SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO353_15550 FitnessBrowser__pseudo3_N2E3:AO353_15550 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

Q88E47 Homogentisate 1,2-dioxygenase; HGDO; Homogentisate oxygenase; Homogentisic acid oxidase; Homogentisicase; EC 1.13.11.5 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
83% identity, 100% coverage: 1:434/434 of query aligns to 1:433/433 of Q88E47

query
sites
Q88E47

M

M

N

N

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R

D

D

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-

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E

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A

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H288 (= H289) active site, Proton acceptor; mutation to F: Reduces the catalytic efficiency 75-fold.
Y346 (= Y347) binding homogentisate; mutation to F: Decreases the affinity for homogentisate more than 60-fold and reduces the catalytic efficiency 20-fold.
H367 (= H368) binding homogentisate

4aq6D Substrate bound homogentisate 1,2-dioxygenase (see paper)
84% identity, 98% coverage: 8:434/434 of query aligns to 1:427/427 of 4aq6D

query
sites
4aq6D

P

P

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active site: H282 (= H289), H325 (= H332), E331 (= E338), H355 (= H362), H361 (= H368)
binding fe (iii) ion: H325 (= H332), E331 (= E338), H361 (= H368)
binding 2-(3,6-dihydroxyphenyl)acetic acid: H282 (= H289), P285 (= P292), P322 (= P329), W323 (= W330), E331 (= E338), M333 (= M340), Y340 (= Y347), H361 (= H368)

3zdsA Structure of homogentisate 1,2-dioxygenase in complex with reaction intermediates of homogentisate with oxygen. (see paper)
84% identity, 98% coverage: 10:434/434 of query aligns to 1:425/425 of 3zdsA

query
sites
3zdsA

L

L

V

H

Y

Y

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L

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F

G

G

N

N

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E

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A

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L

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L

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L

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D

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L

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I

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G

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N

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L

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A

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D

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D

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F

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F

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P

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W

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H

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M

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E

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M

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E

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V

L

L

R

R

P

P

S

S

R

L

F

Q

A

A

L

L

D

E

C

C

P

P

Q

Q

L

L

Q

Q

N

A

N

D

Y

Y

D

D

A

S

C

C

W

W

A

A

S

T

L

L

P

P

V

S

T

T

F

F

N

N

P

P

N

N

R

R

R

R

active site: H280 (= H289), H323 (= H332), E329 (= E338), H353 (= H362), H359 (= H368)
binding fe (iii) ion: H323 (= H332), E329 (= E338), H359 (= H368)
binding 4-Maleylacetoacetic acid: H280 (= H289), P283 (= P292), P320 (= P329), W321 (= W330), H323 (= H332), M331 (= M340), Y338 (= Y347), H359 (= H368), M386 (= M395)

3zdsF Structure of homogentisate 1,2-dioxygenase in complex with reaction intermediates of homogentisate with oxygen. (see paper)
84% identity, 98% coverage: 10:434/434 of query aligns to 2:426/426 of 3zdsF

query
sites
3zdsF

L

L

V

H

Y

Y

Q

L

S

S

G

G

F

F

G

G

N

N

E

E

F

F

S

A

S

S

E

E

A

A

L

L

P

P

G

G

A

A

L

L

P

P

V

V

G

G

Q

Q

N

N

S

S

P

P

Q

Q

K

K

A

A

P

P

Y

Y

G

G

L

L

Y

Y

T

A

E

E

L

L

F

L

S

S

G

G

T

T

A

A

F

F

T

T

M

M

A

A

R

R

S

S

E

E

A

L

R

R

R

R

T

T

W

W

M

L

Y

Y

R

R

I

I

Q

R

P

P

S

S

A

A

N

L

H

H

P

P

A

R

F

F

V

E

K

R

L

L

E

A

R

R

Q

Q

L

P

A

L

G

G

G

G

P

P

L

L

G

G

D

G

V

I

T

N

P

P

N

N

R

R

L

L

R

R

W

W

N

S

P

P

L

Q

E

P

I

I

P

P

G

A

E

E

P

P

T

T

D

D

F

F

I

I

D

E

G

G

L

W

V

L

R

P

M

M

A

A

A

A

N

N

S

A

G

G

S

A

E

E

K

K

P

P

A

A

G

G

I

V

S

S

I

I

Y

Y

H

I

Y

Y

R

R

A

A

N

N

R

R

S

S

M

M

E

E

R

R

V

V

F

F

F

F

N

N

A

A

D

D

G

G

E

E

W

L

L

L

L

L

V

V

P

P

E

E

Q

Q

G

G

R

R

L

L

R

R

I

I

A

A

T

T

E

E

L

L

G

G

V

V

L

M

E

E

L

V

E

E

P

P

L

L

E

E

I

I

A

A

V

V

L

I

P

P

R

R

G

G

L

M

K

K

F

F

R

R

I

V

E

E

L

L

L

L

D

D

P

G

Q

Q

A

A

R

R

G

G

Y

Y

L

I

A

A

E

E

N

N

H

H

G

G

A

A

P

P

L

L

R

R

L

L

P

P

D

D

L

L

G

G

P

P

I

I

G

G

S

S

N

N

G

G

L

L

A

A

N

N

P

P

R

R

D

D

F

F

L

L

T

T

P

P

V

V

A

A

H

H

Y

Y

E

E

D

E

L

A

K

E

Q

G

P

P

T

V

T

Q

L

L

V

V

Q

Q

K

K

F

F

L

L

G

G

E

E

L

H

W

W

G

A

C

C

E

E

L

L

D

Q

H

H

S

S

P

P

L

L

N

D

V

V

V

V

A

A

W

W

H

H

G

G

N

S

N

N

V

V

P

P

Y

Y

K

K

Y

Y

D

D

L

L

R

R

R

R

F

F

N

N

T

T

I

I

G

G

T

T

V

V

S

S

F

F

D

D

H
|
H

P

P

D

D

P
|
P

S

S

I

I

F

F

T

T

V

V

L

L

T

T

S

S

P

P

T

T

S

S

V

V

H

H

G

G

L

M

A

A

N

N

L

M

D

D

F

F

V

V

I

I

F

F

P

P

P

P

R

R

W

W

M

M

V

V

A

A

E

E

N

N

T

T

F

F

R

R

P

P

P
|
P

W
|
W

F

F

H
|
H

R

R

N

N

L

L

M

M

N

N

E
|
E

F

F

M
|
M

G

G

L

L

I

I

Q

N

G

G

T

A

Y
|
Y

D

D

A

A

K

K

A

A

E

E

G

G

F

F

L

L

P

P

G

G

G

G

A

A

S

S

L

L

H
|
H

S

G

C

V

M

M

S

S

A

A

H
|
H

G

G

P

P

D

D

G

A

E

E

T

T

C

C

T

E

K

K

A

A

I

I

N

A

A

A

E

D

L

L

A

A

P

P

S

H

K

K

I

I

D

D

N

N

T

T

M

M

A

A

F

F

M
|
M

F

F

E

E

T

T

S

S

Q

Q

V

V

L

L

R

R

P

P

S

S

R

L

F

Q

A

A

L

L

D

E

C

C

P

P

Q

Q

L

L

Q

Q

N

A

N

D

Y

Y

D

D

A

S

C

C

W

W

A

A

S

T

L

L

P

P

V

S

T

T

F

F

N

N

P

P

N

N

R

R

R

R

active site: H281 (= H289), H324 (= H332), E330 (= E338), H354 (= H362), H360 (= H368)
binding fe (iii) ion: H324 (= H332), E330 (= E338), H360 (= H368)
binding 2-[(6R)-6-(dioxidanyl)-6-oxidanyl-3-oxidanylidene-cyclohexa-1,4-dien-1-yl]ethanoic acid: H281 (= H289), P284 (= P292), P321 (= P329), W322 (= W330), H324 (= H332), E330 (= E338), M332 (= M340), Y339 (= Y347), H360 (= H368), M387 (= M395)

3zdsC Structure of homogentisate 1,2-dioxygenase in complex with reaction intermediates of homogentisate with oxygen. (see paper)
84% identity, 98% coverage: 10:434/434 of query aligns to 2:426/426 of 3zdsC

query
sites
3zdsC

L

L

V

H

Y

Y

Q

L

S

S

G

G

F

F

G

G

N

N

E

E

F

F

S

A

S

S

E

E

A

A

L

L

P

P

G

G

A

A

L

L

P

P

V

V

G

G

Q

Q

N

N

S

S

P

P

Q

Q

K

K

A

A

P

P

Y

Y

G

G

L

L

Y

Y

T

A

E

E

L

L

F

L

S

S

G

G

T

T

A

A

F

F

T

T

M

M

A

A

R

R

S

S

E

E

A

L

R

R

R

R

T

T

W

W

M

L

Y

Y

R

R

I

I

Q

R

P

P

S

S

A

A

N

L

H

H

P

P

A

R

F

F

V

E

K

R

L

L

E

A

R

R

Q

Q

L

P

A

L

G

G

G

G

P

P

L

L

G

G

D

G

V

I

T

N

P

P

N

N

R

R

L

L

R

R

W

W

N

S

P

P

L

Q

E

P

I

I

P

P

G

A

E

E

P

P

T

T

D

D

F

F

I

I

D

E

G

G

L

W

V

L

R

P

M

M

A

A

A

A

N

N

S

A

G

G

S

A

E

E

K

K

P

P

A

A

G

G

I

V

S

S

I

I

Y

Y

H

I

Y

Y

R

R

A

A

N

N

R

R

S

S

M

M

E

E

R

R

V

V

F

F

F

F

N

N

A

A

D

D

G

G

E

E

W

L

L

L

L

L

V

V

P

P

E

E

Q

Q

G

G

R

R

L

L

R

R

I

I

A

A

T

T

E

E

L

L

G

G

V

V

L

M

E

E

L

V

E

E

P

P

L

L

E

E

I

I

A

A

V

V

L

I

P

P

R

R

G

G

L

M

K

K

F

F

R

R

I

V

E

E

L

L

L

L

D

D

P

G

Q

Q

A

A

R

R

G

G

Y

Y

L

I

A

A

E

E

N

N

H

H

G

G

A

A

P

P

L

L

R

R

L

L

P

P

D

D

L

L

G

G

P

P

I

I

G

G

S

S

N

N

G

G

L

L

A

A

N

N

P

P

R

R

D

D

F

F

L

L

T

T

P

P

V

V

A

A

H

H

Y

Y

E

E

D

E

L

A

K

E

Q

G

P

P

T

V

T

Q

L

L

V

V

Q

Q

K

K

F

F

L

L

G

G

E

E

L

H

W

W

G

A

C

C

E

E

L

L

D

Q

H

H

S

S

P

P

L

L

N

D

V

V

V

V

A

A

W

W

H

H

G

G

N

S

N

N

V

V

P

P

Y

Y

K

K

Y

Y

D

D

L

L

R

R

R

R

F

F

N

N

T

T

I

I

G

G

T

T

V

V

S

S

F

F

D

D

H
|
H

P

P

D

D

P
|
P

S

S

I

I

F

F

T

T

V

V

L

L

T

T

S

S

P

P

T

T

S

S

V

V

H

H

G

G

L

M

A

A

N

N

L

M

D

D

F

F

V

V

I

I

F
|
F

P

P

P

P

R

R

W

W

M

M

V

V

A

A

E

E

N

N

T

T

F

F

R

R

P

P

P
|
P

W

W

F

F

H
|
H

R

R

N

N

L

L

M

M

N

N

E
|
E

F

F

M
|
M

G

G

L

L

I

I

Q

N

G

G

T

A

Y
|
Y

D

D

A

A

K

K

A

A

E

E

G

G

F

F

L

L

P

P

G

G

G

G

A

A

S

S

L

L

H
|
H

S

G

C

V

M

M

S

S

A

A

H
|
H

G

G

P

P

D

D

G

A

E

E

T

T

C

C

T

E

K

K

A

A

I

I

N

A

A

A

E

D

L

L

A

A

P

P

S

H

K

K

I

I

D

D

N

N

T

T

M

M

A

A

F

F

M

M

F

F

E

E

T

T

S

S

Q

Q

V

V

L

L

R

R

P

P

S

S

R

L

F

Q

A

A

L

L

D

E

C

C

P

P

Q

Q

L

L

Q

Q

N

A

N

D

Y

Y

D

D

A

S

C

C

W

W

A

A

S

T

L

L

P

P

V

S

T

T

F

F

N

N

P

P

N

N

R

R

R

R

active site: H281 (= H289), H324 (= H332), E330 (= E338), H354 (= H362), H360 (= H368)
binding fe (iii) ion: H324 (= H332), E330 (= E338), H360 (= H368)
binding 2-(6-oxidanyl-3-oxidanylidene-cyclohexa-1,4-dien-1-yl)ethanoic acid: H281 (= H289), P284 (= P292), F307 (= F315), P321 (= P329), E330 (= E338), M332 (= M340), Y339 (= Y347), H360 (= H368)
binding oxygen molecule: P284 (= P292), H324 (= H332), E330 (= E338)

Q93099 Homogentisate 1,2-dioxygenase; Homogentisate oxygenase; Homogentisic acid oxidase; Homogentisicase; EC 1.13.11.5 from Homo sapiens (Human) (see 13 papers)
51% identity, 98% coverage: 10:434/434 of query aligns to 4:439/445 of Q93099

query
sites
Q93099

L

L

V

K

Y

Y

Q

I

S

S

G

G

F

F

G

G

N

N

E

E

F

C

S

S

S

S

E

E

-

D

-

P

A

R

L

C

P

P

G

G

A

S

L

L

P

P

V

E

G

G

Q

Q

N

N

S

N

P

P

Q

Q

K

V

A

C

P

P

Y

Y

G

N

L

L

Y

Y

T

A

E

E

L

Q

F

L

S

S

G

G

T

S

A

A

F

F

T

T

M

C

A

P

R

R

S

S

E

T

A

N

R

K

R

R

T

S

W

W

M

L

Y

Y

R

R

I

I

Q

L

P

P

S

S

A

V

N

S

H

H

P

K

A

P

F

F

V

E

K

S

L

I

E

D

R

E

Q

G

L
x
Q

A

V

G

T

G

H

P

N

L

W

G

D

D

E

V

V

T

D

P

P

-

D

-

P

N

N

R

Q

L

L

R

R

W

W

N

K

P

P

L

F

E

E

I

I

P

P

G

K

-

A

-

S

-

Q

E

K

P

K

T

V

D

D

F

F

I

V

D

S

G

G

L

L

V

H

R

T

M

L

A

C

A

G

N

A

S

G

G

D

S

I

E

K

K

S

P

N

A

N

G

G

I

L

S

A

I

I

Y

H

H

I

Y

F

R

L

A

C

N

N

R

T

S

S

M

M

E

E

-

N

R

R

V

C

F

F

F

Y

N

N

A

S

D

D

G

G

E

D

W

F

L

L

L

I

V

V

P

P

E

Q

Q

K

G
|
G

R

N

L

L

R

L

I

I

A

Y

T

T

E
|
E

L

F

G

G

V

K

L
x
M

E

L

L

V

E

Q

P

P

L

N

E

E

I

I

A

C

V

V

L

I

P

Q

R

R

G

G

L

M

K

R

F

F

R

S

I

I

E

D

L

V

L

F

D

E

P

-

Q

E

A

T

R

R

G

G

Y

Y

L

I

A

L

E

E

N

V

H

Y

G

G

A

V

P

H

L

F

R

E

L

L

P

P

D

D

L

L

G

G

P

P

I

I

G

G

S

A

N

N

G

G

L

L

A

A

N

N

P

P

R

R

D

D

F

F

L

L

T

I

P
|
P

V

I

A

A

H

W

Y

Y

E

E

D

D

L

R

K

Q

Q

V

P

P

-

G

-

G

T

Y

T

T

L

V

V

I

Q

N

K

K

F

Y

L

Q

G

G

E

K

L

L

W

F

G

A

C

A

E

K

L

Q

D

D

H

V

S

S

P

P

L

F

N

N

V

V

V

V

A

A

W

W

H

H

G

G

N

N

N

Y

V

T

P

P

Y

Y

K

K

Y

Y

D

N

L

L

R

K

R

N

F

F

N

M

T

V

I

I

G

N

T

S

V

V

S

A

F

F

D

D

H

H

P

A

D

D

P

P

S

S

I

I

F

F

T

T

V

V

L

L

T

T

S

A

P

K

T

S

S

V

V

R

H

P

G

G

L

V

A

A

N

I

L

A

D

D

F

F

V

V

I

I

F

F

P

P

P

P

R

R

W

W

M

G

V

V

A

A

E

D

N

K

T

T

F

F

R

R

P

P

P

P

W

Y

F

Y

H
|
H

R

R

N

N

L

C

M

M

N

S

E
|
E

F

F

M

M

G

G

L

L

I

I

Q

R

G

G

T

H

Y

Y

D

E

A

A

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K

A

Q

E

G

G

G

F

F

L

L

P

P

G

G

G

G

A

G

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S

L

L

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H

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M
|
M

S

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A

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H
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H

G

G

P

P

D

D

G

A

E

D

T

C

C

F

T

E

K

K

A

A

I

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N

K

A

V

E

K

L

L

A

A

P

P

S

E

K

R

I

I

-

A

D

D

N

G

T

T

M

M

A

A

F

F

M

M

F

F

E

E

T

S

S

S

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L

V

S

L

L

R

A

P

V

S

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R

K

F

W

A

G

L

L

D

K

C

A

P

S

Q

R

-

C

L

L

Q

D

N

E

N

N

Y

Y

D

H

A

K

C

C

W

W

A

E

S

P

L

L

P

K

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T

H

F

F

N

T

P

P

N

N

R

S

R

R

Q80 (≠ L84) to H: in dbSNP:rs2255543
G161 (= G159) to R: in AKU; loss of activity; most prevalent mutation in Slovak and Czech patients; dbSNP:rs28941783
E168 (= E166) to K: in AKU; loss of activity; dbSNP:rs375283568
M172 (≠ L170) natural variant: M -> T
P230 (= P229) to S: in AKU; complete loss of activity; dbSNP:rs28942100
H335 (= H332) binding Fe cation
E341 (= E338) binding Fe cation
M368 (= M365) to V: in AKU; loss of activity; dbSNP:rs120074173
H371 (= H368) binding Fe cation

Q9Y041 Homogentisate 1,2-dioxygenase; Homogentisate oxygenase; Homogentisic acid oxidase; Homogentisicase; EC 1.13.11.5 from Caenorhabditis elegans (see paper)
49% identity, 97% coverage: 10:429/434 of query aligns to 7:433/437 of Q9Y041

query
sites
Q9Y041

L

L

V

K

Y

Y

Q

L

S

T

G

G

F

F

G

G

N

N

E

E

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H

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A

S

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E

S

-

D

-

P

A

R

L

V

P

P

G

D

A

A

L

L

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P

V

V

G

G

Q

Q

N

N

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S

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P

Q

Q

K

K

A

C

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S

Y

H

G

G

L

L

Y

Y

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A

E

E

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T

A

A

F

F

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A

A

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A

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Q

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W

M

L

Y

Y

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I

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H

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A

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F

-

E

-

A

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M

K

K

L

E

E

N

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Q

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H

A

W

G

T

G

N

P

N

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G

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D

S

V

I

-

P

-

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T

N

P

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L

Y

R

R

W

W

N

N

P

P

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F

E

P

I

L

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-

T

G

K

E

E

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G

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T

F

F

I

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D

D

G

N

L

L

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Y

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V

A

C

A

G

N

G

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G

G

D

S

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E

I

K

S

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A

T

G

G

I

L

S

A

I

I

Y

H

H

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Y

F

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S

A

C

N

N

R

A

S

S

M

M

E

E

R

H

V

T

-

A

F

M

F

Y

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N

A

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D

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F

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|
G

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A

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Y

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I

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Y

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Q

L

L

P

P

D

D

L

L

G

G

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P

I

I

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A

N

N

G

G

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L

A

A

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N

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P

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R

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D

F

F

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A

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A

A

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D

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D

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C

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F

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D

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W

H

H

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N

N

Y

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V

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P

Y

Y

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K

Y

Y

D

D

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R

K

F

F

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F

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A

A

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A

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I

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F

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A

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M

F

F

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A

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F

F

G173 (= G168) mutation to R: Suppresses fah-1 RNAi-mediated toxicity.

1ey2A Human homogentisate dioxygenase with fe(ii) (see paper)
50% identity, 94% coverage: 10:418/434 of query aligns to 3:413/419 of 1ey2A

query
sites
1ey2A

L

L

V

K

Y

Y

Q

I

S

S

G

G

F

F

G

G

N

N

E

E

F

C

S

S

S

S

E

E

-

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-

P

A

R

L

C

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P

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G

A

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L

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E

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G

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Q

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N

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Q

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Y

G

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L

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Y

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E

E

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G

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A

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F

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C

A

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A

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D

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F

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D

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G

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L

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M

L

A

C

A

G

N

A

S

G

G

D

S

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E

K

K

S

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N

A

N

G

G

I

L

S

A

I

I

Y

H

H

I

Y

F

R

L

A

C

N

N

R

T

S

S

M

M

E

E

-

N

R

R

V

C

F

F

F

Y

N

N

A

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D

G

G

E

D

W

F

L

L

L

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V

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P

E

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G

G

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N

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E

L

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L

M

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N

E

E

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I

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C

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V

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G

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K

R

F

F

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I

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D

L

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L

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D

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E

A

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R

G

G

Y

Y

L

I

A

L

E

E

N

V

H

Y

G

G

A

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H

L

F

R

E

L

L

P

P

D

D

L

L

G

G

P

P

I

I

G

G

S

A

N

N

G

G

L

L

A

A

N

N

P

P

R

R

D

D

F

F

L

L

T

I

P

P

V

I

A

A

H

W

Y

Y

E

E

D

D

L

R

K

Q

Q

V

P

P

-

G

-

G

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Y

T

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L

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N

K

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G

G

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F

G

A

C

A

E

K

L

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D

D

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S

P

P

L

F

N

N

V

V

V

V

A

A

W

W

H

H

G

G

N

N

N

Y

V

T

P

P

Y

Y

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K

Y

Y

D

N

L

L

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R

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F

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A

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F

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|
H

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A

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D

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S

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I

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F

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V

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L

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A

N

I

L

A

D

D

F

F

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V

I

I

F

F

P

P

P

P

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R

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W

M

G

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V

A

A

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D

N

K

T

T

F

F

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R

P

P

P

P

W

Y

F

Y

H
|
H

R

R

N

N

L

C

M

M

N

S

E
|
E

F

F

M

M

G

G

L

L

I

I

Q

R

G

-

T

-

Y

-

D

-

A

-

K

-

A

-

E

-

G

G

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F

L

L

P

P

G

G

G

G

A

G

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|
H

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M

M

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H
|
H

G

G

P

P

D

D

G

A

E

D

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C

C

F

T

E

K

K

A

A

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K

A

V

E

K

L

L

A

A

P

P

S

E

K

R

I

I

-

A

D

D

N

G

T

T

M

M

A

A

F

F

M

M

F

F

E

E

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S

S

S

Q

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L

L

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A

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T

R

K

F

W

A

G

L

L

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A

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R

L

L

Q

K

N

S

N

H

Y

F

active site: H291 (= H289), H334 (= H332), E340 (= E338), H356 (= H362), H362 (= H368)
binding fe (ii) ion: H334 (= H332), E340 (= E338), H362 (= H368)

Query Sequence

>AO353_15550 FitnessBrowser__pseudo3_N2E3:AO353_15550
MNLDSTAPALVYQSGFGNEFSSEALPGALPVGQNSPQKAPYGLYTELFSGTAFTMARSEA
RRTWMYRIQPSANHPAFVKLERQLAGGPLGDVTPNRLRWNPLEIPGEPTDFIDGLVRMAA
NSGSEKPAGISIYHYRANRSMERVFFNADGEWLLVPEQGRLRIATELGVLELEPLEIAVL
PRGLKFRIELLDPQARGYLAENHGAPLRLPDLGPIGSNGLANPRDFLTPVAHYEDLKQPT
TLVQKFLGELWGCELDHSPLNVVAWHGNNVPYKYDLRRFNTIGTVSFDHPDPSIFTVLTS
PTSVHGLANLDFVIFPPRWMVAENTFRPPWFHRNLMNEFMGLIQGTYDAKAEGFLPGGAS
LHSCMSAHGPDGETCTKAINAELAPSKIDNTMAFMFETSQVLRPSRFALDCPQLQNNYDA
CWASLPVTFNPNRR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory