SitesBLAST
Comparing AO356_12630 FitnessBrowser__pseudo5_N2C3_1:AO356_12630 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1rcqA The 1.45 a crystal structure of alanine racemase from a pathogenic bacterium, pseudomonas aeruginosa, contains both internal and external aldimine forms (see paper)
74% identity, 100% coverage: 1:356/357 of query aligns to 1:356/357 of 1rcqA
- active site: K33 (= K33), R129 (= R129), H158 (= H158), R208 (= R208), Y253 (= Y253), S300 (= S300), D302 (= D302)
- binding d-lysine: K33 (= K33), R129 (= R129), Y341 (= Y341)
- binding pyridoxal-5'-phosphate: V31 (= V31), K33 (= K33), Y37 (= Y37), H158 (= H158), N192 (= N192), S193 (= S193), R208 (= R208), G210 (= G210), I211 (= I211), Y341 (= Y341)
Q9HTQ2 Alanine racemase, catabolic; EC 5.1.1.1 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
74% identity, 100% coverage: 1:356/357 of query aligns to 1:356/357 of Q9HTQ2
- K33 (= K33) modified: N6-(pyridoxal phosphate)lysine
- K122 (= K122) modified: N6-carboxylysine
2rjhA Crystal structure of biosynthetic alaine racemase in d-cycloserine- bound form from escherichia coli (see paper)
49% identity, 97% coverage: 1:347/357 of query aligns to 1:349/359 of 2rjhA
- active site: K34 (= K33), R129 (= R129), H159 (= H158), R209 (= R208), Y255 (= Y253), A302 (≠ S300), D304 (= D302)
- binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: K34 (= K33), Y38 (= Y37), R129 (= R129), H159 (= H158), A193 (≠ N192), S194 (= S193), R209 (= R208), G211 (= G210), I212 (= I211), Y255 (= Y253), A302 (≠ S300), M303 (= M301), Y343 (= Y341)
P0A6B4 Alanine racemase, biosynthetic; EC 5.1.1.1 from Escherichia coli (strain K12) (see paper)
49% identity, 97% coverage: 1:347/357 of query aligns to 1:349/359 of P0A6B4
- K34 (= K33) modified: N6-(pyridoxal phosphate)lysine
- K122 (= K122) modified: N6-carboxylysine
- D164 (= D163) mutation to A: Slightly reduces affinity for D-Ala and L-Ala.; mutation to K: Reduces catalytic activity. Slightly reduces affinity for D-Ala and L-Ala.
- E165 (= E164) mutation to A: Slightly reduces affinity for D-Ala and L-Ala.; mutation to K: Reduces catalytic activity. Slightly reduces affinity for D-Ala and L-Ala.
- P219 (= P218) mutation to A: No effect on catalytic activity. No effect on affinity for D-Ala and L-Ala.
- E221 (≠ D220) mutation E->A,K,P: Slightly increases catalytic activity. Slightly increases affinity for D-Ala and L-Ala.
3b8tA Crystal structure of escherichia coli alaine racemase mutant p219a (see paper)
48% identity, 97% coverage: 1:347/357 of query aligns to 1:349/359 of 3b8tA
- active site: K34 (= K33), R129 (= R129), H159 (= H158), R209 (= R208), Y255 (= Y253), A302 (≠ S300), D304 (= D302)
- binding pyridoxal-5'-phosphate: V32 (= V31), K34 (= K33), Y38 (= Y37), L78 (= L78), H159 (= H158), A193 (≠ N192), S194 (= S193), R209 (= R208), G211 (= G210), I212 (= I211), Y343 (= Y341)
4xbjA Y274f alanine racemase from e. Coli inhibited by l-ala-p (see paper)
49% identity, 96% coverage: 4:347/357 of query aligns to 2:347/357 of 4xbjA
- active site: K32 (= K33), R127 (= R129), H157 (= H158), R207 (= R208), Y253 (= Y253), A300 (≠ S300), D302 (= D302)
- binding {1-[(3-hydroxy-methyl-5-phosphonooxy-methyl-pyridin-4-ylmethyl)-amino]-ethyl}-phosphonic acid: V30 (= V31), K32 (= K33), Y36 (= Y37), R127 (= R129), H157 (= H158), A191 (≠ N192), S192 (= S193), R207 (= R208), G209 (= G210), I210 (= I211), Y253 (= Y253), A300 (≠ S300), M301 (= M301), Y341 (= Y341)
6a2fA Crystal structure of biosynthetic alanine racemase from pseudomonas aeruginosa (see paper)
49% identity, 99% coverage: 1:355/357 of query aligns to 1:357/358 of 6a2fA
- active site: K34 (= K33), R130 (= R129), H159 (= H158), R209 (= R208), Y254 (= Y253), S301 (= S300), D303 (= D302)
- binding acetate ion: Y254 (= Y253), R279 (= R278), L340 (≠ I339), Y342 (= Y341)
- binding d-lysine: Y254 (= Y253), Y273 (= Y272), S301 (= S300), M302 (= M301), D303 (= D302), M304 (= M303)
- binding malonate ion: K123 (= K122), R130 (= R129), L131 (≠ V130)
P0A2W8 Alanine racemase; EC 5.1.1.1 from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (see paper)
38% identity, 99% coverage: 2:354/357 of query aligns to 7:366/367 of P0A2W8
- K129 (= K122) modified: N6-carboxylysine
4y2wA Crystal structure of a thermostable alanine racemase from thermoanaerobacter tengcongensis mb4 (see paper)
34% identity, 99% coverage: 1:354/357 of query aligns to 6:374/388 of 4y2wA
- active site: K40 (= K33), R138 (= R129), H168 (= H158), R224 (= R208), Y268 (= Y253), C315 (≠ S300), D317 (= D302)
- binding alanine: Y268 (= Y253), Y287 (= Y272), C315 (≠ S300), M316 (= M301)
- binding phosphate ion: N208 (= N192), A209 (≠ S193), I227 (= I211)
3e5pB Crystal structure of alanine racemase from e.Faecalis (see paper)
35% identity, 99% coverage: 2:355/357 of query aligns to 7:371/371 of 3e5pB
- active site: K40 (= K33), R139 (= R129), H169 (= H158), R222 (= R208), Y267 (= Y253), C313 (≠ S300), D315 (= D302)
- binding pyridoxal-5'-phosphate: K40 (= K33), Y44 (= Y37), R139 (= R129), H169 (= H158), S207 (= S193), G224 (= G210), V225 (≠ I211), Y356 (= Y341)
3e6eA Crystal structure of alanine racemase from e.Faecalis complex with cycloserine (see paper)
35% identity, 99% coverage: 2:355/357 of query aligns to 6:370/370 of 3e6eA
- active site: K39 (= K33), R138 (= R129), H168 (= H158), R221 (= R208), Y266 (= Y253), C312 (≠ S300), D314 (= D302)
- binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: K39 (= K33), Y43 (= Y37), L85 (= L78), R138 (= R129), H168 (= H158), S206 (= S193), R221 (= R208), G223 (= G210), V224 (≠ I211), Y266 (= Y253), Y285 (= Y272), M313 (= M301), Y355 (= Y341)
5fagA Alanine racemase from streptomyces coelicolor a3(2) with bound propionate inhibitor (see paper)
39% identity, 98% coverage: 4:354/357 of query aligns to 15:387/390 of 5fagA
- active site: I19 (= I8), K45 (= K33), A46 (= A34), R147 (= R129), H180 (= H158), R236 (= R208), Y282 (= Y253), A329 (≠ S300), D331 (= D302)
- binding pyridoxal-5'-phosphate: K45 (= K33), Y49 (= Y37), W95 (≠ G80), W178 (≠ M156), H180 (= H158), N220 (= N192), S221 (= S193), R236 (= R208), G238 (= G210), I239 (= I211), Y373 (= Y341)
5fajA Alanine racemase from streptomyces coelicolor a3(2) in complex with d- cycloserine (see paper)
39% identity, 98% coverage: 4:354/357 of query aligns to 10:382/385 of 5fajA
- active site: I14 (= I8), K40 (= K33), A41 (= A34), R142 (= R129), H175 (= H158), R231 (= R208), Y277 (= Y253), A324 (≠ S300), D326 (= D302)
- binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: K40 (= K33), Y44 (= Y37), W90 (≠ G80), W173 (≠ M156), H175 (= H158), N215 (= N192), S216 (= S193), R231 (= R208), P232 (= P209), G233 (= G210), I234 (= I211), Y277 (= Y253), Y296 (= Y272), M325 (= M301), Y368 (= Y341)
1vfhA Crystal structure of alanine racemase from d-cycloserine producing streptomyces lavendulae (see paper)
38% identity, 99% coverage: 6:357/357 of query aligns to 8:376/382 of 1vfhA
- active site: K36 (= K33), R134 (= R129), H166 (= H158), R222 (= R208), Y268 (= Y253), A315 (≠ S300), D317 (= D302)
- binding pyridoxal-5'-phosphate: K36 (= K33), Y40 (= Y37), W82 (≠ L78), H166 (= H158), S207 (= S193), R222 (= R208), G224 (= G210), L225 (≠ I211), Y359 (= Y341)
1vfsA Crystal structure of d-cycloserine-bound form of alanine racemase from d-cycloserine-producing streptomyces lavendulae (see paper)
38% identity, 99% coverage: 6:357/357 of query aligns to 8:376/383 of 1vfsA
- binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: K36 (= K33), Y40 (= Y37), W82 (≠ L78), R134 (= R129), H166 (= H158), S207 (= S193), R222 (= R208), G224 (= G210), Y268 (= Y253), Y287 (= Y272), M316 (= M301), Y359 (= Y341)
7xllA Alanine racemase from lactobacillus sakei uonuma-1. (see paper)
35% identity, 100% coverage: 1:356/357 of query aligns to 6:376/378 of 7xllA
Sites not aligning to the query:
P9WQA9 Alanine racemase; EC 5.1.1.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
36% identity, 98% coverage: 4:354/357 of query aligns to 12:378/384 of P9WQA9
- K42 (= K33) modified: N6-(pyridoxal phosphate)lysine
6sczA Mycobacterium tuberculosis alanine racemase inhibited by dcs (see paper)
36% identity, 98% coverage: 4:354/357 of query aligns to 4:370/373 of 6sczA
- active site: K34 (= K33), R132 (= R129), H164 (= H158), R220 (= R208), Y263 (= Y253), C310 (≠ S300), D312 (= D302)
- binding (~{E})-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylidene-[(4~{R})-3-oxidanylidene-1,2-oxazolidin-4-yl]azanium: K34 (= K33), Y38 (= Y37), W80 (≠ L78), H164 (= H158), S205 (= S193), R220 (= R208), G222 (= G210), I223 (= I211), Y356 (= Y341)
- binding [2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methyl-(3-oxidanyl-1,2-oxazol-4-yl)azanium: Y38 (= Y37), W80 (≠ L78), H164 (= H158), N204 (= N192), S205 (= S193), R220 (= R208), G222 (= G210), I223 (= I211), Y263 (= Y253), Y282 (= Y272), M311 (= M301), Y356 (= Y341)
- binding polyethylene glycol: D172 (≠ H166), A210 (≠ G198), P212 (= P200)
6g58A Structure of the alanine racemase from staphylococcus aureus in complex with a pyridoxal 5' phosphate-derivative (see paper)
35% identity, 100% coverage: 2:357/357 of query aligns to 7:371/382 of 6g58A
- active site: K39 (= K33), R138 (= R129), H168 (= H158), R219 (= R208), Y265 (= Y253), C311 (≠ S300), D313 (= D302)
- binding (6-but-3-ynyl-4-methyl-5-oxidanyl-pyridin-3-yl)methyl dihydrogen phosphate: K39 (= K33), Y43 (= Y37), L85 (= L78), K131 (= K122), R138 (= R129), H168 (= H158), S204 (= S193), R219 (= R208), G221 (= G210), I222 (= I211), Y354 (= Y341)
6g59A Structure of the alanine racemase from staphylococcus aureus in complex with an pyridoxal-6- phosphate derivative (see paper)
35% identity, 100% coverage: 2:357/357 of query aligns to 12:376/387 of 6g59A
- active site: K44 (= K33), R143 (= R129), H173 (= H158), R224 (= R208), Y270 (= Y253), C316 (≠ S300), D318 (= D302)
- binding (6-ethynyl-4-methanoyl-5-oxidanyl-pyridin-3-yl)methyl dihydrogen phosphate: K44 (= K33), Y48 (= Y37), L90 (= L78), H173 (= H158), S209 (= S193), R224 (= R208), G226 (= G210), I227 (= I211), Y359 (= Y341)
Query Sequence
>AO356_12630 FitnessBrowser__pseudo5_N2C3_1:AO356_12630
MRPARALIDLQALRHNYQLAREVTGAKALAVIKADAYGHGAVRCAEALQATADGFAVACI
EEALELRAGGIRGPILLLEGFFEADELPLIVEHDFWCVVHSLWQLEAIEKAALSQPITVW
LKLDSGMHRVGLHPADYQAAYRRLLASGKVAKIVLMSHFARADELHDPSSTEQLVVFEAA
RQGLAAEISLRNSPAVLGWPQMPSDWVRPGIMLYGATPFDEPNTVASRLQPVMTLESKII
CVRELPAGEPVGYGARFVTTQPTRVGVVAMGYADGYPRQAPNGTPVRVDGQRSQLVGRVS
MDMLCVDLTHVPQAGLGSTVELWGKNILASDVAKAADTIPYQIFCNLRRVPRLYSGG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory