SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO353_10800 AO353_10800 alcohol dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 12 hits to proteins with known functional sites (download)

Q47945 Alcohol dehydrogenase (quinone), cytochrome c subunit; ADH; Alcohol dehydrogenase (quinone), subunit II; Cytochrome c-553; Cytochrome c553; Ethanol:Q2 reductase; G3-ADH subunit II; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 from Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) (see paper)
54% identity, 95% coverage: 20:434/435 of query aligns to 36:449/478 of Q47945

query
sites
Q47945

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Q37 (≠ E21) modified: Pyrrolidone carboxylic acid

Sites not aligning to the query:

1:36 signal peptide

7w2jC Cryo-em structure of membrane-bound fructose dehydrogenase from gluconobacter japonicus
38% identity, 89% coverage: 28:416/435 of query aligns to 1:386/418 of 7w2jC

query
sites
7w2jC

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binding heme c: C13 (= C40), C16 (= C43), H17 (= H44), I39 (= I65), S41 (= S67), T42 (= T68), N43 (= N69), I44 (= I70), I52 (≠ L77), Y55 (= Y80), F60 (= F85), A63 (= A88), L75 (= L100), Y76 (= Y101), A78 (= A103), M79 (= M104), P80 (= P105), Y84 (= Y109), R122 (= R147), H161 (= H186), C162 (= C187), C165 (= C190), H166 (= H191), W189 (= W221), R190 (≠ I222), A191 (= A223), P192 (≠ K224), I194 (≠ L226), W205 (= W237), Y213 (≠ F245), L214 (= L246), R223 (≠ A255), P227 (≠ G259), M228 (= M260), L236 (≠ M268), V289 (≠ N322), C290 (= C323), C293 (= C326), H294 (= H327), Y305 (≠ V337), Y306 (≠ F338), P307 (= P339), L309 (= L341), N312 (= N344), T313 (≠ P345), T314 (≠ V346), T314 (≠ V346), Q317 (≠ S349), D322 (≠ S354), L323 (= L355), S326 (≠ I358), I327 (≠ V359), V331 (≠ A363), R333 (≠ L365), I340 (≠ F375), M342 (= M377), P343 (= P378), F345 (= F380)

8hddB Complex structure of catalytic, small, and a partial electron transfer subunits from burkholderia cepacia fad glucose dehydrogenase
30% identity, 23% coverage: 316:416/435 of query aligns to 23:121/121 of 8hddB

query
sites
8hddB

A

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binding protoporphyrin ix containing fe: N29 (= N322), C30 (= C323), C33 (= C326), H34 (= H327), Y45 (≠ V337), Y46 (≠ F338), P47 (= P339), T54 (≠ V346), V66 (≠ I358), I67 (≠ V359), R73 (≠ T368), I80 (≠ F375), M82 (= M377), P83 (= P378), F85 (= F380)

2zooA Crystal structure of nitrite reductase from pseudoalteromonas haloplanktis tac125
32% identity, 25% coverage: 308:416/435 of query aligns to 332:438/438 of 2zooA

query
sites
2zooA

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binding beta-D-fructofuranose: P359 (≠ T335), G360 (≠ R336), A361 (≠ V337)
binding protoporphyrin ix containing fe: N346 (= N322), C347 (= C323), C350 (= C326), H351 (= H327), F362 (= F338), P363 (= P339), P364 (≠ A340), L365 (= L341), S368 (≠ N344), Y370 (≠ V346), L371 (= L347), I382 (≠ V359), L386 (≠ A363), S387 (≠ T364), G388 (≠ L365), I390 (vs. gap), V392 (≠ T368), Y397 (≠ S373), N398 (≠ T374), G399 (≠ F375), V400 (≠ T376), M401 (= M377), M404 (≠ W382), V414 (= V392)

Sites not aligning to the query:

active site: 81, 84, 86, 121, 122, 130, 135, 227, 249, 250, 276
binding copper (ii) ion: 81, 86, 121, 122, 130, 135

6fjaA Crystal structure of t2d three-domain heme-cu nitrite reductase from ralstonia pickettii
30% identity, 26% coverage: 302:416/435 of query aligns to 339:452/455 of 6fjaA

query
sites
6fjaA

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K

A
x
I

T

K

H
x
V

T

N

A

G

P

Q

S

E

T
x
Y

F
x
D

T
x
S

M
x
V

P
x
M

A

P

F

P

A
x
M

W

T

R

Q

L

L

S

N

D

D

Q

D

E

E

V

V

A

A

D

N

V
x
I

V

L

N

T

F

Y

I

V

R

L

T

N

S

S

W

W

G

D

N

N

K

P

G

G

A

G

Q

R

I

V

K

S

P

A

A

E

D

D

V

V

A

K

D

K

V

V

R

R

binding protoporphyrin ix containing fe: T359 (≠ N322), C360 (= C323), C363 (= C326), H364 (= H327), P376 (= P339), P377 (≠ A340), L378 (= L341), S381 (≠ N344), F383 (≠ V346), L384 (= L347), I394 (= I358), V395 (= V359), L399 (≠ A363), N400 (≠ T364), G401 (≠ L365), I403 (≠ A367), V405 (≠ H369), Y410 (≠ T374), D411 (≠ F375), S412 (≠ T376), V413 (≠ M377), M414 (≠ P378), M417 (≠ A381), I428 (≠ V392)

Sites not aligning to the query:

active site: 90, 93, 95, 130, 131, 139, 144, 236, 258, 259, 285
binding copper (ii) ion: 90, 131, 139, 144

4ax3D Structure of three-domain heme-cu nitrite reductase from ralstonia pickettii at 1.6 a resolution (see paper)
30% identity, 26% coverage: 302:416/435 of query aligns to 342:455/457 of 4ax3D

query
sites
4ax3D

V

V

A

S

Q

G

A

T

L

L

W

T

K

V

G

Q

D

D

D

Q

S

V

K

Q

P

A

G

G

A

R

S

A

V

L

Y

F

I

A

D

G

N
x
T

C
|
C

A

S

A

V

C
|
C

H
|
H

R

Q

T

G

D

N

G

G

H

A

G

G

Y

L

T

P

R

G

V

V

F

F

P
|
P

A
x
P

L
|
L

A

A

G

K

N
x
S

P

D

V
x
F

L
|
L

Q

A

S

A

D

D

D

-

P

P

V

K

S

R

L

A

I

M

N

N

I
|
I

V
|
V

L

L

K

H

G

G

A
x
L

T
x
N

L
x
G

P

K

A
x
I

T

K

H
x
V

T

N

A

G

P

Q

S

E

T
x
Y

F
x
D

T
x
S

M
x
V

P
x
M

A

P

F

P

A
x
M

W

T

R

Q

L

L

S

N

D

D

Q

D

E

E

V

V

A

A

D

N

V
x
I

V

L

N

T

F

Y

I

V

R

L

T

N

S

S

W

W

G

D

N

N

K

P

G

G

A

G

Q

R

I

V

K

S

P

A

A

E

D

D

V

V

A

K

D

K

V

V

R

R

binding heme c: T362 (≠ N322), C363 (= C323), C366 (= C326), H367 (= H327), P379 (= P339), P380 (≠ A340), L381 (= L341), S384 (≠ N344), F386 (≠ V346), L387 (= L347), I397 (= I358), V398 (= V359), L402 (≠ A363), N403 (≠ T364), G404 (≠ L365), I406 (≠ A367), V408 (≠ H369), Y413 (≠ T374), D414 (≠ F375), S415 (≠ T376), V416 (≠ M377), M417 (≠ P378), M420 (≠ A381), I431 (≠ V392)

Sites not aligning to the query:

active site: 93, 96, 98, 133, 134, 142, 147, 239, 261, 262, 288
binding copper (ii) ion: 93, 98, 133, 134, 142, 147

5ocbA Crystal structure of nitric oxide bound d97n mutant of three-domain heme-cu nitrite reductase from ralstonia pickettii
30% identity, 26% coverage: 302:416/435 of query aligns to 340:453/456 of 5ocbA

query
sites
5ocbA

V

V

A

S

Q

G

A

T

L

L

W

T

K

V

G

Q

D

D

D

Q

S

V

K

Q

P

A

G

G

A

R

S

A

V

L

Y

F

I

A

D

G

N
x
T

C
|
C

A

S

A

V

C
|
C

H
|
H

R

Q

T

G

D

N

G

G

H

A

G

G

Y

L

T

P

R

G

V

V

F

F

P
|
P

A
x
P

L
|
L

A

A

G

K

N
x
S

P

D

V
x
F

L
|
L

Q

A

S

A

D

D

D

-

P

P

V

K

S

R

L

A

I

M

N

N

I
|
I

V
|
V

L

L

K

H

G

G

A
x
L

T
x
N

L
x
G

P

K

A
x
I

T

K

H
x
V

T

N

A

G

P

Q

S

E

T
x
Y

F
x
D

T
x
S

M
x
V

P
x
M

A

P

F

P

A
x
M

W

T

R

Q

L

L

S

N

D

D

Q

D

E

E

V

V

A

A

D

N

V
x
I

V

L

N

T

F

Y

I

V

R

L

T

N

S

S

W

W

G

D

N

N

K

P

G

G

A

G

Q

R

I

V

K

S

P

A

A

E

D

D

V

V

A

K

D

K

V

V

R

R

binding heme c: T360 (≠ N322), C361 (= C323), C364 (= C326), H365 (= H327), P377 (= P339), P378 (≠ A340), L379 (= L341), S382 (≠ N344), F384 (≠ V346), L385 (= L347), I395 (= I358), V396 (= V359), L400 (≠ A363), N401 (≠ T364), G402 (≠ L365), I404 (≠ A367), V406 (≠ H369), Y411 (≠ T374), D412 (≠ F375), S413 (≠ T376), V414 (≠ M377), M415 (≠ P378), M418 (≠ A381), I429 (≠ V392)

Sites not aligning to the query:

active site: 91, 94, 96, 131, 132, 140, 145, 237, 259, 260, 286
binding copper (ii) ion: 91, 96, 131, 132, 140, 145
binding nitric oxide: 94, 131

5oboA Crystal structure of nitrite bound d97n mutant of three-domain heme-cu nitrite reductase from ralstonia pickettii
30% identity, 26% coverage: 302:416/435 of query aligns to 340:453/456 of 5oboA

query
sites
5oboA

V

V

A

S

Q

G

A

T

L

L

W

T

K

V

G

Q

D

D

D

Q

S

V

K

Q

P

A

G

G

A

R

S

A

V

L

Y

F

I

A

D

G

N
x
T

C
|
C

A

S

A

V

C
|
C

H
|
H

R

Q

T

G

D

N

G

G

H

A

G

G

Y

L

T

P

R

G

V

V

F

F

P
|
P

A
x
P

L
|
L

A

A

G

K

N
x
S

P

D

V
x
F

L
|
L

Q

A

S

A

D

D

D

-

P

P

V

K

S

R

L

A

I

M

N

N

I
|
I

V
|
V

L

L

K

H

G

G

A
x
L

T
x
N

L
x
G

P

K

A
x
I

T

K

H
x
V

T

N

A

G

P

Q

S

E

T
x
Y

F
x
D

T
x
S

M
x
V

P
x
M

A

P

F

P

A
x
M

W

T

R

Q

L

L

S

N

D

D

Q

D

E

E

V

V

A

A

D

N

V

I

V

L

N

T

F

Y

I

V

R

L

T

N

S

S

W

W

G

D

N

N

K

P

G

G

A

G

Q

R

I

V

K

S

P

A

A

E

D

D

V

V

A

K

D

K

V

V

R

R

binding heme c: T360 (≠ N322), C361 (= C323), C364 (= C326), H365 (= H327), P377 (= P339), P378 (≠ A340), L379 (= L341), S382 (≠ N344), F384 (≠ V346), L385 (= L347), I395 (= I358), V396 (= V359), L400 (≠ A363), N401 (≠ T364), G402 (≠ L365), I404 (≠ A367), V406 (≠ H369), Y411 (≠ T374), D412 (≠ F375), S413 (≠ T376), V414 (≠ M377), M415 (≠ P378), M418 (≠ A381)

Sites not aligning to the query:

active site: 91, 94, 96, 131, 132, 140, 145, 237, 259, 260, 286
binding copper (ii) ion: 91, 96, 131, 132, 140, 145
binding nitrite ion: 94, 96, 131

1dt1A Thermus thermophilus cytochrome c552 synthesized by escherichia coli (see paper)
31% identity, 25% coverage: 315:423/435 of query aligns to 2:114/129 of 1dt1A

query
sites
1dt1A

G

G

A

A

S

K

V

I

Y

Y

I

A

D

-

N
x
Q

C
|
C

A

A

A

G

C
|
C

H
|
H

R

Q

T

Q

D

N

G

G

H

Q

G

G

Y

I

T

P

R

G

V

A

F
|
F

P
|
P

A

P

L
|
L

A

A

G

G

N
x
H

-

V

-

A

P

E

V
x
I

L

L

Q

A

S

K

D

E

D

G

P

G

V

R

S

E

L
x
Y

I

L

N

I

I

L

V
|
V

L
|
L

K

L

G

Y

A

G

T
x
L

L
x
Q

P
x
G

A

Q

T
x
I

H

E

T

V

A

K

P

G

S

M

T

K

F
x
Y

-
x
N

-
x
G

T
x
V

M
|
M

P

S

A

S

F
|
F

A

A

W

-

R

Q

L

L

S

K

D

D

Q

E

E

E

V

I

A

A

D

A

V

V

V

L

N

N

F

H

I
|
I

R

A

T

T

S

A

W

W

G

G

N

D

-

A

-

K

K

K

G

V

A

K

Q

G

I

F

K

K

P

P

A

F

D

T

V

A

A

E

D

E

V

V

R

K

K

K

D

L

E

R

V

A

K

K

S

K

M

L

binding heme c: Q8 (≠ N322), C9 (= C323), C12 (= C326), H13 (= H327), F24 (= F338), P25 (= P339), L27 (= L341), H30 (≠ N344), I34 (≠ V346), Y43 (≠ L355), V47 (= V359), L48 (= L360), L52 (≠ T364), Q53 (≠ L365), G54 (≠ P366), I56 (≠ T368), Y63 (≠ F375), N64 (vs. gap), G65 (vs. gap), V66 (≠ T376), M67 (= M377), F70 (= F380), I85 (= I396)

Sites not aligning to the query:

binding heme c: 123

1c52A Thermus thermophilus cytochrome-c552: a new highly thermostable cytochromE-C structure obtained by mad phasing (see paper)
31% identity, 25% coverage: 315:423/435 of query aligns to 4:116/131 of 1c52A

query
sites
1c52A

G

G

A

A

S

K

V

I

Y

Y

I

A

D

-

N

Q

C
|
C

A

A

A

G

C
|
C

H
|
H

R

Q

T

Q

D

N

G

G

H

Q

G

G

Y

I

T

P

R

G

V

A

F
|
F

P
|
P

A

P

L
|
L

A

A

G

G

N
x
H

-

V

-

A

P

E

V
x
I

L

L

Q

A

S

K

D

E

D

G

P

G

V

R

S

E

L
x
Y

I

L

N

I

I

L

V
|
V

L
|
L

K

L

G

Y

A

G

T
x
L

L
x
Q

P
x
G

A

Q

T
x
I

H

E

T

V

A

K

P

G

S

M

T

K

F
x
Y

-
x
N

-
x
G

T
x
V

M
|
M

P

S

A

S

F
|
F

A

A

W

-

R

Q

L

L

S

K

D

D

Q

E

E

E

V

I

A

A

D

A

V

V

V

L

N

N

F

H

I
|
I

R

A

T

T

S

A

W

W

G

G

N

D

-

A

-

K

K

K

G

V

A

K

Q

G

I

F

K

K

P

P

A

F

D

T

V

A

A

E

D

E

V

V

R

K

K

K

D

L

E

R

V

A

K

K

S

K

M

L

binding protoporphyrin ix containing fe: C11 (= C323), C14 (= C326), H15 (= H327), F26 (= F338), P27 (= P339), L29 (= L341), H32 (≠ N344), I36 (≠ V346), Y45 (≠ L355), V49 (= V359), L50 (= L360), L54 (≠ T364), Q55 (≠ L365), G56 (≠ P366), I58 (≠ T368), Y65 (≠ F375), N66 (vs. gap), G67 (vs. gap), V68 (≠ T376), M69 (= M377), F72 (= F380), I87 (= I396)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 125

1r0qA Characterization of the conversion of the malformed, recombinant cytochrome rc552 to a 2-formyl-4-vinyl (spirographis) heme (see paper)
31% identity, 25% coverage: 315:423/435 of query aligns to 3:115/130 of 1r0qA

query
sites
1r0qA

G

G

A

A

S

K

V

I

Y
|
Y

I

A

D

-

N

Q

C
|
C

A

A

A

G

C
|
C

H
|
H

R

Q

T

Q

D

N

G

G

H

Q

G

G

Y

I

T

P

R

G

V

A

F
|
F

P
|
P

A

P

L
|
L

A

A

G

G

N
x
H

-

V

-

A

P

E

V
x
I

L

L

Q

A

S

K

D

E

D

G

P

G

V

R

S

E

L
x
Y

I

L

N

I

I

L

V
|
V

L
|
L

K

L

G

Y

A

G

T
x
L

L
x
Q

P
x
G

A

Q

T
x
I

H

E

T

V

A

K

P

G

S

M

T

K

F
x
Y

-
x
N

-
x
G

T
x
V

M
|
M

P

S

A

S

F
|
F

A

A

W

-

R

Q

L

L

S

K

D

D

Q

E

E

E

V

I

A

A

D

A

V
|
V

V

L

N

N

F

H

I
|
I

R

A

T

T

S

A

W

W

G

G

N

D

-

A

-

K

K

K

G

V

A

K

Q

G

I

F

K

K

P

P

A

F

D

T

V

A

A

E

D

E

V

V

R

K

K

K

D

L

E

R

V

A

K

K

S

K

M

L

binding 2-formyl-protoporphryn ix: Y7 (= Y319), C10 (= C323), C13 (= C326), H14 (= H327), F25 (= F338), P26 (= P339), L28 (= L341), H31 (≠ N344), I35 (≠ V346), Y44 (≠ L355), V48 (= V359), L49 (= L360), L53 (≠ T364), Q54 (≠ L365), G55 (≠ P366), I57 (≠ T368), Y64 (≠ F375), N65 (vs. gap), G66 (vs. gap), V67 (≠ T376), M68 (= M377), F71 (= F380), V82 (= V392), I86 (= I396)

Sites not aligning to the query:

binding 2-formyl-protoporphryn ix: 124

1qyzA Characterization of the malformed, recombinant cytochrome rc552 (see paper)
31% identity, 25% coverage: 315:423/435 of query aligns to 3:115/130 of 1qyzA

query
sites
1qyzA

G

G

A

A

S

K

V

I

Y
|
Y

I

A

D

-

N

Q

C
|
C

A

A

A

G

C
|
C

H
|
H

R

Q

T

Q

D

N

G

G

H

Q

G

G

Y

I

T

P

R

G

V

A

F
|
F

P
|
P

A

P

L
|
L

A

A

G

G

N
x
H

-

V

-

A

P

E

V
x
I

L

L

Q

A

S

K

D

E

D

G

P

G

V

R

S

E

L
x
Y

I

L

N

I

I

L

V
|
V

L
|
L

K

L

G

Y

A

G

T
x
L

L
x
Q

P
x
G

A

Q

T
x
I

H

E

T

V

A

K

P

G

S

M

T

K

F
x
Y

-
x
N

-
x
G

T
x
V

M
|
M

P

S

A

S

F
|
F

A

A

W

-

R

Q

L

L

S

K

D

D

Q

E

E

E

V

I

A

A

D

A

V

V

V

L

N

N

F

H

I
|
I

R

A

T

T

S

A

W

W

G

G

N

D

-

A

-

K

K

K

G

V

A

K

Q

G

I

F

K

K

P

P

A

F

D

T

V

A

A

E

D

E

V

V

R

K

K

K

D

L

E

R

V

A

K

K

S

K

M

L

binding 2-acetyl-protoporphyrin ix: Y7 (= Y319), C10 (= C323), C13 (= C326), H14 (= H327), F25 (= F338), P26 (= P339), L28 (= L341), H31 (≠ N344), I35 (≠ V346), Y44 (≠ L355), V48 (= V359), L49 (= L360), L53 (≠ T364), Q54 (≠ L365), G55 (≠ P366), I57 (≠ T368), Y64 (≠ F375), N65 (vs. gap), G66 (vs. gap), V67 (≠ T376), M68 (= M377), F71 (= F380), I86 (= I396)

Sites not aligning to the query:

binding 2-acetyl-protoporphyrin ix: 124

Query Sequence

>AO353_10800 AO353_10800 alcohol dehydrogenase
MKNLVIATLALLGSCSISAAEDASQALIKQGEYLARAGDCVACHTAKGGKPFAGGLPMET
PIGTIYSTNITPDKTGLGDYSFDDFDKAVRHGVAKNGSTLYPAMPYPSYARVSDADMKAL
YAYFMKGVAPVTQENKGSDIPWPLSMRWPLTGWRWMFAPSVVAYQPADGKEAAISRGAYL
VEGLGHCGACHTPRALTMQEKALSANEGSAFLSGSAPLEGWIAKSLRGDHKDGLGSWSEE
QLVQFLKTGRSDRSAVFGGMSDVVEHSMQYMSDDDLTAIARYLKSLPANDPNDQPHPYDP
QVAQALWKGDDSKPGASVYIDNCAACHRTDGHGYTRVFPALAGNPVLQSDDPVSLINIVL
KGATLPATHTAPSTFTMPAFAWRLSDQEVADVVNFIRTSWGNKGAQIKPADVADVRKDEV
KSMPADKSGKLTSSN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory