SitesBLAST
Comparing AO353_26885 FitnessBrowser__pseudo3_N2E3:AO353_26885 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
51% identity, 82% coverage: 53:322/328 of query aligns to 54:325/334 of 5aovA
- active site: L100 (= L99), R241 (= R237), D265 (= D261), E270 (= E266), H288 (= H285)
- binding glyoxylic acid: Y74 (≠ V73), A75 (≠ S74), V76 (= V75), G77 (= G76), R241 (= R237), H288 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V75), T104 (= T103), F158 (≠ M153), G159 (= G154), R160 (= R155), I161 (= I156), S180 (= S176), R181 (≠ H177), A211 (≠ T207), V212 (≠ L208), P213 (= P209), T218 (= T214), I239 (= I235), A240 (≠ S236), R241 (= R237), H288 (= H285), G290 (= G287)
Sites not aligning to the query:
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
51% identity, 82% coverage: 53:322/328 of query aligns to 53:324/332 of 6biiA
- active site: L99 (= L99), R240 (= R237), D264 (= D261), E269 (= E266), H287 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (= V75), T103 (= T103), G156 (= G152), F157 (≠ M153), G158 (= G154), R159 (= R155), I160 (= I156), A179 (≠ S176), R180 (≠ H177), S181 (= S178), K183 (= K180), V211 (≠ L208), P212 (= P209), E216 (= E213), T217 (= T214), V238 (≠ I235), A239 (≠ S236), R240 (= R237), D264 (= D261), H287 (= H285), G289 (= G287)
O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
50% identity, 82% coverage: 53:322/328 of query aligns to 54:325/334 of O58320
2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
50% identity, 82% coverage: 53:322/328 of query aligns to 54:325/333 of 2dbqA
- active site: L100 (= L99), R241 (= R237), D265 (= D261), E270 (= E266), H288 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V75), T104 (= T103), L158 (≠ M153), G159 (= G154), R160 (= R155), I161 (= I156), S180 (= S176), R181 (≠ H177), T182 (≠ S178), A211 (≠ T207), V212 (≠ L208), P213 (= P209), T218 (= T214), I239 (= I235), A240 (≠ S236), R241 (= R237), D265 (= D261), H288 (= H285), G290 (= G287)
2gcgA Ternary crystal structure of human glyoxylate reductase/hydroxypyruvate reductase (see paper)
43% identity, 88% coverage: 23:312/328 of query aligns to 25:316/324 of 2gcgA
- active site: L103 (= L99), R241 (= R237), D265 (= D261), E270 (= E266), H289 (= H285)
- binding (2r)-2,3-dihydroxypropanoic acid: L55 (vs. gap), S78 (= S74), V79 (= V75), G80 (= G76), R241 (= R237), H289 (= H285)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V79 (= V75), T107 (= T103), G156 (= G152), G158 (= G154), I160 (= I156), G180 (≠ S176), R181 (≠ H177), R184 (≠ K180), C212 (≠ L208), S213 (≠ P209), T218 (= T214), I239 (= I235), R241 (= R237), D265 (= D261), H289 (= H285), G291 (= G287)
Q9UBQ7 Glyoxylate reductase/hydroxypyruvate reductase; EC 1.1.1.79; EC 1.1.1.81 from Homo sapiens (Human) (see paper)
43% identity, 88% coverage: 23:312/328 of query aligns to 29:320/328 of Q9UBQ7
Q65CJ7 Hydroxyphenylpyruvate reductase; HPPR; EC 1.1.1.237 from Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides) (see paper)
43% identity, 78% coverage: 57:312/328 of query aligns to 58:306/313 of Q65CJ7
3bazA Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+ (see paper)
43% identity, 78% coverage: 57:312/328 of query aligns to 56:304/311 of 3bazA
- active site: L98 (= L99), R230 (= R237), A251 (= A258), D254 (= D261), E259 (= E266), H277 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V74 (= V75), G149 (= G152), L150 (≠ M153), G151 (= G154), R152 (= R155), I153 (= I156), S172 (= S176), R173 (≠ H177), S174 (= S178), C201 (≠ L208), P202 (= P209), T207 (= T214), I228 (= I235), G229 (≠ S236), R230 (= R237), D254 (= D261), H277 (= H285), G279 (= G287)
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
39% identity, 96% coverage: 5:319/328 of query aligns to 4:311/526 of 3dc2A
Sites not aligning to the query:
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
39% identity, 96% coverage: 5:319/328 of query aligns to 5:312/525 of 3ddnB
4e5mA Thermostable phosphite dehydrogenase e175a/a176r in complex with NADP (see paper)
40% identity, 81% coverage: 55:319/328 of query aligns to 56:326/329 of 4e5mA
- active site: L100 (= L99), R237 (= R237), D261 (= D261), E266 (= E266), H292 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: K76 (≠ V75), L100 (= L99), T104 (= T103), G154 (= G154), A155 (≠ R155), I156 (= I156), R176 (≠ H177), L208 (= L208), P209 (= P209), T214 (= T214), P235 (≠ I235), C236 (≠ S236), R237 (= R237), H292 (= H285), G294 (= G287)
4e5pA Thermostable phosphite dehydrogenase a176r variant in complex with nad (see paper)
40% identity, 81% coverage: 55:319/328 of query aligns to 56:326/332 of 4e5pA
- active site: L100 (= L99), R237 (= R237), D261 (= D261), E266 (= E266), H292 (= H285)
- binding nicotinamide-adenine-dinucleotide: K76 (≠ V75), L100 (= L99), T104 (= T103), G154 (= G154), A155 (≠ R155), I156 (= I156), A175 (≠ S176), R176 (≠ H177), L208 (= L208), P209 (= P209), T214 (= T214), P235 (≠ I235), C236 (≠ S236), R237 (= R237), H292 (= H285)
4e5kA Thermostable phosphite dehydrogenase in complex with NAD and sulfite (see paper)
39% identity, 81% coverage: 55:319/328 of query aligns to 56:326/329 of 4e5kA
- active site: L100 (= L99), R237 (= R237), D261 (= D261), E266 (= E266), H292 (= H285)
- binding nicotinamide-adenine-dinucleotide: K76 (≠ V75), G77 (= G76), L100 (= L99), T104 (= T103), G152 (= G152), G154 (= G154), A155 (≠ R155), I156 (= I156), H174 (= H175), E175 (vs. gap), A176 (≠ S176), A207 (≠ T207), L208 (= L208), P209 (= P209), P235 (≠ I235), C236 (≠ S236), R237 (= R237), D261 (= D261), H292 (= H285), G294 (= G287)
- binding sulfite ion: L75 (≠ S74), K76 (≠ V75), G77 (= G76), L100 (= L99), R237 (= R237), H292 (= H285)
Sites not aligning to the query:
5j23A Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with 2'-phospho-adp-ribose (see paper)
35% identity, 93% coverage: 2:307/328 of query aligns to 1:297/318 of 5j23A
- active site: L94 (= L99), R228 (= R237), D252 (= D261), E257 (= E266), H275 (= H285)
- binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4r,5r)-3,4,5-trihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: V70 (= V75), L148 (≠ M153), G149 (= G154), R150 (= R155), I151 (= I156), T170 (≠ S176), R171 (≠ H177), P200 (= P209), S204 (≠ E213), T205 (= T214), R228 (= R237)
5v7nA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and 2-keto-d-gluconic acid (see paper)
35% identity, 93% coverage: 2:307/328 of query aligns to 2:298/319 of 5v7nA
- active site: L95 (= L99), R229 (= R237), D253 (= D261), E258 (= E266), H276 (= H285)
- binding 2-keto-D-gluconic acid: G70 (≠ S74), V71 (= V75), G72 (= G76), R229 (= R237), H276 (= H285), S279 (= S288), R285 (= R294)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V71 (= V75), V99 (≠ T103), L149 (≠ M153), G150 (= G154), R151 (= R155), I152 (= I156), T171 (≠ S176), R172 (≠ H177), V200 (≠ L208), P201 (= P209), S205 (≠ E213), T206 (= T214), V227 (≠ I235), G228 (≠ S236), R229 (= R237), H276 (= H285), A278 (≠ G287)
5v6qB Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and malonate (see paper)
35% identity, 93% coverage: 2:307/328 of query aligns to 3:299/319 of 5v6qB
- active site: L96 (= L99), R230 (= R237), D254 (= D261), E259 (= E266), H277 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V72 (= V75), V100 (≠ T103), F148 (≠ I151), L150 (≠ M153), G151 (= G154), R152 (= R155), I153 (= I156), T172 (≠ S176), R173 (≠ H177), V201 (≠ L208), P202 (= P209), S206 (≠ E213), T207 (= T214), V228 (≠ I235), G229 (≠ S236), R230 (= R237), H277 (= H285), A279 (≠ G287)
5v7gA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADPH and oxalate (see paper)
35% identity, 93% coverage: 2:307/328 of query aligns to 1:297/317 of 5v7gA
- active site: L94 (= L99), R228 (= R237), D252 (= D261), E257 (= E266), H275 (= H285)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V70 (= V75), V98 (≠ T103), F146 (≠ I151), L148 (≠ M153), G149 (= G154), R150 (= R155), I151 (= I156), T170 (≠ S176), R171 (≠ H177), V199 (≠ L208), P200 (= P209), S204 (≠ E213), T205 (= T214), V226 (≠ I235), G227 (≠ S236), R228 (= R237), H275 (= H285), A277 (≠ G287)
- binding oxalate ion: G69 (≠ S74), V70 (= V75), G71 (= G76), R228 (= R237), H275 (= H285)
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
33% identity, 82% coverage: 52:321/328 of query aligns to 55:319/533 of O43175
- T78 (≠ V75) binding
- R135 (= R132) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (= RI 155:156) binding
- D175 (≠ S176) binding
- T207 (≠ L208) binding
- CAR 234:236 (≠ ISR 235:237) binding
- D260 (= D261) binding
- V261 (= V262) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HIGS 285:288) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
34% identity, 79% coverage: 43:301/328 of query aligns to 40:291/303 of 6plgA
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
34% identity, 76% coverage: 52:301/328 of query aligns to 50:291/301 of 6rj5A
Query Sequence
>AO353_26885 FitnessBrowser__pseudo3_N2E3:AO353_26885
MKKQVVLYKKLSAPLMARLHEQAEVTLIDSLDAEGLKRLREALPSAHGLLGASLKLDAEL
LDLAPRLEAIASVSVGVDNYDIDYLNRRQILLSNTPDVLTETTADTGFALILATARRVVE
LANLVRAGQWNRNIGPAHFGTDVHGKTLGIIGMGRIGEALAQRGHFGFGMPILYHSHSVK
PAVEQRFNAQYRSLPALLQQADFICLTLPLTAETEGLIGAEQFALMRPESIFINISRGKV
VDETALIEALRQGQIRAAGLDVFEREPLAYDSPLLQLNNVVATPHIGSATHETREAMARC
AVDNLLAALAGERPANLVNGQVWETQNL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory