SitesBLAST

R49 (= R41) mutation to A: Less than 1% of the wild-type enzyme activity.
K60 (= K52) mutation to A: Less than 1% of the wild-type enzyme activity.
D69 (= D61) mutation to A: No effect on the enzyme activity.
R72 (= R64) mutation to A: Less than 1% of the wild-type enzyme activity.
T105 (≠ N97) mutation to A: 20% of the wild-type enzyme activity.
E109 (≠ Q101) mutation to A: 10% of the wild-type enzyme activity.; mutation to Q: 40% of the wild-type enzyme activity at pH 7.5 and 27% of the wild-type enzyme activity at pH 4.
R134 (= R127) mutation to A: Less than 1% of the wild-type enzyme activity.
C160 (= C153) modified: Disulfide link with 193
C193 (= C182) modified: Disulfide link with 160

Sites not aligning to the query:

1:33 signal peptide

2ao2B The 2.07 angstrom crystal structure of mycobacterium tuberculosis chorismate mutase reveals unexpected gene duplication and suggests a role in host-pathogen interactions (see paper)
32% identity, 81% coverage: 31:182/187 of query aligns to 5:159/165 of 2ao2B

query
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2ao2B

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active site: R15 (= R41), K26 (= K52), D35 (= D61), T71 (≠ N97), E72 (≠ K98), E75 (≠ Q101), R100 (= R127)
binding sulfate ion: K26 (= K52), R38 (= R64), Q42 (≠ V68)
binding tryptophan: L31 (≠ K57), P32 (= P58), I33 (≠ V59), E34 (= E60)

2ao2A The 2.07 angstrom crystal structure of mycobacterium tuberculosis chorismate mutase reveals unexpected gene duplication and suggests a role in host-pathogen interactions (see paper)
32% identity, 81% coverage: 31:182/187 of query aligns to 5:159/165 of 2ao2A

query
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2ao2A

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active site: R15 (= R41), K26 (= K52), D35 (= D61), T71 (≠ N97), E72 (≠ K98), E75 (≠ Q101), R100 (= R127)
binding sulfate ion: K26 (= K52), R38 (= R64), R100 (= R127)

2fp2B Secreted chorismate mutase from mycobacterium tuberculosis (see paper)
32% identity, 81% coverage: 31:182/187 of query aligns to 3:157/163 of 2fp2B

query
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2fp2B

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active site: R13 (= R41), K24 (= K52), D33 (= D61), T69 (≠ N97), E70 (≠ K98), E73 (≠ Q101), R98 (= R127)
binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R13 (= R41), K24 (= K52), I31 (≠ V59), E32 (= E60), D33 (= D61), R36 (= R64), Q40 (≠ V68), I66 (= I94), T69 (≠ N97), E70 (≠ K98), E73 (≠ Q101), R98 (= R127)

Query Sequence

>AO356_09710 FitnessBrowser__pseudo5_N2C3_1:AO356_09710
MTLRFKLCLALSAALLASTAAAASTPAPEALTPLLNAIGERLAIADQVALSKWDSGKPVE
DRQREREVIAAAVAQAPAYKLSNETVEAFFAAQIEANKMVQYINLSDWALDGKAPDLPRP
DLVGQIRPQLDRLQKRLLQQLADFAPYRTDPQCPQWLAHATHHDKQHPVHRLALIRATAE
LCISTKA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory