SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO353_13070 FitnessBrowser__pseudo3_N2E3:AO353_13070 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O53289 Phosphoserine phosphatase SerB2; PSP; PSPase; O-phosphoserine phosphohydrolase; Protein-serine/threonine phosphatase; EC 3.1.3.3; EC 3.1.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
41% identity, 97% coverage: 5:396/404 of query aligns to 7:385/409 of O53289

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O53289

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G18 (= G16) mutation to A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
G108 (≠ E110) mutation to A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
D185 (= D196) mutation to G: Completely abolishes enzymatic activity.; mutation to N: Completely abolishes enzymatic activity.
V186 (≠ M197) mutation to Q: Decreases enzymatic activity by 50%.
D187 (= D198) mutation to N: Decreases enzymatic activity by 15%.
S188 (= S199) mutation to A: No effect on enzymatic activity.
S273 (= S284) mutation to A: Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).
K318 (= K329) mutation to A: Decreases enzymatic activity by 50%.; mutation to E: Completely abolishes enzymatic activity.
D341 (= D352) mutation to G: Decreases enzymatic activity by 80%.; mutation to N: Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.
D345 (= D356) mutation to N: Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.

8a21A Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with phenylimidazole (see paper)
41% identity, 97% coverage: 5:396/404 of query aligns to 5:383/396 of 8a21A

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8a21A

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binding magnesium ion: D183 (= D196), D185 (= D198), D339 (= D352)
binding 4-phenyl-1h-imidazole: D13 (= D13), T18 (= T18), Q37 (= Q37), D185 (= D198), E192 (= E205), V193 (= V206), I194 (= I207), T211 (= T224), M215 (= M228), F221 (= F234), R228 (= R241), G273 (= G286)

8a1zA Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with 1-(2,4-dichlorophenyl)-3-hydroxyurea (see paper)
41% identity, 97% coverage: 5:396/404 of query aligns to 5:383/396 of 8a1zA

query
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8a1zA

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binding 1-(2,4-dichlorophenyl)-3-oxidanyl-urea: D185 (= D198), E192 (= E205), M215 (= M228), F221 (= F234), L225 (≠ F238), R228 (= R241), G272 (= G285), F274 (= F287), D339 (= D352)
binding magnesium ion: D183 (= D196), D185 (= D198), D339 (= D352)

5jlpA Crystal structure of mycobacterium avium serb2 in complex with serine at act domain
41% identity, 97% coverage: 5:396/404 of query aligns to 5:383/396 of 5jlpA

query
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5jlpA

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T

I

V

T

T

G

G

V

V

D
|
D

R
x
Q

P

P

G

G

L

V

T

T

A

A

A

T

I

L

T

F

G

E

V

V

L

L

A

S

Q

R

G

H

G

G

V

V

N

E

I

L

L

L

D

N

I

V

G

E

Q

Q

A

V

V

V

I

I

H

R

D

H

T

R

L

L

S

T

F

L

G

G

I

V

L

L

V

V

E

C

I

C

P

P

-

A

D

D

T

V

E

A

Q

D

G

G

S

P

S

A

V

L

L

R

K

H

D

D

I

V

L

E

F

A

K

A

A

I

Y

R

E

K

L

V

G

G

Q

L

Q

D

V

V

R

S

F

I

T

E

P

-

V

-

S

R

E

S

E

D

D

D

Y

V

Q

P

L

I

W

I

V

-

G

-

A

-

Q

R

G

E

K

P

K

S

R

T

H

H

I

T

V

I

T

F

L

V

L

L

T

G

R

R

K

P

V

I

T

T

A

A

E

A

Q

A

L

F

Q

G

R

A

V

V

S

A

S

R

I

E

T

V

A

A

K

A

Y

L

G

G

L

V

N

N

I

I

D

D

H

L

I

I

D

R

R

G

L

V

S

S

G

-

R

-

M

-

P

-

L

-

D

D

T

Y

P

P

A

V

D

I

K

G

G

-

K

-

G

-

C

-

I

L

E

E

F

L

S

R

V

V

R

S

G

V

E

P

A

P

A

G

D

A

P

D

Q

G

A

A

L

L

R

R

A

T

E

A

F

L

L

N

S

R

V

V

A

S

Q

S

E

E

L

E

N

H

V

V

D

D

I

V

A

A

F

V

Q

E

E

D

D

Y

S

T

L

L

F

E

R

R

N

A

R

K

R

R

L

L

A

I

V

V

F

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

L

I

V

E

Q

A

G

E

E

V

V

I

I

D

E

E

M

L

L

A

A

K

A

A

K

A

A

G

G

V

A

G

E

E

G

K

Q

V

V

S

A

E

A

I

I

T

T

E

D

R

A

A

A

M

M

A

R

G

G

E

E

L

L

D

D

F

F

R

A

A

Q

S

S

F

L

K

Q

E

Q

R

R

L

V

A

A

L

T

L

L

K

A

G

G

L

L

D

P

V

A

S

T

V

V

L

I

D

D

S

E

I

V

G

A

A

G

S

Q

L

L

R

E

L

L

T

M

E

P

G

G

A

A

E

R

T

T

L

T

F

L

A

R

E

T

L

L

K

R

R

R

L

L

G

G

Y

Y

K

A

T

C

A

G

I

V

L

V

S

S

G
|
G

G

G

F

F

T

R

Y

R

F

I

A

I

K

E

Q

P

L

L

Q

A

A

E

K

E

L

L

G

M

I

L

D

D

Y

Y

V

V

F

A

A

A

N

N

E

E

L

L

E

E

V

I

V

V

D

D

G

G

K

T

V

L

T

T

G

G

V

R

A

V

V

V

E

G

P

P

I

I

V

I

D

D

A

R

Q

A

R

G

K
|
K

A

A

D

T

L

A

L

L

R

R

E

E

L

F

A

A

H

Q

K

R

E

A

G

G

L

V

R

P

L

M

E

A

Q

Q

T

T

I

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

I

P

D

M

M

L

L

A

A

I

A

A

A

G

G

L

L

G

G

V

I

A

A

F

F

R

N

A

A

K

K

P

P

L

A

V

L

K

R

Q

E

S

V

A

A

K

D

Q

A

A

S

I

L

S

S

T

H

L

P

G

Y

L

L

D

D

G

T

V

V

L

L

Y

F

L

L

G

G

active site: D183 (= D196), V184 (≠ M197), D185 (= D198), G272 (= G285), K316 (= K329), D343 (= D356)
binding magnesium ion: D183 (= D196), D339 (= D352)
binding serine: D13 (= D13), Q14 (≠ R14)

A0QJI1 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Mycobacterium avium (strain 104) (see paper)
41% identity, 97% coverage: 5:396/404 of query aligns to 9:387/411 of A0QJI1

query
sites
A0QJI1

V

V

L

L

I

I

N

T

I

V

T

T

G

G

V

V

D

D

R

Q

P

P

G

G

L

V

T

T

A

A

A

T

I

L

T

F

G

E

V

V

L

L

A

S

Q

G

G

H

G

G

V

V

N

E

I

L

L

L

D

N

I

V

G

E

Q

Q

A

V

V

V

I

I

H

R

D

H

T

R

L

L

S

T

F

L

G

G

I

V

L

L

V

V

E

C

I

C

P

P

-

A

D

D

T

V

E

A

Q

D

G

G

S

P

S

A

V

L

L

R

K

H

D

D

I

V

L

E

F

A

K

A

A

I

Y

R

E

K

L

V

G

G

Q

L

Q

D

V

V

R

S

F

I

T

E

P

-

V

-

S

R

E

S

E

D

D

D

Y

V

Q

P

L

I

W

I

V

-

G

-

A

-

Q

R

G

E

K

P

K

S

R

T

H

H

I

T

V

I

T

F

L

V

L

L

T

G

R

R

K

P

V

I

T

T

A

A

E

A

Q

A

L

F

Q

G

R

A

V

V

S

A

S

R

I

E

T

V

A

A

K

A

Y

L

G

G

L

V

N

N

I

I

D

D

H

L

I

I

D

R

R

G

L

V

S

S

G

-

R

-

M

-

P

-

L

-

D

D

T

Y

P

P

A

V

D

I

K

G

G

-

K

-

G

-

C

-

I

L

E

E

F

L

S

R

V

V

R

S

G

V

E

P

A

P

A

G

D

A

P

D

Q

G

A

A

L

L

R

R

A

T

E

A

F

L

L

N

S

R

V

V

A

S

Q

S

E

E

L

E

N

H

V

V

D

D

I

V

A

A

F

V

Q

E

E

D

D

Y

S

T

L

L

F

E

R

R

N

A

R

K

R

R

L

L

A

I

V

V

F

F

D
|
D

M

V

D
|
D

S

S

T

T

L

L

I

V

E

Q

A

G

E

E

V

V

I

I

D

E

E

M

L

L

A

A

K

A

A

K

A

A

G

G

V

A

G

E

E

G

K

Q

V

V

S

A

E

A

I

I

T

T

E

D

R

A

A

A

M

M

A

R

G

G

E

E

L

L

D

D

F

F

R

A

A

Q

S

S

F

L

K

Q

E

Q

R

R

L

V

A

A

L

T

L

L

K

A

G

G

L

L

D

P

V

A

S

T

V

V

L

I

D

D

S

E

I

V

G

A

A

G

S

Q

L

L

R

E

L

L

T

M

E

P

G

G

A

A

E

R

T

T

L

T

F

L

A

R

E

T

L

L

K

R

R

R

L

L

G

G

Y

Y

K

A

T

C

A

G

I

V

L

V

S

S

G

G

F

F

T

R

Y

R

F

I

A

I

K

E

Q

P

L

L

Q

A

A

E

K

E

L

L

G

M

I

L

D

D

Y

Y

V

V

F

A

A

A

N

N

E

E

L

L

E

E

V

I

V

V

D

D

G

G

K

T

V

L

T

T

G

G

V

R

A

V

V

V

E

G

P

P

I

I

V

I

D

D

A

R

Q

A

R

G

K

K

A

A

D

T

L

A

L

L

R

R

E

E

L

F

A

A

H

Q

K

R

E

A

G

G

L

V

R

P

L

M

E

A

Q

Q

T

T

I

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D

D

L

I

P

D

M

M

L

L

A

A

I

A

A

A

G

G

L

L

G

G

V

I

A

A

F

F

R

N

A

A

K

K

P

P

L

A

V

L

K

R

Q

E

S

V

A

A

K

D

Q

A

A

S

I

L

S

S

T

H

L

P

G

Y

L

L

D

D

G

T

V

V

L

L

Y

F

L

L

G

G

D187 (= D196) binding
D189 (= D198) binding
D343 (= D352) binding

1f5sA Crystal structure of phosphoserine phosphatase from methanococcus jannaschii (see paper)
44% identity, 51% coverage: 188:392/404 of query aligns to 2:206/210 of 1f5sA

query
sites
1f5sA

R

K

N

K

R

K

L

L

A

I

V

L

F

F

D
|
D

M
x
F

D
|
D

S

S

T

T

L

L

I

V

E

N

A

N

E

E

V

T

I

I

D

D

E

E

L

I

A

A

K

R

A

E

A

A

G

G

V

V

G

E

E

E

K

E

V

V

S

K

E

K

I

I

T

T

E

K

R

E

A

A

M

M

A

E

G

G

E

K

L

L

D

N

F

F

R

E

A

Q

S

S

F

L

K

R

E

K

R

R

L

V

A

S

L

L

K

K

G

D

L

L

D

P

V

I

S

E

V

K

L

V

D

E

S

K

I

A

G

I

A

K

S

R

L

I

R

T

L

P

T

T

E

E

G

G

A

A

E

E

T

E

L

T

F

I

A

K

E

E

L

L

K

K

R

N

L

R

G

G

Y

Y

K

V

T

V

A

A

I

V

L

V

S
|
S

G
|
G

G

G

F

F

T

D

Y

I

F

A

A

V

K

N

Q

K

L

I

Q

K

A

E

K

K

L

L

G

G

I

L

D

D

Y

Y

V

A

F

F

A

A

N

N

E

R

L

L

E

I

V

V

V

K

D

D

G

G

K

K

V

L

T

T

G

G

V

D

A

V

V

E

E

G

P

E

I

V

V

L

D

K

A

E

Q

N

R

A

K
|
K

A

G

D

E

L

I

L

L

R

E

E

K

L

I

A

A

H

K

K

I

E

E

G

G

L

I

R

N

L

L

E

E

Q

D

T

T

I

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

I

P

S

M

M

L

F

A

K

I

K

A

A

G

G

L

L

G

K

V

I

A

A

F

F

R

C

A

A

K

K

P

P

L

I

V

L

K

K

Q

E

S

K

A

A

K

D

Q

I

A

C

I

I

S

E

T

K

L

R

G

D

L

L

D

R

G

E

V

I

L

L

active site: D10 (= D196), F11 (≠ M197), D12 (= D198), G99 (= G285), K143 (= K329), D170 (= D356)
binding magnesium ion: D10 (= D196), D12 (= D198), D166 (= D352)
binding phosphate ion: D10 (= D196), F11 (≠ M197), D12 (= D198), S98 (= S284), G99 (= G285), K143 (= K329)

Q58989 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see 3 papers)
43% identity, 51% coverage: 186:392/404 of query aligns to 1:207/211 of Q58989

query
sites
Q58989

F

M

R

E

R

K

N

K

R

K

L

L

A

I

V

L

F

F

D
|
D

M

F

D
|
D

S

S

T

T

L

L

I

V

E

N

A

N

E
|
E

V

T

I

I

D

D

E

E

L

I

A

A

K

R

A

E

A

A

G

G

V

V

G

E

E

E

K

E

V

V

S

K

E

K

I

I

T

T

E

K

R

E

A

A

M

M

A

E

G

G

E

K

L

L

D

N

F

F

R

E

A

Q

S

S

F

L

K

R

E

K

R
|
R

L

V

A

S

L

L

K

K

G

D

L

L

D

P

V

I

S

E

V

K

L

V

D

E

S

K

I

A

G

I

A

K

S

R

L

I

R

T

L

P

T

T

E

E

G

G

A

A

E

E

T

E

L

T

F

I

A

K

E

E

L

L

K

K

R

N

L

R

G

G

Y

Y

K

V

T

V

A

A

I

V

L

V

S
|
S

G
|
G

G

G

F

F

T

D

Y

I

F

A

A

V

K

N

Q

K

L

I

Q

K

A

E

K

K

L

L

G

G

I

L

D

D

Y

Y

V

A

F

F

A

A

N

N

E

R

L

L

E

I

V

V

V

K

D

D

G

G

K

K

V

L

T

T

G

G

V

D

A

V

V

E

E

G

P

E

I

V

V

L

D

K

A

E

Q

N

R

A

K
|
K

A

G

D

E

L

I

L

L

R

E

E

K

L

I

A

A

H

K

K

I

E

E

G

G

L

I

R

N

L

L

E

E

Q

D

T

T

I

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N
|
N

D

D

L

I

P

S

M

M

L

F

A

K

I

K

A

A

G

G

L

L

G

K

V

I

A

A

F

F

R

C

A

A

K

K

P

P

L

I

V

L

K

K

Q

E

S

K

A

A

K

D

Q

I

A

C

I

I

S

E

T

K

L

R

G

D

L

L

D

R

G

E

V

I

L

L

D11 (= D196) active site, Nucleophile; binding ; mutation to N: Loss of activity.
D13 (= D198) active site, Proton donor; binding
E20 (= E205) binding
R56 (= R241) binding
SG 99:100 (= SG 284:285) binding
K144 (= K329) binding
D167 (= D352) binding
N170 (= N355) binding

1l7nA Transition state analogue of phosphoserine phosphatase (aluminum fluoride complex) (see paper)
44% identity, 51% coverage: 188:392/404 of query aligns to 1:205/209 of 1l7nA

query
sites
1l7nA

R

K

N

K

R

K

L

L

A

I

V

L

F

F

D
|
D

M
x
F

D
|
D

S

S

T

T

L

L

I

V

E

N

A

N

E

E

V

T

I

I

D

D

E

E

L

I

A

A

K

R

A

E

A

A

G

G

V

V

G

E

E

E

K

E

V

V

S

K

E

K

I

I

T

T

E

K

R

E

A

A

M

M

A

E

G

G

E

K

L

L

D

N

F

F

R

E

A

Q

S

S

F

L

K

R

E

K

R

R

L

V

A

S

L

L

K

K

G

D

L

L

D

P

V

I

S

E

V

K

L

V

D

E

S

K

I

A

G

I

A

K

S

R

L

I

R

T

L

P

T

T

E

E

G

G

A

A

E

E

T

E

L

T

F

I

A

K

E

E

L

L

K

K

R

N

L

R

G

G

Y

Y

K

V

T

V

A

A

I

V

L

V

S
|
S

G
|
G

G

G

F

F

T

D

Y

I

F

A

A

V

K

N

Q

K

L

I

Q

K

A

E

K

K

L

L

G

G

I

L

D

D

Y

Y

V

A

F

F

A

A

N

N

E

R

L

L

E

I

V

V

V

K

D

D

G

G

K

K

V

L

T

T

G

G

V

D

A

V

V

E

E

G

P

E

I

V

V

L

D

K

A

E

Q

N

R

A

K
|
K

A

G

D

E

L

I

L

L

R

E

E

K

L

I

A

A

H

K

K

I

E

E

G

G

L

I

R

N

L

L

E

E

Q

D

T

T

I

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N
|
N

D
|
D

L

I

P

S

M

M

L

F

A

K

I

K

A

A

G

G

L

L

G

K

V

I

A

A

F

F

R

C

A

A

K

K

P

P

L

I

V

L

K

K

Q

E

S

K

A

A

K

D

Q

I

A

C

I

I

S

E

T

K

L

R

G

D

L

L

D

R

G

E

V

I

L

L

active site: D9 (= D196), F10 (≠ M197), D11 (= D198), G98 (= G285), K142 (= K329), D169 (= D356)
binding aluminum fluoride: D9 (= D196), F10 (≠ M197), D11 (= D198), S97 (= S284), K142 (= K329)
binding tetrafluoroaluminate ion: D9 (= D196), F10 (≠ M197), D11 (= D198), S97 (= S284), G98 (= G285), K142 (= K329), N168 (= N355)
binding magnesium ion: D9 (= D196), D11 (= D198), D165 (= D352)

7qplA Crystal structure of phosphoserine phosphatase (serb) from brucella melitensis in complex with phosphate and magnesium
44% identity, 56% coverage: 176:401/404 of query aligns to 68:292/295 of 7qplA

query
sites
7qplA

V

I

D

D

I

V

A

V

F

V

Q

Q

E

E

D

Q

S

E

L

-

F

-

R

R

N

R

R

K

L

I

A

L

V

I

F

A

D
|
D

M

M

D
|
D

S

S

T

T

L

M

I

I

E

G

A

Q

E

E

V

C

I

I

D

D

E

E

L

L

A

A

K

E

A

E

A

A

G

G

V

L

G

R

E

D

K

H

V

V

S

A

E

A

I

I

T

T

E

A

R

R

A

A

M

M

A

N

G

G

E

E

L

I

D

A

F

F

R

E

A

P

S

A

F

L

K

R

E

E

R

R

L

V

A

A

L

L

K

K

G

G

L

L

D

P

V

L

S

S

V

V

L

I

D

D

S

K

-

V

I

I

G

S

A

T

S

R

L

I

R

T

L

L

T

T

E

P

G

G

A

G

E

P

T

Q

L

L

F

V

A

R

E

T

L

M

K

R

R

K

L

H

G

G

Y

A

K

Y

T

T

A

A

I

L

L

V

S

S

G

G

F

F

T

T

Y

S

F

F

A

T

K

R

Q

R

L

I

Q

A

A

E

K

M

L

I

G

G

I

F

D

N

Y

E

V

E

F

R

A

A

N

N

E

R

L

L

E

-

V

I

V

D

D

D

G

G

-

T

K

R

V

L

T

T

G

G

V

T

A

V

V

A

E

E

P

P

I

I

V

L

D

G

A

R

Q

E

R

A

K

K

A

V

D

E

L

K

L

L

R

V

E

E

L

I

A

A

H

E

K

R

E

V

G

G

L

L

R

T

L

P

E

E

Q

D

T

A

I

I

A

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D

D

L

L

P

G

M

M

L

I

A

Q

I

L

A

A

G

G

L

T

G

G

V

V

A

A

F

L

R

H

A

A

K

K

P

P

L

A

V

V

K

A

Q

A

S

Q

A

A

K

K

Q

M

A

R

I

I

S

D

T

H

L

G

G

D

L

L

D

T

G

A

V

L

L

L

Y

Y

L

I

L

Q

G

G

F

Y

R

R

D

K

R

A

D

D

binding magnesium ion: D86 (= D196), D88 (= D198), D243 (= D352)

1l7pA Substrate bound phosphoserine phosphatase complex structure (see paper)
44% identity, 50% coverage: 190:392/404 of query aligns to 2:204/208 of 1l7pA

query
sites
1l7pA

R

K

L

L

A

I

V

L

F

F

D
x
N

M
x
F

D
|
D

S

S

T

T

L

L

I

V

E

N

A

N

E
|
E

V

T

I

I

D

D

E

E

L

I

A

A

K

R

A

E

A

A

G

G

V

V

G

E

E

E

K

E

V

V

S

K

E

K

I

I

T

T

E

K

R

E

A

A

M
|
M

A

E

G

G

E

K

L

L

D

N

F
|
F

R

E

A

Q

S

S

F

L

K

R

E

K

R
|
R

L

V

A

S

L

L

K

K

G

D

L

L

D

P

V

I

S

E

V

K

L

V

D

E

S

K

I

A

G

I

A

K

S

R

L

I

R

T

L

P

T

T

E

E

G

G

A

A

E

E

T

E

L

T

F

I

A

K

E

E

L

L

K

K

R

N

L

R

G

G

Y

Y

K

V

T

V

A

A

I

V

L

V

S
|
S

G
|
G

G

G

F

F

T

D

Y

I

F

A

A

V

K

N

Q

K

L

I

Q

K

A

E

K

K

L

L

G

G

I

L

D

D

Y

Y

V

A

F

F

A

A

N

N

E

R

L

L

E

I

V

V

V

K

D

D

G

G

K

K

V

L

T

T

G

G

V

D

A

V

V

E

E

G

P

E

I

V

V

L

D

K

A

E

Q

N

R

A

K
|
K

A

G

D

E

L

I

L

L

R

E

E

K

L

I

A

A

H

K

K

I

E

E

G

G

L

I

R

N

L

L

E

E

Q

D

T

T

I

V

A

A

V

V

G

G

D

D

G

G

A

A

N

N

D
|
D

L

I

P

S

M

M

L

F

A

K

I

K

A

A

G

G

L

L

G

K

V

I

A

A

F

F

R

C

A

A

K

K

P

P

L

I

V

L

K

K

Q

E

S

K

A

A

K

D

Q

I

A

C

I

I

S

E

T

K

L

R

G

D

L

L

D

R

G

E

V

I

L

L

active site: N8 (≠ D196), F9 (≠ M197), D10 (= D198), G97 (= G285), K141 (= K329), D168 (= D356)
binding phosphoserine: N8 (≠ D196), F9 (≠ M197), D10 (= D198), E17 (= E205), M40 (= M228), F46 (= F234), R53 (= R241), S96 (= S284), G97 (= G285), K141 (= K329)

1l7oA Crystal structure of phosphoserine phosphatase in apo form (see paper)
42% identity, 50% coverage: 190:392/404 of query aligns to 2:196/200 of 1l7oA

query
sites
1l7oA

R

K

L

L

A

I

V

L

F

F

D

N

M

F

D

D

S

S

T

T

L

L

I

V

E

N

A

N

E

E

V

T

I

I

D

D

E

E

L

I

A

A

K

R

A

E

A

A

G

G

V

V

G

E

E

E

K

E

V

V

S

K

E

K

I

I

T

T

E

-

R

-

A

-

M

-

A

-

G

-

E

-

L

-

D

N

F

F

R

E

A

Q

S

S

F

L

K

R

E

K

R

R

L

V

A

S

L

L

K

K

G

D

L

L

D

P

V

I

S

E

V

K

L

V

D

E

S

K

I

A

G

I

A

K

S

R

L

I

R

T

L

P

T

T

E

E

G

G

A

A

E

E

T

E

L

T

F

I

A

K

E

E

L

L

K

K

R

N

L

R

G

G

Y

Y

K

V

T

V

A

A

I

V

L

V

S

S

G

G

F

F

T

D

Y

I

F

A

A

V

K

N

Q

K

L

I

Q

K

A

E

K

K

L

L

G

G

I

L

D

D

Y

Y

V

A

F

F

A

A

N

N

E

R

L

L

E

I

V

V

V

K

D

D

G

G

K

K

V

L

T

T

G

G

V

D

A

V

V

E

E

G

P

E

I

V

V

L

D

K

A

E

Q

N

R

A

K

K

A

G

D

E

L

I

L

L

R

E

E

K

L

I

A

A

H

K

K

I

E

E

G

G

L

I

R

N

L

L

E

E

Q

D

T

T

I

V

A

A

V

V

G

G

D

D

G

G

A

A

N

N

D

D

L

I

P

S

M

M

L

F

A

K

I

K

A

A

G

G

L

L

G

K

V

I

A

A

F

F

R

C

A

A

K

K

P

P

L

I

V

L

K

K

Q

E

S

K

A

A

K

D

Q

I

A
x
C

I

I

S
x
E

T

K

L

R

G

D

L

L

D

R

G

E

V

I

L

L

binding zinc ion: C186 (≠ A382), E188 (≠ S384)

3m1yC Crystal structure of a phosphoserine phosphatase (serb) from helicobacter pylori
37% identity, 48% coverage: 190:384/404 of query aligns to 3:196/208 of 3m1yC

query
sites
3m1yC

R

Q

R

K

L

L

A

A

V

V

F

F

D
|
D

M
x
F

D
|
D

S

S

T

T

L

L

I

V

E

N

A

A

E

E

V

T

I

I

D

E

E

S

L

L

A

A

K

R

A

A

A

W

G

G

V

V

G

F

E

D

K

E

V

V

S

K

E

T

I

I

T

T

E

L

R

K

A

A

M

M

A

N

G

G

E

T

L

-

D

D

F

F

R

H

A

K

S

S

F

L

K

I

E

L

R

R

L

V

A

S

L

K

L

L

K

K

G

N

L

M

D

P

V

L

S

K

V

L

L

A

D

K

S

E

I

V

G

C

A

E

S

S

L

L

R

P

L

L

T

F

E

E

G

G

A

A

E

L

T

E

L

L

F

V

A

S

E

A

L

L

K

K

R

E

L

K

G

N

Y

Y

K

K

T

V

A

V

I

C

L

F

S

S

G
|
G

G

G

F

F

T

D

Y

L

F

A

A

T

K

N

Q

H

L

Y

Q

R

A

D

K

L

L

L

G

H

I

L

D

D

Y

A

V

A

F

F

A

S

N

N

E

T

L

L

E

I

V

V

V

E

D

N

G

D

K

A

V

L

T

N

G

G

V

L

A

V

V

T

E

G

P

H

I

M

V

M

D

F

A

S

Q

H

R

S

K
|
K

A

G

D

E

L

M

L

L

R

L

E

V

L

L

A

Q

H

R

K

L

E

L

G

N

L

I

R

S

L

K

E

T

Q

N

T

T

I

L

A

V

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

L

P

S

M

M

L

F

A

K

I

H

A

A

G

H

L

I

G

K

V

I

A

A

F

F

R

N

A

A

K

K

P

E

L

V

V

L

K

K

Q

Q

S

H

A

A

K

T

Q

H

A

C

I

I

S

N

active site: D9 (= D196), F10 (≠ M197), D11 (= D198), G97 (= G285), K141 (= K329), D168 (= D356)
binding magnesium ion: D9 (= D196), D11 (= D198), D164 (= D352)

1l8oA Molecular basis for the local conformational rearrangement of human phosphoserine phosphatase (see paper)
35% identity, 47% coverage: 194:384/404 of query aligns to 15:211/222 of 1l8oA

query
sites
1l8oA

V

C

F

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

V

I

I

E

R

A

E

E

E

V

G

I

I

D

D

E

E

L

L

A

A

K

K

A

I

A

C

G

G

V

V

G

E

E

D

K

A

V

V

S

S

E

E

I

M

T

T

E

R

R

R

A

A

M

M

A

G

G

G

E

A

L

V

D

P

F

F

R

K

A

A

S

A

F

L

K

T

E

E

R

R

L

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

V

E

S

Q

V

V

L

Q

D

R

S

L

I

I

G

A

-

E

A

Q

S

P

L

P

R

H

L

L

T

T

E

P

G

G

A

I

E

R

T

E

L

L

F

V

A

S

E

R

L

L

K

Q

R

E

L

R

G

N

Y

V

K

Q

T

V

A

F

I

L

L

I

S
|
S

G
|
G

G

G

F

F

T

R

Y

S

F

I

A

V

K

E

Q

H

L

V

Q

A

A

S

K

K

L

L

G

N

I

I

D

P

-

A

-

T

Y

N

V

V

F

F

A

A

N

N

E

R

L

L

E

K

V

F

-

Y

V

F

D

N

G

G

K

E

V

Y

T

A

G

G

V

F

-

D

A

E

V

T

E

Q

P

P

I

T

V

A

D

E

A

S

Q

G

R

G

K
|
K

A

G

D

K

L

V

L

I

R

K

E

F

L

L

A

-

H

-

K

K

E

E

G

K

L

F

R

H

L

F

E

K

Q

K

T

I

I

I

A

M

V

I

G

G

D

D

G
|
G

A

A

N
x
T

D
|
D

L

M

P

E

M

A

L

C

A

P

I

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

R

G

A

G

K

N

P

V

L

I

-
x
R

-

Q

V

V

K

K

Q

D

S

N

A

A

K

K

Q

W

A

Y

I

I

S

T

active site: D17 (= D196), V18 (≠ M197), D19 (= D198), G107 (= G285), K155 (= K329), D180 (= D356)
binding phosphate ion: D17 (= D196), D19 (= D198), S106 (= S284), K155 (= K329)
binding serine: G177 (= G353), T179 (≠ N355), R199 (vs. gap)

1l8lA Molecular basis for the local confomational rearrangement of human phosphoserine phosphatase (see paper)
35% identity, 47% coverage: 194:384/404 of query aligns to 15:211/222 of 1l8lA

query
sites
1l8lA

V

C

F

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

V

I

I

E

R

A

E

E

E

V

G

I

I

D

D

E

E

L

L

A

A

K

K

A

I

A

C

G

G

V

V

G

E

E

D

K

A

V

V

S

S

E

E

I

M

T

T

E

R

R

R

A

A

M

M

A

G

G

G

E

A

L

V

D

P

F

F

R

K

A

A

S

A

F

L

K

T

E

E

R

R

L

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

V

E

S

Q

V

V

L

Q

D

R

S

L

I

I

G

A

-

E

A

Q

S

P

L

P

R

H

L

L

T

T

E

P

G

G

A

I

E

R

T

E

L

L

F

V

A

S

E

R

L

L

K

Q

R

E

L

R

G

N

Y

V

K

Q

T

V

A

F

I

L

L

I

S

S

G
|
G

G

G

F

F

T

R

Y

S

F

I

A

V

K

E

Q

H

L

V

Q

A

A

S

K

K

L

L

G

N

I

I

D

P

-

A

-

T

Y

N

V

V

F

F

A

A

N

N

E

R

L

L

E

K

V

F

-

Y

V

F

D

N

G

G

K

E

V

Y

T

A

G

G

V

F

-

D

A

E

V

T

E

Q

P

P

I

T

V

A

D

E

A

S

Q

G

R

G

K
|
K

A

G

D

K

L

V

L

I

R

K

E

F

L

L

A

-

H

-

K

K

E

E

G

K

L

F

R

H

L

F

E

K

Q

K

T

I

I

I

A

M

V

I

G

G

D
|
D

G
|
G

A

A

N
x
T

D
|
D

L

M

P

E

M

A

L

C

A

P

I

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

R

G

A

G

K

N

P

V

L

I

-

R

-

Q

V

V

K

K

Q

D

S

N

A

A

K

K

Q

W

A

Y

I

I

S

T

active site: D17 (= D196), V18 (≠ M197), D19 (= D198), G107 (= G285), K155 (= K329), D180 (= D356)
binding d-2-amino-3-phosphono-propionic acid: D17 (= D196), D19 (= D198), G107 (= G285), K155 (= K329), D176 (= D352), G177 (= G353), T179 (≠ N355)

6hyjB Psph human phosphoserine phosphatase (see paper)
35% identity, 47% coverage: 194:384/404 of query aligns to 18:214/223 of 6hyjB

query
sites
6hyjB

V

C

F

F

D
|
D

M

V

D
|
D

S

S

T

T

L

V

I

I

E

R

A

E

E
|
E

V

G

I

I

D

D

E

E

L

L

A

A

K

K

A

I

A

C

G

G

V

V

G

E

E

D

K

A

V

V

S

S

E

E

I

M

T

T

E

R

R

R

A

A

M

M

A

G

G

G

E

A

L

V

D

P

F

F

R

K

A

A

S

A

F

L

K

T

E

E

R

R

L

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

V

E

S

Q

V

V

L

Q

D

R

S

L

I

I

G

A

-

E

A

Q

S

P

L

P

R

H

L

L

T

T

E

P

G

G

A

I

E

R

T

E

L

L

F

V

A

S

E

R

L

L

K

Q

R

E

L

R

G

N

Y

V

K

Q

T

V

A

F

I

L

L

I

S

S

G

G

F

F

T

R

Y

S

F

I

A

V

K

E

Q

H

L

V

Q

A

A

S

K

K

L

L

G

N

I

I

D

P

-

A

-

T

Y

N

V

V

F
|
F

A

A

N

N

E

R

L

L

E

K

V

F

-

Y

V

F

D

N

G

G

K

E

V

Y

T

A

G

G

V

F

-

D

A

E

V

T

E

Q

P

P

I

T

V

A

D

E

A

S

Q

G

R

G

K

K

A

G

D

K

L

V

L

I

R

K

E

L

L

L

A

-

H

-

K

K

E

E

G
x
K

L

F

R

H

L

F

E

K

Q

K

T

I

I

I

A

M

V

I

G

G

D
|
D

G

G

A

A

N

T

D

D

L

M

P

E

M

A

L

C

A

P

I

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

R

G

A

G

K

N

P

V

L

I

-
x
R

-

Q

V

V

K

K

Q

D

S

N

A

A

K

K

Q

W

A

Y

I

I

S

T

binding calcium ion: D20 (= D196), D22 (= D198), D179 (= D352)
binding phosphoserine: F131 (= F304), K168 (≠ G341)
binding serine: E29 (= E205), R202 (vs. gap)

P78330 Phosphoserine phosphatase; PSP; PSPase; L-3-phosphoserine phosphatase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Homo sapiens (Human) (see 4 papers)
35% identity, 47% coverage: 194:384/404 of query aligns to 18:214/225 of P78330

query
sites
P78330

V

C

F

F

D
|
D

M
x
V

D
|
D

S
|
S

T

T

L

V

I

I

E

R

A

E

E
|
E

V

G

I

I

D
|
D

E

E

L

L

A
|
A

K

K

A

I

A

C

G

G

V

V

G

E

E

D

K

A

V

V

S

S

E

E

I

M

T

T

E

R

R

R

A

A

M
|
M

A
x
G

G

G

E

A

L

V

D

P

F

F

R

K

A

A

S

A

F

L

K

T

E

E

R
|
R

L

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

V

E

S

Q

V

V

L

Q

D

R

S

L

I

I

G

A

-

E

A

Q

S

P

L

P

R

H

L

L

T

T

E

P

G

G

A

I

E

R

T

E

L

L

F

V

A

S

E

R

L

L

K

Q

R

E

L

R

G

N

Y

V

K

Q

T

V

A

F

I

L

L

I

S
|
S

G
|
G

F

F

T

R

Y

S

F

I

A

V

K

E

Q

H

L

V

Q

A

A

S

K

K

L

L

G

N

I

I

D

P

-

A

-

T

Y

N

V

V

F

F

A

A

N
|
N

E

R

L

L

E

K

V

F

-

Y

V

F

D

N

G

G

K

E

V

Y

T

A

G

G

V

F

-

D

A

E

V

T

E

Q

P

P

I

T

V

A

D

E

A

S

Q

G

R

G

K
|
K

A

G

D

K

L

V

L

I

R

K

E

L

L

L

A

-

H

-

K

K

E

E

G

K

L

F

R

H

L

F

E

K

Q

K

T

I

I

I

A

M

V

I

G

G

D
|
D

G

G

A

A

N
x
T

D

D

L

M

P

E

M

A

L

C

A

P

I

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

R

G

A

G

K

N

P

V

L

I

-
x
R

-

Q

V

V

K

K

Q

D

S

N

A

A

K

K

Q

W

A

Y

I

I

S

T

D20 (= D196) binding
DVD 20:22 (≠ DMD 196:198) binding
D22 (= D198) binding
S23 (= S199) mutation to A: Reduces L-phosphoserine phosphatase activity by about 50%.; mutation to T: Reduces L-phosphoserine phosphatase activity by about 80%.
E29 (= E205) mutation to D: Reduces L-phosphoserine phosphatase activity by about 95%.; mutation to Q: Loss of L-phosphoserine phosphatase activity.
D32 (= D208) to N: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894035
A35 (= A211) to T: in PSPHD; decreased L-phosphoserine phosphatase activity
M52 (= M228) binding ; to T: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894036
G53 (≠ A229) binding
R65 (= R241) mutation R->A,K: Loss of L-phosphoserine phosphatase activity.
SGG 109:111 (= SGG 284:286) binding ; binding
N133 (= N306) mutation to A: Reduces L-phosphoserine phosphatase activity by about 75%.
K158 (= K329) binding ; binding
D179 (= D352) binding
T182 (≠ N355) binding ; binding ; mutation to S: Reduces L-phosphoserine phosphatase activity by about 99%.; mutation to V: Reduces L-phosphoserine phosphatase activity by about 25%.
R202 (vs. gap) mutation to A: Reduces L-phosphoserine phosphatase activity by about 99%.; mutation to K: Reduces L-phosphoserine phosphatase activity by about 95%.

6hyyA Human phosphoserine phosphatase with serine and phosphate (see paper)
35% identity, 47% coverage: 194:384/404 of query aligns to 14:210/221 of 6hyyA

query
sites
6hyyA

V

C

F

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

V

I

I

E

R

A

E

E

E

V

G

I

I

D

D

E

E

L

L

A

A

K

K

A

I

A

C

G

G

V

V

G

E

E

D

K

A

V

V

S

S

E

E

I

M

T

T

E

R

R

R

A

A

M

M

A

G

G

G

E

A

L

V

D

P

F

F

R

K

A

A

S

A

F

L

K

T

E

E

R

R

L

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

V

E

S

Q

V

V

L

Q

D

R

S

L

I

I

G

A

-

E

A

Q

S

P

L

P

R

H

L

L

T

T

E

P

G

G

A

I

E

R

T

E

L

L

F

V

A

S

E

R

L

L

K

Q

R

E

L

R

G

N

Y

V

K

Q

T

V

A

F

I

L

L

I

S
|
S

G
|
G

G

G

F

F

T

R

Y

S

F

I

A

V

K

E

Q

H

L

V

Q

A

A

S

K

K

L

L

G

N

I

I

D

P

-

A

-

T

Y

N

V

V

F

F

A

A

N

N

E

R

L

L

E

K

V

F

-

Y

V

F

D

N

G

G

K

E

V

Y

T

A

G

G

V

F

-

D

A

E

V

T

E

Q

P

P

I

T

V

A

D

E

A

S

Q

G

R

G

K
|
K

A

G

D

K

L

V

L

I

R

K

E

L

L

L

A

-

H

-

K

K

E

E

G

K

L

F

R

H

L

F

E

K

Q

K

T

I

I

I

A

M

V

I

G

G

D
|
D

G

G

A

A

N

T

D

D

L

M

P

E

M

A

L

C

A

P

I

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

R

G

A

G

K

N

P

V

L

I

-

R

-

Q

V

V

K

K

Q

D

S

N

A

A

K

K

Q

W

A

Y

I

I

S

T

binding magnesium ion: D16 (= D196), D18 (= D198), D175 (= D352)
binding serine: D16 (= D196), V17 (≠ M197), D18 (= D198), S105 (= S284), G106 (= G285), K154 (= K329)

6q6jB Human phosphoserine phosphatase with substrate analogue homo-cysteic acid (see paper)
35% identity, 47% coverage: 194:384/404 of query aligns to 14:210/217 of 6q6jB

query
sites
6q6jB

V

C

F

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

V

I

I

E

R

A

E

E

E

V

G

I

I

D

D

E

E

L

L

A

A

K

K

A

I

A

C

G

G

V

V

G

E

E

D

K

A

V

V

S

S

E

E

I

M

T

T

E

R

R

R

A

A

M

M

A

G

G

G

E

A

L

V

D

P

F
|
F

R

K

A

A

S

A

F

L

K

T

E

E

R

R

L

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

V

E

S

Q

V

V

L

Q

D

R

S

L

I

I

G

A

-

E

A

Q

S

P

L

P

R

H

L

L

T

T

E

P

G

G

A

I

E

R

T

E

L

L

F

V

A

S

E

R

L

L

K

Q

R

E

L

R

G

N

Y

V

K

Q

T

V

A

F

I

L

L

I

S
|
S

G
|
G

F

F

T

R

Y

S

F

I

A

V

K

E

Q

H

L

V

Q

A

A

S

K

K

L

L

G

N

I

I

D

P

-

A

-

T

Y

N

V

V

F

F

A

A

N

N

E

R

L

L

E

K

V

F

-

Y

V

F

D

N

G

G

K

E

V

Y

T

A

G

G

V

F

-

D

A

E

V

T

E

Q

P

P

I

T

V

A

D

E

A

S

Q

G

R

G

K
|
K

A

G

D

K

L

V

L

I

R

K

E

L

L

L

A

-

H

-

K

K

E

E

G

K

L

F

R

H

L

F

E

K

Q

K

T

I

I

I

A

M

V

I

G

G

D
|
D

G

G

A

A

N
x
T

D

D

L

M

P

E

M

A

L

C

A

P

I

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

R

G

A

G

K

N

P

V

L

I

-

R

-

Q

V

V

K

K

Q

D

S

N

A

A

K

K

Q

W

A

Y

I

I

S

T

binding calcium ion: D16 (= D196), D18 (= D198), D175 (= D352)
binding (2~{S})-2-azanyl-4-sulfo-butanoic acid: D16 (= D196), V17 (≠ M197), D18 (= D198), F54 (= F234), S105 (= S284), G106 (= G285), G107 (= G286), K154 (= K329), T178 (≠ N355)

4ap9A Crystal structure of phosphoserine phosphatase from t.Onnurineus in complex with ndsb-201 (see paper)
27% identity, 44% coverage: 190:368/404 of query aligns to 9:174/200 of 4ap9A

query
sites
4ap9A

R

K

L

V

A

A

V

V

F

I

D
|
D

M
x
I

D
x
E

S

G

T

T

L

L

I

T

E

D

A

F

E

E

V

F

I

W

D

R

E

E

L

M

A

A

K
x
R

A
x
I

A
x
T

G

G

V

K

G

R

E

E

K

-

V

I

S

E

E

E

I

L

T

L

E

E

R

K

A

G

M
x
L

A

S

G

G

E

E

L

V

D

E

F
x
W

R

L

A

D

S

S

F

L

K

L

E

K

R

R

L

V

A

G

L

L

L

I

K

R

G

G

L

I

D

D

V

E

S

G

V

T

L

F

D

L

S

R

I

T

G

R

A

E

S

K

L

V

R

N

L

V

T

S

E

P

G

E

A

A

E

R

T

E

L

L

F

V

A

E

E

T

L

L

K

R

R

E

L

K

G

G

Y

F

K

K

T

V

A

V

I

L

L

I

S

S

G
|
G

G

S

F

F

T

E

Y

E

F

V

A

L

K

E

Q

P

L

F

Q

K

A

-

K

E

L

L

G

G

I

-

D

D

Y

E

V

F

F

M

A

A

N

N

E

R

L

A

E

I

V

F

V

E

D

D

G

G

K

K

V

F

T

Q

G

G

V

I

A

R

V

L

E

R

P

-

I

-

V

-

D

-

A

F

Q

R

R
x
D

K
|
K

A

G

D

E

L

F

L

L

R

K

E

R

L

F

A

-

H

-

K

R

E

D

G

G

L

F

R

-

L

-

E

-

Q

-

T

I

I

L

A

A

V

M

G

G

D

D

G

G

A
x
Y

N
x
A

D
|
D

L

A

P

K

M

M

L

F

A

E

I

R

A

A

G

D

L

M

G

G

V

I

A

A

active site: D15 (= D196), I16 (≠ M197), E17 (≠ D198), G103 (= G285), K141 (= K329), D162 (= D356)
binding 3-pyridinium-1-ylpropane-1-sulfonate: R31 (≠ K212), I32 (≠ A213), T33 (≠ A214), L46 (≠ M228), W52 (≠ F234), D140 (≠ R328), K141 (= K329), Y160 (≠ A354), A161 (≠ N355)

6iuyA Structure of dsgpdh of dunaliella salina (see paper)
30% identity, 41% coverage: 194:357/404 of query aligns to 18:180/585 of 6iuyA

query
sites
6iuyA

V

C

F

F

D
|
D

M

V

D
|
D

S

R

T

T

L

V

I

T

E

T

A

D

E

A

V

S

I

V

D

G

E

L

L

L

A

A

K

K

A

F

A

M

G

G

V

I

G

E

E

D

K

E

V

A

S

Q

E

S

I

L

T

T

E

E

R

Q

A

A

M

N

A

R

G

G

E

E

L

I

D

N

F

L

R

T

A

K

S

A

F

F

K

E

E

D

R

R

L

L

A

A

L

K

L

L

K

N

G

F

L

T

D

P

V

T

S

D

V

I

-

D

-

R

-

F

L

L

D

E

S

E

I

H

G

P

A

A

S

H

L

T

R

R

L

L

T

V

E

P

G

G

A

V

E

E

T

N

L

L

F

I

A

A

E

A

L

L

K

K

R

A

L

R

G

G

Y

V

K

E

T

V

A

F

I

L

L

I

S

S

G

G

F

F

T

R

Y

E

F

M

A

A

K

L

Q

P

L

I

Q

A

A

S

K

H

L

L

G

K

I

I

-

P

-

A

D

K

Y

N

V

V

F

F

A

C

N

N

E

T

L

M

E

S

V

W

-

Q

V

L

D

D

G

D

K

H

V

-

T

-

G

-

V

-

A

-

V

G

E

E

P

P

I

V

V

R

D

L

A

S

Q

H

R

F

K

K

A

S

D

R

L

A

L

I

R

E

E

R

L

I

A

R

H

R

K

K

E

-

G

-

L

Y

R

P

L

Y

E

N

Q

N

T

I

I

I

A

M

V

V

G

G

D
|
D

G

G

A

F

N

S

D

D

L

L

binding magnesium ion: D20 (= D196), D22 (= D198), D175 (= D352)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 233, 234, 235, 236, 268, 290, 321, 324, 349, 381, 382, 497, 529, 530, 532

Query Sequence

>AO353_13070 FitnessBrowser__pseudo3_N2E3:AO353_13070
LREIVLINITGVDRPGLTAAITGVLAQGGVNILDIGQAVIHDTLSFGILVEIPDTEQGSS
VLKDILFKAYELGQQVRFTPVSEEDYQLWVGAQGKKRHIVTLLTRKVTAEQLQRVSSITA
KYGLNIDHIDRLSGRMPLDTPADKGKGCIEFSVRGEAADPQALRAEFLSVAQELNVDIAF
QEDSLFRRNRRLAVFDMDSTLIEAEVIDELAKAAGVGEKVSEITERAMAGELDFRASFKE
RLALLKGLDVSVLDSIGASLRLTEGAETLFAELKRLGYKTAILSGGFTYFAKQLQAKLGI
DYVFANELEVVDGKVTGVAVEPIVDAQRKADLLRELAHKEGLRLEQTIAVGDGANDLPML
AIAGLGVAFRAKPLVKQSAKQAISTLGLDGVLYLLGFRDRDGQL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory