SitesBLAST

P00099 Cytochrome c-551; Cytochrome C8; Cytochrome c551 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
48% identity, 38% coverage: 132:212/212 of query aligns to 24:103/104 of P00099

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H38 (= H146) binding axial binding residue
M83 (= M191) binding axial binding residue

Sites not aligning to the query:

1:22 signal peptide
23:104 modified: mature protein, Cytochrome c-551

351cA Structure of cytochrome c551 from p. Aeruginosa refined at 1.6 angstroms resolution and comparison of the two redox forms (see paper)
48% identity, 38% coverage: 132:212/212 of query aligns to 2:81/82 of 351cA

query
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351cA

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binding protoporphyrin ix containing fe: C12 (= C142), C15 (= C145), H16 (= H146), G24 (= G154), P25 (= P155), Y27 (= Y157), L44 (≠ V174), R47 (≠ S177), I48 (= I178), Q53 (≠ T183), G54 (= G184), V55 (≠ K185), W56 (= W186), G57 (= G187), I59 (= I189), P60 (= P190), M61 (= M191)

2exvA Crystal structure of the f7a mutant of the cytochrome c551 from pseudomonas aeruginosa (see paper)
48% identity, 38% coverage: 132:212/212 of query aligns to 2:81/82 of 2exvA

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2exvA

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binding heme c: C12 (= C142), C15 (= C145), H16 (= H146), G24 (= G154), P25 (= P155), R47 (≠ S177), I48 (= I178), S52 (≠ G182), Q53 (≠ T183), G54 (= G184), V55 (≠ K185), W56 (= W186), G57 (= G187), I59 (= I189), P60 (= P190), M61 (= M191), N64 (≠ T195)

2d0sA Crystal structure of the cytochrome c552 from moderate thermophilic bacterium, hydrogenophilus thermoluteolus (see paper)
41% identity, 36% coverage: 135:210/212 of query aligns to 3:76/79 of 2d0sA

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2d0sA

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binding heme c: C10 (= C142), C13 (= C145), H14 (= H146), P23 (= P155), Y32 (= Y164), K43 (≠ S177), V44 (≠ I178), A49 (≠ T183), G50 (= G184), V51 (≠ K185), W52 (= W186), G53 (= G187), V55 (≠ I189), M57 (= M191), P58 (= P192), H60 (≠ F194)

1a56A Primary sequence and solution conformation of ferricytochromE C-552 from nitrosomonas europaea, nmr, mean structure refined with explicit hydrogen bond constraints (see paper)
44% identity, 35% coverage: 136:210/212 of query aligns to 4:78/81 of 1a56A

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1a56A

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binding heme c: N9 (≠ S141), C10 (= C142), C13 (= C145), H14 (= H146), V21 (= V153), G22 (= G154), P23 (= P155), K45 (≠ S177), I46 (= I178), S50 (≠ G182), W54 (= W186), Q56 (≠ N188), I57 (= I189), M59 (= M191), N62 (≠ F194), V63 (≠ T195), N64 (≠ Q196)

5xecC Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
47% identity, 36% coverage: 136:212/212 of query aligns to 4:79/80 of 5xecC

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binding heme c: C10 (= C142), C13 (= C145), H14 (= H146), P23 (= P155), Y25 (= Y157), L42 (≠ V174), R45 (≠ S177), Q51 (≠ T183), V53 (≠ K185), W54 (= W186), G55 (= G187), I57 (= I189), M59 (= M191), N62 (≠ T195)

5xecA Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
42% identity, 37% coverage: 132:210/212 of query aligns to 2:79/82 of 5xecA

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binding heme c: C12 (= C142), C15 (= C145), H16 (= H146), G24 (= G154), P25 (= P155), Y27 (= Y157), Y34 (= Y164), Y43 (≠ K173), I48 (= I178), S53 (≠ T183), G54 (= G184), V55 (≠ K185), W56 (= W186), G57 (= G187), V59 (≠ I189), M61 (= M191), Q64 (≠ T195), I78 (≠ V209)

1corA Investigation of the solution conformation of cytochromE C-551 from pseudomonas stutzeri (see paper)
42% identity, 38% coverage: 132:212/212 of query aligns to 2:81/82 of 1corA

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1corA

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binding heme c: K10 (≠ N140), C12 (= C142), C15 (= C145), H16 (= H146), V23 (= V153), G24 (= G154), P25 (= P155), L44 (≠ V174), H47 (≠ S177), I48 (= I178), S52 (≠ G182), Q53 (≠ T183), G54 (= G184), V55 (≠ K185), W56 (= W186), G57 (= G187), I59 (= I189), M61 (= M191), N64 (≠ T195)

6kq1A Crystal structure of cytochrome c551 from pseudomonas sp. Strain mt-1.
44% identity, 37% coverage: 132:210/212 of query aligns to 2:79/82 of 6kq1A

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binding heme c: C12 (= C142), C15 (= C145), H16 (= H146), G24 (= G154), P25 (= P155), V30 (= V160), Y34 (= Y164), L44 (≠ V174), H47 (≠ S177), I48 (= I178), Q53 (≠ T183), G54 (= G184), N55 (≠ K185), W56 (= W186), G57 (= G187), I59 (= I189), M61 (= M191), N64 (≠ T195)
binding zinc ion: D2 (= D132), E4 (≠ M134), K10 (≠ N140), E70 (≠ D201)

5aurA Hydrogenobacter thermophilus cytochrome c552 dimer formed by domain swapping at n-terminal region (see paper)
40% identity, 35% coverage: 136:210/212 of query aligns to 4:80/83 of 5aurA

query
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binding heme c: C10 (= C142), C13 (= C145), H14 (= H146), P26 (= P155), Y28 (= Y157), Y35 (= Y164), Y44 (≠ K173), I49 (= I178), S54 (≠ T183), G55 (= G184), V56 (≠ K185), W57 (= W186), G58 (= G187), V60 (≠ I189), P61 (= P190), M62 (= M191), Q65 (≠ T195)

1cchA The solution conformation of cytochromE C-551 from p.Stutzeri zobell determined by nmr+ (see paper)
42% identity, 37% coverage: 132:210/212 of query aligns to 2:79/82 of 1cchA

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binding heme c: C12 (= C142), C15 (= C145), H16 (= H146), L44 (≠ V174), H47 (≠ S177), I48 (= I178), S52 (≠ G182), Q53 (≠ T183), V55 (≠ K185), W56 (= W186), G57 (= G187), I59 (= I189), M61 (= M191), P62 (= P192), N64 (≠ T195)

Query Sequence

>AO353_25240 FitnessBrowser__pseudo3_N2E3:AO353_25240
MKRMTTLLCAVQVCLATMANAAPLSITLPPETAVLKPNALPGYPLAQQKCSTCHSADYIN
FQPPGMSLAQWTGEASKMQHVYGAPISDQDVSIIGAYLAVTYGSAHASDADVQAASNPSA
GQTPAAPLAKVDAMALLQSNSCLSCHATDHKVVGPAYHDVAAKYANDPQALAKVTSSIQQ
GGTGKWGNIPMPPFTQLSPDDLHTLATFVLRQ

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory