SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO353_07785 FitnessBrowser__pseudo3_N2E3:AO353_07785 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

B5Z7I3 Ergothioneine transport permease/ergothioneine binding protein EgtU from Helicobacter pylori (strain G27) (see paper)
27% identity, 62% coverage: 98:270/281 of query aligns to 64:236/553 of B5Z7I3

query
sites
B5Z7I3

V

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Y

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Y

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I

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L

-

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-

G

G

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G

G

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P

N

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Y

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Sites not aligning to the query:

390 Y→A: Disrupts EGT binding.; Y→F: Retains EGT binding.
454 R→A: Retains EGT binding.; R→E: Disrupts EGT binding.

Query Sequence

>AO353_07785 FitnessBrowser__pseudo3_N2E3:AO353_07785
MLIDQKIPLGQYIAAFVEWLTQHGASTFDAIATTLETMIHGVTFALTWFNPLALIGLIAL
LAHFIQRKWGLTAFVIASFLLILNLGYWQETMETLAQVLFATFVCVIIGVPLGIVAAHKP
MFYTMMRPVLDLMQTVPTFVYLIPTLTLFGLGVVPGLISTVVFAIAAPIRLTYLGIRDVP
QELMDAGKAFGCSRRQLLSRIELPHAMPSIAAGITQCIMLSLSMVVIAALVGADGLGKPV
VNALNTADIALGFEAGLAIVLLAIMLDRICKQPDAKVGGDA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory