SitesBLAST
Comparing AO356_04945 FitnessBrowser__pseudo5_N2C3_1:AO356_04945 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
89% identity, 100% coverage: 1:448/448 of query aligns to 1:448/448 of Q51955
- D41 (= D41) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
- D44 (= D44) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
- G85 (= G85) mutation to V: Abolishes 4-HBA transport and chemotaxis.
- D89 (= D89) mutation to N: Abolishes 4-HBA transport and chemotaxis.
- G92 (= G92) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
- R124 (= R124) mutation to A: Abolishes 4-HBA transport.
- E144 (= E144) mutation to A: Strong decrease in 4-HBA transport.
- H183 (= H183) mutation to A: Decrease in 4-HBA transport and chemotaxis.
- D323 (= D323) mutation to N: Abolishes 4-HBA transport and chemotaxis.
- H328 (= H328) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to R: Decrease in 4-HBA transport and loss of chemotaxis.
- R386 (= R386) mutation to A: Strong decrease in 4-HBA transport.
- R398 (= R398) mutation to A: Abolishes 4-HBA transport.
- H444 (= H444) mutation to A: No change in 4-HBA transport and chemotaxis.
Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
43% identity, 95% coverage: 12:438/448 of query aligns to 5:429/452 of Q5EXK5
- D82 (= D89) mutation to A: Loss of activity.
- V311 (≠ W320) mutation to W: Loss of activity.
- D314 (= D323) mutation to A: Loss of activity.
P77589 3-(3-hydroxy-phenyl)propionate transporter; 3HPP transporter; 3-(3-hydroxy-phenyl)propionate:H(+) symporter; 3HPP:H(+) symporter from Escherichia coli (strain K12) (see paper)
36% identity, 88% coverage: 33:424/448 of query aligns to 19:375/403 of P77589
- E27 (≠ D41) mutation to A: Lack of 3HPP transport activity.; mutation to D: Slight decrease in 3HPP transport activity.
- D75 (= D89) mutation D->A,E: Lack of 3HPP transport activity.
- A272 (≠ W320) mutation to H: 30% increase in 3HPP transport activity.
- K276 (≠ R324) mutation to D: Lack of 3HPP transport activity.
Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
27% identity, 88% coverage: 23:418/448 of query aligns to 36:404/444 of Q8NLB7
- D54 (= D41) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
- D57 (= D44) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
- R103 (= R90) mutation to A: Loss of transport activity.
- W309 (= W320) mutation to V: Loss of transport activity.
- D312 (= D323) mutation to A: Loss of transport activity.
- R313 (= R324) mutation to A: Loss of transport activity.
- I317 (≠ H328) mutation I->H,Y: Loss of transport activity.
- R386 (= R398) mutation to A: Loss of transport activity.
8uo8A Synaptic vesicle glycoprotein 2B (see paper)
26% identity, 63% coverage: 25:305/448 of query aligns to 20:324/574 of 8uo8A
- binding [(2R)-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-2-propanoyloxy-propyl] propanoate: M167 (vs. gap), I171 (≠ L176), Y185 (vs. gap), S189 (= S184), V192 (≠ L185)
- binding (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en: E148 (≠ L153), T159 (≠ A167), S170 (≠ K175)
- binding 1,2-didecanoyl-sn-glycero-3-[phospho-l-serine]: W23 (= W28), F27 (≠ I32)
- binding (4R)-4-(2-chloro-2,2-difluoroethyl)-1-{[(4R)-2-(methoxymethyl)-6-(trifluoromethyl)imidazo[2,1-b][1,3,4]thiadiazol-5-yl]methyl}pyrrolidin-2-one: F134 (≠ T139), W157 (vs. gap), W288 (≠ Y272), Y295 (≠ V279), Y296 (= Y280)
Sites not aligning to the query:
- binding (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en: 449
- binding 1,2-didecanoyl-sn-glycero-3-[phospho-l-serine]: 18, 19
- binding (4R)-4-(2-chloro-2,2-difluoroethyl)-1-{[(4R)-2-(methoxymethyl)-6-(trifluoromethyl)imidazo[2,1-b][1,3,4]thiadiazol-5-yl]methyl}pyrrolidin-2-one: 440, 494, 495, 498, 502, 522, 525, 526
- binding cholesterol hemisuccinate: 442, 443, 462, 492, 493
A0A0H2VG78 Glucose transporter GlcP; Glucose/H(+) symporter from Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) (see paper)
30% identity, 41% coverage: 41:225/448 of query aligns to 23:201/446 of A0A0H2VG78
- R102 (= R124) mutation to A: Loss of transport activity.
- I105 (≠ T127) mutation to S: Affects symport activity. May function as an uniporter.
- E122 (= E144) mutation to A: Loss of transport activity.
- Q137 (≠ F159) mutation to A: Loss of transport activity.
Sites not aligning to the query:
- 22 D→N: Affects symport activity. May function as an uniporter.
- 250 Q→A: Loss of transport activity.
- 251 Q→A: Loss of transport activity.
- 256 N→A: Loss of transport activity.
- 357 W→A: Loss of transport activity.
9b1lA Urate bound human urat1 in the outward-facing state. (see paper)
31% identity, 40% coverage: 62:241/448 of query aligns to 135:313/529 of 9b1lA
Sites not aligning to the query:
9b1mA Pyrazinoate bound human urat1 in the inward-facing state (site1) (see paper)
31% identity, 40% coverage: 62:241/448 of query aligns to 135:313/515 of 9b1mA
Sites not aligning to the query:
9b1iA Solute carrier family 22 member 12 (see paper)
31% identity, 40% coverage: 62:241/448 of query aligns to 135:313/515 of 9b1iA
Sites not aligning to the query:
9b1hA Solute carrier family 22 member 12 (see paper)
31% identity, 40% coverage: 62:241/448 of query aligns to 135:313/515 of 9b1hA
Sites not aligning to the query:
9b1gA Solute carrier family 22 member 12 (see paper)
31% identity, 40% coverage: 62:241/448 of query aligns to 135:313/515 of 9b1gA
Sites not aligning to the query:
O76082 Organic cation/carnitine transporter 2; High-affinity sodium-dependent carnitine cotransporter; Solute carrier family 22 member 5 from Homo sapiens (Human) (see 21 papers)
26% identity, 78% coverage: 56:406/448 of query aligns to 137:479/557 of O76082
- A142 (= A64) to S: in CDSP; uncertain significance; reduces carnitine transport but the mutant retains more than 25% of wild-type activity; dbSNP:rs151231558
- P143 (≠ S65) to L: in CDSP; carnitine transport reduced to less than 2% of wild-type; dbSNP:rs1178584184
- L144 (= L66) to F: in dbSNP:rs10040427
- V151 (≠ I75) to M: in CDSP; uncertain significance; reduces carnitine transport but the mutant retains more than 60% of wild-type activity; dbSNP:rs386134193
- R169 (= R93) to P: in CDSP; loss of carnitine transport; dbSNP:rs121908889; to Q: in CDSP; loss of carnitine transport; dbSNP:rs121908889; to W: in CDSP; loss of carnitine transport; dbSNP:rs121908890
- V175 (≠ G99) to M: in CDSP; carnitine transport reduced to less than 10% of wild-type; dbSNP:rs781721860
- M177 (≠ V101) to V: in CDSP; carnitine transport reduced to less than 20% of wild-type; dbSNP:rs145068530
- M179 (≠ L103) to L: in CDSP; uncertain significance; reduces carnitine transport but the mutant retains more than 40% of wild-type activity; dbSNP:rs386134196
- L186 (≠ A110) to P: in CDSP; loss of carnitine transport; dbSNP:rs386134197
- M205 (≠ L129) to R: in CDSP; loss of carnitine transport; dbSNP:rs796052033
- N210 (≠ G134) to S: in CDSP; loss of carnitine transport; dbSNP:rs386134198
- Y211 (≠ M135) to C: in CDSP; loss of carnitine transport; dbSNP:rs121908888
- A214 (= A138) to V: in CDSP; uncertain significance; reduces carnitine transport but the mutant retains 30% of wild-type activity; dbSNP:rs386134199
- T219 (≠ S143) to K: in CDSP; uncertain significance; reduces carnitine transport but the mutant retains 30% of wild-type activity
- S225 (≠ R149) to L: in CDSP; reduces carnitine transport to less than 20% of wild-type activity; dbSNP:rs386134205
- R227 (≠ K151) to H: in CDSP; reduces carnitine transport to less than 10% of wild-type activity; dbSNP:rs185551386
- F230 (≠ L154) to L: in CDSP; reduces carnitine transport to less than 1% of wild-type activity; dbSNP:rs756650860
- S231 (≠ V155) to F: in CDSP; loss of carnitine transport; dbSNP:rs386134206
- T232 (= T156) to M: in CDSP; reduces carnitine transport to less than 20% of wild-type activity; dbSNP:rs114269482
- A240 (≠ G168) to T: in CDSP; reduces carnitine transport to less than 2% of wild-type activity
- G242 (= G170) to V: in CDSP; loss of carnitine transport; dbSNP:rs72552728
- P247 (≠ K175) to R: in CDSP; loss of carnitine transport; dbSNP:rs2126783802
- R254 (vs. gap) to Q: in CDSP; uncertain significance; reduces carnitine transport but the mutant retains more than 30% of wild-type activity; dbSNP:rs200699819
- R257 (≠ H183) to W: in CDSP; reduces carnitine transport to less than 10% of wild-type activity; dbSNP:rs386134203
- T264 (≠ I191) to M: in CDSP; uncertain significance; reduces carnitine transport but the mutant retains more than 40% of wild-type activity; dbSNP:rs201262157; to R: in CDSP; reduces carnitine transport to less than 5% of wild-type activity; dbSNP:rs201262157
- L269 (= L196) to P: in CDSP; uncertain significance; reduces carnitine transport but the mutant retains more than 40% of wild-type activity; dbSNP:rs1752496410
- S280 (= S207) to F: in CDSP; reduces carnitine transport to less than 1% of wild-type activity; dbSNP:rs386134208
- R282 (= R209) to Q: in CDSP; reduces carnitine transport to 5% of wild-type activity; dbSNP:rs386134210
- W283 (≠ Y210) to C: in CDSP; loss of carnitine transport; dbSNP:rs386134211; to R: in CDSP; reduces carnitine transport to less than 1% of wild-type activity; dbSNP:rs72552729
- A301 (≠ R222) to D: in CDSP; reduces carnitine transport to less-than-1% to 3% of wild-type activity; dbSNP:rs72552730
- I312 (≠ V233) to V: in CDSP; uncertain significance; reduces carnitine transport but the mutant retains 70% of wild-type activity; dbSNP:rs77300588
- E317 (≠ S238) to K: in CDSP; uncertain significance; no effect on carnitine transport; dbSNP:rs774792831
- I348 (≠ L266) to T: in CDSP; uncertain significance; reduces carnitine transport but the mutant retains 60% of wild-type activity; dbSNP:rs150544263
- W351 (= W269) to R: in CDSP; loss of carnitine transport; dbSNP:rs68018207
- M352 (≠ L270) mutation to R: Loss of both carnitine and organic cation transport functionalities. No effect on protein expression.
- S355 (≠ F273) to L: in CDSP; reduces carnitine transport to less than 2% of wild-type activity; dbSNP:rs1385634398
- Y358 (≠ L276) to N: in CDSP; loss of carnitine transport; dbSNP:rs61731073
- L363 (= L281) to P: in CDSP; loss of carnitine transport; dbSNP:rs386134214
- L394 (≠ D323) natural variant: Missing (in CDSP; reduces carnitine transport to 5% of wild-type activity)
- P398 (= P327) to L: in CDSP; reduces carnitine transport to less than 1% of wild-type activity; dbSNP:rs144547521
- R399 (≠ H328) to Q: in CDSP; carnitine transport is reduced to less than 1% of normal; dbSNP:rs121908891; to W: in CDSP; reduces carnitine transport to less than 5% of wild-type activity; dbSNP:rs267607054
- S412 (vs. gap) to G: in CDSP; uncertain significance; no effect on carnitine transport
- V439 (= V366) to G: in CDSP; reduces carnitine transport to less than 1% of wild-type activity
- T440 (≠ N367) to M: in CDSP; loss of carnitine transport; dbSNP:rs72552732
- A442 (= A369) to I: in CDSP; reduces carnitine transport to less than 20% of wild-type activity; requires 2 nucleotide substitutions; dbSNP:rs267607053
- F443 (≠ Q370) to V: in CDSP; reduces carnitine transport to less than 1% of wild-type
- V446 (≠ M373) to F: in CDSP; reduces carnitine transport to less than 1% of wild-type; dbSNP:rs72552733
- Y447 (≠ P374) to C: in CDSP; loss of carnitine transport; dbSNP:rs386134218
- V448 (≠ S375) to L: in CDSP; reduces carnitine transport to less than 20% of wild-type; dbSNP:rs386134219
- Y449 (≠ L376) to D: in CDSP; uncertain significance; reduces carnitine transport to less than 20% of wild-type; dbSNP:rs11568514
- E452 (≠ R379) to K: in CDSP; reduces carnitine transport to less than 5% of wild-type; dbSNP:rs72552734
- P455 (= P382) to R: in CDSP; loss of carnitine transport; dbSNP:rs1408166345
- G462 (= G389) to V: in CDSP; reduces carnitine transport to less than 5% of wild-type
- S467 (≠ L394) to C: in CDSP; reduces carnitine transport to less than 20% of wild-type activity; dbSNP:rs60376624
- T468 (≠ G395) to R: in CDSP; markedly reduced carnitine transport compared to the wild-type protein; less than 1% of wild-type activity; dbSNP:rs386134221
- S470 (≠ G397) to F: in CDSP; loss of carnitine transport; dbSNP:rs386134222
- R471 (= R398) to H: in CDSP; reduces carnitine transport to less than 2% of wild-type; dbSNP:rs386134223; to P: in CDSP; loss of carnitine transport; dbSNP:rs386134223
- L476 (= L403) to R: in CDSP; loss of carnitine transport
- P478 (≠ A405) to L: in CDSP; loss of carnitine transport but stimulated organic cation transport; no effect on protein expression; dbSNP:rs72552735
Sites not aligning to the query:
- 12 G → S: in CDSP; uncertain significance; reduces carnitine transport but the mutant retains 50% of wild-type activity; dbSNP:rs139203363
- 15 G → W: in CDSP; carnitine transport reduced to less than 20% of wild-type; dbSNP:rs267607052
- 16 P → L: in CDSP; loss of carnitine transport
- 17 F → L: in CDSP; carnitine transport reduced to less than 20% of wild-type; dbSNP:rs11568520
- 19 R → P: in CDSP; carnitine transport is reduced to less than 5% of normal; dbSNP:rs72552723
- 20 L → H: in CDSP; uncertain significance; reduces carnitine transport but the mutant retains 50% of wild-type activity; dbSNP:rs144020613
- 22 natural variant: Missing (in CDSP; reduces carnitine transport to less than 1% of normal)
- 26 S → N: in CDSP; carnitine transport reduced to less than 6% of wild-type; dbSNP:rs772578415
- 28 S → I: in CDSP; carnitine transport reduced to 1% of wild-type; dbSNP:rs72552724
- 32 N → S: in CDSP; carnitine transport reduced to less than 1% of wild-type; dbSNP:rs72552725
- 44 A → V: in CDSP; carnitine transport reduced to less than 10% of wild-type; dbSNP:rs199689597
- 46 P → L: in CDSP; carnitine transport reduced to less than 5% of wild-type; dbSNP:rs377767445; P → S: in CDSP; carnitine transport is reduced to less than 5% of normal; dbSNP:rs202088921
- 50 C → Y: in CDSP; loss of carnitine transport
- 57 modified: carbohydrate, N-linked (GlcNAc...) asparagine
- 66 T → P: in CDSP; carnitine transport reduced to 2% of wild-type; dbSNP:rs1476076948
- 75 R → P: in CDSP; carnitine transport reduced to 2% of wild-type; dbSNP:rs757711838
- 83 R → L: in CDSP; loss of carnitine transport; dbSNP:rs72552726
- 91 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→Q: Reduces expression to 50%. No effect on carnitine transporter activity.
- 93 S → W: in CDSP; loss of carnitine transport; dbSNP:rs386134190
- 95 L → V: in CDSP; uncertain significance; reduces carnitine transport but the mutant retains 30% of wild-type activity; dbSNP:rs386134191
- 96 G → A: in CDSP; carnitine transport reduced to 20% of wild-type; dbSNP:rs377767450
- 115 D → G: in CDSP; carnitine transport reduced to less than 5% of wild-type; dbSNP:rs386134192
- 123 V → G: in CDSP; carnitine transport reduced to less than 20% of wild-type; dbSNP:rs748605096
- 131 E → D: in CDSP; uncertain significance; may affect splicing; reduces carnitine transport but the mutant retains 30% of wild-type activity
- 481 V → F: reduces carnitine transport but the mutant retains more than 60% of wild-type activity; dbSNP:rs11568513; V → I: in dbSNP:rs11568513
- 488 R → C: in CDSP; reduces carnitine transport to less than 10% of wild-type; dbSNP:rs377216516; R → H: in CDSP; uncertain significance; reduces carnitine transport to 40% of wild-type; dbSNP:rs28383481
- 507 L → S: in CDSP; reduces carnitine transport to 5% of wild-type; dbSNP:rs1157198543
- 508 F → L: in dbSNP:rs11568521
- 530 M → V: in dbSNP:rs11568524
- 549 P → S: reduces carnitine transport but the mutant retains more than 20% of wild-type activity; dbSNP:rs11568525
8k77A Synaptic vesicle glycoprotein 2A (see paper)
25% identity, 60% coverage: 25:292/448 of query aligns to 27:324/592 of 8k77A
Sites not aligning to the query:
8jlhA Cryo-em structure of sv2a dimer in complex with bont/a2 hc and levetiracetam (see paper)
25% identity, 60% coverage: 25:292/448 of query aligns to 27:324/592 of 8jlhA
Sites not aligning to the query:
8jlcA Synaptic vesicle glycoprotein 2A (see paper)
25% identity, 60% coverage: 25:292/448 of query aligns to 27:324/592 of 8jlcA
Sites not aligning to the query:
Q9Z2I6 Synaptic vesicle glycoprotein 2C; Synaptic vesicle protein 2C from Rattus norvegicus (Rat) (see 3 papers)
28% identity, 53% coverage: 25:260/448 of query aligns to 149:399/727 of Q9Z2I6