SitesBLAST

D57 (= D35) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (= R88) mutation to A: Loss of transport activity.
W309 (= W304) mutation to V: Loss of transport activity.
D312 (= D307) mutation to A: Loss of transport activity.
R313 (= R308) mutation to A: Loss of transport activity.

Sites not aligning to the query:

54 D→A: Loss of transport activity.; D→E: Retains 50% of its transport activity.
317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

O15245 Solute carrier family 22 member 1; Organic cation transporter 1; hOCT1 from Homo sapiens (Human) (see 10 papers)
25% identity, 83% coverage: 35:398/437 of query aligns to 123:487/554 of O15245

query
sites
O15245

D

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L160 (≠ A71) to F: no changes in both MPP(+) and TEA uptake; abolishes MPP(+) uptake when associated with S-401; largely localized to the plasma membrane; dbSNP:rs683369
S189 (≠ C105) to L: no changes in MPP(+) uptake; dbSNP:rs34104736
G220 (≠ A144) to V: affects transporter activity; reduction of MPP(+) uptake when associated with V-408; dbSNP:rs36103319
Y240 (≠ P164) mutation to F: Decreased TEA uptake.
P283 (= P199) to L: in dbSNP:rs4646277; mutation to A: Decreased TEA uptake.
R287 (vs. gap) to G: in dbSNP:rs4646278
P341 (≠ T248) to L: affects transporter activity; reduction of TEA uptake; reduction o MPP(+) uptake when associated with V-408; largely localized to the plasma membrane; dbSNP:rs2282143
R342 (≠ L249) to H: no changes in MPP(+) uptake when associated with V-408; dbSNP:rs34205214
Y361 (≠ F268) mutation to F: Decreased TEA uptake.
Y376 (≠ F284) mutation to F: Decreased TEA uptake.
G401 (= G310) to S: affects transporter activity; reduction of MPP(+), serotonin and TEA uptake; no MPP(+) uptake when associated with L-160; dbSNP:rs34130495
M408 (≠ I317) to V: does not affect transporter activity; no changes in MPP(+) uptake when associated with F-14; no changes in MPP(+) uptake when associated with F-85; no changes in MPP(+) uptake when associated with L-189; no changes in MPP(+) uptake when associated with H-342; no changes in MPP(+) uptake when associated with M-420 del; no changes in MPP(+) uptake when associated with I-440; no changes in MPP(+) uptake when associated with I-461; no changes in MPP(+) uptake when associated with M-488; reduction of MPP uptake when associated with C-61; no MPP(+) uptake when associated with V-220; reduction of MPP(+) uptake when associated with L-341; no MPP(+) uptake when associated with S-401; no MPP(+) uptake when associated with R-465; dbSNP:rs628031
M420 (= M329) natural variant: Missing (reduction of serum O-isobutanoyl-(R)-carnitine levels; no change in MPP(+) uptake; no changes in MPP(+) uptake when associated with V-408; dbSNP:rs72552763)
M440 (= M352) to I: in dbSNP:rs35956182
V461 (= V371) to I: no changes in MPP(+) uptake when associated with V-408; dbSNP:rs34295611
G465 (= G375) to R: reduction of the localization to the basolateral membrane; no MPP(+) uptake when associated with V-408; dbSNP:rs34059508; mutation to A: No changes in MPP(+) uptake.

Sites not aligning to the query:

14 S → F: exclusively found in the African American population; increased MPP(+) uptake when associated with V-408; dbSNP:rs34447885
24 I→L: No change in fenoterol uptake. No change in trospium uptake. No change in terbutaline uptake.
28 L→I: No change in fenoterol uptake. No change in trospium uptake. No change in terbutaline uptake.
31 A→S: No change in fenoterol uptake. No change in trospium uptake. No change in terbutaline uptake.
32 F→L: No change in fenoterol uptake. Decreased trospium uptake. Decreased trospium affinity.
36 C→Y: Increased fenoterol uptake. Increased fenoterol affinity. No change in trospium uptake. No change in terbutaline uptake. No change in terbutaline affinity.
41 F → L: in dbSNP:rs2297373
61 R → C: affects transporter activity; reduction of MPP(+) uptake; reduction of serum O-isobutanoyl-(R)-carnitine levels; reduction of MPP(+) uptake when associated with V-408; dbSNP:rs12208357
85 L → F: no changes in MPP(+) uptake; when associated with V-408; dbSNP:rs35546288
88 C → R: affects transporter activity; reduction of MPP(+), serotonin and TEA uptake; dbSNP:rs55918055
488 R → M: no changes in MPP(+) uptake when associated with V-408; dbSNP:rs35270274

8et8A Cryo-em structure of the organic cation transporter 1 in complex with verapamil (see paper)
24% identity, 74% coverage: 77:401/437 of query aligns to 160:489/532 of 8et8A

query
sites
8et8A

L

L

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binding (2S)-2-(3,4-dimethoxyphenyl)-5-{[2-(3,4-dimethoxyphenyl)ethyl](methyl)amino}-2-(propan-2-yl)pentanenitrile: K213 (≠ G138), W216 (= W141), Q240 (= Q165), W353 (≠ L261), Y360 (≠ F268), F378 (≠ E282), S381 (≠ L285), E385 (≠ C289), C449 (vs. gap), S469 (= S377)

Sites not aligning to the query:

binding (2S)-2-(3,4-dimethoxyphenyl)-5-{[2-(3,4-dimethoxyphenyl)ethyl](methyl)amino}-2-(propan-2-yl)pentanenitrile: 31, 35

8et7A Cryo-em structure of the organic cation transporter 1 in complex with diphenhydramine (see paper)
24% identity, 74% coverage: 77:401/437 of query aligns to 160:489/532 of 8et7A

query
sites
8et7A

L

L

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N

T

L

G

G

-

V

-

M

-

V

A

C

S

S

A

S

A

L

Y

C

N

D

L

L

G

G

I

I

V

I

G

-

A

-

S

-

A

-

A

T

P

P

F

F

F

L

A

V

Q

F

K

R

L

L

V

M

A

E

M

V

binding n-[2-(benzhydryloxy)ethyl]-n,n-dimethylamine: E385 (≠ C289), F445 (vs. gap)

Sites not aligning to the query:

binding n-[2-(benzhydryloxy)ethyl]-n,n-dimethylamine: 35

8sc2A Human oct1 bound to diltiazem in inward-open conformation (see paper)
29% identity, 38% coverage: 35:199/437 of query aligns to 105:265/453 of 8sc2A

query
sites
8sc2A

D

D

F

G

Y

W

V

V

Y

Y

A

D

T

T

A

P

A

G

A

S

L

S

V

I

I

V

G

T

P

E

V

F

-

N

F

L

F

V

P

C

Q

A

T

D

S

S

G

W

T

K

A

L

Q

D

M

L

L

F

S

Q

S

S

F

C

L

L

T

N

F

A

G

G

I

F

A

L

F

F

L

-

A

-

R

-

P

-

L

-

G

G

S

S

A

L

L

G

F

V

G

G

H

Y

F

F

G

A

D

D

R

R

I

F

G

G

R

R

K

K

S

L

T

C

L

L

V

L

A

G

S

T

L

V

L

L

L

V

M

N

G

A

V

V

C

S

T

G

T

V

L

L

I

M

G

A

V

F

L

S

P

P

G

N

Y

Y

A

M

S

S

I

-

G

-

A

-

W

-

A

-

P

-

I

-

L

-

L

M

C

L

L

L

L

F

R

R

F

L

G

L

Q

Q

G

G

L

L

G

V

L

S

G

K

G

G

E

N

W
|
W

G

M

G

A

A

G

A

Y

L

T

L

L

A

I

T

T

E

E

N

F

A

V

P

G

K

S

G

G

K

S

R

R

A

R

W

T

F

V

G

A

M

I

F

M

P

Y

Q
|
Q

L

M

G

A

P
x
F

S

T

I

V

G

G

F

L

L

V

A

A

A

L

N

T

G

G

L

L

-

A

-

Y

-

A

-

L

-

P

-

H

-

W

-

R

F

W

L

L

T

Q

L

L

A

A

M

V

S

S

L

L

D

P

D

T

E

F

Q

L

F

F

R

L

D

L

W

Y

G

Y

W

W

R

C

I

V

P

P

binding Diltiazem: W199 (= W141), Q223 (= Q165), F226 (≠ P168)

Sites not aligning to the query:

binding Diltiazem: 18, 293, 297, 385

O08966 Solute carrier family 22 member 1; Organic cation transporter 1; mOCT1 from Mus musculus (Mouse) (see paper)
24% identity, 74% coverage: 77:401/437 of query aligns to 162:491/556 of O08966

query
sites
O08966

L

L

G

G

S

S

A

L

L

V

F

V

G

G

H

Y

F

I

G

A

D

D

R

R

I

F

G

G

R

R

K

K

S

L

T

C

L

L

V

L

A

V

S

T

L

T

L

L

L

V

M

T

G

S

V

L

C

S

T

G

T

V

L

L

I

T

G

A

V

V

L

A

P

P

G

D

Y

Y

A

T

S

S

I

-

G

-

A

-

W

-

A

-

P

-

I

-

L

-

L

M

C

L

L

L

L

F

R

R

F

L

G

L

Q

Q

G

G

L

M

G

V

L

S

G

K

G

G

E

S

W

W

G

V

G

S

A

G

A

Y

L

T

L

L

A

I

T

T

E

E

N

F

A

V

P

G

K

S

G

G

K

Y

R

R

A

R

W

T

F

T

G

A

M

I

F

L

P

Y

Q

Q

L

V

G

A

P

F

S

T

I

V

G

G

F

L

L

V

A

G

A

L

N

A

G

G

L

V

-

A

-

Y

-

A

-

I

-

P

-

D

-

W

-

R

F

W

L

L

T

Q

L

L

A

A

M

V

S

S

L

L

D

P

D

T

E

F

Q

L

F

F

R

L

D

L

W

Y

G

Y

W

W

R

F

I

V

P

P

-

E

-

S

-

P

-

R

F

W

L

L

S

S

A

Q

V

K

L

R

V

T

I

T

V

Q

G

A

L

V

Y

R

V

I

R

M

L

E

K

Q

L

I

H

A

E

Q

T

K

-

N

-

R

-

K

-

V

-

P

P

P

V

A

F

D

A

L

N

K

A

M

M

M

A

C

R

L

Q

E

E

E

R

D

V

A

K

S

V

E

P

R

L

R

V

S

E

P

L

S

F

F

S

A

Q

D

Y

L

W

F

A

R

P

T

T

P

L

S

L

L

G

R

A

K

A

H

A

T

M

L

V

I

V

L

C

M

Y

Y

A

L

L

W

F

F

Y

S

I

C

S

A

T

V

V

L

F

Y

S

Q

-

G

L

L

S

I

Y

M

G

H

V

V

S

G

T

A

L

T

G

G

-

A

-

N

-

L

Y

Y

S

L

R

D

E

F

T

F

F

Y

L

S

G

S

L

L

L

V

C

E

F

F

A

P

V

A

L

A

F

F

M

I

A

I

A

L

A

V

T

T

P

-

L

-

S

-

A

-

W

-

A

-

S

I

D

D

R

R

Y

I

G

G

R

R

K

I

P

Y

I

P

L

I

I

A

G

S

G

N

L

L

L

V

A

A

I

G

A

A

S

A

G

C

F

L

L

L

M

M

E

-

P

-

L

-

T

-

H

-

G

-

S

-

T

-

G

-

V

-

A

-

L

I

F

F

L

I

C

P

I

H

E

E

L

L

F

H

L

W

M

L

G

N

V

V

T

T

F

L

A

A

P

C

M

L

G

G

A

R

L

M

-

G

-

A

-

T

-

I

-

V

-

L

-

Q

-

M

-

V

-

C

-

L

-

V

L

N

P

A

E

E

L

L

F

Y

P

P

T

T

H

F

V

I

R

R

Y

N

T

L

G

G

-

M

-

V

A

C

S

S

A

A

A

L

Y

C

N

D

L

L

G

G

I

I

V

F

G

-

A

-

S

-

A

-

A

T

P

P

F

F

F

M

A

V

Q

F

K

R

L

L

V

M

A

E

M

V

Sites not aligning to the query:

32 L→F: Increased trospium uptake. Increased trospium affinity. No change in fenoterol uptake.
36 Y→C: Decreased fenoterol uptake. Decreased fenoterol affinity. No change in trospium uptake. No change in terbutaline affinity.

Q9R0W2 Solute carrier family 22 member 2; Organic cation transporter 2; rOCT2 from Rattus norvegicus (Rat) (see paper)
23% identity, 74% coverage: 77:401/437 of query aligns to 162:491/555 of Q9R0W2

query
sites
Q9R0W2

L

I

G

G

S

A

A

M

L

M

F

I

G

G

H

Y

F

L

G

A

D

D

R

R

I

F

G

G

R

R

K

K

S

F

T

C

L

L

V

L

A

V

S

T

L

I

L

L

L

I

M

N

G

A

V

I

C

S

T

G

T

A

L

L

I

M

G

A

V

I

L

S

P

P

G

N

Y

Y

A

-

S

-

I

-

G

-

A

A

W

W

A

-

P

-

I

-

L

-

L

M

C

L

L

V

L

F

R

R

F

F

G

L

Q

Q

G

G

L

L

G

V

L

S

G

K

G

A

E

G

W

W

G

L

G

I

A

G

A

Y

L

I

L

L

A

I

T

T

E

E

N

F

A

V

P

G

K

L

G

G

K

Y

R

R

A

R

W

M

F

V

G

G

M

I

F

C

P

Y

Q

Q

L

I

G

A

P

F

S

T

I

V

G

G

-

L

-

I

-

L

-

A

-

G

-

V

-

A

F

Y

L

V

A

I

A

P

N

N

G

W

L

R

F

W

L

L

T

Q

L

F

A

A

M

V

S

T

L

L

D

P

D

N

E

F

Q

C

F

F

R

L

D

L

W

Y

G

F

W

W

R

C

I

I

P

P

-

E

-

S

-

P

-

R

F

W

L

L

L

I

S

S

A

Q

V

N

L

K

V

I

I

V

V

K

G

A

L

M

Y

K

V

I

-

I

-

K

-

H

-

I

-

A

R

K

L

K

K

N

L

G

H

K

E

S

T

V

P

P

V

V

F

S

A

L

N

Q

A

N

M

L

A

T

R

P

Q

D

E

E

R

D

V

A

K

G

V

K

P

K

L

L

V

N

E

P

L

S

F

F

S

L

Q

D

Y

L

W

V

A

R

P

T

T

P

L

Q

L

I

G

R

A

K

A

H

A

T

M

L

V

I

V

L

C

M

Y

Y

A

N

L

W

F

F

Y

T

I

S

S

S

T

V

V

L

F

Y

S

Q

L

-

S

-

Y

-

G

G

-

L

V

I

S

M

T

H

L

M

G

G

Y

L

S

A

R

G

E

D

T

N

F

I

L

Y

G

L

L

D

L

F

C

F

F

Y

A

S

V

A

L

L

F

V

M

E

A

F

A

P

A

A

T

A

P

F

L

I

S

I

A

I

W

L

A

T

S

I

D

D

R

R

Y

V

G

G

R

R

K

R

P

Y

I

P

L

W

I

A

I

V

G

S

G

N

L

M

L

V

A

A

-

G

-

A

-

C

I

L

A

A

S

S

G

V

F

F

L

I

M

P

E

D

P

D

L

L

L

Q

T

W

H

L

G

K

S

I

T

T

G

I

G

A

V

C

A

-

L

-

F

-

L

-

C

-

I

-

E

-

L

L

F

G

L

R

M

M

G

G

V

I

T

T

F

M

A

A

-

Y

P

E

M

M

G

V

A
x
C

L

L

L

V

P

N

-

A

E

E

L

L

F

Y

P

P

T

T

H

Y

V

I

R

R

Y

N

T

L

G

G

-

V

-

L

-

V

A

C

S

S

A

S

A

M

Y

C

N

D

L

I

G

G

I

I

V

I

G

-

A

-

S

-

A

-

A

T

P

P

F

F

F

L

A

V

Q

Y

K

R

L

L

V

T

A

D

M

I

C451 (≠ A361) Involved in recognition of organic cations and participates in structural changes that occur during translocation of organic cations; mutation to M: Transport activity strongly reduced.

8sc6A Human oct1 bound to thiamine in inward-open conformation (see paper)
29% identity, 38% coverage: 35:200/437 of query aligns to 105:266/447 of 8sc6A

query
sites
8sc6A

D

D

F

G

Y

W

V

V

Y

Y

A

D

T

T

A

P

A

G

A

S

L

S

V

I

I

V

G

T

P

E

V

F

-

N

F

L

F

V

P

C

Q

A

T

D

S

S

G

W

T

K

A

L

Q

D

M

L

L

F

S

Q

S

S

F

C

L

L

T

N

F

A

G

G

I

F

A

L

F

F

L

-

A

-

R

-

P

-

L

-

G

G

S

S

A

L

L

G

F

V

G

G

H

Y

F

F

G

A

D

D

R

R

I

F

G

G

R

R

K

K

S

L

T

C

L

L

V

L

A

G

S

T

L

V

L

L

L

V

M

N

G

A

V

V

C

S

T

G

T

V

L

L

I

M

G

A

V

F

L

S

P

P

G

N

Y

Y

A

M

S

S

I

-

G

-

A

-

W

-

A

-

P

-

I

-

L

-

L

M

C

L

L

L

L

F

R

R

F

L

G

L

Q

Q

G

G

L

L

G

V

L

S

G

K

G

G

E

N

W

W

G

M

G

A

A

G

A

Y

L

T

L

L

A

I

T

T

E

E

N

F

A

V

P

G

K

S

G

G

K

S

R

R

A

R

W

T

F

V

G

A

M

I

F

M

P

Y

Q
|
Q

L

M

G

A

P
x
F

S

T

I

V

G

G

F

L

L

V

A

A

A

L

N

T

G

G

L

L

-

A

-

Y

-

A

-

L

-

P

-

H

-

W

-

R

F

W

L

L

T

Q

L

L

A

A

M

V

S

S

L

L

D

P

D

T

E

F

Q

L

F

F

R

L

D

L

W

Y

G

Y

W

W

R

S

I

P

P

S

F

F

binding 3-(4-amino-2-methyl-pyrimidin-5-ylmethyl)-5-(2-hydroxy-ethyl)-4-methyl-thiazol-3-ium: Q223 (= Q165), F226 (≠ P168)

Sites not aligning to the query:

binding 3-(4-amino-2-methyl-pyrimidin-5-ylmethyl)-5-(2-hydroxy-ethyl)-4-methyl-thiazol-3-ium: 293, 294, 318, 378, 379

Q63089 Solute carrier family 22 member 1; Organic cation transporter 1; rOCT1 from Rattus norvegicus (Rat) (see 4 papers)
24% identity, 74% coverage: 77:401/437 of query aligns to 162:491/556 of Q63089

query
sites
Q63089

L

L

G

G

S

S

A

L

L

V

F

V

G

G

H

Y

F

I

G

A

D

D

R

R

I

F

G

G

R

R

K

K

S

L

T
x
C

L

L

V

L

A

V

S

T

L

T

L

L

L

V

M

T

G

S

V

V

C

S

T

G

T

V

L

L

I

T

G

A

V

V

L

A

P

P

G

D

Y

Y

A

T

S

S

I

-

G

-

A

-

W

-

A

-

P

-

I

-

L

-

L

M

C

L

L

L

L

F

R

R

F

L

G

L

Q

Q

G

G

L
x
M

G
x
V

L
x
S

G
x
K

G
|
G

E
x
S

W
|
W

G
x
V

G
x
S

A
x
G

A
x
Y

L
x
T

L
|
L

A
x
I

T
|
T

E
|
E

N
x
F

A
x
V

P

G

K

S

G

G

K

Y

R

R

A

R

W

T

F

T

G

A

M

I

F

L

P

Y

Q

Q

L

M

G

A

P

F

S

T

I

V

G

G

F

L

L

V

A

G

A

L

N

A

G

G

L

V

-

A

-

Y

-

A

-

I

-

P

-

D

-

W

-

R

F

W

L

L

T

Q

L

L

A

A

M

V

S

S

L

L

D

P

D

T

E

F

Q

L

F

F

R

L

D

L

W

Y

G

Y

W

W

R

F

I

V

P

P

-

E

-
x
S

-

P

-

R

F

W

L

L

S
|
S

A

Q

V

K

L

R

V
x
T

I

T

V

R

G

A

L

V

Y

R

V

I

R

M

L

E

K

Q

L

I

H

A

E

Q

T

K

-

N

-

G

-

K

-

V

-

P

P

P

V

A

F

D

A

L

N

K

A

M

M

L

A
x
C

R

L

Q

E

E

E

R

D

V

A

K
x
S

V

E

P

K

L

R

V

S

E

P

L

S

F

F

S

A

Q

D

Y

L

W

F

A

R

P

T

T

P

L

N

L

L

G

R

A

K

A

H

A

T

M

V

V

I

V

L

C

M

Y

Y

A

L

L

W

F

F

Y

S

I
x
C

S

A

T

V

V

L

F

Y

S

Q

-

G

L

L

S

I

Y

M

G

H

V

V

S

G

T

A

L

T

G

G

-

A

-

N

-

L

Y

Y

S

L

R

D

E

F

T

F

F

Y

L

S

G

S

L

L

L

V

C

E

F

F

A

P

V

A

L

A

F

F

M

I

A

I

A

L

A

V

T

T

P

-

L

-

S

-

A

-

W

-

A

-

S

I

D

D

R

R

Y

I

G

G

R

R

K

I

P

Y

I

P

L

I

I

A

G

S

G

N

L

L

L

V

A

T

I

G

A

A

S

A

G
x
C

F

L

L

L

M

M

E

-

P

-

L

-

T

-

H

-

G

-

S

-

T

-

G

-

V

-

A

-

L

I

F

F

L

I

C

P

I

H

E

E

L

L

F

H

L

W

M

L

G

N

V

V

T

T

F

L

A

A

P
x
C

M

L

G

G

A

R

L

M

-

G

-

A

-

T

-

I

-

V

-

L

-

Q

-

M

-

V

-
x
C

-

L

-

V

L

N

P

A

E

E

L

L

F

Y

P

P

T

T

H

F

V

I

R

R

Y

N

T

L

G

G

-

M

-

V

A
x
C

S

S

A

A

A

L

Y
x
C

N
x
D

L

L

G

G

I

I

V

F

G

-

A

-

S

-

A

-

A

T

P

P

F

F

F

M

A

V

Q

F

K

R

L

L

V

M

A

E

M

V

C179 (≠ T94) mutation to A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; S-322; A-358; A-418; S-437; A-470 and A-474.
M212 (≠ L135) mutation to L: No change in TEA and MPP(+) uptake.
V213 (≠ G136) mutation to G: Decreased TEA uptake. No change in MPP(+) uptake.
S214 (≠ L137) mutation to G: Decreased TEA and MPP(+) uptake.
K215 (≠ G138) mutation to Q: Loss of TEA and MPP(+) uptake activity.; mutation to R: Loss of TEA and MPP(+) uptake activity.
G216 (= G139) mutation to A: Decreased TEA and MPP(+) uptake.
S217 (≠ E140) mutation to G: No change in TEA and MPP(+) uptake.
W218 (= W141) mutation to F: Decreased guanidine, histamine, serotonin and TEA uptake. No change in MPP(+) uptake. No change in TEA and MPP(+) affinity. Decreased TEA Vmax. No change in MPP(+) Vmax.; mutation to L: Decreased guanidine, histamine, serotonin, TEA and MPP(+) uptake. Decreased TEA affinity. No change in MPP(+) affinity. Decreased TEA and MPP(+) Vmax.; mutation to Y: Decreased guanidine, histamine, serotonin and TEA uptake. No change in MPP(+) uptake. Increased TEA and MPP(+) affinity. Decreased TEA and MPP(+) Vmax.
V219 (≠ G142) mutation to L: No change in TEA and MPP(+) uptake.
S220 (≠ G143) mutation to I: Decreased TEA and MPP(+) uptake.
G221 (≠ A144) mutation to A: Decreased TEA and MPP(+) uptake.
Y222 (≠ A145) mutation to F: No change in guanidine, histamine, serotonin, TEA and MPP(+) uptake. Increased TEA affinity. No change in MPP(+) affinity. Decreased TEA Vmax. No change in MPP(+) Vmax.; mutation to L: Decreased guanidine, serotonin, TEA and MPP(+) uptake. No change in histamine uptake. Increased TEA and MPP(+) affinity. Decreased TEA and MPP(+) Vmax.
T223 (≠ L146) mutation to I: Decreased TEA uptake. No change in MPP(+) uptake.
L224 (= L147) mutation to V: Decreased TEA and MPP(+) uptake.
I225 (≠ A148) mutation to G: No change in TEA and MPP(+) uptake.
T226 (= T149) mutation to A: Decreased TEA uptake. No change in MPP(+) uptake.
E227 (= E150) mutation to D: Loss of TEA and MPP(+) uptake activity.; mutation to Q: Loss of TEA and MPP(+) uptake activity.
F228 (≠ N151) mutation to I: No change in TEA and MPP(+) uptake.
V229 (≠ A152) mutation to A: Decreased TEA and MPP(+) uptake.; mutation to L: Loss of TEA and MPP(+) uptake activity.
S286 (vs. gap) mutation to A: No effect of PKC-induced stimulation on ASP uptake. No effect of PKC-induced stimulation on ASP uptake; when associated with A-292; A-296; A-328 and A-550. No effect of PKA activation on ASP uptake. No effect of PKA activation on ASP uptake; when associated with A-292; A-296; A-328 and A-550. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition; when associated with A-292; A-296; A-328 and A-550. No significant effect on trafficking from intracellular pools to the cell membrane; when associated with A-292; A-296; A-328 and A-550. suppresses phosphorylation by PKC; when associated with A-292; A-296; A-328 and A-550.
S292 (= S203) mutation to A: No effect of PKC-induced stimulation on ASP uptake. No effect of PKC-induced stimulation on ASP uptake; when associated with A-286; A-296; A-328 and A-550. No effect of PKA activation on ASP uptake. No effect of PKA activation on ASP uptake; when associated with A-286; A-296; A-328 and A-550. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition; when associated with A-286; A-296; A-328 and A-550. No significant effect on trafficking from intracellular pools to the cell membrane; when associated with A-286; A-296; A-328 and A-550. suppresses phosphorylation by PKC; when associated with A-286; A-296; A-328 and A-550.
T296 (≠ V207) mutation to A: No effect of PKC-induced stimulation on ASP uptake. No effect of PKC-induced stimulation on ASP uptake; when associated with A-286; A-292; A-328; A-550. Significant increase of the ASP uptake by PKA activation. No effect of PKA activation on ASP uptake; when associated with A-286; A-292; A-328; A-550. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition; when associated with A-286; A-292; A-328; A-550. No significant effect on trafficking from intracellular pools to the cell membrane; when associated with A-286; A-292; A-328 and A-550. suppresses phosphorylation by PKC; when associated with A-286; A-292; A-328 and A-550.
C322 (≠ A228) mutation to S: Reduces the activation by MMTS. Abolishes the activation by MMTs; when associated with M-451. Choline affinity is increased fivefold by MMTS. Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; A-358; A-418; S-437; A-470 and A-474. Choline affinity is increased four- to fivefold; when associated with M-451.
S328 (≠ K234) mutation to A: No effect of PKC-induced stimulation on ASP uptake. No effect of PKC-induced stimulation on ASP uptake; when associated with A-286; A-292; A-296 and A-550. No effect of PKA activation on ASP uptake. No effect of PKA activation on ASP uptake; when associated with A-286; A-292; A-296 and A-550. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition; when associated with A-286; A-292; A-296; A-550. No significant effect on trafficking from intracellular pools to the cell membrane; when associated with A-286; A-292; A-296 and A-550. suppresses phosphorylation by PKC; when associated with A-286; A-292; A-296 and A-550.
C358 (≠ I264) mutation to A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; S-322; A-418; S-437; A-470 and A-474.
C418 (≠ G326) mutation to A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; S-322; A-358; S-437; A-470 and A-474.
C437 (≠ P358) mutation to S: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; S-322; A-358; A-418; A-470 and A-474.
C451 (vs. gap) mutation to M: Reduces the activation by MMTS. Abolishes the activation by MMTs; when associated with S-322. Abolishes the effect of MMTs on choline-induced currents. Choline affinity is not influenced by MMTS. Choline affinity is increased four- to fivefold; when associated with S-322.
C470 (≠ A376) mutation to A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; S-322; A-358; A-418; A-437 and A-474.
C474 (≠ Y380) mutation to A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; S-322; A-358; A-418; A-437 and A-470.
D475 (≠ N381) mutation to E: Decreased MPP(+) uptake, no change in MPP(+) affinity. Decreased NMN uptake, increased NMN affinity. Decreased choline uptake, increased choline affinity.; mutation to N: Decreased MPP(+) uptake.; mutation to R: Decreased MPP(+) uptake.

Sites not aligning to the query:

26 C→A: Choline affinity is increased fourfold by MMTS; when associated with A-155; A-179; S-322; A-358; A-418; S-437; A-470 and A-474.
155 C→A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-179; S-322; A-358; A-418; S-437; A-470 and A-474.
550 T→A: No effect of PKC-induced stimulation on ASP uptake. No effect of PKC-induced stimulation on ASP uptake; when associated with A-286; A-292; A-296; A-328. Significant increase of the ASP uptake by PKA activation. No effect of PKA activation on ASP uptake; when associated with A-286; A-292; A-296 and A-328. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition; when associated with A-286; A-292; A-296; A-328. No significant effect on trafficking from intracellular pools to the cell membrane; when associated with A-286; A-292; A-296 and A-328. suppresses phosphorylation by PKC; when associated with A-286; A-292; A-296 and A-328.

P36035 Carboxylic acid transporter protein homolog from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
25% identity, 37% coverage: 69:231/437 of query aligns to 182:330/616 of P36035

query
sites
P36035

G

G

I

L

A

V

F

L

L

F

A

V

R

R

P

S

L

A

G

G

S

A

A

V

L

I

F

F

G

G

H

L

F

W

G

T

D

D

R

K

I

S

G

S

R

R

K

K

S

W

T

P

L

Y

V

I

A

T

S

C

L

L

L

F

L

L

M

F

G

V

V

I

-

A

-

Q

-

L

C

C

T

T

T

P

L

W

I

C

G

D

V

T

L

Y

P

E

G

K

Y

F

A

L

S

G

I

V

G

-

A

-

W

-

A

-

P

-

I

-

L

-

C

-

L

-

R

R

F

W

G

I

Q

T

G

G

L

I

G

A

L

M

G

G

E

I

W

Y

G

G

G

C

A

A

A

S

L

A

L

T

A

A

T

I

E

E

N

D

A

A

P

P

K

V

G

K

K

A

R

R

A

S

W

F

F

L

G

S

M

G

F

L

P

F

Q

F

L

S

G

A

P

Y

S

A

I

M

G

G

F

F

L

I

A

F

A

A

N

I

G

I

L

F

F

Y

L

R

T

A

L

F

A

G

M

Y

S

-

L

-

D

-

E

-

Q

-

F

F

R

R

D

D

W

D

G

G

W

W

R

K

I

I

P

L

F

F

L

W

L

F

S

S

A

I

V

F

L

L

V

P

I

I

V

L

G

L

L

I

Y

F

V

W

R

R

L

L

K

L

L

W

H

P

E

E

T

T

P

K

V

Y

F

F

A

T

N

K

A

V

M

L

A

K

R

A

Q

R

E

K

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
9 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
338 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

8sc3A Human oct1 bound to fenoterol in inward-open conformation (see paper)
29% identity, 33% coverage: 35:178/437 of query aligns to 105:236/445 of 8sc3A

query
sites
8sc3A

D

D

F

G

Y

W

V

V

Y

Y

A

D

T

T

A

P

A

G

A

S

L

S

V

I

I

V

G

T

P

E

V

F

-

N

F

L

F

V

P

C

Q

A

T

D

S

S

G

W

T

K

A

L

Q

D

M

L

L

F

S

Q

S

S

F

C

L

L

T

N

F

A

G

G

I

F

A

L

F

F

L

-

A

-

R

-

P

-

L

-

G

G

S

S

A

L

L

G

F

V

G

G

H

Y

F

F

G

A

D

D

R

R

I

F

G

G

R

R

K

K

S

L

T

C

L

L

V

L

A

G

S

T

L

V

L

L

L

V

M

N

G

A

V

V

C

S

T

G

T

V

L

L

I

M

G

A

V

F

L

S

P

P

G

N

Y

Y

A

M

S

S

I

-

G

-

A

-

W

-

A

-

P

-

I

-

L

-

L

M

C

L

L

L

L

F

R

R

F

L

G

L

Q

Q

G

G

L

L

G

V

L

S

G

K

G

G

E

N

W
|
W

G
x
M

G

A

A

G

A

Y

L

T

L

L

A

I

T

T

E

E

N

F

A

V

P

G

K

S

G

G

K

S

R

R

A

R

W

T

F

V

G

A

M

I

F

M

P

Y

Q
|
Q

L

M

G

A

P
x
F

S

T

I

V

G

G

F

L

L

V

A

A

A

L

N

T

G

G

L

L

binding Fenoterol: W199 (= W141), M200 (≠ G142), Q223 (= Q165), F226 (≠ P168)

Sites not aligning to the query:

binding Fenoterol: 284, 291, 376, 380

Q9LT15 Sugar transport protein 10; AtSTP10; D-glucose-H(+) symport protein STP10; D-glucose-proton symporter STP10; Hexose transporter 10 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
21% identity, 70% coverage: 125:429/437 of query aligns to 137:478/514 of Q9LT15

query
sites
Q9LT15

L

M

L

L

C

I

L

I

L

G

R

R

F

L

G

L

Q

L

G

G

L

V

G

G

L

V

G

G

G

F

E

A

W

N

G

Q

G

S

A

T

A

P

L

V

L

Y

A

L

T

S

E
|
E

N

M

A

A

P

P

K

A

G

K

K

I

R

R

A

G

W

A

F

L

G

N

M

I

F

G

P

F

Q
|
Q

L

M

G

A

P

I

S

T

I

I

G

G

F
x
I

L

L

A

V

A

A

N

N

G

-

L

-

F

-

L

-

T

-

L

-

A

L

M

I

S

N

L

Y

D

G

D

T

E

S

Q

K

F

M

R

A

D

Q

W

H

G

G

W

W

R

R

I

V

P

S

F

L

L

G

L

L

S

A

A

A

V

V

-

P

-

A

-

V

L

V

V

M

I

V

V

I

G

G

L

S

Y

F

V

I

-

L

-

P

-

D

-

T

-

P

-

N

-

S

-

M

-

L

-

E

-

R

-

G

-

K

-

N

-

E

-

A

-

K

-

Q

R

M

L

L

K

K

L

K

-

I

-

R

-

G

-

A

-

D

-

N

-

V

-

D

H

H

E

E

T

F

P

Q

V

D

F

L

A

I

N

D

A

A

M

V

A

E

R

A

Q

A

E

K

R

K

V

V

K

E

V

N

P

P

L

W

V

K

E

N

L

I

F

M

S

E

Q

S

Y

K

W

Y

A

R

P

P

T

A

L

L

L

I

G

F

A

C

A

S

A

A

M

I

V

P

-

F

-
x
Q

-

I

-

T

-

G

V

I

C
x
N

Y

V

A

I

L

M

F

F

Y

Y

I

A

S

P

T

V

V

L

F

F

S

K

L

-

S

-

Y

-

G

-

V

-

S

-

T

T

L

L

G

G

Y

F

S

G

R

D

E

D

T

A

F

A

L

L

G

M

L

S

L

A

C

V

F

I

A

T

V

G

L

V

F

V

M
x
N

A

M

A

L

A

S

T

T

P

F

L

V

S

S

A

I

W

Y

A

A

S

V

D
|
D

R

R

Y

Y

G

G

R

R

K

R

P

L

I

L

L

F

I

L

I

E

G

G

L

I

L

Q

A

M

I

F

A

I

S

C

G

Q

F

L

L

L

M

V

E

G

P

S

L

F

-

I

-

G

-

A

-

R

-

F

-

G

-

T

-

S

-

G

-

T

-

G

-

T

L

L

T

T

H

P

G

A

S

T

T

A

G

D

G

W

V

I

A

L

L

A

F

F

L

I

C

C

I

V

E

Y

L

V

F

A

L

G

M

F

G

A

V

W

T

S

F

W

A

G

P

P

M

L

G

G

A
x
W

L

L

L

V

P

P

-

S

E

E

L

I

F

C

P

P

T

L

H

E

V

I

R

R

Y

P

T

A

G

G

A

Q

S

A

A

I

A

N

Y

V

N

S

L

V

G

N

G

M

I

F

V

F

G

T

A

F

S

L

A

I

A

G

P

Q

F

F

A

L

Q

T

K

M

L

L

V
x
C

A

H

M

M

G

K

-

F

G

G

L

L

S

F

Y

Y

V

F

G

-

Y

F

V

A

S

S

A

M

A

V

A

A

L

I

L

M

S

T

V

V

I

F

A

I

V

Y

L

F

C

L

L

L

K

P

E

E

T

T

R

K

E162 (= E150) mutation to Q: Abolishes glucose transport activity; when associated with N-344.
Q177 (= Q165) binding ; mutation to A: Reduces affinity for glucose 37-fold.
I184 (≠ F172) mutation to A: Reduces affinity for glucose 3-fold.
Q295 (vs. gap) binding
Q296 (vs. gap) binding
N301 (≠ C258) binding
N332 (≠ M295) binding
D344 (= D307) mutation to N: Abolishes glucose transport activity; when associated with Q-162.
W410 (≠ A361) binding
C449 (≠ V399) modified: Disulfide link with 77; mutation to A: Increases sensitivity to alkaline pH and can only function fully at acidic pH (pH < 5).

Sites not aligning to the query:

39 F→A: Reduces affinity for glucose 8-fold.
43 L→A: Reduces affinity for glucose 150-fold and turns STP10 into a low affinity transporter.
77 modified: Disulfide link with 449; C→A: Increases sensitivity to alkaline pH and can only function fully at acidic pH (pH < 5).

7aaqA Sugar/h+ symporter stp10 in outward occluded conformation (see paper)
21% identity, 70% coverage: 125:429/437 of query aligns to 117:458/487 of 7aaqA

query
sites
7aaqA

L

M

L

L

C

I

L

I

L

G

R

R

F

L

G

L

Q

L

G

G

L

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G

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L

V

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G

G

F

E

A

W

N

G

Q

G

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A

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A

P

L

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Y

A

L

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E

E

N

M

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A

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A

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A

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Q
|
Q

L

M

G

A

P

I

S

T

I

I

G

G

F
x
I

L

L

A

V

A

A

N

N

G

-

L

-

F

-

L

-

T

-

L

-

A

L

M

I

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N

L

Y

D

G

D

T

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Q

K

F

M

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A

D

Q

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H

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G

W

W

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R

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F

L

L

G

L

L

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A

A

A

V

V

-

P

-

A

-

V

L

V

V

M

I

V

V

I

G

G

L

S

Y

F

V

I

-

L

-

P

-

D

-

T

-

P

-

N

-

S

-

M

-

L

-

E

-

R

-

G

-

K

-

N

-

E

-

A

-

K

-

Q

R

M

L

L

K

K

L

K

-

I

-

R

-

G

-

A

-

D

-

N

-

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-

D

H

H

E

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D

A

A

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A

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A

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Q

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F

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Y

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D

E

D

T

A

F

A

L

L

G

M

L

S

L

A

C

V

F

I

A

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G

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V

F

V

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x
N

A

M

A

L

A

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T

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F

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S

A

I

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Y

A

A

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D

D

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Y

Y

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G

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L

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I

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A

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I

F

A

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F

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G

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A

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A

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A
x
G

P

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A
x
W

L

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-

S

E

E

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L

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E

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A

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F

A

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L

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C

A

H

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M

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-

F

G

G

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binding beta-D-glucopyranose: Q157 (= Q165), I164 (≠ F172), Q275 (vs. gap), N281 (≠ C258), N312 (≠ M295), G386 (≠ A357), W390 (≠ A361)

Q9Y7Q9 Probable metabolite transporter C2H8.02 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
25% identity, 34% coverage: 77:226/437 of query aligns to 98:254/583 of Q9Y7Q9

query
sites
Q9Y7Q9

L

M

G

G

S

Q

A

L

L

L

F

F

G

G

H

F

F

L

G

G

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D

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A

A

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A

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A

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E

E

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Q

A

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G

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L

A

A

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W

F

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Q

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L

W

G

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A

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L

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I

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L

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D

D

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-

G

-

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W

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A

A

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N

N

A

S

Sites not aligning to the query:

267 modified: Phosphoserine
269 modified: Phosphoserine
289 modified: Phosphoserine
290 modified: Phosphoserine
292 modified: Phosphoserine
330 modified: Phosphoserine

Query Sequence

>AO356_06425 FitnessBrowser__pseudo5_N2C3_1:AO356_06425
MTTATCNEAVPAQPTNSATRVATASFIGTAIEFYDFYVYATAAALVIGPVFFPQTSGTAQ
MLSSFLTFGIAFLARPLGSALFGHFGDRIGRKSTLVASLLLMGVCTTLIGVLPGYASIGA
WAPILLCLLRFGQGLGLGGEWGGAALLATENAPKGKRAWFGMFPQLGPSIGFLAANGLFL
TLAMSLDDEQFRDWGWRIPFLLSAVLVIVGLYVRLKLHETPVFANAMARQERVKVPLVEL
FSQYWAPTLLGAAAMVVCYALFYISTVFSLSYGVSTLGYSRETFLGLLCFAVLFMAAATP
LSAWASDRYGRKPILIIGGLLAIASGFLMEPLLTHGSTGGVALFLCIELFLMGVTFAPMG
ALLPELFPTHVRYTGASAAYNLGGIVGASAAPFFAQKLVAMGGLSYVGGYVSAAALLSVI
AVLCLKETRHNDLNRVA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory