SitesBLAST

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
24% identity, 54% coverage: 83:323/450 of query aligns to 132:378/582 of Q9JI12

query
sites
Q9JI12

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R184 (= R136) mutation R->A,E,K: Greatly lowers L-glutamate transport.
E191 (= E143) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
R322 (≠ T265) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.

Sites not aligning to the query:

88 R→A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
128 H→A: Greatly lowers L-glutamate transport.

7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
22% identity, 56% coverage: 70:323/450 of query aligns to 58:320/452 of 7t3nA

query
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7t3nA

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binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y77 (= Y89), Y137 (= Y147), Y165 (≠ N175), R264 (≠ T265), F268 (= F269), Y269 (≠ W270)
binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: E152 (= E162), G163 (= G173)

Sites not aligning to the query:

binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: 12, 13

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
24% identity, 93% coverage: 27:444/450 of query aligns to 8:436/452 of Q5EXK5

query
sites
Q5EXK5

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D82 (= D101) mutation to A: Loss of activity.
V311 (≠ F317) mutation to W: Loss of activity.
D314 (≠ G320) mutation to A: Loss of activity.

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
24% identity, 60% coverage: 58:329/450 of query aligns to 88:355/495 of Q9NRA2

query
sites
Q9NRA2

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-

V

-

G

-

G

G

G

S

I

W

L

L

S

C

S

M

F

I

L

L

I

S

G

G

R

Q

G

A

V

A

N

D

P

N

I

L

K

R

A

A

R

K

K136 (= K106) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ V151) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ AT 166:167) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
IL 266:267 (≠ IQ 233:234) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ NEAEP 235:239) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (= G294) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (≠ I300) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794
371 G → V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
26% identity, 53% coverage: 58:294/450 of query aligns to 88:328/495 of Q8BN82

query
sites
Q8BN82

A

A

A

P

L

I

G

K

V

V

M

H

Q

H

P

N

I

H

L

T

A

G

K

K

E

K

M

Y

S

K

W

W

T

D

A

A

M

E

D

T

Y

Q

A

G

N

W

I

I

N

L

F

G

W

S

F

F

Q

F

V

Y

G

G

Y

Y

A

I

V

V

G

T

F

Q

V

I

L

P

Q

G

G

G

R

Y

L

I

I

A

D

S

R

R

I

V

G

G

V

G

K

K

R

L

V

L

F

L

F

G

C

L

A

G

V

I

L

L

L

G

W

T

S

S

L

V

A

F

T

T

G

L

A

F

H

T

G

P

L

L

A

A

T

A

S

D

-

L

-

G

A

V

L

V

G

T

F

L

M

V

V

V

C

L

R

R

F

A

I

L

L

E

G

G

L

L

T

G

E

E

A

G

A

V

N

T

Y

F

P

P

A

A

C

M

V
x
H

K

A

T

M

T

W

R

S

L

S

W

W

F

A

P

P

A

P

G

L

E

E

R

R

A

S

V

K

A

L

T

L

G

T

I

I

F

S

N

Y

A

A

G

G

T

A

N

Q

V

L

G

G

A

T

M

V

F

I

T

S

P

L

M

P

L

L

L

S

P

G

L

I

I

I

L

C

H

Y

V

Y

W

M

G

N

W

W

Q

T

A

Y

A

V

F

F

L

Y

C

L

M

F

S

G

A

I

L

V

G

G

G

I

I

V

W

W

L

F

V

I

F

L

W

W

G

M

-

W

L

I

K

V

Y

S

F

D

N

T

P

P

E

E

D

T

H

H

P

K

S

T

V

I

K

S

Q

H

S

Y

E

E

L

K

D

E

Y

Y

I

I

Q

V

N

S

E

S

A

L

E

K

P

N

E

Q

-

L

-

S

Q

Q

A

K

R

V

V

V

P

P

F

W

S

G

R

S

I

I

L

L

R

K

M

S

R

L

G

P

T

L

W

W

A

A

F

I

A

V

L

V

A

A

Y

H

S

F

M

S

T

Y

A

N

P

W

V

S

F

F

W

Y

F

T

Y

L

L

L

Y

T

W

L

L

L

P

P

P

T

F

Y

L

M

N

K

Q

E

Q

I

Y

-

N

-

L

L

G

R

I

F

N

N

V

V

T

Q

Q

E

M

N

G

G

H183 (≠ V151) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
26% identity, 37% coverage: 130:294/450 of query aligns to 162:328/495 of Q5Q0U0

query
sites
Q5Q0U0

L

V

G

A

F

L

M

I

V

V

C

L

R
|
R

F

A

I

L

L
x
E

G
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G

L

L

T

G

E
|
E

A
x
G

A

V

N

T

Y
x
F

P
|
P

A

A

C

M

V
x
H

K

A

T

M

T
x
W

R

S

L

S

W

W

F

A

P

P

A

P

G

L

E

E

R

R

A

S

V

K

A

L

T

L

G

T

I

I

F

S

N

Y

A

A

G

G

T

A

N

Q

V

L

G

G

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V

F

I

T

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P

L

M

P

L

L

L

S

P

G

L

I

I

I

L

C

H

Y

V

Y

W

M

G

N

W

W

Q

T

A

Y

A

V

F

F

L

Y

C

L

M

F

S

G

A

L

L

V

G

G

G

I

I

V

W

W

L

F

V

I

F

L

W

W

G

M

-

W

L

I

K

V

Y

S

F

D

N

T

P

P

E

E

D

T

H

H

P

K

S

T

V

I

K

S

Q

H

S

Y

E

E

L

K

D

E

Y

Y

I

I

Q

V

N
x
S

E
x
S

A
x
L

E
x
K

P
x
N

E

Q

-

L

-

S

Q

Q

A

K

R

V

V

V

P

P

F

W

S

V

R

S

I

I

L

L

R

K

M

S

R

L

G

P

T

L

W

W

A

A

F

I

A

V

L

V

A

A

Y

H

S

F

M

S

T

Y

A

N

P

W

V

T

F

F

W

Y

F

T

Y

L

L

L

Y

T

W

L

L

L

P

P

P

T

F

Y

L

M

N

K

Q

E

Q

I

Y

-

N

-

L

L

G

R

I

F

N

N

V

V

T

Q

Q

E

M

N

G

G

R168 (= R136) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ L139) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (= G140) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E143) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ A144) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (≠ Y147) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P148) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ V151) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ T154) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSLKN 268:272 (≠ NEAEP 235:239) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
136 K→E: Markedly decreases H(+)-coupled sialic acid transporter activity.
334 P→R: Abolishes H(+)-coupled sialic acid transporter activity.
371 G→V: Remains in the endoplasmic reticulum.

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
24% identity, 79% coverage: 56:412/450 of query aligns to 57:400/444 of Q8NLB7

query
sites
Q8NLB7

D
|
D

R

L

A

I

A

V

L

Y

G

G

V

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M

V

Q

Q

P

S

I

A

L

L

A

A

K

K

E

E

M

W

S

N

W

L

T

S

A

S

M

A

D

T

Y

L

A

G

N

T

I

I

N

G

F

S

W

T

F

A

Q

F

V

F

G

G

Y

M

A

A

V

I

G

G

F

A

V

V

L

F

Q

I

G

G

R

R

L

L

I

S

D

D

R
|
R

I

V

G

G

V

R

K

K

R

A

V

A

F

V

F

I

C

G

A

S

V

V

L

L

L

I

W

L

S

S

L

V

A

F

T

T

G

M

A

L

H

C

G

A

L

F

A

A

T

P

S

N

A

P

L

W

G

V

F

F

M

G

V

A

C

F

R

R

F

F

I

I

L

A

G

G

L

L

T

G

E

L

A

G

N

L

Y

V

P

P

A

S

C

V

V

N

K

A

T

M

T

T

R

S

L

D

W

L

F

V

P

P

A

R

G

K

E

T

R

M

A

S

V

A

A

W

T

A

G

T

I

V

F

M

N

M

A

S

G

G

T

V

N

P

V

I

G

G

A

G

M

S

F

I

T

A

P

A

M

V

L

L

L

A

P

L

L

V

I

V

L

V

-

P

-

S

H

E

V

E

W

W

G

G

W

W

Q

R

A

-

F

F

L

M

C

F

M

L

S

I

A

A

L

L

G

-

I

I

W

P

L

L

V

V

F

V

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-

G

G

L

L

K

P

Y

-

F

I

N

A

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M

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V

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P

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S

V

D

K

K

Q

A

S

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K

L

A

D

D

Y

H

I

D

Q

I

N

R

E

E

A

G

E

H

P

D

E

E

Q

P

A

A

R

G

V

-

P

-

F

F

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K

R

D

I

L

L

L

R

V

M

D

R

R

G

Y

T

R

W

W

A

I

-

S

-

I

-

W

F

F

A

A

L

L

A

A

Y

T

S

F

M

V

T

T

A

L

P

L

V

A

F

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W

Y

F

G

Y

L

L

G

Y

T

W

W

L

L

P

P

P

R

F

L

L

M

N

E

Q

T

Q

A

-

G

Y

Y

N

E

L

F

G

G

I

-

N

-

V

-

T

-

Q

-

M

-

G

-

I

-

P

H

L

A

I

L

I

M

I

F

Y

T

M

L

T

A

A

L

D

N

F

L

G

G

S

A

V

V

G

I

G

G

G

S

I

V

L

V

S

T

S

A

F
x
W

L

-

I

A

G

G

R
x
D

G
x
R

V

F

N

G

P

P

I
|
I

K

R

A

S

R

G

L

V

M

I

S

A

M

A

L

G

L

I

F

A

A

G

C

I

C

A

I

L

G

L

V

L

I

L

M

T

A

Y

A

P

G

P

S

V

S

T

N

A

L

V

W

Y

V

V

A

I

V

L

F

-

A

-

I

I

S

L

L

A

A

G

I

V

G

G

A

T

H

H

Q

G

A

T

W

Q

T

I

A

L

N

I

I

I

W

-

S

A

L

A

V

V

M

A

D

N

Y

F

T

Y

P

P

K

S

H

N

M

L

M

R

S

G

T

T

V

A

F

L

G

G

F

W

G

A

G

L

M

G

C

V

A

G

A
x
R

I

I

G

G

G

A

M

V

F

V

M

A

T

P

Q

Q

L

L

V

A

G

G

H

L

I

L

L

L

D57 (= D56) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (= R102) mutation to A: Loss of transport activity.
W309 (≠ F317) mutation to V: Loss of transport activity.
D312 (≠ R321) mutation to A: Loss of transport activity.
R313 (≠ G322) mutation to A: Loss of transport activity.
I317 (= I326) mutation I->H,Y: Loss of transport activity.
R386 (≠ A398) mutation to A: Loss of transport activity.

Sites not aligning to the query:

54 D→A: Loss of transport activity.; D→E: Retains 50% of its transport activity.

Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
23% identity, 37% coverage: 157:323/450 of query aligns to 191:360/497 of Q9Y2C5

query
sites
Q9Y2C5

W

W

F

A

P

P

A

P

G

L

E

E

R

R

A

S

V

Q

A

L

T

T

G

T

I

I

F

A

N

G

A

S

G

G

T

S

N

M

V

L

G

G

A

S

M

F

F

I

T

V

P

L

M

L

L

A

L

G

P

G

L

L

I

L

L

C

H

Q

V

T

W

I

G

G

W

W

Q

P

A

Y

A

V

F

F

L

Y

C

I

M

F

S

G

A

G

L

I

G

G

G

C

I

A

W

C

L

C

V

P

F

L

W

W

-

F

G

P

L

L

K

I

Y

Y

F

D

N

D

P

P

E

V

D

N

H

H

P

P

S

F

V

I

K

S

Q

A

S

G

E

E

L

K

D

R

Y

Y

I

I

-

V

-

C

-

S

-

L

-

A

Q

Q

N

Q

E

D

A

C

E

S

P

P

E

G

Q

W

A

S

R

-

V

L

P

P

F

I

S

R

R

A

I

M

L

I

R

K

M

S

R

L

G

P

T

L

W

W

A

A

F

I

A

L

L

V

A

S

Y

Y

S

F

M

C

T

E

A

Y

P

W

V

L

F

F

W

Y

F

T

Y

I

L

M

Y

A

W

Y

L

T

P

P

P

T

F

Y

L

I

N

S

Q

S

Q

V

Y

-

N

-

L

L

G

Q

I

A

N

N

V

L

T

R

Q

D

M

S

G

G

I

I

P

L

L

S

I

A

I

L

I

P

Y

F

M

V

T

V

A

G

D

C

F

I

G

C

S

I

V

I

G

L

G

G

I

L

L

L

S

A

S

D

F

F

L

L

I

L

G

S

R

R

G

K

V

I

Sites not aligning to the query:

66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the tranport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the tranport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the tranport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
372 A → T: in dbSNP:rs11754288

Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
24% identity, 36% coverage: 37:196/450 of query aligns to 25:184/448 of Q51955

query
sites
Q51955

K

R

T

Y

R

Q

W

W

G

R

M

V

L

V

A

L

L

L

V

C

F

F

F

L

A

I

T

V

T

F

L

L

N
x
D

Y

G

I

L

D
|
D

R

T

A

A

L

M

G

G

V

F

M

I

Q

A

P

P

I

A

L

L

A

S

K

Q

E

E

M

W

S

G

W

I

T

D

A

R

M

A

D

S

Y

L

A

G

N

P

I

V

N

M

F

S

W

A

F

A

Q

L

V

I

G

G

Y

M

A

V

V

F

G

G

F

A

V

L

L

G

Q

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G
|
G

R

P

L

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A

D
|
D

R

R

I

F

G
|
G

V

R

K

K

R

G

V

V

F

L

F

V

C

G

A

A

V

V

L

L

L

V

W

F

S

G

L

G

A

F

T

S

G

L

A

A

H

S

G

A

L

Y

A

A

T

T

S

N

A

V

L

D

G

Q

F

L

M

L

V

V

C

L

R
|
R

F

F

I

L

L

T

G

G

L

L

T

G

E

L

A

G

A

A

N

G

Y

M

P

P

A

N

C

A

V

T

K

T

T

L

R

S

L
x
E

W

Y

F

T

P

P

A

E

G

R

E

L

R

K

A

S

V

L

A

L

T

V

G

T

I

S

F

M

N

F

A

C

G

G

T

F

N

N

V

L

G

G

A

M

M

A

F

G

T

G

P

G

M

F

L

I

L

S

P

A

L

K

I

M

L

I

H

P

V

A

W

Y

G

G

W

W

Q
x
H

A

S

D41 (≠ N53) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
D44 (= D56) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
G85 (= G97) mutation to V: Abolishes 4-HBA transport and chemotaxis.
D89 (= D101) mutation to N: Abolishes 4-HBA transport and chemotaxis.
G92 (= G104) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
R124 (= R136) mutation to A: Abolishes 4-HBA transport.
E144 (≠ L156) mutation to A: Strong decrease in 4-HBA transport.
H183 (≠ Q195) mutation to A: Decrease in 4-HBA transport and chemotaxis.

Sites not aligning to the query:

323 D→N: Abolishes 4-HBA transport and chemotaxis.
328 H→A: Decrease in 4-HBA transport and chemotaxis.; H→R: Decrease in 4-HBA transport and loss of chemotaxis.
386 R→A: Strong decrease in 4-HBA transport.
398 R→A: Abolishes 4-HBA transport.
444 H→A: No change in 4-HBA transport and chemotaxis.

Query Sequence

>AO353_06195 FitnessBrowser__pseudo3_N2E3:AO353_06195
MIPSQSSHTESARMASGLGVATGGIGDKIRGAMAVGKTRWGMLALVFFATTLNYIDRAAL
GVMQPILAKEMSWTAMDYANINFWFQVGYAVGFVLQGRLIDRIGVKRVFFCAVLLWSLAT
GAHGLATSALGFMVCRFILGLTEAANYPACVKTTRLWFPAGERAVATGIFNAGTNVGAMF
TPMLLPLILHVWGWQAAFLCMSALGGIWLVFWGLKYFNPEDHPSVKQSELDYIQNEAEPE
QARVPFSRILRMRGTWAFALAYSMTAPVFWFYLYWLPPFLNQQYNLGINVTQMGIPLIII
YMTADFGSVGGGILSSFLIGRGVNPIKARLMSMLLFACCIIGVIMAAGSSNLWVAVFAIS
LAIGAHQAWTANIWSLVMDYTPKHMMSTVFGFGGMCAAIGGMFMTQLVGHILTITNNNYT
VLFTMIPAMYFIALIWLYFMAPRKIPNLED

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory