SitesBLAST

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
42% identity, 91% coverage: 1:298/329 of query aligns to 5:300/353 of 1vciA

query
sites
1vciA

M

V

S

E

I

V

E

K

V

L

R

E

N

N

V

L

S

T

K

K

N

R

F
|
F

N

G

A

N

F
|
F

K

T

A

A

L

V

N

N

S

K

I

L

N

N

L

L

D

T

I

I

Q

K

S

D

G

G

E

E

L

F

V

L

A

V

L

L

G

G

P

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

T
|
T

L

T

L

L

R

R

I

M

I

I

A

A

G

G

L

L

E

E

T

E

P

P

D

T

A

E

G

G

S

R

I

I

V

Y

F

F

H

G

G

D

E

R

D

D

V

V

S

T

G

Y

H

L

D

P

V

P

R

K

D

D

R

R

N

N

V

I

G

S

F

M

V

V

F

F

Q

Q

H

H

Y

-

A

-

L

-

F

-

R

-

H

-

M

M

T

T

V

V

F

Y

D

E

N

N

V

I

A

A

F

F

G

P

L

L

R

K

M

K

K

F

P

P

K

K

N

D

Q

E

R

-

P

-

S

-

E

-

S

-

Q

-

I

I

A

D

V

K

K

R

V

V

H

R

E

W

L

A

N

E

M

L

V

L

Q

Q

L

I

D

E

W

E

L

L

S

L

D

N

R

R

Y

Y

P

P

E

A

Q

Q

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

I

V

A

A

L

V

A

A

R

R

A

A

L

I

A

V

V

V

E

E

P

P

K

D

V

V

L

L

L

M

D

D

E

E

P

P

F

L

G

S

A

N

L

L

D

D

A

A

K

K

V

L

R

R

K

V

E

A

L

M

R

R

R

A

W

E

L

I

A

K

R

K

L

L

H

Q

E

Q

D

K

I

L

N

K

L

V

T

T

S

T

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

E

T

V

M

A

G

D

D

R

R

I

I

V

A

V

V

M

M

N

N

K

R

G

G

V

Q

I

L

E

L

Q

Q

I

I

G

G

S

S

P

P

G

T

D

E

V

V

Y

Y

E

L

N

R

P

P

A

N

S

S

D

V

F

F

V

V

Y

A

H

T

F

F

L

I

G

G

-

A

-

P

D

E

S

M

N

N

R

I

L

L

H

E

L

V

G

S

-

V

D

G

D

D

N

G

H

Y

V

L

L

E

F

G

R

R

P

G

H

F

E

R

V

I

S

E

L

L

S

P

R

Q

T

M

E

D

L

L

A

L

D

K

H

D

H

Y

A

V

A

G

Q

K

-

T

-

V

V

L

R

F

D

G

I

I

R

R

P

P

-

E

-

H

-

M

-

T

-

V

L

E

G

G

A

V

T

H

T

M

R

K

I

R

T

T

L

A

K

R

V

L

E

I

G

G

Q

K

S

V

E

D

L

F

I

V

E

E

A

A

binding adenosine-5'-triphosphate: F16 (= F12), F19 (= F15), S41 (= S37), G42 (= G38), G44 (= G40), K45 (= K41), T46 (= T42), T47 (= T43)

2d62A Crystal structure of multiple sugar binding transport atp-binding protein
45% identity, 72% coverage: 3:238/329 of query aligns to 7:244/375 of 2d62A

query
sites
2d62A

I

V

E

K

V

L

R

I

N

N

V

I

S

W

K

K

N

R

F

F

N

G

A

D

F

V

K

T

A

A

L

V

N

K

S

D

I

L

N

S

L

L

D

E

I

I

Q

K

S

D

G

G

E

E

L

F

V

L

A

V

L

L

G

G

P

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

L

T

L

L

R

R

I

M

I

I

A

A

G

G

L

L

E

E

T

E

P

P

D

T

A

R

G

G

S

Q

I

I

V

Y

F

I

H

E

G

D

E

N

D

L

V

V

S

A

G

D

H

P

D

E

-

K

-

G

-

V

-

F

-

V

-

P

V

P

R

K

D

E

R

R

N

D

V

V

G

A

F

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

R

P

H

H

M

M

T

T

V

V

F

Y

D

D

N

N

V

I

A

A

F

F

G

P

L

L

R

K

M

L

K

R

P

-

K

-

N

-

Q

-

R

K

P

V

S

P

E

K

S

Q

Q

E

I

I

A

D

V

K

K

R

V

V

H

R

E

E

L

V

L

A

N

E

M

M

V

L

Q

G

L

L

D

T

W

E

L

L

S

L

D

N

R

R

Y

K

P

P

E

R

Q

E

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

I

V

A

A

L

L

A

G

R

R

A

A

L

I

A

I

V

R

E

R

P

P

K

K

V

V

L

F

L

L

L

M

D

D

E

E

P

P

F

L

G

S

A

N

L

L

D

D

A

A

K

K

V

L

R

R

K

V

E

K

L

M

R

R

R

A

W

E

L

L

A

K

R

K

L

L

H

Q

E

R

D

Q

I

L

N

G

L

V

T

T

S

T

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

E

T

V

M

A

G

D

D

R

R

I

I

V

A

V

V

M

M

N

N

K

K

G

G

V

E

I

L

E

Q

Q

Q

I

V

G

G

S

T

P

P

G

D

D

E

V

V

Y

Y

E

Y

N

K

P

P

A

V

S

N

D

T

F

F

V

V

Y

A

H

G

F

F

L

I

G

G

binding pyrophosphate 2-: G42 (= G38), C43 (= C39), G44 (= G40), K45 (= K41), T46 (= T42), T47 (= T43)

1g291 Malk (see paper)
45% identity, 71% coverage: 7:238/329 of query aligns to 8:241/372 of 1g291

query
sites
1g291

N

D

V

V

S

W

K

K

N

V

F

F

N

G

A

E

F

V

K

T

A

A

L

V

N

R

S

E

I

M

N

S

L

L

D

E

I

V

Q

K

S

D

G

G

E

E

L

F

V

M

A

I

L

L

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

L

T

L

L

R

R

I

M

I

I

A

A

G

G

L

L

E

E

T

E

P

P

D

S

A

R

G

G

S

Q

I

I

V

Y

F

I

H

G

G

D

E

K

D

L

V

V

S

A

G
x
D

H

P

D
x
E

-
x
K

-

G

-

I

-

F

-

V

-

P

V

P

R
x
K

D
|
D

R

R

N

D

V

I

G

A

F

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

R

P

H

H

M

M

T

T

V

V

F

Y

D

D

N

N

V

I

A

A

F

F

G

P

L

L

R

K

M

L

K

R

P

-

K

-

N

-

Q

-

R

K

P

V

S

P

E

R

S

Q

Q

E

I

I

A

D

V

Q

K

R

V

V

H

R

E

E

L

V

L

A

N

E

M

L

V

L

Q

G

L

L

D

T

W

E

L

L

S

L

D

N

R

R

Y

K

P

P

E

R

Q

E

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

I

V

A

A

L

L

A

G

R

R

A

A

L

I

A

V

V

R

E

K

P

P

K

Q

V

V

L

F

L

L

L

M

D

D

E

E

P

P

F

L

G

S

A

N

L

L

D

D

A

A

K

K

V

L

R

R

K

V

E

R

L

M

R

R

R

A

W

E

L

L

A

K

R

K

L

L

H

Q

E

R

D

Q

I

L

N

G

L

V

T

T

S

T

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

E

T

V

M

A

G

D

D

R

R

I

I

V

A

V

V

M

M

N

N

K

R

G

G

V

V

I

L

E

Q

Q

Q

I

V

G

G

S

S

P

P

G

D

D

E

V

V

Y

Y

E

D

N

K

P

P

A

A

S

N

D

T

F

F

V

V

Y

A

H

G

F

F

L

I

G

G

binding magnesium ion: D69 (≠ G68), E71 (≠ D70), K72 (vs. gap), K79 (≠ R72), D80 (= D73)
binding pyrophosphate 2-: S38 (= S37), G39 (= G38), C40 (= C39), G41 (= G40), K42 (= K41), T43 (= T42), T44 (= T43)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 359

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
46% identity, 72% coverage: 3:238/329 of query aligns to 4:236/393 of P9WQI3

query
sites
P9WQI3

I

I

E

V

V

L

R

D

N

H

V

V

S

N

K

K

N

S

F

Y

-

P

N

D

A

G

F

H

K

T

A

A

L

V

N

R

S

D

I

L

N

N

L

L

D

T

I

I

Q

A

S

D

G

G

E

E

L

F

V

L

A

I

L

L

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

T

L

T

L

L

R

N

I

M

I

I

A

A

G

G

L

L

E

E

T

D

P

I

D

S

A

S

G

G

S

E

I

L

V

R

F

I

H

A

G

G

E

E

D

R

V

V

S

N

G

E

H

K

D

A

V

P

R

K

D

D

R

R

N

D

V

I

G

A

F

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

R

P

H

H

M

M

T

T

V

V

F

R

D

Q

N

N

V

I

A

A

F

F

G

P

L

L

R

T

M

L

K

A

P

-

K

-

N

-

Q

-

R

K

P

M

S

R

E

K

S

A

Q

D

I

I

A

A

V

Q

K

K

V

V

H

S

E

E

L

T

L

A

N

K

M

I

V

L

Q

D

L

L

D

T

W

N

L

L

S

L

D

D

R

R

Y

K

P

P

E

S

Q

Q

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

I

V

A

A

L

M

A

G

R

R

A

A

L

I

A

V

V

R

E

H

P

P

K

K

V

A

L

F

L

L

L

M

D

D

E

E

P

P

F

L

G

S

A

N

L

L

D

D

A

A

K

K

V

L

R

R

K

V

E

Q

L

M

R

R

R

G

W

E

L

I

A

A

R

Q

L

L

H

Q

E

R

D

R

I

L

N

G

L

T

T

T

S

T

V

V

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

T

E

E

A

A

M

M

E

T

V

L

A

G

D

D

R

R

I

V

V

V

M

M

N

Y

K

G

G

G

V

I

I

A

E

Q

Q

Q

I

I

G

G

S

T

P

P

G

E

D

E

V

L

Y

Y

E

E

N

R

P

P

A

A

S

N

D

L

F

F

V

V

Y

A

H

G

F

F

L

I

G

G

H193 (= H195) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
44% identity, 72% coverage: 2:239/329 of query aligns to 2:235/374 of 2awnB

query
sites
2awnB

S

S

I

V

E

Q

V

L

R

Q

N

N

V

V

S

T

K

K

N

A

F
x
W

N

G

A

E

F

V

K

V

A
x
V

L

S

N

K

S

D

I

I

N

N

L

L

D

D

I

I

Q

H

S

E

G

G

E

E

L

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

I

M

I

I

A

A

G

G

L

L

E

E

T

T

P

I

D

T

A

S

G

G

S

D

I

L

V

F

F

I

H

G

G

E

E

K

D

R

V

M

S

N

G

D

H

T

D

P

V

P

R

A

D

E

R

R

N

G

V

V

G

G

F

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

R

P

H

H

M

L

T

S

V

V

F

A

D

E

N

N

V

M

A

S

F

F

G

G

L

L

R

K

M

L

K

A

P

G

K

A

N

K

Q

K

R

-

P

-

S

-

E

-

S

E

Q

V

I

I

A

N

V

Q

K

R

V

V

H

N

E

Q

L

V

L

A

N

E

M

V

V

L

Q

Q

L

L

D

A

W

H

L

L

S

L

D

D

R

R

Y

K

P

P

E

K

Q

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

I

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

V

A

E

E

P

P

K

S

V

V

L

F

L

L

D

D

E

E

P

P

F

L

G

S

A

N

L

L

D

D

A

A

K

A

V

L

R

R

K

V

E

Q

L

M

R

R

R

I

W

E

L

I

A

S

R

R

L

L

H

H

E

K

D

R

I

L

N

G

L

R

T

T

S

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

E

T

V

L

A

A

D

D

R

K

I

I

V

V

M

L

N

D

K

A

G

G

V

R

I

V

E

A

Q

Q

I

V

G

G

S

K

P

P

G

L

D

E

V

L

Y

Y

E

H

N

Y

P

P

A

A

S

D

D

R

F

F

V

V

Y

A

H

G

F

F

L

I

G

G

D

S

binding adenosine-5'-diphosphate: W12 (≠ F12), V17 (≠ A17), S37 (= S37), G38 (= G38), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
44% identity, 72% coverage: 2:239/329 of query aligns to 3:236/371 of P68187

query
sites
P68187

S

S

I

V

E

Q

V

L

R

Q

N

N

V

V

S

T

K

K

N

A

F

W

N

G

A

E

F

V

K

V

A

V

L

S

N

K

S

D

I

I

N

N

L

L

D

D

I

I

Q

H

S

E

G

G

E

E

L

F

V

V

A

V

L

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

L

L

R

R

I

M

I

I

A

A

G

G

L

L

E

E

T

T

P

I

D

T

A

S

G

G

S

D

I

L

V

F

F

I

H

G

G

E

E

K

D

R

V

M

S

N

G

D

H

T

D

P

V

P

R

A

D

E

R

R

N

G

V

V

G

G

F

M

V

V

F

F

Q

Q

H

S

Y

Y

A
|
A

L

L

F

Y

R

P

H

H

M

L

T

S

V

V

F

A

D

E

N

N

V

M

A

S

F

F

G

G

L

L

R

K

M

L

K

A

P

G

K

A

N
x
K

Q

K

R

-

P

-

S

-

E

-

S

E

Q

V

I

I

A

N

V

Q

K

R

V
|
V

H

N

E

Q

L
x
V

L

A

N
x
E

M

V

V

L

Q

Q

L

L

D
x
A

W

H

L

L

S

L

D

D

R

R

Y

K

P

P

E

K

Q

A

L

L

S

S

G

G

G
|
G

Q

Q

R

R

Q

Q

R

R

I

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

V

A

E

E

P

P

K

S

V

V

L

F

L

L

D
|
D

E

E

P

P

F

L

G

S

A

N

L

L

D

D

A

A

K

A

V

L

R

R

K

V

E

Q

L

M

R

R

R

I

W

E

L

I

A

S

R

R

L

L

H

H

E

K

D

R

I

L

N

G

L

R

T

T

S

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

E

T

V

L

A

A

D

D

R

K

I

I

V

V

M

L

N

D

K

A

G

G

V

R

I

V

E

A

Q

Q

I

V

G

G

S

K

P

P

G

L

D

E

V

L

Y

Y

E

H

N

Y

P

P

A

A

S

D

D
x
R

F

F

V

V

Y

A

H

G

F

F

L

I

G

G

D

S

A85 (= A84) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ N105) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V117) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ L120) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ N122) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ D127) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G140) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D161) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ D231) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
44% identity, 72% coverage: 2:239/329 of query aligns to 2:235/371 of 3puyA

query
sites
3puyA

S

S

I

V

E

Q

V

L

R

Q

N

N

V

V

S

T

K

K

N

A

F
x
W

N

G

A

E

F

V

K

V

A

V

L

S

N

K

S

D

I

I

N

N

L

L

D

D

I

I

Q

H

S

E

G

G

E

E

L

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

I

M

I

I

A

A

G

G

L

L

E

E

T

T

P

I

D

T

A

S

G

G

S

D

I

L

V

F

F

I

H

G

G

E

E

K

D

R

V

M

S

N

G

D

H

T

D

P

V

P

R

A

D

E

R

R

N

G

V

V

G

G

F

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

R

P

H

H

M

L

T

S

V

V

F

A

D

E

N

N

V

M

A

S

F

F

G

G

L

L

R

K

M

L

K

A

P

G

K

A

N

K

Q

K

R

-

P

-

S

-

E

-

S

E

Q

V

I

I

A

N

V

Q

K

R

V

V

H

N

E

Q

L

V

L

A

N

E

M

V

V

L

Q

Q

L

L

D

A

W

H

L

L

S

L

D

D

R
|
R

Y

K

P

P

E

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

I

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

V

A

E

E

P

P

K

S

V

V

L

F

L

L

D

D

E
|
E

P

P

F

L

G

S

A

N

L

L

D

D

A

A

K

A

V

L

R

R

K

V

E

Q

L

M

R

R

R

I

W

E

L

I

A

S

R

R

L

L

H

H

E

K

D

R

I

L

N

G

L

R

T

T

S

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

M

M

E

T

V

L

A

A

D

D

R

K

I

I

V

V

M

L

N

D

K

A

G

G

V

R

I

V

E

A

Q

Q

I

V

G

G

S

K

P

P

G

L

D

E

V

L

Y

Y

E

H

N

Y

P

P

A

A

S

D

D

R

F

F

V

V

Y

A

H

G

F

F

L

I

G

G

D

S

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F12), S37 (= S37), G38 (= G38), C39 (= C39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), Q81 (= Q81), R128 (= R132), A132 (≠ Q136), S134 (= S138), G136 (= G140), Q137 (= Q141), E158 (= E162), H191 (= H195)
binding magnesium ion: S42 (≠ T42), Q81 (= Q81)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
44% identity, 72% coverage: 2:239/329 of query aligns to 2:235/371 of 3puxA

query
sites
3puxA

S

S

I

V

E

Q

V

L

R

Q

N

N

V

V

S

T

K

K

N

A

F
x
W

N

G

A

E

F

V

K

V

A

V

L

S

N

K

S

D

I

I

N

N

L

L

D

D

I

I

Q

H

S

E

G

G

E

E

L

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

I

M

I

I

A

A

G

G

L

L

E

E

T

T

P

I

D

T

A

S

G

G

S

D

I

L

V

F

F

I

H

G

G

E

E

K

D

R

V

M

S

N

G

D

H

T

D

P

V

P

R

A

D

E

R

R

N

G

V

V

G

G

F

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

R

P

H

H

M

L

T

S

V

V

F

A

D

E

N

N

V

M

A

S

F

F

G

G

L

L

R

K

M

L

K

A

P

G

K

A

N

K

Q

K

R

-

P

-

S

-

E

-

S

E

Q

V

I

I

A

N

V

Q

K

R

V

V

H

N

E

Q

L

V

L

A

N

E

M

V

V

L

Q

Q

L

L

D

A

W

H

L

L

S

L

D

D

R
|
R

Y

K

P

P

E

K

Q

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

I

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

V

A

E

E

P

P

K

S

V

V

L

F

L

L

D

D

E

E

P

P

F

L

G

S

A

N

L

L

D

D

A

A

K

A

V

L

R

R

K

V

E

Q

L

M

R

R

R

I

W

E

L

I

A

S

R

R

L

L

H

H

E

K

D

R

I

L

N

G

L

R

T

T

S

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

M

M

E

T

V

L

A

A

D

D

R

K

I

I

V

V

M

L

N

D

K

A

G

G

V

R

I

V

E

A

Q

Q

I

V

G

G

S

K

P

P

G

L

D

E

V

L

Y

Y

E

H

N

Y

P

P

A

A

S

D

D

R

F

F

V

V

Y

A

H

G

F

F

L

I

G

G

D

S

binding adenosine-5'-diphosphate: W12 (≠ F12), G38 (= G38), C39 (= C39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), R128 (= R132), S134 (= S138), Q137 (= Q141)
binding beryllium trifluoride ion: S37 (= S37), G38 (= G38), K41 (= K41), Q81 (= Q81), S134 (= S138), G136 (= G140), H191 (= H195)
binding magnesium ion: S42 (≠ T42), Q81 (= Q81)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
44% identity, 72% coverage: 2:239/329 of query aligns to 2:235/371 of 3puwA

query
sites
3puwA

S

S

I

V

E

Q

V

L

R

Q

N

N

V

V

S

T

K

K

N

A

F
x
W

N

G

A

E

F

V

K

V

A
x
V

L

S

N

K

S

D

I

I

N

N

L

L

D

D

I

I

Q

H

S

E

G

G

E

E

L

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

I

M

I

I

A

A

G

G

L

L

E

E

T

T

P

I

D

T

A

S

G

G

S

D

I

L

V

F

F

I

H

G

G

E

E

K

D

R

V

M

S

N

G

D

H

T

D

P

V

P

R

A

D

E

R

R

N

G

V

V

G

G

F

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

R

P

H

H

M

L

T

S

V

V

F

A

D

E

N

N

V

M

A

S

F

F

G

G

L

L

R

K

M

L

K

A

P

G

K

A

N

K

Q

K

R

-

P

-

S

-

E

-

S

E

Q

V

I

I

A

N

V

Q

K

R

V

V

H

N

E

Q

L

V

L

A

N

E

M

V

V

L

Q

Q

L

L

D

A

W

H

L

L

S

L

D

D

R
|
R

Y

K

P

P

E

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

I

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

V

A

E

E

P

P

K

S

V

V

L

F

L

L

D

D

E
|
E

P

P

F

L

G

S

A

N

L

L

D

D

A

A

K

A

V

L

R

R

K

V

E

Q

L

M

R

R

R

I

W

E

L

I

A

S

R

R

L

L

H

H

E

K

D

R

I

L

N

G

L

R

T

T

S

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

M

M

E

T

V

L

A

A

D

D

R

K

I

I

V

V

M

L

N

D

K

A

G

G

V

R

I

V

E

A

Q

Q

I

V

G

G

S

K

P

P

G

L

D

E

V

L

Y

Y

E

H

N

Y

P

P

A

A

S

D

D

R

F

F

V

V

Y

A

H

G

F

F

L

I

G

G

D

S

binding adenosine-5'-diphosphate: W12 (≠ F12), V17 (≠ A17), G38 (= G38), C39 (= C39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), R128 (= R132), A132 (≠ Q136), S134 (= S138), Q137 (= Q141)
binding tetrafluoroaluminate ion: S37 (= S37), G38 (= G38), K41 (= K41), Q81 (= Q81), S134 (= S138), G135 (= G139), G136 (= G140), E158 (= E162), H191 (= H195)
binding magnesium ion: S42 (≠ T42), Q81 (= Q81)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
44% identity, 72% coverage: 2:239/329 of query aligns to 2:235/371 of 3puvA

query
sites
3puvA

S

S

I

V

E

Q

V

L

R

Q

N

N

V

V

S

T

K

K

N

A

F
x
W

N

G

A

E

F

V

K

V

A
x
V

L

S

N

K

S

D

I

I

N

N

L

L

D

D

I

I

Q

H

S

E

G

G

E

E

L

F

V

V

A

V

L

F

L

V

G

G

P

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

I

M

I

I

A

A

G

G

L

L

E

E

T

T

P

I

D

T

A

S

G

G

S

D

I

L

V

F

F

I

H

G

G

E

E

K

D

R

V

M

S

N

G

D

H

T

D

P

V

P

R

A

D

E

R

R

N

G

V

V

G

G

F

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

R

P

H

H

M

L

T

S

V

V

F

A

D

E

N

N

V

M

A

S

F

F

G

G

L

L

R

K

M

L

K

A

P

G

K

A

N

K

Q

K

R

-

P

-

S

-

E

-

S

E

Q

V

I

I

A

N

V

Q

K

R

V

V

H

N

E

Q

L

V

L

A

N

E

M

V

V

L

Q

Q

L

L

D

A

W

H

L

L

S

L

D

D

R
|
R

Y

K

P

P

E

K

Q
x
A

L

L

S
|
S

G

G

Q
|
Q

R

R

Q

Q

R

R

I

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

V

A

E

E

P

P

K

S

V

V

L

F

L

L

D

D

E

E

P

P

F

L

G

S

A

N

L

L

D

D

A

A

K

A

V

L

R

R

K

V

E

Q

L

M

R

R

R

I

W

E

L

I

A

S

R

R

L

L

H

H

E

K

D

R

I

L

N

G

L

R

T

T

S

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

E

T

V

L

A

A

D

D

R

K

I

I

V

V

M

L

N

D

K

A

G

G

V

R

I

V

E

A

Q

Q

I

V

G

G

S

K

P

P

G

L

D

E

V

L

Y

Y

E

H

N

Y

P

P

A

A

S

D

D

R

F

F

V

V

Y

A

H

G

F

F

L

I

G

G

D

S

binding adenosine-5'-diphosphate: W12 (≠ F12), V17 (≠ A17), G38 (= G38), C39 (= C39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), R128 (= R132), A132 (≠ Q136), S134 (= S138), Q137 (= Q141)
binding magnesium ion: S42 (≠ T42), Q81 (= Q81)

8hprC Lpqy-sugabc in state 4 (see paper)
44% identity, 70% coverage: 8:238/329 of query aligns to 8:235/363 of 8hprC

query
sites
8hprC

V

V

S

T

K

K

N

S

F
x
Y

N

P

A

D

F

V

K

R

A

A

-

A

L

V

N

K

S

E

I

F

N

S

L

M

D

T

I

I

Q

A

S

D

G

G

E

E

L

F

V

I

A

I

L

L

L

V

G

G

P

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

T
x
S

T

T

L

T

L

L

R

N

I

M

I

I

A

A

G

G

L

L

E

E

T

E

P

I

D

T

A

S

G

G

S

E

I

L

V

R

F

I

H

G

G

G

E

E

D

R

V

V

S

N

G

E

H

K

D

A

V

P

R

K

D

D

R

R

N

D

V

I

G

A

F

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

R

P

H

H

M

M

T

T

V

V

F

R

D

Q

N

N

V

I

A

A

F

F

G

P

L

L

R

T

M

L

K

A

P

-

K

-

N

-

Q

-

R

K

P

V

S

P

E

K

S

A

Q

E

I

I

A

A

V

A

K

K

V

V

H

E

E

E

L

T

L

A

N

K

M

I

V

L

Q

D

L

L

D

S

W

E

L

L

S

L

D

D

R

R

Y

K

P

P

E

G

Q
|
Q

L

L

S

S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

I

V

A

A

L

M

A

G

R

R

A

A

L

I

A

V

V

R

E

S

P

P

K

K

V

A

L

F

L

L

L

M

D

D

E

Q

P

P

F

L

G

S

A

N

L

L

D

D

A

A

K

K

V

L

R

R

K

V

E

Q

L

M

R

R

R

A

W

E

L

I

A

S

R

R

L

L

H

Q

E

D

D

R

I

L

N

G

L

T

T

T

S

T

V

V

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

T

E

E

A

A

M

M

E

T

V

L

A

G

D

D

R

R

I

V

V

V

M

M

N

L

K

A

G

G

V

E

I

V

E

Q

Q

Q

I

I

G

G

S

T

P

P

G

D

D

E

V

L

Y

Y

E

S

N

S

P

P

A

A

S

N

D

L

F

F

V

V

Y

A

H

G

F

F

L

I

G

G

binding adenosine-5'-triphosphate: Y12 (≠ F12), S38 (= S37), G39 (= G38), G41 (= G40), K42 (= K41), S43 (≠ T42), Q82 (= Q81), Q133 (= Q136), G136 (= G139), G137 (= G140), Q138 (= Q141), H192 (= H195)
binding magnesium ion: S43 (≠ T42), Q82 (= Q81)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
44% identity, 72% coverage: 2:239/329 of query aligns to 3:236/369 of P19566

query
sites
P19566

S

S

I

V

E

Q

V

L

R

R

N

N

V

V

S

T

K

K

N

A

F

W

N

G

A

D

F

V

K

V

A

V

L

S

N

K

S

D

I

I

N

N

L

L

D

D

I

I

Q

H

S

D

G

G

E

E

L

F

V

V

A

V

L

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

L

L

R

R

I

M

I

I

A

A

G

G

L

L

E

E

T

T

P

I

D

T

A

S

G

G

S

D

I

L

V

F

F

I

H

G

G

E

E

T

D

R

V

M

S

N

G

D

H

I

D

P

V

P

R

A

D

E

R

R

N

G

V

V

G

G

F

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L
|
L

F

Y

R

P

H

H

M

L

T

S

V

V

F

A

D

E

N

N

V

M

A

S

F

F

G

G

L

L

R

K

M

L

K

A

P

G

K

A

N

K

Q

K

R

E

P

V

S

M

E

N

S

Q

Q

-

I

-

A

-

V

-

K

R

V

V

H

N

E

Q

L

V

L

A

N

E

M

V

V

L

Q

Q

L

L

D

A

W

H

L

L

S

L

D

E

R

R

Y

K

P

P

E

K

Q

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

I

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

V

A

E

E

P

P

K

R

V

V

L

F

L

L

D

D

E

E

P
|
P

F

L

G

S

A

N

L

L

D
|
D

A

A

K

A

V

L

R

R

K

V

E

Q

L

M

R

R

R

I

W

E

L

I

A

S

R

R

L

L

H

H

E

K

D

R

I

L

N

G

L

R

T

T

S

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

E

T

V

L

A

A

D

D

R

K

I

I

V

V

M

L

N

D

K

A

G

G

V

R

I

V

E

A

Q

Q

I

V

G

G

S

K

P

P

G

L

D

E

V

L

Y

Y

E

H

N

Y

P

P

A

A

S

D

D

R

F

F

V

V

Y

A

H

G

F

F

L

I

G

G

D

S

L86 (= L85) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P163) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D168) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

8hplC Lpqy-sugabc in state 1 (see paper)
45% identity, 67% coverage: 17:238/329 of query aligns to 16:233/384 of 8hplC

query
sites
8hplC

A

A

L

V

N

K

S

E

I

F

N

S

L

M

D

T

I

I

Q

A

S

D

G

G

E

E

L

F

V

I

A

I

L

L

L

V

G

G

P

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

T

L

L

R

N

I

M

I

I

A

A

G

G

L

L

E

E

T

E

P

I

D

T

A

S

G

G

S

E

I

L

V

R

F

I

H

G

G

G

E

E

D

R

V

V

S

N

G

E

H

K

D

A

V

P

R

K

D

D

R

R

N

D

V

I

G

A

F

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

R

P

H

H

M

M

T

T

V

V

F

R

D

Q

N

N

V

I

A

A

F

F

G

P

L

L

R

T

M

L

K

A

P

-

K

-

N

-

Q

-

R

K

P

V

S

P

E

K

S

A

Q

E

I

I

A

A

V

A

K

K

V

V

H

E

E

E

L

T

L

A

N

K

M

I

V

L

Q

D

L

L

D

S

W

E

L

L

S

L

D

D

R

R

Y

K

P

P

E

G

Q

Q

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

I

V

A

A

L

M

A

G

R

R

A

A

L

I

A

V

V

R

E

S

P

P

K

K

V

A

L

F

L

L

L

M

D

D

E

Q

P

P

F

L

G

S

A

N

L

L

D

D

A

A

K

K

V

L

R

R

K

V

E

Q

L

M

R

R

R

A

W

E

L

I

A

S

R

R

L

L

H

Q

E

D

D

R

I

L

N

G

L

T

T

T

S

T

V

V

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

T

E

E

A

A

M

M

E

T

V

L

A

G

D

D

R

R

I

V

V

V

M

M

N

L

K

A

G

G

V

E

I

V

E

Q

Q

Q

I

I

G

G

S

T

P

P

G

D

D

E

V

L

Y

Y

E

S

N

S

P

P

A

A

S

N

D

L

F

F

V

V

Y

A

H

G

F

F

L

I

G

G

binding adenosine-5'-triphosphate: G37 (= G38), C38 (= C39), G39 (= G40), K40 (= K41), S41 (≠ T42), T42 (= T43)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

8hprD Lpqy-sugabc in state 4 (see paper)
44% identity, 70% coverage: 8:238/329 of query aligns to 8:235/362 of 8hprD

query
sites
8hprD

V

V

S

T

K

K

N

S

F
x
Y

N

P

A

D

F

V

K

R

A

A

-

A

L

V

N

K

S

E

I

F

N

S

L

M

D

T

I

I

Q

A

S

D

G

G

E

E

L

F

V

I

A

I

L

L

L

V

G

G

P

P

S
|
S

G

G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

T

L

L

R

N

I

M

I

I

A

A

G

G

L

L

E

E

T

E

P

I

D

T

A

S

G

G

S

E

I

L

V

R

F

I

H

G

G

G

E

E

D

R

V

V

S

N

G

E

H

K

D

A

V

P

R

K

D

D

R

R

N

D

V

I

G

A

F

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

R

P

H

H

M

M

T

T

V

V

F

R

D

Q

N

N

V

I

A

A

F

F

G

P

L

L

R

T

M

L

K

A

P

-

K

-

N

-

Q

-

R

K

P

V

S

P

E

K

S

A

Q

E

I

I

A

A

V

A

K

K

V

V

H

E

E

E

L

T

L

A

N

K

M

I

V

L

Q

D

L

L

D

S

W

E

L

L

S

L

D

D

R
|
R

Y

K

P

P

E

G

Q
|
Q

L

L

S
|
S

G
|
G

Q

Q

R

R

Q

Q

R

R

I

V

A

A

L

M

A

G

R

R

A

A

L

I

A

V

V

R

E

S

P

P

K

K

V

A

L

F

L

L

L

M

D

D

E
x
Q

P

P

F

L

G

S

A

N

L

L

D

D

A

A

K

K

V

L

R

R

K

V

E

Q

L

M

R

R

R

A

W

E

L

I

A

S

R

R

L

L

H

Q

E

D

D

R

I

L

N

G

L

T

T

T

S

T

V

V

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

T

E

E

A

A

M

M

E

T

V

L

A

G

D

D

R

R

I

V

V

V

M

M

N

L

K

A

G

G

V

E

I

V

E

Q

Q

Q

I

I

G

G

S

T

P

P

G

D

D

E

V

L

Y

Y

E

S

N

S

P

P

A

A

S

N

D

L

F

F

V

V

Y

A

H

G

F

F

L

I

G

G

binding adenosine-5'-triphosphate: Y12 (≠ F12), S38 (= S37), C40 (= C39), G41 (= G40), K42 (= K41), S43 (≠ T42), T44 (= T43), Q82 (= Q81), R129 (= R132), Q133 (= Q136), S135 (= S138), G136 (= G139), G137 (= G140), Q159 (≠ E162), H192 (= H195)
binding magnesium ion: S43 (≠ T42), Q82 (= Q81)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
44% identity, 72% coverage: 3:238/329 of query aligns to 1:232/367 of 1q12A

query
sites
1q12A

I

V

E

Q

V

L

R

Q

N

N

V

V

S

T

K

K

N

A

F
x
W

N

G

A

E

F

V

K

V

A

V

L

S

N

K

S

D

I

I

N

N

L

L

D

D

I

I

Q

H

S

E

G

G

E

E

L

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

I

M

I

I

A

A

G

G

L

L

E

E

T

T

P

I

D

T

A

S

G

G

S

D

I

L

V

F

F

I

H

G

G

E

E

K

D

R

V

M

S

N

G

D

H

T

D

P

V

P

R

A

D

E

R

R

N

G

V

V

G

G

F

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

R

P

H

H

M

L

T

S

V

V

F

A

D

E

N

N

V

M

A

S

F

F

G

G

L

L

R

K

M

L

K

A

P

G

K

A

N

K

Q

K

R

-

P

-

S

-

E

-

S

E

Q

V

I

I

A

N

V

Q

K

R

V

V

H

N

E

Q

L

V

L

A

N

E

M

V

V

L

Q

Q

L

L

D

A

W

H

L

L

S

L

D

D

R
|
R

Y

K

P

P

E

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

I

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

V

A

E

E

P

P

K

S

V

V

L

F

L

L

D

D

E

E

P

P

F

L

G

S

A

N

L

L

D

D

A

A

K

A

V

L

R

R

K

V

E

Q

L

M

R

R

R

I

W

E

L

I

A

S

R

R

L

L

H

H

E

K

D

R

I

L

N

G

L

R

T

T

S

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

E

T

V

L

A

A

D

D

R

K

I

I

V

V

M

L

N

D

K

A

G

G

V

R

I

V

E

A

Q

Q

I

V

G

G

S

K

P

P

G

L

D

E

V

L

Y

Y

E

H

N

Y

P

P

A

A

S

D

D

R

F

F

V

V

Y

A

H

G

F

F

L

I

G

G

binding adenosine-5'-triphosphate: W10 (≠ F12), S35 (= S37), G36 (= G38), C37 (= C39), G38 (= G40), K39 (= K41), S40 (≠ T42), T41 (= T43), R126 (= R132), A130 (≠ Q136), S132 (= S138), G134 (= G140), Q135 (= Q141)

3d31A Modbc from methanosarcina acetivorans (see paper)
35% identity, 87% coverage: 3:287/329 of query aligns to 2:309/348 of 3d31A

query
sites
3d31A

I

I

E

E

V

I

R

E

N

S

V

L

S

S

K

R

N

K

F

W

N

K

A

N

F

F

K

-

A

S

L

L

N

D

S

N

I

L

N

S

L

L

D

K

I

V

Q

E

S

S

G

G

E

E

L

Y

V

F

A

V

L

I

L

L

G

G

P

P

S

T

G

G

C

A

G

G

K

K

T

T

T

L

L

F

L

L

R

E

I

L

I

I

A

A

G

G

L

F

E

H

T

V

P

P

D

D

A

S

G

G

S

R

I

I

V

L

F

L

H

D

G

G

E

K

D

D

V

V

S

T

G

D

H

L

D

S

V

P

R

E

D

K

R

H

N

D

V

I

G

A

F

F

V

V

F

Y

Q

Q

H

N

Y

Y

A

S

L

L

F

F

R

P

H

H

M

M

T

N

V

V

F

K

D

K

N

N

V

L

A

E

F

F

G

G

L

M

R

R

M

M

K

K

P

-

K

K

N

I

Q

K

R

D

P

P

S

K

E

-

S

-

Q

-

I

-

A

-

V

-

K

R

V

V

H

L

E

D

L

T

L

A

N

R

M

D

V

L

Q

K

L

I

D

E

W

H

L

L

S

L

D

D

R

R

Y

N

P

P

E

L

Q

T

L

L

S

S

G

G

Q

E

R

Q

Q

Q

R

R

I

V

A

A

L

L

A

A

R

R

A

A

L

L

A

V

V

T

E

N

P

P

K

K

V

I

L

L

D

D

E

E

P

P

F

L

G

S

A

A

L

L

D

D

A

P

K

R

V

T

R

Q

K

E

E

N

L

A

R

R

R

E

W

M

L

L

A

S

R

V

L

L

H

H

E

K

D

K

I

N

N

K

L

L

T

T

S

V

V

L

F

H

V

I

T

T

H

H

D

D

Q

Q

E

T

E

E

A

A

M

R

E

I

V

M

A

A

D

D

R

R

I

I

V

A

V

V

M

V

N

M

K

D

G

G

V

K

I

L

E

I

Q

Q

I

V

G

G

S

K

P

P

G

E

D

E

V

I

Y

F

E

E

N

K

P

P

A

V

S

E

D

G

F

R

V

V

Y

A

H

S

F

F

L

V

G

G

D

F

S

E

N

N

R

V

L

L

-

K

-

G

-

R

-

V

-

I

-

S

-

A

-

E

-

Q

-

G

-

L

-

R

-

I

-

R

-

V

-

G

-

E

-

V

-

I

-

D

-

A

-

G

-

D

-

M

H

E

L

V

G

G

D

D

D

Q

N

V

H

Y

V

A

L

F

F

L

R

R

P

P

H

E

E

N

V

I

S

A

L

L

S

S

R

K

-

S

-

T

-

Q

T

S

E
x
S

L

I

A

R

D

N

H

S

H

L

A

Q

A

G

Q

R

V

V

R

T

D

E

I

A

R

W

P

V

L

L

G

G

A

A

T

L

T

V

R

R

I

V

T

K

L

V

binding tungstate(vi)ion: S286 (≠ E264)

Sites not aligning to the query:

binding tungstate(vi)ion: 320, 323, 340, 341, 342

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
36% identity, 87% coverage: 3:287/329 of query aligns to 4:315/353 of 1oxvD

query
sites
1oxvD

I

I

E

I

V

V

R

K

N

N

V

V

S

S

K

K

N

V

F
|
F

N

K

A

K

F

G

K

K

-

V

A

A

L

L

N

D

S

N

I

V

N

N

L

I

D

N

I

I

Q

E

S

N

G

G

E

E

L

R

V

F

A

G

L

I

L

L

G

G

P

P

S
|
S

G
|
G

C

A

G
|
G

K
|
K

T
|
T

L

F

L

M

R

R

I

I

A

A

G

G

L

L

E

D

T

V

P

P

D

S

A

T

G

G

S

E

I

L

V

Y

F

F

H

D

G

D

E

R

D

L

V

V

S

A

G

S

H

N

D

G

-

K

-

L

-

I

-

V

-

P

V

P

R

E

D

D

R

R

N

K

V

I

G

G

F

M

V

V

F

F

Q
|
Q

H

T

Y

W

A

A

L

L

F

Y

R

P

H

N

M

L

T

T

V

A

F

F

D

E

N

N

V

I

A

A

F

F

G

P

L

L

R

-

M

-

K

-

P

-

K

T

N

N

Q

M

R

K

P

M

S

S

E

K

S

E

Q

E

I

I

A

R

V

K

K

R

V

V

H

E

E

E

L

V

L

A

N

K

M

I

V

L

Q

D

L

I

D

H

W

H

L

V

S

L

D

N

R

H

Y

F

P

P

E

R

Q

E

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

I

V

A

A

L

L

A

A

R

R

A

A

L

L

A

V

V

K

E

D

P

P

K

S

V

L

L

L

D

D

E
|
E

P

P

F

F

G

S

A

N

L

L

D

D

A

A

K

R

V

M

R

R

K

D

E

S

L

A

R

R

R

A

W

L

L

V

A

K

R

E

L

V

H

Q

E

S

D

R

I

L

N

G

L

V

T

T

S

L

V

L

F

V

V

V

T

S

H

H

D

D

Q

P

E

A

E

D

A

I

M

F

E

A

V

I

A

A

D

D

R

R

I

V

V

G

V

V

M

L

N

V

K

K

G

G

V

K

I

L

E

V

Q

Q

I

V