SitesBLAST
Comparing AO353_27535 FitnessBrowser__pseudo3_N2E3:AO353_27535 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4pzeA Crystal structure of (s)-3-hydroxybutyryl-coa dehydrogenase paah1 in complex with acetoacetyl-coa (see paper)
62% identity, 99% coverage: 2:282/283 of query aligns to 1:281/283 of 4pzeA
4pzdA Crystal structure of (s)-3-hydroxybutyryl-coa dehydrogenase paah1 in complex with NAD+ (see paper)
62% identity, 99% coverage: 2:282/283 of query aligns to 1:281/283 of 4pzdA
- active site: S118 (= S119), H139 (= H140), E151 (= E152), N189 (= N190)
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), T12 (= T13), M13 (= M14), D32 (= D33), A88 (= A89), A89 (= A90), T90 (= T91), E91 (= E92), I99 (≠ V100), N116 (= N117), S118 (= S119), N142 (= N143)
4kugA Crystal structure of 3-hydroxybutylryl-coa dehydrogenase with NAD from clostridium butyricum
49% identity, 99% coverage: 3:282/283 of query aligns to 1:280/282 of 4kugA
- active site: S117 (= S119), H138 (= H140), E150 (= E152), N188 (= N190)
- binding nicotinamide-adenine-dinucleotide: G10 (= G12), T11 (= T13), M12 (= M14), R30 (≠ L32), D31 (= D33), A87 (= A89), A88 (= A90), E90 (= E92), N115 (= N117), S117 (= S119), H138 (= H140)
4kuhA Crystal structure of 3-hydroxybutylryl-coa dehydrogenase with acetoacetyl-coa from clostridium butyricum
49% identity, 99% coverage: 3:282/283 of query aligns to 1:280/280 of 4kuhA
6aa8E Crystal structure of (s)-3-hydroxybutyryl-coenzymea dehydrogenase from clostridium acetobutylicum complexed with NAD+ (see paper)
49% identity, 99% coverage: 4:282/283 of query aligns to 1:279/281 of 6aa8E
- active site: S116 (= S119), H137 (= H140), E149 (= E152), N187 (= N190)
- binding nicotinamide-adenine-dinucleotide: I6 (= I9), G9 (= G12), T10 (= T13), M11 (= M14), R29 (≠ L32), D30 (= D33), I31 (= I34), A86 (= A89), A87 (= A90), E89 (= E92), K94 (= K97), N114 (= N117), S116 (= S119)
P9WNP7 3-hydroxybutyryl-CoA dehydrogenase; Beta-hydroxybutyryl-CoA dehydrogenase; BHBD; EC 1.1.1.157 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
42% identity, 99% coverage: 3:282/283 of query aligns to 5:286/286 of P9WNP7
- S122 (= S119) mutation to A: Loss of fatty acyl dehydrogenase activity.
9jheA 3-hydroxybutyryl-coa dehydrogenase with NAD (see paper)
43% identity, 98% coverage: 5:282/283 of query aligns to 2:278/289 of 9jheA
- binding nicotinamide-adenine-dinucleotide: I6 (= I9), G7 (= G10), G9 (= G12), T10 (= T13), M11 (= M14), D31 (= D33), I32 (= I34), K74 (≠ D76), A87 (= A89), A88 (= A90), F89 (≠ T91), E90 (= E92), N115 (= N117), S117 (= S119), H136 (= H140)
1f0yA L-3-hydroxyacyl-coa dehydrogenase complexed with acetoacetyl-coa and NAD+ (see paper)
41% identity, 99% coverage: 3:282/283 of query aligns to 4:290/291 of 1f0yA
- active site: S126 (= S119), H147 (= H140), E159 (= E152), N197 (= N190)
- binding acetoacetyl-coenzyme a: S50 (≠ N49), K57 (≠ A57), S126 (= S119), H147 (= H140), F149 (= F142), N150 (= N143), P151 (= P144), P153 (= P146), V154 (= V147), N197 (= N190), P232 (= P225), M233 (≠ I226), L238 (= L231)
- binding nicotinamide-adenine-dinucleotide: G13 (= G12), L14 (≠ T13), M15 (= M14), D34 (= D33), Q35 (≠ I34), A96 (= A89), I97 (≠ A90), E99 (= E92), K104 (= K97), N124 (= N117), S126 (= S119), H147 (= H140), N150 (= N143), V242 (≠ I235), T246 (= T239), K282 (= K274)
9jhyB 3-hydroxybutyryl-coa dehydrogenase mutant (s117a) with acetoacetyl coa (see paper)
43% identity, 98% coverage: 5:282/283 of query aligns to 2:274/285 of 9jhyB
- binding acetoacetyl-coenzyme a: L51 (≠ Q53), K54 (= K56), K56 (≠ S58), I57 (≠ L59), H132 (= H140), F134 (= F142), N135 (= N143), R139 (≠ V147), N182 (= N190), L185 (= L193), M218 (≠ I226), I227 (= I235)
1f17A L-3-hydroxyacyl-coa dehydrogenase complexed with nadh (see paper)
41% identity, 99% coverage: 3:282/283 of query aligns to 4:290/293 of 1f17A
- active site: S126 (= S119), H147 (= H140), E159 (= E152), N197 (= N190)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G13 (= G12), L14 (≠ T13), M15 (= M14), D34 (= D33), Q35 (≠ I34), A96 (= A89), I97 (≠ A90), E99 (= E92), K104 (= K97), N124 (= N117), S126 (= S119), H147 (= H140)
1f12A L-3-hydroxyacyl-coa dehydrogenase complexed with 3-hydroxybutyryl-coa (see paper)
41% identity, 99% coverage: 3:282/283 of query aligns to 4:290/293 of 1f12A
- active site: S126 (= S119), H147 (= H140), E159 (= E152), N197 (= N190)
- binding 3-hydroxybutanoyl-coenzyme a: K57 (≠ A57), S126 (= S119), H147 (= H140), F149 (= F142), N150 (= N143), M155 (= M148), N197 (= N190), L200 (= L193), P232 (= P225), M233 (≠ I226), L238 (= L231)
P00348 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; L-3-hydroxyacyl CoA dehydrogenase; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Sus scrofa (Pig) (see paper)
40% identity, 99% coverage: 3:282/283 of query aligns to 27:313/314 of P00348
- GGGLMG 34:39 (≠ GAGTMG 10:15) binding NAD(+)
- D57 (= D33) binding NAD(+)
- E122 (= E92) binding NAD(+)
- K127 (= K97) binding NAD(+)
- S149 (= S119) binding NAD(+)
Q16836 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Homo sapiens (Human) (see 7 papers)
41% identity, 99% coverage: 3:282/283 of query aligns to 27:313/314 of Q16836
- GGGLMG 34:39 (≠ GAGTMG 10:15) binding NAD(+)
- A40 (≠ N16) to T: in HADH deficiency; dbSNP:rs137853101
- D57 (= D33) binding NAD(+); to E: in HADH deficiency; dbSNP:rs137853102; to G: found in a patient with Reye-like syndrome; does not affect 3-hydroxyacyl-CoA dehydrogenase activity; increases KM value for NADH; does not affect dimerization
- S73 (≠ N49) binding CoA
- K80 (≠ A57) binding CoA
- L86 (≠ Q63) to P: in dbSNP:rs4956145
- E122 (= E92) binding NAD(+)
- K127 (= K97) binding NAD(+); modified: N6-(2-hydroxyisobutyryl)lysine
- S149 (= S119) binding CoA; binding NAD(+)
- Q152 (≠ S122) to H: in dbSNP:rs1051519
- N173 (= N143) binding NAD(+)
- Y226 (≠ M196) to H: found in a patient with Reye-like syndrome; loss of 3-hydroxyacyl-CoA dehydrogenase activity. Does not affect dimerization; dbSNP:rs146036912
- P258 (= P228) to L: in HHF4; loss of 3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137853103
- K305 (= K274) binding NAD(+)
P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
35% identity, 99% coverage: 2:282/283 of query aligns to 360:639/763 of P40939
- E510 (= E152) active site, For hydroxyacyl-coenzyme A dehydrogenase activity; to Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769
Sites not aligning to the query:
- 282 V → D: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
- 305 I → N: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
- 342 L → P: in LCHAD deficiency; dbSNP:rs137852772
6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
38% identity, 92% coverage: 25:283/283 of query aligns to 328:584/707 of 6yswA
Sites not aligning to the query:
- active site: 66, 71, 84, 88, 112, 115, 136, 137, 145, 264
- binding coenzyme a: 23, 25, 66, 67, 68, 136, 137, 140, 290, 293
7o4uA Structure of the alpha subunit of mycobacterium tuberculosis beta- oxidation trifunctional enzyme in complex with oxidized nicotinamide adenine dinucleotide (see paper)
36% identity, 99% coverage: 3:282/283 of query aligns to 316:598/711 of 7o4uA