SitesBLAST
Comparing AO353_26925 FitnessBrowser__pseudo3_N2E3:AO353_26925 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1cjxA Crystal structure of pseudomonas fluorescens hppd (see paper)
93% identity, 98% coverage: 5:356/358 of query aligns to 1:352/352 of 1cjxA
7xntA Crystal structure of pfhppd-y13161 complex
92% identity, 97% coverage: 2:349/358 of query aligns to 1:341/341 of 7xntA
- binding 3-(2,6-dimethylphenyl)-1-methyl-6-(2-oxidanyl-6-oxidanylidene-cyclohexen-1-yl)carbonyl-quinazoline-2,4-dione: H161 (= H162), T163 (= T164), S201 (= S202), P214 (= P215), Q221 (= Q226), H236 (= H241), F304 (= F312), F313 (= F321), F325 (= F333), F330 (= F338)
- binding cobalt (ii) ion: H161 (= H162), H236 (= H241), E315 (= E323)
7x8eA Crystal structure of pfhppd-y13287 complex
90% identity, 98% coverage: 3:353/358 of query aligns to 1:341/341 of 7x8eA
- binding 1,5-dimethyl-3-(2-methylphenyl)-6-(2-oxidanyl-6-oxidanylidene-cyclohexen-1-yl)carbonyl-quinazoline-2,4-dione: S193 (= S202), P206 (= P215), Q214 (= Q226), H229 (= H241), M252 (= M264), F300 (= F312), F321 (= F333), F326 (= F338)
- binding cobalt (ii) ion: H160 (= H162), H229 (= H241), E311 (= E323)
7xntC Crystal structure of pfhppd-y13161 complex
87% identity, 96% coverage: 3:344/358 of query aligns to 1:320/320 of 7xntC
- binding 3-(2,6-dimethylphenyl)-1-methyl-6-(2-oxidanyl-6-oxidanylidene-cyclohexen-1-yl)carbonyl-quinazoline-2,4-dione: H160 (= H162), T162 (= T164), P201 (= P215), Q206 (= Q226), H221 (= H241), M244 (= M264), F288 (= F312), F309 (= F333), G310 (= G334), N313 (= N337), F314 (= F338)
- binding cobalt (ii) ion: H160 (= H162), H221 (= H241), E299 (= E323)
Q88JU3 3-dehydroshikimate dehydratase; DSD; EC 4.2.1.118 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
33% identity, 94% coverage: 13:347/358 of query aligns to 295:624/635 of Q88JU3
- H443 (= H162) binding
- H521 (= H241) binding
- E599 (= E323) binding
Sites not aligning to the query:
- 134 binding
- 165 binding
- 168 H→A: 21-fold decrease in turnover rate.
- 191 binding
- 206 S→A: 10-fold decrease in the affinity for dehydroshikimate without significantly altering the turnover rate.
- 239 binding
5hmqD Xylose isomerase-like tim barrel/4-hydroxyphenylpyruvate dioxygenase fusion protein
33% identity, 94% coverage: 13:347/358 of query aligns to 297:621/624 of 5hmqD
Sites not aligning to the query:
1t47A Structure of fe2-hppd bound to ntbc (see paper)
35% identity, 78% coverage: 72:349/358 of query aligns to 79:360/362 of 1t47A
- binding fe (ii) ion: H172 (= H162), H255 (= H241), E334 (= E323)
- binding 2-{hydroxy[2-nitro-4-(trifluoromethyl)phenyl]methylene}cyclohexane-1,3-dione: H172 (= H162), S215 (= S202), P228 (= P215), H255 (= H241), F321 (= F312), E334 (= E323), F344 (= F333), G345 (= G334), N348 (= N337), F349 (= F338), L352 (= L341)
8jbwA Crystal structure of zthppd-(+)-usnic acid complex (see paper)
32% identity, 78% coverage: 73:352/358 of query aligns to 91:364/364 of 8jbwA
- binding (9bR)-2,6-diethanoyl-8,9b-dimethyl-3,7,9-tris(oxidanyl)dibenzofuran-1-one: M196 (= M172), I220 (≠ E196), F224 (≠ L200), H252 (= H241)
- binding cobalt (ii) ion: H186 (= H162), H252 (= H241), Q320 (= Q310), E335 (= E323)
O52791 4-hydroxymandelate synthase; HMS; HmaS; 4-hydroxyphenylpyruvate dioxygenase II; EC 1.13.11.46 from Amycolatopsis orientalis (Nocardia orientalis) (see paper)
30% identity, 88% coverage: 31:346/358 of query aligns to 29:343/357 of O52791
- S201 (= S202) binding
- T214 (≠ P215) binding
- H241 (= H241) binding
- Q305 (= Q310) binding
2r5vA Hydroxymandelate synthase crystal structure (see paper)
30% identity, 88% coverage: 31:346/358 of query aligns to 28:341/346 of 2r5vA
- binding cobalt (ii) ion: H159 (= H162), H239 (= H241), E318 (= E323)
- binding (2S)-hydroxy(4-hydroxyphenyl)ethanoic acid: H159 (= H162), F186 (≠ R189), S199 (= S202), V201 (≠ A204), T212 (≠ P215), H239 (= H241), Q303 (= Q310), E318 (= E323), F328 (= F333), I333 (≠ F338)
P93836 4-hydroxyphenylpyruvate dioxygenase; 4-hydroxyphenylpyruvic acid oxidase; 4HPPD; HPD; HPPDase; EC 1.13.11.27 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 79% coverage: 64:346/358 of query aligns to 125:432/445 of P93836
- H226 (= H162) binding
- H308 (= H241) binding
- E394 (= E323) binding
7x8iA Crystal structure of athppd-shikonin complex (see paper)
29% identity, 79% coverage: 64:346/358 of query aligns to 91:376/380 of 7x8iA
- binding cobalt (ii) ion: H186 (= H162), H259 (= H241), E345 (= E323)
- binding 2-[(1R)-4-methyl-1-oxidanyl-pent-3-enyl]-5,8-bis(oxidanyl)naphthalene-1,4-dione: L216 (= L200), Q244 (= Q226), H259 (= H241), F332 (= F312), F343 (= F321), F363 (vs. gap), F368 (= F338)
7x64A Crystal structure of athppd-y18027 complex (see paper)
29% identity, 79% coverage: 64:346/358 of query aligns to 91:376/380 of 7x64A
- binding 1,5-dimethyl-6-(2-oxidanyl-6-oxidanylidene-cyclohexen-1-yl)carbonyl-3-(phenylmethyl)quinazoline-2,4-dione: H186 (= H162), S218 (= S202), P231 (= P215), R241 (≠ G223), Q244 (= Q226), H259 (= H241), F332 (= F312), F363 (vs. gap), G364 (vs. gap), N367 (= N337), F368 (= F338)
- binding cobalt (ii) ion: H186 (= H162), H259 (= H241), E345 (= E323)
7x5sA Crystal structure of athppd-y14157 complex
29% identity, 79% coverage: 64:346/358 of query aligns to 91:376/380 of 7x5sA
- binding 2-methyl-4-(2-nitro-4-piperidin-1-yl-phenyl)carbonyl-5-phenyl-1H-pyrazol-3-one: H186 (= H162), P231 (= P215), H259 (= H241), F332 (= F312), F343 (= F321), F363 (vs. gap), K365 (vs. gap), N367 (= N337), F368 (= F338)
- binding cobalt (ii) ion: H186 (= H162), H259 (= H241), E345 (= E323)
7ezqA Complex structure of athppd with inhibitor y15832
29% identity, 79% coverage: 64:346/358 of query aligns to 91:376/382 of 7ezqA
- binding 6-(1,3-dimethyl-5-oxidanyl-pyrazol-4-yl)carbonyl-1,5-dimethyl-3-(2-methylphenyl)quinazoline-2,4-dione: H186 (= H162), P231 (= P215), Q244 (= Q226), H259 (= H241), F332 (= F312), E345 (= E323), F363 (vs. gap), G364 (vs. gap), N367 (= N337), F368 (= F338), L371 (= L341)
- binding cobalt (ii) ion: H186 (= H162), H259 (= H241), E345 (= E323)
6isdA Crystal structure of arabidopsis thaliana hppd complexed with sulcotrione (see paper)
29% identity, 79% coverage: 64:346/358 of query aligns to 89:367/369 of 6isdA
- binding 2-[2-chloro-4-(methylsulfonyl)benzoyl]cyclohexane-1,3-dione: H179 (= H162), V181 (≠ T164), S211 (= S202), P224 (= P215), H250 (= H241), F323 (= F312), F334 (= F321), F354 (vs. gap), F359 (= F338)
- binding cobalt (ii) ion: H179 (= H162), H250 (= H241), E336 (= E323)
8iokA Crystal structure of athppd-clj507 complex
30% identity, 79% coverage: 64:346/358 of query aligns to 91:376/379 of 8iokA
- binding cobalt (ii) ion: H186 (= H162), H260 (= H241), E346 (= E323)
- binding 6-[diethylcarbamoyl(methyl)amino]-N-(1-methyl-1,2,3,4-tetrazol-5-yl)-2-(trifluoromethyl)pyridine-3-carboxamide: H186 (= H162), P232 (= P215), H260 (= H241), F333 (= F312), F344 (= F321), E346 (= E323), F363 (= F333), F368 (= F338)
8wqmA Complex structure of athppd with atovaquone
29% identity, 79% coverage: 64:346/358 of query aligns to 91:377/383 of 8wqmA
- binding 2-[trans-4-(4-chlorophenyl)cyclohexyl]-3-hydroxynaphthalene-1,4-dione: H186 (= H162), S219 (= S202), H260 (= H241), F333 (= F312), F364 (vs. gap), G365 (vs. gap), N368 (= N337), F369 (= F338)
- binding cobalt (ii) ion: H186 (= H162), H260 (= H241), E346 (= E323)
7x5rA Crystal structure of athppd-lancotrione complex
29% identity, 79% coverage: 64:346/358 of query aligns to 91:377/386 of 7x5rA
- binding (1R,3R)-2-[(S)-[2-chloranyl-3-[2-(1,3-dioxolan-2-yl)ethoxy]-4-methylsulfonyl-phenyl]-oxidanyl-methyl]cyclohexane-1,3-diol: H186 (= H162), S219 (= S202), P232 (= P215), H260 (= H241), M287 (= M264), F333 (= F312), F344 (= F321), E346 (= E323), F364 (vs. gap), N368 (= N337), F369 (= F338), L372 (= L341)
- binding cobalt (ii) ion: H186 (= H162), H260 (= H241), E346 (= E323)
8i15A Crystal structure of athppd-yh20335 complex
29% identity, 79% coverage: 64:346/358 of query aligns to 91:377/381 of 8i15A
- binding cobalt (ii) ion: H186 (= H162), H260 (= H241), E346 (= E323)
- binding 1,4-dimethyl-5-(2-oxidanyl-6-oxidanylidene-cyclohexen-1-yl)carbonyl-3-(phenylmethyl)benzimidazol-2-one: H186 (= H162), L217 (= L200), S219 (= S202), Q245 (= Q226), I246 (= I227), Q259 (= Q240), H260 (= H241), F333 (= F312), F344 (= F321), E346 (= E323), F364 (vs. gap), G365 (vs. gap), F369 (= F338)
Query Sequence
>AO353_26925 FitnessBrowser__pseudo3_N2E3:AO353_26925
MADLYENPMGLMGFEFIELASPTPNTLEPIFEIMGFTKVATHRSKDVHLYRQGQINLILN
NEPHSVASYFAAEHGPSVCGMAFRVKDSQKAYKRALELGAQPIHIETGPMELNLPAIKGI
GGAPLYLIDRFGEGSSIYDIDFVFIEGVDRHPVGAGLKIIDHLTHNVYRGRMAYWANFYE
KLFNFREIRYFDIKGEYTGLTSKAMTAPDGMIRIPLNEESSKGAGQIEEFLMQFNGEGIQ
HVAFLSDDLIKTWDHLKKIGMRFMTAPPDTYYEMLEGRLPNHGEPVNELQARGILLDGSS
EAGDKRLLLQIFSETLMGPVFFEFIQRKGDDGFGEGNFKALFESIERDQVRRGVLSTE
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory