SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO356_18530 FitnessBrowser__pseudo5_N2C3_1:AO356_18530 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
67% identity, 98% coverage: 1:460/471 of query aligns to 2:451/457 of P15993

query
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P15993

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Y103 (= Y103) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.

P24207 Phenylalanine-specific permease; Phenylalanine:H(+) symporter PheP from Escherichia coli (strain K12) (see 3 papers)
58% identity, 97% coverage: 5:459/471 of query aligns to 13:458/458 of P24207

query
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P24207

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R26 (= R18) mutation R->G,S,Q: Strong decrease in phenylalanine transport activity.
P54 (= P46) mutation to A: 50% of wild-type phenylalanine transport activity.; mutation to G: No change in phenylalanine transport activity.; mutation to L: 26% of wild-type phenylalanine transport activity.
F87 (= F79) mutation to L: No effect on phenylalanine transport activity.
F90 (= F82) mutation to L: 65% of wild-type phenylalanine transport activity.
Y92 (≠ H84) mutation to L: 41% of wild-type phenylalanine transport activity.
Y94 (= Y86) mutation to L: 69% of wild-type phenylalanine transport activity.
W95 (= W87) mutation to L: 10% of wild-type phenylalanine transport activity.
F98 (= F90) mutation to L: No effect on phenylalanine transport activity.
F101 (= F93) mutation to L: 38% of wild-type phenylalanine transport activity.
W105 (= W97) mutation to L: 39% of wild-type phenylalanine transport activity.
Y107 (≠ C99) mutation to L: No effect on phenylalanine transport activity.
W108 (= W100) mutation to L: 71% of wild-type phenylalanine transport activity.
F111 (≠ Y103) mutation to L: 60% of wild-type phenylalanine transport activity.; mutation to Y: Enables the transport of tryptophan to almost the same steady-state level as that of phenylalanine.
E118 (= E110) mutation E->G,L,V,N: Loss of activity.
K168 (= K160) mutation K->L,R: Strong decrease in phenylalanine transport activity.; mutation to N: Loss of activity.
E226 (= E218) mutation E->A,Q,K,R,W: Loss of activity.
R252 (= R244) mutation R->D,E,F,W,P: Loss of activity.
P341 (= P342) mutation to A: 5% of wild-type phenylalanine transport activity.; mutation P->G,Q,K,R: Loss of activity.; mutation to S: 3% of wild-type phenylalanine transport activity.; mutation to T: 17% of wild-type phenylalanine transport activity.
P442 (= P443) mutation to A: 46% of wild-type phenylalanine transport activity.; mutation to G: 52% of wild-type phenylalanine transport activity.; mutation to L: 43% of wild-type phenylalanine transport activity.

Q88CZ8 L-histidine transporter HutT from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
44% identity, 97% coverage: 11:468/471 of query aligns to 7:460/467 of Q88CZ8

query
sites
Q88CZ8

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C

G

N

A

S

G

R

R

M

M

L

M

L

Y

G

G

M

L

A

A

E

Q

Q

Q

G

G

D

H

A

A

P

P

K

R

A

G

L

F

S

S

R

K

I

L

D

S

K

K

R

H

G

G

V

V

P

P

V

W

R

M

S

T

I

V

L

V

A

V

S

M

A

G

A

A

V

A

T

L

L

L

V

I

A

G

V

V

L

L

N

N

Y

Y

L

L

V

I

P

P

Q

E

H

N

A

V

L

F

E

L

L

L

L

I

M

A

S

S

L

I

V

A

V

T

A

F

T

A

L

T

V

V

I

W

N

V

W

W

A

L

M

M

I

I

S

L

Y

L

S

T

H

Q

F

V

K

A

F

M

R

R

Q

R

H

S

M

M

N

S

Q

R

T

E

Q

Q

Q

V

T

A

P

Q

L

L

-

K

F

F

K

P

A

V

L

P

W

F

Y

W

P

P

Y

Y

G

G

N

P

Y

A

I

M

C

A

L

I

A

A

F

F

V

M

V

V

F

F

I

I

L

F

G

G

V

V

M

L

L

G

L

Y

I

F

P

P

G

D

I

T

Q

Q

I

A

S

A

V

L

Y

I

A

V

I

G

P

V

V

I

W

W

V

V

F

F

M

L

W

V

V

A

C

S

Y

Y

V

L

I

L

K

W

N

C

K

K

R

P

S

R

A

A

R

G

Q

Q

E

G

L

Q

A

P

V

V

A

A

T27 (= T31) mutation T->A,S: Retains 60% of wild-type activity.; mutation to N: Retains 20% of wild-type activity.
E98 (≠ L102) mutation to A: Retains 80% of wild-type activity.
K156 (= K160) mutation K->A,Q: Retains less than 10% of wild-type activity.; mutation to R: Retains 40% of wild-type activity.
F212 (= F212) mutation F->A,Q: Loss of activity.; mutation to Y: No change in activity.
E218 (= E218) mutation E->A,Q: Loss of activity.; mutation to D: Retains 70% of wild-type activity.

P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
35% identity, 98% coverage: 4:466/471 of query aligns to 2:456/469 of P46349

query
sites
P46349

Q

N

N

Q

S

S

H

Q

S

S

G

G

E

-

L

L

K

K

R

K

G

E

L

L

K

K

N

T

R

R

H

H

I

M

Q

T

L

M

I

I

A

S

L

I

G

A

G

G

A

V

I

I

G

G

T

A

G

G

L

L

F

F

L

V

G
|
G

S

S

A

G

G

S

V

V

L

I

K

H

S

S

A

T

G
|
G

P

P

S

G

M

A

I

V

L

V

G

S

Y

Y

A

A

I

L

C

A

G

G

F

L

I

L

A

V

F

I

M

F

I

I

M

M

R

R

Q

M

L

L

G

G

E

E

M

M

I

S

V

A

E

V

E

N

P

P

V

T

A

S

G

G

S

S

F

F

S

S

H

Q

F

Y

A

A

H

H

K

D

Y

A

W

I

G

G

G

P

F

W

A

A

G

G

F

F

L

T

S

I

G

G

W

W

N

L

C

Y

W

W

I

F

L

F

Y

W

I

V

L

I

V

V

G

I

M

A

S

I

E

E

L

A

T

I

A

A

V

G

G

A

K

G

Y

I

I

I

H

Q

Y

Y

W

W

A

F

P

H

D

D

I

I

P

P

T

L

W

W

V

L

S

T

A

S

A

L

A

I

F

L

F

T

I

I

L

V

I

L

N

T

A

L

I

T

N

N

L

V

A

Y

N

S

V

V

K

K

V

S

F

F

G

G

E

E

A

F

E

E

F

Y

W

W

F

F

A

S

I

L

I

I

K

K

V

V

A

T

I

I

V

I

G

A

M

F

I

L

A

I

L

V

G

G

-

F

S

A

Y

F

L

I

L

F

V

G

S

F

G

A

H

P

G

G

G

S

P

E

Q

P

A

V

S

G

V

F

T

S

N

N

L

L

W

T

S

G

H

K

G

G

F

F

P

P

N

E

G

G

V

I

S

S

G

S

L

V

V

L

M

L

A

G

M

I

A

V

I

V

M

I

F

F

S

S

F

F

G

M

G

G

L

T

E

E

M

I

L

V

G

A

F

I

T

A

A

A

A

G

E

E

A

T

D

S

K

N

P

P

K

I

T

E

V

S

I

V

P

T

K

K

A

A

I

T

N

R

Q

S

V

V

I

V

Y

W

R

R

I

I

L

I

I

V

F

F

Y

Y

I

V

G

G

A

S

L

I

V

A

V

I

L

V

S

A

L

L

T

L

P

P

W

W

D

N

S

S

L

-

A

-

T

-

L

-

N

-

A

-

S

-

G

-

D

A

A

N

Y

I

S

L

G

E

S

S

P

P

F

F

V

V

Q

A

V

V

F

L

S

E

M

H

L

I

G

G

S

V

N

P

T

A

A

A

H

Q

I

I

L

M

N

N

F

F

V

I

V

V

L

L

T

T

A

A

A

V

L

L

S

S

V

C

Y

L

N

N

S

S

G
|
G

T

L

Y

Y

C

T

N

T

S

S

R

R

M

M

L

L

L

Y

G

S

M

L

A

A

E

E

Q

R

G

N

D

E

A

A

P

P

K

R

A

R

L

F

S

M

R

K

I

L

D

S

K

K

R

K

G

G

V

V

P

P

V

V

R

Q

S

A

I

I

L

V

A

A

S
x
G

A

T

A

F

V
x
F

T

S

L

Y

V

I

A

A

V

V

L

V

L

M

N

N

Y

Y

L

F

V

S

P

P

Q

D

H

T

A

V

L

F

E

L

L

F

L

L

M

V

S

N

L

S

V

S

V

G

A

A

T

I

L

A

V

L

I

L

N

V

W

Y

A

L

M

V

I

I

S

A

Y

V

S

S

H

Q

F

L

K

K

F

M

R

R

Q

K

H

K

M

L

N

E

Q

K

T

T

Q

N

Q

P

T

E

P

A

L

L

F

K

K

I

A

K

L

M

W

W

-

L

Y

F

P

P

Y

F

G

L

N

T

Y

Y

I

L

C

T

L

I

A

I

F

A

V

I

V

C

F
x
G

I

I

L

L

G

V

V

S

M

M

L

A

L

F

I

I

P

D

G

S

I

M

Q

R

I

D

S

E

V

L

Y

L

A

L

I

T

P

G

V

V

W

I

V

T

V

G

F

I

M

V

W

L

V

I

C

S

Y

Y

V

L

I

V

K

F

N

R

K

K

R

R

S

K

A

V

R

S

Q

E

E

K

L

A

A

A

G33 (= G36) mutation to D: Lack of activity.
G42 (= G45) mutation to S: Lack of activity.
G301 (= G312) mutation to V: Lack of activity.
G338 (≠ S349) mutation to E: Lack of activity.
F341 (≠ V352) mutation to S: Lack of activity.
G414 (≠ F424) mutation to R: Lack of activity.

P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
34% identity, 83% coverage: 4:395/471 of query aligns to 6:398/489 of P25737

query
sites
P25737

Q

K

N

T

S

T

H

E

S

A

G

P

E

G

L

L

K

R

R

R

G

E

L

L

K

K

N

A

R

R

H

H

I

L

Q

T

L

M

I

I

A

A

L

I

G

G

A

S

I

I

G

G

T

T

G

G

L

L

F

F

L

V

G

A

S

S

A

G

G

A

V

T

L

I

K

S

S

Q

A

A

G

G

P

P

S

G

-

G

M

A

I

L

L

L

G

S

Y

Y

A

M

I

L

C

I

G

G

F

L

I

M

A

V

F

Y

M

F

I

L

M

M

R

T

Q

S

L

L

G

G

E

E

M

L

I

A

V

A

E

Y

E

M

P

P

V

V

A

S

G

G

S

S

F

F

S

A

H

T

F

Y

A

G

H

Q

K

N

Y

Y

W

V

G

E

F

G

A

F

G

G

F

F

L

A

S

L

G

G

W

W

N

N

C
x
Y

W

W

I

Y

L

N

Y
x
W

I

A

L

V

V

T

G

I

M

A

S

V

E

D

L

L

T

V

A

A

V

A

G

Q

K

L

Y

V

I

M

H

S

Y

W

W

W

A

F

P

P

D

D

I

T

P

P

T

G

W

W

V

I

S

W

A

S

A

A

A

L

F

F

F

L

I

G

L

V

I

I

N

F

A

L

I

L

N

N

L

Y

A

I

N

S

V

V

K

R

V

G

F

F

G

G

E

E

A

A

E

E

F

Y

W

W

F

F

A

S

I

L

I

I

K
|
K

V

V

V

T

A

T

I

V

V

I

G

V

M

F

I

I

A

I

L

V

G

G

S

V

Y

L

L

M

L

I

V

I

S

G

G

I

H

F

G

K

G

G

P

A

Q

Q

A

P

S

A

V

G

T

W

N

S

L

N

W

W

S

T

H

I

G

G

-

E

G

A

F

P

F

F

P

A

N

G

G

G

V

F

S

A

G

A

L

M

V

I

M

G

A

V

M

A

A

M

I

I

I

V

M

G

F
|
F

S

S

F

F

G

Q

G

G

L

T

E
|
E

M

L

L

I

G

G

F

I

T

A

A

A

A

G

E
|
E

A

S

D

E

K

D

P

P

K

A

T

K

V

N

I

I

P

P

K

R

A

A

I

V

N

R

Q

Q

V

V

I

F

Y

W

R

R

I

I

L

L

I

L

F

F

Y

Y

I

V

G

F

A

A

L

I

V

L

V

I

L

I

L

S

S

L

L

I

T

I

P

P

W

Y

D

T

S

D

L

P

L

S

A

L

T

L

L

R

N

N

A
x
D

S

V

G

K

D
|
D

A

I

Y

-

S

S

G

V

S

S

P

P

F

F

V

T

Q

L

V

V

F

F

S

Q

M

H

L

A

G

G

S

L

N

L

T

S

A

A

H

A

I

V

L

M

N

N

F

A

V

V

V

I

L

L

T

T

A

A

A

V

L

L

S

S

V

A

Y

G

N

N

S

S

G

G

T

M

Y

Y

C

A

N

S

S

T

R

R

M

M

L

L

L

Y

G

T

M

L

A

A

E

C

Q

D

G

G

D

K

A

A

P

P

K

R

A

I

L

F

S

A

R

K

I

L

D

S

K

R

R

G

G

G

V

V

P

P

V

R

R

N

S

A

I

L

L

Y

A

A

S

T

A

T

A

V

V

I

T

A

L

G

V

L

A

C

V

F

L

L

L

T

N

S

Y

M

L

F

V

G

P

N

Q

Q

H

T

A

V

L

Y

E

L

L

W

L

L

M

L

S

N

L

T

V

S

V

G

A

M

T

T

L

G

V

F

I

I

N

A

W

W

A

L

M

G

I

I

S

A

Y

I

S

S

H

H

F

Y

K

R

F

F

R

R

Q

R

Y102 (≠ C99) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
W106 (≠ Y103) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
K163 (= K160) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
F216 (= F212) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
E222 (= E218) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
E230 (= E226) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
D275 (≠ A271) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
D278 (= D274) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
438 E→A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
443 D→A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
446 D→A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.

P04817 Arginine permease CAN1; Canavanine resistance protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
34% identity, 89% coverage: 7:426/471 of query aligns to 80:510/590 of P04817

query
sites
P04817

H

Q

S

N

G

A

E

E

L

V

K

K

R

R

G

E

L

L

K

K

N

Q

R

R

H

H

I

I

Q

G

L

M

I

I

A

A

L

L

G

G

A

T

I

I

G

G

T

T

G

G

L

L

F

F

L

I

G

G

S

L

A

S

G

T

V
x
P

L

L

K

T

S

N

A

A

G

G

P

P

-

V

S

G

M

A

I

L

L

I

G

S

Y

Y

A

L

I

F

C

M

G

G

F

S

I

L

A

A

F

Y

M

S

I

V

M

T

R

Q

Q

S

L

L

G

G

E

E

M

M

I

A

V

T

E

F

E

I

P
|
P

V
|
V

A

T

G

S

S
|
S

F

F

S

T

H

V

F

F

A

S

H

Q

K

R

Y

F

W

L

G

S

G

P

F

A

A

F

G

G

F

A

L

A

S

N

G

G

W

Y

N

M

C
x
Y

W

W

I

F

L

S

Y

W

I

A

L

I

V

T

G

F

M

A

S

L

E

E

L

L

T

S

A

V

V

V

G

G

K

Q

Y

V

I

I

H

Q

Y

F

W

W

A

T

P

Y

D

K

I

V

P

P

-

L

-

A

T

A

W

W

V

I

S

S

A

I

A

-

F

F

F

W

I

V

L

I

I

I

N

T

A

I

I

M

N

N

L

L

A

F

N

P

V

V

K

K

V

Y

F

Y

G

G

E

E

A

F

E

E

F

F

W

W

F

V

A

A

I

S

I

I

K

K

V

V

V

L

A

A

I

I

V

I

G

G

M

F

I

L

A

-

L

I

G

Y

S

C

Y

F

L

C

L

M

V

V

S

C

G

G

H

A

G

G

G

V

P

T

Q

G

A

P

S

V

V

G

T

F

N

R

L

Y

W

W

S

R

H

N

G

P

G

G

F

A

F

W

P

G

N

P

G

G

V

I

S

I

-

S

-

K

-

D

-

K

-

N

-

E

-

G

-

R

-

F

-

L

G

G

L

W

V

V

M

S

A

S

M

L

A

I

I

N

I

A

M

A

F

F

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T

F

F

G

Q

G

G

L

T

E

E

M

L

L

V

G

G

F

I

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T

A

A

A
x
G

E

E

A

A

D

A

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N

P
|
P

K

R

T

K

V

S

I

V

P

P

K

R

A

A

I

I

N

K

Q

K

V

V

I

V

Y

F

R

R

I

I

L

L

I

T

F

F

Y

Y

I

I

G

G

A

S

L

L

V

L

V

F

L

I

L

G

S

L

L

L

T

V

P

P

W

Y

D

N

S

D

L

-

A

P

T

K

L

L

N

T

A

Q

S

S

G
x
T

D
x
S

A
x
Y

Y

V

S

S

G

T

S

S

P

P

F

F

V

I

Q

I

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A

F

I

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M

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G

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N

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H

H

I

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N

N

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V

V

I

L

L

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T

A

T

A

I

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Y

A

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N

S

S

G

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I

Y

Y

C

V

N

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S

S

R

R

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L

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G

M

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P

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P

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A

A

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L

A

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L

A

A

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M

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E

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T

Y

S

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T

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P

G

Q

D

H

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A

V

L

F

E

E

L
x
W

L

L

M

L

S

N

L

I

V

T

V

G

A

V

T

A

L

G

V
x
F

I

F

N

A

W

W

A

L

M

F

I

I

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S

Y

I

S

S

H

H

F

I

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F

F

R

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Q

Q

H

A

M

L

N

K

-

Y

-

R

-

G

-

I

Q

S

T

R

Q

D

Q

E

T

L

P

P

L

-

F

F

K

K

A

A

L

K

W

L

Y

M

P

P

Y

G

G

L

N

A

Y

Y

I

Y

C

A

L

A

A

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F

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M

V

T

F

I

I

I

L

I

P113 (≠ V40) mutation to L: In CAN1-343; confers citrulline transport activity in GAP1-deleted cells.
P148 (= P74) mutation to L: In CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine.
V149 (= V75) mutation to F: In CAN1-315; confers citrulline transport activity in GAP1-deleted cells.
S152 (= S78) mutation to F: In CAN1-342; confers citrulline transport activity in GAP1-deleted cells.
Y173 (≠ C99) mutation to D: In CAN1-306; confers citrulline transport activity in GAP1-deleted cells.; mutation to H: In CAN1-327; confers citrulline transport activity in GAP1-deleted cells.
G308 (≠ A225) mutation to A: In CAN1-341; confers citrulline transport activity in GAP1-deleted cells.
P313 (= P230) mutation to S: In CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine.
TS 354:355 (≠ GD 273:274) mutation Missing: In CAN1-318; confers citrulline transport activity in GAP1-deleted cells.
Y356 (≠ A275) mutation to H: In CAN1-340; confers citrulline transport activity in GAP1-deleted cells.; mutation to N: In CAN1-339; confers citrulline transport activity in GAP1-deleted cells.
W451 (≠ L370) mutation to C: In CAN1-328; confers citrulline transport activity in GAP1-deleted cells.; mutation to L: In CAN1-316; confers citrulline transport activity in GAP1-deleted cells.; mutation to S: In CAN1-335; confers citrulline transport activity in GAP1-deleted cells.
F461 (≠ V380) mutation to S: In CAN1-307; confers citrulline transport activity in GAP1-deleted cells.

P48813 High-affinity glutamine permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
31% identity, 84% coverage: 4:398/471 of query aligns to 138:538/663 of P48813

query
sites
P48813

Q

Q

N

E

S

Q

H

K

S

Q

G

E

E

N

L

L

K

K

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K

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A

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I

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F

L

L

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A

S

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V

L

L

K

N

S

N

A

A

G

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P

P

S

G

-

G

M

L

I

I

L

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Y

A

A

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G

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E

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-

A

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D

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A

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A

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F

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V

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D

G

P

F

A

A

L

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F

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G

A

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C

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A

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A

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Y

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A

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F

A

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A

I

F

F

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Y

I

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L

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I

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N

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A

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A

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F

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-

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F

A

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A

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A

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H

H

F

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F

F

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Q

R

H

A

M

M

N

K

Sites not aligning to the query:

132 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

P19145 General amino-acid permease GAP1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
33% identity, 82% coverage: 11:397/471 of query aligns to 86:478/602 of P19145

query
sites
P19145

L

L

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K

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G

H

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L

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-

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-

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A

A

A

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A

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A

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A

F

F

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A

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D

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T

-

P

-

G

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A

-

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-

F

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K

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A

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M

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A

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I

A

M

A

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F

G
x
A

G

G

L

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M

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A

A

A

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E

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P

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K

A

A

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A

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Q

V

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Y

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R

R

I

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I

L

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F

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Y

I

I

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L

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S

L

L

V

L

V

M

L

I

L

G

S

L

L

L

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P

P

W

Y

D

N

S

D

L

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L

S

A

L

T

I

L

G

N

A

A

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S

S

G

V

D

D

A

A

Y

-

S

A

G

A

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S

P

P

F

F

V

V

Q

I

V

A

F

I

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G

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K

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G

A

L

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P

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S

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L

V

N

N

F

V

V

V

V

I

L

L

T

I

A

A

A

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L

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S

V

V

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G

N

N

S

S

G

A

T

I

Y

Y

C

A

N

C

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S

R

R

M

T

L

M

L

V

G

A

M

L

A

A

E

E

Q

Q

G

R

D

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A

L

P

P

K

E

A

I

L

F

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S

R

Y

I

V

D

D

K

R

R

K

G

G

V

R

P

P

V

L

R

V

S

G

I

I

L

A

A

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A

S

A

A

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T

G

L

L

V

I

A

A

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F

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V

L

A

N

A

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K

P

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H

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A

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A

L

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A

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T

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W

A

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G

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F

R

R

Q

K

H

A

M

L

A297 (≠ G215) mutation to V: Impairs basic amino-acids transport and regulation by these amino-acids.

Sites not aligning to the query:

76 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q9URZ4 Cationic amino acid transporter 1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
36% identity, 72% coverage: 11:347/471 of query aligns to 80:414/587 of Q9URZ4

query
sites
Q9URZ4

L

L

K

K

R

R

G

S

L

L

K

K

N

S

R

R

H

H

I

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Q

Q

L

M

I

I

A

S

L

I

G

G

A

A

I

I

G

G

T

T

G

G

L

L

F

Y

L

V

G

G

S

S

A

G

G

S

V

S

L

L

K

A

S

D

A

G

G

G

P

P

-

A

S

S

M

V

I

I

L

I

G

N

Y

Y

A

S

I

L

C

I

G

G

F

I

I

M

A

M

F

F

M

F

I

I

M

V

R

Y

Q

A

L

L

G

G

E

E

M

M

I

A

V

V

E

A

E

Y

P

P

V

V

A

A

G

G

S

G

F

F

S

N

H

T

F

Y

A

A

H

T

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R

Y

F

W

I

G

D

G

P

F

A

A

W

G

G

F

F

L

A

S

V

G

S

W

W

N

N

C

Y

W

F

I

I

L

N

Y

Y

I

F

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G

F

M

P

S

L

E

E

L

L

T

T

A

T

V

C

G

A

K

I

Y

T

I

F

H

R

Y

Y

W

W

A

T

P

-

D

D

I

I

P

N

T

S

W

A

V

A

S

W

A

I

A

S

A

I

F

F

F

L

I

V

L

V

I

I

N

I

A

I

I

V

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N

L

L

A

F

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G

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V

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R

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A

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G

G

E

E

A

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E

E

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I

F

L

A

S

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I

V

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K

V

V

A

A

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G

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F

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I

A

I

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L

G

A

S

-

Y

-

L

I

V

I

S

N

G

C

H

G

G

G

G

V

P

P

Q

T

A

-

S

-

V

-

T

-

N

-

L

-

W

-

S

D

H

H

G

R

G

G

F

Y

-

I

-

G

-

S

-

I

-

K

-

H

-

K

-

P

F

F

P

R

N

H

G

G

V

F

S

K

G

G

L

F

V

C

M

S

A

V

M

F

A

T

I

T

I

A

M

A

F

F

S

S

F

F

G

S

G

G

L

T

E

E

M

V

L

I

G

G

F

L

T

A

A

A

E

E

A

V

D

D

K

N

P

P

K

Q

T

K

V

A

I

L

P

P

K

H

A

A

I

V

N

K

Q

Q

V

V

I

F

Y

W

R

R

I

I

L

A

I

I

F

F

Y

Y

I

V

G

V

A

S

L

L

V

I

V

L

L

I

-

G

L

L

S

L

L

I

T

S

P

P

W

D

D

D

S

-

L

-

A

P

T

N

L

L

N

M

A

G

S

N

G

G

D

S

A

T

Y

-

S

S

G

V

S

S

P

P

F

F

V

V

Q

L

V

A

F

I

S

K

M

E

L

A

G

N

S

I

N

K

T

G

A

L

A

P

H

S

I

V

L

F

N

N

F

A

V

V

V

I

L

I

T

I

A

S

A

V

L

V

S

S

V

V

Y

T

N

N

S

S

G

S

T

T

Y

Y

C

T

N

A

S

G

R

R

M

T

L

L

L

H

G

G

M

M

A

A

E

N

Q

L

G

K

D

Q

A

A

P

P

K

S

A

F

L

F

S

K

R

Y

I

T

D

D

K

R

R

L

G

G

V

R

P

P

V

L

R

L

S

A

I

M

L

I

Sites not aligning to the query:

29 modified: Phosphoserine
30 modified: Phosphoserine
37 modified: Phosphoserine

Q03770 SPS-sensor component SSY1; Amino-acid permease homolog SSY1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
27% identity, 88% coverage: 11:425/471 of query aligns to 277:770/852 of Q03770

query
sites
Q03770

L

I

K

Q

R

R

G

K

L

L

K

K

N

V

R

R

H

H

I

I

Q

Q

L

M

I

L

A

S

L

I

G

G

G

A

A

C

I

F

G

S

T

V

G

G

L

L

F

F

L

L

G

T

S

S

A

G

G

K

V

A

L

F

K

S

S

I

A

A

G

G

P

P

-

F

S

G

M

T

I

L

L

L

G

G

Y

F

A

G

I

L

C

T

G

G

F

S

I

I

A

I

F

L

M

A

I

T

M

M

R

L

Q

S

L

F

G

T

E

E

M

L

I

S

V

T

E

L

E

I

P

P

V

V

A

S

G

S

S

G

F

F

S

S

H

G

F

L

A

A

H

S

K

R

Y

F

W

V

G

E

G

D

F

A

A

F

G

G

F

F

L

A

S

L

G

G

W

W

N

T

C

Y

W

W

I

I

L

S

Y

C

I

M

L

L

V

A

G

L

M

P

S

A

E

Q

L

V

T

S

A

S

V

S

G
x
T

K

F

Y

Y

I

L

H

S

Y

Y

W

Y

A

N

P

N

-

V

D

N

I

I

P

S

T

K

W

G

V

V

S

T

A

-

A

A

A

G

F

F

F

I

I

T

L

L

I

F

N

S

A

A

-

F

-

S

-

I

-

V

I

V

N

N

L

L

A

L

N

D

V

V

K

S

V

I

F

M

G

G

E

E

A

I

E

V

F

Y

W

V

F

A

A

G

I

I

I

S

K

K

V

V

V

I

A

I

I

A

V

I

G

L

M

M

I

V

A

-

L

F

G

T

S

M

Y

I

L

I

L

L

V

N

S

A

G

G

H

H

G

G

G

N

P

D

-

I

-

H

-

E

-

G

-

V

-

G

-

F

-

R

-

Y

-

W

-

D

-

S

Q

S

A

K

S

S

V

V

T

R

N

N

L

L

W

-

S

T

H

Y

G

G

G

L

F

Y

F

R

P

P

-

T

-

F

-

D

-

L

-

A

-

D

-

A

-

G

-

E

-

G

-

S

-

K

-

G

-

I

N

S

G

G

V

P

S

K

G

G

L

R

V

F

M

L

A

A

M

T

A

A

I

S

I

V

M

M

-

L

-

I

-

S

-

T

F

F

S

A

F

F

G

S

G

G

L

V

E

E

M

M

L

T

G

F

F

L

T

A

A

S

A

G

E

E

A

A

D

I

K

N

P

P

K

R

T

K

V

T

I

I

P

P

K

S

A

A

I

T

N

K

Q

R

-

T

-

F

-

S

-

I

-

V

-

L

V

I

I

S

Y

Y

R

V

I

F

L

L

I

I

F

F

Y

S

I

V

G

G

A

I

L

N

V

I

V

Y

-

S

-

G

-

D

-

P

-

R

L

L

S

S

L

Y

T

F

P

P

-

G

-

I

-

S

-

E

-

K

-

R

-

Y

-

E

-

A

-

I

-

K

-

G

-

T

-

G

W

M

D

D

S

W

L

R

L

L

A

R

T

T

L

N

N

C

A

R

S

G

G

G

D

I

A

D

Y

Y

S

R

-

Q

-

I

-

S

-

V

-

G

-

T

-

G

-

Y

G

S

S

S

P

P

F

W

V

V

Q

V

V

A

F

L

S

Q

M

N

L

F

G

G

S

L

N

C

T

T

A

F

A

A

H

S

I

A

L

F

N

N

F

A

V

I

V

L

L

I

T

F

A

F

A

T

L

A

S

T

V

A

Y

G

N

I

S

S

G

S

T

L

Y

F

C

S

N

C

S

S

R

R

M

T

L

L

L

Y

G

A

M

M

A

S

E

V

Q

Q

G

R

D

K

A

A

P

P

K

P

A

V

L

F

S

E

R

I

I

C

D

S

K

K

R

R

G

G

V

V

P

P

V

Y

R

V

S

S

I

V

L

I

A

F

S

S

A

S

A

L

V

F

T

S

L

V

V

I

A

A

V

Y

L

I

L

-

N

-

Y

-

L

A

V

V

P

D

Q

Q

H

T

A

A

L

I

E

E

-

N

-

F

-

D

L

V

L

L

M

A

S

N

L

V

V

S

A

A

T

S

L

T

V

S

I

I

N

I

W

W

A

M

M

G

I

L

S

N

Y

L

S

S

H

F

F

L

K

R

F

F

R

Y

Q

Y

H

A

M

L

N

K

Q

Q

T

R

Q

K

Q

D

T

I

P

I

L

S

F

R

K

N

A

D

L

S

W

S

Y

Y

P

P

Y

Y

G

K

N

S

-

P

-

F

-

Q

-

P

Y

Y

I

L

-

A

-

I

-

Y

-

G

-

L

-

V

-

G

C

C

L

S

A

L

F

F

V

V

V

I

F

F

I

M

T382 (≠ G115) mutation T->H,L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids.; mutation to K: In SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids.; mutation to R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids.

P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
27% identity, 37% coverage: 67:238/471 of query aligns to 74:242/461 of P76037

query
sites
P76037

G

G

E

K

M

L

I

V

V

R

E

Q

E

F

P

P

V

E

A

A

G

G

S

S

F

A

S

Y

H

T

F

Y

A

A

H

Q

K

K

Y

S

W

I

G

N

G

P

F

H

A

V

G

G

F

F

L

M

S

V

G

G

W

W

N

S

C

S

W

L

I

L

L

D

Y
|
Y

I

L

L

F

V

L

G

P

M

M

S

I

E

N

L

V

T

L

A

L

V

A

G

K

K

I

Y

Y

I

L

H

S

Y

A

W

L

A

F

P

P

D

E

I

V

P

P

T

P

W

W

V

V

S

W

A

V

A

T

F

F

F

V

I

A

L

I

I

L

N

T

A

A

I

A

N

N

L

L

A

K

N

S

V

V

K

N

V

L

F

V

G

A

E

N

A

F

E

N

F

T

W

L

F

F

A

V

I

L

I

V

K

Q

V

I

V

S

A

I

I

M

V

V

G

V

M

F

I

I

A

-

L

-

G

-

S

-

Y

F

L

L

L

V

V

V

S

Q

G

G

-

L

H

H

G

K

G

G

P

-

Q

-

A

E

S

G

V

V

T

G

N

T

L

V

W

W

S

S

H

L

G

Q

G

P

F

F

F

I

P

S

N

E

G

N

V

A

S

H

-

L

-

I

G

P

L

I

V

I

M

T

A

G

M

A

A

T

I

I

I

V

M

C

F

F

S

S

F

F

G

L

G

G

L

F

E

D

M

A

L

V

G

T

F

T

T

L

A

S

A

E

E

E

A

T

D

P

K

D

P

A

K

A

T

R

V

V

I

I

P

P

K

K

A

A

I

I

Y110 (= Y103) mutation to X: The uptake activity is reduced to one-eighth of that of wild-type.

P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
22% identity, 79% coverage: 5:374/471 of query aligns to 22:414/629 of P30825

query
sites
P30825

N

S

S

R

H

E

S

E

G

T

E

R

L

L

K

S

R

R

G

C

L

L

K

N

N

T

R

F

H

D

I

L

Q

V

L

A

I

L

A

G

L

V

G

G

G

S

A

T

I

L

G

G

T

A

G

G

L

V

F

Y

-

V

L

L

G

A

S

G

A

A

G

V

V

A

L

R

K

E

S

N

A

A

G

G

P

P

S

A

M

I

I

V

L

I

G

S

Y

F

A

L

I

I

C

A

G

A

F

L

I

A

A

S

F

V

M

L

I

A

M

G

R

L

Q

C

L

Y

G

G

E

E

M

F

I

G

V

A

E

R

E

V

P

P

V

K

A

T

G

G

S

S

F

A

S

Y

H

L

F

Y

A

S

H

Y

K

V

Y

T

W

V

G

G

G

E

F

L

A

W

G

A

F

F

L

I

S

T

G

G

W

W

N

N

C

L

W

I

I

L

L

S

Y

Y

I

I

L

-

V

I

G

G

M

T

S

S

E

S

L

V

T

A

A

-

V

-

G

-

K

-

Y

-

I

-

H

R

Y

A

W

W

A

S

P

A

D

T

I

F

P

D

T

E

W

L

V

I

S

G

A

R

A

P

A

I

F

G

F

E

I

F

L

S

I

R

N

T

A

H

I

M

N

T

L

L

A

N

N

A

V

P

K

G

V

V

F

L

G

A

E

E

A

N

E

P

F

D

W

I

F

F

A

A

I

V

I

I

-

I

-

L

-

I

-

L

-

T

-

G

-

L

-

T

-

L

-

G

-

V

-

K

-

E

-

S

-

A

-

M

-

V

-

N

K

K

V

I

V

F

A

T

I

C

V

I

G

N

M

V

I

L

A

V

L

L

G

G

S

-

Y

F

L

I

L

M

V

V

S

S

G

G

H

F

G

-

P

V

Q

K

A

G

S

S

V

V

T

K

N

N

L

-

W

W

S

Q

H

L

-

T

-

E

-

D

-

F

-

G

-
x
N

-

T

-

S

-

G

-

R

-

L

-

C

-

L

-

N

-

D

-

T

-

K

-

E

-

G

-

K

-

P

-

G

-

V

G

G

F

F

F

M

P

P

N

F

G

G

V

F

S

S

G

G

L

V

V

L

M

S

A

G

M

A

A

A

I

T

I

C

M

F

F

Y

S

A

F

F

G

V

G

G

L

F

E

D

M

C

L

I

G

A

F

T

T

T

A

G

A

E

E

E

A

V

D

K

K

N

P

P

K

Q

T

K

V

A

I

I

P

P

K

V

A

G

I

I

N

V

Q

A

V

S

I

L

Y

L

R

I

I

C

L

F

I

I

F

A

Y

Y

I

F

G

G

A

V

L

S

V

A

L

L

L

T

S

L

L

M

T

M

P

P

W

Y

D

F

S

C

L

L

L

-

A

-

T

-

L

-

N

-

A

-

S

-

G

-

D

-

A

-

Y

D

S

N

G

N

S

S

P

P

F

L

V

P

Q

D

V

A

F

F

S

K

M

H

L

V

G

G

S

W

N

E

T

G

A

A

A

K

H

Y

I

A

L

V

N

A

F

V

V

G

V

S

L

L

T

C

A

A

A

L

L

S

S

A

V

S

Y

L

N

L

S

G

G

S

T

M

Y

F

C

P

N

M

S

P

R

R

M

V

L

I

L

Y

G

A

M

M

A

A

E

E

Q

D

G

G

D

L

A

L

P

F

K

K

A

F

L

L

S

A

R

N

I

V

-

N

D

D

K

R

R

T

G

K

V

T

P

P

V

I

R

I

S

A

I

T

L

L

A

A

S

S

A

G

A

A

V

-

T

-

L

-

V

V

A

A

V

A

L

V

L

M

N

A

Y

F

L

L

V

F

P

D

Q

L

H

K

A

D

L

L

E

V

L

D

L

L

M

M

S

S

L

I

N226 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine

8xyjA Structure of y+lat1 bound with lys
23% identity, 88% coverage: 10:423/471 of query aligns to 4:404/459 of 8xyjA

query
sites
8xyjA

E

K

L

L

K

K

R

K

G

E

L

I

K

S

N

L

R

L

H

N

I

G

Q

V

L

C

I

L

A

I

L

V

G

G

G
x
N

A
x
M

I

I

G
|
G

T

S

G

G

L

I

F

F

L

V

G

S

S

P

A

K

G

G

V

V

L

L

-

I

-

Y

-

S

K

A

S

S

A

F

G

G

P

L

S

S

M

L

I

V

L

I

G

-

Y

W

A

A

I

V

C

G

G

G

F

L

I

F

A

S

F

V

-

F

-

G

-

A

M

L

I

C

M

Y

R

A

Q

E

L

L

G

G

E

T

M

T

I

I

V

K

E

K

E

-

P

-

V

-

A

S

G

G

S

A

F

S

S

Y

H

A

F

Y

A

I

H

L

K

E

Y

A

W

F

G

G

F

F

A

L

G

A

F

F

L

I

S

R

G

L

W

W

N

T

C

S

W

-

I

-

L

L

Y

L

I

I

L

I

V

E

G

P

M

T

S

S

E

Q

L

A

T

I

A

I

V

A

G

I

K

T

Y

F

I

A

H

N

Y

Y

-

M

-

V

-

Q

-

P

-

L

-

F

-

P

-

S

-

C

W

F

A

A

P

P

D

Y

I

A

P

A

T

S

W

R

V

L

S

L

A

A

F

C

F

I

I

C

L

L

I

L

N

T

A

F

I

I

N

N

L

C

A

A

N

Y

V

V

K

K

V

W

F

G

G

T

E

L

A

V

E

Q

F

D

W

I

F

F

A

T

I

Y

I

A

K

K

V

V

V

L

A

A

I

L

V

I

G

A

M

V

I

I

A

V

L

A

G

G

S

-

Y

-

L

-

L

I

V

V

S

R

G

L

H

G

G

Q

G

G

P

A

Q

S

A

T

S

H

V

F

T

E

N

N

L

S

W

F

S

E

H

G

G

S

F

F

F

-

P

-

N

-

G

A

V

V

S

G

G

D

L

I

V

A

M

L

A

A

M

L

A

Y

I

S

I

A

M

L

F

F

S

S

F

Y

G
x
S

G
|
G

L

W

E

D

M

T

L

L

G

N

F

Y

T

V

A

T

A

E

E

E

A

I

D

K

K

N

P

P

K

E

T

R

V

N

I

L

P

P

K

L

A

S

I

I

N

G

Q

I

V

S

I

M

Y

P

R

I

I

V

L

T

I

I

F

I

Y

Y

I

I

G

L

A

T

L

N

V

V

V

A

L

Y

S

T

L

V

T

L

P

D

W

M

D

R

S

D

L

I

L

L

A

A

T

S

L

-

N

-

A

-

S

-

G

-

D

D

A

A

Y

V

S

A

G

V

S

T

P

F

F

A

V

D

Q

Q

V

I

F

F

S

G

M

-

L

-

G

-

S

-

N

-

T

-

A

-

H

-

I

I

L

F

N

N

F

W

V

I

-

P

L

L

T

S

A

V

A

A

L

L

S

S

V

C

Y

F

-

G

-

L

N

N

S

A

G

S

T

I

Y

V

C

A

N

A

S

S

R

R

M

L

L

F

G

V

M

G

A

S

E

R

Q

E

G

G

D

H

A

L

P

P

K

D

A

A

L

I

S

C

R

M

I

I

D

H

-

V

K

E

R

R

G

F

V

T

P

P

V

V

R

P

S

S

I

L

L

L

A

F

S

N

A

G

A

I

V

M

T

A

L

L

V

I

A

-

V

-

L

-

N

-

Y

Y

L

L

V

C

P

V

Q

E

H

D

A

I

L

F

E

Q

L

L

L

-

M

-

S

-

L

-

V

-

A

-

T

-

L

-

V

-

I

I

N

N

W

Y

A

Y

M

S

I

F

S

S

Y

Y

S

W

H

F

F

F

K

V

-

G

-

L

-

S

-

I

-

V

-

G

-

Q

-

L

F

Y

R

L

Q

R

H

W

M

K

N

E

Q

P

T

D

Q

R

Q

P

T

R

P

P

L

L

F

K

K

L

A

S

L

V

W

F

Y

F

P

P

Y

I

G

V

N

F

Y

C

I

L

C

C

L

T

A

I

F

F

V

L

V

V

binding lysine: N21 (≠ G27), M22 (≠ A28), G24 (= G30), S212 (≠ G215), G213 (= G216)

8xxiA Structure of y+lat1 bound with leu
23% identity, 88% coverage: 10:423/471 of query aligns to 4:404/465 of 8xxiA

query
sites
8xxiA

E

K

L

L

K

K

R

K

G

E

L

I

K

S

N

L

R

L

H

N

I

G

Q

V

L

C

I

L

A

I

L

V

G

G

G
x
N

A
x
M

I
|
I

G
|
G

T
x
S

G
|
G

L

I

F

F

L

V

G

S

S

P

A

K

G

G

V

V

L

L

-

I

-

Y

-

S

K

A

S

S

A

F

G

G

P

L

S

S

M

L

I

V

L

I

G

-

Y

W

A

A

I

V

C

G

G

G

F

L

I

F

A

S

F

V

-

F

-

G

-

A

M

L

I

C

M

Y

R

A

Q

E

L

L

G

G

E

T

M

T

I

I

V

K

E

K

E

-

P

-

V

-

A

S

G

G

S

A

F

S

S

Y

H

A

F

Y

A

I

H

L

K

E

Y

A

W

F

G

G

F

F

A

L

G

A

F

F

L

I

S

R

G

L

W

W

N

T

C

S

W

-

I

-

L

L

Y

L

I

I

L

I

V

E

G

P

M

T

S

S

E

Q

L

A

T

I

A

I

V

A

G

I

K

T

Y

F

I

A

H

N

Y

Y

-

M

-

V

-

Q

-

P

-

L

-

F

-

P

-

S

-

C

W

F

A

A

P

P

D

Y

I

A

P

A

T

S

W

R

V

L

S

L

A

A

F

C

F

I

I

C

L

L

I

L

N

T

A

F

I

I

N

N

L

C

A

A

N

Y

V

V

K

K

V

W

F

G

G

T

E

L

A

V

E

Q

F

D

W

I

F

F

A

T

I

Y

I

A

K

K

V

V

V

L

A

A

I

L

V

I

G

A

M

V

I

I

A

V

L

A

G

G

S

-

Y

-

L

-

L

I

V

V

S

R

G

L

H

G

G

Q

G

G

P

A

Q

S

A

T

S

H

V

F

T

E

N

N

L

S

W

F

S

E

H

G

G

S

F

F

F

-

P

-

N

-

G

A

V

V

S

G

G

D

L

I

V

A

M

L

A

A

M

L

A

Y

I

S

I

A

M

L

F

F

S
|
S

F

Y

G
x
S

G
|
G

L

W

E

D

M

T

L

L

G

N

F

Y

T

V

A

T

A

E

E

E

A

I

D

K

K

N

P

P

K

E

T

R

V

N

I

L

P

P

K

L

A

S

I

I

N

G

Q

I

V

S

I

M

Y

P

R

I

I

V

L

T

I

I

F

I

Y

Y

I

I

G

L

A

T

L

N

V

V

V

A

L

Y

S

T

L

V

T

L

P

D

W

M

D

R

S

D

L

I

L

L

A

A

T

S

L

-

N

-

A

-

S

-

G

-

D

D

A

A

Y

V

S

A

G

V

S

T

P

F

F

A

V

D

Q

Q

V

I

F

F

S

G

M

-

L

-

G

-

S

-

N

-

T

-

A

-

H

-

I

I

L

F

N

N

F

W

V

I

-

P

L

L

T

S

A

V

A

A

L

L

S

S

V

C

Y

F

-

G

-

L

N

N

S

A

G

S

T

I

Y

V

C

A

N

A

S

S

R

R

M

L

L

F

G

V

M

G

A

S

E

R

Q

E

G

G

D

H

A

L

P

P

K

D

A

A

L

I

S

C

R

M

I

I

D

H

-

V

K

E

R

R

G

F

V

T

P

P

V

V

R

P

S

S

I

L

L

L

A

F

S

N

A

G

A

I

V

M

T

A

L

L

V

I

A

-

V

-

L

-

N

-

Y

Y

L

L

V

C

P

V

Q

E

H

D

A

I

L

F

E

Q

L

L

L

-

M

-

S

-

L

-

V

-

A

-

T

-

L

-

V

-

I

I

N

N

W

Y

A

Y

M

S

I

F

S

S

Y

Y

S

W

H

F

F

F

K

V

-

G

-

L

-

S

-

I

-

V

-

G

-

Q

-

L

F

Y

R

L

Q

R

H

W

M

K

N

E

Q

P

T

D

Q

R

Q

P

T

R

P

P

L

L

F

K

K

L

A

S

L

V

W

F

Y

F

P

P

Y

I

G

V

N

F

Y

C

I

L

C

C

L

T

A

I

F

F

V

L

V

V

binding leucine: N21 (≠ G27), M22 (≠ A28), I23 (= I29), G24 (= G30), S25 (≠ T31), G26 (= G32), S210 (= S213), S212 (≠ G215), G213 (= G216)

7cmiB The lat2-4f2hc complex in complex with leucine (see paper)
24% identity, 71% coverage: 23:355/471 of query aligns to 8:337/458 of 7cmiB

query
sites
7cmiB

I

I

A

I

L

V

G

G

G

N

A
x
I

I

I

G
|
G

T

S

G
|
G

L

I

F

F

L

V

G

S

S

P

A

K

G

G

V

V

L

L

K

E

S

N

A

A

G

G

P

S

S

V

-

G

-

L

M

A

I

L

L

I

G

V

Y

W

A

I

I

V

C

T

G

G

F

F

I

I

A

T

F

V

M

V

I

G

M

A

R

L

Q

C

L

Y

G

A

E

E

M

L

I

G

V

V

E

T

E

I

P

P

V

K

A

S

G

G

S

G

F

D

S

Y

H

S

F

Y

A

V

H

K

K

D

Y

I

W

F

G

G

F

L

A

A

G

G

F

F

L

L

S

R

G

L

W

W

N

I

C

A

W

-

I

V

L

L

Y

V

I

I

L

Y

V

P

G

T

M
x
N

S

Q

E

A

L

V

T

I

A

A

V

L

G

T

K

F

Y

S

I

N

H

Y

Y

V

W

L

A

Q

P

P

D

L

I

F

P

P

T

T

W

C

V

F

S

P

A

P

A

E

A

S

-

G

-

L

-

R

-

L

-

A

-

I

F

C

F

L

I

L

L

L

I

L

N

T

A

W

I

V

N

N

L

C

A

S

N

S

V

V

K

R

V

W

F

A

G

T

E

R

A

V

E

Q

F

D

W

I

F

F

A

T

I

A

I

G

K

K

V

L

A

A

I

L

V

A

G

L

M

I

I

I

A

I

L

M

G

G

S

I

Y

V

L

Q

L

I

V

C

S

K

G

G

H

E

G

Y

-

F

-

W

-

L

G

E

P

P

Q

K

A

N

S

A

V

F

T

E

N

N

L

-

W

-

S

-

H

-

G

-

F

-

F

F

P

Q

N

E

G

P

V

D

S

I

G

G

L

L

V

V

M

-

A

A

M

L

A

A

I

F

I

L

M

Q

-

G

-

S

F
|
F

S

A

F

Y

G
|
G

G

G

L

W

E

N

M

F

L

L

G

N

F

Y

T

V

A

T

A

E

E

E

A

L

D

V

K

D

P

P

K

Y

T

K

V

N

I

L

P

P

K

R

A

A

I

I

N

F

Q

I

V

S

I

I

Y

P

R

L

I

V

L

T

I

F

F

V

Y

Y

I

V

G

F

A

A

L

N

V

V

V

A

L

Y

L

V

S

T

L

A

T

M

P

S

W

P

D

Q

S

E

L

L

A

A

T

S

L

-

N

N

A

A

S

V

G

A

D

V

A

T

Y

F

S

G

G

E

S

-

P

-

F

-

V

-

Q

-

V

-

F

-

S

K

M

L

L

L

G

G

S

-

N

-

T

V

A

M

A

A

H

W

I

I

L

M

N

P

F

I

V

S

V

V

L

A

T

L

A

S

A

T

L

F

S

G

V

G

Y

V

N

N

S

G

G

S

T

L

Y

F

C

T

N

S

S

S

R

R

M

L

L

F

G

A

M

G

A

A

E

R

Q

E

G

G

D

H

A

L

P

P

K

S

A

V

L

L

S

A

R

M

I

I

D

H

-

V

K

K

R

R

G

C

V

T

P

P

V

I

R

P

S

A

I

L

L

L

A

F

S

T

A

C

A

I

V

S

T

T

L

L

V

L

binding leucine: I13 (≠ A28), G15 (= G30), G17 (= G32), N94 (≠ M108), F203 (= F212), G206 (= G215)

7cmhB The lat2-4f2hc complex in complex with tryptophan (see paper)
24% identity, 71% coverage: 23:355/471 of query aligns to 8:337/458 of 7cmhB

query
sites
7cmhB

I

I

A

I

L

V

G

G

G

N

A

I

I

I

G
|
G

T

S

G
|
G

L

I

F

F

L

V

G

S

S

P

A

K

G

G

V

V

L

L

K

E

S

N

A

A

G

G

P

S

S

V

-

G

-

L

M

A

I

L

L

I

G

V

Y

W

A

I

I

V

C

T

G

G

F

F

I

I

A

T

F

V

M

V

I

G

M

A

R

L

Q

C

L

Y

G

A

E

E

M

L

I

G

V

V

E

T

E

I

P

P

V

K

A

S

G

G

S

G

F

D

S

Y

H

S

F

Y

A

V

H

K

K

D

Y

I

W

F

G

G

F

L

A

A

G

G

F

F

L

L

S

R

G

L

W

W

N

I

C

A

W

-

I

V

L

L

Y

V

I

I

L

Y

V

P

G

T

M
x
N

S

Q

E

A

L

V

T

I

A

A

V

L

G

T

K

F

Y

S

I

N

H

Y

Y

V

W

L

A

Q

P

P

D

L

I

F

P

P

T

T

W

C

V

F

S

P

A

P

A

E

A

S

-

G

-

L

-

R

-

L

-

A

-

I

F

C

F

L

I

L

L

L

I

L

N

T

A

W

I

V

N

N

L

C

A

S

N

S

V

V

K

R

V

W

F

A

G

T

E

R

A

V

E

Q

F

D

W

I

F

F

A

T

I

A

I

G

K

K

V

L

A

A

I

L

V

A

G

L

M

I

I

I

A

I

L

M

G

G

S

I

Y

V

L

Q

L

I

V

C

S

K

G

G

H

E

G

Y

-

F

-

W

-

L

G

E

P

P

Q

K

A

N

S

A

V

F

T

E

N

N

L

-

W

-

S

-

H

-

G

-

F

-

F

F

P

Q

N

E

G

P

V

D

S

I

G

G

L

L

V

V

M

-

A

A

M

L

A

A

I

F

I

L

M

Q

-

G

-

S

F
|
F

S
x
A

F

Y

G
|
G

G

G

L

W

E

N

M

F

L

L

G

N

F

Y

T

V

A

T

A

E

E

E

A

L

D

V

K

D

P

P

K

Y

T

K

V

N

I

L

P

P

K

R

A

A

I

I

N

F

Q

I

V

S

I

I

Y

P

R

L

I

V

L

T

I

F

F

V

Y

Y

I

V

G

F

A

A

L

N

V

V

V

A

L

Y

L

V

S

T

L

A

T

M

P

S

W

P

D

Q

S

E

L

L

A

A

T

S

L

-

N

N

A

A

S

V

G

A

D

V

A

T

Y

F

S

G

G

E

S

-

P

-

F

-

V

-

Q

-

V

-

F

-

S

K

M

L

L

L

G

G

S

-

N

-

T

V

A

M

A

A

H

W

I

I

L

M

N

P

F

I

V

S

V

V

L

A

T

L

A

S

A

T

L

F

S

G

V

G

Y

V

N

N

S

G

G

S

T

L

Y

F

C

T

N

S

S

S

R

R

M

L

L

F

G

A

M

G

A

A

E

R

Q

E

G

G

D

H

A

L

P

P

K

S

A

V

L

L

S

A

R

M

I

I

D

H

-

V

K

K

R

R

G

C

V

T

P

P

V

I

R

P

S

A

I

L

L

L

A

F

S

T

A

C

A

I

V

S

T

T

L

L

V

L

binding tryptophan: G15 (= G30), G17 (= G32), N94 (≠ M108), F203 (= F212), A204 (≠ S213), G206 (= G215)

Sites not aligning to the query:

binding tryptophan: 355

7b00A Human lat2-4f2hc complex in the apo-state (see paper)
24% identity, 71% coverage: 23:355/471 of query aligns to 8:337/457 of 7b00A

query
sites
7b00A

I

I

A

I

L

V

G

G

G

N

A

I

I

I

G

G

T

S

G

G

L

I

F

F

L

V

G

S

S

P

A

K

G

G

V

V

L

L

K

E

S

N

A

A

G

G

P

S

S

V

-

G

-

L

M

A

I

L

L

I

G

V

Y

W

A

I

I

V

C

T

G

G

F

F

I

I

A

T

F

V

M

V

I

G

M

A

R

L

Q

C

L

Y

G

A

E

E

M

L

I

G

V

V

E

T

E

I

P

P

V

K

A

S

G

G

S

G

F

D

S

Y

H

S

F

Y

A

V

H

K

K

D

Y

I

W

F

G

G

F

L

A

A

G

G

F

F

L

L

S

R

G

L

W

W

N

I

C

A

W

-

I

V

L

L

Y

V

I

I

L

Y

V

P

G

T

M

N

S

Q

E

A

L

V

T

I

A

A

V

L

G

T

K

F

Y

S

I

N

H

Y

Y

V

W

L

A

Q

P

P

D

L

I

F

P

P

T

T

W

C

V

F

S

P

A

P

A

E

A

S

-

G

-

L

-

R

-

L

-

A

-

I

F

C

F

L

I

L

L

L

I

L

N

T

A

W

I

V

N

N

L

C

A

S

N

S

V

V

K

R

V

W

F

A

G

T

E

R

A

V

E

Q

F

D

W

I

F

F

A

T

I

A

I

G

K

K

V

L

A

A

I

L

V

A

G

L

M

I

I

I

A

I

L

M

G

G

S

I

Y

V

L

Q

L

I

V

C

S

K

G

G

H

E

G

Y

-

F

-

W

-

L

G

E

P

P

Q

K

A

N

S

A

V

F

T

E

N

N

L

-

W

-

S

-

H

-

G

-

F

-

F

F

P

Q

N

E

G

P

V

D

S

I

G

G

L

L

V

V

M

-

A

A

M

L

A

A

I

F

I

L

M

Q

-

G

-

S

F

F

S

A

F

Y

G

G

L

W

E

N

M

F

L

L

G

N

F

Y

T

V

A

T

A

E

E

E

A

L

D

V

K

D

P

P

K

Y

T

K

V

N

I

L

P

P

K

R

A

A

I

I

N

F

Q

I

V

S

I

I

Y

P

R

L

I

V

L

T

I

F

F

V

Y

Y

I

V

G

F

A

A

L

N

V

V

V

A

L

Y

L

V

S

T

L

A

T

M

P

S

W

P

D

Q

S

E

L

L

A

A

T

S

L

-

N

N

A

A

S

V

G

A

D

V

A

T

Y

F

S

G

G

E

S

-

P

-

F

-

V

-

Q

-

V

-

F

-

S

K

M

L

L

L

G

G

S

-

N

-

T

V

A

M

A

A

H

W

I

I

L

M

N

P

F

I

V

S

V

V

L

A

T

L

A

S

A

T

L

F

S

G

V

G

Y

V

N

N

S

G

G

S

T

L

Y

F

C

T

N

S

S

S

R

R

M

L

L

F

G

A

M

G

A

A

E

R

Q

E

G

G

D

H

A

L

P

P

K

S

A

V

L

L

S

A

R

M

I

I

D

H

-

V

K

K

R

R

G

C

V

T

P

P

V

I

R

P

S

A

I

L

L

L

A

F

S

T

A

C

A

I

V

S

T

T

L

L

V

L

Sites not aligning to the query:

binding Digitonin: 338, 350, 408, 411

Q9UHI5 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; hLAT2; Solute carrier family 7 member 8 from Homo sapiens (Human) (see 3 papers)
24% identity, 71% coverage: 23:355/471 of query aligns to 48:377/535 of Q9UHI5

query
sites
Q9UHI5

I

I

A

I

L

V

G

G

G

N

A
x
I

I

I

G

G

T

S

G

G

L

I

F

F

L

V

G

S

S

P

A

K

G

G

V

V

L

L

K

E

S

N

A

A

G

G

P

S

S

V

-

G

-

L

M

A

I

L

L

I

G

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Y

W

A

I

I

V

C

T

G

G

F

F

I

I

A

T

F

V

M

V

I

G

M

A

R

L

Q

C

L
x
Y

G

A

E

E

M

L

I

G

V

V

E

T

E

I

P

P

V

K

A

S

G

G

S

G

F

D

S

Y

H

S

F

Y

A

V

H

K

K

D

Y

I

W

F

G

G

F

L

A

A

G

G

F

F

L

L

S

R

G

L

W

W

N

I

C

A

W

-

I

V

L

L

Y

V

I

I

L

Y

V

P

G

T

M
x
N

S

Q

E

A

L

V

T

I

A

A

V

L

G

T

K

F

Y

S

I

N

H

Y

Y

V

W

L

A

Q

P

P

D

L

I

F

P

P

T

T

W
x
C

V

F

S

P

A

P

A

E

A

S

-

G

-

L

-

R

-

L

-

A

-

I

F

C

F

L

I

L

L

L

I

L

N

T

A
x
W

I

V

N

N

L

C

A

S

N

S

V

V

K

R

V

W

F

A

G

T

E

R

A

V

E

Q

F

D

W

I

F

F

A

T

I

A

I

G

K

K

V

L

A

A

I

L

V

A

G

L

M

I

I

I

A

I

L

M

G

G

S

I

Y

V

L

Q

L

I

V

C

S

K

G

G

H

E

G

Y

-

F

-

W

-

L

G

E

P

P

Q

K

A

N

S

A

V

F

T

E

N

N

L

-

W

-

S

-

H

-

G

-

F

-

F

F

P

Q

N

E

G

P

V

D

S

I

G

G

L

L

V

V

M

-

A

A

M

L

A

A

I

F

I

L

M

Q

-

G

-

S

F
|
F

S

A

F

Y

G
|
G

G

G

L

W

E

N

M

F

L

L

G

N

F

Y

T

V

A

T

A

E

E

E

A

L

D

V

K

D

P

P

K

Y

T

K

V

N

I

L

P

P

K

R

A

A

I

I

N

F

Q

I

V

S

I

I

Y

P

R

L

I

V

L

T

I

F

F

V

Y

Y

I

V

G

F

A

A

L

N

V

V

V

A

L

Y

L

V

S

T

L

A

T

M

P

S

W

P

D

Q

S

E

L

L

A

A

T

S

L

-

N

N

A

A

S
x
V

G

A

D

V

A

T

Y

F

S

G

G

E

S

-

P

-

F

-

V

-

Q

-

V

-

F

-

S

K

M

L

L

L

G

G

S

-

N

-

T

V

A

M

A

A

H

W

I

I

L

M

N

P

F

I

V

S

V

V

L

A

T

L

A

S

A

T

L

F

S

G

V

G

Y

V

N

N

S

G

G

S

T

L

Y

F

C

T

N

S

S

S

R

R

M

L

L

F

G

A

M

G

A

A

E

R

Q

E

G

G

D

H

A

L

P

P

K

S

A

V

L

L

S

A

R

M

I

I

D

H

-

V

K

K

R

R

G

C

V

T

P

P

V

I

R

P

S

A

I

L

L

L

A

F

S

T

A

C

A

I

V

S

T

T

L

L

V

L

I53 (≠ A28) binding L-leucine
Y93 (≠ L66) mutation to A: Nearly complete reduction of glycine, L-alanine, and L-glutamine uptake. Minimal effect on the transport of L-isoleucine, L-histidine and L-tryptophan.
N134 (≠ M108) Important for substrate specificity; binding L-tryptophan; mutation to Q: Reduces L-leucine uptake activity. Abolishes L-tryptophan uptake.; mutation to S: The substrate specificity changed dramatically reducing L-glutamine, glycine and L-alanine uptake activity thus mimicking the selectivity of SLC7A5.
C154 (≠ W128) modified: Interchain (with C-210 in SLC3A2)
W174 (≠ A140) mutation to A: Does not affect protein expression, plasma membrane localization, or L-alanine uptake.
F243 (= F212) mutation to A: Abolishes leucine and tryptophan transport activities.
G246 (= G215) Important for substrate specificity; binding L-leucine; mutation to S: Strong decrease in the uptake of large substrates L-tryptophan, L-glutamine, and L-histidine but increases the uptake of small neutral amino acids glycine and L-alanine.
V302 (≠ S272) to I: found in a patient with age-related hearing loss; does not affect L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs142951280

Sites not aligning to the query:

395 binding L-tryptophan; N→Q: Strongly reduces L-leucine uptake activity. Strongly reduces L-tryptophan uptake activity.
396 Y→A: Strongly reduces L-leucine uptake activity.
402 T → M: found in a patient with age-related hearing loss; strongly decreased L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs758342760
418 R → C: found in a patient with age-related hearing loss; decreases L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs146946494
460 V → E: found in a patient with age-related hearing loss; strongly decreases L-alanine transport activity. Decreases L-tyrosine transport activity. Decreases cell membrane localization; dbSNP:rs2048595742

Q9QXW9 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; mLAT2; Solute carrier family 7 member 8 from Mus musculus (Mouse) (see paper)
24% identity, 71% coverage: 23:355/471 of query aligns to 47:376/531 of Q9QXW9

query
sites
Q9QXW9

I

I

A

I

L

V

G

G

G

N

A

I

I

I

G

G

T

S

G

G

L

I

F

F

L

V

G

S

S

P

A

K

G

G

V

V

L

L

K

E

S

N

A

A

G

G

P

S

S

-

M

-

I

-

L

V

G

G

Y

L

A

A

I

L

C

I

G

V

F

W

I

I

A

V

F

T

M

G

I

I

M

I

R

T

Q

A

L

V

G

G

E

A

M

L

I

C

V

Y

E

A

E

E

-

L

-

G

-

V

-

T

-

I

P

P

V

K

A

S

G

G

S

G

F

D

S

Y

H

S

F

Y

A

V

H

K

K

D

Y

I

W

F

G

G

F

L

A

A

G

G

F

F

L

L

S

R

G

L

W

W

N

I

C

A

W

-

I

V

L

L

Y

V

I

I

L
x
Y

V

P

G

T

M
x
N

S

Q

E

A

L

V

T

I

A

A

V

L

G

T

K

F

Y

S

I

N

H

Y

Y

V

W

L

A

Q

P

P

D

L

I

F

P

P

T

T

W

C

V

F

S

P

A

P

A

E

A

S

-

G

-

L

-

R

-

L

-

A

-

I

F

C

F

L

I

L

L

L

I

L

N

T

A

W

I

V

N

N

L

C

A

S

N

S

V

V

K

R

V

W

F

A

G

T

E

R

A

V

E

Q

F

D

W

I

F

F

A

T

I

A

I

G

K

K

V

L

A

A

I

L

V

A

G

L

M

I

I

I

A

I

L

M

G

G

S

I

Y

V

L

Q

L

I

V

C

S

K

G

G

H

E

G

F

-

F

-

W

-

L

G

E

P

P

Q

K

A

N

S

A

V

F

T

E

N

N

L

-

W

-

S

-

H

-

G

-

F

-

F

F

P

Q

N

E

G

P

V

D

S

I

G

G

L

L

V

V

M

-

A

A

M

L

A

A

I

F

I

L

M

Q

-

G

-

S

F
|
F

S

A

F

Y

G

G

L

W

E

N

M

F

L

L

G

N

F

Y

T

V

A

T

A

E

E

E

A

L

D

V

K

D

P

P

K

Y

T

K

V

N

I

L

P

P

K

R

A

A

I

I

N

F

Q

I

V

S

I

I

Y

P

R

L

I

V

L

T

I

F

F

V

Y

Y

I

V

G

F

A

A

L

N

V

I

V

A

L

Y

L

V

S

T

L

A

T

M

P

S

W

P

D

Q

S

E

L

L

A

A

T

S

L

-

N

N

A

A

S

V

G

A

D

V

A

T

Y

F

S

G

G

E

S

-

P

-

F

-

V

-

Q

-

V

-

F

-

S

K

M

L

L

L

G

G

S

-

N

-

T

V

A

M

A

A

H

W

I

I

L

M

N

P

F

I

V

S

V

V

L

A

T

L

A

S

A

T

L

F

S

G

V

G

Y

V

N

N

S

G

G

S

T

L

Y

F

C

T

N

S

S

S

R

R

M

L

L

F

G

A

M

G

A

A

E

R

Q

E

G

G

D

H

A

L

P

P

K

S

A

V

L

L

S

A

R

M

I

I

D

H

-

V

K

K

R

R

G

C

V

T

P

P

V

I

R

P

S

A

I

L

L

L

A

F

S

T

A

C

A

L

V

S

T

T

L

L

V

L

Y130 (≠ L105) mutation to A: Increases T2 import. Increases T3 and enables T4 import. Does not affect L-leucine and L-phenylalanine uptake.
N133 (≠ M108) mutation to S: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake. Increases the export of both L-leucine and L-phenylalanine.
F242 (= F212) mutation to W: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake.

P60061 Arginine/agmatine antiporter from Escherichia coli (strain K12) (see 3 papers)
22% identity, 70% coverage: 20:347/471 of query aligns to 15:328/445 of P60061

query
sites
P60061

I

V

Q

T

L

L

I

M

A

V

L

S

G

G

G

N

A
x
I

I

M

G

G

T
x
S

G

G

L

V

F

F

L

L

G

L

S

P

A

A

G

N

V

-

L

L

K

A

S

S

A

T

G

G

P

G

S

I

M

A

I

I

L

Y

G

G

Y

W

A

L

I

V

C

T

G

I

F

I

I

G

A

A

F

L

M

G

I

L

M

S

R

M

Q

V

L

Y

G

A

E

K

M

M

I

S

V

F

E

L

E

D

P

P

V

S

A

P

G

G

S

G

F

S

S

Y

H

A

F

Y

A

A

H

R

K

R

Y

C

W

F

G

G

G

P

F

F

A

L

G

G

F

Y

L

Q

S

T

G

N

-

V

-

L

-
x
Y

W

W

-

L

N
x
A

C
|
C

W

W

I

I

L

-

Y

-

I

-

L

-

V

-

G

G

M
x
N

S

I

E

A

L
x
M

T

V

A

V

V

I

G

G

-

V

K

G

Y

Y

I

L

H

S

Y

Y

W

F

A

F

P

P

D

I

I

L

P

K

-

D

-

P

-

L

-

V

-

L

-

T

-

I

T

T

W

C

V

V

S

V

A

V

A

L

A

W

F

I

F

F

I

V

L

L

I

L

N

N

A

-

I

-

N

-

L

I

A

V

N

G

V

P

K

K

V

M

F

I

G

T

E

R

A

V

E

Q

F

A

W

V

F

A

A

T

I

V

I

L

K

A

V

L

V

I

A

P

I

I

V

V

G

G

M

I

I

A

A

V

L

F

G

G

S

W

Y

F

L

W

L

F

V

-

S

-

G

-

H

R

G

G

G

E

P

T

Q

Y

A

M

S

A

V

A

T

W

N

N

L

V

W

S

S

G

H

L

G

G

G

T

F

F

F

-

P

-

N

-

G

-

V

-

S

G

G

A

L

I

V

Q

M

S

A

T

M

L

A

N

I

V

I

T

M

L

F
x
W

S
|
S

F

F

G
x
I

G

G

L

V

E

E

M

S

L

A

G

S

F

V

T

A

A

A

A

G

E

V

A

V

D

K

K

N

P

P

K

K

T

R

V

N

I

V

P

P

K

I

A

A

I

T

N

I

Q

G

V

G

I

V

Y

L

R

I

I

A

L

A

I

V

F

C

Y

Y

I

V

G

L

A

S

L

T

V

T

V

A

L

I

L

M

S

G

L

M

T

I

P

P

W

N

D

A

S

A

L

L

L

R

A

V

T

-

L

-

N

-

A

-

S

-

G

-

D

-

A

-

Y

-

S

S

G

A

S

S

P

P

F

F

V

G

Q

D

V

A

F

A

S

R

M

M

L

A

G

L

S

G

N

D

T

T

A

A

A

G

H

A

I

I

L

V

N

S

F

F

V

C

V

A

L

A

T

A

A

G

A

C

L

L

S

G

V

S

Y

L

N

G

S

G

G
x
W

T

T

Y

L

C

L

N

A

S

G

R

Q

M

T

L

A

L

K

G

A

M

A

A

A

E

D

Q

D

G

G

D

L

A

F

P

P

K

P

A

I

L

F

S

A

R

R

I

V

D

N

K

K

R

A

G

G

V

T

P

P

V

V

R

A

S

G

I

L

L

I

I23 (≠ A28) binding agmatine; binding L-arginine
S26 (≠ T31) binding L-arginine
Y93 (vs. gap) mutation to L: Greatly decreased Arg uptake into liposomes.
A96 (≠ N98) binding agmatine; binding L-arginine
C97 (= C99) binding agmatine
N101 (≠ M108) binding agmatine; mutation to A: Vmax for Arg-Agm exchange 1% of wild-type, KM increases 3-fold.; mutation to D: Nearly wild-type Arg-Agm exchange.
M104 (≠ L111) binding agmatine; mutation to A: 30% decreased affinity for Arg, 50% decreased affinity for Agm.
W202 (≠ F212) binding L-arginine; mutation to L: Halves Arg uptake into liposomes.
S203 (= S213) binding agmatine
I205 (≠ G215) binding agmatine; binding L-arginine; mutation to A: About wild-type affinity for Arg and Agm.
W293 (≠ G312) binding agmatine; mutation W->C,H,L: Loss of Arg-Agm exchange.; mutation W->F,Y: Less than 20% Arg-Agm exchange activity. Vmax 15% of wild-type rate.

Sites not aligning to the query:

357 binding L-arginine; S→A: 20% decreased affinity for Arg, 40% decrease affinity for Agm.

Query Sequence

>AO356_18530 FitnessBrowser__pseudo5_N2C3_1:AO356_18530
MSGQNSHSGELKRGLKNRHIQLIALGGAIGTGLFLGSAGVLKSAGPSMILGYAICGFIAF
MIMRQLGEMIVEEPVAGSFSHFAHKYWGGFAGFLSGWNCWILYILVGMSELTAVGKYIHY
WAPDIPTWVSAAAFFILINAINLANVKVFGEAEFWFAIIKVVAIVGMIALGSYLLVSGHG
GPQASVTNLWSHGGFFPNGVSGLVMAMAIIMFSFGGLEMLGFTAAEADKPKTVIPKAINQ
VIYRILIFYIGALVVLLSLTPWDSLLATLNASGDAYSGSPFVQVFSMLGSNTAAHILNFV
VLTAALSVYNSGTYCNSRMLLGMAEQGDAPKALSRIDKRGVPVRSILASAAVTLVAVLLN
YLVPQHALELLMSLVVATLVINWAMISYSHFKFRQHMNQTQQTPLFKALWYPYGNYICLA
FVVFILGVMLLIPGIQISVYAIPVWVVFMWVCYVIKNKRSARQELAVAAAK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory