SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO353_13510 FitnessBrowser__pseudo3_N2E3:AO353_13510 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 14 hits to proteins with known functional sites (download)

A6VCX3 L-methionine sulfoximine/L-methionine sulfone acetyltransferase; Methionine derivative detoxifier A; MDDA; EC 2.3.1.- from Pseudomonas aeruginosa (strain PA7) (see paper)
72% identity, 97% coverage: 1:172/177 of query aligns to 1:172/172 of A6VCX3

query
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A6VCX3

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RPF 75:77 (= RPF 75:77) binding
EHS 85:87 (= EHS 85:87) binding

2j8mA Structure of p. Aeruginosa acetyltransferase pa4866 (see paper)
73% identity, 95% coverage: 5:172/177 of query aligns to 4:171/171 of 2j8mA

query
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2j8mA

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binding azide ion: W73 (= W74), R74 (= R75)

2j8rA Structure of p. Aeruginosa acetyltransferase pa4866 solved in complex with l-methionine sulfoximine (see paper)
73% identity, 95% coverage: 5:172/177 of query aligns to 3:170/170 of 2j8rA

query
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binding azide ion: W42 (= W44), Y66 (= Y68), S85 (= S87)
binding (2s)-2-amino-4-(methylsulfonimidoyl)butanoic acid: A27 (= A29), I28 (= I30), W29 (= W31), R73 (= R75), F75 (= F77), F78 (= F80), E83 (= E85)

Q8ZPD3 L-methionine sulfoximine/L-methionine sulfone acetyltransferase; L-amino acid N-acyltransferase; Methionine derivative detoxifier A; MDDA; EC 2.3.1.-; EC 2.3.1.- from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
63% identity, 94% coverage: 5:171/177 of query aligns to 3:168/171 of Q8ZPD3

query
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Q8ZPD3

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E82 (= E85) mutation to Q: Loss of acetyltransferase activity.
VYV 85:87 (= VYV 88:90) binding
GKGLGR 93:98 (≠ GNGLGP 96:101) binding
N124 (= N127) binding
S133 (≠ R136) binding

3dr8A Structure of ynca, a putative acetyltransferase from salmonella typhimurium with its cofactor acetyl-coa
63% identity, 94% coverage: 5:171/177 of query aligns to 5:170/173 of 3dr8A

query
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3dr8A

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binding acetyl coenzyme *a: A29 (= A29), H85 (= H86), S86 (= S87), V87 (= V88), Y88 (= Y89), V89 (= V90), Q94 (≠ R95), G95 (= G96), G97 (= G98), G99 (= G100), R100 (≠ P101), A127 (= A128), A128 (= A129), R131 (= R132), H133 (= H134), S135 (≠ R136)

4jwpA Crystal structure of ribosomal-protein-alanine n-acetyltransferase from brucella melitensis in complex with acetyl coa
58% identity, 93% coverage: 1:165/177 of query aligns to 2:165/165 of 4jwpA

query
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4jwpA

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binding acetyl coenzyme *a: A30 (= A29), V88 (= V88), Y89 (= Y89), V90 (= V90), R95 (= R95), G96 (= G96), G98 (= G98), G100 (= G100), R101 (≠ P101), G122 (≠ A122), A129 (= A129), S130 (= S130), A132 (≠ R132)
binding calcium ion: E110 (≠ R110), E113 (≠ D113)

2jlmF Structure of a putative acetyltransferase (aciad1637) from acinetobacter baylyi adp1 (see paper)
44% identity, 88% coverage: 16:171/177 of query aligns to 22:177/180 of 2jlmF

query
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2jlmF

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L

I

T

L

L

N

D

P

T

G

P

E

L

E

H

P

P

binding azide ion: T34 (= T28), A35 (= A29), Y95 (= Y89), I96 (≠ V90), D130 (≠ E124), N133 (= N127)

4mbuA Crystal structure of n-acetyltransferase from staphylococcus aureus mu50 (see paper)
43% identity, 92% coverage: 5:166/177 of query aligns to 5:165/165 of 4mbuA

query
sites
4mbuA

I

I

R

R

D

C

A

A

V

K

Y

K

A

E

D

D

L

L

P

N

A

A

I

I

R

L

D

A

I

I

Y

Y

N

N

D

D

A

A

V

I

L

I

N

N

T

T

A

A

I

V

W

Y

N

T

E

Y

Q

K

A

P

V

Q

D

T

L

I

A

D

N

E

R

R

Q

I

A

A

W

W

F

F

S

E

T

T

R

K

Q

Q

S

R

Q

N

G

H

Y

E

P

P

V

I

L

F

V

V

I

F

V

-

D

E

A

E

D

N

N

G

S

S

V

V

L

L

G

G

Y

F

A

A

S

T

F

F

G

G

D

S

W

F

R

R

P

P

F

W

D

P

G

A

F

Y

R

Q

H

Y

T

T

V

I

E

E

H

H

S

S

V

I

Y

Y

V

V

R

D

S

A

D

S

Q

A

R

R

G

G

N

K

G

G

L

I

G

A

P

S

Q

Q

L

L

M

L

D

Q

V

R

L

L

I

I

E

V

R

E

A

A

R

K

D

A

C

K

N

G

K

Y

H

R

V

T

M

L

V

V

A

A

A

G

I

I

E

D

S

A

A

S

N

N

A

E

A

A

S

S

I

I

R

K

L

L

H

H

E
x
Q

R

K

V

F

G

N

F

F

I
x
K

T
x
H

T

A

G

G

H

T

M

L

P

T

Q

N

V

V

G

G

T

Y

K

K

F

F

G

D

R

Y

W

W

L

L

D

D

L

L

T

A

F

F

M

Y

Q

E

L

L

I

D

L

L

N

K

binding phosphate ion: Q134 (≠ E135), K139 (≠ I140), H140 (≠ T141)

4jxrB Crystal structure of a gnat superfamily phosphinothricin acetyltransferase (pat) from sinorhizobium meliloti in complex with accoa
40% identity, 95% coverage: 1:168/177 of query aligns to 1:168/185 of 4jxrB

query
sites
4jxrB

M

M

T

T

Y

A

H

T

I

L

R

R

D

D

A

A

V

V

Y

A

A

A

D

D

L

L

P

R

A

S

I

I

R

T

D

E

I

I

Y

Y

N

R

D

E

A

S

V

V

L

L

N

N

T

G

T

V

A
|
A

I

T

W

Y

N

E

E

E

Q

T

A

P

V

P

D

S

L

E

A

A

N

E

R

M

Q

A

A

L

W

R

F

F

S

S

T

T

R

I

Q

T

S

G

Q

N

G

G

Y

Y

P

P

V

Y

L

V

V

V

I

A

V

L

D

D

A

E

D

R

N

G

S

A

V

V

L

I

G

G

Y

Y

A

A

S

Y

F

A

G

S

D

A

W

F

R

R

P

N

F

R

D

T

G

A

F

Y

R

R

H

F

T

L

V

V

E

E

H
x
D

S
|
S

V
x
I

Y
|
Y

V
x
L

R

S

S
x
P

D

E

Q

A

R
|
R

G
|
G

N

K

G

G

L

I

G
|
G

P

K

Q

A

L

L

M

L

D

S

V

E

L

L

I

V

E

G

R

R

A

C

R

T

D

A

C

L

N

G

K

F

H

R

V

Q

M

M

V

I

A

A

A

V

I

I

E

G

S

G

A

A

N
x
H

A

P

A
x
S

S
|
S

I

I

R

A

L

L

H
|
H

E

R

R

A

V

L

G

G

F

F

I

E

T

L

T

Q

G

G

H

L

M

M

P

K

Q

A

V

T

G

G

T

F

K

K

F

H

G

G

R

R

W

W

L

L

D

D

L

T

T

A

F

F

M

M

Q

Q

L

R

I

P

L

L

N

G

P

E

G

G

binding acetyl coenzyme *a: A29 (= A29), D86 (≠ H86), S87 (= S87), I88 (≠ V88), Y89 (= Y89), L90 (≠ V90), P92 (≠ S92), R95 (= R95), G96 (= G96), G100 (= G100), H127 (≠ N127), S129 (≠ A129), S130 (= S130), H134 (= H134)

5dwnA Crystal structure of phosphinothricin n-acetyltransferase from brucella ovis in complex with acetylcoa
37% identity, 93% coverage: 5:168/177 of query aligns to 6:170/181 of 5dwnA

query
sites
5dwnA

I

I

R

R

D

D

A

F

V

Q

Y

P

A

A

D

D

L

I

P

E

A

T

I

I

R

T

D

A

I

I

Y

Y

N

T

D

Q

A

A

V

V

L

L

N

T

T

G

T

T

A

G

I

S

W

Y

N

E

E

I

Q

E

A

P

V

P

D

T

L

M

A

D

N

E

R

M

Q

A

A

K

W

R

F

F

S

A

T

A

R

F

Q

A

S

D

Q

Q

G

G

Y

F

P

P

V

I

L

L

V

V

I

-

V

A

D

E

A

A

D

D

N

G

S

R

V

V

L

L

G

G

Y

Y

A

A

S

Y

F

A

G

S

D

Y

W

F

R

R

P

V

F

R

D

P

G

A

F

Y

R

R

H

W

T

L

V

A

E

E

H

D

S
|
S

V
x
I

Y
|
Y

V
x
I

R

A

S

P

D

D

Q

A

R
x
K

G
|
G

N

Q

G
|
G

L

I

G
|
G

P
x
K

Q

L

L

L

M

L

D

R

V

E

L

L

I

I

E

A

R

R

A

I

R

S

D

A

C

L

N

G

K

F

H

R

V

Q

M

L

V

L

A

A

A

V

I

I

E

G

S

D

A

G

-

E

-

H

N

N

A

I

A
x
G

S
|
S

I

V

R
x
K

L
|
L

H
|
H

E

E

R

S

V

L

G

G

F

F

I

T

T

H

T

C

G

G

H

R

M

I

P

E

Q

G

V

S

G

G

T

F

K

K

F

H

G

G

R

R

W

W

L

L

D

D

L

T

T

V

F

L

M

M

Q

Q

L

L

I

P

L

L

N

N

P

G

G

G

binding acetyl coenzyme *a: S87 (= S87), I88 (≠ V88), Y89 (= Y89), I90 (≠ V90), K95 (≠ R95), G96 (= G96), G98 (= G98), G100 (= G100), K101 (≠ P101), G131 (≠ A129), S132 (= S130), K134 (≠ R132), L135 (= L133), H136 (= H134)

5wphA Crystal structure of arsn, n-acetyltransferase with substrate ast from pseudomonas putida kt2440 (see paper)
37% identity, 88% coverage: 5:160/177 of query aligns to 4:157/179 of 5wphA

query
sites
5wphA

I

I

R

R

D

V

A

A

V

R

Y

P

A

E

D

D

L

A

P

E

A

E

I

I

R

Q

D

I

I

I

Y

Y

N

A

D

P

A

I

V

V

L

L

N

N

T

T

T

-

A

A

I

I

W
x
S

N
x
F

E

E

Q

E

A

A

V

V

D

P

L

S

A

V

N

E

R

Q

Q

M

A

R

W

E

F

R

S

I

T

S

R

T

Q

T

S

L

Q

Q

G

T

Y

Y

P

P

V

Y

L

L

V

V

I

A

V

V

D

R

A

-

D

E

N

G

S

R

V

V

L

V

G

G

Y

Y

A

A

S

Y

F

A

G

S

D

Q

W

H

R
|
R

P

A

F
x
R

D

A

G

A

F
x
Y

R

R

H

W

T

A

V

V

E

D

H

V

S

T

V

V

Y

Y

V

V

R

A

S

E

D

G

Q

Q

R

R

G

R

N

S

G

G

L

I

G

A

P

R

Q

Q

L

L

M

Y

D

D

V

V

L

L

I

L

E

P

R

V

A

L

R

K

D

R

C

L

N

G

K

Y

H

R

V

S

M

A

V

Y

A

A

A

G

I

I

E

A

S

L

A

P

N

N

A

E

A

G

S

S

I

V

R

G

L

L

H

H

E

E

R

R

V

L

G

G

F

F

I

Q

T

H

T

I

G

G

H

T

M

F

P

P

Q

Q

V

V

G

G

T

F

K

K

F

L

G

D

R

A

W

W

L

H

D

D

L

V

T

G

F

Y

binding (2S)-2-amino-4-[hydroxy(methyl)arsoryl]butanoic acid: S29 (≠ W31), F30 (≠ N32), R72 (= R75), R74 (≠ F77), Y77 (≠ F80)

6m7gA Crystal structure of arsn, n-acetyltransferase with substrate phosphinothricin from pseudomonas putida kt2440 (see paper)
37% identity, 88% coverage: 5:160/177 of query aligns to 4:157/176 of 6m7gA

query
sites
6m7gA

I

I

R

R

D

V

A

A

V

R

Y

P

A

E

D

D

L

A

P

E

A

E

I

I

R

Q

D

I

I

I

Y

Y

N

A

D

P

A

I

V

V

L

L

N

N

T

T

T

-

A

A

I

I

W

S

N

F

E

E

Q

E

A

A

V

V

D

P

L

S

A

V

N

E

R

Q

Q

M

A

R

W

E

F

R

S

I

T

S

R

T

Q

T

S

L

Q

Q

G

T

Y

Y

P

P

V

Y

L

L

V

V

I

A

V

V

D

R

A

-

D

E

N

G

S

R

V

V

L

V

G

G

Y

Y

A

A

S

Y

F

A

G

S

D

Q

W

H

R

R

P

A

F
x
R

D

A

G

A

F
x
Y

R

R

H

W

T

A

V

V

E

D

H

V

S

T

V

V

Y

Y

V

V

R

A

S

E

D

G

Q

Q

R

R

G

R

N

S

G

G

L

I

G

A

P

R

Q

Q

L

L

M

Y

D

D

V

V

L

L

I

L

E

P

R

V

A

L

R

K

D

R

C

L

N

G

K

Y

H

R

V

S

M

A

V

Y

A

A

A

G

I

I

E

A

S

L

A

P

N

N

A

E

A

G

S

S

I

V

R

G

L

L

H

H

E

E

R

R

V

L

G

G

F

F

I

Q

T

H

T

I

G

G

H

T

M

F

P

P

Q

Q

V

V

G

G

T

F

K

K

F

L

G

D

R

A

W

W

L

H

D

D

L

V

T

G

F

Y

binding phosphinothricin: R74 (≠ F77), Y77 (≠ F80)

5t7eD Crystal structure of streptomyces hygroscopicus bialaphos resistance (bar) protein in complex with coenzyme a and l-phosphinothricin (see paper)
32% identity, 90% coverage: 5:163/177 of query aligns to 4:160/175 of 5t7eD

query
sites
5t7eD

I

I

R

R

D

R

A

A

V

T

Y

E

A

A

D

D

L

M

P

P

A

A

I

V

R

C

D

T

I

I

Y

V

N

N

D

H

A
x
Y

V

I

L

E

N

T

T

S

T

T

A
x
V

I

N

W
x
F

N

R

E

T

Q

E

A

P

V

Q

D

E

L

-

A

-

N

-

R

P

Q

Q

A

E

W

W

F

T

S

D

-

D

-

L

T

V

R

R

Q

L

S

R

Q

E

G

R

Y

Y

P

P

V

W

L

L

V

V

I

-

V

A

D

E

A

V

D

D

N

G

S

E

V

V

L

A

G

G

Y

I

A

A

S

Y

F

A

G

G

D

P

W

W

R
x
K

P

A

F
x
R

D

N

G

A

F
x
Y

R

D

H

W

T

T

V

A

E

E

H

S

S
x
T

V
|
V

Y
|
Y

V
|
V

R

S

S

P

D

R

Q

H

R
x
Q

G
x
R

N
x
T

G
|
G

L

L

G
|
G

P
x
S

Q

T

L

L

M

Y

D

T

V

H

L

L

I

L

E

K

R

S

A

L

R

E

D

A

C

Q

N

G

K

F

H

K

V

S

M

V

V

V

A

A

A
x
V

I

I

E
x
G

S

L

A

P

N

N

A

D

A
x
P

S
|
S

I

V

R

R

L
x
M

H

H

E

E

R

A

V

L

G

G

F

Y

I

A

T

P

T

R

G

G

H

M

M

L

P

R

Q

A

V

A

G

G

T

F

K

K

F

H

G

G

R

N

W

W

L

H

D

D

L

V

T

G

F

F

M

W

Q

Q

L

L

binding coenzyme a: Y22 (≠ A23), V28 (≠ A29), V85 (= V88), Y86 (= Y89), V87 (= V90), Q92 (≠ R95), R93 (≠ G96), T94 (≠ N97), G95 (= G98), G97 (= G100), S98 (≠ P101), P126 (≠ A129), S127 (= S130), M130 (≠ L133)
binding phosphinothricin: F30 (≠ W31), K72 (≠ R75), R74 (≠ F77), Y77 (≠ F80), T84 (≠ S87), Y86 (= Y89), V119 (≠ A122), G121 (≠ E124)

5t7dA Crystal structure of streptomyces hygroscopicus bialaphos resistance (bar) protein in complex with acetyl coenzyme a (see paper)
32% identity, 90% coverage: 5:163/177 of query aligns to 3:159/173 of 5t7dA

query
sites
5t7dA

I

I

R

R

D

R

A

A

V

T

Y

E

A

A

D

D

L

M

P

P

A

A

I

V

R

C

D

T

I

I

Y

V

N

N

D

H

A
x
Y

V

I

L

E

N

T

T

S

T

T

A
x
V

I

N

W

F

N

R

E

T

Q

E

A

P

V

Q

D

E

L

-

A

-

N

-

R

P

Q

Q

A

E

W

W

F

T

S

D

-

D

-

L

T

V

R

R

Q

L

S

R

Q

E

G

R

Y

Y

P

P

V

W

L

L

V

V

I

-

V

A

D

E

A

V

D

D

N

G

S

E

V

V

L

A

G

G

Y

I

A

A

S

Y

F

A

G

G

D

P

W

W

R

K

P

A

F

R

D

N

G

A

F

Y

R

D

H

W

T

T

V

A

E

E

H

S

S
x
T

V
|
V

Y
|
Y

V
|
V

R

S

S

P

D

R

Q

H

R
x
Q

G
x
R

N

T

G
|
G

L

L

G
|
G

P
x
S

Q

T

L

L

M

Y

D

T

V

H

L

L

I

L

E

K

R

S

A

L

R

E

D

A

C

Q

N

G

K

F

H

K

V

S

M

V

V

V

A

A

A
x
V

I

I

E

G

S

L

A

P

N

N

A

D

A
x
P

S
|
S

I

V

R
|
R

L
x
M

H

H

E

E

R

A

V

L

G

G

F

Y

I

A

T

P

T

R

G

G

H

M

M

L

P

R

Q

A

V

A

G

G

T

F

K

K

F

H

G

G

R

N

W

W

L

H

D

D

L

V

T

G

F

F

M

W

Q

Q

L

L

binding acetyl coenzyme *a: Y21 (≠ A23), V27 (≠ A29), T83 (≠ S87), V84 (= V88), Y85 (= Y89), V86 (= V90), Q91 (≠ R95), R92 (≠ G96), G94 (= G98), G96 (= G100), S97 (≠ P101), V118 (≠ A122), P125 (≠ A129), S126 (= S130), R128 (= R132), M129 (≠ L133)

Query Sequence

>AO353_13510 FitnessBrowser__pseudo3_N2E3:AO353_13510
MTYHIRDAVYADLPAIRDIYNDAVLNTTAIWNEQAVDLANRQAWFSTRQSQGYPVLVIVD
ADNSVLGYASFGDWRPFDGFRHTVEHSVYVRSDQRGNGLGPQLMDVLIERARDCNKHVMV
AAIESANAASIRLHERVGFITTGHMPQVGTKFGRWLDLTFMQLILNPGEEPPVANKE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory