SitesBLAST
Comparing AO353_24330 FitnessBrowser__pseudo3_N2E3:AO353_24330 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1cl1B Cystathionine beta-lyase (cbl) from escherichia coli (see paper)
40% identity, 98% coverage: 5:384/389 of query aligns to 1:391/392 of 1cl1B
P06721 Cystathionine beta-lyase MetC; CBL; CL; Beta-cystathionase MetC; Cysteine desulfhydrase MetC; CD; Cysteine lyase MetC; Cysteine-S-conjugate beta-lyase MetC; EC 4.4.1.13; EC 4.4.1.28 from Escherichia coli (strain K12) (see 2 papers)
40% identity, 98% coverage: 5:384/389 of query aligns to 4:394/395 of P06721
- K210 (= K204) modified: N6-(pyridoxal phosphate)lysine
2gqnA Cystathionine beta-lyase (cbl) from escherichia coli in complex with n-hydrazinocarbonylmethyl-2-nitro-benzamide (see paper)
41% identity, 93% coverage: 23:384/389 of query aligns to 18:390/391 of 2gqnA
- active site: R54 (= R54), Y107 (= Y107), D181 (= D179), K206 (= K204)
- binding (5-hydroxy-6-methyl-4-((2-(2-(2-nitrobenzamido)acetyl)hydrazinyl)methyl)pyridin-3-yl)methyl dihydrogen phosphate: C81 (≠ S81), G82 (= G82), A83 (≠ L83), Y107 (= Y107), E108 (≠ S108), D181 (= D179), A203 (= A201), T205 (= T203), K206 (= K204), M215 (≠ V213), Y334 (= Y331), S335 (= S332), W336 (= W333), R368 (= R362)
2fq6A Cystathionine beta-lyase (cbl) from escherichia coli in complex with n-hydrazinocarbonylmethyl-2-trifluoromethyl-benzamide (see paper)
41% identity, 93% coverage: 23:384/389 of query aligns to 18:390/391 of 2fq6A
- active site: R54 (= R54), Y107 (= Y107), D181 (= D179), K206 (= K204)
- binding phosphoric acid mono-(5-hydroxy-6-methyl-4-{[2-(2-trifluoromethyl-benzoylamino)-acetyl]-hydrazonomethyl}-pyridin-3-ylmethyl)ester: C81 (≠ S81), G82 (= G82), A83 (≠ L83), Y107 (= Y107), P109 (= P109), D181 (= D179), A203 (= A201), T205 (= T203), K206 (= K204), M215 (≠ V213), Y334 (= Y331), S335 (= S332), W336 (= W333), R368 (= R362)
1cl2A Cystathionine beta-lyase (cbl) from escherichia coli in complex with aminoethoxyvinylglycine (see paper)
41% identity, 93% coverage: 23:384/389 of query aligns to 18:390/391 of 1cl2A
- active site: R54 (= R54), Y107 (= Y107), D181 (= D179), K206 (= K204)
- binding (2E,3E)-4-(2-aminoethoxy)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: C81 (≠ S81), G82 (= G82), A83 (≠ L83), Y107 (= Y107), D181 (= D179), A203 (= A201), T205 (= T203), K206 (= K204), M215 (≠ V213), Y334 (= Y331), S335 (= S332), W336 (= W333), R368 (= R362)
8sabA Crystal structure of cystathionine beta lyase from klebsiella aerogenes, plp adduct with alanine (c2 form)
40% identity, 98% coverage: 5:384/389 of query aligns to 1:391/392 of 8sabA
- binding lysine: N9 (≠ H13), R12 (≠ I16), R13 (≠ E17), K14 (≠ P18), T17 (≠ H21), L330 (= L326), E341 (= E337)
- binding pyridoxal-5'-phosphate: C82 (≠ S81), G83 (= G82), A84 (≠ L83), Y108 (= Y107), D182 (= D179), A204 (= A201), T206 (= T203), K207 (= K204), M216 (≠ V213), W337 (= W333)
- binding alanyl-pyridoxal-5'-phosphate: C82 (≠ S81), G83 (= G82), A84 (≠ L83), Y108 (= Y107), D182 (= D179), A204 (= A201), T206 (= T203), K207 (= K204), M216 (≠ V213), Y335 (= Y331), S336 (= S332), W337 (= W333), R369 (= R362)
8sadA Crystal structure of cystathionine beta lyase from klebsiella aerogenes, plp/malonate complex (c2 form)
40% identity, 98% coverage: 5:384/389 of query aligns to 7:397/398 of 8sadA
- binding magnesium ion: A359 (vs. gap), R362 (= R349), A365 (≠ T352)
- binding pyridoxal-5'-phosphate: C88 (≠ S81), G89 (= G82), A90 (≠ L83), Y114 (= Y107), D188 (= D179), A210 (= A201), T212 (= T203), K213 (= K204), M222 (≠ V213), W343 (= W333)
4itxA P113s mutant of e. Coli cystathionine beta-lyase metc inhibited by reaction with l-ala-p (see paper)
41% identity, 93% coverage: 23:384/389 of query aligns to 18:390/391 of 4itxA
- active site: R54 (= R54), Y107 (= Y107), D181 (= D179), K206 (= K204)
- binding {1-[(3-hydroxy-methyl-5-phosphonooxy-methyl-pyridin-4-ylmethyl)-amino]-ethyl}-phosphonic acid: C81 (≠ S81), G82 (= G82), A83 (≠ L83), Y107 (= Y107), D181 (= D179), A203 (= A201), T205 (= T203), K206 (= K204), M215 (≠ V213), Y334 (= Y331), S335 (= S332), W336 (= W333), R368 (= R362)
8u99A Crystal structure of cystathionine beta lyase from klebsiella aerogenes (plp-serine adduct)
40% identity, 97% coverage: 6:384/389 of query aligns to 1:390/391 of 8u99A
- binding pyridoxal-5'-phosphate: C81 (≠ S81), G82 (= G82), A83 (≠ L83), Y107 (= Y107), D181 (= D179), T205 (= T203), K206 (= K204), M215 (≠ V213), W336 (= W333)
- binding serine: Y107 (= Y107), K206 (= K204), Y334 (= Y331), S335 (= S332), W336 (= W333), R368 (= R362)
8u98A Crystal structure of cystathionine beta lyase from klebsiella aerogenes (plp-glycine adduct)
40% identity, 97% coverage: 6:384/389 of query aligns to 1:390/391 of 8u98A
- binding glycine: Y107 (= Y107), K206 (= K204), Y334 (= Y331), S335 (= S332), W336 (= W333), R368 (= R362)
- binding pyridoxal-5'-phosphate: Y52 (= Y52), R54 (= R54), C81 (≠ S81), G82 (= G82), A83 (≠ L83), Y107 (= Y107), D181 (= D179), A203 (= A201), T205 (= T203), K206 (= K204), M215 (≠ V213), W336 (= W333)
8sa9A Crystal structure of cystathionine beta lyase from klebsiella aerogenes, plp-oxamate adduct (c2 form)
40% identity, 97% coverage: 6:384/389 of query aligns to 1:390/391 of 8sa9A
- binding pyridoxal-5'-phosphate: C81 (≠ S81), G82 (= G82), A83 (≠ L83), Y107 (= Y107), D181 (= D179), A203 (= A201), T205 (= T203), K206 (= K204), M215 (≠ V213), W336 (= W333)
- binding [({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino](oxo)acetic acid: C81 (≠ S81), G82 (= G82), A83 (≠ L83), Y107 (= Y107), D181 (= D179), A203 (= A201), T205 (= T203), K206 (= K204), M215 (≠ V213), S335 (= S332), W336 (= W333), R368 (= R362)
4l0oH Structure determination of cystathionine gamma-synthase from helicobacter pylori
40% identity, 97% coverage: 7:384/389 of query aligns to 3:373/373 of 4l0oH
- active site: R40 (= R54), Y92 (= Y107), D164 (= D179), K189 (= K204)
- binding pyridoxal-5'-phosphate: Y38 (= Y52), R40 (= R54), S67 (= S81), G68 (= G82), L69 (= L83), Y92 (= Y107), D164 (= D179), S186 (≠ A201), T188 (= T203), K189 (= K204)
8j6nA Crystal structure of cystathionine gamma-lyase in complex with compound 1 (see paper)
37% identity, 85% coverage: 50:381/389 of query aligns to 49:385/390 of 8j6nA
- binding [6-methyl-4-[(~{E})-(oxamoylhydrazinylidene)methyl]-5-oxidanyl-pyridin-3-yl]methyl dihydrogen phosphate: Y51 (= Y52), R53 (= R54), G81 (= G82), L82 (= L83), Y105 (= Y107), E148 (= E150), N152 (≠ S154), D178 (= D179), S200 (≠ A201), T202 (= T203), K203 (= K204), E330 (≠ Y331), S331 (= S332), T346 (≠ I342), R366 (= R362)
4omaA The crystal structure of methionine gamma-lyase from citrobacter freundii in complex with l-cycloserine pyridoxal-5'-phosphate (see paper)
34% identity, 97% coverage: 8:383/389 of query aligns to 9:394/396 of 4omaA
- active site: R59 (= R54), Y112 (= Y107), D184 (= D179), K209 (= K204)
- binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: G87 (= G82), I88 (≠ L83), Y112 (= Y107), D184 (= D179), S206 (≠ A201), T208 (= T203), K209 (= K204), V337 (≠ Y331), S338 (= S332), R373 (= R362)
3jwbA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with norleucine (see paper)
34% identity, 97% coverage: 8:383/389 of query aligns to 9:394/396 of 3jwbA
3jwaA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with methionine phosphinate (see paper)
34% identity, 97% coverage: 8:383/389 of query aligns to 9:394/396 of 3jwaA
3jw9A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with s-ethyl-cysteine (see paper)
34% identity, 97% coverage: 8:383/389 of query aligns to 9:394/396 of 3jw9A
6egrA Crystal structure of citrobacter freundii methionine gamma-lyase with v358y replacement (see paper)
34% identity, 97% coverage: 8:383/389 of query aligns to 9:394/396 of 6egrA
5m3zA Crystal structure of citrobacter freundii methionine gamma-lyase with c115h replacement in the complex with l-norleucine (see paper)
34% identity, 97% coverage: 8:383/389 of query aligns to 8:393/395 of 5m3zA
- active site: R58 (= R54), Y111 (= Y107), D183 (= D179), K208 (= K204)
- binding norleucine: Y111 (= Y107), H113 (≠ P109), K208 (= K204), V336 (≠ Y331), S337 (= S332)
- binding pyridoxal-5'-phosphate: G86 (= G82), I87 (≠ L83), Y111 (= Y107), E154 (= E150), D183 (= D179), T185 (= T181), S205 (≠ A201), T207 (= T203), K208 (= K204)
- binding 2-[o-phosphonopyridoxyl]-amino-hexanoic acid: G86 (= G82), I87 (≠ L83), Y111 (= Y107), D183 (= D179), S205 (≠ A201), T207 (= T203), K208 (= K204), V336 (≠ Y331), S337 (= S332), R372 (= R362)
4hf8A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with glycine (see paper)
34% identity, 97% coverage: 8:383/389 of query aligns to 9:394/396 of 4hf8A
- active site: R59 (= R54), Y112 (= Y107), D184 (= D179), K209 (= K204)
- binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: G87 (= G82), I88 (≠ L83), Y112 (= Y107), E155 (= E150), N159 (≠ S154), D184 (= D179), S206 (≠ A201), K209 (= K204), S338 (= S332), R373 (= R362)
Query Sequence
>AO353_24330 FitnessBrowser__pseudo3_N2E3:AO353_24330
MKKYKNVQTLLAHASIEPDQHHGFVNTPVYRGSTVAFPTCESMREGRQKYTYGRWNNPST
EALTQALQQLEGAEGTVLCPSGLSACTTAILSVVGAGDHLLIADNVYSPIRAFCEQVGQR
LGIEVTFYDPTIGAGIVDFLKPNTKAIYTESPGSLTLEIQDIPAIAKVAHEHDILVITDN
TWGTPLYFPSLELGVDLSIMAATKYIVGHSDAVLGTVSASKRAWDSLKRFHFNMGLFASP
DDVTLALRGMRTLDVRLERHYKNATTVAKWLETREEVEAVYYPALESHPQHQLWKRDFKG
ASGLLSFVTKPSTQAAADALLDSLSLFSIGYSWGGFESLAMIADPKPVRSATSWEIDGHL
VRLHIGLEDPSDLIEDLEQGFAKFNMLRG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory