SitesBLAST

P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
25% identity, 78% coverage: 3:351/449 of query aligns to 2:353/457 of P15993

query
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P15993

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Y103 (= Y104) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.

Q9FFL1 Polyamine transporter RMV1; Protein RESISTANT TO METHYL VIOLOGEN 1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
24% identity, 71% coverage: 34:351/449 of query aligns to 70:381/490 of Q9FFL1

query
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Q9FFL1

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I377 (≠ L347) mutation to F: Loss of sensitivity to paraquat.

P24207 Phenylalanine-specific permease; Phenylalanine:H(+) symporter PheP from Escherichia coli (strain K12) (see 3 papers)
26% identity, 63% coverage: 68:351/449 of query aligns to 75:361/458 of P24207

query
sites
P24207

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F87 (≠ V80) mutation to L: No effect on phenylalanine transport activity.
F90 (≠ Y83) mutation to L: 65% of wild-type phenylalanine transport activity.
Y92 (≠ Q85) mutation to L: 41% of wild-type phenylalanine transport activity.
Y94 (≠ G87) mutation to L: 69% of wild-type phenylalanine transport activity.
W95 (≠ L88) mutation to L: 10% of wild-type phenylalanine transport activity.
F98 (≠ H91) mutation to L: No effect on phenylalanine transport activity.
F101 (= F94) mutation to L: 38% of wild-type phenylalanine transport activity.
W105 (= W98) mutation to L: 39% of wild-type phenylalanine transport activity.
Y107 (≠ M100) mutation to L: No effect on phenylalanine transport activity.
W108 (≠ L101) mutation to L: 71% of wild-type phenylalanine transport activity.
F111 (≠ Y104) mutation to L: 60% of wild-type phenylalanine transport activity.; mutation to Y: Enables the transport of tryptophan to almost the same steady-state level as that of phenylalanine.
E118 (≠ L111) mutation E->G,L,V,N: Loss of activity.
K168 (≠ A160) mutation K->L,R: Strong decrease in phenylalanine transport activity.; mutation to N: Loss of activity.
E226 (≠ D216) mutation E->A,Q,K,R,W: Loss of activity.
R252 (≠ L238) mutation R->D,E,F,W,P: Loss of activity.
P341 (= P334) mutation to A: 5% of wild-type phenylalanine transport activity.; mutation P->G,Q,K,R: Loss of activity.; mutation to S: 3% of wild-type phenylalanine transport activity.; mutation to T: 17% of wild-type phenylalanine transport activity.

Sites not aligning to the query:

26 mutation R->G,S,Q: Strong decrease in phenylalanine transport activity.
54 P→A: 50% of wild-type phenylalanine transport activity.; P→G: No change in phenylalanine transport activity.; P→L: 26% of wild-type phenylalanine transport activity.
442 P→A: 46% of wild-type phenylalanine transport activity.; P→G: 52% of wild-type phenylalanine transport activity.; P→L: 43% of wild-type phenylalanine transport activity.

P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
22% identity, 84% coverage: 3:379/449 of query aligns to 19:428/629 of P30825

query
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P30825

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G

-

F

-

V

-

K

-

G

-

S

-

V

-

K

-

N

W

W

N

Q

A

L

L

T

H

E

G

E

G

D

A

F

G

G

H
x
N

-

T

-

S

G

G

Q

R

L

L

T

C

L

L

A

N

P

N

L

D

Y

T

S

K

P

E

E

G

H

K

-

P

-

G

-

V

-

G

-

F

-

M

-

P

F

F

N

G

F

F

A

S

L

G

L

V

M

L

Q

S

A

G

V

A

S

A

I

T

A

C

V

F

L

Y

S

A

F

F

L

V

G

G

F

F

D

D

A

C

I

I

S

A

T

T

L

T

A

G

E

E

I

V

K

K

G

-

D

N

P

P

G

Q

R

K

S

A

V

I

G

P

K

V

A

G

A

I

L

V

V

A

T

S

L

L

V

I

M

-

G

-

A

-

I

C

F

F

V

I

V

A

Q

Y

T

F

W

G

I

V

A

S

T

A

D

A

L

L

A

T

A

L

G

M

M

M

G

P

F

Y

K

F

S

C

A

L

D

D

T

N

A

N

F

S

Y

P

E

L

I

P

A

D

E

A

L

F

A

K

A

H

G

V

S

G

W

W

L

E

A

G

T

A

L

K

T

Y

A

A

V

V

A

A

T

V

-

G

A

S

L

L

A

C

W

A

G

L

V

S

A

A

V

S

A

L

I

L

T

G

S

S

Q

M

A

F

A

P

V

M

S

P

R

R

L

V

L

I

F

Y

G

A

M

M

A

A

R

E

D

D

G

G

Q

L

L

L

P

F

K

K

V

F

L

L

A

A

K

N

V

V

H

N

P

D

K

R

H

T

N

K

T

T

P

P

Y

I

M

I

S

A

I

T

Y

L

L

A

V

S

A

G

V

A

L

V

S

A

L

A

L

V

I

M

C

A

Y

F

L

L

F

F

I

-

N

-

A

D

V

L

D

K

T

D

L

L

T

V

S

D

L

L

V

M

N

S

F

I

G

G

A

T

L

L

S

L

G

A

F

Y

M

S

L

L

L

V

H

A

I

A

T

C

V

V

I

L

N226 (≠ H182) modified: carbohydrate, N-linked (GlcNAc...) asparagine

Q9URZ4 Cationic amino acid transporter 1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
24% identity, 74% coverage: 45:376/449 of query aligns to 114:463/587 of Q9URZ4

query
sites
Q9URZ4

A

G

P

P

G

A

M

S

V

V

P

I

L

I

A

N

Y

Y

I

S

I

L

G

I

M

G

V

I

A

M

M

M

L

F

F

F

T

I

A

V

L

Y

S

A

Y

L

G

G

S

E

M

M

A

A

R

V

A

A

F

Y

P

P

V

V

A

A

G

G

S

G

V

F

Y

N

S

T

Y

Y

A

A

Q

T

R

R

G

F

L

I

N

D

P

P

H

A

V

W

G

G

F

F

I

A

A

V

G

S

W

W

L

N

M

Y

L

F

L

I

D

N

Y

Y

L

F

L

V

I

T

P

F

P

P

L

L

L

E

Y

L

V

T

Y

T

A

C

A

A

M

I

A

T

L

F

N

R

H

Y

L

-

Y

W

P

T

D

D

I

I

P

N

K

S

V

A

G

A

F

W

I

I

L

S

A

I

F

F

L

L

V

V

S

V

A

I

T

I

F

I

V

V

N

N

L

L

R

F

G

G

I

V

T

-

F

-

T

R

A

A

R

Y

M

G

N

E

I

V

I

E

F

F

L

I

L

L

A

S

Q

T

L

V

V

K

V

V

L

V

G

A

I

T

F

F

L

G

F

F

Y

I

A

I

W

L

N

A

A

I

L

I

-

I

-

N

-

C

-

G

-

V

-

P

-

T

-

D

H

H

G

R

G

G

A

Y

G

I

H

G

G

G

Q

S

L

I

T

I

L

-

A

-

P

-

L

K

Y

H

S

K

P

P

E

F

H

R

F

H

N

G

F

F

A

K

L

G

L

F

M

C

Q

S

A

V

V

F

S

T

I

T

A

A

V

A

L

F

S

S

F

F

L

S

G

G

F

T

D

E

A

V

I

I

S

G

T

L

L

A

A

A

E

A

E

E

I

V

K

D

G

-

D

N

P

P

G

Q

R

K

S

A

V

L

G

P

K

H

A

A

A

V

L

K

V

Q

T

V

L

F

L

W

V

R

M

I

G

A

A

I

I

F

F

Y

V

V

Q

S

-

L

-

I

-

L

-

I

-

G

T

L

W

L

I

I

A

S

T

P

D

D

L

D

A

P

A

N

G

L

M

M

G

G

F

N

K

G

S

S

A

T

D

S

T

V

A

S

F

P

Y

F

E

V

I

L

A

A

E

I

L

K

A

E

A

A

G

N

S

-

W

-

L

I

A

K

T

G

L

L

T

P

A

S

V

V

A

F

T

N

A

A

L

V

A

I

W

I

G

I

V

S

A

V

V

V

A

S

I

V

T

T

S

N

Q

S

A

S

A

T

V

Y

S

T

-

A

-

G

R

R

L

T

L

L

F

H

G

G

M

M

A

A

R

N

D

L

G

K

Q

Q

L

A

P

P

K

S

V

F

L

F

A

-

K

K

V

Y

H

T

P

D

K

R

H

L

N

G

T

R

P

P

Y

L

M

L

S

A

I

M

Y

I

L

V

V

V

A

L

V

L

L

F

S

G

L

F

L

-

I

-

C

-

Y

F

L

A

F

Y

I

I

N

N

A

E

V

A

D

D

T

K

-

N

-

G

-

N

-

D

-

V

-

S

-

D

-

T

-

V

-

F

-

N

-

W

-

L

-

A

L

L

T

S

S

G

L

L

V

S

N

N

F

F

G

F

A

T

L

W

S

G

G

S

F

I

M

C

L

L

L

C

H

H

I

I

Sites not aligning to the query:

29 modified: Phosphoserine
30 modified: Phosphoserine
37 modified: Phosphoserine

7cmiB The lat2-4f2hc complex in complex with leucine (see paper)
22% identity, 78% coverage: 44:395/449 of query aligns to 27:380/458 of 7cmiB

query
sites
7cmiB

E

E

A

N

P

A

G

G

M

S

V

V

P

G

L

L

A

A

Y

L

I

I

I

V

G

W

M

I

V

V

A

T

M

G

L

F

F

I

T

T

-

V

-

G

A

A

L

L

S

C

Y

Y

G

A

S

E

M

L

A

G

R

V

A

T

F

I

P

P

V

K

A

S

G

G

S

G

V

D

Y

Y

S

S

Y

Y

A

V

Q

K

R

D

G

I

L

F

N

G

P

G

H

L

V

A

G

G

F

F

I

L

A

R

G

L

W

W

L

I

M

A

L

V

L

L

D

-

Y

-

L

-

L

V

I

I

P

Y

P

P

-

T

-
x
N

-

Q

-

A

-

V

L

I

L

A

Y

L

V

T

Y

F

A

S

A

N

M

Y

A

V

L

L

N

Q

H

P

L

L

Y

F

P

P

D

T

I

C

-

F

-

P

P

P

K

E

V

S

G

G

F

L

I

R

L

L

A

L

F

A

L

A

V

I

S

C

A

L

-

L

T

T

F

W

V

V

N

N

L

C

R

S

G

S

I

V

T

R

F

W

T

A

A

T

R

R

M

V

N

Q

I

D

I

I

F

F

L

T

L

A

A

G

Q

K

L

L

V

L

V

A

L

L

G

A

I

L

F

I

L

I

F

I

Y

M

A

G

W

I

N

V

A

Q

L

I

H

C

G

K

G

G

A

E

G

-

H

-

G

-

Q

Y

L

F

T

W

L

L

A

E

P

P

L

K

Y

N

S

A

P

F

E

E

H

N

F

F

-

Q

-

E

-

P

N

D

F

I

A

G

L

L

L

V

M

A

Q

L

A

A

V

F

S

L

I

Q

A

G

V

S

L
x
F

S

A

F

Y

L
x
G

G

G

F

W

D

N

A

F

I

L

S

N

T

Y

L

V

A

T

E

E

I

L

K

V

G

-

D

D

P

P

G

Y

R

K

S

N

V

L

G

P

K

R

A

A

A

I

L

F

V

I

T

S

L

I

L

P

V

L

M

V

G

T

A

F

I

V

F

Y

V

V

V

F

Q

A

T

N

W

V

I

A

A

Y

T

V

D

T

L

A

A

M

A

S

G

P

M

Q

G

E

F

L

K

L

S

A

A

S

D

N

T

A

A

V

F

A

Y

V

E

T

I

F

A

G

E

E

-

K

-

L

L

L

A

G

A

V

G

M

S

A

W

W

L

I

A

M

T

P

L

I

T

S

A

-

V

V

A

A

T

L

A

S

L

T

A

F

W

G

G

G

V

V

A

N

V

G

A

S

I

L

T

F

S

T

Q

S

A

-

V

-

S

S

R

R

L

L

L

F

F

F

G

A

M

G

A

A

R

R

D

E

G

G

Q

H

L

L

P

P

K

S

V

V

L

L

A

A

K

M

V

I

H

H

P

V

K

K

H

R

N

C

T

T

P

P

Y

I

M

P

S

A

I

L

Y

L

L

F

V

T

A

C

V

I

L

S

S

T

L

L

I

M

C

L

Y

-

L

-

F

-

I

-

N

-

A

V

V

T

D

S

T

D

L

M

T

Y

S

T

L

L

V

I

N

N

F

Y

G

V

A

G

L

F

S

I

G

N

F

Y

M

L

L

F

L

Y

H

G

I

V

T

T

V

V

I

A

N

G

H

Q

Y

I

W

V

R

L

R

R

Q

W

R

K

S

K

G

P

Q

D

L

I

I

P

R

R

H

P

V

I

binding leucine: N94 (vs. gap), F203 (≠ L210), G206 (≠ L213)

Sites not aligning to the query:

binding leucine: 13, 15, 17

7cmhB The lat2-4f2hc complex in complex with tryptophan (see paper)
22% identity, 78% coverage: 44:395/449 of query aligns to 27:380/458 of 7cmhB

query
sites
7cmhB

E

E

A

N

P

A

G

G

M

S

V

V

P

G

L

L

A

A

Y

L

I

I

I

V

G

W

M

I

V

V

A

T

M

G

L

F

F

I

T

T

-

V

-

G

A

A

L

L

S

C

Y

Y

G

A

S

E

M

L

A

G

R

V

A

T

F

I

P

P

V

K

A

S

G

G

S

G

V

D

Y

Y

S

S

Y

Y

A

V

Q

K

R

D

G

I

L

F

N

G

P

G

H

L

V

A

G

G

F

F

I

L

A

R

G

L

W

W

L

I

M

A

L

V

L

L

D

-

Y

-

L

-

L

V

I

I

P

Y

P

P

-

T

-
x
N

-

Q

-

A

-

V

L

I

L

A

Y

L

V

T

Y

F

A

S

A

N

M

Y

A

V

L

L

N

Q

H

P

L

L

Y

F

P

P

D

T

I

C

-

F

-

P

P

P

K

E

V

S

G

G

F

L

I

R

L

L

A

L

F

A

L

A

V

I

S

C

A

L

-

L

T

T

F

W

V

V

N

N

L

C

R

S

G

S

I

V

T

R

F

W

T

A

A

T

R

R

M

V

N

Q

I

D

I

I

F

F

L

T

L

A

A

G

Q

K

L

L

V

L

V

A

L

L

G

A

I

L

F

I

L

I

F

I

Y

M

A

G

W

I

N

V

A

Q

L

I

H

C

G

K

G

G

A

E

G

-

H

-

G

-

Q

Y

L

F

T

W

L

L

A

E

P

P

L

K

Y

N

S

A

P

F

E

E

H

N

F

F

-

Q

-

E

-

P

N

D

F

I

A

G

L

L

L

V

M

A

Q

L

A

A

V

F

S

L

I

Q

A

G

V

S

L
x
F

S
x
A

F

Y

L
x
G

G

G

F

W

D

N

A

F

I

L

S

N

T

Y

L

V

A

T

E

E

I

L

K

V

G

-

D

D

P

P

G

Y

R

K

S

N

V

L

G

P

K

R

A

A

A

I

L

F

V

I

T

S

L

I

L

P

V

L

M

V

G

T

A

F

I

V

F

Y

V

V

V

F

Q

A

T

N

W

V

I

A

A

Y

T

V

D

T

L

A

A

M

A

S

G

P

M

Q

G

E

F

L

K

L

S

A

A

S

D

N

T

A

A

V

F

A

Y

V

E

T

I

F

A

G

E

E

-

K

-

L

L

L

A

G

A

V

G

M

S

A

W

W

L

I

A

M

T

P

L

I

T

S

A

-

V

V

A

A

T

L

A

S

L

T

A

F

W

G

G

G

V

V

A

N

V

G

A

S

I

L

T

F

S

T

Q

S

A

-

V

-

S

S

R

R

L

L

L

F

F

F

G

A

M

G

A

A

R

R

D

E

G

G

Q

H

L

L

P

P

K

S

V

V

L

L

A

A

K

M

V

I

H

H

P

V

K

K

H

R

N

C

T

T

P

P

Y

I

M

P

S

A

I

L

Y

L

L

F

V

T

A

C

V

I

L

S

S

T

L

L

I

M

C

L

Y

-

L

-

F

-

I

-

N

-

A

V

V

T

D

S

T

D

L

M

T

Y

S

T

L

L

V

I

N

N

F

Y

G

V

A

G

L

F

S

I

G
x
N

F

Y

M

L

L

F

L

Y

H

G

I

V

T

T

V

V

I

A

N

G

H

Q

Y

I

W

V

R

L

R

R

Q

W

R

K

S

K

G

P

Q

D

L

I

I

P

R

R

H

P

V

I

binding tryptophan: N94 (vs. gap), F203 (≠ L210), A204 (≠ S211), G206 (≠ L213), N355 (≠ G370)

Sites not aligning to the query:

binding tryptophan: 15, 17

7b00A Human lat2-4f2hc complex in the apo-state (see paper)
22% identity, 78% coverage: 44:395/449 of query aligns to 27:380/457 of 7b00A

query
sites
7b00A

E

E

A

N

P

A

G

G

M

S

V

V

P

G

L

L

A

A

Y

L

I

I

I

V

G

W

M

I

V

V

A

T

M

G

L

F

F

I

T

T

-

V

-

G

A

A

L

L

S

C

Y

Y

G

A

S

E

M

L

A

G

R

V

A

T

F

I

P

P

V

K

A

S

G

G

S

G

V

D

Y

Y

S

S

Y

Y

A

V

Q

K

R

D

G

I

L

F

N

G

P

G

H

L

V

A

G

G

F

F

I

L

A

R

G

L

W

W

L

I

M

A

L

V

L

L

D

-

Y

-

L

-

L

V

I

I

P

Y

P

P

-

T

-

N

-

Q

-

A

-

V

L

I

L

A

Y

L

V

T

Y

F

A

S

A

N

M

Y

A

V

L

L

N

Q

H

P

L

L

Y

F

P

P

D

T

I

C

-

F

-

P

P

P

K

E

V

S

G

G

F

L

I

R

L

L

A

L

F

A

L

A

V

I

S

C

A

L

-

L

T

T

F

W

V

V

N

N

L

C

R

S

G

S

I

V

T

R

F

W

T

A

A

T

R

R

M

V

N

Q

I

D

I

I

F

F

L

T

L

A

A

G

Q

K

L

L

V

L

V

A

L

L

G

A

I

L

F

I

L

I

F

I

Y

M

A

G

W

I

N

V

A

Q

L

I

H

C

G

K

G

G

A

E

G

-

H

-

G

-

Q

Y

L

F

T

W

L

L

A

E

P

P

L

K

Y

N

S

A

P

F

E

E

H

N

F

F

-

Q

-

E

-

P

N

D

F

I

A

G

L

L

L

V

M

A

Q

L

A

A

V

F

S

L

I

Q

A

G

V

S

L

F

S

A

F

Y

L

G

G

G

F

W

D

N

A

F

I

L

S

N

T

Y

L

V

A

T

E

E

I

L

K

V

G

-

D

D

P

P

G

Y

R

K

S

N

V

L

G

P

K

R

A

A

A

I

L

F

V

I

T

S

L

I

L

P

V

L

M

V

G

T

A

F

I

V

F

Y

V

V

V

F

Q

A

T

N

W

V

I

A

A

Y

T

V

D

T

L

A

A

M

A

S

G

P

M

Q

G

E

F

L

K

L

S

A

A

S

D

N

T

A

A

V

F

A

Y

V

E

T

I

F

A

G

E

E

-

K

-

L

L

L

A

G

A

V

G

M

S

A

W

W

L

I

A

M

T

P

L

I

T

S

A

-

V

V

A

A

T

L

A

S

L

T

A

F

W

G

G

G

V

V

A

N

V

G

A

S

I

L

T

F

S

T

Q

S

A

-

V

-

S

S

R

R

L

L

L

F

F

F

G

A

M

G

A

A

R

R

D

E

G

G

Q

H

L

L

P

P

K

S

V

V

L

L

A

A

K

M

V

I

H

H

P

V

K

K

H

R

N

C

T

T

P

P

Y

I

M

P

S

A

I

L

Y

L

L

F

V

T

A

C

V

I

L

S

S

T

L

L

I
x
M

C

L

Y

-

L

-

F

-

I

-

N

-

A

V

V

T

D

S

T

D

L

M

T

Y

S

T

L

L

V

I

N

N

F
x
Y

G

V

A

G

L

F

S

I

G

N

F

Y

M

L

L

F

L

Y

H

G

I

V

T

T

V

V

I

A

N

G

H

Q

Y

I

W

V

R

L

R

R

Q

W

R

K

S

K

G

P

Q

D

L

I

I

P

R

R

H

P

V

I

binding Digitonin: M338 (≠ I348), Y350 (≠ F365)

Sites not aligning to the query:

binding Digitonin: 408, 411

Q9UHI5 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; hLAT2; Solute carrier family 7 member 8 from Homo sapiens (Human) (see 3 papers)
22% identity, 78% coverage: 44:395/449 of query aligns to 67:420/535 of Q9UHI5

query
sites
Q9UHI5

E

E

A

N

P

A

G

G

M

S

V

V

P

G

L

L

A

A

Y

L

I

I

I

V

G

W

M

I

V

V

A

T

M

G

L

F

F

I

T

T

-

V

-

G

A

A

L

L

S

C

Y
|
Y

G

A

S

E

M

L

A

G

R

V

A

T

F

I

P

P

V

K

A

S

G

G

S

G

V

D

Y

Y

S

S

Y

Y

A

V

Q

K

R

D

G

I

L

F

N

G

P

G

H

L

V

A

G

G

F

F

I

L

A

R

G

L

W

W

L

I

M

A

L

V

L

L

D

-

Y

-

L

V

L

I

I

Y

P

P

P

T

-
x
N

-

Q

-

A

-

V

L

I

L

A

Y

L

V

T

Y

F

A

S

A

N

M

Y

A

V

L

L

N

Q

H

P

L

L

Y

F

P

P

D

T

I
x
C

-

F

-

P

P

P

K

E

V

S

G

G

F

L

I

R

L

L

A

L

F

A

L

A

V

I

S

C

A

L

-

L

T

T

F
x
W

V

V

N

N

L

C

R

S

G

S

I

V

T

R

F

W

T

A

A

T

R

R

M

V

N

Q

I

D

I

I

F

F

L

T

L

A

A

G

Q

K

L

L

V

L

V

A

L

L

G

A

I

L

F

I

L

I

F

I

Y

M

A

G

W

I

N

V

A

Q

L

I

H

C

G

K

G

G

A

E

G

-

H

-

G

-

Q

Y

L

F

T

W

L

L

A

E

P

P

L

K

Y

N

S

A

P

F

E

E

H

N

F

F

-

Q

-

E

-

P

N

D

F

I

A

G

L

L

L

V

M

A

Q

L

A

A

V

F

S

L

I

Q

A

G

V

S

L
x
F

S

A

F

Y

L
x
G

G

G

F

W

D

N

A

F

I

L

S

N

T

Y

L

V

A

T

E

E

I

L

K

V

G

-

D

D

P

P

G

Y

R

K

S

N

V

L

G

P

K

R

A

A

A

I

L

F

V

I

T

S

L

I

L

P

V

L

M

V

G

T

A

F

I

V

F

Y

V

V

V

F

Q

A

T

N

W

V

I

A

A

Y

T

V

D

T

L

A

A

M

A

S

G

P

M

Q

G

E

F

L

K

L

S

A

A

S

D

N

T

A

A
x
V

F

A

Y

V

E

T

I

F

A

G

E

E

-

K

-

L

L

L

A

G

A

V

G

M

S

A

W

W

L

I

A

M

T

P

L

I

T

S

A

-

V

V

A

A

T

L

A

S

L

T

A

F

W

G

G

G

V

V

A

N

V

G

A

S

I

L

T

F

S

T

Q

S

A

-

V

-

S

S

R

R

L

L

L

F

F

F

G

A

M

G

A

A

R

R

D

E

G

G

Q

H

L

L

P

P

K

S

V

V

L

L

A

A

K

M

V

I

H

H

P

V

K

K

H

R

N

C

T

T

P

P

Y

I

M

P

S

A

I

L

Y

L

L

F

V

T

A

C

V

I

L

S

S

T

L

L

I

M

C

L

Y

-

L

-

F

-

I

-

N

-

A

V

V

T

D

S

T

D

L

M

T

Y

S

T

L

L

V

I

N

N

F

Y

G

V

A

G

L

F

S

I

G
x
N

F
x
Y

M

L

L

F

L

Y

H

G

I

V

T
|
T

V

V

I

A

N

G

H

Q

Y

I

W

V

R

L

R

R

Q

W

R

K

S

K

G

P

Q

D

L

I

I

P

R
|
R

H

P

V

I

Y93 (= Y67) mutation to A: Nearly complete reduction of glycine, L-alanine, and L-glutamine uptake. Minimal effect on the transport of L-isoleucine, L-histidine and L-tryptophan.
N134 (vs. gap) Important for substrate specificity; binding ; mutation to Q: Reduces L-leucine uptake activity. Abolishes L-tryptophan uptake.; mutation to S: The substrate specificity changed dramatically reducing L-glutamine, glycine and L-alanine uptake activity thus mimicking the selectivity of SLC7A5.
C154 (≠ I126) modified: Interchain (with C-210 in SLC3A2)
W174 (≠ F141) mutation to A: Does not affect protein expression, plasma membrane localization, or L-alanine uptake.
F243 (≠ L210) mutation to A: Abolishes leucine and tryptophan transport activities.
G246 (≠ L213) Important for substrate specificity; binding ; mutation to S: Strong decrease in the uptake of large substrates L-tryptophan, L-glutamine, and L-histidine but increases the uptake of small neutral amino acids glycine and L-alanine.
V302 (≠ A270) to I: found in a patient with age-related hearing loss; does not affect L-alanine transport activity. Decreases L-tyrosine transport activity
N395 (≠ G370) binding ; mutation to Q: Strongly reduces L-leucine uptake activity. Strongly reduces L-tryptophan uptake activity.
Y396 (≠ F371) mutation to A: Strongly reduces L-leucine uptake activity.
T402 (= T377) to M: found in a patient with age-related hearing loss; strongly decreased L-alanine transport activity. Decreases L-tyrosine transport activity
R418 (= R393) to C: found in a patient with age-related hearing loss; decreases L-alanine transport activity. Decreases L-tyrosine transport activity

Sites not aligning to the query:

53 binding
460 V → E: found in a patient with age-related hearing loss; strongly decreases L-alanine transport activity. Decreases L-tyrosine transport activity. Decreases cell membrane localization

P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
24% identity, 89% coverage: 2:399/449 of query aligns to 7:410/489 of P25737

query
sites
P25737

S

T

I

T

E

E

A

A

F

P

G

G

Y

L

K

R

Q

R

E

E

L

L

K

K

R

-

G

A

L

R

S

H

L

L

T

T

D

M

L

I

V

A

V

I

Y

G

G

G

M

S

I

I

F

G

M

T

I

G

P

L

I

F

A

V

P

A

F

S

G

G

V

A

Y

T

G

-

Y

-

V

I

N

S

A

Q

E

A

A

G

P

P

G

G

M

G

V

A

P

L

L

L

A

S

Y

Y

I

M

I

L

G

I

M

G

V

L

A

M

M

V

L

Y

F

F

T

L

A

M

L

T

S

S

Y

L

G

G

S

E

M

L

A

A

R

A

A

Y

F

M

P

P

V

V

A

S

G

G

S

S

V

F

Y

A

S

T

Y

Y

A

G

Q

Q

R

N

G

Y

L

V

N

E

P

E

H

G

V

F

G

G

F

F

I

A

A

L

G

G

W

W

L

N

M
x
Y

L

W

L

Y

D

N

Y
x
W

L

A

L

V

I

T

P

I

P

A

L

V

L

D

Y

L

V

V

Y

A

A

A

A

Q

M

L

A

V

L

M

N

S

H

W

L

W

Y

F

P

P

D

D

I

T

P

P

K

G

-

W

-

I

-

W

V

S

G

A

F

L

I

F

L

L

A

G

F

V

L

I

V

F

S

L

A

L

T

N

F

Y

V

I

N

S

L

V

R

R

G

G

I

F

T

G

-

E

-

A

-

E

-

Y

-

W

-

F

-

S

-

L

F

I

T
x
K

A

V

R

T

M

T

N

V

I

I

I

V

F

F

L

I

A

V

Q

G

-

V

L

L

V

M

V

I

L

I

G

G

I

I

F

F

L

K

F

-

Y

-

A

-

W

-

N

G

A

A

L

Q

H

P

G

A

G

G

A

W

G

S

H

N

G

W

Q

T

L

I

T

G

L

E

A

A

P

P

L

F

Y

A

S

G

P

-

E

-

H

-

F

-

N

G

F

F

A

A

L

A

L

M

M

I

Q

G

A

V

V

A

S

M

I

I

A

V

V

G

L
x
F

S

S

F

F

L

Q

G

G

F

T

D
x
E

A

L

I

I

S

G

T

I

L

A

A

A

E

G

E
|
E

I

S

K

E

G

-

D

D

P

P

G

A

R

K

S

N

V

I

G

P

K

R

A

A

A

V

-

R

-

Q

-

V

-

F

-

W

L

R

V

I

T

L

L

L

L

F

V

Y

M

V

G

F

A

A

I

I

F

L

V

I

Q

S

T

L

W

I

I

I

A

P

T

Y

D

T

L

D

A

P

A

S

G

L

M

L

G

R

F

N

K
x
D

S

V

A

K

D
|
D

T

I

A

S

F

V

Y

S

E

P

I

F

A

T

E

L

L

V

A

F

A

Q

G

H

S

A

W

G

L

L

A

L

T

S

L

A

T

A

A

A

V

V

A

M

T

N

A

A

L

V

A

-

W

-

G

-

V

I

A

L

V

T

A

A

I

V

T

L

S

S

Q

A

A

G

-

N

-

S

-

G

-

M

-

Y

A

A

V

S

S

T

R

R

L

M

L

L

F

Y

G

T

M

L

A

A

R

C

D

D

G

G

Q

K

L

A

P

P

K

R

V

I

L

F

A

A

K

K

V

L

H

S

P

-

K

R

H

G

N

G

T

V

P

P

Y

R

M

N

S

A

I

L

Y

Y

L

A

V

T

A

T

V

V

L

I

S

A

L

G

L

L

I

-

C

C

Y

F

L

L

-

T

-

S

-

M

F

F

I

G

N

N

A

Q

V

T

D

V

T

Y

L

L

T

W

S

-

L

L

V

L

N

N

F

T

G

S

A

G

L

M

S

T

G

G

F

F

M

I

L

A

L

W

H

L

I

G

T

I

V

A

I

I

N

S

H

H

Y

Y

W

-

R

R

R

F

Q

R

R

R

S

G

G

Y

Q

V

L

L

I

Q

R

G

H

H

V

D

I

I

C

N

P

D

L

L

Y102 (≠ M100) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
W106 (≠ Y104) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
K163 (≠ T150) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
F216 (≠ L210) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
E222 (≠ D216) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
E230 (= E224) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
D275 (≠ K265) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
D278 (= D268) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
438 E→A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
443 D→A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
446 D→A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.

Q9QXW9 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; mLAT2; Solute carrier family 7 member 8 from Mus musculus (Mouse) (see paper)
22% identity, 78% coverage: 44:395/449 of query aligns to 66:419/531 of Q9QXW9

query
sites
Q9QXW9

E

E

A

N

P

A

G

G

M

S

V

V

P

G

L

L

A

A

Y

L

I

I

I

V

G

W

M

I

V

V

A

T

M

G

L

I

F

I

T

T

A

A

-

V

-

G

-

A

L

L

S

C

Y

Y

G

A

S

E

M

L

A

G

R

V

A

T

F

I

P

P

V

K

A

S

G

G

S

G

V

D

Y

Y

S

S

Y

Y

A

V

Q

K

R

D

G

I

L

F

N

G

P

G

H

L

V

A

G

G

F

F

I

L

A

R

G

L

W

W

L

I

M

A

L

V

L

L

D

-

Y

-

L

V

L

I

I
x
Y

P

P

P

T

-
x
N

-

Q

-

A

-

V

L

I

L

A

Y

L

V

T

Y

F

A

S

A

N

M

Y

A

V

L

L

N

Q

H

P

L

L

Y

F

P

P

D

T

I

C

-

F

-

P

P

P

K

E

V

S

G

G

F

L

I

R

L

L

A

L

F

A

L

A

V

I

S

C

A

L

-

L

T

T

F

W

V

V

N

N

L

C

R

S

G

S

I

V

T

R

F

W

T

A

A

T

R

R

M

V

N

Q

I

D

I

I

F

F

L

T

L

A

A

G

Q

K

L

L

V

L

V

A

L

L

G

A

I

L

F

I

L

I

F

I

Y

M

A

G

W

I

N

V

A

Q

L

I

H

C

G

K

G

G

A

E

G

-

H

-

G

-

Q

F

L

F

T

W

L

L

A

E

P

P

L

K

Y

N

S

A

P

F

E

E

H

N

F

F

-

Q

-

E

-

P

N

D

F

I

A

G

L

L

L

V

M

A

Q

L

A

A

V

F

S

L

I

Q

A

G

V

S

L
x
F

S

A

F

Y

L

G

G

G

F

W

D

N

A

F

I

L

S

N

T

Y

L

V

A

T

E

E

I

L

K

V

G

-

D

D

P

P

G

Y

R

K

S

N

V

L

G

P

K

R

A

A

A

I

L

F

V

I

T

S

L

I

L

P

V

L

M

V

G

T

A

F

I

V

F

Y

V

V

V

-

Q

-

T

-

W

-

I

-

A

-

T

-

D

-

L

-

A

-

G

-

M

-

G

-

F

-

K

-

S

-

A

-

D

-

T

-

A

-

F

F

Y

A

E

N

I

I

A

A

E

Y

L

V

A

T

A

A

G

M

S

S

W

P

L

Q

A

E

T

L

L

L

T

A

A

S

V

N

A

A

T

V

A

A

L

V

A

T

W

F

G

G

-

E

-

K

-

L

-

G

-

V

-

M

-

A

-

W

-

I

-

M

-

P

V

I

A

S

V

V

A

A

I

L

T

S

S

T

Q

F

A

G

V

V

-

N

-

G

-

S

-

L

-

F

-

T

-

S

S

S

R

R

L

L

L

F

F

F

G

A

M

G

A

A

R

R

D

E

G

G

Q

H

L

L

P

P

K

S

V

V

L

L

A

A

K

M

V

I

H

H

P

V

K

K

H

R

N

C

T

T

P

P

Y

I

M

P

S

A

I

L

Y

L

L

F

V

T

A

C

V

L

L

S

S

T

L

L

I

M

C

L

Y

-

L

-

F

-

I

-

N

-

A

V

V

T

D

S

T

D

L

M

T

Y

S

T

L

L

V

I

N

N

F

Y

G

V

A

G

L

F

S

I

G

N

F

Y

M

L

L

F

L

Y

H

G

I

V

T

T

V

V

I

A

N

G

H

Q

Y

I

W

V

R

L

R

R

Q

W

R

K

S

K

G

P

Q

D

L

I

I

P

R

R

H

P

V

I

Y130 (≠ I107) mutation to A: Increases T2 import. Increases T3 and enables T4 import. Does not affect L-leucine and L-phenylalanine uptake.
N133 (vs. gap) mutation to S: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake. Increases the export of both L-leucine and L-phenylalanine.
F242 (≠ L210) mutation to W: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake.

P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
23% identity, 73% coverage: 1:330/449 of query aligns to 1:328/469 of P46349

query
sites
P46349

M

M

S

N

I

Q

E

S

A

Q

F

S

G

G

Y

L

K

K

Q

K

E

E

L

L

K

K

-

T

R

R

G

H

L

M

S

T

L

M

T

I

D

S

L

I

-

A

-

G

V

V

V

I

Y

G

G

A

M

G

I

L

F

F

M

V

I

-

P

-

I

-

A

-

P

-

F

-

G
|
G

V

S

Y

G

G

S

Y

V

V

I

N

H

A

S

E

T

A
x
G

P

P

G

G

M

A

V

V

P

-

L

V

A

S

Y

Y

I

A

I

L

G

A

M

G

V

L

A

L

M

V

L

I

F

F

T

I

A

M

L

R

S

M

Y

L

G

G

S

E

M

M

A

S

R

A

A

V

F

N

P

P

V

T

A

S

G

G

S

S

V

F

Y

S

S

Q

Y

Y

A

A

Q

H

R

D

G

A

L

I

N

G

P

P

H

W

V

A

G

G

F

F

I

T

A

I

G

G

W

W

L

L

M

Y

L

W

L

F

D

F

Y

W

L

V

L

I

I

V

P

I

P

A

L

I

L

E

Y

A

V

I

Y

A

A

G

A

A

M

G

A

I

L

I

N

Q

H

Y

L

W

Y

F

P

H

D

D

I

I

P

P

K

L

V

W

G

L

F

T

I

S

L

L

A

I

F

L

L

T

V

I

S

V

A

L

T

T

F

L

V

T

N

N

L

V

R

Y

G

S

I

V

T

K

F

S

T

F

A

G

R

E

M

F

N

E

I

Y

I

W

F

F

L

S

L

L

A

I

Q

K

L

V

V

V

V

T

L

I

G

I

I

A

F

F

L

L

F

I

Y

V

A

G

W

F

N

A

A

F

L

I

H

F

G

G

G

F

A

A

-

P

-

G

-

S

-

E

-

P

-

V

G

G

H

F

G

S

Q

N

L

L

T

T

L

G

A

K

P

G

L

G

Y

F

S

F

P

P

E

E

H

G

F

I

N

S

F

S

A

V

L

L

M

-

Q

-

A

G

V

I

S

V

I

V

A

V

V

I

L

F

S

S

F

F

L

M

G

G

F

T

D

E

A

I

I

V

S

A

T

I

L

A

A

A

E

G

E

E

I

-

K

T

G

S

D

N

P

P

G

I

R

E

S

S

V

V

G

T

K

K

A

A

A

T

-

R

-

S

-

V

-

W

L

R

V

I

T

I

L

V

L

F

V

Y

M

V

G

G

A

S

I

I

F

A

V

I

V

V

Q

V

T

A

-

L

-

P

W

W

I

N

A

S

T

A

D

N

L

I

A

-

G

-

M

-

G

-

F

L

K

E

S

S

A

P

D

F

T

V

A

A

F

V

Y

L

E

E

I

H

A

I

E

G

L

V

A

P

A

A

G

A

S

A

W

Q

L

I

A

M

T

N

-

F

-

I

-

V

L

L

T

T

A

A

V

V

A

L

T

S

A

C

L

L

A

N

W

S

G
|
G

V

L

A

-

V

-

A

-

I

-

T

-

S

-

Q

-

A

Y

A

T

V

T

S

S

R

R

L

M

L

L

F

Y

G

S

M

L

A

A

R

E

D

R

G

N

Q

E

L

A

P

P

K

R

V

R

L

F

A

M

K

K

V

L

H

S

P

K

K

K

G33 (= G36) mutation to D: Lack of activity.
G42 (≠ A45) mutation to S: Lack of activity.
G301 (= G296) mutation to V: Lack of activity.

Sites not aligning to the query:

338 G→E: Lack of activity.
341 F→S: Lack of activity.
414 G→R: Lack of activity.

6s3kB Kima from bacillus subtilis in inward-facing, occluded state (see paper)
26% identity, 57% coverage: 66:323/449 of query aligns to 54:312/573 of 6s3kB

query
sites
6s3kB

S

S

Y

Y

G

R

S

Q

M

I

A

I

R

Y

A

A

F

Y

P

P

V

Q

A

G

G

G

S

G

V

A

Y

Y

S

I

Y

V

A

S

Q

K

R

E

G

N

L

L

N

G

P

E

H

K

V

P

G

G

F

L

I

I

A

A

G

G

W

G

L

S

M

L

L

L

L

V

D
|
D

Y
|
Y

L

I

L

L

I
x
T

P

V

P

A

L

V

L
x
S

Y

I

V

S

Y

A

A

G

A

T

M

D

A

A

L

I

N

T

H

S

L

A

Y

F

P

P

D

A

I

L

P

H

K

D

V

Y

G

H

F

V

I

P

L

I

A

A

-

I

F

F

L

L

V

V

S

L

A

V

T

I

F

M

V

I

-

L

N

N

L

L

R

R

G

G

I

L

T

S

F

E

T

S

A

A

R

S

M

I

-

L

-

A

-

Y

-

P

N

V

I

Y

I

L

F

F

L

V

A

A

Q

L

L

L

V

V

V

L

L

I

G

A

I

V

F

G

L

L

F

F

Y

K

A

L

W

M

N

T

A

G

L

Q

H

I

G

D

G

Q

A

P

G

A

H

H

G

H

Q

T

L

S

T

L

L

G

A

T

P

P

L

V

Y

A

S

G

P

I

E

T

H

L

F

F

N

-

F

-

A

-

L

L

M

L

Q

K

A

A

V

F

S

S

I

S

A

G

V

C

L

S

S

A

F

L

L
x
T

G

G

F

V

D
x
E

A
|
A

I

I

S

S

T

N

L

A

A

I

E

P

E

A

I

F

K

K

G

N

D

P

P

P

G

A

R

R

S

N

V

A

G

A

K

R

A

-

L

-

V

-

T

T

L

L

L

A

V

M

M

M

G

G

A

I

I

L

F

L

V

A

V

I

Q

L

T

F

W

S

I

G

A

I

T

T

D

V

L

L

A

A

A

Y

G

G

M

Y

G

G

F

T

-

A

-

P

K

K

S

P

A

D

D

E

T

T

A

V

F

V

Y

S

E

Q

I

I

A

A

E

S

L

E

A

T

A

F

G

G

S

R

W

N

L

V

A

F

T

Y

L

Y

T

V

A

I

V

Q

A

G

T

V

A

T

L

S

A

L

W

I

G

L

V

V

A

L

V
x
A

A

A

I

N

T

T

S

G

Q

F

A

S

A

A

V

F

S

P

R

Q

L

L

L

A

F

F

G

N

M

L

A

A

R

R

D

D

G

Q

Q

Y

L

M

P

P

K

R

V

M

binding potassium ion: D91 (= D103), Y92 (= Y104), T95 (≠ I107), S99 (≠ L111), T204 (≠ L213), E207 (≠ D216), A208 (= A217), A288 (≠ V299)

Sites not aligning to the query:

binding potassium ion: 10, 10, 10, 13, 17

P96589 Potassium transporter KimA; K(+) importer A; Potassium-proton symporter KimA from Bacillus subtilis (strain 168) (see paper)
26% identity, 57% coverage: 66:323/449 of query aligns to 80:338/607 of P96589

query
sites
P96589

S

S

Y

Y

G

R

S

Q

M

I

A

I

R

Y

A

A

F

Y

P

P

V

Q

A

G

G

G

S

G

V

A

Y

Y

S

I

Y

V

A

S

Q

K

R

E

G

N

L

L

N

G

P

E

H

K

V

P

G

G

F

L

I

I

A

A

G

G

W

G

L

S

M

L

L

L

L

V

D
|
D

Y

Y

L

I

L

L

I
x
T

P

V

P

A

L

V

L
x
S

Y

I

V

S

Y

A

A

G

A

T

M

D

A

A

L

I

N

T

H

S

L

A

Y

F

P

P

D

A

I

L

P

H

K

D

V

Y

G

H

F

V

I

P

L

I

A

A

-

I

F

F

L

L

V

V

S

L

A

V

T

I

F

M

V

I

-

L

N

N

L

L

R

R

G

G

I

L

T

S

F

E

T

S

A

A

R

S

M

I

-

L

-

A

-

Y

-

P

N

V

I

Y

I

L

F

F

L

V

A

A

Q

L

L

L

V

V

V

L

L

I

G

A

I

V

F

G

L

L

F

F

Y

K

A

L

W

M

N

T

A

G

L

Q

H

I

G

D

G

Q

A

P

G

A

H

H

G

H

Q

T

L

S

T

L

L

G

A

T

P

P

L

V

Y

A

S

G

P

I

E

T

H

L

F

F

N

-

F

-

A

-

L

L

M

L

Q

K

A

A

V

F

S

S

I

S

A

G

V

C

L

S

S

A

F

L

L

T

G

G

F

V

D
x
E

A

A

I

I

S

S

T

N

L

A

A

I

E

P

E

A

I

F

K

K

G

N

D

P

P

P

G

A

R

R

S

N

V

A

G

A

K

R

A

-

L

-

V

-

T

T

L

L

L

A

V

M

M

M

G

G

A

I

I

L

F

L

V

A

V

I

Q

L

T

F

W

S

I

G

A

I

T

T

D

V

L

L

A

A

A

Y

G

G

M

Y

G

G

F

T

-

A

-

P

K

K

S

P

A

D

D

E

T

T

A

V

F

V

Y

S

E

Q

I

I

A

A

E

S

L

E

A

T

A

F

G

G

S

R

W

N

L

V

A

F

T

Y

L

Y

T

V

A

I

V

Q

A

G

T

V

A

T

L

S

A

L

W

I

G

L

V

V

A

L

V

A

A

A

I

N

T

T

S

G

Q

F

A

S

A

A

V

F

S

P

R

Q

L

L

L

A

F

F

G

N

M

L

A

A

R

R

D

D

G

Q

Q

Y

L

M

P

P

K

R

V

M

D117 (= D103) binding ; mutation D->A,N,E: No effect on growth.
T121 (≠ I107) mutation to A: Decreases potassium uptake.
S125 (≠ L111) binding ; mutation to A: Decreases potassium uptake.
E233 (≠ D216) mutation E->A,Q: Abolishes potassium uptake.

Sites not aligning to the query:

36 binding ; binding ; mutation D->A,N: Abolishes potassium uptake.
43 binding ; Y→N: Abolishes potassium uptake.
377 Y→A: Decreases potassium uptake.

Q01650 Large neutral amino acids transporter small subunit 1; 4F2 light chain; 4F2 LC; 4F2LC; CD98 light chain; Integral membrane protein E16; E16; L-type amino acid transporter 1; hLAT1; Solute carrier family 7 member 5; y+ system cationic amino acid transporter from Homo sapiens (Human) (see 3 papers)
30% identity, 20% coverage: 308:395/449 of query aligns to 347:429/507 of Q01650

query
sites
Q01650

S

S

R

R

L

L

L

F

F

F

G

V

M

G

A

S

R

R

D

E

G

G

Q

H

L

L

P

P

K

S

V

I

L

L

A

S

K

M

V

I

H

H

P

P

K

Q

H

L

N

L

T

T

P

P

Y

V

M
x
P

S

S

I

L

Y

V

L

F

V

T

A

C

V

V

L

M

S

T

L

L

I

Y

C

A

Y

F

L

-

F

-

I

-

N

-

A

-

V

S

D

K

T

D

L

I

T

F

S

S

L

V

V

I

N

N

F

F

G

F

A

S

L

F

S

F

G

N

F

W

M

L

L

C

L

V

H

A

I

L

T

A

V

I

I

I

N

G

H

M

Y

I

W

W

R

L

R

R

Q

H

R

R

S

K

G

P

Q

E

L

L

I

E

R

R

H

P

V

I

P375 (≠ M336) mutation to L: Nearly abolishes leucine transport activity.

Sites not aligning to the query:

117 Y→A: Strongly decreased leucine transport activity.
164 modified: Interchain (with C-210 in SLC3A2)
223 D → V: in dbSNP:rs17853937
230 N → K: in dbSNP:rs1060250
246 A→V: Nearly abolishes leucine transport activity.
252 F→A: Nearly abolishes leucine transport activity.
257 W→A: Nearly abolishes leucine transport activity.
258 N→A: Decreased leucine transport activity.; N→D: Nearly abolishes leucine transport activity.
259 Y→A: Strongly decreased leucine transport activity.
303 E→K: Decreased leucine transport activity.
483:507 mutation Missing: Nearly abolishes leucine transport activity.

6irtB Human lat1-4f2hc complex bound with bch (see paper)
30% identity, 20% coverage: 308:395/449 of query aligns to 297:379/457 of 6irtB

query
sites
6irtB

S

S

R

R

L

L

L

F

F

F

G

V

M

G

A

S

R

R

D

E

G

G

Q

H

L

L

P

P

K

S

V

I

L

L

A

S

K

M

V

I

H

H

P

P

K

Q

H

L

N

L

T

T

P

P

Y

V

M

P

S

S

I

L

Y

V

L

F

V

T

A

C

V

V

L

M

S

T

L

L

I

Y

C

A

Y

F

L

-

F

-

I

-

N

-

A

-

V

S

D

K

T

D

L

I

T

F

S

S

L

V

V

I

N

N

F

F

G

F

A

S

L

F

S

F

G

N

F

W

M

L

L

C

L

V

H

A

I

L

T

A

V

I

I

I

N

G

H

M

Y

I

W

W

R

L

R

R

Q

H

R

R

S

K

G

P

Q

E

L

L

I

E

R

R

H

P

V

I

Sites not aligning to the query:

binding (1S,2S,4R)-2-aminobicyclo[2.2.1]heptane-2-carboxylic acid: 15, 17, 94, 202, 205

7dsqB Overall structure of the lat1-4f2hc bound with 3,5-diiodo-l-tyrosine (see paper)
30% identity, 20% coverage: 308:395/449 of query aligns to 304:386/464 of 7dsqB

query
sites
7dsqB

S

S

R

R

L

L

L

F

F

F

G

V

M

G

A

S

R

R

D

E

G

G

Q

H

L

L

P

P

K

S

V

I

L

L

A

S

K

M

V

I

H

H

P

P

K

Q

H

L

N

L

T

T

P

P

Y

V

M

P

S

S

I

L

Y

V

L

F

V

T

A

C

V

V

L

M

S

T

L

L

I

Y

C

A

Y

F

L

-

F

-

I

-

N

-

A

-

V

S

D

K

T

D

L

I

T

F

S

S

L

V

V

I

N

N

F

F

G
x
F

A

S

L

F

S

F

G
x
N

F

W

M

L

L

C

L

V

H

A

I

L

T

A

V

I

I

I

N

G

H

M

Y

I

W

W

R

L

R

R

Q

H

R

R

S

K

G

P

Q

E

L

L

I

E

R

R

H

P

V

I

binding 3,5-diiodotyrosine: F357 (≠ G366), N361 (≠ G370)

Sites not aligning to the query:

binding 3,5-diiodotyrosine: 19, 20, 22, 212, 213, 216
binding cholesterol hemisuccinate: 148, 153

Query Sequence

>AO356_01520 FitnessBrowser__pseudo5_N2C3_1:AO356_01520
MSIEAFGYKQELKRGLSLTDLVVYGMIFMIPIAPFGVYGYVNAEAPGMVPLAYIIGMVAM
LFTALSYGSMARAFPVAGSVYSYAQRGLNPHVGFIAGWLMLLDYLLIPPLLYVYAAMALN
HLYPDIPKVGFILAFLVSATFVNLRGITFTARMNIIFLLAQLVVLGIFLFYAWNALHGGA
GHGQLTLAPLYSPEHFNFALLMQAVSIAVLSFLGFDAISTLAEEIKGDPGRSVGKAALVT
LLVMGAIFVVQTWIATDLAAGMGFKSADTAFYEIAELAAGSWLATLTAVATALAWGVAVA
ITSQAAVSRLLFGMARDGQLPKVLAKVHPKHNTPYMSIYLVAVLSLLICYLFINAVDTLT
SLVNFGALSGFMLLHITVINHYWRRQRSGQLIRHVICPLVGFVIVAAIMYNMGVAAQKLG
LIWIAAGVVYLCLLNKFGSRTALPDPAGQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory