SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO353_25430 FitnessBrowser__pseudo3_N2E3:AO353_25430 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
28% identity, 58% coverage: 73:228/268 of query aligns to 87:247/296 of P68183

query
sites
P68183

N

N

S

S

L

V

L

K

I

V

A

A

V

G

C

I

A

S

S

A

M

I

I

G

A

I

T

V

A

A

I

L

A

S

T

T

L

T

A

C

A

A

L

Y

G

A

T

F

S

A

R

R

G

-

A

M

K

R

F

F

K

P

G

G

M

K

Q

A

L

T

S

L

M

L

G

K

A

G

I

M

M

L

L

I

P

F

L

Q

V

M

L

F

P

P

E

A

I

V

V

L

V

S

G

L

V

V

A

A

T

L

L

Y

A

A

L

L

F

F

S

D

T

R

V

L

G

G

L

-

S

-

L

-

G

E

Y

Y

G

I

N

P

L

F

I

I

I

G

A

L

H

N

T

T

V

H

F

G

C

G

I

V

P

I

F

F

A

A

Y

Y

L

L

-

G

-

G

-

I

-

A

-

L

-

H

-

V

-

W

P

T

I

I

R

K

A

G

R

Y

L

F

N

E

D

T

M

I

D

D

L

S

S

S

L

L

E

E

Q
x
E

A

A

S
x
A

A

A

D

L

L

D

Y
x
G

A

A

G

T

P

P

W

W

R

Q

T

A

F

F

R

R

K

L

V

V

T

L

L

L

P
|
P

L

L

L

S

M

V

P

P

G

I

I

L

V

A

S

V

G

V

L

F

M

I

L

L

A

S

F

F

I

I

V

A

S

A

L

I

D

T

N

E

F

V

-

P

V

V

I

A

S

S

M

L

M

L

V

L

A

R

Q

D

A

V

G

N

T

S

T

Y

T

T

L

L

P

A

I

V

E190 (≠ Q172) mutation E->A,C,K,L: Reduction of transport rate.
A192 (≠ S174) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (≠ Y178) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P191) mutation to A: No effect.

Query Sequence

>AO353_25430 FitnessBrowser__pseudo3_N2E3:AO353_25430
MQRLFKRTGLSVQDFPGFGGFSFLFYLYLYAPIVVLVVFSFNANQSATVWTGLSLDWYRA
AFANQALRQAAGNSLLIAVCASMIATAIATLAALGTSRGAKFKGMQLSMGAIMLPLVLPE
IVVGVATLALFSTVGLSLGYGNLIIAHTVFCIPFAYLPIRARLNDMDLSLEQASADLYAG
PWRTFRKVTLPLLMPGIVSGLMLAFIVSLDNFVISMMVAQAGTTTLPIFIFGLLRMGVTP
DVNAVSTLILGVSVLFVSLSYLLGKKKT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory