SitesBLAST

Q970U6 Glutamate--LysW ligase ArgX; EC 6.3.2.- from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
31% identity, 79% coverage: 52:289/301 of query aligns to 41:279/282 of Q970U6

query
sites
Q970U6

G

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D237 (= D248) binding Mg(2+)
E250 (= E260) binding Mg(2+); binding Mg(2+)
N252 (= N262) binding Mg(2+)

Q4JAP9 Alpha-aminoadipate--LysW ligase LysX; AAA--LysW ligase LysX; EC 6.3.2.43 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see paper)
29% identity, 69% coverage: 80:286/301 of query aligns to 62:270/276 of Q4JAP9

query
sites
Q4JAP9

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NT 253:254 (≠ GI 269:270) mutation to GF: Alters substrate specificity, so that glutamate is preferred over alpha-aminoadipate.

3vpdA Lysx from thermus thermophilus complexed with amp-pnp (see paper)
29% identity, 78% coverage: 51:285/301 of query aligns to 42:274/281 of 3vpdA

query
sites
3vpdA

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binding phosphoaminophosphonic acid-adenylate ester: V127 (= V139), K129 (= K141), W135 (≠ Q147), G136 (= G148), L139 (≠ V151), Q169 (= Q177), V172 (≠ I180), K174 (≠ A183), D178 (= D187), R194 (= R203), W199 (≠ F210), T201 (≠ S212), F239 (≠ L250), E249 (= E260)
binding butanoic acid: F256 (≠ L267), K257 (≠ E268), N258 (≠ G269), S259 (≠ I270)

5k2mI Bifunctional lysx/argx from thermococcus kodakarensis with lysw-gamma- aaa (see paper)
26% identity, 71% coverage: 71:285/301 of query aligns to 54:266/273 of 5k2mI

query
sites
5k2mI

F
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x
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binding adenosine-5'-diphosphate: K83 (= K100), V120 (= V139), K122 (= K141), S127 (≠ T146), L132 (≠ V151), Q162 (= Q177), V165 (≠ I180), K167 (≠ A183), D171 (= D187), W192 (≠ F210), I193 (≠ R211), T194 (≠ S212), N195 (= N213), F231 (≠ L250), N240 (≠ M259), E241 (= E260)
binding 2-aminohexanedioic acid: R173 (= R189), N195 (= N213), T196 (≠ L214), A197 (≠ H215), E247 (≠ G266), F248 (≠ L267), N250 (≠ G269)
binding : F54 (= F71), F125 (≠ E144), W128 (≠ Q147), R130 (≠ I149), N153 (≠ Q172), Y156 (≠ M175), N245 (≠ S264)

Sites not aligning to the query:

binding : 51, 52, 53

5k2mG Bifunctional lysx/argx from thermococcus kodakarensis with lysw-gamma- aaa (see paper)
26% identity, 71% coverage: 71:285/301 of query aligns to 54:266/273 of 5k2mG

query
sites
5k2mG

F
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x
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E

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P

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G
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L
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F

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I
x
A

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Y

binding adenosine-5'-diphosphate: K83 (= K100), V120 (= V139), K122 (= K141), S127 (≠ T146), G129 (= G148), Q162 (= Q177), V165 (≠ I180), K167 (≠ A183), D171 (= D187), W192 (≠ F210), T194 (≠ S212), N195 (= N213), F231 (≠ L250), N240 (≠ M259), E241 (= E260)
binding magnesium ion: D229 (= D248), E241 (= E260)
binding : F54 (= F71), F125 (≠ E144), S127 (≠ T146), W128 (≠ Q147), R130 (≠ I149), Y156 (≠ M175), R173 (= R189), R187 (= R203), T196 (≠ L214), A197 (≠ H215), N243 (= N262), P244 (≠ S263), N245 (≠ S264), E247 (≠ G266), F248 (≠ L267), N250 (≠ G269), A251 (≠ I270)

Sites not aligning to the query:

binding : 51, 52, 53

1uc9A Crystal structure of a lysine biosynthesis enzyme, lysx, from thermus thermophilus hb8 (see paper)
28% identity, 78% coverage: 51:285/301 of query aligns to 42:250/256 of 1uc9A

query
sites
1uc9A

R

R

G

P

K

K

P

E

L

L

E

E

G

G

F

V

D

T

A

V

V

A

I

L

P

E

R

R

I

C

G

V

A

S

S

Q

V

S

T

R

F

-

Y

-

G

G

C

L

A

A

V

A

L

A

R

R

Q

Y

F

L

E

T

M

A

M

L

G

G

V

I

F

P

P

V

L

V

N

N

E

R

S

P

V

E

A

V

I

I

A

E

R

A

S

C

R

G

D

D

K

K

L

W

R

A

S

T

L

S

Q

V

L

A

L

L

S

A

R

K

R

A

G

G

I

L

G

P

L

Q

P

P

V

K

T

T

G

A

F

L

A

A

H

T

S

D

P

R

D

E

I

A

P

L

D

R

L

L

I

M

A

E

M

A

V

F

N

-

G

G

A

Y

P

P

L

V

V

V

I

L

K
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K

V

P

L

V

E

I

G

G

T

A

Q

A

G

-

I

-

G

-

V

-

L

-

C

-

E

A

T

A

A

A

T

A

A

A

E

A

S

A

V

A

I

A

E

A

A

A

F

A

M

A

G

G

L

F

K

Q

Q

H

N

Q

I

L

M

F

-

Y

V

I

Q

Q

E

E

Y
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Y

I
x
V

K

-

E

E

A

K

G

P

G

G

A

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D
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D

I

I

R

R

C

V

F

F

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V

G

G

D

E

K

R

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A

I

I

A

A

S

A

M

I

K

Y

R
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R

Q

-

A

-

K

-

P

-

G

-

E

-

F

-

R

-

S

-

N

-

L

-

H

-

R

-

G

-

S

-

A

A

S

E

L

N

I

C

K

P

I

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T

T

P

E

E

E

E

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R

A

M

R

T

L

A

S

L

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R

K

A

A

K

E

V

A

M

V

G

G

L

G

A

G

V

V

A

V

G

A

V

V

D

D

I

L

L
x
F

R

E

S

S

N

E

H

R

G

G

P

L

L

L

V

V

M
x
N

E

E

V

V

N

N

S

H

S

T

P

M

G

E

L

F

E

K

G

N

I

S

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T

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T

T

S

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G

I

E

I

I

I

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E

K

H

Y

binding adenosine-5'-diphosphate: K129 (= K141), Y161 (= Y179), V162 (≠ I180), D168 (= D187), R184 (= R203), F215 (≠ L250), N224 (≠ M259)

7drpA Structure of atp-grasp ligase psnb complexed with phosphomimetic variant of minimal precursor, mg, and adp (see paper)
30% identity, 34% coverage: 185:286/301 of query aligns to 207:306/312 of 7drpA

query
sites
7drpA

G

G

A

D

D
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D

I

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R

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Y

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A

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D

I

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x
K

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R

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M
x
L

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E

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L

N
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N

S

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S
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S

P
x
A

G
x
M

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R

G

G

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L

binding adenosine-5'-diphosphate: D209 (= D187), K269 (≠ L250), L279 (≠ M259), E280 (= E260)
binding magnesium ion: D267 (= D248), E280 (= E260), E280 (= E260), N282 (= N262)
binding : R211 (= R189), N282 (= N262), S284 (= S264), A285 (≠ P265), M286 (≠ G266)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 170, 178, 202, 203, 205
binding : 70, 77, 79, 169, 175, 176, 179, 190, 193, 194, 199

7drnA Structure of atp-grasp ligase psnb complexed with precursor peptide psna2 and amppnp (see paper)
30% identity, 34% coverage: 185:286/301 of query aligns to 207:306/311 of 7drnA

query
sites
7drnA

G

G

A

D

D
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D

I

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R
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R

C

V

F

Y

V

V

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Q

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V

I

I

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C

S

A

M

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-

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A

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V

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G

L

L

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Y

A

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x
K

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A

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N

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Y

-

R

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M
x
L

E
|
E

V

L

N

N

S

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S
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S

P

A

G
x
M

L

F

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R

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L

binding phosphoaminophosphonic acid-adenylate ester: D209 (= D187), K269 (≠ L250), L279 (≠ M259), E280 (= E260)
binding : R211 (= R189), S284 (= S264), M286 (≠ G266)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 170, 178, 202, 203, 205
binding : 70, 79, 169, 171, 175, 176, 179, 190

7lg5A Synechocystis sp. Utex2470 cyanophycin synthetase 1 with atp (see paper)
23% identity, 65% coverage: 99:293/301 of query aligns to 219:482/871 of 7lg5A

query
sites
7lg5A

D

D

K

K

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I

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Q

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D

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E

D

E

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A

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N

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Y

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L

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V
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V

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I

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K

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D

G

G

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N

Q

H

G

G

I

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G

G

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V

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L

I

C

-

E

N

T

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A

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A

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A

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I

I

A

E

A

A

Y

F

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M

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G

A

L

A

K

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Q

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N

S

I

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V

Q

E

E

R

Y

Y

I
x
Y

K

E

E

-

A

-

G

-

G

G

A

S

D
|
D

I

H

R

R

C

V

F

L

V

V

G

N

D

G

K

K

V

L

I

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R

-

I

-

P

-

A

-

H

-

V

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T

-

G

-

D

-

G

-

S

-

T

-

I

-

S

-

E

-

L

-

I

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K

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-

N

-

Q

Q

D

A

P

K

N

P

R

G

G

E

D

F

G

R

H

S

D

N

N

-

I

-

L

-

T

-

K

-

I

-

V

-

N

-

K

-

T

-

A

-

I

-

D

-

V

L

M

H

E

R

R

G

Q

G

G

S

Y

A

-

S

N

L

L

I

D

K

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I

V

T

L

P

P

E

K

E

D

R

E

M

V

T

V

A

Y

L

L

R

R

A

A

-

T

-

A

-

N

-

L

-

S

-

T

-

G

-

I

-

A

-

I

-

D

-

R

-

T

-

D

-

I

-

H

-

P

-

E

-

N

-

I

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W

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L

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M

-

E

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R

-

V

A

A

K

K

V

V

M

I

G

G

L

L

A

D

V

I

A

A

G

G

V

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D
|
D

I

V

-

V

-

T

-

S

-

D

-

I

-

S

-

K

-

P

L

L

R

R

S

E

N

T

H

N

G

G

P

-

L

V

V

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M

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E
|
E

V

V

N

N

S

A

P

P

G

G

L

F

E

R

-

M

-

H

-

V

G

A

I

P

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S

T

Q

T

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T

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R

N

N

V

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G

A

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I

V

I

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E

D

H

M

L

L

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P

G

G

G

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P

N

S

binding adenosine-5'-triphosphate: V259 (= V139), Y299 (≠ I180), D303 (= D187)
binding magnesium ion: D427 (= D248), E446 (= E260)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 493, 494, 496, 692, 727, 741
binding magnesium ion: 496, 518, 554

3r5xD Crystal structure of d-alanine--d-alanine ligase from bacillus anthracis complexed with atp
26% identity, 44% coverage: 135:267/301 of query aligns to 132:269/306 of 3r5xD

query
sites
3r5xD

G

G

A

F

P

P

L

L

V
|
V

I

V

K
|
K

V

P

L

N

E

S

G

G

T

G

Q
x
S

G

S

I

V

G

G

V
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V

V

K

L

I

C

V

E

Y

T

D

A

K

T

D

A

E

A

L

E

I

S

S

V

M

I

L

E

E

A

T

F

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M

F

G

E

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W

K

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S

N

E

I

V

M

V

V

I

Q
x
E

E

K

Y
|
Y

I
|
I

K

K

E

-

A

-

G

-

G

G

A

E

D
x
E

I

I

R

T

C

C

F

S

V

I

V

F

G

D

D

G

K

K

V

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-

L

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P

I

I

A

I

S

S

M

I

K

R

R

H

Q

A

A

A

K
x
E

P

F

G

F

E
x
D

F

Y

R
x
N

S

A

N

K

L
x
Y

H

D

R

D

G

A

G

S

S

T

A

I

S

E

L

E

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K

I

I

E

T

L

P

P

E

A

E

E

-

L

-

K

-

E

-

R

-

V

R

N

M

K

T

A

A

S

L

L

R

A

A

C

A

Y

K

K

V

A

M

L

G

K

L

C

A

S

V

V

-

Y

A

A

G
x
R

V

V

D
|
D

I

M

L
x
M

R

V

S

K

N

D

H

G

G

I

P

P

L

Y

V

V

M
|
M

E
|
E

V

V

N
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N

S

T

S

L

P

P

G
|
G

L

M

active site: S144 (≠ Q147), N207 (≠ R211), Y210 (≠ L214), R248 (≠ G246), D250 (= D248), E262 (= E260), N264 (= N262), G268 (= G266)
binding adenosine-5'-triphosphate: V136 (= V139), K138 (= K141), V148 (= V151), E174 (≠ Q177), Y176 (= Y179), I177 (= I180), E181 (≠ D187), M252 (≠ L250), M261 (= M259), E262 (= E260), N264 (= N262)
binding calcium ion: E202 (≠ K206), D205 (≠ E209), Y210 (≠ L214), D250 (= D248), E262 (= E260)

Sites not aligning to the query:

active site: 15, 18, 21, 63, 272

Query Sequence

>AO356_10160 FitnessBrowser__pseudo5_N2C3_1:AO356_10160
MKIAVLSRNPRLYSTRRLVEAGTERGHEMVVIDTLRAYMNIASHKPQIHYRGKPLEGFDA
VIPRIGASVTFYGCAVLRQFEMMGVFPLNESVAIARSRDKLRSLQLLSRRGIGLPVTGFA
HSPDDIPDLIAMVNGAPLVIKVLEGTQGIGVVLCETATAAESVIEAFMGLKQNIMVQEYI
KEAGGADIRCFVVGDKVIASMKRQAKPGEFRSNLHRGGSASLIKITPEERMTALRAAKVM
GLAVAGVDILRSNHGPLVMEVNSSPGLEGIETTTSKNVAGIIIEHLEKNGGPNMTRTKGK
G

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory