SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO356_04925 FitnessBrowser__pseudo5_N2C3_1:AO356_04925 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P00437 Protocatechuate 3,4-dioxygenase beta chain; 3,4-PCD; EC 1.13.11.3 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
51% identity, 94% coverage: 16:234/234 of query aligns to 18:238/239 of P00437

query
sites
P00437

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Y109 (= Y105) binding
Y148 (= Y144) binding
H161 (= H157) binding
H163 (= H159) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4whrB Anhydride reaction intermediate trapped in protocatechuate 3,4- dioxygenase (pseudomonas putida) at ph 8.5 (see paper)
51% identity, 94% coverage: 16:234/234 of query aligns to 17:237/238 of 4whrB

query
sites
4whrB

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active site: Y108 (= Y105), Y147 (= Y144), R157 (= R154), H160 (= H157), H162 (= H159)
binding 4-fluorobenzene-1,2-diol: P22 (= P21), I28 (≠ N27), S38 (≠ F37), P40 (= P39), P40 (= P39), Q41 (≠ H40), R231 (≠ D228), E236 (= E233)
binding 4-fluorooxepine-2,7-dione: Y147 (= Y144), W149 (= W146), R157 (= R154), H160 (= H157), H162 (= H159), I191 (= I188)
binding fe (iii) ion: Y108 (= Y105), Y147 (= Y144), H160 (= H157), H162 (= H159)

Sites not aligning to the query:

binding 4-fluorobenzene-1,2-diol: 7

4whqB Alkylperoxo reaction intermediate trapped in protocatechuate 3,4- dioxygenase (pseudomonas putida) at ph 6.5 (see paper)
51% identity, 94% coverage: 16:234/234 of query aligns to 17:237/238 of 4whqB

query
sites
4whqB

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active site: Y108 (= Y105), Y147 (= Y144), R157 (= R154), H160 (= H157), H162 (= H159)
binding 4-fluorobenzene-1,2-diol: R83 (= R80), R109 (≠ N106), H110 (= H107), K111 (≠ D108), K193 (≠ N190), I195 (≠ V192), A196 (≠ P193), V201 (≠ K198), D224 (= D221)
binding (6S)-4-fluoro-6-hydroperoxy-6-hydroxycyclohexa-2,4-dien-1-one: Y108 (= Y105), Y147 (= Y144), W149 (= W146), R157 (= R154), H160 (= H157), H162 (= H159), I191 (= I188)
binding fe (iii) ion: Y108 (= Y105), Y147 (= Y144), H160 (= H157), H162 (= H159)

4whqF Alkylperoxo reaction intermediate trapped in protocatechuate 3,4- dioxygenase (pseudomonas putida) at ph 6.5 (see paper)
51% identity, 93% coverage: 16:233/234 of query aligns to 17:236/236 of 4whqF

query
sites
4whqF

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active site: Y108 (= Y105), Y147 (= Y144), R157 (= R154), H160 (= H157), H162 (= H159)
binding 4-fluorobenzene-1,2-diol: S38 (≠ F37), I39 (≠ L38), P40 (= P39), N66 (≠ T63), G124 (≠ T121), V126 (≠ T123), K193 (≠ N190), I195 (≠ V192), A196 (≠ P193), V201 (≠ K198)
binding bicarbonate ion: R109 (≠ N106), H110 (= H107)
binding fe (iii) ion: Y108 (= Y105), H160 (= H157), H162 (= H159)

3pckM Structure of protocatechuate 3,4-dioxygenase complexed with 6- hydroxynicotinic acid n-oxide (see paper)
52% identity, 93% coverage: 16:233/234 of query aligns to 17:233/233 of 3pckM

query
sites
3pckM

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active site: Y105 (= Y105), Y144 (= Y144), R154 (= R154), H157 (= H157), H159 (= H159)
binding fe (iii) ion: Y105 (= Y105), H157 (= H157), H159 (= H159)
binding 6-hydroxyisonicotinic acid n-oxide: Y24 (= Y23), Y105 (= Y105), W146 (= W146), R154 (= R154), H157 (= H157), H159 (= H159), I188 (= I188)

3pcjM Structure of protocatechuate 3,4-dioxygenase complexed with 2- hydroxyisonicotinic acid n-oxide (see paper)
52% identity, 93% coverage: 16:233/234 of query aligns to 17:233/233 of 3pcjM

query
sites
3pcjM

P

P

E

K

Y

A

L

L

H

T

P

P

P

D

Y
|
Y

Q

K

S

T

T

S

N

I

L

A

R

R

S

S

P

P

S

R

K

Q

P

A

L

L

V

V

F

S

L

I

P

P

H

Q

S

S

L

I

S

S

E

E

I

T

T

T

G

G

P

P

T

N

V

F

G

S

A

H

D

L

R

G

V

F

Q

G

E

A

K

H

D

D

Y

H

D

D

L

L

T

L

A

L

Q

N

H

F

A

-

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

I

V

H

A

G

G

R

R

V

V

L

V

D

D

E

Q

H

Y

G

G

Q

K

P

P

V

V

P

P

G

N

I

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

N

R

H

H

D

K

R

N

D

D

N

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

T

G

G

G

T

V

G

G

R

R

T

C

V

L

T

T

D

D

A

S

D

D

G

G

W

Y

Y

Y

Q

S

F

F

Q

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W
|
W

G

R

N

N

H

G

H

P

N

N

A

D

W

W

R
|
R

P

P

A

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

P

P

S

S

I

I

L

A

T

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

E

P

Y

M

D

C

P

P

I

I

Y

V

N

K

C

S

V

I

P

A

D

N

T

P

S

E

A

A

K

V

E

Q

R

Q

L

L

I

I

S

A

S

K

F

L

D

D

L

M

E

N

K

N

T

A

I

N

P

P

N

M

Y

D

A

C

L

L

G

A

Y

Y

R

R

W

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

D

R

A

K

T

T

P

H

M

F

E

E

active site: Y105 (= Y105), Y144 (= Y144), R154 (= R154), H157 (= H157), H159 (= H159)
binding fe (iii) ion: Y105 (= Y105), H157 (= H157), H159 (= H159)
binding 2-hydroxyisonicotinic acid n-oxide: Y24 (= Y23), Y144 (= Y144), W146 (= W146), R154 (= R154), H157 (= H157), H159 (= H159)

3pciM Structure of protocatechuate 3,4-dioxygenase complexed with 3-iodo-4- hydroxybenzoate (see paper)
52% identity, 93% coverage: 16:233/234 of query aligns to 17:233/233 of 3pciM

query
sites
3pciM

P

P

E

K

Y

A

L

L

H

T

P

P

P

D

Y

Y

Q

K

S

T

T

S

N

I

L

A

R

R

S

S

P

P

S

R

K

Q

P

A

L

L

V

V

F

S

L

I

P

P

H

Q

S

S

L

I

S

S

E

E

I

T

T

T

G

G

P

P

T

N

V

F

G

S

A

H

D

L

R

G

V

F

Q

G

E

A

K

H

D

D

Y

H

D

D

L

L

T

L

A

L

Q

N

H

F

A

-

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

I

V

H

A

G

G

R

R

V

V

L

V

D

D

E

Q

H

Y

G

G

Q

K

P

P

V

V

P

P

G

N

I

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

N

R

H

H

D

K

R

N

D

D

N

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

T

G

G

G

T

V

G

G

R

R

T

C

V

L

T

T

D

D

A

S

D

D

G

G

W

Y

Y

Y

Q

S

F

F

Q

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W

W

G

R

N

N

H

G

H

P

N

N

A

D

W

W

R
|
R

P

P

A

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

P

P

S

S

I

I

L

A

T

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

E

P

Y

M

D

C

P

P

I

I

Y

V

N

K

C

S

V

I

P

A

D

N

T

P

S

E

A

A

K

V

E

Q

R

Q

L

L

I

I

S

A

S

K

F

L

D

D

L

M

E

N

K

N

T

A

I

N

P

P

N

M

Y

D

A

C

L

L

G

A

Y

Y

R

R

W

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

D

R

A

K

T

T

P

H

M

F

E

E

active site: Y105 (= Y105), Y144 (= Y144), R154 (= R154), H157 (= H157), H159 (= H159)
binding fe (iii) ion: Y105 (= Y105), Y144 (= Y144), H157 (= H157), H159 (= H159)
binding 3-iodo-4-hydroxybenzoic acid: Y105 (= Y105), Y144 (= Y144), H159 (= H159)

3pchM Structure of protocatechuate 3,4-dioxygenase complexed with 3-chloro- 4-hydroxybenzoate (see paper)
52% identity, 93% coverage: 16:233/234 of query aligns to 17:233/233 of 3pchM

query
sites
3pchM

P

P

E

K

Y

A

L

L

H

T

P

P

P

D

Y
|
Y

Q

K

S

T

T

S

N

I

L

A

R

R

S

S

P

P

S

R

K

Q

P

A

L

L

V

V

F

S

L

I

P

P

H

Q

S

S

L

I

S

S

E

E

I

T

T

T

G

G

P

P

T

N

V

F

G

S

A

H

D

L

R

G

V

F

Q

G

E

A

K

H

D

D

Y

H

D

D

L

L

T

L

A

L

Q

N

H

F

A

-

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

I

V

H

A

G

G

R

R

V

V

L

V

D

D

E

Q

H

Y

G

G

Q

K

P

P

V

V

P

P

G

N

I

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

N

R

H

H

D

K

R

N

D

D

N

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

T

G

G

G

T

V

G

G

R

R

T

C

V

L

T

T

D

D

A

S

D

D

G

G

W

Y

Y

Y

Q

S

F

F

Q

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W
|
W

G

R

N

N

H

G

H

P

N

N

A

D

W

W

R
|
R

P

P

A

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

P

P

S

S

I

I

L

A

T

T

R

K

L

L

V

I

T

T

Q
|
Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

E

P

Y

M

D

C

P

P

I
|
I

Y

V

N

K

C

S

V

I

P

A

D

N

T

P

S

E

A

A

K

V

E

Q

R

Q

L

L

I

I

S

A

S

K

F

L

D

D

L

M

E

N

K

N

T

A

I

N

P

P

N

M

Y

D

A

C

L

L

G

A

Y

Y

R

R

W

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

D

R

A

K

T

T

P

H

M

F

E

E

active site: Y105 (= Y105), Y144 (= Y144), R154 (= R154), H157 (= H157), H159 (= H159)
binding 3-chloro-4-hydroxybenzoic acid: Y24 (= Y23), Y144 (= Y144), W146 (= W146), R154 (= R154), H157 (= H157), H159 (= H159), Q174 (= Q174), I188 (= I188)
binding fe (iii) ion: Y105 (= Y105), Y144 (= Y144), H157 (= H157), H159 (= H159)

3pcgM Structure of protocatechuate 3,4-dioxygenase complexed with the inhibitor 4-hydroxyphenylacetate (see paper)
52% identity, 93% coverage: 16:233/234 of query aligns to 17:233/233 of 3pcgM

query
sites
3pcgM

P

P

E

K

Y

A

L

L

H

T

P

P

P

D

Y
|
Y

Q

K

S

T

T

S

N

I

L

A

R

R

S

S

P

P

S

R

K

Q

P

A

L

L

V

V

F

S

L

I

P

P

H

Q

S

S

L

I

S

S

E

E

I

T

T

T

G

G

P

P

T

N

V

F

G

S

A

H

D

L

R

G

V

F

Q

G

E

A

K

H

D

D

Y

H

D

D

L

L

T

L

A

L

Q

N

H

F

A

-

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

I

V

H

A

G

G

R

R

V

V

L

V

D

D

E

Q

H

Y

G

G

Q

K

P

P

V

V

P

P

G

N

I

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

N

R

H

H

D

K

R

N

D

D

N

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

T

G

G

G

T

V

G

G

R

R

T

C

V

L

T

T

D

D

A

S

D

D

G

G

W

Y

Y

Y

Q

S

F

F

Q

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W
|
W

G

R

N

N

H

G

H

P

N

N

A

D

W

W

R
|
R

P

P

A

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

P

P

S

S

I

I

L

A

T

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

E

P

Y

M

D

C

P

P

I
|
I

Y

V

N

K

C

S

V

I

P

A

D

N

T

P

S

E

A

A

K

V

E

Q

R

Q

L

L

I

I

S

A

S

K

F

L

D

D

L

M

E

N

K

N

T

A

I

N

P

P

N

M

Y

D

A

C

L

L

G

A

Y

Y

R

R

W

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

D

R

A

K

T

T

P

H

M

F

E

E

active site: Y105 (= Y105), Y144 (= Y144), R154 (= R154), H157 (= H157), H159 (= H159)
binding 4-hydroxyphenylacetate: Y24 (= Y23), Y105 (= Y105), Y144 (= Y144), W146 (= W146), R154 (= R154), H159 (= H159), I188 (= I188)
binding fe (iii) ion: Y105 (= Y105), Y144 (= Y144), H157 (= H157), H159 (= H159)

3pcfM Structure of protocatechuate 3,4-dioxygenase complexed with 3-fluro-4- hydroxybenzoate (see paper)
52% identity, 93% coverage: 16:233/234 of query aligns to 17:233/233 of 3pcfM

query
sites
3pcfM

P

P

E

K

Y

A

L

L

H

T

P
|
P

P

D

Y
|
Y

Q

K

S

T

T

S

N
x
I

L

A

R

R

S

S

P

P

S

R

K

Q

P

A

L

L

V

V

F

S

L

I

P

P

H

Q

S

S

L

I

S

S

E

E

I

T

T

T

G

G

P

P

T

N

V

F

G

S

A

H

D

L

R

G

V

F

Q

G

E

A

K

H

D

D

Y

H

D

D

L

L

T

L

A

L

Q

N

H

F

A

-

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

I

V

H

A

G

G

R

R

V

V

L

V

D

D

E

Q

H

Y

G

G

Q

K

P

P

V

V

P

P

G

N

I

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

N

R

H

H

D

K

R

N

D

D

N

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

T

G

G

G

T

V

G

G

R

R

T

C

V

L

T

T

D

D

A

S

D

D

G

G

W

Y

Y

Y

Q

S

F

F

Q

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W
|
W

G

R

N

N

H

G

H

P

N

N

A

D

W

W

R
|
R

P

P

A

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

P

P

S

S

I

I

L

A

T

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

E

P

Y

M

D

C

P

P

I
|
I

Y

V

N

K

C

S

V

I

P

A

D

N

T

P

S

E

A

A

K

V

E

Q

R

Q

L

L

I

I

S

A

S

K

F

L

D

D

L

M

E

N

K

N

T

A

I

N

P

P

N

M

Y

D

A

C

L

L

G

A

Y

Y

R

R

W

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

D

R

A

K

T

T

P

H

M

F

E

E

active site: Y105 (= Y105), Y144 (= Y144), R154 (= R154), H157 (= H157), H159 (= H159)
binding fe (iii) ion: Y105 (= Y105), Y144 (= Y144), H157 (= H157), H159 (= H159)
binding 3-fluoro-4-hydroxybenzoic acid: P22 (= P21), Y24 (= Y23), I28 (≠ N27), Y144 (= Y144), W146 (= W146), R154 (= R154), H157 (= H157), H159 (= H159), I188 (= I188)

3pceM Structure of protocatechuate 3,4-dioxygenase complexed with 3- hydroxyphenylacetate (see paper)
52% identity, 93% coverage: 16:233/234 of query aligns to 17:233/233 of 3pceM

query
sites
3pceM

P

P

E

K

Y

A

L

L

H

T

P

P

P

D

Y

Y

Q

K

S

T

T

S

N

I

L

A

R

R

S

S

P

P

S

R

K

Q

P

A

L

L

V

V

F

S

L

I

P

P

H

Q

S

S

L

I

S

S

E

E

I

T

T

T

G

G

P

P

T

N

V

F

G

S

A

H

D

L

R

G

V

F

Q

G

E

A

K

H

D

D

Y

H

D

D

L

L

T

L

A

L

Q

N

H

F

A

-

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

I

V

H

A

G

G

R

R

V

V

L

V

D

D

E

Q

H

Y

G

G

Q

K

P

P

V

V

P

P

G

N

I

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

N

R

H

H

D

K

R

N

D

D

N

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

T

G

G

G

T

V

G

G

R

R

T

C

V

L

T

T

D

D

A

S

D

D

G

G

W

Y

Y

Y

Q

S

F

F

Q

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W
|
W

G

R

N

N

H

G

H

P

N

N

A

D

W

W

R
|
R

P

P

A

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

P

P

S

S

I

I

L

A

T

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

E

P

Y

M

D

C

P

P

I
|
I

Y

V

N

K

C

S

V

I

P

A

D

N

T

P

S

E

A

A

K

V

E

Q

R

Q

L

L

I

I

S

A

S

K

F

L

D

D

L

M

E

N

K

N

T

A

I

N

P

P

N

M

Y

D

A

C

L

L

G

A

Y

Y

R

R

W

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

D

R

A

K

T

T

P

H

M

F

E

E

active site: Y105 (= Y105), Y144 (= Y144), R154 (= R154), H157 (= H157), H159 (= H159)
binding 3-hydroxyphenylacetate: Y105 (= Y105), Y144 (= Y144), W146 (= W146), R154 (= R154), H159 (= H159), I188 (= I188)
binding fe (iii) ion: Y105 (= Y105), Y144 (= Y144), H157 (= H157), H159 (= H159)

3pcbM Structure of protocatechuate 3,4-dioxygenase complexed with 3- hydroxybenzoate (see paper)
52% identity, 93% coverage: 16:233/234 of query aligns to 17:233/233 of 3pcbM

query
sites
3pcbM

P

P

E

K

Y

A

L

L

H

T

P

P

P

D

Y
|
Y

Q

K

S

T

T

S

N

I

L

A

R

R

S

S

P

P

S

R

K

Q

P

A

L

L

V

V

F

S

L

I

P

P

H

Q

S

S

L

I

S

S

E

E

I

T

T

T

G

G

P

P

T

N

V

F

G

S

A

H

D

L

R

G

V

F

Q

G

E

A

K

H

D

D

Y

H

D

D

L

L

T

L

A

L

Q

N

H

F

A

-

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

I

V

H

A

G

G

R

R

V

V

L

V

D

D

E

Q

H

Y

G

G

Q

K

P

P

V

V

P

P

G

N

I

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

N

R

H

H

D

K

R

N

D

D

N

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

T

G

G

G

T

V

G

G

R

R

T

C

V

L

T

T

D

D

A

S

D

D

G

G

W

Y

Y

Y

Q

S

F

F

Q

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W
|
W

G

R

N

N

H

G

H

P

N

N

A

D

W

W

R
|
R

P

P

A

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

P

P

S

S

I

I

L

A

T

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

E

P

Y

M

D

C

P

P

I
|
I

Y

V

N

K

C

S

V

I

P

A

D

N

T

P

S

E

A

A

K

V

E

Q

R

Q

L

L

I

I

S

A

S

K

F

L

D

D

L

M

E

N

K

N

T

A

I

N

P

P

N

M

Y

D

A

C

L

L

G

A

Y

Y

R

R

W

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

D

R

A

K

T

T

P

H

M

F

E

E

active site: Y105 (= Y105), Y144 (= Y144), R154 (= R154), H157 (= H157), H159 (= H159)
binding 3-hydroxybenzoic acid: Y24 (= Y23), Y144 (= Y144), W146 (= W146), R154 (= R154), I188 (= I188)
binding fe (iii) ion: Y105 (= Y105), Y144 (= Y144), H157 (= H157), H159 (= H159)

3lxvM Tyrosine 447 of protocatechuate 3,4-dioxygenase controls efficient progress through catalysis
50% identity, 94% coverage: 15:234/234 of query aligns to 16:237/238 of 3lxvM

query
sites
3lxvM

Q

H

P

P

E

K

Y

A

L

L

H

T

P

P

P

D

Y
|
Y

Q

K

S

T

T

S

N

I

L

A

R

R

S

S

P

P

S

R

K

Q

P

A

L

L

V

V

F

S

L

I

P

P

H

Q

S

S

L

I

S

S

E

E

I

T

T

T

G

G

P

P

T

N

V

F

G

S

A

H

D

L

R

G

V

F

Q

G

E

A

K

H

D

D

Y

H

D

D

L

L

-

L

-

L

T

N

A

F

Q

N

H

N

A

G

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

I

V

H

A

G

G

R

R

V

V

L

V

D

D

E

Q

H

Y

G

G

Q

K

P

P

V

V

P

P

G

N

I

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

N

R

H

H

D

K

R

N

D

D

N

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

T

G

G

G

T

V

G

G

R

R

T

C

V

L

T

T

D

D

A

S

D

D

G

G

W

Y

Y

Y

Q

S

F

F

Q

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
x
H

P

P

W
|
W

G

R

N

N

H

G

H

P

N

N

A

D

W

W

R
|
R

P

P

A

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

P

P

S

S

I

I

L

A

T

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

E

P

Y

M

D

C

P

P

I
|
I

Y

V

N

K

C

S

V

I

P

A

D

N

T

P

S

E

A

A

K

V

E

Q

R

Q

L

L

I

I

S

A

S

K

F

L

D

D

L

M

E

N

K

N

T

A

I

N

P

P

N

M

Y

D

A

C

L

L

G

A

Y

Y

R

R

W

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

D

R

A

K

T

T

P

H

M

F

E

E

K

N

binding 4-nitrocatechol: Y24 (= Y23), Y108 (= Y105), H147 (≠ Y144), W149 (= W146), R157 (= R154), H160 (= H157), H162 (= H159), I191 (= I188)
binding fe (iii) ion: Y108 (= Y105), H160 (= H157), H162 (= H159)

3lktM Tyrosine 447 of protocatechuate 3,4-dioxygenase controls efficient progress through catalysis
50% identity, 94% coverage: 15:234/234 of query aligns to 16:237/238 of 3lktM

query
sites
3lktM

Q

H

P

P

E

K

Y

A

L

L

H

T

P

P

P

D

Y

Y

Q

K

S

T

T

S

N

I

L

A

R

R

S

S

P

P

S

R

K

Q

P

A

L

L

V

V

F

S

L

I

P

P

H

Q

S

S

L

I

S

S

E

E

I

T

T

T

G

G

P

P

T

N

V

F

G

S

A

H

D

L

R

G

V

F

Q

G

E

A

K

H

D

D

Y

H

D

D

L

L

-

L

-

L

T

N

A

F

Q

N

H

N

A

G

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

I

V

H

A

G

G

R

R

V

V

L

V

D

D

E

Q

H

Y

G

G

Q

K

P

P

V

V

P

P

G

N

I

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

N

R

H

H

D

K

R

N

D

D

N

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

T

G

G

G

T

V

G

G

R

R

T

C

V

L

T

T

D

D

A

S

D

D

G

G

W

Y

Y

Y

Q

S

F

F

Q

R

T

T

I

I

K

K

P

P

G

G

A

P

Y

H

P

P

W

W

G

R

N

N

H

G

H

P

N

N

A

D

W

W

R

R

P

P

A

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

P

P

S

S

I

I

L

A

T

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

E

P

Y

M

D

C

P

P

I

I

Y

V

N

K

C

S

V

I

P

A

D

N

T

P

S

E

A

A

K

V

E

Q

R

Q

L

L

I

I

S

A

S

K

F

L

D

D

L

M

E

N

K

N

T

A

I

N

P

P

N

M

Y

D

A

C

L

L

G

A

Y

Y

R

R

W

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

D

R

A

K

T

T

P

H

M

F

E

E

K

N

binding fe (iii) ion: Y108 (= Y105), H160 (= H157), H162 (= H159)

3mv4M Axial ligand swapping in double mutant maintains intradiol-cleavage chemistry in protocatechuate 3,4-dioxygenase
50% identity, 94% coverage: 16:234/234 of query aligns to 17:237/238 of 3mv4M

query
sites
3mv4M

P

P

E

K

Y

A

L

L

H

T

P

P

P

D

Y

Y

Q

K

S

T

T

S

N

I

L

A

R

R

S

S

P

P

S

R

K

Q

P

A

L

L

V

V

F

S

L

I

P

P

H

Q

S

S

L

I

S

S

E

E

I

T

T

T

G

G

P

P

T

N

V

F

G

S

A

H

D

L

R

G

V

F

Q

G

E

A

K

H

D

D

Y

H

D

D

L

L

-

L

-

L

T

N

A

F

Q

N

H

N

A

G

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

I

V

H

A

G

G

R

R

V

V

L

V

D

D

E

Q

H

Y

G

G

Q

K

P

P

V

V

P

P

G

N

I

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

N

R

H

H

D

K

R

N

D

D

N

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

T

G

G

G

T

V

G

G

R

R

T

C

V

L

T

T

D

D

A

S

D

D

G

G

W

Y

Y

Y

Q

S

F

F

Q

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
x
H

P

P

W

W

G

R

N

N

H

G

H

P

N

N

A

D

W

W

R
|
R

P

P

A

A

H
|
H

I

I

H
x
Y

F

F

S

G

L

I

F

S

G

G

P

P

S

S

I

I

L

A

T

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

E

P

Y

M

D

C

P

P

I

I

Y

V

N

K

C

S

V

I

P

A

D

N

T

P

S

E

A

A

K

V

E

Q

R

Q

L

L

I

I

S

A

S

K

F

L

D

D

L

M

E

N

K

N

T

A

I

N

P

P

N

M

Y

D

A

C

L

L

G

A

Y

Y

R

R

W

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

D

R

A

K

T

T

P

H

M

F

E

E

K

N

binding carbonate ion: Y108 (= Y105), H147 (≠ Y144), R157 (= R154), Y162 (≠ H159)
binding fe (iii) ion: Y108 (= Y105), H147 (≠ Y144), H160 (= H157), Y162 (≠ H159)

3mi5M Axial ligand swapping in double mutant maintains intradiol-cleavage chemistry in protocatechuate 3,4-dioxygenase
50% identity, 94% coverage: 16:234/234 of query aligns to 17:237/238 of 3mi5M

query
sites
3mi5M

P

P

E

K

Y

A

L

L

H

T

P

P

P

D

Y

Y

Q

K

S

T

T

S

N

I

L

A

R

R

S

S

P

P

S

R

K

Q

P

A

L

L

V

V

F

S

L

I

P

P

H

Q

S

S

L

I

S

S

E

E

I

T

T

T

G

G

P

P

T

N

V

F

G

S

A

H

D

L

R

G

V

F

Q

G

E

A

K

H

D

D

Y

H

D

D

L

L

-

L

-

L

T

N

A

F

Q

N

H

N

A

G

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

I

V

H

A

G

G

R

R

V

V

L

V

D

D

E

Q

H

Y

G

G

Q

K

P

P

V

V

P

P

G

N

I

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

N

R

H

H

D

K

R

N

D

D

N

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

T

G

G

G

T

V

G

G

R

R

T

C

V

L

T

T

D

D

A

S

D

D

G

G

W

Y

Y

Y

Q

S

F

F

Q

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
x
H

P

P

W
|
W

G

R

N

N

H

G

H

P

N

N

A

D

W

W

R
|
R

P

P

A

A

H
|
H

I

I

H
x
Y

F

F

S

G

L

I

F

S

G

G

P

P

S

S

I

I

L

A

T

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

E

P

Y

M

D

C

P

P

I

I

Y

V

N

K

C

S

V

I

P

A

D

N

T

P

S

E

A

A

K

V

E

Q

R

Q

L

L

I

I

S

A

S

K

F

L

D

D

L

M

E

N

K

N

T

A

I

N

P

P

N

M

Y

D

A

C

L

L

G

A

Y

Y

R

R

W

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

D

R

A

K

T

T

P

H

M

F

E

E

K

N

binding catechol: Y108 (= Y105), H147 (≠ Y144), W149 (= W146), R157 (= R154), H160 (= H157), Y162 (≠ H159)
binding fe (iii) ion: Y108 (= Y105), H160 (= H157), Y162 (≠ H159)

3mflM Axial ligand swapping in double mutant maintains intradiol-cleavage chemistry in protocatechuate 3,4-dioxygenase
50% identity, 94% coverage: 16:234/234 of query aligns to 17:237/238 of 3mflM

query
sites
3mflM

P

P

E

K

Y

A

L

L

H

T

P

P

P

D

Y

Y

Q

K

S

T

T

S

N

I

L

A

R

R

S

S

P

P

S

R

K

Q

P

A

L

L

V

V

F

S

L

I

P

P

H

Q

S

S

L

I

S

S

E

E

I

T

T

T

G

G

P

P

T

N

V

F

G

S

A

H

D

L

R

G

V

F

Q

G

E

A

K

H

D

D

Y

H

D

D

L

L

-

L

-

L

T

N

A

F

Q

N

H

N

A

G

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

I

V

H

A

G

G

R

R

V

V

L

V

D

D

E

Q

H

Y

G

G

Q

K

P

P

V

V

P

P

G

N

I

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

N

R

H

H

D

K

R

N

D

D

N

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

T

G

G

G

T

V

G

G

R

R

T

C

V

L

T

T

D

D

A

S

D

D

G

G

W

Y

Y

Y

Q

S

F

F

Q

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
x
H

P

P

W
|
W

G

R

N

N

H

G

H

P

N

N

A

D

W

W

R
|
R

P

P

A

A

H
|
H

I

I

H
x
Y

F

F

S

G

L

I

F

S

G

G

P

P

S

S

I

I

L

A

T

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

E

P

Y

M

D

C

P

P

I

I

Y

V

N

K

C

S

V

I

P

A

D

N

T

P

S

E

A

A

K

V

E

Q

R

Q

L

L

I

I

S

A

S

K

F

L

D

D

L

M

E

N

K

N

T

A

I

N

P

P

N

M

Y

D

A

C

L

L

G

A

Y

Y

R

R

W

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

D

R

A

K

T

T

P

H

M

F

E

E

K

N

binding 2-(3,4-dihydroxyphenyl)acetic acid: Y108 (= Y105), H147 (≠ Y144), W149 (= W146), R157 (= R154), H160 (= H157), Y162 (≠ H159)
binding fe (iii) ion: Y108 (= Y105), H160 (= H157), Y162 (≠ H159)

2buqB Crystal structure of wild-type protocatechuate 3,4-dioxygenase from acinetobacter sp. Adp1 in complex with catechol (see paper)
48% identity, 91% coverage: 21:233/234 of query aligns to 20:234/238 of 2buqB

query
sites
2buqB

P

P

P

G

Y

Y

Q

K

S

T

T

S

N

V

L

L

R

R

S

S

P

P

S

K

K

N

P

A

L

L

V

I

F

S

L

I

P

A

H

E

S

T

L

L

S

S

E

E

I

V

T

T

G

A

P

P

T

H

V

F

G

S

A

A

D

D

R

K

V

F

Q

G

E

P

K

K

D

D

Y

N

D

D

L

L

T

I

A

L

Q

N

H

Y

A

A

-

K

-

D

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

V

I

I

I

V

H

H

G

G

R

Y

V

V

L

R

D

D

E

Q

H

F

G

G

Q

R

P

P

V

V

P

K

G

N

I

A

L

L

V

V

E

E

I

V

W

W

Q

Q

A

A

N

N

A

A

A

S

G

G

R

R

Y
|
Y

N

R

H

H

D

P

R

N

D

D

N

Q

H

Y

D

I

A

G

P

A

L

M

D

D

P

P

N

N

F

F

T

G

G

G

T

C

G

G

R

R

T

M

V

L

T

T

D

D

A

D

D

N

G

G

W

Y

Y

Y

Q

V

F

F

Q

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W

W

G

R

N

N

H

R

H

I

N

N

A

E

W

W

R
|
R

P

P

A

A

H
|
H

I

I

H
|
H

F

F

S

S

L

L

F

I

G

A

P

D

S

G

I

W

L

A

T

Q

R

R

L

L

V

I

T

S

Q

Q

M

F

Y

Y

F

F

P

E

G

G

D

D

P

T

L

L

L

I

E

D

Y

S

D

C

P

P

I

I

Y

L

N

K

C

T

V

I

P

P

D

S

T

E

S

Q

A

Q

K

R

E

R

R

A

L

L

I

I

S

A

S

L

F

E

D

D

L

K

E

S

K

N

T

F

I

I

P

E

N

A

Y

D

A

S

L

R

G

C

Y

Y

R

R

W

F

D

D

I

I

V

T

L

L

R

R

G

G

R

R

D

R

A

A

T

T

P

Y

M

F

E

E

active site: Y106 (= Y105), Y145 (= Y144), R155 (= R154), H158 (= H157), H160 (= H159)
binding catechol: Y106 (= Y105), R155 (= R154), H158 (= H157)
binding fe (iii) ion: Y106 (= Y105), H158 (= H157), H160 (= H159)

2bumB Crystal structure of wild-type protocatechuate 3,4-dioxygenase from acinetobacter sp. Adp1 (see paper)
48% identity, 91% coverage: 21:233/234 of query aligns to 20:234/238 of 2bumB

query
sites
2bumB

P

P

P

G

Y

Y

Q

K

S

T

T

S

N

V

L

L

R

R

S

S

P

P

S

K

K

N

P

A

L

L

V

I

F

S

L

I

P

A

H

E

S

T

L

L

S

S

E

E

I

V

T

T

G

A

P

P

T

H

V

F

G

S

A

A

D

D

R

K

V

F

Q

G

E

P

K

K

D

D

Y

N

D

D

L

L

T

I

A

L

Q

N

H

Y

A

A

-

K

-

D

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

V

I

I

I

V

H

H

G

G

R

Y

V

V

L

R

D

D

E

Q

H

F

G

G

Q

R

P

P

V

V

P

K

G

N

I

A

L

L

V

V

E

E

I

V

W

W

Q

Q

A

A

N

N

A

A

A

S

G

G

R

R

Y
|
Y

N

R

H

H

D

P

R

N

D

D

N

Q

H

Y

D

I

A

G

P

A

L

M

D

D

P

P

N

N

F

F

T

G

G

G

T

C

G

G

R

R

T

M

V

L

T

T

D

D

A

D

D

N

G

G

W

Y

Y

Y

Q

V

F

F

Q

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W

W

G

R

N

N

H

R

H

I

N

N

A

E

W

W

R
|
R

P

P

A

A

H
|
H

I

I

H
|
H

F

F

S

S

L

L

F

I

G

A

P

D

S

G

I

W

L

A

T

Q

R

R

L

L

V

I

T

S

Q

Q

M

F

Y

Y

F

F

P

E

G

G

D

D

P

T

L

L

L

I

E

D

Y

S

D

C

P

P

I

I

Y

L

N

K

C

T

V

I

P

P

D

S

T

E

S

Q

A

Q

K

R

E

R

R

A

L

L

I

I

S

A

S

L

F

E

D

D

L

K

E

S

K

N

T

F

I

I

P

E

N

A

Y

D

A

S

L

R

G

C

Y

Y

R

R

W

F

D

D

I

I

V

T

L

L

R

R

G

G

R

R

D

R

A

A

T

T

P

Y

M

F

E

E

active site: Y106 (= Y105), Y145 (= Y144), R155 (= R154), H158 (= H157), H160 (= H159)
binding fe (iii) ion: Y106 (= Y105), Y145 (= Y144), H158 (= H157), H160 (= H159)

1eocB Crystal structure of acinetobacter sp. Adp1 protocatechuate 3,4- dioxygenase in complex with 4-nitrocatechol (see paper)
48% identity, 91% coverage: 21:233/234 of query aligns to 20:234/238 of 1eocB

query
sites
1eocB

P

P

P

G

Y
|
Y

Q

K

S

T

T

S

N

V

L

L

R

R

S

S

P

P

S

K

K

N

P

A

L

L

V

I

F

S

L

I

P

A

H

E

S

T

L

L

S

S

E

E

I

V

T

T

G

A

P

P

T

H

V

F

G

S

A

A

D

D

R

K

V

F

Q

G

E

P

K

K

D

D

Y

N

D

D

L

L

T

I

A

L

Q

N

H

Y

A

A

-

K

-

D

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

V

I

I

I

V

H

H

G

G

R

Y

V

V

L

R

D

D

E

Q

H

F

G

G

Q

R

P

P

V

V

P

K

G

N

I

A

L

L

V

V

E

E

I

V

W

W

Q

Q

A

A

N

N

A

A

A

S

G

G

R

R

Y
|
Y

N

R

H

H

D

P

R

N

D

D

N

Q

H

Y

D

I

A

G

P

A

L

M

D

D

P

P

N

N

F

F

T

G

G

G

T

C

G

G

R

R

T

M

V

L

T

T

D

D

A

D

D

N

G

G

W

Y

Y

Y

Q

V

F

F

Q

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W
|
W

G

R

N

N

H

R

H

I

N

N

A

E

W

W

R
|
R

P

P

A

A

H
|
H

I

I

H
|
H

F

F

S

S

L

L

F

I

G

A

P

D

S

G

I

W

L

A

T

Q

R

R

L

L

V

I

T

S

Q

Q

M

F

Y

Y

F

F

P

E

G

G

D

D

P

T

L

L

L

I

E

D

Y

S

D

C

P

P

I
|
I

Y

L

N

K

C

T

V

I

P

P

D

S

T

E

S

Q

A

Q

K

R

E

R

R

A

L

L

I

I

S

A

S

L

F

E

D

D

L

K

E

S

K

N

T

F

I

I

P

E

N

A

Y

D

A

S

L

R

G

C

Y

Y

R

R

W

F

D

D

I

I

V

T

L

L

R

R

G

G

R

R

D

R

A

A

T

T

P

Y

M

F

E

E

active site: Y106 (= Y105), Y145 (= Y144), R155 (= R154), H158 (= H157), H160 (= H159)
binding 4-nitrocatechol: Y22 (= Y23), Y106 (= Y105), W147 (= W146), R155 (= R154), H158 (= H157), H160 (= H159), I189 (= I188)
binding fe (iii) ion: Y106 (= Y105), H158 (= H157), H160 (= H159)

Query Sequence

>AO356_04925 FitnessBrowser__pseudo5_N2C3_1:AO356_04925
MTDKPGYRRPQAGTQPEYLHPPYQSTNLRSPSKPLVFLPHSLSEITGPTVGADRVQEKDY
DLTAQHAGEPLGERIIIHGRVLDEHGQPVPGILVEIWQANAAGRYNHDRDNHDAPLDPNF
TGTGRTVTDADGWYQFQTIKPGAYPWGNHHNAWRPAHIHFSLFGPSILTRLVTQMYFPGD
PLLEYDPIYNCVPDTSAKERLISSFDLEKTIPNYALGYRWDIVLRGRDATPMEK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory