SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO353_25420 AO353_25420 spermidine/putrescine ABC transporter ATP-binding protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
49% identity, 88% coverage: 12:340/372 of query aligns to 18:341/378 of P69874

query
sites
P69874

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C26 (≠ V20) mutation to A: Lower ATPase activity and transport efficiency.
F27 (≠ Y21) mutation to L: Lower ATPase activity and transport efficiency.
F45 (= F43) mutation to L: Lower ATPase activity and transport efficiency.
C54 (= C52) mutation to T: Loss of ATPase activity and transport.
L60 (= L58) mutation to F: Lower ATPase activity and transport efficiency.
L76 (= L74) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V137) mutation to M: Loss of ATPase activity and transport.
D172 (= D174) mutation to N: Loss of ATPase activity and transport.
C276 (≠ L278) mutation to A: Lower ATPase activity and transport efficiency.
E297 (= E299) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
47% identity, 78% coverage: 12:302/372 of query aligns to 7:298/375 of 2d62A

query
sites
2d62A

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binding pyrophosphate 2-: P40 (= P49), S41 (= S50), G42 (= G51), C43 (= C52), G44 (= G53), K45 (= K54), T46 (= T55), T47 (= T56)

1g291 Malk (see paper)
46% identity, 78% coverage: 11:302/372 of query aligns to 3:295/372 of 1g291

query
sites
1g291

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binding magnesium ion: D69 (= D81), E71 (vs. gap), K72 (vs. gap), K79 (≠ Y85), D80 (≠ Q86), Y228 (= Y238), D229 (≠ E239), E292 (= E299), D293 (≠ R300)
binding pyrophosphate 2-: P37 (= P49), S38 (= S50), G39 (= G51), C40 (= C52), G41 (= G53), K42 (= K54), T43 (= T55), T44 (= T56)

Sites not aligning to the query:

binding magnesium ion: 341, 342, 359

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
40% identity, 96% coverage: 11:368/372 of query aligns to 3:360/369 of P19566

query
sites
P19566

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H

L

L

P

P

A

S

N

D

-

I

T

A

D

D

G

V

A

T

L

L

P

E

C

G

R

E

I

V

D

Q

A

V

Q

V

I
x
E

Y

Q

L

L

G

G

T

H

D

E

L

T

Q

Q

Y

I

Q

H

V

I

S

Q

L

I

N

P

D

A

G

I

S

R

R

Q

L

N

T

L

V

V

R

Y

T

R

P

Q

N

N

S

D

-

V

-

L

V

V

D

E

Q

E

S

G

L

A

R

T

F

F

A

A

V

I

G

G

-

L

-

P

S

E

Q

R

A

C

G

H

L

L

L

F

F

R

D

E

R

D

G

G

S

S

A

A

S

C

L86 (= L98) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P176) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D181) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
E306 (≠ I320) mutation to K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
41% identity, 93% coverage: 11:355/372 of query aligns to 2:345/374 of 2awnB

query
sites
2awnB

A

S

V

V

S

Q

I

L

R

Q

A

N

V

V

R

T

K

K

V

A

Y
x
W

G

G

D

E

P

V

H

-

S

-

G

-

P

-

V

V

A
x
V

L

S

K

K

S

D

I

I

D

N

L

L

D

D

I

I

R

H

D

E

N

G

E

E

F

F

F

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

F

T

P

I

T

T

Q

S

G

G

E

D

I

L

L

F

L

I

Y

G

G

E

E

K

N

R

I

M

A

N

D

D

R

T

P

P

Y

A

Q

E

R

R

P

G

V

V

N

G

T

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

I

V

A

A

E

E

N

N

L

M

A

S

F

F

G

G

L

L

E

K

S

L

H

A

P

G

M

A

G

K

K

K

V

E

L

V

S

-

K

-

A

-

Q

-

I

I

A

N

E

Q

R

R

V

V

R

N

E

Q

M

V

L

A

A

E

L

V

V

L

Q

Q

M

L

E

A

R

H

F

L

A

L

T

D

R

R

R

K

P

P

T

K

Q

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

P

A

H

E

P

P

K

S

V

V

L

F

L

L

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

Q

V

A

Q

M

M

R

R

E

I

E

E

L

I

K

S

A

R

I

L

Q

H

A

K

K

R

T

L

G

G

I

R

T

T

F

M

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

I

I

A

V

V

V

L

L

S

D

E

A

G

G

E

R

V

V

Q

A

Q

Q

V

V

G

G

R

K

P

P

E

L

D

E

I

L

Y

Y

E

H

R

Y

P

P

R

A

N

D

R

R

F

F

V

V

A

A

D

G

F

F

I

I

G

G

-

S

-

P

E

K

T

M

N

N

F

F

I

L

E

P

G

V

T

K

V

V

T

T

H

A

V

T

E

A

A

I

G

D

L

Q

A

V

W

Q

F

V

A

E

G

L

P

P

A

M

G

P

H

N

P

R

-

Q

-

V

-

W

L

L

P

P

A

V

Q

E

P

-

C

S

S

R

D

D

V

V

N

Q

V

V

G

G

A

A

T

N

V

M

A

S

L

L

S

G

V

I

R

R

P

P

E

E

R

-

L

-

H

H

L

L

P

P

A

S

N

D

-

I

T

A

D

D

G

V

A

I

L

L

P

E

C

G

R

E

I

V

D

Q

A

V

Q

V

I

E

Y

Q

L

L

G

G

T

N

D

E

L

T

Q

Q

Y

I

Q

H

V

I

S

Q

L

I

N

P

D

S

G

I

S

R

R

Q

L

N

T

L

V

V

R

Y

T

R

P

Q

N

N

S

D

-

V

-

L

V

V

D

E

Q

E

S

G

L

A

R

T

F

F

A

A

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y21), V17 (≠ A30), S37 (= S50), G38 (= G51), C39 (= C52), G40 (= G53), K41 (= K54), S42 (≠ T55), T43 (= T56)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
41% identity, 93% coverage: 11:355/372 of query aligns to 3:346/371 of P68187

query
sites
P68187

A

S

V

V

S

Q

I

L

R

Q

A

N

V

V

R

T

K

K

V

A

Y

W

G

G

D

E

P

V

H

-

S

-

G

-

P

-

V

V

A

V

L

S

K

K

S

D

I

I

D

N

L

L

D

D

I

I

R

H

D

E

N

G

E

E

F

F

F

V

T

V

L

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

F

T

P

I

T

T

Q

S

G

G

E

D

I

L

L

F

L

I

Y

G

G

E

E

K

N

R

I

M

A

N

D

D

R

T

P

P

Y

A

Q

E

R

R

P

G

V

V

N

G

T

M

V

V

F

F

Q

Q

H

S

Y

Y

A
|
A

L

L

F

Y

P

P

H

H

M

L

T

S

I

V

A

A

E

E

N

N

L

M

A

S

F

F

G

G

L

L

E

K

S

L

H

A

P

G

M

A

G

-

K

-

V

-

L

-

S
x
K

K

K

A

E

Q

V

I

I

A

N

E

Q

R

R

V
|
V

R

N

E

Q

M
x
V

L

A

A
x
E

L

V

V

L

Q

Q

M

L

E
x
A

R

H

F

L

A

L

T

D

R

R

R

K

P

P

T

K

Q

A

L

L

S

S

G

G

G
|
G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

P

A

H

E

P

P

K

S

V

V

L

F

L

L

D
|
D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

Q

V

A

Q

M

M

R

R

E

I

E

E

L

I

K

S

A

R

I

L

Q

H

A

K

K

R

T

L

G

G

I

R

T

T

F

M

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

I

I

A

V

V

V

L

L

S

D

E

A

G

G

E

R

V

V

Q

A

Q

Q

V

V

G

G

R

K

P

P

E

L

D

E

I

L

Y

Y

E

H

R

Y

P

P

R

A

N

D

R
|
R

F

F

V

V

A

A

D

G

F

F

I

I

G

G

-

S

-

P

E

K

T

M

N

N

F
|
F

I

L

E

P

G

V

T

K

V

V

T

T

H

A

V

T

E

A

A

I

G

D

L

Q

A

V

W

Q

F

V

A

E

G

L

P

P

A

M

G

P

H

N

P

R

-

Q

-

V

-
x
W

L

L

P

P

A

V

Q

E

P

-

C

S

S

R

D

D

V

V

N

Q

V

V

G
|
G

A

A

T

N

V

M

A
x
S

L

L

S
x
G

V

I

R

R

P

P

E

E

R

-

L

-

H

H

L

L

P

P

A

S

N

D

-

I

T

A

D

D

G

V

A

I

L

L

P

E

C
x
G

R

E

I

V

D

Q

A

V

Q

V

I
x
E

Y

Q

L

L

G

G

T

N

D

E

L

T

Q

Q

Y

I

Q

H

V

I

S

Q

L

I

N

P

D
x
S

G

I

S

R

R

Q

L

N

T

L

V

V

R

Y

T

R

P

Q

N

N

S

D

-

V

-

L

V

V

D

E

Q

E

S
x
G

L

A

R

T

F

F

A

A

V

I

G
|
G

A85 (= A97) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ S122) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V130) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ M133) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ A135) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ E140) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G153) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D174) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (= R244) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
F241 (= F255) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
W267 (vs. gap) mutation to G: Normal maltose transport but constitutive mal gene expression.
G278 (= G289) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
S282 (≠ A293) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G284 (≠ S295) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G302 (≠ C314) mutation to D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
E308 (≠ I320) mutation to Q: Maltose transport is affected but retains ability to interact with MalT.
S322 (≠ D334) mutation to F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G340 (≠ S349) mutation to A: Maltose transport is affected but retains ability to interact with MalT.
G346 (= G355) mutation to S: Normal maltose transport but constitutive mal gene expression.

Sites not aligning to the query:

355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
41% identity, 93% coverage: 11:355/372 of query aligns to 2:345/372 of 2awoA

query
sites
2awoA

A

S

V

V

S

Q

I

L

R

Q

A

N

V

V

R

T

K

K

V

A

Y
x
W

G

G

D

E

P

V

H

-

S

-

G

-

P

-

V

V

A
x
V

L

S

K

K

S

D

I

I

D

N

L

L

D

D

I

I

R

H

D

E

N

G

E

E

F

F

F

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

F

T

P

I

T

T

Q

S

G

G

E

D

I

L

L

F

L

I

Y

G

G

E

E

K

N

R

I

M

A

N

D

D

R

T

P

P

Y

A

Q

E

R

R

P

G

V

V

N

G

T

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

I

V

A

A

E

E

N

N

L

M

A

S

F

F

G

G

L

L

E

K

S

L

H

A

P

G

M

A

G

-

K

-

V

-

L

-

S

K

K

K

A

E

Q

V

I

I

A

N

E

Q

R

R

V

V

R

N

E

Q

M

V

L

A

A

E

L

V

V

L

Q

Q

M

L

E

A

R

H

F

L

A

L

T

D

R

R

R

K

P

P

T

K

Q

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

P

A

H

E

P

P

K

S

V

V

L

F

L

L

D
|
D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

Q

V

A

Q

M

M

R

R

E

I

E

E

L

I

K

S

A

R

I

L

Q

H

A

K

K

R

T

L

G

G

I

R

T

T

F

M

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

I

I

A

V

V

V

L

L

S

D

E

A

G

G

E

R

V

V

Q

A

Q

Q

V

V

G

G

R

K

P

P

E

L

D

E

I

L

Y

Y

E

H

R

Y

P

P

R

A

N

D

R

R

F

F

V

V

A

A

D

G

F

F

I

I

G

G

-

S

-

P

E

K

T

M

N

N

F

F

I

L

E

P

G

V

T

K

V

V

T

T

H

A

V

T

E

A

A

I

G

D

L

Q

A

V

W

Q

F

V

A

E

G

L

P

P

A

M

G

P

H

N

P

R

-

Q

-

V

-

W

L

L

P

P

A

V

Q

E

P

-

C

S

S

R

D

D

V

V

N

Q

V

V

G

G

A

A

T

N

V

M

A

S

L

L

S

G

V

I

R

R

P

P

E

E

R

-

L

-

H

H

L

L

P

P

A

S

N

D

-

I

T

A

D

D

G

V

A

I

L

L

P

E

C

G

R

E

I

V

D

Q

A

V

Q

V

I

E

Y

Q

L

L

G

G

T

N

D

E

L

T

Q

Q

Y

I

Q

H

V

I

S

Q

L

I

N

P

D

S

G

I

S

R

R

Q

L

N

T

L

V

V

R

Y

T

R

P

Q

N

N

S

D

-

V

-

L

V

V

D

E

Q

E

S

G

L

A

R

T

F

F

A

A

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y21), V17 (≠ A30), S37 (= S50), G38 (= G51), C39 (= C52), G40 (= G53), K41 (= K54), S42 (≠ T55), T43 (= T56)
binding magnesium ion: S42 (≠ T55), D157 (= D174)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
41% identity, 93% coverage: 11:355/372 of query aligns to 2:345/371 of 3puyA

query
sites
3puyA

A

S

V

V

S

Q

I

L

R

Q

A

N

V

V

R

T

K

K

V

A

Y
x
W

G

G

D

E

P

V

H

-

S

-

G

-

P

-

V

V

A
x
V

L

S

K

K

S

D

I

I

D

N

L

L

D

D

I

I

R

H

D

E

N

G

E

E

F

F

F

V

T

V

L

F

L

V

G

G

P
|
P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

F

T

P

I

T

T

Q

S

G

G

E

D

I

L

L

F

L

I

Y

G

G

E

E

K

N

R

I

M

A

N

D

D

R

T

P

P

Y

A

Q

E

R

R

P

G

V

V

N

G

T

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

I

V

A

A

E

E

N

N

L

M

A

S

F

F

G

G

L

L

E

K

S

L

H

A

P

G

M

A

G

-

K

-

V

-

L

-

S

K

K

K

A

E

Q

V

I

I

A

N

E

Q

R

R

V

V

R

N

E

Q

M

V

L

A

A

E

L

V

V

L

Q

Q

M

L

E

A

R

H

F

L

A

L

T

D

R
|
R

R

K

P

P

T

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

P

A

H

E

P

P

K

S

V

V

L

F

L

L

D
|
D

E
|
E

P

P

L

L

S

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

Q

V

A

Q

M

M

R

R

E

I

E

E

L

I

K

S

A

R

I

L

Q

H

A

K

K

R

T

L

G

G

I

R

T

T

F

M

I

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

I

I

A

V

V

V

L

L

S

D

E

A

G

G

E

R

V

V

Q

A

Q

Q

V

V

G

G

R

K

P

P

E

L

D

E

I

L

Y

Y

E

H

R

Y

P

P

R

A

N

D

R

R

F

F

V

V

A

A

D

G

F

F

I

I

G

G

-

S

-

P

E

K

T

M

N

N

F

F

I

L

E

P

G

V

T

K

V

V

T

T

H

A

V

T

E

A

A

I

G

D

L

Q

A

V

W

Q

F

V

A

E

G

L

P

P

A

M

G

P

H

N

P

R

-

Q

-

V

-

W

L

L

P

P

A

V

Q

E

P

-

C

S

S

R

D

D

V

V

N

Q

V

V

G

G

A

A

T

N

V

M

A

S

L

L

S

G

V

I

R

R

P

P

E

E

R

-

L

-

H

H

L

L

P

P

A

S

N

D

-

I

T

A

D

D

G

V

A

I

L

L

P

E

C

G

R

E

I

V

D

Q

A

V

Q

V

I

E

Y

Q

L

L

G

G

T

N

D

E

L

T

Q

Q

Y

I

Q

H

V

I

S

Q

L

I

N

P

D

S

G

I

S

R

R

Q

L

N

T

L

V

V

R

Y

T

R

P

Q

N

N

S

D

-

V

-

L

V

V

D

E

Q

E

S

G

L

A

R

T

F

F

A

A

V

I

G

G

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y21), V17 (≠ A30), P36 (= P49), S37 (= S50), G38 (= G51), C39 (= C52), G40 (= G53), K41 (= K54), S42 (≠ T55), T43 (= T56), Q81 (= Q94), R128 (= R145), A132 (≠ Q149), S134 (= S151), G135 (= G152), G136 (= G153), Q137 (= Q154), E158 (= E175), H191 (= H208)
binding magnesium ion: S42 (≠ T55), Q81 (= Q94), D157 (= D174)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
41% identity, 93% coverage: 11:355/372 of query aligns to 2:345/371 of 3puxA

query
sites
3puxA

A

S

V

V

S

Q

I

L

R

Q

A

N

V

V

R

T

K

K

V

A

Y
x
W

G

G

D

E

P

V

H

-

S

-

G

-

P

-

V

V

A
x
V

L

S

K

K

S

D

I

I

D

N

L

L

D

D

I

I

R

H

D

E

N

G

E

E

F

F

F

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

F

T

P

I

T

T

Q

S

G

G

E

D

I

L

L

F

L

I

Y

G

G

E

E

K

N

R

I

M

A

N

D

D

R

T

P

P

Y

A

Q

E

R

R

P

G

V

V

N

G

T

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

I

V

A

A

E

E

N

N

L

M

A

S

F

F

G

G

L

L

E

K

S

L

H

A

P

G

M

A

G

-

K

-

V

-

L

-

S

K

K

K

A

E

Q

V

I

I

A

N

E

Q

R

R

V

V

R

N

E

Q

M

V

L

A

A

E

L

V

V

L

Q

Q

M

L

E

A

R

H

F

L

A

L

T

D

R
|
R

R

K

P

P

T

K

Q
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

P

A

H

E

P

P

K

S

V

V

L

F

L

L

D
|
D

E
|
E

P

P

L

L

S

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

Q

V

A

Q

M

M

R

R

E

I

E

E

L

I

K

S

A

R

I

L

Q

H

A

K

K

R

T

L

G

G

I

R

T

T

F

M

I

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

I

I

A

V

V

V

L

L

S

D

E

A

G

G

E

R

V

V

Q

A

Q

Q

V

V

G

G

R

K

P

P

E

L

D

E

I

L

Y

Y

E

H

R

Y

P

P

R

A

N

D

R

R

F

F

V

V

A

A

D

G

F

F

I

I

G

G

-

S

-

P

E

K

T

M

N

N

F

F

I

L

E

P

G

V

T

K

V

V

T

T

H

A

V

T

E

A

A

I

G

D

L

Q

A

V

W

Q

F

V

A

E

G

L

P

P

A

M

G

P

H

N

P

R

-

Q

-

V

-

W

L

L

P

P

A

V

Q

E

P

-

C

S

S

R

D

D

V

V

N

Q

V

V

G

G

A

A

T

N

V

M

A

S

L

L

S

G

V

I

R

R

P

P

E

E

R

-

L

-

H

H

L

L

P

P

A

S

N

D

-

I

T

A

D

D

G

V

A

I

L

L

P

E

C

G

R

E

I

V

D

Q

A

V

Q

V

I

E

Y

Q

L

L

G

G

T

N

D

E

L

T

Q

Q

Y

I

Q

H

V

I

S

Q

L

I

N

P

D

S

G

I

S

R

R

Q

L

N

T

L

V

V

R

Y

T

R

P

Q

N

N

S

D

-

V

-

L

V

V

D

E

Q

E

S

G

L

A

R

T

F

F

A

A

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y21), V17 (≠ A30), S37 (= S50), G38 (= G51), C39 (= C52), G40 (= G53), K41 (= K54), S42 (≠ T55), T43 (= T56), R128 (= R145), A132 (≠ Q149), L133 (= L150), S134 (= S151), Q137 (= Q154)
binding beryllium trifluoride ion: S37 (= S50), G38 (= G51), K41 (= K54), Q81 (= Q94), S134 (= S151), G135 (= G152), G136 (= G153), E158 (= E175), H191 (= H208)
binding magnesium ion: S42 (≠ T55), Q81 (= Q94), D157 (= D174)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
41% identity, 93% coverage: 11:355/372 of query aligns to 2:345/371 of 3puwA

query
sites
3puwA

A

S

V

V

S

Q

I

L

R

Q

A

N

V

V

R

T

K

K

V

A

Y
x
W

G

G

D

E

P

V

H

-

S

-

G

-

P

-

V

V

A
x
V

L

S

K

K

S

D

I

I

D

N

L

L

D

D

I

I

R

H

D

E

N

G

E

E

F

F

F

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

F

T

P

I

T

T

Q

S

G

G

E

D

I

L

L

F

L

I

Y

G

G

E

E

K

N

R

I

M

A

N

D

D

R

T

P

P

Y

A

Q

E

R

R

P

G

V

V

N

G

T

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

I

V

A

A

E

E

N

N

L

M

A

S

F

F

G

G

L

L

E

K

S

L

H

A

P

G

M

A

G

-

K

-

V

-

L

-

S

K

K

K

A

E

Q

V

I

I

A

N

E

Q

R

R

V

V

R

N

E

Q

M

V

L

A

A

E

L

V

V

L

Q

Q

M

L

E

A

R

H

F

L

A

L

T

D

R
|
R

R

K

P

P

T

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

P

A

H

E

P

P

K

S

V

V

L

F

L

L

D

D

E
|
E

P

P

L

L

S

S

A
x
N

L

L

D

D

L

A

K

A

L

L

R

R

Q

V

A

Q

M

M

R

R

E

I

E

E

L

I

K

S

A

R

I

L

Q

H

A

K

K

R

T

L

G

G

I

R

T

T

F

M

I

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

I

I

A

V

V

V

L

L

S

D

E

A

G

G

E

R

V

V

Q

A

Q

Q

V

V

G

G

R

K

P

P

E

L

D

E

I

L

Y

Y

E

H

R

Y

P

P

R

A

N

D

R

R

F

F

V

V

A

A

D

G

F

F

I

I

G

G

-

S

-

P

E

K

T

M

N

N

F

F

I

L

E

P

G

V

T

K

V

V

T

T

H

A

V

T

E

A

A

I

G

D

L

Q

A

V

W

Q

F

V

A

E

G

L

P

P

A

M

G

P

H

N

P

R

-

Q

-

V

-

W

L

L

P

P

A

V

Q

E

P

-

C

S

S

R

D

D

V

V

N

Q

V

V

G

G

A

A

T

N

V

M

A

S

L

L

S

G

V

I

R

R

P

P

E

E

R

-

L

-

H

H

L

L

P

P

A

S

N

D

-

I

T

A

D

D

G

V

A

I

L

L

P

E

C

G

R

E

I

V

D

Q

A

V

Q

V

I

E

Y

Q

L

L

G

G

T

N

D

E

L

T

Q

Q

Y

I

Q

H

V

I

S

Q

L

I

N

P

D

S

G

I

S

R

R

Q

L

N

T

L

V

V

R

Y

T

R

P

Q

N

N

S

D

-

V

-

L

V

V

D

E

Q

E

S

G

L

A

R

T

F

F

A

A

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y21), V17 (≠ A30), S37 (= S50), G38 (= G51), C39 (= C52), G40 (= G53), K41 (= K54), S42 (≠ T55), T43 (= T56), R128 (= R145), A132 (≠ Q149), S134 (= S151), Q137 (= Q154)
binding tetrafluoroaluminate ion: S37 (= S50), G38 (= G51), K41 (= K54), Q81 (= Q94), S134 (= S151), G135 (= G152), G136 (= G153), E158 (= E175), N162 (≠ A179), H191 (= H208)
binding magnesium ion: S42 (≠ T55), Q81 (= Q94)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
41% identity, 92% coverage: 12:355/372 of query aligns to 1:343/367 of 1q12A

query
sites
1q12A

V

V

S

Q

I

L

R

Q

A

N

V

V

R

T

K

K

V

A

Y
x
W

G

G

D

E

P

V

H

-

S

-

G

-

P

-

V

V

A
x
V

L

S

K

K

S

D

I

I

D

N

L

L

D

D

I

I

R

H

D

E

N

G

E

E

F

F

F

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

F

T

P

I

T

T

Q

S

G

G

E

D

I

L

L

F

L

I

Y

G

G

E

E

K

N

R

I

M

A

N

D

D

R

T

P

P

Y

A

Q

E

R

R

P

G

V

V

N

G

T

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

I

V

A

A

E

E

N

N

L

M

A

S

F

F

G

G

L

L

E

K

S

L

H

A

P

G

M

A

G

-

K

-

V

-

L

-

S

K

K

K

A

E

Q

V

I

I

A

N

E

Q

R

R

V

V

R

N

E

Q

M

V

L

A

A

E

L

V

V

L

Q

Q

M

L

E

A

R

H

F

L

A

L

T

D

R
|
R

R

K

P

P

T
x
K

Q
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

P

A

H

E

P

P

K

S

V

V

L

F

L

L

D

D

E

E

P

P

L

L

S

S

A
x
N

L

L

D

D

L

A

K

A

L

L

R

R

Q

V

A

Q

M

M

R

R

E

I

E

E

L

I

K

S

A

R

I

L

Q

H

A

K

K

R

T

L

G

G

I

R

T

T

F

M

I

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

I

I

A

V

V

V

L

L

S

D

E

A

G

G

E

R

V

V

Q

A

Q

Q

V

V

G

G

R

K

P

P

E

L

D

E

I

L

Y

Y

E

H

R

Y

P

P

R

A

N

D

R

R

F

F

V

V

A

A

D

G

F

F

I

I

G

G

-

S

-

P

E

K

T

M

N

N

F

F

I

L

E

P

G

V

T

K

V

V

T

T

H

A

V

T

E

A

A

I

G

D

L

Q

A

V

W

Q

F

V

A

E

G

L

P

P

A

M

G

P

H

N

P

R

-

Q

-

V

-

W

L

L

P

P

A

V

Q

E

P

-

C

S

S

R

D

D

V

V

N

Q

V

V

G

G

A

A

T

N

V

M

A

S

L

L

S

G

V

I

R

R

P

P

E

E

R

-

L

-

H

H

L

L

P

P

A

S

N

D

-

I

T

A

D

D

G

V

A

I

L

L

P

E

C

G

R

E

I

V

D

Q

A

V

Q

V

I

E

Y

Q

L

L

G

G

T

N

D

E

L

T

Q

Q

Y

I

Q

H

V

I

S

Q

L

I

N

P

D

S

G

I

S

R

R

Q

L

N

T

L

V

V

R

Y

T

R

P

Q

N

N

S

D

-

V

-

L

V

V

D

E

Q

E

S

G

L

A

R

T

F

F

A

A

V

I

G

G

binding adenosine-5'-triphosphate: W10 (≠ Y21), V15 (≠ A30), S35 (= S50), G36 (= G51), C37 (= C52), G38 (= G53), K39 (= K54), S40 (≠ T55), T41 (= T56), R126 (= R145), K129 (≠ T148), A130 (≠ Q149), L131 (= L150), S132 (= S151), G133 (= G152), G134 (= G153), Q135 (= Q154), N160 (≠ A179), H189 (= H208)

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
45% identity, 82% coverage: 30:334/372 of query aligns to 21:313/353 of 1vciA

query
sites
1vciA

A

A

L

V

K

N

S

K

I

L

D

N

L

L

D

T

I

I

R

K

D

D

N

G

E

E

F

F

F

L

T

V

L

L

G

G

P
|
P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

L

T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

F

E

P

P

T

T

Q

E

G

G

E

R

I

I

L

Y

L

F

Y

G

G

D

E

R

N

D

I

V

A

T

D

Y

R

L

P

P

Y

K

Q

D

R

R

P

N

V

I

N

S

T

M

V

V

F

F

Q

Q

H

H

Y

-

A

-

L

-

F

-

P

-

H

-

M

M

T

T

I

V

A

Y

E

E

N

N

L

I

A

A

F

F

G

P

L

L

E

K

S

K

H

F

P

P

M

-

G

-

K

-

V

-

L

-

S

-

K

K

A

D

Q

E

I

I

A

D

E

K

R

R

V

V

R

R

E

W

M

A

L

A

A

E

L

L

V

L

Q

Q

M

I

E

E

R

E

F

L

A

L

T

N

R

R

R

Y

P

P

T

A

Q

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

V

A

A

R

R

A

A

L

I

A

V

P

V

H

E

P

P

K

D

V

V

L

L

L

M

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

L

A

K

K

L

L

R

R

Q

V

A

A

M

M

R

R

E

A

E

E

L

I

K

K

A

K

I

L

Q

Q

A

Q

K

K

T

L

G

K

I

V

T

T

F

T

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

M

S

G

D

D

R

R

I

I

A

A

V

V

L

M

S

N

E

R

G

G

E

Q

V

L

Q

L

Q

Q

V

I

G

G

R

S

P

P

E

T

D

E

I

V

Y

Y

E

L

R

R

P

P

R

N

N

S

R

V

F

F

V

V

A

A

D

T

F

F

I

I

G

G

-

A

-

P

E

E

T

M

N

N

F

I

I

L

E

E

G

-

T

-

V

-

T

-

H

-

V

V

E

S

A

V

G

G

L

D

A

G

W

Y

F

L

A

E

G

G

P

R

A

G

G

F

H

R

-

I

P

E

L

L

P

P

A

Q

Q

M

P

D

C

L

S

L

D

K

V

D

N

Y

V

V

G

G

A

K

T

T

V

V

A

L

L

F

S

G

V

I

R

R

P

P

E

E

R

H

L

M

H

T

L

V

-

E

-

G

L

V

P

H

A

M

N

K

T

R

D

T

G

A

A

R

L

L

P

I

C

G

R

K

I

V

D

D

A

F

Q

V

I

E

Y

A

L

L

G

G

T

T

D

D

L

T

Q

I

Y

L

Q

H

V

V

S

K

L

F

N

G

D

D

binding adenosine-5'-triphosphate: P40 (= P49), S41 (= S50), G42 (= G51), C43 (= C52), G44 (= G53), K45 (= K54), T46 (= T55), T47 (= T56)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 16, 19

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
43% identity, 85% coverage: 17:333/372 of query aligns to 9:309/393 of P9WQI3

query
sites
P9WQI3

V

V

R

N

K

K

V

S

Y

Y

G

P

D

D

P

G

H

H

S

T

G

-

P

-

V

-

A

A

L

V

K

R

S

D

I

L

D

N

L

L

D

T

I

I

R

A

D

D

N

G

E

E

F

F

F

L

T

I

L

L

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

T

L

T

L

L

R

N

M

M

I

I

A

A

G

G

F

L

E

E

F

D

P

I

T

S

Q

S

G

G

E

E

I

L

L

R

L

I

Y

A

G

G

E

E

N

R

I

V

A

N

D

E

R

K

P

A

P

P

Y

K

Q

D

R

R

P

D

V

I

N

A

T

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

I

V

A

R

E

Q

N

N

L

I

A

A

F

F

G

P

L

L

E

T

S

L

H

A

P

K

M

M

G

-

K

-

V

-

L

-

S

R

K

K

A

A

Q

D

I

I

A

A

E

Q

R

K

V

V

R

S

E

E

M

T

L

A

A

K

L

I

V

L

Q

D

M

L

E

T

R

N

F

L

A

L

T

D

R

R

R

K

P

P

T

S

Q

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

A

V

P

R

H

H

P

P

K

K

V

A

L

F

L

L

L

M

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

L

A

K

K

L

L

R

R

Q

V

A

Q

M

M

R

R

E

G

E

E

L

I

K

A

A

Q

I

L

Q

Q

A

R

K

R

T

L

G

G

I

T

T

T

F

T

I

V

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

T

E

E

A

A

L

M

T

T

M

L

S

G

D

D

R

R

I

V

A

V

V

V

L

M

S

Y

E

G

G

G

E

I

V

A

Q

Q

V

I

G

G

R

T

P

P

E

E

D

E

I

L

Y

Y

E

E

R

R

P

P

R

A

N

N

R

L

F

F

V

V

A

A

D

G

F

F

I

I

G

G

E

S

-

P

-

A

T

M

N

N

F

F

I

F

E

P

G

A

T

R

V

L

T

T

H

A

V

I

E

-

A

-

G

G

L

L

A

T

W

L

F

P

A

F

G

G

P

E

-

V

-

T

-

L

-

A

-

P

-

E

-

V

-

Q

-

G

-

V

-

I

A

A

G

A

H

H

P

P

L

K

P

P

A

-

Q

-

P

-

C

-

S

-

D

-

V

-

N

-

V

-

G

-

A

E

T

N

V

V

A

I

L

V

S

G

V

V

R

R

P

P

E

E

R

H

L

I

H

Q

L

-

P

-

A

-

N

-

T

-

D

D

G

A

A

A

L

L

P

-

C

-

R

-

I

I

D

D

A

A

Q

Y

I

Q

Y

R

L

I

G

R

T

A

D

-

L

L

Q

T

Y

F

Q

Q

V

V

S

K

L

V

N

N

H193 (= H208) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

7cagC Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (see paper)
48% identity, 64% coverage: 25:263/372 of query aligns to 10:246/362 of 7cagC

query
sites
7cagC

H

K

S

S

G

Y

P

P

V
x
A

A

A

L

V

K

K

S

E

I

F

D

S

L

M

D

T

I

I

R

A

D

D

N

G

E
&nb