SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO356_04410 FitnessBrowser__pseudo5_N2C3_1:AO356_04410 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5vg6B Crystal structure of d-isomer specific 2-hydroxyacid dehydrogenase from xanthobacter autotrophicus py2 in complex with NADPH and mes.
32% identity, 78% coverage: 70:310/310 of query aligns to 74:315/315 of 5vg6B

query
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5vg6B

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active site: M98 (≠ F93), R230 (= R225), D254 (= D249), E259 (= E254), H278 (= H273)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A74 (= A70), R92 (= R88), M102 (= M97), L147 (≠ T142), G148 (= G143), D149 (= D144), L150 (≠ I145), W168 (≠ I163), S169 (≠ A164), R170 (≠ S165), T171 (≠ E166), K173 (≠ R168), L201 (= L196), P202 (= P197), T207 (= T202), V228 (= V223), R230 (= R225), H278 (= H273), A280 (≠ S275), S281 (≠ A276), Y315 (= Y310)

5mh6A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-ketohexanoic acid and NAD+ (1.35 a resolution)
32% identity, 71% coverage: 86:304/310 of query aligns to 82:305/306 of 5mh6A

query
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binding 2-Ketohexanoic acid: H89 (≠ F93), R224 (= R225), H272 (= H273), Y280 (vs. gap)
binding magnesium ion: T130 (≠ G131), A132 (= A133), F210 (= F211), E211 (≠ K212), M213 (≠ F214), G225 (= G226), P226 (≠ V227), V228 (= V229), E230 (≠ D231), D241 (≠ H242), S251 (≠ R252)
binding nicotinamide-adenine-dinucleotide: T86 (≠ V90), H89 (≠ F93), G142 (= G143), T143 (≠ D144), L144 (≠ I145), R164 (≠ S165), P196 (= P197), T201 (= T202), V222 (= V223), A223 (≠ G224), R224 (= R225), H272 (= H273), S274 (= S275)

Sites not aligning to the query:

5mhaB D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
32% identity, 71% coverage: 86:304/310 of query aligns to 84:307/308 of 5mhaB

query
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5mhaB

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binding 2-Ketohexanoic acid: R226 (= R225), H274 (= H273), Y282 (vs. gap)
binding (2R)-2-hydroxyhexanoic acid: H91 (≠ F93), Y282 (vs. gap)
binding magnesium ion: F212 (= F211), E213 (≠ K212), M215 (≠ F214), D243 (≠ H242)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T88 (≠ V90), L143 (≠ T142), G144 (= G143), T145 (≠ D144), L146 (≠ I145), R165 (≠ A164), R166 (≠ S165), S167 (≠ E166), P180 (≠ L179), T197 (≠ L196), P198 (= P197), T203 (= T202), V224 (= V223), A225 (≠ G224), R226 (= R225), H274 (= H273), S276 (= S275)

Sites not aligning to the query:

5mhaA D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
32% identity, 71% coverage: 86:304/310 of query aligns to 84:307/308 of 5mhaA

query
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5mhaA

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binding 2-Ketohexanoic acid: H91 (≠ F93), R226 (= R225), H274 (= H273), Y282 (vs. gap)
binding magnesium ion: T132 (≠ G131), A134 (= A133)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T88 (≠ V90), L143 (≠ T142), G144 (= G143), T145 (≠ D144), L146 (≠ I145), R165 (≠ A164), R166 (≠ S165), S167 (≠ E166), P180 (≠ L179), T197 (≠ L196), P198 (= P197), T203 (= T202), V224 (= V223), A225 (≠ G224), R226 (= R225), H274 (= H273), S276 (= S275)

Sites not aligning to the query:

5mh5A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-keto-hexanoic acid and NADP+ (1.4 a resolution)
32% identity, 71% coverage: 86:304/310 of query aligns to 84:307/308 of 5mh5A

query
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5mh5A

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E

A

S

D

D

L

L

V

V

Q

A

A

A

L

L

K

D

D

S

G

G

H
x
D

L

I

A

A

G

G

A

A

V

A

I

L

D

D

V

V

C

F

R

S

Q

E

E

E

P

P

L

L

P

P

Q

E

R

D

H

S

P

P

F

L

W

W

T

D

A

F

W

E

G

D

L

V

L

L

L

I

T

T

G

P

H
|
H

S

V

S
|
S

A

A

P

A

T

T

S

S

P

K

-
x
Y

-

H

P

E

M

D

M

V

V

A

Q

A

L

L

F

I

L

R

E

E

N

N

L

I

R

E

A

K

Y

I

Q

A

A

T

G

G

E

D

A

E

L

L

R

T

G

N

E

R

V

V

binding 2-Ketohexanoic acid: H91 (≠ F93), R226 (= R225), H274 (= H273), Y282 (vs. gap)
binding magnesium ion: T132 (≠ G131), A134 (= A133), F212 (= F211), E213 (≠ K212), M215 (≠ F214), D243 (≠ H242)
binding nadp nicotinamide-adenine-dinucleotide phosphate: T88 (≠ V90), G142 (= G141), L143 (≠ T142), G144 (= G143), T145 (≠ D144), L146 (≠ I145), R165 (≠ A164), R166 (≠ S165), S167 (≠ E166), T197 (≠ L196), P198 (= P197), T203 (= T202), V224 (= V223), A225 (≠ G224), R226 (= R225), H274 (= H273), S276 (= S275)

Sites not aligning to the query:

7jqiA Structure of wild type glyoxylate/hydroxypyruvate reductase a from escherichia coli in complex with alpha-ketoglutarate and NADP+
28% identity, 78% coverage: 70:310/310 of query aligns to 65:312/312 of 7jqiA

query
sites
7jqiA

A
|
A

G
|
G

I

V

T

D

P

S

L

I

L

L

A

S

D

K

G

L

L

Q

S

A

R

H

D

P

Y

E

R

M

L

L

T

N

R

P

A

S

V

V

G

P

I

L

F

F

-
x
R

-

L

-

E

-

D

-

T

-

G

-

M

G

G

Q

E

V

Q

M
|
M

A

Q

E

E

Y

Y

V

A

L

V

T

S

Y

Q

M

V

L

L

G

H

H

W

E

F

R

R

E

R

V

F

L

D

A

D

R

Y

L

R

V

I

S

Q

Q

Q

V

N

E

S

R

S

K

H

W

W

D

Q

N

P

R

L

Q

P

G

E

Q

Y

G

H

L

R

A

E

G

D

R

F

K

T

V

I

L

G

I

I

V

L

G

G

T
x
A

G
|
G

D
x
V

I
x
L

G

G

Q

S

R

K

V

V

A

A

Q

Q

F

S

L

L

M

Q

P

T

F

W

G

R

V

F

Q

P

L

L

Y

R

G

C

I
x
W

A
x
S

S
x
R

E
x
T

A

R

R
x
K

T

S

L

W

A

P

P

G

F

V

I

Q

E

S

V

F

G

A

A

G

L

R

K

E

D

E

L

L

P

S

R

A

L

F

V

L

G

S

D

Q

M

C

D

R

Y

V

V

L

I

I

N

N

L

L

L
|
L

P
|
P

N

N

T

T

P

P

D

E

T

T

H

V

D

G

I

I

Y

I

D

N

A

Q

L

L

F

L

K

E

Q

K

F

L

K

P

P

D

S

G

G

A

L

Y

F

L

I

L

N

N

V
x
L

G

A

R
|
R

G

G

V

V

A

H

V

V

D

E

A

D

D

D

L

L

V

L

Q

A

A

A

L

L

K

D

D

S

G

G

H

K

L

V

A

K

G

G

A

A

V

M

I

L

D

D

V

V

C

F

R

N

Q

R

E

E

P

P

L

L

P

P

Q

P

R

E

H

S

P

P

F

L

W

W

T

Q

A

H

W

P

G

R

L

V

L

T

L

I

T

T

G

P

H
|
H

S

V

S

A

A
|
A

P

I

T

T

S

R

P

P

P

A

M

E

M

A

V

V

Q

E

L

Y

F

I

L

S

E

R

N

T

L

I

R

A

A

Q

Y

L

Q

E

A

K

G

G

E

E

A

K

L

V

R

C

G

G

E

Q

V

V

D

D

F

R

S

A

R

R

G

G

Y
|
Y

binding 2-oxoglutaric acid: A65 (= A70), G66 (= G71), R227 (= R225), H275 (= H273)
binding nadp nicotinamide-adenine-dinucleotide phosphate: A65 (= A70), R89 (vs. gap), M99 (= M97), A144 (≠ T142), G145 (= G143), V146 (≠ D144), L147 (≠ I145), W165 (≠ I163), S166 (≠ A164), R167 (≠ S165), T168 (≠ E166), K170 (≠ R168), L198 (= L196), P199 (= P197), L225 (≠ V223), R227 (= R225), H275 (= H273), A278 (= A276), Y312 (= Y310)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 64

6p35A 2.5 angstrom structure of wild type glyoxylate/hydroxypyruvate reductase a from escherichia coli in complex with 2-keto arginine and NADP
28% identity, 78% coverage: 70:310/310 of query aligns to 65:312/312 of 6p35A

query
sites
6p35A

A
|
A

G

G

I

V

T

D

P

S

L

I

L

L

A

S

D

K

G

L

L

Q

S

A

R

H

D

P

Y

E

R

M

L

L

T

N

R

P

A

S

V

V

G

P

I

L

F

F

-
x
R

-

L

-

E

-

D

-

T

-

G

-

M

G

G

Q

E

V

Q

M
|
M

A

Q

E

E

Y

Y

V

A

L

V

T

S

Y

Q

M

V

L

L

G

H

H

W

E

F

R

R

E

R

V

F

L

D

A

D

R

Y

L

R

V

I

S

Q

Q

Q

V

N

E

S

R

S

K

H

W

W

D

Q

N

P

R

L

Q

P

G

E

Q

Y

G

H

L

R

A

E

G

D

R

F

K

T

V

I

L

G

I

I

V

L

G

G

T
x
A

G
|
G

D
x
V

I
x
L

G

G

Q

S

R

K

V

V

A

A

Q

Q

F

S

L

L

M

Q

P

T

F

W

G

R

V

F

Q

P

L

L

Y

R

G

C

I
x
W

A
x
S

S
x
R

E
x
T

A

R

R
x
K

T

S

L

W

A

P

P

G

F

V

I

Q

E

S

V

F

G

A

A

G

L

R

K

E

D

E

L

L

P

S

R

A

L

F

V

L

G

S

D

Q

M

C

D

R

Y

V

V

L

I

I

N

N

L

L

L
|
L

P
|
P

N

N

T

T

P

P

D

E

T

T

H

V

D

G

I

I

Y

I

D

N

A

Q

L

L

F

L

K

E

Q

K

F

L

K

P

P

D

S

G

G

A

L

Y

F

L

I

L

N

N

V
x
L

G

A

R
|
R

G

G

V

V

A

H

V

V

D

E

A

D

D

D

L

L

V

L

Q

A

A

A

L

L

K

D

D

S

G

G

H

K

L

V

A

K

G

G

A

A

V

M

I

L

D

D

V

V

C

F

R

N

Q

R

E

E

P

P

L

L

P

P

Q

P

R

E

H

S

P

P

F

L

W

W

T

Q

A

H

W

P

G

R

L

V

L

T

L

I

T

T

G

P

H
|
H

S

V

S
x
A

A
|
A

P

I

T
|
T

S

R

P

P

P

A

M

E

M

A

V

V

Q

E

L

Y

F

I

L

S

E

R

N

T

L

I

R

A

A

Q

Y

L

Q

E

A

K

G

G

E

E

A

K

L

V

R

C

G

G

E

Q

V

V

D

D

F

R

S

A

R

R

G

G

Y
|
Y

binding nadp nicotinamide-adenine-dinucleotide phosphate: A65 (= A70), R89 (vs. gap), M99 (= M97), A144 (≠ T142), G145 (= G143), V146 (≠ D144), L147 (≠ I145), W165 (≠ I163), S166 (≠ A164), R167 (≠ S165), T168 (≠ E166), K170 (≠ R168), L198 (= L196), P199 (= P197), L225 (≠ V223), R227 (= R225), H275 (= H273), A277 (≠ S275), A278 (= A276), Y312 (= Y310)
binding 5-[(diaminomethylidene)amino]-2-oxopentanoic acid: A65 (= A70), R227 (= R225), H275 (= H273), T280 (= T278)

Sites not aligning to the query:

binding 5-[(diaminomethylidene)amino]-2-oxopentanoic acid: 10, 11, 15

7jqhA Structure of wild type glyoxylate/hydroxypyruvate reductase a from escherichia coli in complex with phosphate and NADP+
28% identity, 78% coverage: 70:310/310 of query aligns to 66:313/313 of 7jqhA

query
sites
7jqhA

A
|
A

G

G

I

V

T

D

P

S

L

I

L

L

A

S

D

K

G

L

L

Q

S

A

R

H

D

P

Y

E

R

M

L

L

T

N

R

P

A

S

V

V

G

P

I

L

F

F

-
x
R

-

L

-

E

-

D

-

T

-

G

-

M

G

G

Q

E

V

Q

M
|
M

A

Q

E

E

Y

Y

V

A

L

V

T

S

Y

Q

M

V

L

L

G

H

H

W

E

F

R

R

E

R

V

F

L

D

A

D

R

Y

L

R

V

I

S

Q

Q

Q

V

N

E

S

R

S

K

H

W

W

D

Q

N

P

R

L

Q

P

G

E

Q

Y

G

H

L

R

A

E

G

D

R

F

K

T

V

I

L

G

I

I

V

L

G

G

T
x
A

G
|
G

D
x
V

I
x
L

G

G

Q

S

R

K

V

V

A

A

Q

Q

F

S

L

L

M

Q

P

T

F

W

G

R

V

F

Q

P

L

L

Y

R

G

C

I
x
W

A
x
S

S
x
R

E
x
T

A

R

R
x
K

T

S

L

W

A

P

P

G

F

V

I

Q

E

S

V

F

G

A

A

G

L

R

K

E

D

E

L

L

P

S

R

A

L

F

V

L

G

S

D

Q

M

C

D

R

Y

V

V

L

I

I

N

N

L

L

L
|
L

P
|
P

N

N

T

T

P

P

D

E

T

T

H

V

D

G

I

I

Y

I

D

N

A

Q

L

L

F

L

K

E

Q

K

F

L

K

P

P

D

S

G

G

A

L

Y

F

L

I

L

N

N

V
x
L

G
x
A

R
|
R

G

G

V

V

A

H

V

V

D

E

A

D

D

D

L

L

V

L

Q

A

A

A

L

L

K

D

D

S

G

G

H

K

L

V

A

K

G

G

A

A

V

M

I

L

D
|
D

V

V

C

F

R

N

Q

R

E

E

P

P

L

L

P

P

Q

P

R

E

H

S

P

P

F

L

W

W

T

Q

A

H

W

P

G

R

L

V

L

T

L

I

T

T

G

P

H
|
H

S

V

S

A

A
|
A

P

I

T

T

S

R

P

P

P

A

M

E

M

A

V

V

Q

E

L

Y

F

I

L

S

E

R

N

T

L

I

R

A

A

Q

Y

L

Q

E

A

K

G

G

E

E

A

K

L

V

R

C

G

G

E

Q

V

V

D

D

F

R

S

A

R

R

G

G

Y
|
Y

binding nadp nicotinamide-adenine-dinucleotide phosphate: A66 (= A70), R90 (vs. gap), M100 (= M97), A145 (≠ T142), G146 (= G143), V147 (≠ D144), L148 (≠ I145), W166 (≠ I163), S167 (≠ A164), R168 (≠ S165), T169 (≠ E166), K171 (≠ R168), L199 (= L196), P200 (= P197), L226 (≠ V223), A227 (≠ G224), R228 (= R225), D252 (= D249), H276 (= H273), A279 (= A276), Y313 (= Y310)

5tsdA Crystal structure of NADPH-dependent 2-hydroxyacid dehydrogenase from rhizobium etli cfn 42 in complex with NADPH and oxalate
27% identity, 95% coverage: 15:310/310 of query aligns to 17:316/316 of 5tsdA

query
sites
5tsdA

Q

R

L

V

L

L

R

K

Q

T

A

A

A

F

P

A

D

D

I

R

E

G

V

S

L

I

T

N

S

L

G

A

D

D

S

P

A

A

E

N

L

R

S

E

R

R

L

D

A

F

T

S

D

E

C

T

P

E

-

Y

V

A

W

L

L

L

G
x
W

Q

K

P

P

D

D

L

-

A

A

T

D

L

L

L

F

R

R

Q

R

G

A

H

P

Q

N

P

L

R

K

W

V

L

I

Q

F

S

S

T

G

W
x
G

A
|
A

G
|
G

I

V

T

D

P

H

L

I

A

G

D

M

G

A

L

G

S

L

R

P

D

D

Y

I

R

P

L

I

T

V

R
|
R

A

F

V

V

G

D

-

R

I

S

F

L

G

T

Q

T

V

R

M
|
M

A

S

E

E

Y

W

V

V

L

V

T

M

Y

Q

M

C

L

L

G

M

H

H

E

L

R

R

E

G

V

Q

L

Y

A

G

R

H

L

D

V

S

S

H

Q

Q

V

R

E

R

R

R

K

E

W

W

D

A

N

K

R

L

Q

I

G

A

Q

P

G

E

L

A

A

A

G

E

R

V

K

T

V

V

L

G

I

V

V

M

G
|
G

T
x
L

G
|
G

D
x
I

I
x
L

G

G

Q

Q

R

D

V

A

A

V

Q

A

F

K

L

L

M

K

P

V

F

M

G

G

V

F

Q

N

L

V

Y

I

G

G

I
x
W

A
x
S

S
x
R

E
x
T

A

R

R
x
K

T

T

L

I

A

E

P

G

F

-

I

V

E

E

V

T

G

F

A

D

L

A

K

G

D

E

L

L

P

D

R

R

L

F

V

L

G

A

D

K

M

T

D

D

Y

I

V

L

I

V

N

G

L

L

L
|
L

P
|
P

N

L

T

T

P

P

D

E

T

T

H

T

D

G

I

F

Y

Y

D

D

A

S

A

E

L

L

F

F

K

K

Q

K

F

L

K

R

P

R

S

D

G

G

-

A

-

L

-

G

-

Q

-

P

L

V

F

F

I

I

N

N

V
x
A

G
|
G

R
|
R

G

G

V

K

A

S

V

Q

V

I

D

E

A

T

D

D

L

I

V

V

Q

S

A

A

L

V

K

R

D

E

G

G

H

T

L

L

A

G

G

G

A

A

V

S

I

L

D
|
D

V

V

C

F

R

E

Q

V

E
|
E

P

P

L

L

P

A

Q

T

R

D

H

S

P

P

F

L

W

W

T

E

A

L

W

E

G

N

L

V

L

F

L

I

T

T

G

P

H
|
H

S

D

S

A

A

A

P

V

T

S

S

E

P

E

P

N

M

A

M

L

V

F

Q

R

L

H

F

V

L

E

E

M

N

Q

L

I

R

A

A

R

Y

F

Q

E

A

R

G

G

E

E

A

P

L

L

R

Q

G

F

E

V

V

I

D

D

F

R

S

A

R

A

G

G

Y
|
Y

active site: E260 (= E254), H279 (= H273)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A71 (= A70), R89 (= R88), M99 (= M97), G143 (= G141), L144 (≠ T142), G145 (= G143), I146 (≠ D144), L147 (≠ I145), W165 (≠ I163), S166 (≠ A164), R167 (≠ S165), T168 (≠ E166), K170 (≠ R168), L197 (= L196), P198 (= P197), A229 (≠ V223), G230 (= G224), R231 (= R225), D255 (= D249), H279 (= H273), Y316 (= Y310)
binding oxalic acid: W50 (≠ G47), G70 (≠ W69), A71 (= A70), G72 (= G71), R231 (= R225), H279 (= H273)

5bqfA Probable 2-hydroxyacid dehydrogenase from rhizobium etli cfn 42 in complex with NADP, hepes and l(+)-tartaric acid
27% identity, 95% coverage: 15:310/310 of query aligns to 18:317/317 of 5bqfA

query
sites
5bqfA

Q

R

L

V

L

L

R

K

Q

T

A

A

A

F

P

A

D

D

I

R

E

G

V

S

L

I

T

N

S

L

G

A

D

D

S

P

A

A

E

N

L

R

S

E

R

R

L

D

A

F

T

S

D

E

C

T

P

E

-

Y

V

A

W

L

L

L

G

W

Q

K

P

P

D

D

L

-

A

A

T

D

L

L

L

F

R

R

Q

R

G

A

H

P

Q

N

P

L

R

K

W

V

L

I

Q

F

S

S

T

G

W

G

A
|
A

G

G

I

V

T

D

P

H

L

I

A

G

D

M

G

A

L

G

S

L

R

P

D

D

Y

I

R

P

L

I

T

V

R
|
R

A

F

V

V

G

D

-

R

I

S

F

L

G

T

Q

T

V

R

M
|
M

A

S

E

E

Y

W

V

V

L

V

T

M

Y

Q

M

C

L

L

G

M

H

H

E

L

R

R

E

G

V

Q

L

Y

A

G

R

H

L

D

V

S

S

H

Q

Q

V

R

E

R

R

R

K

E

W

W

D

A

N

K

R

L

Q

I

G

A

Q

P

G

E

L

A

A

A

G

E

R

V

K

T

V

V

L

G

I

V

V

M

G
|
G

T
x
L

G
|
G

D
x
I

I
x
L

G

G

Q

Q

R

D

V

A

A

V

Q

A

F

K

L

L

M

K

P

V

F

M

G

G

V

F

Q

N

L

V

Y

I

G

G

I
x
W

A
x
S

S
x
R

E
x
T

A

R

R

K

T

T

L

I

A

E

P

G

F

-

I

V

E

E

V

T

G

F

A

D

L

A

K

G

D

E

L

L

P

D

R

R

L

F

V

L

G

A

D

K

M

T

D

D

Y

I

V

L

I

V

N

G

L

L

L
|
L

P
|
P

N

L

T

T

P

P

D

E

T

T

H

T

D

G

I

F

Y

Y

D

D

A

S

A

E

L

L

F

F

K

K

Q

K

F

L

K

R

P

R

S

D

G

G

-

A

-

L

-

G

-

Q

-

P

L

V

F

F

I

I

N

N

V
x
A

G
|
G

R
|
R

G

G

V

K

A

S

V

Q

V

I

D

E

A

T

D

D

L

I

V

V

Q

S

A

A

L

V

K

R

D

E

G

G

H

T

L

L

A

G

G

G

A

A

V

S

I

L

D

D

V

V

C

F

R

E

Q

V

E
|
E

P

P

L

L

P

A

Q

T

R

D

H

S

P

P

F

L

W

W

T

E

A

L

W

E

G

N

L

V

L

F

L

I

T

T

G

P

H
|
H

S

D

S

A

A
|
A

P

V

T

S

S

E

P

E

P

N

M

A

M

L

V

F

Q

R

L

H

F

V

L

E

E

M

N

Q

L

I

R

A

A

R

Y

F

Q

E

A

R

G

G

E

E

A

P

L

L

R

Q

G

F

E

V

V

I

D

D

F

R

S

A

R

A

G

G

Y
|
Y

active site: E261 (= E254), H280 (= H273)
binding nadp nicotinamide-adenine-dinucleotide phosphate: A72 (= A70), R90 (= R88), M100 (= M97), G144 (= G141), L145 (≠ T142), G146 (= G143), I147 (≠ D144), L148 (≠ I145), W166 (≠ I163), S167 (≠ A164), R168 (≠ S165), T169 (≠ E166), L198 (= L196), P199 (= P197), A230 (≠ V223), G231 (= G224), R232 (= R225), H280 (= H273), A283 (= A276), Y317 (= Y310)

4xcvA Probable 2-hydroxyacid dehydrogenase from rhizobium etli cfn 42 in complex with NADPH
27% identity, 95% coverage: 15:310/310 of query aligns to 18:317/317 of 4xcvA

query
sites
4xcvA

Q

R

L

V

L

L

R

K

Q

T

A

A

A

F

P

A

D

D

I

R

E

G

V

S

L

I

T

N

S

L

G

A

D

D

S

P

A

A

E

N

L

R

S

E

R

R

L

D

A

F

T

S

D

E

C

T

P

E

-

Y

V

A

W

L

L

L

G

W

Q

K

P

P

D

D

L

-

A

A

T

D

L

L

L

F

R

R

Q

R

G

A

H

P

Q

N

P

L

R

K

W

V

L

I

Q

F

S

S

T

G

W

G

A
|
A

G

G

I

V

T

D

P

H

L

I

A

G

D

M

G

A

L

G

S

L

R

P

D

D

Y

I

R

P

L

I

T

V

R
|
R

A

F

V

V

G

D

-

R

I

S

F

L

G

T

Q

T

V

R

M
|
M

A

S

E

E

Y

W

V

V

L

V

T

M

Y

Q

M

C

L

L

G

M

H

H

E

L

R

R

E

G

V

Q

L

Y

A

G

R

H

L

D

V

S

S

H

Q

Q

V

R

E

R

R

R

K

E

W

W

D

A

N

K

R

L

Q

I

G

A

Q

P

G

E

L

A

A

A

G

E

R

V

K

T

V

V

L

G

I

V

V

M

G
|
G

T
x
L

G
|
G

D
x
I

I
x
L

G

G

Q

Q

R

D

V

A

A

V

Q

A

F

K

L

L

M

K

P

V

F

M

G

G

V

F

Q

N

L

V

Y

I

G

G

I
x
W

A
x
S

S
x
R

E
x
T

A

R

R
x
K

T

T

L

I

A

E

P

G

F

-

I

V

E

E

V

T

G

F

A

D

L

A

K

G

D

E

L

L

P

D

R

R

L

F

V

L

G

A

D

K

M

T

D

D

Y

I

V

L

I

V

N

G

L

L

L
|
L

P
|
P

N

L

T

T

P

P

D

E

T

T

H

T

D

G

I

F

Y

Y

D

D

A

S

A

E

L

L

F

F

K

K

Q

K

F

L

K

R

P

R

S

D

G

G

-

A

-

L

-

G

-

Q

-

P

L

V

F

F

I

I

N

N

V
x
A

G
|
G

R
|
R

G

G

V

K

A

S

V

Q

V

I

D

E

A

T

D

D

L

I

V

V

Q

S

A

A

L

V

K

R

D

E

G

G

H

T

L

L

A

G

G

G

A

A

V

S

I

L

D

D

V

V

C

F

R

E

Q

V

E
|
E

P

P

L

L

P

A

Q

T

R

D

H

S

P

P

F

L

W

W

T

E

A

L

W

E

G

N

L

V

L

F

L

I

T

T

G

P

H
|
H

S

D

S

A

A
|
A

P

V

T

S

S

E

P

E

P

N

M

A

M

L

V

F

Q

R

L

H

F

V

L

E

E

M

N

Q

L

I

R

A

A

R

Y

F

Q

E

A

R

G

G

E

E

A

P

L

L

R

Q

G

F

E

V

V

I

D

D

F

R

S

A

R

A

G

G

Y
|
Y

active site: E261 (= E254), H280 (= H273)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A72 (= A70), R90 (= R88), M100 (= M97), G144 (= G141), L145 (≠ T142), G146 (= G143), I147 (≠ D144), L148 (≠ I145), W166 (≠ I163), S167 (≠ A164), R168 (≠ S165), T169 (≠ E166), K171 (≠ R168), L198 (= L196), P199 (= P197), A230 (≠ V223), G231 (= G224), R232 (= R225), H280 (= H273), A283 (= A276), Y317 (= Y310)

3kboA 2.14 angstrom crystal structure of putative oxidoreductase (ycdw) from salmonella typhimurium in complex with NADP
37% identity, 56% coverage: 136:310/310 of query aligns to 138:312/312 of 3kboA

query
sites
3kboA

K

S

V

V

L

G

I

I

V

M

G
|
G

T
x
A

G
|
G

D
x
V

I
x
L

G

G

Q

A

R

K

V

V

A

A

Q

E

F

S

L

L

M

Q

P

A

F

W

G

G

V

F

Q

P

L

L

Y

R

G

-

I

C

A
x
W

S
|
S

E
x
R

A
x
S

R

R

T
x
K

L

S

A

W

P

P

F

G

I

V

E

E

-

S

-

Y

V

V

G

G

A

R

L

-

K

E

D

E

L

L

P

R

R

A

L

F

V

L

G

N

D

Q

M

T

D

R

Y

V

V

L

I

I

N

N

L
|
L

L

L

P
|
P

N

N

T

T

P

A

D

Q

T

T

H

V

D

G

I

I

Y

I

D

N

A

S

A

E

L

L

F

L

K

D

Q

Q

F

L

K

P

P

D

S

G

G

A

L

Y

F

V

I

L

N

N

V
x
L

G
x
A

R
|
R

G

G

V

V

A

H

V

V

V

Q

D

E

A

A

D

D

L

L

V

L

Q

A

A

A

L

L

K

D

D

S

G

G

H

K

L

L

A

K

G

G

A

A

V

M

I

L

D
|
D

V

V

C

F

R

S

Q

Q

E
|
E

P

P

L

L

P

P

Q

Q

R

E

H

S

P

P

F

L

W

W

T

R

A

H

W

P

G

R

L

V

L

A

L

M

T

T

G

P

H
|
H

S

I

S

A

A
|
A

P

V

T

T

S

R

P

P

P

A

M

E

M

A

V

I

Q

D

L

Y

F

I

L

S

E

R

N

T

L

I

R

T

A

Q

Y

L

Q

E

A

K

G

G

E

E

A

P

L

V

R

T

G

G

E

Q

V

V

D

D

F

R

S

A

R

R

G

G

Y
|
Y

active site: R227 (= R225), E256 (= E254), H275 (= H273)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G143 (= G141), A144 (≠ T142), G145 (= G143), V146 (≠ D144), L147 (≠ I145), W165 (≠ A164), S166 (= S165), R167 (≠ E166), S168 (≠ A167), K170 (≠ T169), L197 (= L195), P199 (= P197), L225 (≠ V223), A226 (≠ G224), R227 (= R225), D251 (= D249), H275 (= H273), A278 (= A276), Y312 (= Y310)

Sites not aligning to the query:

4z0pA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc02828 (smghra) from sinorhizobium meliloti in complex with NADPH and oxalate (see paper)
29% identity, 95% coverage: 17:310/310 of query aligns to 19:316/316 of 4z0pA

query
sites
4z0pA

L

L

R

A

Q

G

A

A

A

F

P

P

D

G

I

R

E

E

V

V

L

I

T

D

S

L

G

A

D

D

S

P

A

A

E

H

L

Q

S

E

R

R

L

D

A

L

T

S

D

G

C

I

P

D

-

Y

-

A

-

V

V

V

W
|
W

L

K

G

S

Q

A

P

P

D

D

L

L

L

F

A

S

T

-

L

-

R

-

Q

R

G

A

H

P

Q

D

P

L

R

K

W

V

L

V

Q

F

S

S

T

G

W
x
G

A
|
A

G
|
G

I

V

T

D

P

H

L

V

L

L

A

T

D

L

G

P

L

G

S

L

R

P

D

D

Y

V

R

P

L

L

T

V

R
|
R

A

F

V

V

G

D

-

R

I

T

F
x
L

G

T

Q

T

V

R

M
|
M

A

S

E

E

Y

W

V

V

L

M

T

M

Y

Q

M

C

L

L

G

L

H

H

E

L

R

R

E

Q

V
x
H

L
x
R

A

A

R

Y

L

E

V

A

S

L

Q

A

V

K

E

K

R

H

K

E

W

W

D

R

N

D

R

L

Q

S

G

Q

Q

P

G

E

L

A

A

A

G

D

R

V

K

T

V

V

L

G

I

I

V

M

G

G

T
x
M

G
|
G

D
x
V

I
x
L

G

G

Q

Q

R

D

V

A

A

A

Q

R

F

K

L

L

M

A

P

A

F

M

G

G

V

F

Q

K

L

V

Y

I

G

G

I
x
W

A
x
S

S
x
R

E
x
S

A

K

R
|
R

T

V

L

I

A

E

P

G

F

-

I

V

E

E

V

T

G

Y

A

D

L

A

K

A

D

G

L

L

P

D

R

A

L

F

V

L

G

G

D

R

M

T

D

D

Y

F

V

L

I

V

N

G

L

L

L
|
L

P
|
P

N

L

T

T

P

P

D

D

T

T

H

R

D

G

I

I

Y

F

D

N

A

A

L

L

F

F

K

A

Q

K

F

L

K

S

P

R

S

N

G

G

-

P

-

F

-

G

-

A

-

P

L

V

F

F

I

I

N

N

V
x
A

G
|
G

R
|
R

G

G

V

G

A

S

V

Q

V

V

D

E

A

A

D

D

L

I

V

L

Q

E

A

C

L

L

K

D

D

S

G

G

H

V

L

L

A
x
G

G

G

A

A

V

S

I

L

D
|
D

V

V

C

F

R

E

Q

R

E
|
E

P

P

L

L

P

S

Q

P

R

E

H

S

P

R

F

F

W

W

T

D

A

M

W

P

G

N

L

V

L

Y

L

V

T

T

G

P

H
|
H

S

V

S
x
A

A
|
A

P

S

T

S

S

D

P

V

P

R

M

A

M

L

V

F

Q

V

L

H

F

V

L

E

E

H

N

Q

L

I

R

A

A

R

Y

F

Q

E

A

S

G

G

E

L

A

P

L

L

R

E

G

H

E

V

V

V

D

D

F

K

S

V

R

A

G

G

Y
|
Y

active site: L95 (≠ F93), R231 (= R225), G250 (≠ A244), D255 (= D249), E260 (= E254), H279 (= H273)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R89 (= R88), M99 (= M97), M144 (≠ T142), G145 (= G143), V146 (≠ D144), L147 (≠ I145), W165 (≠ I163), S166 (≠ A164), R167 (≠ S165), S168 (≠ E166), R170 (= R168), L197 (= L196), P198 (= P197), A229 (≠ V223), G230 (= G224), R231 (= R225), H279 (= H273), A281 (≠ S275), A282 (= A276), Y316 (= Y310)
binding oxalic acid: W50 (= W45), G70 (≠ W69), A71 (= A70), G72 (= G71), H114 (≠ V112), R115 (≠ L113), R231 (= R225), H279 (= H273)

4weqA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc02828 (smghra) from sinorhizobium meliloti in complex with NADP and sulfate (see paper)
29% identity, 95% coverage: 17:310/310 of query aligns to 19:316/316 of 4weqA

query
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4weqA

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active site: L95 (≠ F93), R231 (= R225), G250 (≠ A244), D255 (= D249), E260 (= E254), H279 (= H273)
binding nadp nicotinamide-adenine-dinucleotide phosphate: R89 (= R88), M99 (= M97), M144 (≠ T142), G145 (= G143), V146 (≠ D144), L147 (≠ I145), W165 (≠ I163), S166 (≠ A164), R167 (≠ S165), S168 (≠ E166), R170 (= R168), L197 (= L196), P198 (= P197), A229 (≠ V223), G230 (= G224), R231 (= R225), D255 (= D249), H279 (= H273), A281 (≠ S275), Y316 (= Y310)

4zqbB Crystal structure of NADP-dependent dehydrogenase from rhodobactersphaeroides in complex with NADP and sulfate
26% identity, 94% coverage: 19:310/310 of query aligns to 45:316/316 of 4zqbB

query
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4zqbB

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active site: L99 (≠ F93), R231 (= R225), E260 (= E254), H279 (= H273)
binding nadp nicotinamide-adenine-dinucleotide phosphate: R93 (= R88), M103 (= M97), G147 (= G141), L148 (≠ T142), G149 (= G143), E150 (≠ D144), L151 (≠ I145), W169 (≠ I163), S170 (≠ A164), R171 (≠ S165), S172 (≠ E166), K174 (≠ R168), L202 (= L196), P203 (= P197), F229 (≠ V223), R231 (= R225), H279 (= H273), S281 (= S275), A282 (= A276), Y316 (= Y310)

Q9UBQ7 Glyoxylate reductase/hydroxypyruvate reductase; EC 1.1.1.79; EC 1.1.1.81 from Homo sapiens (Human) (see paper)
33% identity, 57% coverage: 129:306/310 of query aligns to 148:328/328 of Q9UBQ7

query
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Q9UBQ7

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