SitesBLAST

Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
25% identity, 84% coverage: 30:387/427 of query aligns to 28:388/824 of Q8GAI3

query
sites
Q8GAI3

V

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W66 (≠ G67) mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
H67 (≠ R68) mutation to A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.

5hxwA L-amino acid deaminase from proteus vulgaris (see paper)
27% identity, 58% coverage: 28:275/427 of query aligns to 17:261/433 of 5hxwA

query
sites
5hxwA

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active site: F58 (≠ N69), Q61 (≠ N74), A62 (= A75), Q240 (≠ Y255)
binding flavin-adenine dinucleotide: V22 (≠ I33), G23 (= G34), G25 (= G36), I26 (≠ F37), L27 (≠ T38), E46 (= E57), K47 (≠ A58), E53 (≠ G64), Q54 (≠ G65), S55 (= S66), R57 (= R68), F58 (≠ N69), Y59 (≠ V70), G60 (= G71), Q61 (≠ N74), A188 (= A206), A189 (≠ V207), G218 (≠ S235), G219 (≠ N236), W221 (≠ Y238), Q240 (≠ Y255), Q242 (≠ Y256)

Sites not aligning to the query:

active site: 284, 288
binding cetyl-trimethyl-ammonium: 291, 294, 310, 311, 317, 318, 320, 373, 379, 399, 400
binding flavin-adenine dinucleotide: 331, 371, 373, 398, 399, 400, 401, 402, 403

7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
23% identity, 83% coverage: 29:382/427 of query aligns to 3:344/363 of 7cyxA

query
sites
7cyxA

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binding flavin-adenine dinucleotide: I7 (= I33), G8 (= G34), G10 (= G36), V11 (≠ F37), I12 (≠ T38), V30 (≠ L56), E31 (= E57), K32 (≠ A58), E38 (≠ G64), A39 (≠ G65), S40 (= S66), A43 (≠ N69), G45 (= G71), L46 (= L72), V171 (= V207), G200 (≠ S235), G201 (≠ N236), W203 (≠ Y238), G298 (= G338), R300 (≠ A341), P301 (≠ F342), Y326 (≠ N364), R327 (≠ G365), N328 (≠ R366), G329 (= G367), I330 (≠ V368)

Q9UI17 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Homo sapiens (Human) (see 4 papers)
22% identity, 84% coverage: 28:387/427 of query aligns to 50:420/866 of Q9UI17

query
sites
Q9UI17

A

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A

P

V
|
V

T

T

R

S

L

L

E

K

-

A

R

R

Q

S

G

D

Q

G

R

T

W

W

S

D

V

V

Q

E

T

T

D

P

A

Q

G

G

S

S

V

M

M

R

A

A

E

N

Q

R

V

I

V

V

I

N

A

A

S

A

N

G

A

F

Y

W

T

A

E

R

-

E

-

V

G

G

D

K

W

M

T

I

E

G

L

L

R

E

R

H

N

P

F

L

F

I

P

P

G

V

Y

Q

Y

H

Y

Q

Q

Y

V

V

A

V

S

T

A

S

P

T

L

I

T
x
S

E

E

E

V

A

K

A

A

Q

L

L

K

-

R

-

E

-

L

-

P

I

V

L

L

P

R

G

D

G

L

Q

E

G

G

S

S

W

Y

D

-

T

-

R

-

Q

-

V

-

L

-

S

-

S

Y

I

L

R

R

R

Q

D

E

K

R

D

D

G

G

R

-

L

L

L

F

G

G

S

P

L

Y

G

E

N

S

G

Q

N

E

Q

K

-

M

-

K

-

V

K

Q

P

D

T

S

W

W

-

V

-

T

-

N

-

G

-

V

-

P

-

G

-

F

-

G

-

K

-

E

F

L

L

F

K

E

A

S

W

D

A

L

D

D

R

R

V

I

Q

M

Q

E

H

H

-

I

-

K

-

A

-

M

-

E

Y

M

F

V

P

P

Y

V

L

L

K

K

P

K

V

A

D

D

W

I

E

I

C

N

T

V

W

V

T

N

G

G

C

P

I

I

A

T

F

Y

T

S

P

P

D

D

H

-

L

I

M

L

R

P

L

M

F

V

E

G

P

P

A

H

P

Q

G

G

L

V

-

R

-

N

-

Y

-

W

V

V

A

A

V

I

T

-

G

G

Y
x
F

N

-

G
|
G

R
x
Y

G
|
G

V
x
I

T
x
I

T

H

G

A

T

G

V

G

V

V

G

G

K

K

A

Y

F

L

A

S

D

D

Y

W

L

I

C

L

N

H

G

G

N

E

P

P

CV 59:60 (≠ FT 37:38) binding
EK 80:81 (≠ EA 57:58) binding
87:95 (vs. 64:72, 33% identical) binding
H91 (≠ R68) modified: Tele-8alpha-FAD histidine
H109 (≠ L100) to R: in DMGDHD; shows 10 fold lower catalytic efficiency due to lower cofactor saturation and reduced thermal stability; dbSNP:rs121908331
V219 (= V207) binding
S279 (≠ T264) to P: in dbSNP:rs532964
F-GYGII 397:402 (≠ YNGRGVT 363:369) binding

Sites not aligning to the query:

530 A → G: in dbSNP:rs1805073
646 S → P: in dbSNP:rs1805074

4pabB Crystal structure of the precursor form of rat dmgdh complexed with tetrahydrofolate (see paper)
22% identity, 84% coverage: 28:387/427 of query aligns to 6:376/824 of 4pabB

query
sites
4pabB

A

A

D

E

V

T

C

V

V

I

I
|
I

G
|
G

A

G

G
|
G

F
x
C

T
x
V

G

G

L

V

S

S

A

L

A

A

V

Y

H

H

L

L

L

A

E

K

Q

A

G

G

-

M

K

R

S

D

V

V

C

V

V

L

L
|
L

E
|
E

A
x
K

K

S

R

E

C

L

G

T

Q

A

G
|
G

G
x
S

S
x
T

G

W

R
x
H

N
x
A

V
x
A

G
|
G

L
|
L

V

T

N
x
T

A

-

G

-

M

-

W

Y

I

F

P

H

P

P

D

-

E

-

I

-

E

-

A

-

G

-

F

-

G

-

E

-

A

-

I

-

G

G

S

I

Q

N

L

L

N

K

R

K

M

I

L

H

G

Y

A

D

A

S

P

I

A

K

L

L

V

Y

F

E

S

R

L

L

I

E

D

E

K

E

Y

T

N

G

I

Q

D

V

C

V

Q

G

L

F

R

H

R

Q

E

P

G

G

T

S

L

I

H

R

M

L

A

A

H

T

N

T

A

P

R

E

G

R

E

V

A

D

D
x
E

L

F

R

K

S

Y

R

Q

E

M

E

T

Q

R

W

T

K

N

R

W

R

H

G

A

A

T

P

E

V

Q

E

Y

L

I

T

E

G

P

A

E

A

K

C

I

E

H

Q

E

A

L

-

F

-

P

-

L

T

L

G

N

T

M

K

D

K

K

I

I

A

L

A

A

A

G

L

L

L

Y

D

N

R

P

R

G

A

D

G

G

T

H

L

I

N

D

P

P

M

Y

A

S

Y

L

T

T

S

M

G

A

L

L

A

A

K

T

A

G

A

A

A

R

D

K

L

Y

G

G

G

V

Q

L

L

L

F

K

D

Y

H

P

S

A

A

P

V
|
V

T

T

R

S

L

L

E

K

-

P

R

R

Q

P

G

D

Q

G

R

T

W

W

S

D

V

V

Q

E

T

T

D

P

A

Q

G

G

S

S

V

V

M

R

A

A

E

N

Q

R

V

I

V

V

I

N

A

A

S
x
A

N
x
G

A

F

Y
x
W

T

A

E

R

-

E

-

V

G

G

D

K

W

M

T

I

E

G

L

L

R

D

R

H

N

P

F

L

F

I

P

P

G

V

Y

Q

Y
x
H

Y

Q

Q
x
Y

V

V

A

V

S

T

A

S

P

T

L

I

T

P

E

E

E

V

A

K

A

A

Q

L

L

K

-

R

-

E

-

L

-

P

I

V

L

L

P

R

G

D

G

L

Q

E

G

G

S

S

W

Y

D

-

T

-

R

-

Q

-

V

-

L

-

S

-

S
x
Y

I

L

R

R

R

Q

D

E

K

R

D

D

G

G

R

-

L

L

L

F

G

G

S

P

L

Y

G

E

N

S

G

Q

N

E

Q

K

-

M

-

K

-

L

K

Q

P

A

T

S

W

W

-

V

-

A

-

H

-

G

-

V

-

P

-

G

-

F

-

G

-

K

-

E

F

L

L

F

K

E

A

S

W

D

A

L

D

D

R

R

V

I

Q

T

Q

E

H

H

-

V

-

E

-

A

-

M

-

E

Y

M

F

V

P

P

Y

V

L

L

K

K

P

K

V

A

D

D

W

I

E

I

C

N

T

I

W

V

T

N

G
|
G

C

P

I
|
I

A

T

F

Y

T

S

P

P

D

D

H

-

L

I

M

L

R

P

L

M

F

V

E

G

P

P

A

H

P

Q

G

G

L

V

-

R

-

N

-

Y

-

W

V

V

A

A

V

I

T

-

G

G

Y
x
F

N

-

G

G

R
x
Y

G
|
G

V
x
I

T
x
I

T

H

G

A

T

G

V

G

V

V

G

G

K

K

A

Y

F

L

A

S

D

D

Y

W

L

I

C

L

N

H

G

G

N

E

P

P

active site: T53 (≠ N74), E102 (≠ D137), H226 (≠ Y255), Y255 (≠ S287)
binding flavin-adenine dinucleotide: I11 (= I33), G12 (= G34), G14 (= G36), C15 (≠ F37), V16 (≠ T38), L35 (= L56), E36 (= E57), K37 (≠ A58), G43 (= G64), S44 (≠ G65), T45 (≠ S66), H47 (≠ R68), A48 (≠ N69), A49 (≠ V70), G50 (= G71), L51 (= L72), V175 (= V207), A204 (≠ S235), G205 (≠ N236), W207 (≠ Y238), H226 (≠ Y255), Y228 (≠ Q257), G326 (= G338), I328 (= I340), F353 (≠ Y363), Y355 (≠ R366), G356 (= G367), I357 (≠ V368), I358 (≠ T369)

Sites not aligning to the query:

active site: 536
binding (6s)-5,6,7,8-tetrahydrofolate: 523, 536, 538, 550, 612, 613, 632, 639, 680, 700

Q63342 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Rattus norvegicus (Rat) (see 2 papers)
22% identity, 84% coverage: 28:387/427 of query aligns to 43:413/857 of Q63342

query
sites
Q63342

A

A

D

E

V

T

C

V

V

I

I

I

G

G

A

G

G

G

F
x
C

T
x
V

G

G

L

V

S

S

A

L

A

A

V

Y

H

H

L

L

L

A

E

K

Q

A

G

G

-

M

K

R

S

D

V

V

C

V

V

L

L

L

E
|
E

A
x
K

K

S

R

E

C

L

G

T

Q

A

G
|
G

G
x
S

S
x
T

G
x
W

R
x
H

N
x
A

V
x
A

G
|
G

L
|
L

V

T

N

T

A

-

G

-

M

-

W

Y

I

F

P

H

P

P

D

-

E

-

I

-

E

-

A

-

G

-

F

-

G

-

E

-

A

-

I

-

G

G

S

I

Q

N

L

L

N

K

R

K

M

I

L

H

G

Y

A

D

A

S

P

I

A

K

L

L

V

Y

F

E

S

R

L

L

I

E

D

E

K

E

Y

T

N

G

I

Q

D

V

C

V

Q

G

L

F

R

H

R

Q

E

P

G

G

T

S

L

I

H

R

M

L

A

A

H

T

N

T

A

P

R

E

G

R

E

V

A

D

D

E

L

F

R

K

S

Y

R

Q

E

M

E

T

Q

R

W

T

K

N

R

W

R

H

G

A

A

T

P

E

V

Q

E

Y

L

I

T

E

G

P

A

E

A

K

C

I

E

H

Q

E

A

L

-

F

-

P

-

L

T

L

G

N

T

M

K

D

K

K

I

I

A

L

A

A

A

G

L

L

L

Y

D

N

R

P

R

G

A

D

G

G

T

H

L

I

N

D

P

P

M

Y

A

S

Y

L

T

T

S

M

G

A

L

L

A

A

K

T

A

G

A

A

A

R

D

K

L

Y

G

G

G

V

Q

L

L

L

F

K

D

Y

H

P

S

A

A

P

V
|
V

T

T

R

S

L

L

E

K

-

P

R

R

Q

P

G

D

Q

G

R

T

W

W

S

D

V

V

Q

E

T

T

D

P

A

Q

G

G

S

S

V

V

M

R

A

A

E

N

Q

R

V

I

V

V

I

N

A

A

S

A

N

G

A

F

Y
x
W

T

A

E

R

-

E

-

V

G

G

D

K

W

M

T

I

E

G

L

L

R

D

R

H

N

P

F

L

F

I

P

P

G

V

Y

Q

Y

H

Y

Q

Q

Y

V

V

A

V

S

T

A

S

P

T

L

I

T

P

E

E

E

V

A

K

A

A

Q

L

L

K

-

R

-

E

-

L

-

P

I

V

L

L

P

R

G

D

G

L

Q

E

G

G

S

S

W

Y

D

Y

T

L

R

R

Q

Q

V

-

L

-

S

-

I

-

R

-

D

E

K

R

D

D

G

G

R

-

L

L

L

F

G

G

S

P

L

Y

G

E

N

S

G

Q

N

E

Q

K

-

M

-

K

-

L

K

Q

P

A

T

S

W

W

-

V

-

A

-

H

-

G

-

V

-

P

-

G

-

F

-

G

-

K

-

E

F

L

L

F

K

E

A

S

W

D

A

L

D

D

R

R

V

I

Q

T

Q

E

H

H

-

V

-

E

-

A

-

M

-

E

Y

M

F

V

P

P

Y

V

L

L

K

K

P

K

V

A

D

D

W

I

E

I

C

N

T

I

W

V

T

N

G

G

C

P

I

I

A

T

F

Y

T

S

P

P

D

D

H

-

L

I

M

L

R

P

L

M

F

V

E

G

P

P

A

H

P

Q

G

G

L

V

-

R

-

N

-

Y

-

W

V

V

A

A

V

I

T

-

G

G

Y
x
F

N

-

G
|
G

R
x
Y

G
|
G

V
x
I

T
x
I

T

H

G

A

T

G

V

G

V

V

G

G

K

K

A

Y

F

L

A

S

D

D

Y

W

L

I

C

L

N

H

G

G

N

E

P

P

CV 52:53 (≠ FT 37:38) binding
EK 73:74 (≠ EA 57:58) binding
80:88 (vs. 64:72, 33% identical) binding
H84 (≠ R68) modified: Tele-8alpha-FAD histidine
V212 (= V207) binding
W244 (≠ Y238) binding
F-GYGII 390:395 (≠ YNGRGVT 363:369) binding

Sites not aligning to the query:

573:575 binding
669 binding
676:678 binding
737 binding

1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
32% identity, 17% coverage: 181:252/427 of query aligns to 148:221/364 of 1ng3A

query
sites
1ng3A

L

V

N

E

P

P

M

Y

A

F

Y

V

T

C

S

K

G

A

L

Y

A

V

K

K

A

A

A

K

D

M

L

L

G

G

G

A

Q

E

L

I

F

F

D

E

H

H

S

T

A

P

V
|
V

T

L

R

H

L

V

E

E

R

R

Q

D

G

G

Q

E

R

A

W

L

S

F

V

I

Q

K

T

T

D

P

A

S

G

G

S

D

V

V

M

W

A

A

E

N

Q

H

V

V

I

V

A

A

S
|
S

N
x
G

A

V

Y
x
W

T

S

E

G

G

M

D
x
F

W

F

T

K

E

Q

L

L

R

G

R

L

N

N

-

N

-

A

F

F

F

L

P

P

binding flavin-adenine dinucleotide: V174 (= V207), S202 (= S235), G203 (≠ N236), W205 (≠ Y238), F209 (≠ D242)

Sites not aligning to the query:

active site: 47, 48, 49
binding acetylamino-acetic acid: 246, 302, 329
binding flavin-adenine dinucleotide: 11, 13, 15, 33, 34, 35, 41, 42, 43, 46, 47, 48, 49, 300, 302, 327, 329, 330, 331, 332
binding phosphate ion: 89, 254

3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
22% identity, 49% coverage: 45:252/427 of query aligns to 22:221/364 of 3if9A

query
sites
3if9A

H

Y

L

L

L

A

E

K

Q

E

G

N

K

K

S

N

V

T

C

A

V

L

L

F

E
|
E

A
x
S

K

G

R

T

C

M

G

G

Q

G

G

R

G
x
T

S
x
T

G

S

R

A

N
x
A

V
x
A

G
|
G

L
x
M

V

L

N

S

A

A

G

H

M

R

W

-

I

-

P

E

P

C

D

E

E

E

I

R

E

D

A

A

G

F

F

F

G

D

E

F

A

A

I

M

G

H

S

S

Q

Q

L

-

N

-

R

R

M

L

L

Y

G

K

A

G

A

L

P

G

A

E

L

E

V

L

F

Y

S

A

L

L

I

-

D

-

K

-

Y

S

N

G

I

V

D

D

C

I

Q

R

L

Q

R

H

R

N

E

G

G

G

T

M

L

F

H

K

M

L

A

A

H

F

N

S

A

E

R

E

G

D

E

V

A

L

D

Q

L

L

R

R

S

Q

R

M

E

D

-

L

-

D

-

S

-

V

Q

S

W

W

K

Y

R

S

R

K

G

E

A

E

P

V

V

L

E

E

L

-

T

-

G

-

A

-

C

-

E

K

Q

E

A

P

T

Y

G

A

T

S

K

G

K

D

I

I

A

F

A

G

A

A

L

S

L

F

D

I

R

Q

R

D

A

D

G

V

T

H

L

V

N

E

P

P

M

Y

A

F

Y

V

T

C

S

K

G

A

L

Y

A

V

K

K

A

A

A

K

D

M

L

L

G

G

G

A

Q

E

L

I

F

F

D

E

H

H

S

T

A
x
P

V
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V

T

L

R

H

L

V

E

E

R

R

Q

D

G

G

Q

E

R

A

W

L

S

F

V

I

Q

K

T

T

D

P

A

S

G

G

S

D

V

V

M

W

A

A

E

N

Q

H

V

V

I

V

A

A

S
|
S

N
x
G

A

V

Y
x
W

T

S

E

G

G

M

D
x
F

W

F

T

K

E

Q

L

L

R

G

R

L

N

N

-

N

-

A

F

F

F

L

P

P

active site: A47 (≠ V70), G48 (= G71), M49 (≠ L72)
binding flavin-adenine dinucleotide: E34 (= E57), S35 (≠ A58), T42 (≠ G65), T43 (≠ S66), A46 (≠ N69), A47 (≠ V70), G48 (= G71), M49 (≠ L72), P173 (≠ A206), V174 (= V207), S202 (= S235), G203 (≠ N236), W205 (≠ Y238), F209 (≠ D242)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 11, 13, 15, 300, 302, 327, 328, 329, 330, 331, 332
binding glycolic acid: 246, 302, 329

O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
32% identity, 17% coverage: 181:252/427 of query aligns to 148:221/369 of O31616

query
sites
O31616

L

V

N

E

P

P

M

Y

A

F

Y

V

T

C

S

K

G

A

L

Y

A

V

K

K

A

A

A

K

D

M

L

L

G

G

G

A

Q

E

L

I

F

F

D

E

H

H

S

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A

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V
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V

T

L

R

H

L

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E

E

R

R

Q

D

G

G

Q

E

R

A

W

L

S

F

V

I

Q

K

T

T

D

P

A

S

G

G

S

D

V

V

M

W

A

A

E

N

Q

H

V

V

I

V

A

A

S

S

N

G

A

V

Y

W

T

S

E

G

G

M

D

F

W

F

T

K

E

Q

L

L

R

G

R

L

N

N

-

N

-

A

F

F

F

L

P

P

V174 (= V207) binding

Sites not aligning to the query:

14:15 binding
34:35 binding
42:43 binding
47:49 binding
51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
244 H→A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
302 binding
327:333 binding
329 binding

S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
24% identity, 50% coverage: 41:252/427 of query aligns to 18:221/369 of S5FMM4

query
sites
S5FMM4

S

S

A

I

A

A

V

Y

H

H

L

L

L

A

E

K

Q

A

G

G

K

K

S

K

V

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C

A

V

V

L

F

E

E

A

S

K

G

R

E

C

V

G

G

Q

K

G

K

G

A

S

T

G

S

R

A

N

A

V

A

G

G

L

M

V

L

N
x
G

A

A

G

H

M
x
A

W

E

I

C

P

D

P

K

D

P

E

G

I

T

E

F

A

F

G

E

F

F

G

A

E

R

A

A

I

S

G

Q

S

K

Q

A

L

Y

N

K

R

R

M

L

L

T

G

G

A

-

P

-

A

-

L

-

V

-

F

-

S

E

L

L

I
x
K

D

D

K

I

Y

S

N

G

I

I

D

D

C

I

Q

R

L

R

R

H

R

D

E

G

G

G

T

I

L

L

H

K

M

L

A

A

H

F

N

S

A

-

R

-

G

-

E

-

A

-

D

-

L

-

R

E

S

S

R

D

E

R

E

E

Q

H

W

L

K

M

R

Q

R

M

G

G

A

A

-

L

-

D

P

S

V

V

E

E

L

W

L

L

T

E

G

A

A

D

A

E

C

V

E

Y

-

K

-

L

Q

E

A

P

T

N

G

A

T

G

K

K

K

G

I

I

A

L

A

G

A

A

L

N

L

F

D

I

R

R

R

D

A

D

G

V

T

H

L

V

N

E

P

P

M

A

A

A

Y

V

T

C

S

R

G

A

L

F

A

A

K

R

A

G

A

A

A

R

D

M

L

L

G

G

G

A

Q

D

L

V

F

F

D

E

H

Y

S

T

A

P

V

V

T

L

R

S

L

I

E

E

R

S

Q

E

G

A

Q

G

R

A

W

V

S

R

V

V

Q

T

T

S

D

A

A

S

G

G

S

T

V

A

M

E

A

A

E

E

Q

H

V

A

V

V

I

I

A

A

S
|
S

N

G

A

V

Y

W

T

S

E

G

G

A

D

L

W

F

T

K

E

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-

I

-

G

L

L

R

D

R

K

N

R

F

F

F

Y

P

P

G51 (≠ N74) mutation to S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
A54 (≠ M77) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
K81 (≠ I111) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
S202 (= S235) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.

Sites not aligning to the query:

332 I→V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
342 M→V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.

Query Sequence

>AO353_13335 FitnessBrowser__pseudo3_N2E3:AO353_13335
MPLREECLWEKLTPQRPDRAALKGEVTADVCVIGAGFTGLSAAVHLLEQGKSVCVLEAKR
CGQGGSGRNVGLVNAGMWIPPDEIEAGFGEAIGSQLNRMLGAAPALVFSLIDKYNIDCQL
RREGTLHMAHNARGEADLRSREEQWKRRGAPVELLTGAACEQATGTKKIAAALLDRRAGT
LNPMAYTSGLAKAAADLGGQLFDHSAVTRLERQGQRWSVQTDAGSVMAEQVVIASNAYTE
GDWTELRRNFFPGYYYQVASAPLTEEAAQLILPGGQGSWDTRQVLSSIRRDKDGRLLLGS
LGNGNQKPTWFLKAWADRVQQHYFPYLKPVDWECTWTGCIAFTPDHLMRLFEPAPGLVAV
TGYNGRGVTTGTVVGKAFADYLCNGNPQALPIPFAPMQPLAGVGLRSCLYEAGFSLYHAG
QCLRIVI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory