SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO353_21195 FitnessBrowser__pseudo3_N2E3:AO353_21195 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7bw9A Crystal structure of serine acetyltransferase isoform 3 in complex with cysteine from entamoeba histolytica
33% identity, 94% coverage: 3:165/174 of query aligns to 101:270/280 of 7bw9A

query
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7bw9A

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binding cysteine: D114 (= D15), P115 (≠ W16), D179 (= D77), H180 (= H78), A206 (≠ L104), R214 (≠ I111), H215 (≠ A112)

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
37% identity, 83% coverage: 19:162/174 of query aligns to 109:246/272 of 3gvdI

query
sites
3gvdI

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binding cysteine: D160 (= D77), G186 (= G103), G187 (vs. gap), R195 (≠ I111)

Sites not aligning to the query:

binding cysteine: 95, 96

8i09A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with butyl gallate (see paper)
36% identity, 83% coverage: 19:162/174 of query aligns to 105:242/246 of 8i09A

query
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8i09A

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binding cysteine: D156 (= D77), H157 (= H78), G164 (≠ S85), R191 (≠ I111), H192 (≠ A112)
binding butyl 3,4,5-tris(oxidanyl)benzoate: G183 (vs. gap), T184 (≠ L104), G202 (= G122), K218 (≠ V138), I219 (= I139), G220 (= G140), L226 (= L146), A236 (≠ V156)

Sites not aligning to the query:

binding cysteine: 91

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
36% identity, 83% coverage: 19:162/174 of query aligns to 102:239/258 of 8i04A

query
sites
8i04A

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binding serine: D153 (= D77), H154 (= H78), G180 (vs. gap), R188 (≠ I111), H189 (≠ A112)

Sites not aligning to the query:

binding serine: 88, 89

8i06A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with coa (see paper)
36% identity, 83% coverage: 19:162/174 of query aligns to 106:243/244 of 8i06A

query
sites
8i06A

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binding coenzyme a: H158 (= H78), L177 (≠ R97), Q178 (≠ N98), G203 (= G122), A204 (= A123), G221 (= G140), A222 (= A141), T235 (≠ V154), A237 (≠ V156)
binding cysteine: H158 (= H78)

Sites not aligning to the query:

binding cysteine: 93

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
34% identity, 83% coverage: 19:162/174 of query aligns to 98:232/233 of 4n6bA

query
sites
4n6bA

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binding coenzyme a: H150 (= H78), L169 (≠ R97), G176 (≠ L104), K208 (≠ V138), G210 (= G140), A211 (= A141), L216 (= L146), V226 (= V156)

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
35% identity, 83% coverage: 19:162/174 of query aligns to 106:243/262 of 1t3dA

query
sites
1t3dA

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binding cysteine: D157 (= D77), G184 (vs. gap), R192 (≠ I111), H193 (≠ A112)

Sites not aligning to the query:

binding cysteine: 92, 93

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
33% identity, 83% coverage: 19:162/174 of query aligns to 105:242/243 of 4n69A

query
sites
4n69A

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K

M

I

G

G

N

D

N

G

V

V

D

L

I

I

G

G

A

A

G

G

A

A

K

T

V

I

L

L

G

G

N

N

I

I

R

K

I

I

G

G

N

E

N

G

V

A

V

K

I

V

G

G

A

A

N

G

A

S

V

V

V

V

L

L

T

I

D

D

V

V

P

P

D

P

D

R

S

T

V

T

A

A

V

V

G

G

V

N

P

P

A

A

T

R

I

L

binding serine: G183 (vs. gap), R191 (≠ I111), H192 (≠ A112)

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
34% identity, 90% coverage: 5:160/174 of query aligns to 82:242/261 of 6wyeA

query
sites
6wyeA

I

V

R

E

A

A

D

D

L

L

R

K

A

A

-

I

Y

Y

A

E

G

R

D
|
D

W
x
P

A

A

A

C

Q

D

G

E

F

Y

W

S

V

L

M

P

L

L

V

L

Y

Y

R

-

F

F

G

K

R

G

W

F

R

H

Y

A

G

I

V

Q

R

A

P

H

A

R

F

I

L

N

R

H

K

R

L

L

F

Y

S

L

F

D

I

G

Y

R

K

K

V

T

L

L

F

A

K

Y

L

F

V

L

Q

Q

-

N

-

R

-

M

-

S

I

E

V

V

T

F

G

G

I

V

E

D

L

I

P

H

C

P

E

A

V

A

V

R

I

L

G

G

R

Y

N

G

F

L

V

M

I

L

D
|
D

H
|
H

F

A

G

T

G

G

I

F

V

V

I

A

S

G

G

E

Y

T

A

A

Q

V

F

L

G

G

D

N

D

N

C

I

R

S

I

I

R

L

N

H

G

G

V

V

V

T

V

L

G

G

L
x
G

K

S

N

G

V

K

D

E

E

G

-

G

P

D

I
x
R

A
x
H

P

P

V

K

M

I

G

G

N

D

N

G

V

V

D

M

I

I

G

G

A

A

G

N

A

A

K

S

V

I

L

L

G

G

N

N

I

I

R

R

I

I

G

G

N

S

N

N

V

A

V

K

I

I

G

G

A

A

N

G

A

S

V

V

V

V

L

V

T

S

D

D

V

V

P

P

D

P

D

S

S

I

V

T

A

V

V

V

G

G

V

V

P

P

A

A

binding (2S)-2-hydroxybutanedioic acid: D93 (= D15), P94 (≠ W16), D158 (= D77), H159 (= H78), G185 (≠ L104), R193 (≠ I111), H194 (≠ A112)

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
34% identity, 90% coverage: 5:160/174 of query aligns to 80:240/243 of 7ra4A

query
sites
7ra4A

I

V

R

E

A

A

D

D

L

L

R

K

A

A

-

I

Y

Y

A

E

G

R

D
|
D

W
x
P

A

A

A

C

Q

D

G

E

F

Y

W

S

V

L

M

P

L

L

V

L

Y

Y

R

-

F

F

G

K

R

G

W

F

R

H

Y

A

G

I

V

Q

R

A

P

H

A

R

F

I

L

N

R

H

K

R

L

L

F

Y

S

L

F

D

I

G

Y

R

K

K

V

T

L

L

F

A

K

Y

L

F

V

L

Q

Q

-

N

-

R

-

M

-

S

I

E

V

V

T

F

G

G

I

V

E

D

L

I

P

H

C

P

E

A

V

A

V

R

I

L

G

G

R

Y

N

G

F

L

V

M

I

L

D
|
D

H
|
H

F

A

G

T

G

G

I

F

V

V

I

A

S

G

G

E

Y

T

A

A

Q

V

F

L

G

G

D

N

D

N

C

I

R

S

I

I

R

L

N

H

G

G

V

V

V

T

V

L

G

G

L

G

K

S

N

G

V

K

D

E

E

G

-

G

P

D

I
x
R

A
x
H

P

P

V

K

M

I

G

G

N

D

N

G

V

V

D

M

I

I

G

G

A

A

G

N

A

A

K

S

V

I

L

L

G

G

N

N

I

I

R

R

I

I

G

G

N

S

N

N

V

A

V

K

I

I

G

G

A

A

N

G

A

S

V

V

V

V

L

V

T

S

D

D

V

V

P

P

D

P

D

S

S

I

V

T

A

V

V

V

G

G

V

V

P

P

A

A

binding serine: D91 (= D15), P92 (≠ W16), D156 (= D77), H157 (= H78), R191 (≠ I111), H192 (≠ A112)

1ssqD Serine acetyltransferase- complex with cysteine (see paper)
32% identity, 86% coverage: 19:168/174 of query aligns to 102:245/257 of 1ssqD

query
sites
1ssqD

Q

K

G

G

F

F

W

H

V

A

M

I

L

Q

V

S

Y

Y

R

R

F

I

G

T

R

H

W

Y

R

L

Y

W

G

N

V

Q

R

N

P

-

A

-

F

-

L

-

R

R

K

K

L

S

F

L

S

A

F

L

I

-

Y

-

K

-

V

Y

L

L

F

Q

K

N

L

Q

V

I

Q

S

I

V

V

A

T

F

G

D

I

V

E

D

L

I

P

H

C

P

E

A

V

A

V

K

I

I

G

G

R

H

N

G

F

I

V

M

I

F

D
|
D

H
|
H

F

A

G

T

G

G

I

I

V

V

I

V

S

G

G

E

Y

T

A

S

Q

V

F

I

G

E

D

N

D

D

C

V

R

S

I

I

R

L

N

Q

G

G

V

V

V

T

V

L

G

G

L

G

K

T

N

G

V

K

D

E

E

S

-

G

P

D

I

R

A

H

P

P

V

K

M

V

G

R

N

E

N

G

V

V

D

M

I

I

G

G

A

A

G

G

A

A

K

K

V

I

L

L

G

G

N

N

I

I

R

E

I

V

G

G

N

K

N

Y

V

A

V

K

I

I

G

G

A

A

N

N

A

S

V

V

V

V

L

L

T

N

D

P

V

V

P

P

D

E

D

Y

S

A

V

T

A

A

V

A

G

G

V

V

P

P

A

A

T

R

I

I

K

V

K

S

R

Q

Q

D

R

K

A

A

binding cysteine: D153 (= D77), H154 (= H78)

Sites not aligning to the query:

binding cysteine: 88

1sstA Serine acetyltransferase- complex with coa (see paper)
33% identity, 83% coverage: 19:162/174 of query aligns to 102:232/233 of 1sstA

query
sites
1sstA

Q

K

G

G

F

F

W

H

V

A

M

I

L

Q

V

S

Y

Y

R

R

F

I

G

T

R

H

W

Y

R

L

Y

W

G

N

V

Q

R

N

P

-

A

-

F

-

L

-

R

R

K

K

L

S

F

L

S

A

F

L

I

-

Y

-

K

-

V

Y

L

L

F

Q

K

N

L

Q

V

I

Q

S

I

V

V

A

T

F

G

D

I

V

E

D

L

I

P

H

C

P

E

A

V

A

V

K

I

I

G

G

R

H

N

G

F

I

V

M

I

F

D

D

H
|
H

F

A

G

T

G

G

I

I

V

V

I

V

S

G

G

E

Y

T

A

S

Q

V

F

I

G

E

D

N

D

D

C

V

R

S

I

I

R
x
L

N

Q

G

G

V

V

V

T

V

L

G

G

L
x
G

K
x
T

N

H

V

-

D

-

E

-

P

-

I

-

A

-

P

P

V

K

M

V

G

R

N

E

N

G

V

V

D

M

I

I

G

G

A
|
A

G

G

A

A

K

K

V

I

L
|
L

G

G

N

N

I

I

R

E

I

V

G

G

N

K

N

Y

V

A

V
x
K

I

I

G
|
G

A
|
A

N

N

A

S

V
|
V

V

V

L
|
L

T

N

D

P

V

V

P

P

D

E

D

Y

S

A

V
x
T

A

A

V
x
A

G

G

V
|
V

P
|
P

A

A

T

R

I

I

binding coenzyme a: H154 (= H78), L173 (≠ R97), G180 (≠ L104), T181 (≠ K105), A193 (= A123), L198 (= L128), K208 (≠ V138), G210 (= G140), A211 (= A141), V214 (= V144), L216 (= L146), T224 (≠ V154), A226 (≠ V156), V228 (= V158), P229 (= P159)

Sites not aligning to the query:

binding coenzyme a: 233

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
32% identity, 87% coverage: 19:169/174 of query aligns to 102:246/258 of 4h7oA

query
sites
4h7oA

Q

K

G

G

F

Y

W

H

V

A

M

L

L

Q

V

G

Y

Y

R

R

F

V

G

A

R

N

W

W

-

L

-

W

R

R

Y

Q

G

G

V

-

R

-

P

-

A

-

F

-

L

-

R

R

K

K

L

A

F

L

S

A

F

T

I
x
Y

Y

F

K

Q

V

-

L

-

F

-

K

N

L
x
Q

V

I

Q

S

I

V

V

A

T

C

G

Q

I

V

E

D

L

I

P

H

C

P

E

A

V

A

V

R

I

I

G

G

R

R

N

G

F

I

V

M

I

L

D
|
D

H
|
H

F

A

G

T

G

G

I

I

V

V

I

I

S

G

G

E

Y

T

A

A

Q

V

F

V

G

E

D

D

D

D

C

V

R

S

I

I

R

L

N

Q

G

D

V

V

V

T

V

L

G

G

L
x
G

K

T

N

G

V

K

D

E

-

C

E

G

P

D

I
x
R

A
x
H

P

P

V

K

M

I

G

R

N

E

N

G

V

V

D

M

I

I

G

G

A

A

G

G

A

A

K

K

V

I

L

L

G

G

N

N

I

I

R

E

I

V

G

G

N

E

N

G

V

A

V

K

I

I

G

G

A

S

N

G

A

S

V

V

V

V

L

L

T

Q

D

A

V

V

P

P

D

P

D

H

S

T

V

T

A

V

V

A

G

G

V

V

P

P

A

A

T

R

I

I

K

V

K

G

R

R

Q

P

R

Q

A

S

E

D

binding arginine: Y127 (≠ I48), Q131 (≠ L55)
binding cysteine: D153 (= D77), H154 (= H78), G180 (≠ L104), R188 (≠ I111), H189 (≠ A112)

Sites not aligning to the query:

3p47A Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-cysteine (see paper)
33% identity, 87% coverage: 4:154/174 of query aligns to 104:267/270 of 3p47A

query
sites
3p47A

N

T

I

L

R

K

A

T

D

D

L

L

-

I

R

A

A

A

Y

Y

A

A

G

G

D

D

W

P

A

A

A

A

Q

P

G

G

-

L

-

S

-

L

-

I

-

I

-

R

-

C

-

Y

-

P

-

G

F

F

W

Q

V

A

M

V

L

I

V

V

Y

Y

R

R

F

I

G

A

R

H

W

V

R

L

Y

Y

-

E

-

C

G

G

V

E

R

R

P

-

A

-

F

-

L

-

R

-

K

-

L

-

F

-

S

-

F

Y

I

Y

Y

C

K

R

V

E

L

M

F

M

K

E

L

S

V

V

Q

H

I

S

V

Y

T

T

G

S

I

I

E

D

L

I

P

H

C

P

E

G

V

A

V

S

I

I

G

K

R

G

N

H

F

F

V

F

I

I

D

D

H

H

F

G

G

V

G

G

I

V

V

V

I

I

S

G

G

E

Y

T

A

A

Q

I

F

I

G

G

D

E

D

W

C

C

R

R

I

I

R

Y

N

Q

G

S

V

V

V

T

V

L

G

G

L
x
A

K

M

N
x
H

V

F

D

Q

E

E

P

E

-

G

-

G

-

V

-

I

-

K

-

R

-

G

-

T

-

K

I
x
R

A
x
H

P

P

V

T

M

V

G

G

N

D

N

Y

V

V

D

T

I

I

G

G

A

T

G

G

A

A

K

K

V

V

L

L

G

G

N

N

I

I

R

I

I

V

G

G

N

S

N

H

V

V

V

R

I

I

G

G

A

A

N

N

A

C

V

W

V

I

L

D

T

R

D

D

V

V

P

D

D

S

D

N

S

Q

V

T

binding cysteine: A208 (≠ L104), H210 (≠ N106), R224 (≠ I111), H225 (≠ A112)

3q1xA Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-serine (see paper)
33% identity, 87% coverage: 4:154/174 of query aligns to 102:265/267 of 3q1xA

query
sites
3q1xA

N

T

I

L

R

K

A

T

D

D

L

L

-

I

R

A

A

A

Y

Y

A

A

G

G

D

D

W

P

A

A

A

A

Q

P

G

G

-

L

-

S

-

L

-

I

-

I

-

R

-

C

-

Y

-

P

-

G

F

F

W

Q

V

A

M

V

L

I

V

V

Y

Y

R

R

F

I

G

A

R

H

W

V

R

L

Y

Y

-

E

-

C

G

G

V

E

R

R

P

-

A

-

F

-

L

-

R

-

K

-

L

-

F

-

S

-

F

Y

I

Y

Y

C

K

R

V

E

L

M

F

M

K

E

L

S

V

V

Q

H

I

S

V

Y

T

T

G

S

I

I

E

D

L

I

P

H

C

P

E

G

V

A

V

S

I

I

G

K

R

G

N

H

F

F

V

F

I

I

D

D

H

H

F

G

G

V

G

G

I

V

V

V

I

I

S

G

G

E

Y

T

A

A

Q

I

F

I

G

G

D

E

D

W

C

C

R

R

I

I

R

Y

N

Q

G

S

V

V

V

T

V

L

G

G

L

A

K

M

N
x
H

V

F

D

Q

E

E

P

E

-

G

-

G

-

V

-

I

-

K

-

R

-

G

-

T

-

K

I
x
R

A
x
H

P

P

V

T

M

V

G

G

N

D

N

Y

V

V

D

T

I

I

G

G

A

T

G

G

A

A

K

K

V

V

L

L

G

G

N

N

I

I

R

I

I

V

G

G

N

S

N

H

V

V

V

R

I

I

G

G

A

A

N

N

A

C

V

W

V

I

L

D

T

R

D

D

V

V

P

D

D

S

D

N

S

Q

V

T

binding serine: H208 (≠ N106), R222 (≠ I111), H223 (≠ A112)

4hzdA Crystal structure of serine acetyltransferase in complex with coenzyme a from brucella abortus strain s19 (see paper)
31% identity, 83% coverage: 19:162/174 of query aligns to 106:243/250 of 4hzdA

query
sites
4hzdA

Q

K

G

G

F

F

W

H

V

A

M

I

L

Q

V

T

Y

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binding coenzyme a: H158 (= H78), G203 (= G122), A204 (= A123), A221 (≠ G140), A222 (= A141), T235 (≠ V154), A237 (≠ V156)
binding magnesium ion: S224 (≠ A143), A237 (≠ V156), G238 (= G157)

Sites not aligning to the query:

binding coenzyme a: 244

5tyhA Pgld from campylobacter jejuni nctc 11168 in complex with 5-(2- furanyl)-1h-pyrazole-3-carboxylic acid (see paper)
30% identity, 72% coverage: 42:166/174 of query aligns to 52:171/185 of 5tyhA

query
sites
5tyhA

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binding 3-(furan-2-yl)-1H-pyrazole-5-carboxylic acid: I75 (= I70), S76 (≠ G71), N96 (≠ G91), N108 (≠ G103), S126 (≠ G122), V127 (≠ A123), F142 (≠ V138), G144 (= G140), I145 (≠ A141), G163 (≠ V158)

5t2yA Crystal structure of c. Jejuni pgld in complex with 5-methyl-4- (methylamino)-2-phenethylthieno[2,3-d]pyrimidine-6-carboxylic acid (see paper)
30% identity, 72% coverage: 42:166/174 of query aligns to 59:178/192 of 5t2yA

query
sites
5t2yA

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binding 5-methyl-4-(methylamino)-2-(2-phenylethyl)thieno[2,3-d]pyrimidine-6-carboxylic acid: S133 (≠ G122), F149 (≠ V138), G151 (= G140), I152 (≠ A141), G170 (≠ V158)

Sites not aligning to the query:

binding 5-methyl-4-(methylamino)-2-(2-phenylethyl)thieno[2,3-d]pyrimidine-6-carboxylic acid: 185

3bsyA Pgld from campylobacter jejuni, nctc 11168, in complex with acetyl coenzyme a (see paper)
30% identity, 72% coverage: 42:166/174 of query aligns to 60:179/193 of 3bsyA

query
sites
3bsyA

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binding acetyl coenzyme *a: H132 (≠ D120), S134 (≠ G122), V135 (≠ A123), A140 (≠ L128), F150 (≠ V138), G152 (= G140), I153 (≠ A141), L158 (= L146), G170 (= G157), G171 (≠ V158), V176 (≠ K163)

Sites not aligning to the query:

binding acetyl coenzyme *a: 186, 189

3bssA Pgld from campylobacter jejuni, nctc 11168, with native substrate (see paper)
30% identity, 72% coverage: 42:166/174 of query aligns to 61:180/194 of 3bssA

query
sites
3bssA

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Sites not aligning to the query:

binding UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose: 12, 14, 34, 35, 36, 54, 55, 59

Query Sequence

>AO353_21195 FitnessBrowser__pseudo3_N2E3:AO353_21195
MFENIRADLRAYAGDWAAQGFWVMLVYRFGRWRYGVRPAFLRKLFSFIYKVLFKLVQIVT
GIELPCEVVIGRNFVIDHFGGIVISGYAQFGDDCRIRNGVVVGLKNVDEPIAPVMGNNVD
IGAGAKVLGNIRIGNNVVIGANAVVLTDVPDDSVAVGVPATIKKRQRAEARECL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory