SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO356_19355 AO356_19355 HAD family hydrolase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

O53289 Phosphoserine phosphatase SerB2; PSP; PSPase; O-phosphoserine phosphohydrolase; Protein-serine/threonine phosphatase; EC 3.1.3.3; EC 3.1.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
25% identity, 95% coverage: 3:209/217 of query aligns to 181:371/409 of O53289

query
sites
O53289

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D185 (= D7) mutation to G: Completely abolishes enzymatic activity.; mutation to N: Completely abolishes enzymatic activity.
V186 (≠ L8) mutation to Q: Decreases enzymatic activity by 50%.
D187 (= D9) mutation to N: Decreases enzymatic activity by 15%.
S188 (≠ E10) mutation to A: No effect on enzymatic activity.
S273 (= S111) mutation to A: Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).
K318 (= K156) mutation to A: Decreases enzymatic activity by 50%.; mutation to E: Completely abolishes enzymatic activity.
D341 (= D179) mutation to G: Decreases enzymatic activity by 80%.; mutation to N: Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.
D345 (= D183) mutation to N: Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.

Sites not aligning to the query:

18 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
108 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.

A0QJI1 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Mycobacterium avium (strain 104) (see paper)
24% identity, 95% coverage: 3:209/217 of query aligns to 183:373/411 of A0QJI1

query
sites
A0QJI1

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D187 (= D7) binding
D189 (= D9) binding
D343 (= D179) binding

8a21A Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with phenylimidazole (see paper)
24% identity, 95% coverage: 3:209/217 of query aligns to 179:369/396 of 8a21A

query
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8a21A

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binding magnesium ion: D183 (= D7), D185 (= D9), D339 (= D179)
binding 4-phenyl-1h-imidazole: D185 (= D9), E192 (≠ D16), V193 (≠ C17), I194 (≠ A18), T211 (≠ M44), M215 (≠ S48), F221 (≠ M54), R228 (≠ S61), G273 (≠ S113)

Sites not aligning to the query:

binding 4-phenyl-1h-imidazole: 13, 18, 37

8a1zA Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with 1-(2,4-dichlorophenyl)-3-hydroxyurea (see paper)
24% identity, 95% coverage: 3:209/217 of query aligns to 179:369/396 of 8a1zA

query
sites
8a1zA

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binding 1-(2,4-dichlorophenyl)-3-oxidanyl-urea: D185 (= D9), E192 (≠ D16), M215 (≠ S48), F221 (≠ M54), L225 (≠ M58), R228 (≠ S61), G272 (≠ A112), F274 (≠ G114), D339 (= D179)
binding magnesium ion: D183 (= D7), D185 (= D9), D339 (= D179)

5jlpA Crystal structure of mycobacterium avium serb2 in complex with serine at act domain
24% identity, 95% coverage: 3:209/217 of query aligns to 179:369/396 of 5jlpA

query
sites
5jlpA

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active site: D183 (= D7), V184 (≠ L8), D185 (= D9), G272 (≠ A112), K316 (= K156), D343 (= D183)
binding magnesium ion: D183 (= D7), D339 (= D179)

Sites not aligning to the query:

binding serine: 13, 14

1l7nA Transition state analogue of phosphoserine phosphatase (aluminum fluoride complex) (see paper)
25% identity, 94% coverage: 3:207/217 of query aligns to 5:193/209 of 1l7nA

query
sites
1l7nA

L

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K

E

T

T

I

I

A

K

E

E

H

L

R

K

K

N

A

R

G

G

D

Y

R

V

I

V

L

A

V

V

I

V

S
|
S

A
x
G

S

G

G

F

T

D

H

I

L

A

V

V

K

N

P

K

I

I

A

K

E

E

R

K

L

L

G

G

I

L

D

D

E

Y

I

A

L

F

A

A

I

N

E

R

L

L

E

I

V

V

A

K

H

D

G

G

V

K

Y

L

S

T

G

G

K

D

T

V

V

E

G

G

T

E

L

V

T

L

Y

K

R

E

E

N

G

A

K
|
K

I

G

A

E

R

I

L

L

L

E

D

K

W

I

L

A

D

K

A

I

E

E

E

G

E

I

N

N

L

L

E

E

G

D

A

T

S

V

F

A

Y

V

S

G

D
|
D

S

G

R

A

N
|
N

D
|
D

L

I

P

S

L

M

L

F

L

K

K

K

V

A

D

G

H

L

P

K

H

I

V

A

V

F

N

C

P

A

D

K

P

P

V

I

L

L

K

K

A

E

H

K

A

A

E

D

active site: D9 (= D7), F10 (≠ L8), D11 (= D9), G98 (≠ A112), K142 (= K156), D169 (= D183)
binding aluminum fluoride: D9 (= D7), F10 (≠ L8), D11 (= D9), S97 (= S111), K142 (= K156)
binding tetrafluoroaluminate ion: D9 (= D7), F10 (≠ L8), D11 (= D9), S97 (= S111), G98 (≠ A112), K142 (= K156), N168 (= N182)
binding magnesium ion: D9 (= D7), D11 (= D9), D165 (= D179)

1f5sA Crystal structure of phosphoserine phosphatase from methanococcus jannaschii (see paper)
25% identity, 94% coverage: 3:207/217 of query aligns to 6:194/210 of 1f5sA

query
sites
1f5sA

L

L

A

I

I

L

F

F

D
|
D

L
x
F

D
|
D

E

S

T

T

L

L

I

V

H

N

G

N

D

E

C

T

A

I

T

-

L

-

W

-

S

-

E

D

Q

E

M

I

G

A

R

R

L

E

G

A

W

G

V

V

D

E

P

-

E

E

S

E

F

V

M

K

R

K

R

I

N

T

N

K

E

E

L

A

M

M

D

E

A

-

Y

-

S

-

H

-

G

G

K

K

L

L

S

N

M

F

E

E

E

Q

Y

S

M

L

D

R

F

K

S

R

L

V

E

S

P

L

M

L

I

K

G

D

R

L

T

P

P

I

E

E

E

K

I

V

E

E

H

K

L

A

V

I

A

K

P

R

W

-

V

-

E

-

D

-

F

-

I

I

E

T

P

P

I

T

I

-

F

-

S

E

D

G

A

A

T

E

K

E

T

T

I

I

A

K

E

E

H

L

R

K

K

N

A

R

G

G

D

Y

R

V

I

V

L

A

V

V

I

V

S
|
S

A
x
G

S

G

G

F

T

D

H

I

L

A

V

V

K

N

P

K

I

I

A

K

E

E

R

K

L

L

G

G

I

L

D

D

E

Y

I

A

L

F

A

A

I

N

E

R

L

L

E

I

V

V

A

K

H

D

G

G

V

K

Y

L

S

T

G

G

K

D

T

V

V

E

G

G

T

E

L

V

T

L

Y

K

R

E

E

N

G

A

K
|
K

I

G

A

E

R

I

L

L

L

E

D

K

W

I

L

A

D

K

A

I

E

E

E

G

E

I

N

N

L

L

E

E

G

D

A

T

S

V

F

A

Y

V

S

G

D
|
D

S

G

R

A

N

N

D
|
D

L

I

P

S

L

M

L

F

L

K

K

K

V

A

D

G

H

L

P

K

H

I

V

A

V

F

N

C

P

A

D

K

P

P

V

I

L

L

K

K

A

E

H

K

A

A

E

D

active site: D10 (= D7), F11 (≠ L8), D12 (= D9), G99 (≠ A112), K143 (= K156), D170 (= D183)
binding magnesium ion: D10 (= D7), D12 (= D9), D166 (= D179)
binding phosphate ion: D10 (= D7), F11 (≠ L8), D12 (= D9), S98 (= S111), G99 (≠ A112), K143 (= K156)

Q58989 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see 3 papers)
25% identity, 94% coverage: 3:207/217 of query aligns to 7:195/211 of Q58989

query
sites
Q58989

L

L

A

I

I

L

F

F

D
|
D

L

F

D
|
D

E

S

T

T

L

L

I

V

H

N

G

N

D
x
E

C

T

A

I

T

-

L

-

W

-

S

-

E

D

Q

E

M

I

G

A

R

R

L

E

G

A

W

G

V

V

D

E

P

-

E

E

S

E

F

V

M

K

R

K

R

I

N

T

N

K

E

E

L

A

M

M

D

E

A

-

Y

-

S

-

H

-

G

G

K

K

L

L

S

N

M

F

E

E

E

Q

Y

S

M

L

D

R

F

K

S
x
R

L

V

E

S

P

L

M

L

I

K

G

D

R

L

T

P

P

I

E

E

E

K

I

V

E

E

H

K

L

A

V

I

A

K

P

R

W

-

V

-

E

-

D

-

F

-

I

I

E

T

P

P

I

T

I

-

F

-

S

E

D

G

A

A

T

E

K

E

T

T

I

I

A

K

E

E

H

L

R

K

K

N

A

R

G

G

D

Y

R

V

I

V

L

A

V

V

I

V

S
|
S

A
x
G

S

G

G

F

T

D

H

I

L

A

V

V

K

N

P

K

I

I

A

K

E

E

R

K

L

L

G

G

I

L

D

D

E

Y

I

A

L

F

A

A

I

N

E

R

L

L

E

I

V

V

A

K

H

D

G

G

V

K

Y

L

S

T

G

G

K

D

T

V

V

E

G

G

T

E

L

V

T

L

Y

K

R

E

E

N

G

A

K
|
K

I

G

A

E

R

I

L

L

L

E

D

K

W

I

L

A

D

K

A

I

E

E

E

G

E

I

N

N

L

L

E

E

G

D

A

T

S

V

F

A

Y

V

S

G

D
|
D

S

G

R

A

N
|
N

D

D

L

I

P

S

L

M

L

F

L

K

K

K

V

A

D

G

H

L

P

K

H

I

V

A

V

F

N

C

P

A

D

K

P

P

V

I

L

L

K

K

A

E

H

K

A

A

E

D

D11 (= D7) active site, Nucleophile; binding ; mutation to N: Loss of activity.
D13 (= D9) active site, Proton donor; binding
E20 (≠ D16) binding
R56 (≠ S61) binding
SG 99:100 (≠ SA 111:112) binding
K144 (= K156) binding
D167 (= D179) binding
N170 (= N182) binding

Query Sequence

>AO356_19355 AO356_19355 HAD family hydrolase
MALAIFDLDETLIHGDCATLWSEQMGRLGWVDPESFMRRNNELMDAYSHGKLSMEEYMDF
SLEPMIGRTPEEIEHLVAPWVEDFIEPIIFSDATKTIAEHRKAGDRILVISASGTHLVKP
IAERLGIDEILAIELEVAHGVYSGKTVGTLTYREGKIARLLDWLDAEEENLEGASFYSDS
RNDLPLLLKVDHPHVVNPDPVLKAHAESAGWPIHSWK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory