SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO353_25185 FitnessBrowser__pseudo3_N2E3:AO353_25185 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q88H32 Ornithine cyclodeaminase; OCD; EC 4.3.1.12 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
62% identity, 95% coverage: 1:333/350 of query aligns to 1:334/350 of Q88H32

query
sites
Q88H32

M

M

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Y

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R45 (= R45) binding L-ornithine
K69 (= K69) binding L-ornithine
T84 (= T84) binding NAD(+)
R112 (= R112) binding L-ornithine; binding NAD(+)
AQ 139:140 (= AQ 139:140) binding NAD(+)
D161 (= D161) binding NAD(+)
T202 (= T202) binding NAD(+)
VGGD 225:228 (= VGGD 225:228) binding NAD(+)
D228 (= D228) binding L-ornithine
K232 (= K232) binding NAD(+)
S293 (= S293) binding NAD(+)
V294 (= V294) binding L-ornithine
K331 (= K330) binding NAD(+)

1x7dA Crystal structure analysis of ornithine cyclodeaminase complexed with NAD and ornithine to 1.6 angstroms (see paper)
62% identity, 95% coverage: 2:333/350 of query aligns to 1:333/340 of 1x7dA

query
sites
1x7dA

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active site: E55 (= E56), D227 (= D228)
binding nicotinamide-adenine-dinucleotide: T83 (= T84), R111 (= R112), T112 (= T113), G137 (= G138), A138 (= A139), Q139 (= Q140), D160 (= D161), T161 (≠ I162), V200 (= V201), T201 (= T202), A202 (= A203), I209 (= I210), V224 (= V225), G225 (= G226), D227 (= D228), K231 (= K232), S292 (= S293), V293 (= V294), G294 (= G295)
binding L-ornithine: R44 (= R45), V53 (= V54), E55 (= E56), M57 (= M58), K68 (= K69), V70 (= V71), N71 (= N72), G72 (= G73), R111 (= R112), D227 (= D228), V293 (= V294)

1u7hA Structure and a proposed mechanism for ornithine cyclodeaminase from pseudomonas putida (see paper)
62% identity, 95% coverage: 2:333/350 of query aligns to 1:333/341 of 1u7hA

query
sites
1u7hA

T

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active site: E55 (= E56), D227 (= D228)
binding nicotinamide-adenine-dinucleotide: T83 (= T84), R111 (= R112), T112 (= T113), G137 (= G138), A138 (= A139), Q139 (= Q140), D160 (= D161), T161 (≠ I162), V200 (= V201), T201 (= T202), A202 (= A203), I209 (= I210), V224 (= V225), G225 (= G226), D227 (= D228), K231 (= K232), S292 (= S293), V293 (= V294), G294 (= G295)

1omoA Alanine dehydrogenase dimer w/bound NAD (archaeal) (see paper)
32% identity, 80% coverage: 44:322/350 of query aligns to 40:319/320 of 1omoA

query
sites
1omoA

P

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C

G

P

P

G

G

K
|
K

T

Q

E

E

L

L

H

D

A

V

E

E

I

I

L

L

R

K

N

K

A

A

R

K

I

I

I

V

V

V

E

D

F

D

E

L

P

E

Q

Q

T

A

R

K

I

H

E

G

G

G

D

E

I

I

Q

N

Q

V

L

A

E

V

A

S

D

K

S

G

P

V

V

I

-

G

V

V

E

E

F

D

F

V

R

H

I

A

V

T

L

I

G

G

E

E

V

V

-

I

-

A

-

G

-

L

-

K

P

D

G

G

R

R

E

E

N

S

D

D

A

E

Q

E

V

I

T

T

V

I

F

F

D

D

S
|
S

V
x
T

G
|
G

F

L

A

A

L

I

E

Q

D

D

F

V

S

A

S

V

L

A

R

K

Y

V

L

V

H

Y

D

E

L

N

A

A

R

L

E

S

H

K

G

N

I

V

G

G

E

S

R

K

I

I

H

K

L

F

active site: R52 (≠ E56), D219 (= D228)
binding nicotinamide-adenine-dinucleotide: T109 (= T113), G134 (= G138), T135 (≠ A139), Q136 (= Q140), Y156 (≠ F160), D157 (= D161), V158 (≠ I162), R159 (≠ D163), T195 (≠ D204), P196 (≠ K205), G217 (= G226), D219 (= D228), K223 (= K232), S290 (= S293), T291 (≠ V294), G292 (= G295)

O28608 Alanine dehydrogenase; AlaDH; EC 1.4.1.1 from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see paper)
32% identity, 80% coverage: 44:322/350 of query aligns to 40:319/322 of O28608

query
sites
O28608

P

P

R

K

T

V

A

Y

N

L

H

E

S

F

A

E

D

K

G

G

V

D

I

L

E

R

L

A

M

M

P

P

T

A

D

H

D

L

G

M

Q

G

Q

Y

Y

A

S

G

F

L

K

K

Y

W

V

V

N

N

G

S

H

H

P

P

G

G

N

N

G

P

Q

D

R

K

D

G

L

L

L

P

T

T

V

V

M

M

A

A

F

L

G

M

V

I

L

L

A

N

D

S

V

P

H

E

S

T

G

G

Y

F

P

P

T

L

L

A

L

V

S

M

E

D

L

A

T

T

L

Y

T

T

A

S

V

L

R
|
R

T

T

A

G

A

A

T

A

S

G

A

G

L

I

V

A

A

A

Q

K

S

Y

L

L

A

A

R

R

P

K

G

N

A

S

S

S

V

V

M

F

A

G

I

F

I

I

G

G

N

C

G

G

A
x
T

Q
|
Q

S

A

E

Y

F

F

Q

Q

A

L

I

E

A

A

F

L

H

R

E

R

M

V

L

F

S

D

I

I

R

G

E

E

I

V

R

K

I

A

F

Y

D
|
D

I
x
V

D
x
R

R

E

D

K

A

A

S

A

L

K

K

K

L

F

K

V

H

S

N

Y

L

C

S

E

A

-

F

-

P

-

G

-

I

-

E

D

V

R

I

G

L

I

S

S

S

A

S

S

V

V

Q

Q

E

P

A

A

V

E

K

E

G

A

A

S

H

R

I

C

V

D

T

V

T

L

V

V

T

T

A

T

D

T

K

P

A

S

Y

R

A

K

T

P

I

V

L

V

T

K

P

A

E

E

M

W

I

V

E

E

P

E

G

G

M

T

H

H

I

I

N

N

A

A

V

I

G
|
G

G
x
A

D
|
D

C

G

P

P

G

G

K
|
K

T

Q

E

E

L

L

H

D

A

V

E

E

I

I

L

L

R

K

N

K

A

A

R

K

I

I

I

V

V

V

E

D

F

D

E

L

P

E

Q

Q

T

A

R

K

I

H

E

G

G

G

D

E

I

I

Q

N

Q

V

L

A

E

V

A

S

D

K

S

G

P

V

V

I

-

G

V

V

E

E

F

D

F

V

R

H

I

A

V

T

L

I

G

G

E

E

V

V

-

I

-

A

-

G

-

L

-

K

P

D

G

G

R

R

E

E

N

S

D

D

A

E

Q

E

V

I

T

T

V

I

F

F

D

D

S
|
S

V

T

G

G

F

L

A

A

L

I

E

Q

D

D

F

V

S

A

S

V

L

A

R

K

Y

V

L

V

H

Y

D

E

L

N

A

A

R

L

E

S

H

K

G

N

I

V

G

G

E

S

R

K

I

I

H

K

L

F

R108 (= R112) binding NAD(+)
TQ 135:136 (≠ AQ 139:140) binding NAD(+)
DVR 157:159 (≠ DID 161:163) binding NAD(+)
GAD 217:219 (≠ GGD 226:228) binding NAD(+)
K223 (= K232) binding NAD(+)
S290 (= S293) binding NAD(+)

6t3eB Structure of thermococcus litoralis delta(1)-pyrroline-2-carboxylate reductase in complex with nadh and l-proline (see paper)
31% identity, 77% coverage: 52:322/350 of query aligns to 48:325/325 of 6t3eB

query
sites
6t3eB

D

N

G

G

V

F

I

I

E

L

L

Y

M
|
M

P

P

T

S

-

Y

-

L

D

E

D

D

G

S

Q

E

Q

A

Y

L

S

A

F

V

K
|
K

Y

V

V

V

N

S

G

L

H

Y

P

P

G

E

N

N

G

T

Q

K

R

K

D

G

L

L

L

P

T
x
S

V

V

M

L

A

A

F

S

G

I

V

L

L

L

A

N

D

D

V

P

H

K

S

T

G

G

Y

A

P

P

T

L

L

A

L

L

S

M

E

E

L

G

T

T

L

F

T

I

T

T

A

A

V

M

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

G

L

V

V

A

A

T

Q

K

S

Y

L

L

A

A

R

R

P

K

G

D

A

S

S

K

V

I

M

A

A

G

I

I

G

G

N

A

G
|
G

A
x
V

Q
|
Q

S

A

E

R

F

T

Q

Q

A

L

I

W

A

A

F

V

H

C

E

E

M

V

L

R

S

N

I

I

R

E

E

K

I

A

R

L

I

V

F

Y

D
|
D

I
|
I

D

N

R

P

D

K

A

N

S

A

L

K

K

K

L

F

K

A

H

E

N

E

L

M

S

S

A

K

F

K

P

L

G

G

I

I

E

E

V

I

I

K

L

T

S

V

S

E

S

S

V

A

Q

R

E

E

A

A

V

T

K

E

G

K

A

S

H

D

I

I

V

L

T

-

V

I

T

V

A
|
A

D
x
T

K
x
T

A
|
A

Y

R

A

E

T

P

I
x
V

L

V

T

K

P

G

E

G

M

W

I

I

E

R

P

E

G

G

M

T

H

H

I

I

N

N

A

S

V
|
V

G
|
G

G
x
W

D

V

C

G

P

R

G

D

K

A

T

R

E

E

L

L

H

D

A

S

E

E

I

T

L

V

R

R

N

K

A

S

R

K

I

L

I

V

V

V

E

D

F

S

E

K

P

E

Q

G

T

V

R

L

I

N

E

E

-

S

G

G

D

D

I

I

Q

I

L

P

E

M

A

K

D

E

S

G

P

V

V

I

-

D

-

E

-

G

-

H

-

I

-

H

V

A

E

E

F

L

F

A

R

E

I

I

V

V

L

A

G

G

E

V

V

K

P

K

G

G

R

R

E

E

N

N

D

N

A

R

Q

E

V

I

T

T

V

L

F

F

D

K

S
|
S

V
|
V

G
|
G

F

L

A

A

L

I

E

E

D

D

F

A

S

I

S

T

L

A

R

K

Y

L

L

A

H

Y

D

E

L

K

A

A

R

L

E

E

H

H

G

G

I

V

G

G

E

T

R

N

I

V

H

E

L

L

binding 1,4-dihydronicotinamide adenine dinucleotide: S82 (≠ T84), T111 (= T113), G136 (= G138), V137 (≠ A139), Q138 (= Q140), D159 (= D161), I160 (= I162), A199 (= A203), T200 (≠ D204), T201 (≠ K205), A202 (= A206), V206 (≠ I210), V221 (= V225), G222 (= G226), W223 (≠ G227), S296 (= S293), V297 (= V294), G298 (= G295)
binding proline: M54 (= M58), K67 (= K69), R110 (= R112)

Sites not aligning to the query:

binding proline: 39

5gzlA Cyclodeaminase_pa
31% identity, 81% coverage: 53:335/350 of query aligns to 64:347/357 of 5gzlA

query
sites
5gzlA

G

G

V
x
I

I

W

E
|
E

L

W

M

M

P

P

-

H

T

R

D

E

D

P

G

G

Q

D

Q

H

Y

I

S

T

F

L

K

K

Y

T

V

V

N

G

G
x
Y

H

S

P

P

G

A

N

N

G

P

Q

A

R

R

-

F

D

G

L

L

L

P

T
|
T

V
x
I

M

L

A

G

F

T

G

V

V

A

L

R

A

Y

D

D

V

D

H

T

S

T

G

G

Y

A

P

L

T

T

L

A

L

L

S

M

E

D

L

G

T

V

L

L

T

L

T

T

A

A

V

L

R

R

T
|
T

A

G

A

A

T

A

S

S

A

A

L

V

V

A

A

S

Q

R

S

L

L

L

A

A

R

R

P

P

G

D

A

S

S

H

V

T

M

L

A

G

I

L

I

I

G

G

N

T

G
|
G

A
|
A

Q
|
Q

S

A

E

V

F

T

Q

Q

A

L

I

H

A

A

F

L

H

S

E

L

M

V

L

L

S

P

I

L

R

Q

E

R

I

A

R

L

I

V

F

W

D
|
D

I
x
T

D

D

R

-

D

P

A

A

S
x
H

L

R

K

E

L

S

K

F

H

A

N

R

L

R

S

A

A

A

F

F

P

T

G

G

I

V

E

S

V

V

I

E

L

I

S

A

S

E

S

P

V

A

Q

R

E

I

A

A

V

A

K

E

G

A

A

D

H

V

I

I

V

S

T

T

T
x
A

V
x
T

T
x
S

A

V

D

A

K

V

A

G

Y

Q

A

G

T

P

I
x
V

L

L

T

P

P

D

E

T

M

G

I

V

E

R

P

E

G

H

M

L

H

H

I

I

N

N

A

A

V
|
V

G
|
G

G
x
A

D
|
D

C

L

P

V

G

G

K
|
K

T

T

E

E

L

L

H

P

A

L

E

G

I

L

L

L

R

E

N

R

A

A

R

F

I

V

I

T

V

A

E

D

F

H

E

P

P

E

Q

Q

T

A

R

L

I
x
R

E
|
E

G

G

D

E

I

C

Q

Q

L

L

E

S

A

A

D

D

-

R

-

L

S

G

P

P

V

Q

V

L

E

A

F

H

F

L

R

C

I

A

V

D

L

P

G

A

E

A

V

A

P

A

G

G

R

R

E

Q

N

D

D

-

A

-

Q

T

V

L

T

S

V

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

L

F

E

E

D

D

F

A

S

L

S

A

L

M

R

E

Y

V

L

F

H

L

D

E

L

A

A

A

R

A

E

E

H

R

G

D

I

L

G

G

E

I

R

R

I

V

H

G

L

I

V

E

P

H

T

H

P

P

A

G

N

D

I

A

K

L

D

D

L

P

Y

Y

Q

A

L

L

binding lysine: I65 (≠ V54), E67 (= E56), D240 (= D228), R267 (≠ I255), E268 (= E256)
binding nicotinamide-adenine-dinucleotide: Y85 (≠ G73), T97 (= T84), I98 (≠ V85), T126 (= T113), G151 (= G138), A152 (= A139), Q153 (= Q140), D174 (= D161), T175 (≠ I162), H179 (≠ S167), A212 (≠ T200), T213 (≠ V201), S214 (≠ T202), V222 (≠ I210), V237 (= V225), G238 (= G226), A239 (≠ G227), D240 (= D228), K244 (= K232), S305 (= S293), T306 (≠ V294), G307 (= G295)

Sites not aligning to the query:

binding lysine: 55, 57

5gziA Cyclodeaminase_pa
31% identity, 81% coverage: 53:335/350 of query aligns to 64:347/354 of 5gziA

query
sites
5gziA

G

G

V

I

I

W

E

E

L

W

M

M

P

P

-

H

T

R

D

E

D

P

G

G

Q

D

Q

H

Y

I

S

T

F

L

K
|
K

Y

T

V

V

N

G

G
x
Y

H

S

P

P

G

A

N

N

G

P

Q

A

R

R

-

F

D

G

L

L

L

P

T
|
T

V

I

M

L

A

G

F

T

G

V

V

A

L

R

A

Y

D

D

V

D

H

T

S

T

G

G

Y

A

P

L

T

T

L

A

L

L

S

M

E

D

L

G

T

V

L

L

T

L

T

T

A

A

V

L

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

A

L

V

V

A

A

S

Q

R

S

L

L

L

A

A

R

R

P

P

G

D

A

S

S

H

V

T

M

L

A

G

I

L

I

I

G

G

N

T

G
|
G

A
|
A

Q
|
Q

S

A

E

V

F

T

Q

Q

A

L

I

H

A

A

F

L

H

S

E

L

M

V

L

L

S

P

I

L

R

Q

E

R

I

A

R

L

I

V

F

W

D
|
D

I
x
T

D

D

R

-

D

P

A

A

S
x
H

L

R

K

E

L

S

K

F

H

A

N

R

L

R

S

A

A

A

F

F

P

T

G

G

I

V

E

S

V

V

I

E

L

I

S

A

S

E

S

P

V

A

Q

R

E

I

A

A

V

A

K

E

G

A

A

D

H

V

I

I

V

S

T

T

T
x
A

V
x
T

T
x
S

A
x
V

D

A

K

V

A

G

Y

Q

A

G

T

P

I

V

L

L

T

P

P

D

E

T

M

G

I

V

E

R

P

E

G

H

M

L

H

H

I

I

N

N

A

A

V
|
V

G
|
G

G
x
A

D

D

C

L

P

V

G

G

K

K

T

T

E

E

L

L

H

P

A

L

E

G

I

L

L

L

R

E

N

R

A

A

R

F

I

V

I

T

V

A

E

D

F

H

E

P

P

E

Q

Q

T

A

R

L

I

R

E

E

G

G

D

E

I

C

Q

Q

L

L

E

S

A

A

D

D

-

R

-

L

S

G

P

P

V

Q

V

L

E

A

F

H

F

L

R

C

I

A

V

D

L

P

G

A

E

A

V

A

P

A

G

G

R

R

E

Q

N

D

D

-

A

-

Q

T

V

L

T

S

V

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

L

F

E

E

D

D

F

A

S

L

S

A

L

M

R

E

Y

V

L

F

H

L

D

E

L

A

A

A

R

A

E

E

H

R

G

D

I

L

G

G

E

I

R

R

I

V

H

G

L

I

V

E

P

H

T

H

P

P

A

G

N

D

I

A

K

L

D

D

L

P

Y

Y

Q

A

L

L

binding nicotinamide-adenine-dinucleotide: Y85 (≠ G73), T97 (= T84), R125 (= R112), T126 (= T113), G151 (= G138), A152 (= A139), Q153 (= Q140), D174 (= D161), T175 (≠ I162), H179 (≠ S167), A212 (≠ T200), T213 (≠ V201), S214 (≠ T202), V215 (≠ A203), V237 (= V225), G238 (= G226), A239 (≠ G227), S305 (= S293), T306 (≠ V294), G307 (= G295)
binding (2S)-piperidine-2-carboxylic acid: K81 (= K69), R125 (= R112), A239 (≠ G227), T306 (≠ V294), G307 (= G295)

Sites not aligning to the query:

binding (2S)-piperidine-2-carboxylic acid: 53

5yu4A Structural basis for recognition of l-lysine, l-ornithine, and l-2,4- diamino butyric acid by lysine cyclodeaminase (see paper)
31% identity, 81% coverage: 53:335/350 of query aligns to 60:343/344 of 5yu4A

query
sites
5yu4A

G

G

V

I

I

W

E
|
E

L

W

M

M

P

P

-

H

T

R

D

E

D

P

G

G

Q

D

Q

H

Y

I

S

T

F

L

K
|
K

Y

T

V

V

N

G

G
x
Y

H

S

P

P

G

A

N

N

G

P

Q

A

R

R

-

F

D

G

L

L

L

P

T
|
T

V
x
I

M

L

A

G

F

T

G

V

V

A

L

R

A

Y

D

D

V

D

H

T

S

T

G

G

Y

A

P

L

T

T

L

A

L

L

S

M

E

D

L

G

T

V

L

L

T

L

T

T

A

A

V

L

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

A

L

V

V

A

A

S

Q

R

S

L

L

L

A

A

R

R

P

P

G

D

A

S

S

H

V

T

M

L

A

G

I

L

I

I

G

G

N

T

G
|
G

A
|
A

Q
|
Q

S

A

E

V

F

T

Q

Q

A

L

I

H

A

A

F

L

H

S

E

L

M

V

L

L

S

P

I

L

R

Q

E

R

I

A

R

L

I

V

F

W

D
|
D

I
x
T

D

D

R

-

D

P

A

A

S
x
H

L

R

K

E

L

S

K

F

H

A

N

R

L

R

S

A

A

A

F

F

P

T

G

G

I

V

E

S

V

V

I

E

L

I

S

A

S

E

S

P

V

A

Q

R

E

I

A

A

V

A

K

E

G

A

A

D

H

V

I

I

V

S

T

T

T
x
A

V
x
T

T
x
S

A
x
V

D

A

K

V

A

G

Y

Q

A

G

T

P

I
x
V

L

L

T

P

P

D

E

T

M

G

I

V

E

R

P

E

G

H

M

L

H

H

I

I

N

N

A

A

V
|
V

G

G

G
x
A

D

D

C

L

P

V

G

G

K

K

T

T

E

E

L

L

H

P

A

L

E

G

I

L

L

L

R

E

N

R

A

A

R

F

I

V

I

T

V

A

E

D

F

H

E

P

P

E

Q

Q

T

A

R

L

I

R

E

E

G

G

D

E

I

C

Q

Q

L

L

E

S

A

A

D

D

-

R

-

L

S

G

P

P

V

Q

V

L

E

A

F

H

F

L

R

C

I

A

V

D

L

P

G

A

E

A

V

A

P

A

G

G

R

R

E

Q

N

D

D

-

A

-

Q

T

V

L

T

S

V

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

L

F

E

E

D

D

F

A

S

L

S

A

L

M

R

E

Y

V

L

F

H

L

D

E

L

A

A

A

R

A

E

E

H

R

G

D

I

L

G

G

E

I

R

R

I

V

H

G

L

I

V

E

P

H

T

H

P

P

A

G

N

D

I

A

K

L

D

D

L

P

Y

Y

Q

A

L

L

binding 2,4-diaminobutyric acid: E63 (= E56), K77 (= K69), R121 (= R112), T302 (≠ V294), G303 (= G295)
binding nicotinamide-adenine-dinucleotide: Y81 (≠ G73), T93 (= T84), I94 (≠ V85), R121 (= R112), T122 (= T113), G147 (= G138), A148 (= A139), Q149 (= Q140), D170 (= D161), T171 (≠ I162), H175 (≠ S167), A208 (≠ T200), T209 (≠ V201), S210 (≠ T202), V211 (≠ A203), V218 (≠ I210), V233 (= V225), A235 (≠ G227), S301 (= S293), T302 (≠ V294), G303 (= G295)

5yu3A Structural basis for recognition of l-lysine, l-ornithine, and l-2,4- diamino butyric acid by lysine cyclodeaminase (see paper)
31% identity, 81% coverage: 53:335/350 of query aligns to 60:343/344 of 5yu3A

query
sites
5yu3A

G

G

V

I

I

W

E

E

L

W

M
|
M

P

P

-

H

T

R

D

E

D

P

G

G

Q

D

Q

H

Y

I

S

T

F

L

K
|
K

Y

T

V

V

N

G

G
x
Y

H

S

P

P

G

A

N

N

G

P

Q

A

R

R

-

F

D

G

L

L

L

P

T
|
T

V
x
I

M

L

A

G

F

T

G

V

V

A

L

R

A

Y

D

D

V

D

H

T

S

T

G

G

Y

A

P

L

T

T

L

A

L

L

S

M

E

D

L

G

T

V

L

L

T

L

T

T

A

A

V

L

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

A

L

V

V

A

A

S

Q

R

S

L

L

L

A

A

R

R

P

P

G

D

A

S

S

H

V

T

M

L

A

G

I

L

I

I

G

G

N

T

G
|
G

A
|
A

Q
|
Q

S

A

E

V

F

T

Q

Q

A

L

I

H

A

A

F

L

H

S

E

L

M

V

L

L

S

P

I

L

R

Q

E

R

I

A

R

L

I

V

F

W

D
|
D

I
x
T

D

D

R

-

D

P

A

A

S

H

L

R

K

E

L

S

K

F

H

A

N

R

L

R

S

A

A

A

F

F

P

T

G

G

I

V

E

S

V

V

I

E

L

I

S

A

S

E

S

P

V

A

Q

R

E

I

A

A

V

A

K

E

G

A

A

D

H

V

I

I

V

S

T

T

T
x
A

V
x
T

T
x
S

A
x
V

D

A

K

V

A

G

Y

Q

A

G

T

P

I

V

L

L

T

P

P

D

E

T

M

G

I

V

E

R

P

E

G

H

M

L

H

H

I

I

N

N

A

A

V
|
V

G

G

G
x
A

D

D

C

L

P

V

G

G

K

K

T

T

E

E

L

L

H

P

A

L

E

G

I

L

L

L

R

E

N

R

A

A

R

F

I

V

I

T

V

A

E

D

F

H

E

P

P

E

Q

Q

T

A

R

L

I

R

E

E

G

G

D

E

I

C

Q

Q

L

L

E

S

A

A

D

D

-

R

-

L

S

G

P

P

V

Q

V

L

E

A

F

H

F

L

R

C

I

A

V

D

L

P

G

A

E

A

V

A

P

A

G

G

R

R

E

Q

N

D

D

-

A

-

Q

T

V

L

T

S

V

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

L

F

E

E

D

D

F

A

S

L

S

A

L

M

R

E

Y

V

L

F

H

L

D

E

L

A

A

A

R

A

E

E

H

R

G

D

I

L

G

G

E

I

R

R

I

V

H

G

L

I

V

E

P

H

T

H

P

P

A

G

N

D

I

A

K

L

D

D

L

P

Y

Y

Q

A

L

L

binding nicotinamide-adenine-dinucleotide: Y81 (≠ G73), T93 (= T84), I94 (≠ V85), T122 (= T113), G147 (= G138), A148 (= A139), Q149 (= Q140), D170 (= D161), T171 (≠ I162), A208 (≠ T200), T209 (≠ V201), S210 (≠ T202), V211 (≠ A203), V233 (= V225), A235 (≠ G227), S301 (= S293), T302 (≠ V294), G303 (= G295)
binding proline: M65 (= M58), K77 (= K69), R121 (= R112)

4bv9A Crystal structure of the NADPH form of mouse mu-crystallin. (see paper)
34% identity, 63% coverage: 90:309/350 of query aligns to 88:299/303 of 4bv9A

query
sites
4bv9A

V

L

L

L

A

F

D

D

V

P

H

S

S

N

G

G

Y

S

P

L

T

L

L

A

L

V

S

M

E

D

L

G

T

N

L

V

T

I

T

T

A

A

V

K

R
|
R

T
|
T

A

A

T

V

S

S

A

A

L

I

V

A

A

T

Q

K

S

L

L

L

A

K

R

P

P

P

G

G

A

S

S

D

V

V

M

L

A

C

I

I

I

L

G
|
G

N

A

G
|
G

A
x
V

Q
|
Q

S

A

E

Y

F

S

Q

H

A

Y

I

E

A

I

F

F

H

T

E

E

M

Q

L

F

S

S

I

F

R

K

E

E

I

V

R

R

I

M

F

W

D
x
N

I
x
R

D
x
T

R

R

D

E

A

N

S

A

L

E

K

K

L

F

K

A

H

S

N

T

L

V

S

Q

A

G

F

-

P

-

G

-

I

-

E

D

V

V

I

R

L

V

S

C

S

S

V

V

Q

Q

E

E

A

A

V

V

K

T

G

G

A

A

H

D

I

V

V

I

T

I

T

T

V
|
V

T
|
T

A

-

D

-

K
x
M

A
|
A

Y

T

A

E

T

P

I

I

L

L

T

F

P

G

E

E

M

W

I

V

E

K

P

P

G

G

M

A

H

H

I

I

N

N

A

A

V
|
V

G
|
G

G

A

D
x
S

C

R

P

P

G

D

K

W

T

R

E

E

L

L

H

D

A

D

E

E

I

L

L

M

R

R

N

Q

A

A

R

V

I

L

I

Y

V

V

E

D

F

S

-

R

E

E

P

A

Q

A

T

L

R

K

I

E

E

S

G

G

D

D

I

V

Q

L

L

S

E

G

A

A

D

D

S

I

P

-

V

F

V

A

E

E

F

L

F

G

R

E

I

V

V

I

L

S

G

G

E

A

V

K

P

P

G

A

R

H

E

C

N

E

D

-

A

-

Q

K

V

T

T

T

V

V

F

F

D

K

S
|
S

V
x
L

G
|
G

F

M

A

A

L

V

E

E

D

D

F

L

S

V

S

A

L

A

R

K

Y

L

L

V

H

Y

D

D

active site: S220 (≠ D228)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T111 (= T113), G134 (= G136), G136 (= G138), V137 (≠ A139), Q138 (= Q140), N159 (≠ D161), R160 (≠ I162), T161 (≠ D163), V195 (= V201), T196 (= T202), M197 (≠ K205), A198 (= A206), V217 (= V225), G218 (= G226), S283 (= S293), L284 (≠ V294), G285 (= G295)
binding pyruvic acid: R110 (= R112)

Sites not aligning to the query:

active site: 58
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 82, 83
binding pyruvic acid: 45, 60, 73

4bvaA Crystal structure of the NADPH-t3 form of mouse mu-crystallin. (see paper)
34% identity, 63% coverage: 90:309/350 of query aligns to 87:298/303 of 4bvaA

query
sites
4bvaA

V

L

L

L

A

F

D

D

V

P

H

S

S

N

G

G

Y

S

P

L

T

L

L

A

L

V

S

M

E

D

L

G

T

N

L

V

T

I

T
|
T

A

A

V

K

R
|
R

T
|
T

A

A

T

V

S

S

A

A

L

I

V

A

A

T

Q

K

S

L

L

L

A

K

R

P

P

P

G

G

A

S

S

D

V

V

M

L

A

C

I

I

I

L

G

G

N

A

G
|
G

A
x
V

Q
|
Q

S

A

E

Y

F

S

Q

H

A

Y

I

E

A

I

F

F

H

T

E

E

M

Q

L

F

S

S

I

F

R

K

E

E

I

V

R

R

I

M

F

W

D
x
N

I
x
R

D
x
T

R

R

D

E

A
x
N

S

A

L

E

K

K

L

F

K

A

H

S

N

T

L

V

S

Q

A

G

F

-

P

-

G

-

I

-

E

D

V

V

I

R

L

V

S

C

S

S

V

V

Q

Q

E

E

A

A

V

V

K

T

G

G

A

A

H

D

I

V

V

I

T

I

T

T

V
|
V

T
|
T

A

-

D

-

K
x
M

A
|
A

Y

T

A

E

T

P

I

I

L

L

T

F

P

G

E

E

M

W

I

V

E

K

P

P

G

G

M

A

H

H

I

I

N

N

A

A

V
|
V

G

G

G

A

D
x
S

C
x
R

P

P

G

D

K
x
W

T

R

E

E

L

L

H

D

A

D

E

E

I

L

L

M

R

R

N

Q

A

A

R

V

I

L

I

Y

V

V

E

D

F

S

-

R

E

E

P

A

Q

A

T

L

R

K

I
x
E

E

S

G

G

D

D

I

V

Q

L

L

S

E

G

A

A

D

D

S

I

P

-

V

F

V

A

E

E

F

L

F

G

R

E

I

V

V

I

L

S

G

G

E

A

V

K

P

P

G

A

R

H

E

C

N

E

D

-

A

-

Q

K

V

T

T

T

V

V

F

F

D

K

S
|
S

V
x
L

G
|
G

F

M

A

A

L

V

E

E

D

D

F

L

S

V

S

A

L

A

R

K

Y

L

L

V

H

Y

D

D

active site: S219 (≠ D228)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T106 (= T109), R109 (= R112), T110 (= T113), G135 (= G138), V136 (≠ A139), Q137 (= Q140), N158 (≠ D161), R159 (≠ I162), T160 (≠ D163), N163 (≠ A166), V194 (= V201), T195 (= T202), M196 (≠ K205), A197 (= A206), V216 (= V225), S282 (= S293), L283 (≠ V294), G284 (= G295)
binding 3,5,3'triiodothyronine: S219 (≠ D228), R220 (≠ C229), W223 (≠ K232), E247 (≠ I255)

Sites not aligning to the query:

active site: 59
binding 3,5,3'triiodothyronine: 46, 57, 59, 76, 78

Q14894 Ketimine reductase mu-crystallin; NADP-regulated thyroid-hormone-binding protein; EC 1.5.1.25 from Homo sapiens (Human) (see paper)
34% identity, 60% coverage: 100:309/350 of query aligns to 105:308/314 of Q14894

query
sites
Q14894

T

T

L

L

S

A

E

V

L

M

-

D

-

G

T

N

L

V

T

I

T

T

A

A

V

K

R

R

T

T

A

A

T

V

S

S

A

A

L

I

V

A

A

T

Q

K

S

F

L

L

A

K

R

P

P

P

G

S

A

S

S

E

V

V

M

L

A

C

I

I

I

L

G
|
G

N
x
A

G
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G

A
x
V

Q
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Q

S
x
A

E

Y

F

S

Q

H

A

Y

I

E

A

I

F

F

H

T

E

E

M

Q

L

F

S

S

I

F

R

K

E

E

I

V

R

R

I

I

F

W

D
x
N

I
x
R

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K

D

E

A

N

S

A

L

E

K

K

L

F

K

A

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A

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-

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-

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C

S

S

V

V

Q

Q

E

E

A

A

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V

K

A

G

G

A

A

H

D

I

V

V

I

T

I

T

T

V

V

T

T

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D

-

K

-

A

A

Y

T

A

E

T

P

I

I

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F

P

G

E

E

M

W

I

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E

K

P

P

G

G

M

A

H

H

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I

N

N

A

A

V

V

G

G

G

A

D

S

C

R

P

P

G

D

K

W

T

R

E

E

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D

A

D

E

E

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N

E

A

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V

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E

D

F

S

-

Q

E

E

P

A

Q

A

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I

E

E

S

G

G

D

D

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-

Q

L

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D

A

S

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P

I

V

F

V

A

E

E

F

L

F

G

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E

I

V

V

I

L

K

G

G

E

V

V

K

P

P

G

A

R

-

E

-

N

H

D

C

A

E

Q

K

V

T

T

T

V

V

F

F

D

K

S

S

V

L

G

G

F

M

A

A

L

V

E

E

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D

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S

A

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A

R

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Y

L

L

I

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Y

D

D

GAGVQA 143:148 (≠ GNGAQS 136:141) binding NADP(+)
N168 (≠ D161) binding NADP(+)
R169 (≠ I162) binding NADP(+)

2i99A Crystal structure of human mu_crystallin at 2.6 angstrom (see paper)
33% identity, 60% coverage: 100:309/350 of query aligns to 104:307/312 of 2i99A

query
sites
2i99A

T

T

L

L

S

A

E

V

L

M

-

D

-

G

T

N

L

V

T

I

T

T

A

A

V

K

R
|
R

T
|
T

A

A

T

V

S

S

A

A

L

I

V

A

A

T

Q

K

S

F

L

L

A

K

R

P

P

P

G

S

A

S

S

E

V

V

M

L

A

C

I

I

I

L

G
|
G

N
x
A

G
|
G

A
x
V

Q
|
Q

S

A

E

Y

F

S

Q

H

A

Y

I

E

A

I

F

F

H

T

E

E

M

Q

L

F

S

S

I

F

R

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E

E

I

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R

R

I

I

F

W

D
x
N

I
x
R

D
x
T

R

K

D

E

A

N

S

A

L

E

K

K

L

F

K

A

H

D

N

T

L

V

S

Q

A

G

F

-

P

-

G

-

I

-

E

E

V

V

I

R

L

V

S

C

S

S

V

V

Q

Q

E

E

A

A

V

V

K

A

G

G

A

A

H

D

I

V

V

I

T

I

T

T

V
|
V

T
|
T

A
x
L

D

-

K

-

A
|
A

Y

T

A

E

T

P

I

I

L

L

T

F

P

G

E

E

M

W

I

V

E

K

P

P

G

G

M

A

H

H

I

I

N

N

A

A

V
|
V

G
|
G

G

A

D
x
S

C

R

P

P

G

D

K

W

T

R

E

E

L

L

H

D

A

D

E

E

I

L

L

M

R

K

N

E

A

A

R

V

I

L

I

Y

V

V

E

D

F

S

E

Q

P

E

Q

A

T

A

R

L

I

K

E

E

G

S

D

G

I

D

Q

V

Q

L

L

L

E

S

A

G

D

A

S

E

P

I

V

F

V

A

E

E

F

L

F

G

R

E

I

V

V

I

L

K

G

G

E

V

V

K

P

P

G

A

R

-

E

-

N

H

D

C

A

E

Q

K

V

T

T

T

V

V

F

F

D

K

S
|
S

V
x
L

G
|
G

F

M

A

A

L

V

E

E

D

D

F

T

S

V

S

A

L

A

R

K

Y

L

L

I

H

Y

D

D

active site: S228 (≠ D228)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R118 (= R112), T119 (= T113), G142 (= G136), A143 (≠ N137), G144 (= G138), V145 (≠ A139), Q146 (= Q140), N167 (≠ D161), R168 (≠ I162), T169 (≠ D163), V203 (= V201), T204 (= T202), L205 (≠ A203), A206 (= A206), V225 (= V225), G226 (= G226), S291 (= S293), L292 (≠ V294), G293 (= G295)

Sites not aligning to the query:

active site: 59
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 81, 90, 91

4m54A The structure of the staphyloferrin b precursor biosynthetic enzyme sbnb bound to n-(1-amino-1-carboxyl-2-ethyl)-glutamic acid and nadh (see paper)
25% identity, 80% coverage: 44:322/350 of query aligns to 44:309/310 of 4m54A

query
sites
4m54A

P

P

R
x
Y

T

L

A

R

N

Q

H

H

S

I

A

A

D

D

G
x
R

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I
|
I

E

A

L

-

M
|
M

P

P

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S

D

H

D

I

G

G

Q

G

Q

E

Y

H

S

A

F

I

-

S

-

G

-

I

K
|
K

Y

W

V
x
I

N

G

G

S

-

K

H

H

P

D

G

N

N

P

G

S

Q

K

R

R

D

N

L

M

L

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T
x
R

V

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A

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G

I

V

I

L

L

A

N

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Y

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R
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R

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A

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A

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I

V

A

A

A

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S

H

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L

A

A

R

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P

K

G

G

A

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M

L

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G

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x
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D

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x
Q

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E

A

A

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C

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A

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A

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|
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x
T

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D

K

Q

A

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Y

A

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I

-

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I

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E

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M

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I

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A

N

V
x
I

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G

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x
M

E

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H

A

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I

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A

A

-

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R

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I

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F

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Q

Q

T

C

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N

I

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Q

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E

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A

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-

I

-

V

V

L

T

G

G

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P

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G

R

R

E

E

N

D

D

D

A

D

Q

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F

L

D

N