SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO353_25660 FitnessBrowser__pseudo3_N2E3:AO353_25660 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P02911 Lysine/arginine/ornithine-binding periplasmic protein; LAO-binding protein from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
42% identity, 100% coverage: 1:259/259 of query aligns to 1:260/260 of P02911

query
sites
P02911

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D33 (≠ N33) binding ; binding ; binding ; mutation to A: Decreases arginine and histidine binding affinity.
Y36 (= Y36) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
D52 (≠ G52) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
C60 (= C60) modified: Disulfide link with 67
C67 (= C67) modified: Disulfide link with 60
F74 (= F74) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
S91 (= S91) binding ; binding ; mutation to A: Has no effect on the arginine and histidine binding affinity.
S92 (= S92) binding ; binding ; binding ; mutation to A: Decreases arginine and histidine binding affinity.
S94 (≠ T94) binding ; binding ; binding ; mutation to A: Decreases arginine and histidine binding affinity.
R99 (= R99) binding ; binding ; binding ; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
L139 (≠ M139) mutation to K: Changes ligand specificity. Can bind glutamine. Still able to bind basic amino acids, however, with 1000-fold less affinity for arginine.; mutation to Q: Does not affect ligand preference.
T143 (≠ I143) binding ; binding ; binding
D183 (= D183) binding ; binding ; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.

P0AEU0 Histidine-binding periplasmic protein; HBP from Escherichia coli (strain K12) (see 3 papers)
43% identity, 89% coverage: 28:257/259 of query aligns to 28:258/260 of P0AEU0

query
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P0AEU0

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C60 (= C60) modified: Disulfide link with 67
C67 (= C67) modified: Disulfide link with 60
S91 (= S91) binding
S92 (= S92) binding
S94 (≠ T94) binding
R99 (= R99) binding
T143 (≠ I143) binding
D183 (= D183) binding

Sites not aligning to the query:

1:22 signal peptide

1lstA Three-dimensional structures of the periplasmic lysine-, arginine-, ornithine-binding protein with and without a ligand (see paper)
44% identity, 90% coverage: 28:259/259 of query aligns to 6:238/238 of 1lstA

query
sites
1lstA

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binding lysine: Y14 (= Y36), F52 (= F74), S70 (= S92), S72 (≠ T94), R77 (= R99), L117 (≠ M139), S120 (≠ T142), T121 (≠ I143), Q122 (= Q144), D161 (= D183)

1lahE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
44% identity, 90% coverage: 28:259/259 of query aligns to 6:238/238 of 1lahE

query
sites
1lahE

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binding L-ornithine: Y14 (= Y36), F52 (= F74), S70 (= S92), S72 (≠ T94), R77 (= R99), S120 (≠ T142), Q122 (= Q144), D161 (= D183)

1lagE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
44% identity, 90% coverage: 28:259/259 of query aligns to 6:238/238 of 1lagE

query
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1lagE

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L

L

H

R

E

Q

K

D

G

G

I

T

Y

Y

A

D

Q

K

I

M

Q

A

K

K

Y

Y

F

F

G

-

D

D

L

F

D

N

L

V

Y

Y

N

G

N

D

binding histidine: Y14 (= Y36), F52 (= F74), S69 (= S91), S70 (= S92), L71 (≠ M93), S72 (≠ T94), R77 (= R99), L117 (≠ M139), S120 (≠ T142), Q122 (= Q144), D161 (= D183)

1lafE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
44% identity, 90% coverage: 28:259/259 of query aligns to 6:238/238 of 1lafE

query
sites
1lafE

L

V

R

R

F

I

G

G

T

T

N
x
D

P

T

S

T

Y
|
Y

P

A

P

P

F

F

E

S

S

S

T

K

T

D

A

A

D

K

G

G

G

E

V

F

Q

I

G

G

F

F

G

D

V

I

D

D

L

L

G

G

N

N

A

E

I

M

C

C

A

K

E

R

L

M

K

Q

L

V

K

K

C

C

V

T

W

W

V

V

S

A

N

S

D

D

F
|
F

D

D

G

A

L

L

I

I

P

P

A

S

L

L

K

K

A

A

G

K

K

K

F

I

D

D

A

A

I

I

E

I

S
|
S

M

L

T
x
S

V

I

T

T

D

D

A

K

R
|
R

K

Q

Q

E

I

I

D

A

F

F

T

S

D

D

R

K

L

L

Y

Y

A

A

G

A

P

D

T

S

A

R

I

L

V

I

T

A

R

A

K

K

D

G

S

S

G

P

L

I

E

Q

P

P

T

T

A

L

E

E

S

S

L

L

K

K

G

G

K

K

T

H

I

V

G

G

F

V

M
x
L

Q

Q

G

G

T
x
S

I
x
T

Q
|
Q

E

E

T

A

Y

Y

A

A

R

N

A

D

K

N

L

W

A

R

P

T

G

K

G

G

V

V

K

D

L

V

R

V

A

A

Y

Y

Q

A

N

N

Q

Q

D

D

Q

L

V

I

Y

Y

A

S

D

D

L

L

V

T

Y

A

G

G

R

R

L

L

D

D

A

A

S

A

I

L

Q

Q

D
|
D

K

E

L

V

Q

A

A

A

Q

S

M

E

S

G

F

F

L

L

E

K

S

Q

P

P

Q

A

G

G

A

K

P

E

F

Y

K

A

N

F

S

A

-

G

Q

P

G

S

I

V

S

K

D

D

P

K

L

K

V

Y

P

F

A

G

D

D

-

G

I

T

A

G

I

V

G

G

V

L

R

R

K

K

D

D

N

D

D

T

E

E

L

L

K

K

G

A

M

A

L

F

N

D

T

K

A

A

I

L

K

T

A

E

L

L

H

R

E

Q

K

D

G

G

I

T

Y

Y

A

D

Q

K

I

M

Q

A

K

K

Y

Y

F

F

G

-

D

D

L

F

D

N

L

V

Y

Y

N

G

N

D

binding arginine: D11 (≠ N33), Y14 (= Y36), F52 (= F74), S69 (= S91), S70 (= S92), S72 (≠ T94), R77 (= R99), L117 (≠ M139), S120 (≠ T142), T121 (≠ I143), Q122 (= Q144), D161 (= D183)

1hslA Refined 1.89 angstroms structure of the histidine-binding protein complexed with histidine and its relationship with many other active transport(slash)chemosensory receptors (see paper)
42% identity, 89% coverage: 27:257/259 of query aligns to 5:236/238 of 1hslA

query
sites
1hslA

E

K

L

I

R

R

F

I

G

G

T

T

N

D

P

P

S

T

Y
|
Y

P

A

P

P

F

F

E

E

S

S

T

K

T

N

A

A

D

Q

G

G

G

E

V

L

Q

V

G

G

F

F

G

D

V

I

D

D

L

L

G

A

N

K

A

E

I

L

C

C

A

K

E

R

L

I

K

N

L

T

K

Q

C

C

V

T

W

F

V

V

S

E

N

N

D

P

F
x
L

D

D

G

A

L

L

I

I

P

P

A

S

L

L

K

K

A

A

G

K

K

K

F

I

D

D

A

A

I

I

E

M

S
|
S

M
x
L

T
x
S

V

I

T

T

D

E

A

K

R
|
R

K

Q

Q

E

I

I

D

A

F

F

T

T

D

D

R

K

L

L

Y

Y

A

A

G

A

P

D

T

S

A

R

I

L

V

V

T

V

R

A

K

K

D

N

S

S

G

D

L

I

E

Q

P

P

T

T

A

V

E

A

S

S

L

L

K

K

G

G

K

K

T

R

I

V

G

G

F

V

M

L

Q

Q

G

G

T
|
T

I
x
T

Q

Q

E

E

T

T

Y

F

A

G

R

N

A

E

K

H

L

W

A

A

P

P

G

K

G

G

V

I

K

E

L

I

R

V

A

S

Y

Y

Q

Q

N

G

Q

Q

D

D

Q

N

V

I

Y

Y

A

S

D

D

L

L

V

T

Y

A

G

G

R

R

L

I

D

D

A

A

S

A

I

F

Q

Q

D
|
D

K

E

L

V

Q

A

A

A

Q

S

M

E

S

G

F

F

L

L

E

K

S

Q

P

P

Q

V

G

G

A

K

P

D

F

Y

K

K

-

F

N

G

S

G

Q

P

G

A

I

V

S

K

D

D

P

E

-

K

L

L

V

F

P

G

A

V

D

G

I

T

A

G

I

M

G

G

V

L

R

R

K

K

D

E

N

D

D

N

E

E

L

L

K

R

G

E

M

A

L

L

N

N

T

K

A

A

I

F

K

A

A

E

L

M

H

R

E

A

K

D

G

G

I

T

Y

Y

A

E

Q

K

I

L

Q

A

K

K

Y

Y

F

F

G

-

D

D

L

F

D

D

L

V

Y

Y

binding histidine: Y14 (= Y36), L52 (≠ F74), S69 (= S91), S70 (= S92), L71 (≠ M93), S72 (≠ T94), R77 (= R99), T120 (= T142), T121 (≠ I143), D161 (= D183)

P02910 Histidine-binding periplasmic protein; HBP from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
42% identity, 89% coverage: 27:257/259 of query aligns to 27:258/260 of P02910

query
sites
P02910

E

K

L

I

R

R

F

I

G

G

T

T

N

D

P

P

S

T

Y

Y

P

A

P

P

F

F

E
|
E

S

S

T
x
K

T

N

A

A

D

Q

G

G

G
x
E

V

L

Q

V

G

G

F

F

G

D

V

I

D

D

L

L

G

A

N

K

A

E

I

L

C

C

A

K

E

R

L

I

K

N

L

T

K

Q

C

C

V

T

W

F

V

V

S

E

N

N

D

P

F

L

D

D

G

A

L

L

I

I

P

P

A

S

L

L

K

K

A

A

G

K

K

K

F

I

D

D

A

A

I

I

E

M

S

S

M

L

T

S

V

I

T

T

D

E

A

K

R

R

K

Q

Q

E

I

I

D

A

F

F

T

T

D

D

R

K

L

L

Y

Y

A

A

G

A

P

D

T

S

A

R

I

L

V

V

T

V

R

A

K

K

D

N

S

S

G

D

L

I

E

Q

P

P

T

T

A

V

E

A

S

S

L

L

K

K

G

G

K

K

T

R

I

V

G

G

F

V

M

L

Q

Q

G

G

T

T

I

T

Q

Q

E

E

T

T

Y

F

A

G

R

N

A

E

K

H

L

W

A

A

P

P

G

K

G

G

V

I

K

E

L

I

R

V

A

S

Y

Y

Q

Q

N

G

Q

Q

D

D

Q

N

V

I

Y

Y

A

S

D
|
D

L

L

V

T

Y

A

G

G

R
|
R

L

I

D
|
D

A

A

S

A

I

F

Q

Q

D

D

K

E

L

V

Q

A

A

A

Q

S

M

E

S

G

F

F

L

L

E

K

S

Q

P

P

Q

V

G

G

A

K

P

D

F

Y

K

K

-

F

N

G

S

G

Q

P

G

A

I

V

S

K

D

D

P

E

-

K

L

L

V

F

P

G

A

V

D

G

I

T

A

G

I

M

G

G

V

L

R

R

K

K

D

E

N

D

D

N

E

E

L

L

K

R

G

E

M

A

L

L

N

N

T

K

A

A

I

F

K

A

A

E

L

M

H

R

E

A

K

D

G

G

I

T

Y

Y

A

E

Q

K

I

L

Q

A

K

K

Y

Y

F

F

G

-

D

D

L

F

D

D

L

V

Y

Y

E40 (= E40) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-47.
K42 (≠ T42) mutation to E: Increases ATPase-inducing activity and histidine transport; when associated with K-47.
E47 (≠ G47) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-40. Increases ATPase-inducing activity and histidine transport; when associated with E-42.
D171 (= D171) mutation D->A,N: Strong decrease in ATPase-inducing activity and histidine transport.
R176 (= R176) mutation R->D,S: Strong decrease in ATPase-inducing activity and histidine transport.
D178 (= D178) mutation to A: Slight decrease in ATPase-inducing activity and histidine transport.

Sites not aligning to the query:

1:22 signal peptide

5owfA Structure of a lao-binding protein mutant with glutamine (see paper)
44% identity, 90% coverage: 28:259/259 of query aligns to 3:235/235 of 5owfA

query
sites
5owfA

L

V

R

R

F

I

G

G

T

T

N

D

P

T

S

T

Y
|
Y

P

A

P

P

F

F

E

S

S

S

T

K

T

D

A

A

D

K

G

G

G

E

V

F

Q

I

G

G

F

F

G

D

V

I

D

D

L

L

G

G

N

N

A

E

I

M

C

C

A

K

E

R

L

M

K

Q

L

V

K

K

C

C

V

T

W

W

V

V

S

A

N

S

D

D

F
|
F

D

D

G

A

L

L

I

I

P

P

A

S

L

L

K

K

A

A

G

K

K

K

F

I

D

D

A

A

I

I

E

I

S
|
S

M

L

T
x
S

V

I

T

T

D

D

A

K

R
|
R

K

Q

Q

E

I

I

D

A

F

F

T

S

D

D

R

K

L

L

Y

Y

A

A

G

A

P

D

T

S

A

R

I

L

V

I

T

A

R

A

K

K

D

G

S

S

G

P

L

I

E

Q

P

P

T

T

A

L

E

E

S

S

L

L

K

K

G

G

K

K

T

H

I

V

G

G

F

V

M
x
K

Q

Q

G

G

T
x
S

I
x
T

Q

Q

E

E

T

A

Y

Y

A

A

R

N

A

D

K

N

L

W

A

R

P

T

G

K

G

G

V

V

K

D

L

V

R

V

A

A

Y

Y

Q

A

N

N

Q

Q

D

D

Q

L

V

I

Y

Y

A

S

D

D

L

L

V

T

Y

A

G

G

R

R

L

L

D

D

A

A

S

A

I

L

Q

Q

D
|
D

K

E

L

V

Q

A

A

A

Q

S

M

E

S

G

F

F

L

L

E

K

S

Q

P

P

Q

A

G

G

A

K

P

E

F

Y

K

A

N

F

S

A

-

G

Q

P

G

S

I

V

S

K

D

D

P

K

L

K

V

Y

P

F

A

G

D

D

-

G

I

T

A

G

I

V

G

G

V

L

R

R

K

K

D

D

N

D

D

T

E

E

L

L

K

K

G

A

M

A

L

F

N

D

T

K

A

A

I

L

K

T

A

E

L

L

H

R

E

Q

K

D

G

G

I

T

Y

Y

A

D

Q

K

I

M

Q

A

K

K

Y

Y

F

F

G

-

D

D

L

F

D

N

L

V

Y

Y

N

G

N

D

binding glutamine: Y11 (= Y36), F49 (= F74), S66 (= S91), S67 (= S92), S69 (≠ T94), R74 (= R99), K114 (≠ M139), S117 (≠ T142), T118 (≠ I143), D158 (= D183)

4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
34% identity, 87% coverage: 28:252/259 of query aligns to 6:226/226 of 4zv1A

query
sites
4zv1A

L

L

R

R

F

V

G

G

T

T

N
x
E

P

A

S

T

Y
x
F

P

P

F

F

E

G

S

F

T

K

T

D

A

E

D

N

G

G

G

K

V

L

Q

V

G

G

F

F

G

D

V

I

D

D

L

L

G

A

N

K

A

A

I

I

C

A

A

K

E

K

L

L

K

G

L

V

K

K

C

V

V

E

W

F

V

K

S

P

N

M

D

D

F
|
F

D

D

G

G

L

I

I

I

P

P

A

A

L

L

K

Q

A

S

G

G

K

K

F

I

D

D

A

V

I

V

E

I

S
x
A

S
x
G

M
|
M

T
|
T

V

I

T

T

D

E

A

E

R
|
R

K

K

Q

Q

I

V

D

D

F

F

T

S

D

D

R

P

L

Y

Y

F

A

E

G

A

P

G

T

Q

A

A

I

I

V

V

T

V

R

K

K

K

D

G

S

N

G

D

L

S

E

I

P

K

T

S

A

L

E

E

S

D

L

L

K

K

G

G

K

K

T

K

I

V

G

G

F

V

M
x
Q

Q

L

G

G

T
x
S

I
x
T

Q

S

E

E

T

Q

Y

H

A

V

R

K

A

K

K

V

L

A

A

K

P

D

G

A

G

G

V

V

K

K

L

V

R

K

A

K

Y

F

Q

D

N

N

Q

F

D

S

Q

E

V

A

Y

F

A

Q

D

E

L

L

V

K

Y

S

G

G

R

R

L

V

D

D

A

A

S

V

I

V

Q

T

D
|
D

K

N

L

A

Q

V

A

A

Q

L

M

A

S

Y

F

V

L

K

E

Q

S

N

P

P

Q

N

G

A

A

G

P

-

F

-

K

-

N

-

S

V

Q

K

G

I

I

V

S

G

D

E

P

T

L

F

V

S

P

G

A

E

D

P

I

Y

A

G

I

I

G

A

V

V

R

R

K

K

D

G

N

N

D

S

E

E

L

L

K

L

G

E

M

K

L

I

N

N

T

K

A

A

I

L

K

E

A

E

L

M

H

K

E

K

K

D

G

G

I

T

Y

Y

A

D

Q

K

I

I

Q

Y

K

E

K

K

Y

W

F

F

G

G

binding arginine: E11 (≠ N33), F14 (≠ Y36), F52 (= F74), A69 (≠ S91), G70 (≠ S92), M71 (= M93), T72 (= T94), R77 (= R99), Q117 (≠ M139), S120 (≠ T142), T121 (≠ I143), D161 (= D183)

4zv2A An ancestral arginine-binding protein bound to glutamine (see paper)
34% identity, 87% coverage: 28:253/259 of query aligns to 6:225/225 of 4zv2A

query
sites
4zv2A

L

L

R

R

F

V

G

G

T

T

N
x
E

P

A

S

T

Y
x
F

P

P

F

F

E

G

S

F

T

K

T

D

A

E

D

N

G

G

G

K

V

L

Q

V

G

G

F

F

G

D

V

I

D

D

L

L

G

A

N

K

A

A

I

I

C

A

A

K

E

K

L

L

K

G

L

V

K

K

C

V

V

E

W

F

V

K

S

P

N

M

D

D

F
|
F

D

D

G

G

L

I

I

I

P

P

A

A

L

L

K

Q

A

S

G

G

K

K

F

I

D

D

A

V

I

V

E

I

S

A

S
x
G

M
|
M

T
|
T

V

I

T

T

D

E

A

E

R
|
R

K

K

Q

Q

I

V

D

D

F

F

T

S

D

D

R

P

L

Y

Y

F

A

E

G

A

P

G

T

Q

A

A

I

I

V

V

T

V

R

K

K

K

D

G

S

N

G

D

L

S

E

I

P

K

T

S

A

L

E

E

S

D

L

L

K

K

G

G

K

K

T

K

I

V

G

G

F

V

M

Q

Q

L

G

G

T
x
S

I
x
T

Q

S

E

E

T

Q

Y

H

A

V

R

K

A

K

K

V

L

A

A

A

P

-

G

-

G

G

V

V

K

K

L

V

R

K

A

K

Y

F

Q

D

N

N

Q

F

D

S

Q

E

V

A

Y

F

A

Q

D

E

L

L

V

K

Y

S

G

G

R

R

L

V

D

D

A

A

S

V

I

V

Q

T

D
|
D

K

N

L

A

Q

V

A

A

Q

L

M

A

S

Y

F

V

L

K

E

Q

S

N

P

P

Q

N

G

A

A

G

P

-

F

-

K

-

N

-

S

V

Q

K

G

I

I

V

S

G

D

E

P

T

L

F

V

S

P

G

A

E

D

P

I

Y

A

G

I

I

G

A

V

V

R

R

K

K

D

G

N

N

D

S

E

E

L

L

K

L

G

E

M

K

L

I

N

N

T

K

A

A

I

L

K

E

A

E

L

M

H

K

E

K

K

D

G

G

I

T

Y

Y

A

D

Q

K

I

I

Q

Y

K

E

K

K

Y

W

F

F

G

G

D

E

binding glutamine: E11 (≠ N33), F14 (≠ Y36), F52 (= F74), G70 (≠ S92), M71 (= M93), T72 (= T94), R77 (= R99), S120 (≠ T142), T121 (≠ I143), D159 (= D183)

3vv5A Crystal structure of ttc0807 complexed with (s)-2-aminoethyl-l- cysteine (aec) (see paper)
36% identity, 88% coverage: 27:253/259 of query aligns to 14:232/237 of 3vv5A

query
sites
3vv5A

E

E

L

I

R

R

F

I

G

G

T

T

N
x
E

P

G

S

A

Y
x
F

P

P

F

F

E
x
N

S

Y

T

F

T

D

A

E

D

R

G

N

G

Q

V

L

Q

T

G

G

F

F

G

E

V

V

D

D

L

L

G

G

N

N

A

A

I

I

C

A

A

E

E

R

L

L

K

G

L

L

K

K

C

P

V

R

W

W

V

I

S

A

N

Q

D

S

F
|
F

D

D

G

T

L

L

I

L

P

I

A

Q

L

L

K

N

A

Q

G

G

K

R

F

F

D

D

A

F

I

V

E

I

S
x
A

S
|
S

M

H

T
x
G

V

I

T

T

D

E

A

E

R
|
R

K

A

K

R

Q

A

I

V

D

D

F

F

T

T

D

N

R

P

L

H

Y

Y

A

C

G

T

P

G

T

G

A

V

I

I

V

V

T

S

R

R

K

K

D

-

S

-

G

G

L

G

E

P

P

R

T

T

A

A

E

K

S

D

L

L

K

Q

G

G

K

K

T

V

I

V

G

G

F

V

M

Q

Q

V

G

G

T
|
T

I

-

Q

-

E

-

T
|
T

Y
|
Y

A

M

R

E

-

A

A

A

K

Q

L

K

A

I

P

P

G

G

G

I

V

K

K

E

L

V

R

R

A

T

Y

Y

Q

Q

N

R

Q

D

D

P

Q

D

V

A

Y

L

A

Q

D

D

L

L

V

L

Y

A

G

G

R

R

L

I

D

D

A

T

S

W

I

I

Q

T

D

D

K

R

L

F

Q

V

A

A

Q

K

M

E

S

A

F

I

L

K

E

E

S

R

P

-

Q

-

G

-

A

-

P

K

F

L

K

E

N

N

S

T

Q

L

G

Q

I

V

S

G

D

E

P

L

L

V

V

F

P

Q

A

E

D

R

I

V

A

A

I

M

G

A

V

V

R

A

K

K

D

G

N

N

D

K

E

S

L

L

K

L

G

D

M

A

L

L

N

N

T

R

A

A

I

L

K

A

A

E

L

L

H

M

E

Q

K

D

G

G

I

T

Y

Y

A

A

Q

R

I

I

Q

S

K

Q

K

K

Y

W

F

F

G

G

D

E

binding l-thialysine: E20 (≠ N33), F23 (≠ Y36), N27 (≠ E40), F61 (= F74), A78 (≠ S91), S79 (= S92), G81 (≠ T94), R86 (= R99), T127 (= T142), T128 (= T146), Y129 (= Y147)

3vvfA Crystal structure of ttc0807 complexed with arginine (see paper)
36% identity, 88% coverage: 27:253/259 of query aligns to 18:236/241 of 3vvfA

query
sites
3vvfA

E

E

L

I

R

R

F

I

G

G

T

T

N
x
E

P

G

S

A

Y
x
F

P

P

F

F

E

N

S

Y

T

F

T

D

A

E

D

R

G

N

G

Q

V

L

Q

T

G

G

F

F

G

E

V

V

D

D

L

L

G

G

N

N

A

A

I

I

C

A

A

E

E

R

L

L

K

G

L

L

K

K

C

P

V

R

W

W

V

I

S

A

N

Q

D

S

F
|
F

D

D

G

T

L

L

I

L

P

I

A

Q

L

L

K

N

A

Q

G

G

K

R

F

F

D

D

A

F

I

V

E

I

S
x
A

S
|
S

M
x
H

T
x
G

V

I

T

T

D

E

A

E

R
|
R

K

A

K

R

Q

A

I

V

D

D

F

F

T

T

D

N

R

P

L

H

Y

Y

A

C

G

T

P

G

T

G

A

V

I

I

V

V

T

S

R

R

K

K

D

-

S

-

G

G

L

G

E

P

P

R

T

T

A

A

E

K

S

D

L

L

K

Q

G

G

K

K

T

V

I

V

G

G

F

V

M
x
Q

Q

V

G

G

T
|
T

I

-

Q

-

E

-

T
|
T

Y
|
Y

A

M

R

E

-

A

A

A

K

Q

L

K

A

I

P

P

G

G

G

I

V

K

K

E

L

V

R

R

A

T

Y

Y

Q

Q

N

R

Q

D

D

P

Q

D

V

A

Y

L

A

Q

D

D

L

L

V

L

Y

A

G

G

R

R

L

I

D

D

A

T

S

W

I

I

Q

T

D

D

K

R

L

F

Q

V

A

A

Q

K

M

E

S

A

F

I

L

K

E

E

S

R

P

-

Q

-

G

-

A

-

P

K

F

L

K

E

N

N

S

T

Q

L

G

Q

I

V

S

G

D

E

P

L

L

V

V

F

P

Q

A
x
E

D

R

I

V

A

A

I

M

G

A

V

V

R

A

K

K

D

G

N

N

D

K

E

S

L

L

K

L

G

D

M

A

L

L

N

N

T

R

A

A

I

L

K

A

A

E

L

L

H

M

E

Q

K

D

G

G

I

T

Y

Y

A

A

Q

R

I

I

Q

S

K

Q

K

K

Y

W

F

F

G

G

D

E

binding arginine: E24 (≠ N33), F27 (≠ Y36), F65 (= F74), A82 (≠ S91), S83 (= S92), H84 (≠ M93), G85 (≠ T94), R90 (= R99), Q128 (≠ M139), T131 (= T142), T132 (= T146), Y133 (= Y147), E196 (≠ A213)

3vveA Crystal structure of ttc0807 complexed with lysine (see paper)
36% identity, 88% coverage: 27:253/259 of query aligns to 18:236/241 of 3vveA

query
sites
3vveA

E

E

L

I

R

R

F

I

G

G

T

T

N
x
E

P

G

S

A

Y
x
F

P

P

F

F

E

N

S

Y

T

F

T

D

A

E

D

R

G

N

G

Q

V

L

Q

T

G

G

F

F

G

E

V

V

D

D

L

L

G

G

N

N

A

A

I

I

C

A

A

E

E

R

L

L

K

G

L

L

K

K

C

P

V

R

W

W

V

I

S

A

N

Q

D

S

F
|
F

D

D

G

T

L

L

I

L

P

I

A

Q

L

L

K

N

A

Q

G

G

K

R

F

F

D

D

A

F

I

V

E

I

S

A

S
|
S

M
x
H

T

G

V

I

T

T

D

E

A

E

R
|
R

K

A

K

R

Q

A

I

V

D

D

F

F

T

T

D

N

R

P

L

H

Y

Y

A

C

G

T

P

G

T

G

A

V

I

I

V

V

T

S

R

R

K

K

D

-

S

-

G

G

L

G

E

P

P

R

T

T

A

A

E

K

S

D

L

L

K

Q

G

G

K

K

T

V

I

V

G

G

F

V

M
x
Q

Q

V

G

G

T
|
T

I

-

Q

-

E

-

T
|
T

Y
|
Y

A

M

R

E

-

A

A

A

K

Q

L

K

A

I

P

P

G

G

G

I

V

K

K

E

L

V

R

R

A

T

Y

Y

Q

Q

N

R

Q

D

D

P

Q

D

V

A

Y

L

A

Q

D

D

L

L

V

L

Y

A

G

G

R

R

L

I

D

D

A

T

S

W

I

I

Q

T

D

D

K

R

L

F

Q

V

A

A

Q

K

M

E

S

A

F

I

L

K

E

E

S

R

P

-

Q

-

G

-

A

-

P

K

F

L

K

E

N

N

S

T

Q

L

G

Q

I

V

S

G

D

E

P

L

L

V

V

F

P

Q

A
x
E

D

R

I

V

A

A

I

M

G

A

V

V

R

A

K

K

D

G

N

N

D

K

E

S

L

L

K

L

G

D

M

A

L

L

N

N

T

R

A

A

I

L

K

A

A

E

L

L

H

M

E

Q

K

D

G

G

I

T

Y

Y

A

A

Q

R

I

I

Q

S

K

Q

K

K

Y

W

F

F

G

G

D

E

binding lysine: E24 (≠ N33), F27 (≠ Y36), F65 (= F74), S83 (= S92), H84 (≠ M93), R90 (= R99), Q128 (≠ M139), T131 (= T142), T132 (= T146), Y133 (= Y147), E196 (≠ A213)

3vvdA Crystal structure of ttc0807 complexed with ornithine (see paper)
36% identity, 88% coverage: 27:253/259 of query aligns to 18:236/241 of 3vvdA

query
sites
3vvdA

E

E

L

I

R

R

F

I

G

G

T

T

N
x
E

P

G

S

A

Y

F

P

P

F

F

E

N

S

Y

T

F

T

D

A

E

D

R

G

N

G

Q

V

L

Q

T

G

G

F

F

G

E

V

V

D

D

L

L

G

G

N

N

A

A

I

I

C

A

A

E

E

R

L

L

K

G

L

L

K

K

C

P

V

R

W

W

V

I

S

A

N

Q

D

S

F
|
F

D

D

G

T

L

L

I

L

P

I

A

Q

L

L

K

N

A

Q

G

G

K

R

F

F

D

D

A

F

I

V

E

I

S

A

S
|
S

M
x
H

T
x
G

V

I

T

T

D

E

A

E

R
|
R

K

A

K

R

Q

A

I

V

D

D

F

F

T

T

D

N

R

P

L

H

Y

Y

A

C

G

T

P

G

T

G

A

V

I

I

V

V

T

S

R

R

K

K

D

-

S

-

G

G

L

G

E

P

P

R

T

T

A

A

E

K

S

D

L

L

K

Q

G

G

K

K

T

V

I

V

G

G

F

V

M
x
Q

Q

V

G

G

T
|
T

I

-

Q

-

E

-

T
|
T

Y
|
Y

A

M

R

E

-

A

A

A

K

Q

L

K

A

I

P

P

G

G

G

I

V

K

K

E

L

V

R

R

A

T

Y

Y

Q

Q

N

R

Q

D

D

P

Q

D

V

A

Y

L

A

Q

D

D

L

L

V

L

Y

A

G

G

R

R

L

I

D

D

A

T

S

W

I

I

Q

T

D

D

K

R

L

F

Q

V

A

A

Q

K

M

E

S

A

F

I

L

K

E

E

S

R

P

-

Q

-

G

-

A

-

P

K

F

L

K

E

N

N

S

T

Q

L

G

Q

I

V

S

G

D

E

P

L

L

V

V

F

P

Q

A
x
E

D

R

I

V

A

A

I

M

G

A

V

V

R

A

K

K

D

G

N

N

D

K

E

S

L

L

K

L

G

D

M

A

L

L

N

N

T

R

A

A

I

L

K

A

A

E

L

L

H

M

E

Q

K

D

G

G

I

T

Y

Y

A

A

Q

R

I

I

Q

S

K

Q

K

K

Y

W

F

F

G

G

D

E

binding L-ornithine: E24 (≠ N33), F65 (= F74), S83 (= S92), H84 (≠ M93), G85 (≠ T94), R90 (= R99), Q128 (≠ M139), T131 (= T142), T132 (= T146), Y133 (= Y147), E196 (≠ A213)

2y7iA Structural basis for high arginine specificity in salmonella typhimurium periplasmic binding protein stm4351. (see paper)
35% identity, 86% coverage: 28:251/259 of query aligns to 8:227/228 of 2y7iA

query
sites
2y7iA

L

L

R
x
H

F

F

G

G

T

T

N

S

P

A

S

T

Y
|
Y

P

A

P

P

F

Y

E
|
E

S

F

T

V

T
x
D

A

A

D
|
D

G

N

G

K

V

I

Q

V

G

G

F

F

G

D

V

I

D

D

L

V

G

A

N

N

A

A

I

V

C

C

A

K

E

E

L

M

K

Q

L

A

K

E

C

C

V

S

W

F

V

T

S

N

N

Q

D

S

F
|
F

D
|
D

G

S

L

L

I

I

P

P

A

S

L

L

K

R

A

F

G

K

K

K

F

F

D
|
D

A

A

I

V

E

I

S
x
A

S
x
G

M

M

T
x
D

V

M

T

T

D

P

A

K

R
|
R

K

E

K

Q

Q

Q

I

V

D

S

F

F

T

S

D

Q

R

P

L

Y

Y

Y

A

E

G

G

P

L

T

S

A

A

-

V

I

V

V

V

T

T

R

R

K

K

D

-

S

-

G

G

L

A

E

Y

P

H

T

T

A

F

E

A

S

D

L

L

K

K

G

G

K

K

T

K

I

V

G

G

F

L

M
x
E

Q

N

G

G

T
|
T

I
x
T

Q

H

E

Q

T

R

Y

Y

A

L

R

Q

A

D

K

K

L

-

A

-

P

Q

G

Q

G

A

V

I

K

T

L

P

R

V

A

A

Y

Y

Q
x
D

N

S

Q

Y

D

L

Q

N

V

A

Y

F

A

T

D

D

L

L

V

K

Y

N

G

N

R

R

L

L

D

E

A

G

S

V

I

F

Q

G

D
|
D

K

V

L

A

Q

A

A

I

Q

G

M

K

S

W

F

L

L

K

E

N

S

N

P

P

Q

D

G

Y

A

A

P

I

F

M

K

-

N

-

S

D

Q

E

G

R

I

A

S

S

D

D

P

P

-

D

L

Y

V

Y

P

G

A

K

D

G

I

L

A

G

I

I

G

A

V

V

R

R

K

K

D

D

N

N

D

D

E

A

L

L

K

L

G

Q

M

E

L

I

N

N

T

A

A

A

I

L

K

D

A

K

L

V

H

K

E

A

K

S

G

P

I

E

Y

Y

A

A

Q

Q

I

M

Q

Q

K

E

K

K

Y

W

F

F

binding arginine: Y16 (= Y36), E20 (= E40), F54 (= F74), A71 (≠ S91), G72 (≠ S92), D74 (≠ T94), R79 (= R99), E118 (≠ M139), T121 (= T142), T122 (≠ I143), D160 (= D183)
binding zinc ion: H9 (≠ R29), D23 (≠ T43), D25 (= D45), D55 (= D75), D67 (= D87), D140 (≠ Q163)

3tqlA Structure of the amino acid abc transporter, periplasmic amino acid- binding protein from coxiella burnetii. (see paper)
32% identity, 86% coverage: 28:251/259 of query aligns to 4:225/225 of 3tqlA

query
sites
3tqlA

L

I

R

K

F

F

G

A

T

T

N
x
E

P

A

S

T

Y
|
Y

P

P

F

Y

E

-

S

-

T

V

T

Y

A

M

D

G

G

G

G

Q

V

V

Q

E

G

G

F

F

G

G

V

A

D

D

L

I

G

V

N

K

A

A

I

V

C

C

A

K

E

Q

L

M

K

Q

L

A

K

V

C

C

V

T

W

I

V

S

S

N

N

Q

D

P

F
x
W

D

D

G

S

L

L

I

I

P

P

A

S

L

L

K

K

A

L

G

G

K

K

F

F

D

D

A

A

I

L

E

F

S
x
G

M

M

T
x
N

V

I

T

T

D

T

A

A

R
|
R

K

Q

K

K

Q

E

I

V

D

D

F

F

T

T

D

D

R

P

L

Y

Y

Y

A

T

G

N

P

S

T

V

A
x
S

I

F

V

I

T

A

R

D

K

K

D

N

S

T

G

P

L

L

E

T

P

L

T

S

A

K

E

Q

S

G

L

L

K

K

G

G

K

K

T

I

I

I

G

G

F

V

M

Q

Q

G

G

G

T

T

I

T

Q

F

E

D

T

S

Y

Y

A

L

R

Q

A

D

K

S

L

F

A

G

P

-

G

N

G

S

V

I

K

T

L

I

R

Q

A

R

Y

Y

Q

P

N

S

Q

E

D

E

Q

D

V

A

Y

L

A

M

D

D

L

L

V

T

Y

S

G

G

R

R

L

V

D

D

A

A

S

V

I

V

Q

G

D

D

K

T

L

P

Q

L

A

I

Q

K

M

Q

S

W

F

L

L

K

E

Q

S

N

P

G

Q

R

G

R

A

E

P

Y

F

V

K

L

N

I

S

G

Q

K

G

P

I

V

S

N

D

D

P

P

-

N

L

Y

V

F

P

G

A

K

D

G

I

V

A

G

I

I

G

A

V

V

R

K

K

K

D

G

N

N

D

Q

E

A

L

L

K

L

G

L

M

K

L

L

N

N

T

K

A

A

I

L

K

A

A

A

L

I

H

K

E

A

K

N

G

G

I

V

Y

Y

A

A

Q

A

I

I

Q

V

K

Q

K

K

Y

Y

F

F

binding arginine: E9 (≠ N33), Y12 (= Y36), W48 (≠ F74), G65 (≠ S91), G66 (≠ S92), N68 (≠ T94), R73 (= R99), S89 (≠ A115)

5t0wA Crystal structure of the ancestral amino acid-binding protein anccdt- 1, a precursor of cyclohexadienyl dehydratase
31% identity, 86% coverage: 28:251/259 of query aligns to 12:228/229 of 5t0wA

query
sites
5t0wA

L

L

R

R

F

V

G

G

T

T

N
x
D

P

A

S

D

Y
|
Y

P

K

P

P

F

F

E

S

S

F

T

K

T

D

A

K

D

N

G

G

G

Q

V

Y

Q

T

G

G

F

F

G

D

V

I

D

D

L

L

G

A

N

K

A

A

I

L

C

A

A

K

E

E

L

L

K

G

L

V

K

K

C

V

V

E

W

F

V

V

S

P

N

T

D

T

F
x
W

D

D

G

G

L

I

I

I

P

P

A

A

L

L

K

Q

A

T

G

G

K

K

F

F

D

D

A

I

I

V

E

M

S
|
S

S
x
G

M
|
M

T
|
T

V

I

T

T

D

P

A

E

R
|
R

K

K

Q

K

I

V

D

D

F

F

T

S

D

D

-

P

R

Y

L

M

Y

T

A

A

G

G

P

Q

T

T

A

I

I

L

V

V

T

K

R

K

K

D

D

N

S

A

G

D

L

K

E

I

P

K

T

S

A

F

E

E

S

D

L

L

K

N

G

K

K

P

-

D

-

V

T

K

I

V

G

A

F

V

M
x
Q

Q

L

G

G

T
|
T

I
x
T

Q

S

E

E

T

Q

Y

A

A

A

R

K

A

E

K

F

L

L

A

P

P

K

G

A

G

-

V

-

K

K

L

I

R

R

A

T

Y

F

Q

E

N

N

Q

N

D

A

Q

E

V

A

Y

F

A

Q

D

E

L

V

V

V

Y

S

G

G

R

R

L

A

D

D

A

A

S

M

I

V

Q

T

D
|
D

K

-

L

-

Q

-

A

-

Q

-

M

-

S

-

F

-

L

-

E

-

S

S

P

P

Q

V

G

A

A

A

P

Y

F

Y

K

A

N

K

-

L

-

A

S

V

Q

V

G

V

I

V

S

D

D

E

P

P

L

F

V

T

P

H

A

E

D

P

I

L

A

G

I

F

G

A

V

I

R

R

K

K

D

G

N

D

D

P

E

E

L

L

K

L

G

N

M

W

L

V

N

N

T

N

A

W

I

L

K

K

A

Q

L

M

H

K

E

K

K

D

G

G

I

T

Y

Y

A

D

Q

K

I

L

Q

Y

K

E

K

K

Y

W

F

F

binding arginine: D17 (≠ N33), Y20 (= Y36), W58 (≠ F74), S75 (= S91), G76 (≠ S92), M77 (= M93), T78 (= T94), R83 (= R99), Q126 (≠ M139), T129 (= T142), T130 (≠ I143), D168 (= D183)

8eyzA Engineered glutamine binding protein bound to gln and a cobaloxime ligand (see paper)
34% identity, 87% coverage: 27:252/259 of query aligns to 4:222/226 of 8eyzA

query
sites
8eyzA

E

K

L

L

R

V

F

V

G

A

T

T

N
x
D

P

T

S

A

Y
x
F

P

V

P

P

F

F

E

E

S

F

T

K

T

Q

A

G

D

D

G

I

G

Y

V

V

Q

-

G

G

F

F

G

D

V

V

D

D

L

L

G

W

N

A

A

A

I

I

C

A

A

K

E

E

L

L

K

K

L

L

K

D

C

Y

V

E

W

L

V

K

S

P

N

M

D

D

F
|
F

D

S

G

G

L

I

I

I

P

P

A

A

L

L

K

Q

A

T

G

K

K

N

F

V

D

D

A

L

I

A

E

L

S
x
A

S
x
G

M

I

T
|
T

V

I

T
x
C

D

D

A

E

R
|
R

K

K

Q

A

I

I

D

D

F

F

T

S

D

D

R

G

L

Y

Y

Y

A

K

G

S

P

G

T

L

A

L

I

V

V

M

T

V

R

K

K

A

D

N

S

N

G

N

L

D

E

V

P

K

T

S

A

V

E

K

S

D

L

L

K

D

G

G

K

K

T

V

I

V

G

A

F

V

M

K

Q

S

G

G

T

T

I
x
G

Q

S

E

V

T
x
D

Y

Y

A

A

R

K

A

A

K

N

L

I

A

K

P

T

G

K

G

-

V

-

K

D

L

L

R

R

A

Q

Y

F

Q

P

N

N

Q

I

D

D

Q

N

V

A

Y

Y

A

M

D

E

L

L

V

G

Y

T

G

N

R

R

L

A

D

D

A

A

S

V

I

L

Q

H

D
|
D

K

T

L

P

Q

N

A

I

Q

-

M

L

S

Y

F

F

L

I

E

K

S

T

P

A

Q

G

G

N

A

G

P

Q

F

F

K

K

N

-

S

-

Q

-

G

A

I

V

S

G

D

D

P

S

L

L

V

E

P

A

A

Q

D

Q

I

Y

A

G

I

I

G

A

V

F

R

P

K

K

D

G

N

S

D

D

E

E

L

L

K

R

G

D

M

K

L

V

N

N

T

G

A

A

I

L

K

K

A

T

L

L

H

R

E

E

K

N

G

G

I

T

Y

Y

A

N

Q

E

I

I

Q

Y

K

K

Y

W

F

F

G

G

binding glutamine: D10 (≠ N33), F13 (≠ Y36), F50 (= F74), A67 (≠ S91), G68 (≠ S92), T70 (= T94), R75 (= R99), D157 (= D183)
binding azidobis (dimethylglyoximato) pyridinecobalt: C72 (≠ T96), G119 (≠ I143), D122 (≠ T146)

6svfA Crystal structure of the p235gk mutant of argbp from t. Maritima (see paper)
32% identity, 87% coverage: 28:253/259 of query aligns to 12:229/229 of 6svfA

query
sites
6svfA

L

L

R

L

F

V

G

G

T

L

N

S

P

A

S

D

Y
x
F

P

P

F

F

E

E

S

F

T

V

T

D

A

E

D

N

G

G

G

N

V

I

Q

V

G

G

F

F

G

D

V

V

D

D

L

L

G

A

N

K

A

E

I

I

C

A

A

R

E

R

L

L

K

G

L

V

K

E

C

L

V

K

W

I

V

V

S

D

N

M

D

T

F
|
F

D

D

G

G

L

L

I

I

P

P

A

S

L

L

K

L

A

T

G

K

K

K

F

I

D

D

A

V

I

I

E

I

S
|
S

S
x
G

M
|
M

T
|
T

V

I

T

T

D

E

A

E

R
|
R

K

K

Q

V

I

V

D

A

F

F

T

S

D

D

R

P

L

Y

Y

F

A

D

G

A

P

G

T

Q

A

V

I

I

V

V

T

V

R

R

K

K

D

D

S

S

G

D

L

F

E

R

P

P

-

K

T

T

A

Y

E

E

S

D

L

L

K

V

G

G

K

K

T

T

I

V

G

A

F

V

M
x
Q

Q

I

G

G

T
|
T

I
x
T

Q

G

E

D

T

I

Y

E

A

V

R

S

A

K

K

Y

L

-

A

-

P

-

G

D

G

G

V

I

K

K

L

V

R

V

A

R

Y

F

Q

D

N

K

Q

F

D

T

Q

D

V

A

Y

F

A

L

D

E

L

L

V

K

Y

R

G

G

R

R

L

A

D

D

A

A

S

V

I

V

Q

L

D
|
D

K

S

L

A

Q

T

A

A

Q

R

M

A

S

F

F

V

L

A

E

K

S

N

P

P

Q

D

G

-

A

-

P

-

F

L

K

V

N

I

S

S

Q

S

G

G

I

V

S

-

D

-

P

-

L

L

V

S

P

S

A

E

D

Q

I

Y

A

G

I

I

G

A

V

V

R

R

K

K

D

E

N

D

D

T

E

D

L

L

K

L

G

E

M

F

L

I

N

N

T

S

A

V

I

L

K

R

A

E

L

L

H

K

E

K

K

S

G

G

I

K

Y

Y

A

D

Q

V

I

L

Q

I

K

E

K

K

Y

W

F

F

G

S

D

E

binding arginine: F20 (≠ Y36), F58 (= F74), S75 (= S91), G76 (≠ S92), M77 (= M93), T78 (= T94), R83 (= R99), Q124 (≠ M139), T127 (= T142), T128 (≠ I143), D165 (= D183)

Query Sequence

>AO353_25660 FitnessBrowser__pseudo3_N2E3:AO353_25660
MKTQWLTLPVLAMLFCSAGASAKEWTELRFGTNPSYPPFESTTADGGVQGFGVDLGNAIC
AELKLKCVWVSNDFDGLIPALKAGKFDAIESSMTVTDARKKQIDFTDRLYAGPTAIVTRK
DSGLEPTAESLKGKTIGFMQGTIQETYARAKLAPGGVKLRAYQNQDQVYADLVYGRLDAS
IQDKLQAQMSFLESPQGAPFKNSQGISDPLVPADIAIGVRKDNDELKGMLNTAIKALHEK
GIYAQIQKKYFGDLDLYNN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory