SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO356_14385 AO356_14385 spermidine/putrescine ABC transporter ATP-binding protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
48% identity, 78% coverage: 14:304/374 of query aligns to 17:306/378 of P69874

query
sites
P69874

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C26 (≠ S23) mutation to A: Lower ATPase activity and transport efficiency.
F27 (≠ Y24) mutation to L: Lower ATPase activity and transport efficiency.
F45 (= F43) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ S52) mutation to T: Loss of ATPase activity and transport.
L60 (= L58) mutation to F: Lower ATPase activity and transport efficiency.
L76 (= L74) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V133) mutation to M: Loss of ATPase activity and transport.
D172 (= D170) mutation to N: Loss of ATPase activity and transport.
C276 (≠ V274) mutation to A: Lower ATPase activity and transport efficiency.
E297 (= E295) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
48% identity, 74% coverage: 20:297/374 of query aligns to 9:287/393 of P9WQI3

query
sites
P9WQI3

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H193 (= H204) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
53% identity, 59% coverage: 26:247/374 of query aligns to 17:244/375 of 2d62A

query
sites
2d62A

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binding pyrophosphate 2-: P40 (= P49), S41 (= S50), G42 (= G51), C43 (≠ S52), G44 (= G53), K45 (= K54), T46 (= T55), T47 (= T56)

1g291 Malk (see paper)
51% identity, 59% coverage: 26:247/374 of query aligns to 14:241/372 of 1g291

query
sites
1g291

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binding magnesium ion: D69 (≠ N81), E71 (vs. gap), K72 (vs. gap), K79 (≠ H85), D80 (≠ K86), Y228 (= Y234), D229 (≠ E235)
binding pyrophosphate 2-: P37 (= P49), S38 (= S50), G39 (= G51), C40 (≠ S52), G41 (= G53), K42 (= K54), T43 (= T55), T44 (= T56)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 341, 342, 359

2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
41% identity, 84% coverage: 15:330/374 of query aligns to 3:319/372 of 2awoA

query
sites
2awoA

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L

H

H

Q

K

R

R

L

L

G

G

V

R

T

T

V

M

V

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

G

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

V

I

A

V

V

V

F

L

H

D

Q

A

G

G

E

R

I

V

Q

A

Q

Q

I

V

A

G

P

K

P

P

R

L

T

E

L

L

Y

Y

E

H

E

Y

P

P

K

A

N

D

T

R

F

F

V

V

A

A

N

G

F

F

I

I

G

G

-

S

-

P

E

K

N

M

N

N

R

F

L

L

N

P

G

V

R

K

L

V

H

T

S

A

H

T

T

A

G

I

D

D

R

Q

C

V

L

Q

V

V

E

E

L

L

A

P

R

M

G

P

E

N

K

R

-

Q

-

V

-

W

-

L

-

P

V

V

E

E

A

S

L

R

A

D

V

V

N

Q

V

V

G

-

Q

-

P

-

G

G

E

A

P

N

V

M

T

S

L

L

S

G

I

I

R

R

P

P

E

E

R

H

V

L

S

L

L

P

N

S

G

D

V

I

S

A

E

D

Q

V

C

I

V

-

N

-

R

-

F

L

S

E

G

G

R

E

V

V

A

Q

E

V

F

V

I

E

Y

Q

L

L

G

G

D

N

H

E

V

T

R

Q

V

I

R

H

L

I

E

Q

V

I

binding adenosine-5'-diphosphate: W12 (≠ Y24), V17 (≠ I30), S37 (= S50), G38 (= G51), C39 (≠ S52), G40 (= G53), K41 (= K54), S42 (≠ T55), T43 (= T56)
binding magnesium ion: S42 (≠ T55), D157 (= D170)

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
41% identity, 84% coverage: 15:330/374 of query aligns to 3:319/374 of 2awnB

query
sites
2awnB

V

V

S

Q

F

L

R

Q

G

N

V

V

Q

T

K

K

S

A

Y
x
W

D

-

G

G

E

E

N

V

L

V

I
x
V

V

S

K

K

D

D

L

I

N

N

L

L

D

D

I

I

R

H

K

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

S

L

L

L

M

R

M

M

L

I

A

A

G

G

F

L

E

E

T

T

P

I

T

T

A

S

G

G

E

D

I

L

L

F

L

I

A

G

G

E

R

K

S

R

I

M

N

N

N

D

V

T

P

P

H

A

K

E

R

R

D

G

I

V

G

G

M

M

V

V

F

F

Q

Q

N

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

A

S

F

F

P

G

L

L

T

K

V

L

R

A

G

G

L

A

N

K

K

K

S

E

D

V

V

I

S

N

D

Q

R

R

V

V

K

N

R

Q

V

V

L

A

S

E

M

V

V

L

Q

Q

L

L

D

A

S

H

F

L

A

L

Q

D

R

R

Y

K

P

P

A

K

Q

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

F

A

E

E

P

P

Q

S

L

V

V

F

L

L

M

L

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

K

A

Q

A

L

L

R

R

E

V

H

Q

M

M

Q

R

M

I

E

E

I

I

K

S

H

R

L

L

H

H

Q

K

R

R

L

L

G

G

V

R

T

T

V

M

V

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

G

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

V

I

A

V

V

V

F

L

H

D

Q

A

G

G

E

R

I

V

Q

A

Q

Q

I

V

A

G

P

K

P

P

R

L

T

E

L

L

Y

Y

E

H

E

Y

P

P

K

A

N

D

T

R

F

F

V

V

A

A

N

G

F

F

I

I

G

G

-

S

-

P

E

K

N

M

N

N

R

F

L

L

N

P

G

V

R

K

L

V

H

T

S

A

H

T

T

A

G

I

D

D

R

Q

C

V

L

Q

V

V

E

E

L

L

A

P

R

M

G

P

E

N

K

R

-

Q

-

V

-

W

-

L

-

P

V

V

E

E

A

S

L

R

A

D

V

V

N

Q

V

V

G

-

Q

-

P

-

G

G

E

A

P

N

V

M

T

S

L

L

S

G

I

I

R

R

P

P

E

E

R

H

V

L

S

L

L

P

N

S

G

D

V

I

S

A

E

D

Q

V

C

I

V

-

N

-

R

-

F

L

S

E

G

G

R

E

V

V

A

Q

E

V

F

V

I

E

Y

Q

L

L

G

G

D

N

H

E

V

T

R

Q

V

I

R

H

L

I

E

Q

V

I

binding adenosine-5'-diphosphate: W12 (≠ Y24), V17 (≠ I30), S37 (= S50), G38 (= G51), C39 (≠ S52), G40 (= G53), K41 (= K54), S42 (≠ T55), T43 (= T56)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
41% identity, 84% coverage: 15:330/374 of query aligns to 1:317/367 of 1q12A

query
sites
1q12A

V

V

S

Q

F

L

R

Q

G

N

V

V

Q

T

K

K

S

A

Y
x
W

D

-

G

G

E

E

N

V

L

V

I
x
V

V

S

K

K

D

D

L

I

N

N

L

L

D

D

I

I

R

H

K

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

S

L

L

L

M

R

M

M

L

I

A

A

G

G

F

L

E

E

T

T

P

I

T

T

A

S

G

G

E

D

I

L

L

F

L

I

A

G

G

E

R

K

S

R

I

M

N

N

N

D

V

T

P

P

H

A

K

E

R

R

D

G

I

V

G

G

M

M

V

V

F

F

Q

Q

N

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

A

S

F

F

P

G

L

L

T

K

V

L

R

A

G

G

L

A

N

K

K

K

S

E

D

V

V

I

S

N

D

Q

R

R

V

V

K

N

R

Q

V

V

L

A

S

E

M

V

V

L

Q

Q

L

L

D

A

S

H

F

L

A

L

Q

D

R
|
R

Y

K

P

P

A
x
K

Q
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

F

A

E

E

P

P

Q

S

L

V

V

F

L

L

M

L

D

D

E

E

P

P

L

L

G

S

A
x
N

L

L

D

D

K

A

Q

A

L

L

R

R

E

V

H

Q

M

M

Q

R

M

I

E

E

I

I

K

S

H

R

L

L

H

H

Q

K

R

R

L

L

G

G

V

R

T

T

V

M

V

I

Y

Y

V

V

T

T

H
|
H

D

D

Q

Q

G

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

V

I

A

V

V

V

F

L

H

D

Q

A

G

G

E

R

I

V

Q

A

Q

Q

I

V

A

G

P

K

P

P

R

L

T

E

L

L

Y

Y

E

H

E

Y

P

P

K

A

N

D

T

R

F

F

V

V

A

A

N

G

F

F

I

I

G

G

-

S

-

P

E

K

N

M

N

N

R

F

L

L

N

P

G

V

R

K

L

V

H

T

S

A

H

T

T

A

G

I

D

D

R

Q

C

V

L

Q

V

V

E

E

L

L

A

P

R

M

G

P

E

N

K

R

-

Q

-

V

-

W

-

L

-

P

V

V

E

E

A

S

L

R

A

D

V

V

N

Q

V

V

G

-

Q

-

P

-

G

G

E

A

P

N

V

M

T

S

L

L

S

G

I

I

R

R

P

P

E

E

R

H

V

L

S

L

L

P

N

S

G

D

V

I

S

A

E

D

Q

V

C

I

V

-

N

-

R

-

F

L

S

E

G

G

R

E

V

V

A

Q

E

V

F

V

I

E

Y

Q

L

L

G

G

D

N

H

E

V

T

R

Q

V

I

R

H

L

I

E

Q

V

I

binding adenosine-5'-triphosphate: W10 (≠ Y24), V15 (≠ I30), S35 (= S50), G36 (= G51), C37 (≠ S52), G38 (= G53), K39 (= K54), S40 (≠ T55), T41 (= T56), R126 (= R141), K129 (≠ A144), A130 (≠ Q145), L131 (= L146), S132 (= S147), G133 (= G148), G134 (= G149), Q135 (= Q150), N160 (≠ A175), H189 (= H204)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
41% identity, 84% coverage: 15:330/374 of query aligns to 3:319/371 of 3puyA

query
sites
3puyA

V

V

S

Q

F

L

R

Q

G

N

V

V

Q

T

K

K

S

A

Y
x
W

D

-

G

G

E

E

N

V

L

V

I
x
V

V

S

K

K

D

D

L

I

N

N

L

L

D

D

I

I

R

H

K

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P
|
P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

S

L

L

L

M

R

M

M

L

I

A

A

G

G

F

L

E

E

T

T

P

I

T

T

A

S

G

G

E

D

I

L

L

F

L

I

A

G

G

E

R

K

S

R

I

M

N

N

N

D

V

T

P

P

H

A

K

E

R

R

D

G

I

V

G

G

M

M

V

V

F

F

Q
|
Q

N

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

A

S

F

F

P

G

L

L

T

K

V

L

R

A

G

G

L

A

N

K

K

K

S

E

D

V

V

I

S

N

D

Q

R

R

V

V

K

N

R

Q

V

V

L

A

S

E

M

V

V

L

Q

Q

L

L

D

A

S

H

F

L

A

L

Q

D

R
|
R

Y

K

P

P

A

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

F

A

E

E

P

P

Q

S

L

V

V

F

L

L

M

L

D
|
D

E
|
E

P

P

L

L

G

S

A

N

L

L

D

D

K

A

Q

A

L

L

R

R

E

V

H

Q

M

M

Q

R

M

I

E

E

I

I

K

S

H

R

L

L

H

H

Q

K

R

R

L

L

G

G

V

R

T

T

V

M

V

I

Y

Y

V

V

T

T

H
|
H

D

D

Q

Q

G

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

V

I

A

V

V

V

F

L

H

D

Q

A

G

G

E

R

I

V

Q

A

Q

Q

I

V

A

G

P

K

P

P

R

L

T

E

L

L

Y

Y

E

H

E

Y

P

P

K

A

N

D

T

R

F

F

V

V

A

A

N

G

F

F

I

I

G

G

-

S

-

P

E

K

N

M

N

N

R

F

L

L

N

P

G

V

R

K

L

V

H

T

S

A

H

T

T

A

G

I

D

D

R

Q

C

V

L

Q

V

V

E

E

L

L

A

P

R

M

G

P

E

N

K

R

-

Q

-

V

-

W

-

L

-

P

V

V

E

E

A

S

L

R

A

D

V

V

N

Q

V

V

G

-

Q

-

P

-

G

G

E

A

P

N

V

M

T

S

L

L

S

G

I

I

R

R

P

P

E

E

R

H

V

L

S

L

L

P

N

S

G

D

V

I

S

A

E

D

Q

V

C

I

V

-

N

-

R

-

F

L

S

E

G

G

R

E

V

V

A

Q

E

V

F

V

I

E

Y

Q

L

L

G

G

D

N

H

E

V

T

R

Q

V

I

R

H

L

I

E

Q

V

I

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y24), V17 (≠ I30), P36 (= P49), S37 (= S50), G38 (= G51), C39 (≠ S52), G40 (= G53), K41 (= K54), S42 (≠ T55), T43 (= T56), Q81 (= Q94), R128 (= R141), A132 (≠ Q145), S134 (= S147), G135 (= G148), G136 (= G149), Q137 (= Q150), E158 (= E171), H191 (= H204)
binding magnesium ion: S42 (≠ T55), Q81 (= Q94), D157 (= D170)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
41% identity, 84% coverage: 15:330/374 of query aligns to 3:319/371 of 3puxA

query
sites
3puxA

V

V

S

Q

F

L

R

Q

G

N

V

V

Q

T

K

K

S

A

Y
x
W

D

-

G

G

E

E

N

V

L

V

I
x
V

V

S

K

K

D

D

L

I

N

N

L

L

D

D

I

I

R

H

K

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

S

L

L

L

M

R

M

M

L

I

A

A

G

G

F

L

E

E

T

T

P

I

T

T

A

S

G

G

E

D

I

L

L

F

L

I

A

G

G

E

R

K

S

R

I

M

N

N

N

D

V

T

P

P

H

A

K

E

R

R

D

G

I

V

G

G

M

M

V

V

F

F

Q
|
Q

N

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

A

S

F

F

P

G

L

L

T

K

V

L

R

A

G

G

L

A

N

K

K

K

S

E

D

V

V

I

S

N

D

Q

R

R

V

V

K

N

R

Q

V

V

L

A

S

E

M

V

V

L

Q

Q

L

L

D

A

S

H

F

L

A

L

Q

D

R
|
R

Y

K

P

P

A

K

Q
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

F

A

E

E

P

P

Q

S

L

V

V

F

L

L

M

L

D
|
D

E
|
E

P

P

L

L

G

S

A

N

L

L

D

D

K

A

Q

A

L

L

R

R

E

V

H

Q

M

M

Q

R

M

I

E

E

I

I

K

S

H

R

L

L

H

H

Q

K

R

R

L

L

G

G

V

R

T

T

V

M

V

I

Y

Y

V

V

T

T

H
|
H

D

D

Q

Q

G

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

V

I

A

V

V

V

F

L

H

D

Q

A

G

G

E

R

I

V

Q

A

Q

Q

I

V

A

G

P

K

P

P

R

L

T

E

L

L

Y

Y

E

H

E

Y

P

P

K

A

N

D

T

R

F

F

V

V

A

A

N

G

F

F

I

I

G

G

-

S

-

P

E

K

N

M

N

N

R

F

L

L

N

P

G

V

R

K

L

V

H

T

S

A

H

T

T

A

G

I

D

D

R

Q

C

V

L

Q

V

V

E

E

L

L

A

P

R

M

G

P

E

N

K

R

-

Q

-

V

-

W

-

L

-

P

V

V

E

E

A

S

L

R

A

D

V

V

N

Q

V

V

G

-

Q

-

P

-

G

G

E

A

P

N

V

M

T

S

L

L

S

G

I

I

R

R

P

P

E

E

R

H

V

L

S

L

L

P

N

S

G

D

V

I

S

A

E

D

Q

V

C

I

V

-

N

-

R

-

F

L

S

E

G

G

R

E

V

V

A

Q

E

V

F

V

I

E

Y

Q

L

L

G

G

D

N

H

E

V

T

R

Q

V

I

R

H

L

I

E

Q

V

I

binding adenosine-5'-diphosphate: W12 (≠ Y24), V17 (≠ I30), S37 (= S50), G38 (= G51), C39 (≠ S52), G40 (= G53), K41 (= K54), S42 (≠ T55), T43 (= T56), R128 (= R141), A132 (≠ Q145), L133 (= L146), S134 (= S147), Q137 (= Q150)
binding beryllium trifluoride ion: S37 (= S50), G38 (= G51), K41 (= K54), Q81 (= Q94), S134 (= S147), G135 (= G148), G136 (= G149), E158 (= E171), H191 (= H204)
binding magnesium ion: S42 (≠ T55), Q81 (= Q94), D157 (= D170)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
41% identity, 84% coverage: 15:330/374 of query aligns to 3:319/371 of 3puwA

query
sites
3puwA

V

V

S

Q

F

L

R

Q

G

N

V

V

Q

T

K

K

S

A

Y
x
W

D

-

G

G

E

E

N

V

L

V

I
x
V

V

S

K

K

D

D

L

I

N

N

L

L

D

D

I

I

R

H

K

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

S

L

L

L

M

R

M

M

L

I

A

A

G

G

F

L

E

E

T

T

P

I

T

T

A

S

G

G

E

D

I

L

L

F

L

I

A

G

G

E

R

K

S

R

I

M

N

N

N

D

V

T

P

P

H

A

K

E

R

R

D

G

I

V

G

G

M

M

V

V

F

F

Q
|
Q

N

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

A

S

F

F

P

G

L

L

T

K

V

L

R

A

G

G

L

A

N

K

K

K

S

E

D

V

V

I

S

N

D

Q

R

R

V

V

K

N

R

Q

V

V

L

A

S

E

M

V

V

L

Q

Q

L

L

D

A

S

H

F

L

A

L

Q

D

R
|
R

Y

K

P

P

A

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

F

A

E

E

P

P

Q

S

L

V

V

F

L

L

M

L

D

D

E
|
E

P

P

L

L

G

S

A
x
N

L

L

D

D

K

A

Q

A

L

L

R

R

E

V

H

Q

M

M

Q

R

M

I

E

E

I

I

K

S

H

R

L

L

H

H

Q

K

R

R

L

L

G

G

V

R

T

T

V

M

V

I

Y

Y

V

V

T

T

H
|
H

D

D

Q

Q

G

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

V

I

A

V

V

V

F

L

H

D

Q

A

G

G

E

R

I

V

Q

A

Q

Q

I

V

A

G

P

K

P

P

R

L

T

E

L

L

Y

Y

E

H

E

Y

P

P

K

A

N

D

T

R

F

F

V

V

A

A

N

G

F

F

I

I

G

G

-

S

-

P

E

K

N

M

N

N

R

F

L

L

N

P

G

V

R

K

L

V

H

T

S

A

H

T

T

A

G

I

D

D

R

Q

C

V

L

Q

V

V

E

E

L

L

A

P

R

M

G

P

E

N

K

R

-

Q

-

V

-

W

-

L

-

P

V

V

E

E

A

S

L

R

A

D

V

V

N

Q

V

V

G

-

Q

-

P

-

G

G

E

A

P

N

V

M

T

S

L

L

S

G

I

I

R

R

P

P

E

E

R

H

V

L

S

L

L

P

N

S

G

D

V

I

S

A

E

D

Q

V

C

I

V

-

N

-

R

-

F

L

S

E

G

G

R

E

V

V

A

Q

E

V

F

V

I

E

Y

Q

L

L

G

G

D

N

H

E

V

T

R

Q

V

I

R

H

L

I

E

Q

V

I

binding adenosine-5'-diphosphate: W12 (≠ Y24), V17 (≠ I30), S37 (= S50), G38 (= G51), C39 (≠ S52), G40 (= G53), K41 (= K54), S42 (≠ T55), T43 (= T56), R128 (= R141), A132 (≠ Q145), S134 (= S147), Q137 (= Q150)
binding tetrafluoroaluminate ion: S37 (= S50), G38 (= G51), K41 (= K54), Q81 (= Q94), S134 (= S147), G135 (= G148), G136 (= G149), E158 (= E171), N162 (≠ A175), H191 (= H204)
binding magnesium ion: S42 (≠ T55), Q81 (= Q94)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
41% identity, 84% coverage: 15:330/374 of query aligns to 4:318/369 of P19566

query
sites
P19566

V

V

S

Q

F

L

R

R

G

N

V

V

Q

T

K

K

S

A

Y

W

D

-

G

G

E

D

N

V

L

V

I

V

V

S

K

K

D

D

L

I

N

N

L

L

D

D

I

I

R

H

K

D

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

T

S

T

T

S

L

L

L

M

R

M

M

L

I

A

A

G

G

F

L

E

E

T

T

P

I

T

T

A

S

G

G

E

D

I

L

L

F

L

I

A

G

G

E

R

T

S

R

I

M

N

N

N

D

V

I

P

P

H

A

K

E

R

R

D

G

I

V

G

G

M

M

V

V

F

F

Q

Q

N

S

Y

Y

A

A

L
|
L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

A

S

F

F

P

G

L

L

T

K

V

L

R

A

G

G

L

A

N

K

K

K

S

E

D

V

V

M

S

N

D

Q

R

R

V

V

K

N

R

Q

V

V

L

A

S

E

M

V

V

L

Q

Q

L

L

D

A

S

H

F

L

A

L

Q

E

R

R

Y

K

P

P

A

K

Q

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

F

A

E

E

P

P

Q

R

L

V

V

F

L

L

M

L

D

D

E

E

P
|
P

L

L

G

S

A

N

L

L

D
|
D

K

A

Q

A

L

L

R

R

E

V

H

Q

M

M

Q

R

M

I

E

E

I

I

K

S

H

R

L

L

H

H

Q

K

R

R

L

L

G

G

V

R

T

T

V

M

V

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

G

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

V

I

A

V

V

V

F

L

H

D

Q

A

G

G

E

R

I

V

Q

A

Q

Q

I

V

A

G

P

K

P

P

R

L

T

E

L

L

Y

Y

E

H

E

Y

P

P

K

A

N

D

T

R

F

F

V

V

A

A

N

G

F

F

I

I

G

G

-

S

-

P

E

K

N

M

N

N

R

F

L

L

N

P

G

V

R

K

L

V

H

T

S

A

H

T

T

A

G

I

D

E

R

Q

C

V

L

Q

V

V

E

E

L

L

A

P

R

N

G

R

E

Q

K

Q

V

I

E

W

A

L

L

P

A

V

V

E

N

S

V

R

G

G

-

V

Q

Q

P

V

G

G

E

A

P

N

V

M

T

S

L

L

S

G

I

I

R

R

P

P

E

E

R

H

V

L

S

L

L

P

N

S

G

D

V

I

S

A

E

D

Q

V

C

T

V

-

N

-

R

-

F

L

S

E

G

G

R

E

V

V

A

Q

E

V

F

V

I
x
E

Y

Q

L

L

G

G

D

H

H

E

V

T

R

Q

V

I

R

H

L

I

E

Q

V

I

L86 (= L98) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P172) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D177) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
E306 (≠ I318) mutation to K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
41% identity, 84% coverage: 15:330/374 of query aligns to 4:320/371 of P68187

query
sites
P68187

V

V

S

Q

F

L

R

Q

G

N

V

V

Q

T

K

K

S

A

Y

W

D

-

G

G

E

E

N

V

L

V

I

V

V

S

K

K

D

D

L

I

N

N

L

L

D

D

I

I

R

H

K

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

T

S

T

T

S

L

L

L

M

R

M

M

L

I

A

A

G

G

F

L

E

E

T

T

P

I

T

T

A

S

G

G

E

D

I

L

L

F

L

I

A

G

G

E

R

K

S

R

I

M

N

N

N

D

V

T

P

P

H

A

K

E

R

R

D

G

I

V

G

G

M

M

V

V

F

F

Q

Q

N

S

Y

Y

A
|
A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

A

S

F

F

P

G

L

L

T

K

V

L

R

A

G

G

L

A

N
x
K

K

K

S

E

D

V

V

I

S

N

D

Q

R

R

V
|
V

K

N

R

Q

V
|
V

L

A

S
x
E

M

V

V

L

Q

Q

L

L

D
x
A

S

H

F

L

A

L

Q

D

R

R

Y

K

P

P

A

K

Q

A

L

L

S

S

G

G

G
|
G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

F

A

E

E

P

P

Q

S

L

V

V

F

L

L

M

L

D
|
D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

K

A

Q

A

L

L

R

R

E

V

H

Q

M

M

Q

R

M

I

E

E

I

I

K

S

H

R

L

L

H

H

Q

K

R

R

L

L

G

G

V

R

T

T

V

M

V

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

G

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

V

I

A

V

V

V

F

L

H

D

Q

A

G

G

E

R

I

V

Q

A

Q

Q

I

V

A

G

P

K

P

P

R

L

T

E

L

L

Y

Y

E

H

E

Y

P

P

K

A

N

D

T
x
R

F

F

V

V

A

A

N

G

F

F

I

I

G

G

-

S

-

P

E

K

N

M

N

N

R
x
F

L

L

N

P

G

V

R

K

L

V

H

T

S

A

H

T

T

A

G

I

D

D

R

Q

C

V

L

Q

V

V

E

E

L

L

A

P

R

M

G

P

E

N

K

R

-

Q

-

V

-
x
W

-

L

-

P

V

V

E

E

A

S

L

R

A

D

V

V

N

Q

V

V

G

-

Q

-

P

-

G
|
G

E

A

P

N

V

M

T
x
S

L

L

S
x
G

I

I

R

R

P

P

E

E

R

H

V

L

S

L

L

P

N

S

G

D

V

I

S

A

E

D

Q

V

C

I

V

-

N

-

R

-

F

L

S

E

G
|
G

R

E

V

V

A

Q

E

V

F

V

I
x
E

Y

Q

L

L

G

G

D

N

H

E

V

T

R

Q

V

I

R

H

L

I

E

Q

V

I

A85 (= A97) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ N118) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V126) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (= V129) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ S131) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ D136) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G149) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D170) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ T240) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
F241 (≠ R251) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
W267 (vs. gap) mutation to G: Normal maltose transport but constitutive mal gene expression.
G278 (= G285) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
S282 (≠ T289) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G284 (≠ S291) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G302 (= G312) mutation to D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
E308 (≠ I318) mutation to Q: Maltose transport is affected but retains ability to interact with MalT.

Sites not aligning to the query:

322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
47% identity, 76% coverage: 31:314/374 of query aligns to 22:295/353 of 1vciA

query
sites
1vciA

V

V

K

N

D

K

L

L

N

N

L

L

D

T

I

I

R

K

K

D

G

G

E

E

F

F

L

L

T

V

L

L

G

G

P
|
P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
|
T

S

T

L

L

M

R

M

M

L

I

A

A

G

G

F

L

E

E

T

E

P

P

T

T

A

E

G

G

E

R

I

I

L

Y

L

F

A

G

G

D

R

R

S

D

I

V

N

T

N

Y

V

L

P

P

H

K

K

D

R

R

D

N

I

I

G

S

M

M

V

V

F

F

Q

Q

N

-

Y

-

A

-

L

-

F

-

P

-

H

H

M

M

T

T

V

V

A

Y

E

E

N

N

L

I

A

A

F

F

P

P

L

L

T

-

V

-

R

K

G

K

L

F

N

P

K

K

S

D

D

E

V

I

S

D

D

K

R

R

V

V

K

R

R

W

V

A

L

A

S

E

M

L

V

L

Q

Q

L

I

D

E

S

E

F

L

A

L

Q

N

R

R

Y

Y

P

P

A

A

Q

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

V

A

A

R

R

A

A

L

I

V

V

F

V

E

E

P

P

Q

D

L

V

V

L

L

L

M

M

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

K

A

Q

K

L

L

R

R

E

V

H

A

M

M

Q

R

M

A

E

E

I

I

K

K

H

K

L

L

H

Q

Q

Q

R

K

L

L

G

K

V

V

T

T

V

T

V

I

Y

Y

V

V

T

T

H

H

D

D

Q

Q

G

V

E

E

A

A

L

M

T

T

M

M

S

G

D

D

R

R

V

I

A

A

V

V

F

M

H

N

Q

R

G

G

E

Q

I

L

Q

L

Q

Q

I

I

A

G

P

S

P

P

R

T

T

E

L

V

Y

Y

E

L

E

R

P

P

K

N

N

S

T

V

F

F

V

V

A

A

N

T

F

F

I

I

G

G

E

A

N

P

N

E

R

M

L

-

N

-

G

N

R

I

L

L

H

E

S

V

H

S

T

V

G

G

D

D

R

G

C

Y

L

L

V

E

E

-

L

-

A

G

R

R

G

G

E

F

K

R

V

I

E

E

A

L

L

P

A

Q

V

M

N

D

V

L

G

L

Q

K

P

D

-

Y

-

V

G

G

E

K

P

T

V

V

T

L

L

F

S

G

I

I

R

R

P

P

E

E

R

H

V

M

S

T

L

V

N

E

G

G

V

V

S

H

E

M

Q

K

C

R

V

T

N

A

R

R

F

L

S

I

G

G

R

K

V

V

binding adenosine-5'-triphosphate: P40 (= P49), S41 (= S50), G42 (= G51), C43 (≠ S52), G44 (= G53), K45 (= K54), T46 (= T55), T47 (= T56)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 16, 19

7cagC Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (see paper)
51% identity, 61% coverage: 20:247/374 of query aligns to 8:232/362 of 7cagC

query
sites
7cagC

V

V

Q

T

K

K

S

S

Y

Y

D

P

G
x
A

E

A

N

-

L

-

I

-

V

V

K

K

D

E

L

F

N

S

L

M

D

T

I

I

R

A

K

D

G

G

E

E

F

F

L

I

T

I

L

L

L

V

G

G

P
|
P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

S

T

L

L

M

N

M

M

L

I

A

A

G

G

F

L

E

E

T

E

P

I

T

T

A

S

G

G

E

E

I

L

L

R

L

I

A

G

G

G

R

E

S

R

I

V

N

N

N

E

V

K

P

A

P

P

H

K

K

D

R

R

D

D

I

I

G

A

M

M

V

V

F

F

Q
|
Q

N

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

V

V

A

R

E

Q

N

N

L

I

A

A

F

F

P

P

L

L

T

T

V

L

R

A

G

K

L

V

N

P

K

K

S

A

D

E

V

I

S

A

D

A

R

K

V

V

K

E

R

E

V

T

L

A

S

K

M

I

V

L

Q

D

L

L

D

S

S

E

F
x
L

A

L

Q

D

R
|
R

Y

K

P

P

A

G

Q
|
Q

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

V

V

F

R

E

S

P

P

Q

K

L

A

V

F

L

L

M

M

D

D

E

Q

P

P

L

L

G

S

A

N

L

L

D

D

K

A

Q

K

L

L

R

R

E

V

H

Q

M

M

Q

R

M

A

E

E

I

I

K

S

H

R

L

L

H

Q

Q

D

R

R

L

L

G

G

V

T

T

T

V

T

V

V

Y

Y

V

V

T

T

H

H

D

D

Q

Q

G

T

E

E

A

A

L

M

T

T

M

L

S

G

D

D

R

R

V

V

A

V

V

V

F

M

H

L

Q

A

G

G

E

E

I

V

Q

Q

I

I

A

G

P

T

P

P

R

D

T

E

L

L

Y

Y

E

S

P

P

K

A

N

N

T

L

F

F

V

V

A

A

N

G

F

F

I

I

G

G

binding adenosine-5'-triphosphate: A14 (≠ G26), P34 (= P49), S35 (= S50), G36 (= G51), C37 (≠ S52), G38 (= G53), K39 (= K54), S40 (≠ T55), T41 (= T56), Q79 (= Q94), L123 (≠ F138), R126 (= R141), Q130 (= Q145), S132 (= S147), G133 (= G148), G134 (= G149), Q135 (= Q150)
binding magnesium ion: S40 (≠ T55), Q79 (= Q94)

3d31A Modbc from methanosarcina acetivorans (see paper)
40% identity, 83% coverage: 23:332/374 of query aligns to 8:312/348 of 3d31A

query
sites
3d31A

S

S

Y

R

D

K

G

W

E

K

N

N

L

F

I

S

V

L

K

D

D

N

L

L

N

S

L

L

D

K

I

V

R

E

K

S

G

G

E

E

F

Y

L

F

T

V

L

I

L

L

G

G

P

P

S

T

G

G

S

A

G

G

K

K

T

T

T

L

S

F

L

L

M

E

M

L

L

I

A

A

G

G

F

F

E

H

T

V

P

P

T

D

A

S

G

G

E

R

I

I

L

L

A

D

G

G

R

K

S

D

I

V

N

T

N

D

V

L

P

S

P

P

H

E

K

K

R

H

D

D

I

I

G

A

M

F

V

V

F

Y

Q

Q

N

N

Y

Y

A

S

L

L

F

F

P

P

H

H

M

M

T

N

V

V

A

K

E

K

N

N

L

L

A

E

F

F

P

-

L

-

T

-

V

-

R

-

G

G

L

M

N

R

K

M

S

K

D

K

V

I

S

K

D

D

R

P

V

-

K

K

R

R

V

V

L

L

S

D

M

T

-

A

-

R

-

D

V

L

Q

K

L

I

D

E

S

H

F

L

A

L

Q

D

R

R

Y

N

P

P

A

L

Q

T

L

L

S

S

G

G

Q

E

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

V

V

F

T

E

N

P

P

Q

K

L

I

V

L

L

L

M

L

D

D

E

E

P

P

L

L

G

S

A

A

L

L

D

D

K

P

Q

R

L

T

R

Q

E

E

H

N

M

A

Q

R

M

E

E

M

I

L

K

S

H

V

L

L

H

H

Q

K

R

K

L

N

G

K

V

L

T

T

V

V

V

L

Y

H

V

I

T

T

H

H

D

D

Q

Q

G

T

E

E

A

A

L

R

T

I

M

M

S

A

D

D

R

R

V

I

A

A

V

V

F

V

H

M

Q

D

G

G

E

K

I

L

Q

I

Q

Q

I

V

A

G

P

K

P

P

R

E

T

E

L

I

Y

F

E

E

E

K

P

P

K

V

N

E

T

G

F

R

V

V

A

A

N

S

F

F

I

V

G

G

E

F

N

E

N

N

R

V

L

L

N

K

G

G

R

R

L

V

H

I

S

S

H

-

T

-

G

A

D

E

R

Q

C

G

L

L

V

L

E

R

L

I