SitesBLAST
Comparing AO353_23545 FitnessBrowser__pseudo3_N2E3:AO353_23545 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1krhA X-ray structure of benzoate dioxygenase reductase (see paper)
54% identity, 99% coverage: 2:335/337 of query aligns to 2:337/337 of 1krhA
- active site: C306 (= C304)
- binding flavin-adenine dinucleotide: Y143 (= Y141), R155 (= R153), S156 (≠ A154), Y157 (= Y155), S158 (= S156), V171 (≠ L169), V172 (≠ I170), G178 (= G176), K179 (≠ L177), M180 (= M178), S181 (= S179), T219 (= T217), E332 (= E330), F334 (= F332), S335 (≠ A333), A336 (= A334), N337 (≠ S335)
- binding fe2/s2 (inorganic) cluster: M38 (≠ L38), D39 (= D39), C40 (= C40), R41 (= R41), G43 (= G43), C45 (= C45), G46 (= G46), C48 (= C48), C82 (= C81)
5ogxA Crystal structure of amycolatopsis cytochrome p450 reductase gcob. (see paper)
32% identity, 96% coverage: 15:336/337 of query aligns to 9:333/333 of 5ogxA
- binding flavin-adenine dinucleotide: Y132 (= Y141), R144 (= R153), Q145 (≠ A154), Y146 (= Y155), S147 (= S156), H161 (vs. gap), V162 (≠ I170), V165 (= V173), G168 (= G176), V169 (≠ L177), A170 (≠ M178), T171 (≠ S179), T214 (= T217), F329 (= F332), P331 (≠ A334), S333 (≠ A336)
- binding fe2/s2 (inorganic) cluster: C34 (= C40), N35 (≠ R41), G37 (= G43), C39 (= C45), G40 (= G46), C42 (= C48), C74 (= C81)
P0DPQ8 Aromatic O-demethylase, reductase subunit; NADH--hemoprotein reductase; EC 1.6.2.- from Amycolatopsis sp. (strain ATCC 39116 / 75iv2) (see paper)
32% identity, 96% coverage: 15:336/337 of query aligns to 10:334/334 of P0DPQ8
- C35 (= C40) binding [2Fe-2S] cluster
- C40 (= C45) binding [2Fe-2S] cluster
- C43 (= C48) binding [2Fe-2S] cluster
- C75 (= C81) binding [2Fe-2S] cluster
- RQYS 145:148 (≠ RAYS 153:156) binding FAD
- HVR 162:164 (≠ -IR 170:171) binding FAD
- VAT 170:172 (≠ LMS 177:179) binding FAD
- T215 (= T217) binding FAD
- F330 (= F332) binding FAD
- S334 (≠ A336) binding FAD
1tvcA Fad and nadh binding domain of methane monooxygenase reductase from methylococcus capsulatus (bath) (see paper)
33% identity, 61% coverage: 134:337/337 of query aligns to 42:249/250 of 1tvcA
- active site: Y63 (= Y155), S64 (= S156), L215 (= L303)
- binding dihydroflavine-adenine dinucleotide: F49 (≠ Y141), R61 (= R153), S62 (≠ A154), Y63 (= Y155), S64 (= S156), L78 (= L169), I79 (= I170), R80 (= R171), L82 (≠ V173), F87 (≠ M178), G128 (= G218), N155 (≠ T245), E159 (≠ D249), S186 (vs. gap), G187 (vs. gap), E242 (= E330), F244 (= F332), L245 (≠ A333)
Sites not aligning to the query:
4wqmA Structure of the toluene 4-monooxygenase nadh oxidoreductase t4mof, k270s k271s variant (see paper)
28% identity, 96% coverage: 11:332/337 of query aligns to 7:325/326 of 4wqmA
- binding flavin-adenine dinucleotide: C36 (= C40), S38 (≠ D42), W57 (≠ I64), Y135 (= Y141), R146 (= R153), A147 (= A154), Y148 (= Y155), S149 (= S156), I162 (≠ L169), V163 (≠ I170), G169 (= G176), K170 (≠ L177), V171 (≠ M178), S172 (= S179), S209 (≠ T217), F325 (= F332)
- binding fe2/s2 (inorganic) cluster: Y34 (≠ L38), C36 (= C40), N37 (≠ R41), G39 (= G43), C41 (= C45), G42 (= G46), C44 (= C48), C76 (= C81)
Q03304 Toluene-4-monooxygenase system, ferredoxin--NAD(+) reductase component; T4MO; Ferredoxin--NAD(+) reductase; Toluene-4-monooxygenase systme, electron transfer component; EC 1.18.1.3 from Ectopseudomonas mendocina (Pseudomonas mendocina) (see paper)
28% identity, 96% coverage: 11:332/337 of query aligns to 7:325/326 of Q03304
- C36 (= C40) binding [2Fe-2S] cluster
- C41 (= C45) binding [2Fe-2S] cluster
- C44 (= C48) binding [2Fe-2S] cluster
- C76 (= C81) binding [2Fe-2S] cluster
- RAYS 146:149 (= RAYS 153:156) binding FAD
- IVK 162:164 (≠ LIR 169:171) binding FAD
- KVS 170:172 (≠ LMS 177:179) binding FAD
7c3bC Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
28% identity, 91% coverage: 28:332/337 of query aligns to 28:333/334 of 7c3bC
7c3aA Ferredoxin reductase in carbazole 1,9a-dioxygenase (see paper)
28% identity, 91% coverage: 28:332/337 of query aligns to 27:320/321 of 7c3aA
- binding flavin-adenine dinucleotide: C39 (= C40), S41 (≠ D42), W60 (vs. gap), R152 (= R153), A153 (= A154), Y154 (= Y155), S155 (= S156), Y169 (≠ L169), I170 (= I170), G176 (= G176), R177 (≠ L177), F178 (≠ M178), S179 (= S179), A218 (≠ T217), F320 (= F332)
- binding fe2/s2 (inorganic) cluster: Y37 (≠ L38), C39 (= C40), A40 (≠ R41), G42 (= G43), C44 (= C45), G45 (= G46), C47 (= C48), C80 (= C81)
Sites not aligning to the query:
7c3bB Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
27% identity, 91% coverage: 28:332/337 of query aligns to 27:305/306 of 7c3bB
7qu5A X-ray structure of fad domain of nqrf of pseudomonas aeruginosa (see paper)
27% identity, 54% coverage: 153:334/337 of query aligns to 83:280/280 of 7qu5A
- binding flavin-adenine dinucleotide: R83 (= R153), A84 (= A154), Y85 (= Y155), S86 (= S156), N100 (≠ L169), A104 (vs. gap), L112 (≠ V173), P113 (= P174), G115 (= G176), Q116 (≠ L177), M117 (= M178), S118 (= S179), A155 (≠ T217), F278 (= F332)
- binding gamma-Valerolactone: S105 (vs. gap), P108 (vs. gap), D196 (≠ Q257), W207 (≠ F268), H208 (≠ S269), L209 (≠ A270)
Sites not aligning to the query:
7qu3A X-ray structure of fad domain of nqrf of pseudomonas aeruginosa (see paper)
27% identity, 54% coverage: 153:333/337 of query aligns to 83:279/279 of 7qu3A
- binding flavin-adenine dinucleotide: R83 (= R153), A84 (= A154), Y85 (= Y155), S86 (= S156), N100 (≠ L169), A104 (vs. gap), S105 (vs. gap), L112 (≠ V173), P113 (= P174), G115 (= G176), Q116 (≠ L177), M117 (= M178), S118 (= S179), A155 (≠ T217), F278 (= F332)
- binding 4-(benzimidazol-1-ylmethyl)benzenecarbonitrile: R102 (= R171), G154 (= G216), G182 (= G243), F226 (≠ Y284), I227 (≠ V285), P252 (= P306), M255 (= M309)
Sites not aligning to the query:
4u9uA Crystal structure of nqrf fad-binding domain from vibrio cholerae (see paper)
27% identity, 47% coverage: 176:334/337 of query aligns to 114:279/279 of 4u9uA
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 38, 81, 82, 83, 84, 98, 102, 103, 104, 111, 112
- binding magnesium ion: 14, 59
8a1uF Sodium pumping nadh-quinone oxidoreductase with substrates nadh and q2 (see paper)
27% identity, 47% coverage: 176:332/337 of query aligns to 242:405/405 of 8a1uF
- binding flavin-adenine dinucleotide: G242 (= G176), Q243 (≠ L177), M244 (= M178), A282 (≠ T217), F405 (= F332)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G281 (= G216), A282 (≠ T217), G309 (= G243), R311 (≠ T245), S339 (≠ A273), F353 (vs. gap), C377 (= C304), G378 (= G305), P379 (= P306), M381 (≠ P308), M382 (= M309), D403 (≠ E330)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 166, 209, 210, 211, 212, 230, 233, 239, 240
- binding fe2/s2 (inorganic) cluster: 69, 71, 73, 75, 76, 78, 110
- binding 1,4-dihydronicotinamide adenine dinucleotide: 212, 228
8a1yF Sodium pumping nadh-quinone oxidoreductase with inhibitor hqno (see paper)
27% identity, 47% coverage: 176:334/337 of query aligns to 243:408/408 of 8a1yF
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 210, 211, 212, 213, 228, 229, 231, 240, 241
- binding fe2/s2 (inorganic) cluster: 70, 74, 76, 77, 79, 111
A5F5Y4 Na(+)-translocating NADH-quinone reductase subunit F; Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F; EC 7.2.1.1 from Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) (see paper)
27% identity, 47% coverage: 176:334/337 of query aligns to 243:408/408 of A5F5Y4
- S246 (= S179) mutation to A: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
Sites not aligning to the query:
- 70 binding [2Fe-2S] cluster; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
- 76 binding [2Fe-2S] cluster; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
- 79 binding [2Fe-2S] cluster; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
- 111 binding [2Fe-2S] cluster; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
- 210 R→L: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
- 212 Y→L: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
7romA Crystal structure of saccharomyces cerevisiae nadh-cytochrome b5 reductase 1 (cbr1) fragment (residues 28-284) bound to fad
31% identity, 53% coverage: 139:315/337 of query aligns to 45:232/255 of 7romA
- binding flavin-adenine dinucleotide: R61 (= R153), S62 (≠ A154), Y63 (= Y155), T64 (≠ S156), L79 (= L169), V80 (≠ I170), Y83 (≠ V173), T85 (≠ G175), G86 (= G176), N87 (≠ L177), V88 (≠ M178), S89 (= S179), T127 (= T217), A130 (= A220)
2r6hC Crystal structure of the domain comprising the NAD binding and the fad binding regions of the nadh:ubiquinone oxidoreductase, na translocating, f subunit from porphyromonas gingivalis
26% identity, 55% coverage: 150:333/337 of query aligns to 83:289/289 of 2r6hC
- active site: C260 (= C304)
- binding flavin-adenine dinucleotide: R86 (= R153), A87 (= A154), Y88 (= Y155), S89 (= S156), N103 (vs. gap), A107 (vs. gap), T108 (vs. gap), I122 (≠ V173), K123 (≠ P174), P124 (≠ G175), G125 (= G176), I126 (≠ L177), S127 (≠ M178), S128 (= S179), F288 (= F332)
Sites not aligning to the query:
7qu0A X-ray structure of fad domain of nqrf of klebsiella pneumoniae (see paper)
26% identity, 47% coverage: 176:332/337 of query aligns to 116:279/279 of 7qu0A
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 40, 83, 84, 85, 86, 104, 105, 106, 107, 113, 114, 115
7qtyA X-ray structure of fad domain of nqrf of klebsiella pneumoniae (see paper)
26% identity, 47% coverage: 176:332/337 of query aligns to 116:279/279 of 7qtyA
- binding 1-(furan-2-ylmethyl)-3-(2-methylphenyl)thiourea: A184 (≠ V244), R185 (≠ T245), S213 (≠ A273), F227 (≠ Y284), H229 (≠ T286), V255 (≠ P308), M256 (= M309)
- binding flavin-adenine dinucleotide: G116 (= G176), I117 (≠ L177), M118 (= M178), S119 (= S179), F279 (= F332)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 40, 83, 84, 85, 86, 100, 104, 105, 107, 113, 114
3ozwA The crystal structure of flavohemoglobin from r. Eutrophus in complex with ketoconazole (see paper)
29% identity, 58% coverage: 136:332/337 of query aligns to 185:396/403 of 3ozwA
- binding flavin-adenine dinucleotide: R206 (= R153), Q207 (≠ A154), Y208 (= Y155), S209 (= S156), S222 (≠ L169), V223 (≠ I170), K224 (≠ R171), E226 (vs. gap), P232 (= P174), G234 (= G176), Y235 (≠ L177), V236 (≠ M178), S237 (= S179), V276 (≠ T217), T279 (≠ A220), V395 (≠ K331), F396 (= F332)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 46, 50
- binding protoporphyrin ix containing fe: 42, 43, 81, 85, 88, 90, 94, 95, 98, 126, 130, 133
- binding 1-acetyl-4-(4-{[(2R,4S)-2-(2,4-dichlorophenyl)-2-(1H-imidazol-1-ylmethyl)-1,3-dioxolan-4-yl]methoxy}phenyl)piperazine: 53, 56, 102, 398
Query Sequence
>AO353_23545 FitnessBrowser__pseudo3_N2E3:AO353_23545
MTHSIALNFEDGVTRFVDANPGETVADAAYRQGINIPLDCRDGACGTCKCFAEAGRYDLG
EEYIEDALSSDEAEKGFVLTCQMRALSDCVVRVPVSSDVCRTQKAHYSAAISQVRQLSDS
TIALSIKGEALSKLAFLPGQYVNLGIPGSEQTRAYSFSSLQRDGEVSFLIRNVPGGLMSS
FLTGIAKAGDSMSLAGPLGSFYLRDIQRPLLLLAGGTGLAPFTAMLEKIAEQGSEYPLHL
IYGVTNDFDLVELDRLQALAARIPNFSFSACVANPQSAHPLKGYVTQHIEPKHLNDGDVD
VYLCGPPPMVEAVSQFIREQGVSPKNFYYEKFAASAA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory