SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO356_06655 FitnessBrowser__pseudo5_N2C3_1:AO356_06655 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
46% identity, 65% coverage: 8:175/258 of query aligns to 84:251/262 of 1t3dA

query
sites
1t3dA

D

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binding cysteine: D92 (= D16), P93 (= P17), D157 (= D81), G184 (= G108), R192 (= R116), H193 (= H117)

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
46% identity, 65% coverage: 8:175/258 of query aligns to 80:247/258 of 8i04A

query
sites
8i04A

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binding serine: D88 (= D16), P89 (= P17), D153 (= D81), H154 (= H82), G180 (= G108), R188 (= R116), H189 (= H117)

8i09A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with butyl gallate (see paper)
47% identity, 63% coverage: 8:170/258 of query aligns to 83:245/246 of 8i09A

query
sites
8i09A

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binding cysteine: D91 (= D16), D156 (= D81), H157 (= H82), G164 (= G89), R191 (= R116), H192 (= H117)
binding butyl 3,4,5-tris(oxidanyl)benzoate: G183 (= G108), T184 (= T109), G202 (= G127), K218 (= K143), I219 (≠ V144), G220 (= G145), L226 (≠ T151), A236 (≠ V161)

8i06A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with coa (see paper)
47% identity, 62% coverage: 8:168/258 of query aligns to 84:244/244 of 8i06A

query
sites
8i06A

D

D

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I

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Q

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A

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P

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A

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D

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binding coenzyme a: H158 (= H82), L177 (≠ Y101), Q178 (= Q102), G203 (= G127), A204 (= A128), G221 (= G145), A222 (≠ S146), T235 (= T159), A237 (≠ V161)
binding cysteine: P93 (= P17), H158 (= H82)

1ssqD Serine acetyltransferase- complex with cysteine (see paper)
47% identity, 62% coverage: 8:168/258 of query aligns to 80:240/257 of 1ssqD

query
sites
1ssqD

D

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binding cysteine: D88 (= D16), D153 (= D81), H154 (= H82)

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
47% identity, 62% coverage: 8:168/258 of query aligns to 80:240/258 of 4h7oA

query
sites
4h7oA

D

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binding arginine: Y127 (≠ L55), Q131 (≠ F59)
binding cysteine: D88 (= D16), P89 (= P17), D153 (= D81), H154 (= H82), G180 (= G108), R188 (= R116), H189 (= H117)

Sites not aligning to the query:

binding arginine: 51, 53

4hzdA Crystal structure of serine acetyltransferase in complex with coenzyme a from brucella abortus strain s19 (see paper)
44% identity, 64% coverage: 5:168/258 of query aligns to 81:244/250 of 4hzdA

query
sites
4hzdA

L

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I

binding coenzyme a: H158 (= H82), G203 (= G127), A204 (= A128), A221 (≠ G145), A222 (≠ S146), T235 (= T159), A237 (≠ V161), I244 (= I168)
binding magnesium ion: S224 (≠ A148), A237 (≠ V161), G238 (= G162)

7bw9A Crystal structure of serine acetyltransferase isoform 3 in complex with cysteine from entamoeba histolytica
45% identity, 59% coverage: 3:154/258 of query aligns to 101:252/280 of 7bw9A

query
sites
7bw9A

E

E

R

L

L

V

R

K

E

Q

D

D

I

I

Q

I

S

A

V

A

F

Y

H

T

R

G

D
|
D

P
|
P

A

A

A

A

R

S

N

S

A

L

F

A

E

M

V

I

L

I

T

R

C

S

Y

Y

P

P

G

G

M

I

H

H

A

V

I

M

W

M

I

I

H

Q

R

R

L

V

S

A

G

H

A

I

L

L

W

Y

N

M

M

N

G

G

W

D

K

I

W

E

L

Y

A

S

R

R

L

E

V

L

S

M

N

E

F

N

G

I

R

H

W

S

L

V

T

T

G

G

I

I

E

D

I

I

H

H

P

P

G

G

A

T

K

S

V

I

G

G

R

N

R

H

F

F

F

F

I

I

D
|
D

H
|
H

G

G

M

V

G

G

I

V

V

V

I

I

G

G

E

E

T

T

A

A

E

V

I

I

G

G

D

N

D

W

V

C

T

R

L

V

Y

Y

Q

Q

G

S

V

V

T

T

L

L

G

G

G
x
A

T

M

S

S

W

F

N

K

K

G

G

N

K

K

R
|
R

H
|
H

P

P

T

T

L

I

E

G

D

D

G

F

V

V

V

V

V

I

G

G

A

A

G

G

A

A

K

K

V

V

L

L

G

G

P

N

F

I

T

T

V

I

G

G

A

S

G

N

A

V

K

K

V

I

G

G

S

A

N

N

A

C

V

W

V

I

T

T

K

Q

A

N

V

I

binding cysteine: D114 (= D16), P115 (= P17), D179 (= D81), H180 (= H82), A206 (≠ G108), R214 (= R116), H215 (= H117)

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
40% identity, 64% coverage: 8:171/258 of query aligns to 85:248/261 of 6wyeA

query
sites
6wyeA

D

D

I

L

Q

K

S

A

V

I

F

Y

H

E

R

R

D
|
D

P
|
P

A

A

A

C

R

D

N

E

A

Y

F

S

E

L

V

P

L

L

T

L

C

Y

Y

F

P

K

G

G

M

F

H

H

A

A

I

I

W

Q

I

A

H

H

R

R

L

I

S

N

G

H

A

R

L

L

W

Y

N

L

M

D

G

G

W

R

K

K

W

T

L

L

A

A

R

Y

L

F

V

L

S

Q

N

N

F

R

G

M

R

S

W

E

L

V

T

F

G

G

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

R

V

L

G

G

R

Y

R

G

F

L

F

M

I

L

D
|
D

H
|
H

G

A

M

T

G

G

I

F

V

V

I

A

G

G

E

E

T

T

A

A

E

V

I

L

G

G

D

N

D

N

V

I

T

S

L

I

Y

L

Q

H

G

G

V

V

T

T

L

L

G

G

G
|
G

T

S

S

G

W

K

N

E

K

G

G

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

E

G

D

D

G

G

V

V

V

M

V

I

G

G

A

A

G

N

A

A

K

S

V

I

L

L

G

G

P

N

F

I

T

R

V

I

G

G

A

S

G

N

A

A

K

K

V

I

G

G

S

A

N

G

A

S

V

V

V

V

T

V

K

S

A

D

V

V

P

P

P

P

G

S

A

I

T

T

V

V

V

V

G

G

I

V

P

P

G

A

R

K

I

P

I

V

V

A

K

R

S

S

binding (2S)-2-hydroxybutanedioic acid: D93 (= D16), P94 (= P17), D158 (= D81), H159 (= H82), G185 (= G108), R193 (= R116), H194 (= H117)

1sstA Serine acetyltransferase- complex with coa (see paper)
46% identity, 62% coverage: 8:168/258 of query aligns to 80:233/233 of 1sstA

query
sites
1sstA

D

D

I

I

Q

Q

S

A

V

V

F

R

H

H

R

R

D

D

P

P

A

A

A

V

R

E

N

L

A

W

F

S

E

T

V

P

L

L

T

L

C

Y

Y

L

P

K

G

G

M

F

H

H

A

A

I

I

W

Q

I

S

H

Y

R

R

L

I

S

T

G

H

A

Y

L

L

W

W

N

N

M

Q

G

N

W

R

K

K

W

S

L

L

A

A

R

L

L

Y

V

L

S

Q

N

N

F

Q

G

I

R

S

W

V

L

A

T

F

G

D

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

K

V

I

G

G

R

H

R

G

F

I

F

M

I

F

D

D

H
|
H

G

A

M

T

G

G

I

I

V

V

I

V

G

G

E

E

T

T

A

S

E

V

I

I

G

E

D

N

D

D

V

V

T

S

L

I

Y
x
L

Q

Q

G

G

V

V

T

T

L

L

G

G

G
|
G

T
|
T

S

-

W

-

N

-

K

-

G

-

K

-

R

-

H

H

P

P

T

K

L

V

E

R

D

E

G

G

V

V

V

M

V

I

G

G

A
|
A

G

G

A

A

K

K

V

I

L
|
L

G

G

P

N

F

I

T

E

V

V

G

G

A

K

G

Y

A

A

K
|
K

V

I

G
|
G

S
x
A

N

N

A

S

V
|
V

V

V

T
x
L

K

N

A

P

V

V

P

P

P

E

G

Y

A

A

T
|
T

V

A

V
x
A

G

G

I
x
V

P
|
P

G

A

R

R

I

I

I
x
V

binding coenzyme a: H154 (= H82), L173 (≠ Y101), G180 (= G108), T181 (= T109), A193 (= A128), L198 (= L133), K208 (= K143), G210 (= G145), A211 (≠ S146), V214 (= V149), L216 (≠ T151), T224 (= T159), A226 (≠ V161), V228 (≠ I163), P229 (= P164), V233 (≠ I168)

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
41% identity, 62% coverage: 8:166/258 of query aligns to 83:241/243 of 7ra4A

query
sites
7ra4A

D

D

I

L

Q

K

S

A

V

I

F

Y

H

E

R

R

D
|
D

P
|
P

A

A

A

C

R

D

N

E

A

Y

F

S

E

L

V

P

L

L

T

L

C

Y

Y

F

P

K

G

G

M

F

H

H

A

A

I

I

W

Q

I

A

H

H

R

R

L

I

S

N

G

H

A

R

L

L

W

Y

N

L

M

D

G

G

W

R

K

K

W

T

L

L

A

A

R

Y

L

F

V

L

S

Q

N

N

F

R

G

M

R

S

W

E

L

V

T

F

G

G

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

R

V

L

G

G

R

Y

R

G

F

L

F

M

I

L

D
|
D

H
|
H

G

A

M

T

G

G

I

F

V

V

I

A

G

G

E

E

T

T

A

A

E

V

I

L

G

G

D

N

D

N

V

I

T

S

L

I

Y

L

Q

H

G

G

V

V

T

T

L

L

G

G

G

G

T

S

S

G

W

K

N

E

K

G

G

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

E

G

D

D

G

G

V

V

V

M

V

I

G

G

A

A

G

N

A

A

K

S

V

I

L

L

G

G

P

N

F

I

T

R

V

I

G

G

A

S

G

N

A

A

K

K

V

I

G

G

S

A

N

G

A

S

V

V

V

V

T

V

K

S

A

D

V

V

P

P

P

P

G

S

A

I

T

T

V

V

V

V

G

G

I

V

P

P

G

A

R

K

binding serine: D91 (= D16), P92 (= P17), D156 (= D81), H157 (= H82), R191 (= R116), H192 (= H117)

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
42% identity, 68% coverage: 1:175/258 of query aligns to 80:254/272 of 3gvdI

query
sites
3gvdI

M

M

F

I

E

V

R

S

L

A

R

A

E

R

D

D

I

I

Q

L

S

A

V

V

F

R

H

L

R

R

D
|
D

P
|
P

A

A

A

V

R

D

N

K

A

Y

F

S

E

T

V

P

L

L

T

L

C

Y

Y

L

P

K

G

G

M

F

H

H

A

A

I

L

W

Q

I

A

H

Y

R

R

L

I

S

G

G

H

A

W

L

L

W

W

N

A

M

Q

G

D

W

R

K

K

W

A

L

L

A

A

R

I

L

Y

V

L

S

Q

N

N

F

Q

G

V

R

S

W

V

L

A

T

F

G

G

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

T

V

I

G

G

R

C

R

G

F

I

F

M

I

L

D
|
D

H

H

G

A

M

T

G

G

I

I

V

V

I

I

G

G

E

E

T

T

A

A

E

V

I

V

G

E

D

N

D

D

V

V

T

S

L

I

Y

L

Q

Q

G

S

V

V

T

T

L

L

G
|
G

G
|
G

T

T

S

G

W

K

N

T

K

S

G

G

K

D

R
|
R

H

H

P

P

T

K

L

I

E

R

D

E

G

G

V

V

V

M

V

I

G

G

A

A

G

G

A

A

K

K

V

I

L

L

G

G

P

N

F

I

T

E

V

V

G

G

A

R

G

G

A

A

K

K

V

I

G

G

S

A

N

G

A

S

V

V

V

V

T

L

K

Q

A

S

V

V

P

P

P

A

G

H

A

T

T

T

V

A

V

A

G

G

I

V

P

P

G

A

R

R

I

I

I

V

V

G

K

K

S

P

D

E

E

S

E

D

Q

K

binding cysteine: D95 (= D16), P96 (= P17), D160 (= D81), G186 (= G107), G187 (= G108), R195 (= R116)

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
41% identity, 62% coverage: 8:168/258 of query aligns to 83:243/243 of 4n69A

query
sites
4n69A

D

D

I

L

Q

R

S

A

V

A

F

R

H

E

R

R

D

D

P

P

A

A

A

C

R

V

N

S

A

Y

F

S

E

H

V

C

L

L

T

L

C

N

Y

Y

P

K

G

G

M

F

H

L

A

A

I

C

W

Q

I

A

H

H

R

R

L

V

S

A

G

H

A

L

L

L

W

W

N

R

M

Q

G

S

W

R

K

R

W

P

L

L

A

A

R

L

L

A

V

L

S

H

N

S

F

R

G

I

R

A

W

N

L

V

T

F

G

A

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

R

V

I

G

G

R

K

R

G

F

I

F

L

I

F

D

D

H

H

G

A

M

T

G

G

I

V

V

V

I

V

G

G

E

E

T

T

A

A

E

V

I

I

G

G

D

N

D

N

V

V

T

S

L

I

Y

L

Q

H

G

H

V

V

T

T

L

L

G

G

G
|
G

T

T

S

G

W

K

N

V

K

G

G

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

E

G

D

D

G

G

V

V

V

L

V

I

G

G

A

A

G

G

A

A

K

T

V

I

L

L

G

G

P

N

F

I

T

K

V

I

G

G

A

E

G

G

A

A

K

K

V

V

G

G

S

A

N

G

A

S

V

V

V

V

T

L

K

I

A

D

V

V

P

P

P

P

G

R

A

T

T

T

V

A

V

V

G

G

I

N

P

P

G

A

R

R

I

L

I

V

binding serine: G183 (= G108), R191 (= R116), H192 (= H117)

3p47A Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-cysteine (see paper)
39% identity, 60% coverage: 5:160/258 of query aligns to 105:268/270 of 3p47A

query
sites
3p47A

L

L

R

K

E

T

D

D

I

L

Q

I

S

A

V

A

F

Y

H

A

R

G

D

D

P

P

A

A

A

A

R

P

N

G

A

L

F

S

E

L

V

I

L

I

T

R

C

C

Y

Y

P

P

G

G

M

F

H

Q

A

A

I

V

W

I

I

V

H

Y

R

R

L

I

S

A

G

H

A

V

L

L

W

Y

N

E

M

C

G

G

W

E

K

R

W

Y

L

Y

A

C

R

R

L

E

V

M

S

M

N

E

F

S

G

V

R

H

W

S

L

Y

T

T

G

S

I

I

E

D

I

I

H

H

P

P

G

G

A

A

K

S

V

I

G

K

R

G

R

H

F

F

F

F

I

I

D

D

H

H

G

G

M

V

G

G

I

V

V

V

I

I

G

G

E

E

T

T

A

A

E

I

I

I

G

G

D

E

D

W

V

C

T

R

L

I

Y

Y

Q

Q

G

S

V

V

T

T

L

L

G

G

G
x
A

T

M

S
x
H

W

F

N

Q

K

E

-

E

-

G

-

G

-

V

-

I

-

K

-

R

-

G

G

T

K

K

R
|
R

H
|
H

P

P

T

T

L

V

E

G

D

D

G

Y

V

V

V

T

V

I

G

G

A

T

G

G

A

A

K

K

V

V

L

L

G

G

P

N

F

I

T

I

V

V

G

G

A

S

G

H

A

V

K

R

V

I

G

G

S

A

N

N

A

C

V

W

V

I

T

D

K

R

A

D

V

V

P

D

P

S

G

N

A

Q

T

T

V

V

binding cysteine: A208 (≠ G108), H210 (≠ S110), R224 (= R116), H225 (= H117)

3q1xA Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-serine (see paper)
39% identity, 60% coverage: 5:160/258 of query aligns to 103:266/267 of 3q1xA

query
sites
3q1xA

L

L

R

K

E

T

D

D

I

L

Q

I

S

A

V

A

F

Y

H

A

R

G

D

D

P

P

A

A

A

A

R

P

N

G

A

L

F

S

E

L

V

I

L

I

T

R

C

C

Y

Y

P

P

G

G

M

F

H

Q

A

A

I

V

W

I

I

V

H

Y

R

R

L

I

S

A

G

H

A

V

L

L

W

Y

N

E

M

C

G

G

W

E

K

R

W

Y

L

Y

A

C

R

R

L

E

V

M

S

M

N

E

F

S

G

V

R

H

W

S

L

Y

T

T

G

S

I

I

E

D

I

I

H

H

P

P

G

G

A

A

K

S

V

I

G

K

R

G

R

H

F

F

F

F

I

I

D

D

H

H

G

G

M

V

G

G

I

V

V

V

I

I

G

G

E

E

T

T

A

A

E

I

I

I

G

G

D

E

D

W

V

C

T

R

L

I

Y

Y

Q

Q

G

S

V

V

T

T

L

L

G

G

G

A

T

M

S
x
H

W

F

N

Q

K

E

-

E

-

G

-

G

-

V

-

I

-

K

-

R

-

G

G

T

K

K

R
|
R

H
|
H

P

P

T

T

L

V

E

G

D

D

G

Y

V

V

V

T

V

I

G

G

A

T

G

G

A

A

K

K

V

V

L

L

G

G

P

N

F

I

T

I

V

V

G

G

A

S

G

H

A

V

K

R

V

I

G

G

S

A

N

N

A

C

V

W

V

I

T

D

K

R

A

D

V

V

P

D

P

S

G

N

A

Q

T

T

V

V

binding serine: H208 (≠ S110), R222 (= R116), H223 (= H117)

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
37% identity, 64% coverage: 5:168/258 of query aligns to 58:233/233 of 4n6bA

query
sites
4n6bA

L

L

R

Y

E

D

D

L

I

F

Q

L

S

N

V

A

F

F

H

S

R

S

D

D

P

P

A

S

A

L

R

R

N

S

A

A

-

A

-

V

-

A

-

D

-

L

-

R

-

A

-

A

-

R

-

E

-

R

-

D

-

V

-

S

-

Y

F

S

E

H

V

C

L

L

T

L

C

N

Y

Y

P

K

G

G

M

F

H

L

A

A

I

C

W

Q

I

A

H

H

R

R

L

V

S

A

G

H

A

L

L

L

W

W

N

R

M

Q

G

S

W

R

K

R

W

P

L

L

A

A

R

L

L

A

V

L

S

H

N

S

F

R

G

I

R

A

W

N

L

V

T

F

G

A

I

V

E

D

I

I

H

H

P

P

G

A

A

A

K

R

V

I

G

G

R

K

R

G

F

I

F

L

I

F

D

D

H
|
H

G

A

M

T

G

G

I

V

V

V

I

V

G

G

E

E

T

T

A

A

E

V

I

I

G

G

D

N

D

N

V

V

T

S

L

I

Y
x
L

Q

H

G

H

V

V

T

T

L

L

G

G

G
|
G

T

T

S

G

W

-

N

-

K

-

G

G

K

D

R

R

H

H

P

P

T

K

L

I

E

G

D

D

G

G

V

V

V

L

V

I

G

G

A

A

G

G

A

A

K

T

V

I

L

L

G

G

P

N

F

I

T

K

V

I

G

G

A

E

G

G

A

A

K
|
K

V

V

G
|
G

S
x
A

N

G

A

S

V

V

V

V

T
x
L

K

I

A

D

V

V

P

P

P

P

G

R

A

T

T

T

V

A

V
|
V

G

G

I

N

P

P

G

A

R

R

I

L

I

V

binding coenzyme a: H150 (= H82), L169 (≠ Y101), G176 (= G108), K208 (= K143), G210 (= G145), A211 (≠ S146), L216 (≠ T151), V226 (= V161)

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
36% identity, 32% coverage: 85:167/258 of query aligns to 58:140/294 of 4mzuF

query
sites
4mzuF

G

G

I

V

V

Q

I

I

G

W

E

D

T

G

A

I

E

H

I

I

G

Q

D

D

D

D

V

V

T

F

L

I

Y

G

Q

P

G

N

V

V

T

T

L

F

G

T

G
x
N

T
x
D

S
x
K

W

Q

N

P

K

R

G

S

K

K

R

I

H

K

P

T

T

I

L

V

E

K

D

K

G

G

V

A

V

S

V

I

G
|
G

A
|
A

G

N

A

S

K

T

V

I

L
|
L

G

P

P

G

F

I

T

L

V

I

G

G

A

E

G

N

A

A

K

M

V

V

G
|
G

S
x
A

N

G

A

A

V

V

V

I

T
|
T

K
|
K

A

N

V

V

P

P

P

D

G

N

A

A

T

I

V

V

V
x
I

G

G

I
x
N

P

P

G

G

R
|
R

I

I

binding coenzyme a: N81 (≠ G108), D82 (≠ T109), K83 (≠ S110), G100 (= G127), A101 (= A128), L106 (= L133), G118 (= G145), A119 (≠ S146), T124 (= T151), K125 (= K152), I134 (≠ V161), N136 (≠ I163), R139 (= R166)

Sites not aligning to the query:

binding thymine: 20, 21, 38
binding thymidine-5'-diphosphate: 189, 203, 205, 270

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
29% identity, 37% coverage: 74:168/258 of query aligns to 77:181/201 of 1krvA

query
sites
1krvA

V

I

G

G

R

R

R

N

F

F

F
x
Y

I

A

D
x
N

H

F

G

N

M

L

G

T

I

I

V
|
V

I

D

G
x
D

E

Y

T

T

A

V

E

T

I

I

G

G

D

D

D

N

V

V

T

L

L

I

Y
x
A

Q

P

G

N

V

V

T

T

L

L

G
x
S

G

V

T
|
T

S

G

W

H

-

P

-

V

-

H

-

H

-

E

-

L

-

R

N

K

K

N

G

G

K

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

E

G

D

N

G

N

V

V

V
x
W

V

I

G
|
G

A
x
S

G

H

A

V

K

V

V

I

L
x
N

G

P

P

G

F

V

T

T

V

I

G

G

A

D

G

N

A

S

K

V

V

I

G

G

S
x
A

N

G

A

S

V

I

V

V

T

T

K

K

A

D

V

I

P

P

P

P

G

N

A

V

T

V

V

A

V

A

G

G

I

V

P
|
P

G

C

R
|
R

I

V

I

I

binding 4-nitrophenyl beta-D-galactopyranoside: Y82 (≠ F79), N84 (≠ D81), V90 (= V87), D92 (≠ G89), M126 (≠ R116)
binding coenzyme a: A104 (≠ Y101), S110 (≠ G107), T112 (= T109), W138 (≠ V125), G140 (= G127), S141 (≠ A128), N146 (≠ L133), A159 (≠ S146), P177 (= P164), R179 (= R166)

Sites not aligning to the query:

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
29% identity, 37% coverage: 74:168/258 of query aligns to 77:181/201 of 1kruA

query
sites
1kruA

V

I

G

G

R

R

R

N

F

F

F
x
Y

I

A

D
x
N

H

F

G

N

M

L

G

T

I

I

V
|
V

I

D

G
x
D

E

Y

T

T

A

V

E

T

I

I

G

G

D

D

D

N

V

V

T
x
L

L

I

Y

A

Q

P

G

N

V

V

T

T

L

L

G

S

G

V

T

T

S

G

W
x
H

-

P

-

V

-

H

-

H

-

E

-

L

-

R

N

K

K

N

G

G

K

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

E

G

D

N

G

N

V

V

V
x
W

V

I

G
|
G

A
x
S

G

H

A

V

K

V

V

I

L

N

G

P

P

G

F

V

T

T

V

I

G

G

A

D

G

N

A

S

K

V

V

I

G
|
G

S
x
A

N

G

A

S

V

I

V

V

T

T

K

K

A

D

V

I

P

P

P

P

G

N

A

V

T

V

V

A

V

A

G

G

I

V

P
|
P

G

C

R

R

I

V

I

I

binding coenzyme a: H114 (≠ W111), W138 (≠ V125), G140 (= G127), S141 (≠ A128), G158 (= G145), A159 (≠ S146), P177 (= P164)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: Y82 (≠ F79), Y82 (≠ F79), N84 (≠ D81), V90 (= V87), D92 (≠ G89), L102 (≠ T99), H114 (≠ W111), M126 (≠ R116)

Sites not aligning to the query:

G3XD01 UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronate N-acetyltransferase; UDP-D-GlcNAc3NA N-acetyltransferase; UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronic acid 3-N-acetyltransferase; EC 2.3.1.201 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
29% identity, 43% coverage: 58:168/258 of query aligns to 25:152/191 of G3XD01

query
sites
G3XD01

N

H

F

F

G

V

R

H

W

I

L

C

T

A

G

G

I

A

E

R

I

I

H

G

P

A

G

G

A

V

K

S

V

L

G

G

R

Q

R

N

F

V

F

F

I

V

D

-

H

-

G

G

M

N

G

K

I

V

V

V

I

I

G

G

E

D

T

R

A

C

E

K

I

I

G

Q

D

N

D

N

V

V

T

S

L

V

Y

Y

Q

D

G

N

V

V

T

T

L

L

-

E

-

E

-

G

-

V

-

F

G

C

G

G

T

P

S

S

W

M

-

V

-

F

-

T

-

N

-

V

-

Y

-

N

-

P

-

R

-

S

-

L

-

I

-

E

-

R

N

K

K

D

G

Q

K

Y

R

R

H

N

P

T

T

L

L

V

E

K

D

K

G

G

V

A

V

T

V

L

G

G

A

A

G

N

A

C

K

T

V

I

L

V

G

C

P

G

F

V

T

T

V

I

G

G

A

E

G

Y

A

A

K

F

V

V

G

G

S

A

N

G

A

A

V

V

V

I

T

N

K
|
K

A

N

V

V

P

P

P

S

G

Y

A

A

T

L

V

M

V

V

G

G

I

V

P

P

G

A

R

R

I

Q

I

I

K136 (= K152) mutation to A: Reduction in catalytic activity.; mutation to R: No effect on catalytic activity.

Query Sequence

>AO356_06655 FitnessBrowser__pseudo5_N2C3_1:AO356_06655
MFERLREDIQSVFHRDPAARNAFEVLTCYPGMHAIWIHRLSGALWNMGWKWLARLVSNFG
RWLTGIEIHPGAKVGRRFFIDHGMGIVIGETAEIGDDVTLYQGVTLGGTSWNKGKRHPTL
EDGVVVGAGAKVLGPFTVGAGAKVGSNAVVTKAVPPGATVVGIPGRIIVKSDEEQDARRK
AMAEKIGFDAYGVGEDMPDPVARAINQLLDHLQAVDGRLEGMCGALKDLGSNYCAKDLPE
LREEDFACVKDKDQSQAG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory