SitesBLAST

Q8U122 Uridylate kinase; UK; Uridine monophosphate kinase; UMP kinase; UMPK; EC 2.7.4.22 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see paper)
38% identity, 29% coverage: 153:261/372 of query aligns to 120:225/225 of Q8U122

query
sites
Q8U122

N
x
T

D
|
D

T

A

L

V

A

A

L

L

V

L

A

A

N

E

L

F

V

L

E

K

A

A

D

D

L

L

V

V

I

V

L

I

T

T

D

N

R

V

D

D

G

G

M

V

F

Y

D

T

A

A

D

D

P

P

R

K

N

K

N

D

P

P

D

T

A

A

K

K

L

K

I

I

Y

K

E

K

A

M

R

K

A

P

D

E

P

-

A

L

L

L

D

E

A

I

V

V

A

G

G

K

G

G

T

I

G

E

G

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A

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G

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G
x
S

M

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I

T

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P

L

L

R

-

A

A

R

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L

I

A

I

A

A

R

R

S

S

G

G

A

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K

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T

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V

I

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G

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D

F

R

R

L

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K

I

A

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E

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N

G

G

T

T

L

T

L

I

S

E

P

P

T120 (≠ N153) binding
D121 (= D154) binding ; binding
G179 (= G214) binding
S182 (≠ G217) binding

Sites not aligning to the query:

6 binding
44 binding
66 binding
114:120 binding

2bmuB Ump kinase from pyrococcus furiosus complexed with its substrate ump and its substrate analog amppnp (see paper)
38% identity, 29% coverage: 153:261/372 of query aligns to 121:226/226 of 2bmuB

query
sites
2bmuB

N
x
T

D
|
D

T

A

L

V

A

A

L

L

V

L

A

A

N

E

L

F

V

L

E

K

A

A

D

D

L

L

V

V

I

V

L

I

T
|
T

D
x
N

R

V

D

D

G

G

M
x
V

F
x
Y

D

T

A
|
A

D
|
D

P
|
P

R

K

N

K

N

D

P

P

D

T

A

A

K

K

L

K

I

I

Y

K

E

K

A

M

R

K

A

P

D

E

P

-

A

L

L

L

D

E

A

I

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V

A

G

G

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G

G

T

I

G

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G

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A
|
A

L

-

G

G

R

S

G
x
S

M
x
V

Q

I

T

D

K

P

L

L

R

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A

A

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A

I

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A

R

R

S

S

G

G

A

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T

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V

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E

L

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E

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L

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A

K

G

G

E

D

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N

G

G

T

T

L

T

L

I

S

E

P

P

binding phosphoaminophosphonic acid-adenylate ester: T141 (= T173), N142 (≠ D174), V146 (≠ M178), Y147 (≠ F179), A149 (= A181), D150 (= D182), P151 (= P183), S182 (≠ G216), S183 (≠ G217), V184 (≠ M218)
binding magnesium ion: D122 (= D154), D122 (= D154), S183 (≠ G217), V184 (≠ M218)
binding uridine-5'-monophosphate: T121 (≠ N153), A179 (= A212)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 9, 10, 44, 45, 46
binding magnesium ion: 7
binding uridine-5'-monophosphate: 44, 45, 67, 70, 71, 114, 115, 116, 119, 120

2ji5A Structure of ump kinase from pyrococcus furiosus complexed with utp
34% identity, 29% coverage: 153:261/372 of query aligns to 122:219/219 of 2ji5A

query
sites
2ji5A

N
x
T

D

D

T

A

L

V

A

A

L

L

V

L

A

A

N

E

L

F

V

L

E

K

A

A

D

D

L

L

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I

V

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T

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N

R

V

D

D

G

G

M

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Y

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A

A

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N

K

N

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P

D

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A

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K

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M

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A

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V

A

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G

K

G

S

T

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G

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A

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L

L

G

-

R

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G

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M

-

Q

-

T

-

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-

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A

A

R

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I

A

I

A

A

R

R

S

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G

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A

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G

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T

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L

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L

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P

binding uridine 5'-triphosphate: T122 (≠ N153)

Sites not aligning to the query:

binding uridine 5'-triphosphate: 68, 71, 72, 115, 116, 117, 120, 121

8u0mA Isopentenyl phosphate kinase (see paper)
24% identity, 53% coverage: 62:259/372 of query aligns to 67:243/247 of 8u0mA

query
sites
8u0mA

S

K

R

R

L

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G

W

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T

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A

K

R

T

P

H

S

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A

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M

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K

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L

Q

N

A

D

A

L

A

-

I

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G

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M

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A

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W

F

-

L

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A

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Y

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H

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D

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L

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V

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Y

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L

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N

F

E
x
G

N

D

D

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T

A

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V

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D

D

E

K

-

G

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F
x
I

G

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N
x
G

D

D

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Q

L

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A

V

A

S

L

Y

V

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A

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N

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V

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E

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A

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D

S

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M

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D

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x
V

D

D

G

G

M

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x
Y

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R

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N

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|
P

R

K

N

E

N

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D

D

A

A

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K

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E

E

E

A

L

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N

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D

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A

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V

-

A

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T

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S

G
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G

M
x
I

Q

G

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K

L

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A

E

A

A

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N

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L

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G

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E

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K

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G

T

T

L

R

L

L

binding adenosine-5'-diphosphate: V171 (≠ R175), Y175 (≠ F179), R177 (≠ A181), P179 (= P183), G202 (= G217), I203 (≠ M218), K206 (= K221)
binding (2E)-2-methylbut-2-en-1-yl dihydrogen phosphate: G134 (≠ E139), I146 (≠ F150), G149 (≠ N153)

Sites not aligning to the query:

8u0mB Isopentenyl phosphate kinase (see paper)
23% identity, 53% coverage: 62:259/372 of query aligns to 68:243/247 of 8u0mB

query
sites
8u0mB

S

K

R

R

L

I

G

G

W

F

T

S

A

K

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T

P

H

S

Q

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A

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M

H

L

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K

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L

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N

A

D

A

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A

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M

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A

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Y

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S

Q

S

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F

L

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H

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D

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L

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Y

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Y

N

G

A

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R

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D

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P

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V

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L

N

F

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x
G

N

D

D

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T

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T

L

D

D

E

K

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G

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G

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D

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M

D

D

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D

D

G

G

M

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F
x
Y

D

D

A
x
R

D

N

P
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P

R

K

N

E

N

R

P

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D

D

A

A

K

K

L

L

I

I

Y

E

E

E

A

L

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N

A

V

D

E

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I

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R

P

H

A

L

L

L

D

E

A

S

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x
I

A

G

G

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G

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A

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L

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G

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M

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Q

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N

K
|
K

L

L

R

R

A

E

A

A

R

L

L

K

A

I

A

A

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K

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G

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A

S

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E

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G

T

T

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L

binding adenosine-5'-triphosphate: Y176 (≠ F179), R178 (≠ A181), P180 (= P183), I203 (≠ V205), K206 (= K221)
binding (2E)-2-methylbut-2-en-1-yl dihydrogen phosphate: G135 (≠ E139)

Sites not aligning to the query:

8u0lA Isopentenyl phosphate kinase (see paper)
24% identity, 53% coverage: 62:259/372 of query aligns to 68:243/247 of 8u0lA

query
sites
8u0lA

S

K

R

R

L

I

G

G

W

F

T

S

A

K

R

T

P

H

S

Q

A

A

M

M

H

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K

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N

A

D

A

L

A

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I

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G

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Q

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M

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G

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Q

A

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F

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L

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E

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K

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T

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A

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S

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L

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H

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D

-

L

L

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N

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Y

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A

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A

K

L

L

V

L

D

E

L

L

K

K

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F

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P

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V

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N

F

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x
G

N

D

D

T

T

A

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I

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A

T

L

D

D

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K

-

G

I

I

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D

F
x
I

G

L

D

S

N

G

D

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S

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Y

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A

A

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K

L

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K

A

P

D

S

L

K

L

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V

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I

F

L

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M

D

D

R
x
V

D

D

G

G

M

I

F
x
Y

D

D

A
x
R

D

N

P
|
P

R

K

N

E

N

R

P

-

D

D

A

A

K

K

L

L

I

I

Y

E

E

E

A

L

R

N

A

V

D

E

-

I

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R

P

H

A

L

L

L

D

E

A

S

V
x
I

A

G

G

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T

-

G

-

A

-

L

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R

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G

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M

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K

L

L

R

R

A

E

A

A

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L

R

K

L

I

A

A

A

K

R

H

S

S

G

E

A

V

H

Y

T

F

I

I

I

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V

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G

N

G

G

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K

L

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K

R

E

V

N

L

L

D

G

R

K

L

A

K

I

A

R

G

G

E

E

R

K

I

V

G

G

T

T

L

R

L

L

binding adenosine-5'-diphosphate: V172 (≠ R175), Y176 (≠ F179), R178 (≠ A181), P180 (= P183), I203 (≠ V205), K206 (= K221)
binding (2E)-but-2-en-1-yl dihydrogen phosphate: G135 (≠ E139), I147 (≠ F150)

Sites not aligning to the query:

8u0kA Isopentenyl phosphate kinase (see paper)
24% identity, 53% coverage: 62:259/372 of query aligns to 68:243/247 of 8u0kA

query
sites
8u0kA

S

K

R

R

L

I

G

G

W

F

T

S

A

K

R

T

P

H

S

Q

A

A

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M

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K

L

L

Q

N

A

D

A

L

A

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I

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M

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G

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L

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A

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F

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E

A

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Y

G

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A

S

Q

S

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I

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F

L

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H

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D

-

L

L

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E

D

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K

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Y

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L

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R

A

K

L

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V

L

D

E

L

L

K

K

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P

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V

I

L

N

F

E
x
G

N

D

D

T

T

A

V

I

V

A

T

L

D

D

E

K

-

G

I

I

R

D

F
x
I

G

L

D
x
S

N
x
G

D

D

T

Q

L

I

A

V

A

S

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Y

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A

A

N

K

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D

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V

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I

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G

M
x
I

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x
Y

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D

A
x
R

D

N

P
|
P

R

K

N

E

N

R

P

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D

D

A

A

K

K

L

L

I

I

Y

E

E

E

A

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A

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D

E

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I

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H

A

L

L

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E

A

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x
I

A

G

G

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G

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A

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G

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M

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K

L

L

R

R

A

E

A

A

-

L

R

K

L

I

A

A

A

K

R

H

S

S

G

E

A

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H

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T

F

I

I

I

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G

N

G

G

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K

R

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N

L

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D

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K

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A

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I

A

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G

G

E

E

R

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I

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G

G

T

T

L

R

L

L

binding Dimethylallyl monophosphate: G135 (≠ E139), I147 (≠ F150), S149 (≠ D152), G150 (≠ N153)
binding adenosine-5'-diphosphate: I175 (≠ M178), Y176 (≠ F179), R178 (≠ A181), P180 (= P183), I203 (≠ V205), K206 (= K221)

Sites not aligning to the query:

binding Dimethylallyl monophosphate: 44, 49

8u0nA Isopentenyl phosphate kinase (see paper)
23% identity, 53% coverage: 62:259/372 of query aligns to 66:241/245 of 8u0nA

query
sites
8u0nA

S

K

R

R

L

I

G

G

W

F

T

S

A

K

R

T

P

H

S

Q

A

A

M
|
M

H

L

E

K

L

L

Q

N

A

D

A

L

A

-

I

-

G

-

Q

-

M

-

G

-

L

I

V

I

Q

Q

A

T

W

F

-

L

E

E

S

K

S

G

F

L

A

P

E

A

H

Y

G

S

R

V

H

S

T

S

A

S

Q

S

I

I

L

F

L

L

T

-

H

-

D

-

L

L

S

E

D

N

R

K

K

E

R

V

Y

V

L

Y

N

G

A

E

R

L

S

E

T

I

L

L

R

R

A

K

L

L

V

L

D

E

L

L

K

K

V

F

I

I

P

P

V

V

I

L

N

F

E
x
G

N

D

D

T

T

A

V

I

V

A

T

L

D

D

E

K

-

G

I

I

R

D

F
x
I

G

L

D
x
S

N
x
G

D

D

T

Q

L

I

A

V

A

S

L

Y

V

L

A

A

N

K

L

M

V

L

E

K

A

P

D

S

L

K

L

V

V

I

I

F

L

L

T

M

D

D

R

V

D

D

G

G

M
x
I

F
x
Y

D

D

A
x
R

D

N

P
|
P

R

K

N

E

N

R

P

-

D

D

A

A

K

K

L

L

I

I

Y

E

E

E

A

L

R

N

A

V

D

E

-

I

-

R

P

H

A

L

L

L

D

E

A

S

V
x
I

A

G

G

-

T

-

G

-

A

-

L

-

G

-

R

-

G

-

M

-

Q

-

T

N

K
|
K

L

L

R

R

A

E

A

A

R

L

L

K

A

I

A

A

R

K

S

H

G

-

A

S

H

E

T

V

I

Y

I

F

V

I

G

N

G

G

R

K

L

V

E

K

R

E

V

N

L

L

D

G

R

K

L

A

K

I

A

R

G

G

E

E

R

K

I

V

G

G

T

T

L

R

L

L

binding adenosine-5'-diphosphate: I173 (≠ M178), Y174 (≠ F179), R176 (≠ A181), P178 (= P183), I201 (≠ V205), K204 (= K221)
binding 2-cyclopentylideneethyl dihydrogen phosphate: M77 (= M73), G133 (≠ E139), I145 (≠ F150), S147 (≠ D152), G148 (≠ N153)

Sites not aligning to the query:

3ll5A Crystal structure of t. Acidophilum isopentenyl phosphate kinase product complex (see paper)
36% identity, 19% coverage: 123:192/372 of query aligns to 113:183/233 of 3ll5A

query
sites
3ll5A

S

T

T

P

L

L

R

I

A

R

L

Y

V

I

D

D

L

A

K

G

V

F

I

V

P

P

V

V

I

-

N

S

E

Y

N
x
G

D

D

T

V

V

Y

V

I

T

K

D

D

E

E

I

H

R

S

F

Y

G

G

-

I

-

Y

D
x
S

N
x
G

D

D

T

D

L

I

A

M

A

A

L

D

V

M

A

A

N

E

L

L

V

L

E

K

A

P

D

D

L

V

L

A

V

V

I

F

L

L

T

T

D
|
D

R

V

D

D

G

G

M

I

F
x
Y

D

S

A

K

D
|
D

P
|
P

R
x
K

N

R

N

N

P

P

D

D

A

A

K

V

L

L

I

L

binding adenosine-5'-diphosphate: D165 (= D174), Y170 (≠ F179), D173 (= D182), P174 (= P183), K175 (≠ R184)
binding 3-methylbut-3-enyl trihydrogen diphosphate: G129 (≠ N140), S143 (≠ D152), G144 (≠ N153)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 8, 10, 15, 190, 193
binding 3-methylbut-3-enyl trihydrogen diphosphate: 6, 9, 15, 45, 46, 47, 50, 51

3lkkB Crystal structure of the isopentenyl phosphate kinase substrate complex (see paper)
36% identity, 19% coverage: 123:192/372 of query aligns to 114:184/238 of 3lkkB

query
sites
3lkkB

S

T

T

P

L

L

R

I

A

R

L

Y

V

I

D

D

L

A

K

G

V

F

I

V

P

P

V

V

I

-

N

S

E

Y

N
x
G

D
|
D

T

V

V

Y

V

I

T

K

D

D

E

E

I

H

R

S

F

Y

G

G

-
x
I

-

Y

D
x
S

N
x
G

D

D

T

D

L

I

A

M

A

A

L

D

V

M

A

A

N

E

L

L

V

L

E

K

A

P

D

D

L

V

L

A

V

V

I

F

L

L

T

T

D
|
D

R
x
V

D

D

G

G

M

I

F
x
Y

D

S

A

K

D
|
D

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|
P

R
x
K

N

R

N

N

P

P

D

D

A

A

K

V

L

L

I

L

binding adenosine-5'-triphosphate: D166 (= D174), V167 (≠ R175), Y171 (≠ F179), D174 (= D182), P175 (= P183), K176 (≠ R184)
binding Isopentenyl phosphate: G130 (≠ N140), D131 (= D141), I142 (vs. gap), S144 (≠ D152), G145 (≠ N153)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 7, 9, 10, 11, 16, 48, 52, 195, 198
binding Isopentenyl phosphate: 7, 46, 47, 51, 52

Query Sequence

>AO353_14115 FitnessBrowser__pseudo3_N2E3:AO353_14115
MRSKVTGAQRWVVKIGSALLTADGKGLDRAAMSVWVEQMVALHEAGVELVLVSSGAVAAG
MSRLGWTARPSAMHELQAAAAIGQMGLVQAWESSFAEHGRHTAQILLTHDDLSDRKRYLN
ARSTLRALVDLKVIPVINENDTVVTDEIRFGDNDTLAALVANLVEADLLVILTDRDGMFD
ADPRNNPDAKLIYEARADDPALDAVAGGTGGALGRGGMQTKLRAARLAARSGAHTIIVGG
RLERVLDRLKAGERIGTLLSPERGLLAARKQWLAGHLQTRGTLVLDEGAVTALSRGNKSL
LPVGVKLVQGSFRRGEMVVCVAPDGREIARGLANYSALEAQKIIGQSSEAIVGLLGYMAE
PELVHRDNLILV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory