N}-[2,6-bis(fluoranyl)phenyl]ethanamide: S213 (= S275), F227 (≠ Y287), M256 (= M312)
- binding flavin-adenine dinucleotide: R83 (= R154), A84 (≠ S155), Y85 (= Y156), S86 (= S157), A104 (vs. gap), T105 (vs. gap), P106 (vs. gap), P107 (vs. gap), A113 (≠ L175), P114 (= P176), P115 (≠ D177), G116 (= G178), I117 (≠ V179), M118 (= M180), S119 (= S181), F279 (= F335)
Sites not aligning to the query:
7qtyA X-ray structure of fad domain of nqrf of klebsiella pneumoniae (see paper)
26% identity, 54% coverage: 154:335/340 of query aligns to 83:279/279 of 7qtyA
- binding 1-(furan-2-ylmethyl)-3-(2-methylphenyl)thiourea: A184 (≠ V246), R185 (= R247), S213 (= S275), F227 (≠ Y287), H229 (≠ A289), V255 (≠ P311), M256 (= M312)
- binding flavin-adenine dinucleotide: R83 (= R154), A84 (≠ S155), Y85 (= Y156), S86 (= S157), N100 (≠ L171), A104 (vs. gap), T105 (vs. gap), P107 (vs. gap), A113 (≠ L175), P114 (= P176), G116 (= G178), I117 (≠ V179), M118 (= M180), S119 (= S181), F279 (= F335)
Sites not aligning to the query:
7qu5A X-ray structure of fad domain of nqrf of pseudomonas aeruginosa (see paper)
25% identity, 54% coverage: 154:335/340 of query aligns to 83:278/280 of 7qu5A
- binding flavin-adenine dinucleotide: R83 (= R154), A84 (≠ S155), Y85 (= Y156), S86 (= S157), N100 (≠ L171), A104 (vs. gap), L112 (= L175), P113 (= P176), G115 (= G178), Q116 (≠ V179), M117 (= M180), S118 (= S181), A155 (≠ T219), F278 (= F335)
- binding gamma-Valerolactone: S105 (vs. gap), P108 (vs. gap), D196 (≠ S259), W207 (≠ Y270), H208 (≠ T271), L209 (≠ P272)
Sites not aligning to the query:
7qu3A X-ray structure of fad domain of nqrf of pseudomonas aeruginosa (see paper)
25% identity, 54% coverage: 154:335/340 of query aligns to 83:278/279 of 7qu3A
- binding flavin-adenine dinucleotide: R83 (= R154), A84 (≠ S155), Y85 (= Y156), S86 (= S157), N100 (≠ L171), A104 (vs. gap), S105 (vs. gap), L112 (= L175), P113 (= P176), G115 (= G178), Q116 (≠ V179), M117 (= M180), S118 (= S181), A155 (≠ T219), F278 (= F335)
- binding 4-(benzimidazol-1-ylmethyl)benzenecarbonitrile: R102 (= R173), G154 (= G218), G182 (= G245), F226 (≠ Y287), I227 (= I288), P252 (= P309), M255 (= M312)
Sites not aligning to the query:
2r6hC Crystal structure of the domain comprising the NAD binding and the fad binding regions of the nadh:ubiquinone oxidoreductase, na translocating, f subunit from porphyromonas gingivalis
25% identity, 54% coverage: 154:335/340 of query aligns to 86:288/289 of 2r6hC
- active site: C260 (= C307)
- binding flavin-adenine dinucleotide: R86 (= R154), A87 (≠ S155), Y88 (= Y156), S89 (= S157), N103 (≠ L171), A107 (vs. gap), T108 (vs. gap), I122 (≠ L175), K123 (≠ P176), P124 (≠ D177), G125 (= G178), I126 (≠ V179), S127 (≠ M180), S128 (= S181), F288 (= F335)
Sites not aligning to the query:
6l2uA Soluble methane monooxygenase reductase fad-binding domain from methylosinus sporium. (see paper)
49% identity, 22% coverage: 129:203/340 of query aligns to 30:103/103 of 6l2uA
- binding flavin-adenine dinucleotide: F43 (≠ Y142), R55 (= R154), S56 (= S155), Y57 (= Y156), S58 (= S157), F71 (≠ L171), F71 (≠ L171), I72 (= I172), R73 (= R173), L75 (= L175), P76 (= P176), P76 (= P176), G78 (= G178), A79 (≠ V179), F80 (≠ M180), S81 (= S181)
8a1uF Sodium pumping nadh-quinone oxidoreductase with substrates nadh and q2 (see paper)
24% identity, 54% coverage: 154:335/340 of query aligns to 209:405/405 of 8a1uF
- binding flavin-adenine dinucleotide: R209 (= R154), A210 (≠ S155), Y211 (= Y156), S212 (= S157), A230 (vs. gap), P233 (vs. gap), V239 (≠ L175), P240 (= P176), G242 (= G178), Q243 (≠ V179), M244 (= M180), A282 (≠ T219), F405 (= F335)
- binding 1,4-dihydronicotinamide adenine dinucleotide: S212 (= S157), R228 (= R173), G281 (= G218), A282 (≠ T219), G309 (= G245), R311 (= R247), S339 (= S275), F353 (≠ Y287), C377 (= C307), G378 (= G308), P379 (= P309), M381 (≠ P311), M382 (= M312), D403 (≠ E333)
Sites not aligning to the query:
8a1yF Sodium pumping nadh-quinone oxidoreductase with inhibitor hqno (see paper)
24% identity, 54% coverage: 154:335/340 of query aligns to 210:406/408 of 8a1yF
- binding flavin-adenine dinucleotide: R210 (= R154), A211 (≠ S155), Y212 (= Y156), S213 (= S157), V228 (≠ I172), R229 (= R173), A231 (vs. gap), V240 (≠ L175), P241 (= P176), G243 (= G178), Q244 (≠ V179), M245 (= M180)
Sites not aligning to the query:
A5F5Y4 Na(+)-translocating NADH-quinone reductase subunit F; Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F; EC 7.2.1.1 from Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) (see paper)
24% identity, 54% coverage: 154:335/340 of query aligns to 210:406/408 of A5F5Y4
- R210 (= R154) mutation to L: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
- Y212 (= Y156) mutation to L: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
- S246 (= S181) mutation to A: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
Sites not aligning to the query:
- 70 binding [2Fe-2S] cluster; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
- 76 binding [2Fe-2S] cluster; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
- 79 binding [2Fe-2S] cluster; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
- 111 binding [2Fe-2S] cluster; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
4u9uA Crystal structure of nqrf fad-binding domain from vibrio cholerae (see paper)
24% identity, 54% coverage: 154:335/340 of query aligns to 81:277/279 of 4u9uA
- binding flavin-adenine dinucleotide: R81 (= R154), A82 (≠ S155), Y83 (= Y156), S84 (= S157), N98 (≠ L171), A102 (≠ L175), T103 (≠ P176), P104 (≠ D177), V111 (vs. gap), P112 (vs. gap), G114 (= G178), Q115 (≠ V179), M116 (= M180), S117 (= S181), F277 (= F335)
- binding magnesium ion: R186 (≠ A250), M188 (≠ L252)
- binding sodium ion: A198 (= A262), N201 (≠ I265), D202 (≠ P266), F204 (= F268)
Sites not aligning to the query:
3ozwA The crystal structure of flavohemoglobin from r. Eutrophus in complex with ketoconazole (see paper)
26% identity, 59% coverage: 135:335/340 of query aligns to 183:396/403 of 3ozwA
- binding flavin-adenine dinucleotide: R206 (= R154), Q207 (≠ S155), Y208 (= Y156), S209 (= S157), S222 (≠ F170), V223 (≠ L171), K224 (≠ I172), E226 (≠ L174), P232 (= P176), G234 (= G178), Y235 (≠ V179), V236 (≠ M180), S237 (= S181), V276 (≠ T219), T279 (≠ S222), V395 (≠ K334), F396 (= F335)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 46, 50
- binding protoporphyrin ix containing fe: 42, 43, 81, 85, 88, 90, 94, 95, 98, 126, 130, 133
- binding 1-acetyl-4-(4-{[(2R,4S)-2-(2,4-dichlorophenyl)-2-(1H-imidazol-1-ylmethyl)-1,3-dioxolan-4-yl]methoxy}phenyl)piperazine: 53, 56, 102, 398
Query Sequence
>AO353_05960 FitnessBrowser__pseudo3_N2E3:AO353_05960
MNHKVAFSFADGKTLFFPVQANEILLDAALRNGINIPLDCREGVCGTCQGRCESGDYSQD
YVDEEALSSLDLQQRKMLTCQTRVKSDAAFYFDFASSLCNAAGPEQLSGTVTHVRQVSTS
TAILHLDLGAATQPLDFLPGQYARLLIPGTMSKRSYSFANRPSSSNQLQFLIRLLPDGVM
SNYIRERCQVGDEIALEAPLGAFYLRHIARPLILVAGGTGLSALLGMLDEIVARGCEQPV
HLYYGVRDAADLCEGERFSAYAHRIPGFRYTPVVSDPSPGWEGKRGYIAEHFDACELRDA
AVDMYVCGPPPMVESIKRWLQDQTLENVQLYYEKFTDSNV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory