SitesBLAST
Comparing AO356_01235 FitnessBrowser__pseudo5_N2C3_1:AO356_01235 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 15 hits to proteins with known functional sites (download)
P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
24% identity, 91% coverage: 14:423/449 of query aligns to 4:417/430 of P0AA76
- Y29 (= Y39) binding
- D31 (= D41) mutation to N: Loss of galactonate transport activity.
- R32 (= R42) binding
- Y64 (= Y74) binding
- E118 (= E128) mutation to Q: Loss of galactonate transport activity.
- W358 (= W363) binding
6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
24% identity, 89% coverage: 24:423/449 of query aligns to 1:398/409 of 6e9nA
P53322 High-affinity nicotinic acid transporter; Nicotinic acid permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
22% identity, 72% coverage: 12:333/449 of query aligns to 77:397/534 of P53322
- K283 (≠ R218) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
23% identity, 89% coverage: 24:423/449 of query aligns to 5:382/393 of 6e9oA
Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
23% identity, 81% coverage: 60:423/449 of query aligns to 105:472/495 of Q9NRA2
- K136 (≠ R91) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
- H183 (≠ I136) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
- LL 198:199 (≠ AI 151:152) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- IL 266:267 (≠ LV 224:225) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- SSLRN 268:272 (≠ KAIDD 226:230) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
- G328 (= G291) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
- P334 (= P297) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
- G371 (≠ A320) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172
Sites not aligning to the query:
- 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
- 39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794
Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
23% identity, 77% coverage: 48:394/449 of query aligns to 90:441/495 of Q5Q0U0
- K136 (≠ R91) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
- R168 (= R121) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
- E171 (≠ L124) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
- G172 (= G125) mutation to C: Decreases protein levels and alters subcellular localization.
- E175 (= E128) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
- G176 (≠ A129) mutation to C: Decreases protein levels and alters subcellular localization.
- F179 (= F132) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
- P180 (= P133) mutation to C: Decreases protein levels and alters subcellular localization.
- H183 (≠ I136) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
- W186 (≠ F139) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
- SSLKN 268:272 (≠ KAIDD 226:230) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
- P334 (= P297) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
- G371 (≠ A320) mutation to V: Remains in the endoplasmic reticulum.
Sites not aligning to the query:
- 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
- 39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
22% identity, 77% coverage: 48:394/449 of query aligns to 90:441/495 of Q8BN82
- H183 (≠ I136) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.
Sites not aligning to the query:
- 39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.
Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
21% identity, 75% coverage: 68:403/449 of query aligns to 129:470/582 of Q9JI12
- R184 (= R121) mutation R->A,E,K: Greatly lowers L-glutamate transport.
- E191 (= E128) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
- R322 (≠ I264) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.
Sites not aligning to the query:
- 88 R→A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
- 128 H→A: Greatly lowers L-glutamate transport.
7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
21% identity, 75% coverage: 68:403/449 of query aligns to 71:412/452 of 7t3nA
Sites not aligning to the query:
Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
23% identity, 45% coverage: 84:283/449 of query aligns to 80:285/452 of Q5EXK5
- D82 (≠ Q86) mutation to A: Loss of activity.
Sites not aligning to the query:
- 311 V→W: Loss of activity.
- 314 D→A: Loss of activity.
Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
24% identity, 51% coverage: 70:299/449 of query aligns to 90:318/493 of Q03567
Sites not aligning to the query:
- 69 modified: carbohydrate, N-linked (GlcNAc...) asparagine
Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
25% identity, 41% coverage: 119:303/449 of query aligns to 168:343/497 of Q9Y2C5
Sites not aligning to the query:
- 66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
- 75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
- 90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
- 372 A → T: in dbSNP:rs11754288
Q9C0U9 Uncharacterized transporter PB1C11.03 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
21% identity, 58% coverage: 54:313/449 of query aligns to 130:394/570 of Q9C0U9
Sites not aligning to the query:
- 14 modified: Phosphoserine
Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
23% identity, 58% coverage: 25:283/449 of query aligns to 29:294/448 of Q51955
- D41 (≠ N38) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
- D44 (= D41) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
- G85 (≠ N82) mutation to V: Abolishes 4-HBA transport and chemotaxis.
- D89 (≠ Q86) mutation to N: Abolishes 4-HBA transport and chemotaxis.
- G92 (= G89) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
- R124 (= R121) mutation to A: Abolishes 4-HBA transport.
- E144 (≠ R141) mutation to A: Strong decrease in 4-HBA transport.
- H183 (≠ Q185) mutation to A: Decrease in 4-HBA transport and chemotaxis.
Sites not aligning to the query:
- 323 D→N: Abolishes 4-HBA transport and chemotaxis.
- 328 H→A: Decrease in 4-HBA transport and chemotaxis.; H→R: Decrease in 4-HBA transport and loss of chemotaxis.
- 386 R→A: Strong decrease in 4-HBA transport.
- 398 R→A: Abolishes 4-HBA transport.
- 444 H→A: No change in 4-HBA transport and chemotaxis.
A0A0H2VG78 Glucose transporter GlcP; Glucose/H(+) symporter from Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) (see paper)
24% identity, 59% coverage: 52:314/449 of query aligns to 33:304/446 of A0A0H2VG78
- R102 (= R121) mutation to A: Loss of transport activity.
- I105 (≠ L124) mutation to S: Affects symport activity. May function as an uniporter.
- E122 (≠ R141) mutation to A: Loss of transport activity.
- Q137 (≠ A162) mutation to A: Loss of transport activity.
- Q250 (≠ I264) mutation to A: Loss of transport activity.
- Q251 (= Q265) mutation to A: Loss of transport activity.
- N256 (≠ Y269) mutation to A: Loss of transport activity.
Sites not aligning to the query:
- 22 D→N: Affects symport activity. May function as an uniporter.
- 357 W→A: Loss of transport activity.
Query Sequence
>AO356_01235 FitnessBrowser__pseudo5_N2C3_1:AO356_01235
VNTSSLKANAGADPVLLSAIAKVKRHILPLFVIMFIVNYIDRVNIGFVRAHMEHDLGIGA
AAYGFGAGLFFIGYALFEVPSNILLQRIGARIWLTRIMLTWGLVAAAMAFIQNETHFYIL
RFLLGVAEAGFFPGVIYYFTRWLPGVERGKAIAIFLSGSAIASLISGPLSGLLLQINGLG
MHGWQWMYFIEGMFSVCLCVFVWFWLDSKPHDAKWLSREEQDVLVKAIDDEQRAREAASP
IKLSIGQLLKDRQIILFCLIYFFIQLTIYAATFWLPSIIKKMGELSDIQVGLFNSIPWLL
SIVGMYAFASLSAKWKFQQAWVATALLIAAAGMFMSTTGGPIFAFVAICFAALGFKSASS
LFWPIPQAYLDARIAAGVIALINSVGNLGGFVAPTTFGLLEEHTGSIQGGLYGLAATSII
AAIIVFAARNTPKPKAVTTTPVDPAASHV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory