SitesBLAST
Comparing AO356_04910 FitnessBrowser__pseudo5_N2C3_1:AO356_04910 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
A0A0K2JL82 Nitrosuccinate lyase; EC 4.3.99.5 from Streptomyces cremeus (see paper)
42% identity, 94% coverage: 22:449/454 of query aligns to 35:471/476 of A0A0K2JL82
- N93 (= N80) mutation to A: Slight decrease in activity.
- D125 (= D112) mutation D->N,V: Almost loss of activity.
- R137 (≠ Q124) binding fumarate
- R140 (≠ G127) binding fumarate
- R201 (≠ K188) binding fumarate
- H253 (= H231) mutation to A: Loss of activity.
- S302 (= S280) mutation to A: Loss of activity.
- K308 (= K286) binding fumarate; mutation to A: Loss of activity.
- N310 (= N288) binding fumarate; mutation to A: Loss of activity.
- R341 (= R319) mutation to A: Loss of activity.
5xnzA Crystal structure of cred complex with fumarate (see paper)
41% identity, 94% coverage: 22:447/454 of query aligns to 21:438/439 of 5xnzA
2x75A Staphylococcus aureus adenylosuccinate lyase (see paper)
30% identity, 95% coverage: 14:445/454 of query aligns to 4:424/427 of 2x75A
Sites not aligning to the query:
P12047 Adenylosuccinate lyase; ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor; EC 4.3.2.2 from Bacillus subtilis (strain 168) (see paper)
30% identity, 94% coverage: 14:439/454 of query aligns to 5:422/431 of P12047
- H89 (= H105) mutation to Q: Abolishes enzyme activity.
- H141 (≠ W157) mutation to Q: Abolishes enzyme activity.
- Q212 (= Q233) mutation to E: Decreases catalytic activity 1000-fold.; mutation to M: Abolishes enzyme activity.
- N270 (= N288) mutation N->D,L: Abolishes enzyme activity.
- R301 (= R319) mutation R->K,Q: Abolishes enzyme activity.
Q9X0I0 Adenylosuccinate lyase; ASL; Adenylosuccinase; ASase; EC 4.3.2.2 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
26% identity, 94% coverage: 14:439/454 of query aligns to 5:422/431 of Q9X0I0
- H141 (≠ W157) active site, Proton donor/acceptor
4eeiB Crystal structure of adenylosuccinate lyase from francisella tularensis complexed with amp and succinate
24% identity, 92% coverage: 14:430/454 of query aligns to 5:412/423 of 4eeiB
- active site: H67 (≠ N80), S140 (≠ T156), H141 (≠ W157), K256 (= K286), E263 (≠ A293)
- binding adenosine monophosphate: K66 (≠ G79), H67 (≠ N80), D68 (≠ S81), Q212 (= Q233), R289 (= R319), I291 (≠ L321), S294 (≠ W324), R298 (≠ W328)
5hw2A Crystal structure of adenylosuccinate lyase from francisella tularensis complexed with fumaric acid
24% identity, 92% coverage: 14:430/454 of query aligns to 5:408/419 of 5hw2A
P30566 Adenylosuccinate lyase; ADSL; ASL; Adenylosuccinase; ASase; EC 4.3.2.2 from Homo sapiens (Human) (see 13 papers)
26% identity, 96% coverage: 14:450/454 of query aligns to 21:465/484 of P30566
- M26 (= M19) to L: in ADSLD; severe; dbSNP:rs1311171245
- I72 (≠ Y66) to V: in ADSLD; severe
- P100 (≠ A94) to A: in ADSLD; moderate; dbSNP:rs119450942
- Y114 (≠ D112) to H: in ADSLD; severe; total loss of activity; dbSNP:rs374259530
- R141 (≠ I139) to W: in ADSLD; severe; dbSNP:rs756210458
- H159 (≠ W157) active site, Proton donor/acceptor
- R190 (≠ K188) to Q: in ADSLD; moderate; dbSNP:rs28941471
- R194 (≠ L192) to C: in ADSLD; severe; reduces protein stability; dbSNP:rs1465152683
- K246 (≠ Q233) to E: in ADSLD; moderate; strongly reduced catalytic activity; dbSNP:rs119450944
- D268 (= D257) to N: in ADSLD; severe; total loss of activity; dbSNP:rs746501563
- S289 (= S280) active site, Proton donor/acceptor
- R303 (≠ V294) to C: in ADSLD; mild; strongly reduced activity with SAMP, but only slightly reduced activity with SAICAR; abolishes cooperativity; dbSNP:rs373458753
- L311 (≠ V302) to V: in ADSLD; severe; slightly reduced enzyme activity; dbSNP:rs2044791112
- P318 (≠ T308) to L: in ADSLD; severe; dbSNP:rs202064195
- V364 (= V354) to M: in ADSLD; severe; dbSNP:rs370851726
- R374 (≠ E364) to W: in ADSLD; severe; dbSNP:rs376533026
- S395 (vs. gap) to R: in ADSLD; severe
- R396 (= R385) to C: in ADSLD; severe; abolishes cooperativity and reduces enzyme activity; dbSNP:rs755492501; to H: in ADSLD; severe; abolishes cooperativity and reduces enzyme activity; dbSNP:rs763542069
- D422 (≠ V409) to Y: in ADSLD; moderate; dbSNP:rs119450943
- L423 (= L410) to V: in ADSLD; moderate
- R426 (≠ E413) to H: in ADSLD; severe; most frequent mutation; dbSNP:rs119450941
- D430 (≠ T417) to N: in ADSLD; mild; dbSNP:rs554254383
- S438 (≠ T423) to P: in ADSLD; severe; dbSNP:rs119450940
- S447 (≠ A432) to P: in ADSLD; severe; dbSNP:rs777821034
- T450 (≠ L435) to S: in ADSLD; moderate; dbSNP:rs372895468
- R452 (≠ Q437) to P: in ADSLD; severe; dbSNP:rs775671027
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine; A → V: in ADSLD; severe; dbSNP:rs143083947
- 3 A → V: in ADSLD; severe
5nx9D Crystal structure of neanderthal adenylosuccinate lyase (adsl) in complex with its products amp and fumarate (see paper)
26% identity, 96% coverage: 14:450/454 of query aligns to 14:458/477 of 5nx9D
- active site: H79 (≠ N80), T151 (= T156), H152 (≠ W157), S283 (= S281), K288 (= K286), E295 (≠ A293)
- binding 2-[9-(3,4-dihydroxy-5-phosphonooxymethyl-tetrahydro-furan-2-yl)-9h-purin-6-ylamino]-succinic acid: T151 (= T156), H152 (≠ W157)
- binding adenosine monophosphate: Y14 (≠ F14), R78 (≠ G79), H79 (≠ N80), D80 (≠ S81), S105 (= S110), Q234 (= Q228), R296 (≠ V294), L324 (= L321), S327 (≠ W324), A328 (≠ H325), R331 (≠ W328)
- binding fumaric acid: H79 (≠ N80), S105 (= S110), Q234 (= Q228), S282 (= S280), S283 (= S281), K288 (= K286)
Sites not aligning to the query:
5nxaB Crystal structure of neanderthal adenylosuccinate lyase (adsl)in complex with its products aicar and fumarate (see paper)
27% identity, 77% coverage: 103:450/454 of query aligns to 36:396/415 of 5nxaB
- active site: T89 (= T156), H90 (≠ W157), S221 (= S281), K226 (= K286), E233 (≠ A293)
- binding aminoimidazole 4-carboxamide ribonucleotide: M230 (≠ V290), R234 (≠ V294)
- binding fumaric acid: S220 (= S280), S221 (= S281), M223 (= M283), K226 (= K286), N228 (= N288)
- binding n-{[5-amino-1-(5-o-phosphono-beta-d-arabinofuranosyl)-1h-imidazol-4-yl]carbonyl}-l-aspartic acid: S43 (= S110), T89 (= T156), H90 (≠ W157), Q172 (= Q228), L262 (= L321), S265 (≠ W324), A266 (≠ H325), R269 (≠ W328)
Sites not aligning to the query:
P24058 Argininosuccinate lyase; ASAL; Arginosuccinase; Delta crystallin II; Delta-2 crystallin; EC 4.3.2.1 from Anas platyrhynchos (Mallard) (Anas boschas) (see 4 papers)
28% identity, 57% coverage: 104:363/454 of query aligns to 109:366/468 of P24058