SitesBLAST
Comparing AO356_14230 FitnessBrowser__pseudo5_N2C3_1:AO356_14230 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5yb6A L-amino acid oxidase/monooxygenase from pseudomonas sp. Aiu 813 - l- lysine complex (see paper)
92% identity, 99% coverage: 5:560/560 of query aligns to 1:556/556 of 5yb6A
- active site: E241 (= E245), K361 (= K365)
- binding flavin-adenine dinucleotide: V47 (= V51), G48 (= G52), G50 (= G54), I51 (= I55), A52 (= A56), Y70 (= Y74), E71 (= E75), A72 (= A76), S73 (= S77), G77 (= G81), R78 (= R82), L79 (= L83), G95 (= G99), G96 (= G100), M97 (= M101), R98 (= R102), F99 (= F103), G296 (= G300), V297 (= V301), T326 (= T330), C327 (= C331), L331 (= L335), I336 (= I340), S359 (= S363), Y412 (= Y416), W459 (= W463), H464 (= H468), F465 (= F469), A468 (= A472), F469 (= F473), G503 (= G507), D504 (= D508), A511 (= A515), W512 (= W516), V513 (= V517), A516 (= A520)
- binding lysine: R98 (= R102), E136 (= E140), W231 (= W235), D234 (= D238), T333 (= T337), E340 (= E344), Q345 (= Q349), W348 (= W352), D352 (= D356), M357 (= M361), D389 (= D393), R390 (= R394), L391 (= L395), Y412 (= Y416), W414 (= W418), F469 (= F473), A511 (= A515), W512 (= W516)
5yb8A L-amino acid oxidase/monooxygenase from pseudomonas sp. Aiu 813 - l- arginine complex (see paper)
92% identity, 98% coverage: 12:560/560 of query aligns to 1:549/549 of 5yb8A
- active site: E234 (= E245), K354 (= K365)
- binding arginine: R91 (= R102), G130 (= G141), D227 (= D238), E333 (= E344), Q338 (= Q349), W341 (= W352), D345 (= D356), D382 (= D393), R383 (= R394), L384 (= L395), Y405 (= Y416), W407 (= W418), F462 (= F473), A504 (= A515), W505 (= W516)
- binding flavin-adenine dinucleotide: V40 (= V51), G41 (= G52), A42 (= A53), G43 (= G54), I44 (= I55), A45 (= A56), Y63 (= Y74), E64 (= E75), A65 (= A76), S66 (= S77), G70 (= G81), R71 (= R82), L72 (= L83), G88 (= G99), G89 (= G100), M90 (= M101), R91 (= R102), F92 (= F103), G289 (= G300), V290 (= V301), T319 (= T330), C320 (= C331), L324 (= L335), I329 (= I340), S352 (= S363), K354 (= K365), Y405 (= Y416), W452 (= W463), H457 (= H468), F458 (= F469), A461 (= A472), F462 (= F473), G496 (= G507), D497 (= D508), A504 (= A515), W505 (= W516), V506 (= V517), A509 (= A520)
5yb7A L-amino acid oxidase/monooxygenase from pseudomonas sp. Aiu 813 - l- ornithine complex (see paper)
92% identity, 98% coverage: 12:560/560 of query aligns to 1:549/549 of 5yb7A
- active site: E234 (= E245), K354 (= K365)
- binding flavin-adenine dinucleotide: V40 (= V51), G41 (= G52), G43 (= G54), I44 (= I55), A45 (= A56), Y63 (= Y74), E64 (= E75), A65 (= A76), S66 (= S77), G70 (= G81), R71 (= R82), L72 (= L83), G88 (= G99), G89 (= G100), M90 (= M101), R91 (= R102), F92 (= F103), G289 (= G300), V290 (= V301), T319 (= T330), C320 (= C331), L324 (= L335), I329 (= I340), S352 (= S363), Y405 (= Y416), W452 (= W463), H457 (= H468), F458 (= F469), A461 (= A472), F462 (= F473), G496 (= G507), D497 (= D508), A504 (= A515), W505 (= W516), V506 (= V517), A509 (= A520)
- binding L-ornithine: R91 (= R102), D227 (= D238), T326 (= T337), E333 (= E344), Q338 (= Q349), W341 (= W352), D345 (= D356), R383 (= R394), L384 (= L395), Y405 (= Y416), W407 (= W418), F462 (= F473), A504 (= A515), W505 (= W516)
4iv9A Structure of the flavoprotein tryptophan-2-monooxygenase (see paper)
32% identity, 92% coverage: 30:544/560 of query aligns to 18:542/552 of 4iv9A
- active site: E249 (= E245), K360 (= K365)
- binding flavin-adenine dinucleotide: V39 (= V51), G40 (= G52), A41 (= A53), G42 (= G54), I43 (= I55), S44 (≠ A56), Y63 (= Y74), E64 (= E75), S65 (≠ A76), R66 (vs. gap), G71 (= G81), R72 (= R82), V73 (≠ L83), G90 (= G99), A91 (≠ G100), M92 (= M101), R93 (= R102), F94 (= F103), R294 (≠ G300), V295 (= V301), T323 (= T330), S324 (≠ C331), H333 (≠ E341), S358 (= S363), W461 (= W463), H466 (= H468), A470 (= A472), F471 (= F473), G505 (= G507), C506 (≠ D508), G513 (≠ A515), W514 (= W516), I515 (≠ V517), A518 (= A520)
- binding 2-(1h-indol-3-yl)acetamide: R93 (= R102), F239 (≠ W235), V242 (≠ D238), M253 (≠ V249), W410 (= W418), F471 (= F473), L473 (≠ G475), G512 (≠ P514), G513 (≠ A515), W514 (= W516)
P06617 Tryptophan 2-monooxygenase; EC 1.13.12.3 from Pseudomonas savastanoi (Pseudomonas syringae pv. savastanoi) (see paper)
32% identity, 92% coverage: 30:544/560 of query aligns to 23:547/557 of P06617
- K365 (= K365) mutation to M: Abolishes catalytic activity.
- W466 (= W463) mutation to F: Slightly decreases enzyme activity.; mutation to M: Abolishes flavin binding.
Q96RQ9 L-amino-acid oxidase; LAAO; LAO; Interleukin-4-induced protein 1; IL4-induced protein 1; hIL4I1; Protein Fig-1; hFIG1; EC 1.4.3.2; EC 1.4.3.25 from Homo sapiens (Human) (see 4 papers)
24% identity, 89% coverage: 47:544/560 of query aligns to 61:517/567 of Q96RQ9
- N92 (≠ S77) to D: increased L-amino-acid oxidase activity; dbSNP:rs111772144
- R102 (≠ A87) to G: in an ovarian cancer; decreased L-amino-acid oxidase activity
- N220 (≠ A213) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- E481 (≠ D508) mutation to A: Abolished L-amino-acid oxidase activity.
Sites not aligning to the query:
G8XQX1 L-amino-acid oxidase; DrLAO; LAAO; EC 1.4.3.2 from Daboia russelii (Russel's viper) (Vipera russelii) (see paper)
24% identity, 93% coverage: 9:527/560 of query aligns to 19:494/504 of G8XQX1
- H241 (vs. gap) mutation to A: Shows high reactivity toward L-Arg, but does not induce change toward L-Leu, L-Phe and L-Met.; mutation to N: No change in activity.; mutation to S: Shows middle reactivity toward L-Arg and L-Phe, but does not induce change toward L-Leu, and L-Met.
Sites not aligning to the query:
Q6STF1 L-amino-acid oxidase; AHP-LAAO; LAO; EC 1.4.3.2 from Gloydius halys (Chinese water mocassin) (Agkistrodon halys) (see paper)
22% identity, 88% coverage: 39:533/560 of query aligns to 45:500/504 of Q6STF1
- MS 61:62 (≠ IA 55:56) binding
- EA 81:82 (= EA 75:76) binding
- R89 (= R82) binding
- GPMR 105:108 (≠ GGMR 99:102) binding
- R108 (= R102) binding
- N190 (≠ W192) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- C191 (≠ N193) modified: Disulfide link with 28
- H241 (vs. gap) binding
- V279 (= V301) binding
- C349 (≠ V370) modified: Disulfide link with 430
- N379 (≠ D406) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- Y390 (= Y416) binding
- C430 (≠ I454) modified: Disulfide link with 349
- E475 (≠ D508) binding
- GWIDST 482:487 (≠ AWVEGA 515:520) binding
Sites not aligning to the query:
- 1:18 signal peptide
- 28 modified: Disulfide link with 191
1tdoA L-amino acid oxidae from agkistrodon halys in complex with l- phenylalanine
23% identity, 88% coverage: 39:533/560 of query aligns to 25:480/484 of 1tdoA