SitesBLAST
Comparing AO356_20290 AO356_20290 galactonate dehydratase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q6BF17 D-galactonate dehydratase; GalD; EC 4.2.1.6 from Escherichia coli (strain K12)
62% identity, 100% coverage: 1:382/382 of query aligns to 1:382/382 of Q6BF17
- H185 (= H185) mutation H->N,Q: Loss of activity.
- H285 (= H285) mutation to N: Loss of activity.
- E310 (= E310) mutation to Q: Loss of activity.
3rraB Crystal structure of enolase prk14017 (target efi-500653) from ralstonia pickettii 12j with magnesium bound
63% identity, 100% coverage: 1:382/382 of query aligns to 2:375/379 of 3rraB
- active site: I35 (≠ V34), R38 (= R37), Y118 (= Y117), K145 (= K144), N147 (= N146), E151 (= E150), D184 (= D183), H186 (= H185), E210 (= E209), G235 (= G234), E236 (= E235), R237 (= R236), Q257 (= Q256), D259 (= D258), H286 (= H285), P288 (= P287), E311 (= E310)
- binding magnesium ion: D184 (= D183), E210 (= E209), E236 (= E235)
4e4fB Crystal structure of enolase pc1_0802 (target efi-502240) from pectobacterium carotovorum subsp. Carotovorum pc1
34% identity, 100% coverage: 1:382/382 of query aligns to 2:381/381 of 4e4fB
- active site: L37 (≠ V34), R40 (= R37), R148 (≠ K144), Q150 (≠ N146), D189 (= D183), H191 (= H185), E215 (= E209), G240 (= G234), E241 (= E235), V242 (≠ R236), R262 (≠ Q256), T264 (≠ D258), H291 (= H285), P293 (= P287), E318 (= E310), W381 (= W382)
- binding magnesium ion: D189 (= D183), E215 (= E209), E241 (= E235)
3v3wA Crystal structure of an enolase from the soil bacterium cellvibrio japonicus (target efi-502161) with bound mg and glycerol
32% identity, 100% coverage: 1:382/382 of query aligns to 1:397/397 of 3v3wA
- active site: L36 (≠ V34), R39 (= R37), H122 (≠ W119), K144 (≠ T141), R147 (≠ K144), Q149 (≠ N146), Y159 (≠ F153), E179 (vs. gap), D205 (= D183), H207 (= H185), E231 (= E209), G256 (= G234), E257 (= E235), V258 (≠ R236), R278 (≠ Q256), T280 (≠ D258), F306 (≠ L284), H307 (= H285), G308 (vs. gap), A309 (vs. gap), E334 (= E310), W397 (= W382)
- binding magnesium ion: D205 (= D183), E231 (= E209), E257 (= E235)
B3PDB1 D-galactonate dehydratase family member RspA; D-mannonate dehydratase; Starvation sensing protein RspA homolog; EC 4.2.1.-; EC 4.2.1.8 from Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) (see paper)
32% identity, 100% coverage: 1:382/382 of query aligns to 1:402/402 of B3PDB1
- D210 (= D183) binding
- E236 (= E209) binding
- E262 (= E235) binding
2o56A Crystal structure of a member of the enolase superfamily from salmonella typhimurium
34% identity, 95% coverage: 1:363/382 of query aligns to 2:389/392 of 2o56A
- active site: K165 (= K144), D167 (≠ N146), M171 (vs. gap), L186 (vs. gap), E214 (≠ D183), H216 (= H185), E240 (= E209), G265 (= G234), E266 (= E235), Q287 (= Q256), D289 (= D258), H316 (= H285), E341 (= E310), A346 (≠ I315)
- binding magnesium ion: E214 (≠ D183), E240 (= E209), E266 (= E235)
3rgtA Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with d-arabinohydroxamate
33% identity, 100% coverage: 1:382/382 of query aligns to 1:383/383 of 3rgtA
- active site: H122 (≠ W119), R147 (≠ K144), Q149 (≠ N146), D191 (= D183), H193 (= H185), E217 (= E209), G242 (= G234), E243 (= E235), R264 (≠ Q256), P266 (≠ D258), H293 (= H285), G294 (≠ C286), E320 (= E310), W383 (= W382)
- binding cobalt (ii) ion: D191 (= D183), E217 (= E209), E243 (= E235)
- binding (2S,3R,4R)-2,3,4,5-tetrahydroxy-N-oxo-pentanamide: N37 (≠ E35), D191 (= D183), H193 (= H185), E243 (= E235), H293 (= H285), P295 (= P287), D297 (vs. gap), E320 (= E310), W383 (= W382)
3thuA Crystal structure of an enolase from sphingomonas sp. Ska58 (efi target efi-501683) with bound mg
31% identity, 100% coverage: 2:382/382 of query aligns to 5:405/405 of 3thuA
- active site: L39 (≠ V34), E43 (≠ A38), W79 (≠ Y73), G124 (≠ S118), R150 (≠ K144), Q152 (≠ N146), Y162 (vs. gap), D213 (= D183), H215 (= H185), E239 (= E209), G264 (= G234), E265 (= E235), R286 (≠ Q256), T288 (≠ D258), H315 (= H285), E342 (= E310), W405 (= W382)
- binding magnesium ion: D213 (= D183), E239 (= E209), E265 (= E235)
Q1NAJ2 D-mannonate dehydratase; ManD; EC 4.2.1.8 from Sphingomonas sp. (strain SKA58) (see paper)
31% identity, 100% coverage: 2:382/382 of query aligns to 3:403/403 of Q1NAJ2
- D211 (= D183) binding
- E237 (= E209) binding
- E263 (= E235) binding
4gmeC Crystal structure of mannonate dehydratase (target efi-502209) from caulobacter crescentus cb15 complexed with magnesium and d-mannonate
31% identity, 100% coverage: 1:382/382 of query aligns to 2:403/403 of 4gmeC
- active site: L37 (≠ V34), R40 (= R37), D211 (= D183), H213 (= H185), E237 (= E209), G262 (= G234), E263 (= E235), I264 (≠ R236), T286 (≠ D258), H313 (= H285), A315 (vs. gap), E340 (= E310)
- binding d-mannonic acid: N38 (≠ E35), Y160 (vs. gap), D211 (= D183), H213 (= H185), E237 (= E209), E263 (= E235), H313 (= H285), D317 (≠ P287), E340 (= E310), L390 (≠ N369), W403 (= W382)
- binding magnesium ion: D211 (= D183), E237 (= E209), E263 (= E235)
4gmeA Crystal structure of mannonate dehydratase (target efi-502209) from caulobacter crescentus cb15 complexed with magnesium and d-mannonate
31% identity, 100% coverage: 1:382/382 of query aligns to 2:403/403 of 4gmeA
- active site: L37 (≠ V34), R40 (= R37), T119 (≠ R115), R148 (≠ K144), Q150 (≠ N146), Y160 (vs. gap), D211 (= D183), H213 (= H185), E237 (= E209), G262 (= G234), E263 (= E235), I264 (≠ R236), R284 (≠ Q256), T286 (≠ D258), H313 (= H285), A315 (vs. gap), E340 (= E310), W403 (= W382)
- binding carbonate ion: R148 (≠ K144), Y160 (vs. gap), D211 (= D183), E263 (= E235), E340 (= E310)
- binding magnesium ion: D211 (= D183), E237 (= E209), E263 (= E235)
Q9AAR4 D-mannonate dehydratase CC0532; ManD; EC 4.2.1.8 from Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus) (see paper)
31% identity, 100% coverage: 1:382/382 of query aligns to 2:403/403 of Q9AAR4
- D211 (= D183) binding
- E237 (= E209) binding
- E263 (= E235) binding
Q8FHC7 D-galactonate dehydratase family member RspA; D-mannonate dehydratase; Starvation sensing protein RspA homolog; EC 4.2.1.-; EC 4.2.1.8 from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) (see paper)
32% identity, 100% coverage: 1:382/382 of query aligns to 12:415/415 of Q8FHC7
- D223 (= D183) binding
- E249 (= E209) binding
- D250 (≠ E210) binding
- E275 (= E235) binding
4il2B Crystal structure of d-mannonate dehydratase (rspa) from e. Coli cft073 (efi target efi-501585)
32% identity, 100% coverage: 1:382/382 of query aligns to 1:404/404 of 4il2B
- active site: L36 (≠ V34), G38 (= G36), W76 (≠ Y73), R147 (≠ K144), Q149 (vs. gap), Y159 (vs. gap), D212 (= D183), H214 (= H185), E238 (= E209), G263 (= G234), E264 (= E235), R285 (≠ Q256), T287 (≠ D258), H314 (= H285), E341 (= E310), W404 (= W382)
- binding magnesium ion: D212 (= D183), E238 (= E209), D239 (≠ E210), E264 (= E235)
3p93C Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg,d-mannonate and 2-keto-3-deoxy-d- gluconate
32% identity, 100% coverage: 1:382/382 of query aligns to 1:384/384 of 3p93C
- active site: H122 (≠ W119), R147 (≠ K144), Q149 (≠ N146), D192 (= D183), H194 (= H185), E218 (= E209), G243 (= G234), E244 (= E235), R265 (≠ Q256), P267 (≠ D258), H294 (= H285), G295 (≠ C286), E321 (= E310), W384 (= W382)
- binding 2-keto-3-deoxygluconate: N37 (≠ E35), D192 (= D183), H194 (= H185), E244 (= E235), H294 (= H285), P296 (= P287), D298 (vs. gap), E321 (= E310), W384 (= W382)
- binding magnesium ion: D192 (= D183), E218 (= E209), E244 (= E235)
3p93A Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg,d-mannonate and 2-keto-3-deoxy-d- gluconate
32% identity, 100% coverage: 1:382/382 of query aligns to 1:384/384 of 3p93A
- active site: H122 (≠ W119), R147 (≠ K144), Q149 (≠ N146), D192 (= D183), H194 (= H185), E218 (= E209), G243 (= G234), E244 (= E235), R265 (≠ Q256), P267 (≠ D258), H294 (= H285), G295 (≠ C286), E321 (= E310), W384 (= W382)
- binding d-mannonic acid: N37 (≠ E35), D192 (= D183), H194 (= H185), E244 (= E235), H294 (= H285), P296 (= P287), D298 (vs. gap), E321 (= E310)
- binding magnesium ion: D192 (= D183), E218 (= E209), E244 (= E235)
C6D9S0 D-galactonate dehydratase family member PC1_0802; D-mannonate dehydratase; EC 4.2.1.-; EC 4.2.1.8 from Pectobacterium carotovorum subsp. carotovorum (strain PC1) (see paper)
32% identity, 100% coverage: 1:382/382 of query aligns to 1:404/404 of C6D9S0
- D212 (= D183) binding
- E238 (= E209) binding
- E264 (= E235) binding
2ox4C Crystal structure of putative dehydratase from zymomonas mobilis zm4
36% identity, 93% coverage: 1:355/382 of query aligns to 2:381/397 of 2ox4C
- active site: K165 (= K144), D167 (vs. gap), E214 (≠ D183), H216 (= H185), E240 (= E209), G265 (= G234), E266 (= E235), Q287 (= Q256), D289 (= D258), H316 (= H285), E341 (≠ Y317)
- binding magnesium ion: E214 (≠ D183), E240 (= E209), E266 (= E235)
4kwsA Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg and glycerol
32% identity, 100% coverage: 1:382/382 of query aligns to 1:397/397 of 4kwsA
- active site: H122 (≠ W119), R147 (≠ K144), Q149 (≠ N146), Y159 (≠ F153), D205 (= D183), H207 (= H185), E231 (= E209), G256 (= G234), E257 (= E235), R278 (≠ Q256), P280 (≠ D258), H307 (= H285), G308 (≠ C286), E334 (= E310), W397 (= W382)
- binding magnesium ion: D205 (= D183), E231 (= E209), E257 (= E235)
3qkeA Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg and d-gluconate
32% identity, 100% coverage: 1:382/382 of query aligns to 1:397/397 of 3qkeA
- active site: H122 (≠ W119), R147 (≠ K144), Q149 (≠ N146), Y159 (≠ F153), D205 (= D183), H207 (= H185), E231 (= E209), G256 (= G234), E257 (= E235), R278 (≠ Q256), P280 (≠ D258), H307 (= H285), G308 (≠ C286), E334 (= E310), W397 (= W382)
- binding D-gluconic acid: N37 (≠ E35), Y159 (≠ F153), D205 (= D183), H207 (= H185), E257 (= E235), H307 (= H285), P309 (= P287), D311 (vs. gap), E334 (= E310), W397 (= W382)
- binding magnesium ion: D205 (= D183), E231 (= E209), E257 (= E235)
Query Sequence
>AO356_20290 AO356_20290 galactonate dehydratase
MKITKLTTFIVPPRWCFLKVETDEGVTGWGEPVVEGRAHTVAAAVEELSDYLIGKDPRNI
EDIWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGVSVSDLLGGQVRDKIRVYSWI
GGDRPADTARAAKEAVARGFTAVKMNGTEELQFLDTFEKVDLALANVAAVRDAVGPNVGI
GVDFHGRVHKPMAKVLMKELDPYKLMFIEEPVLSENYEALKELAPLTSTPIALGERLFSR
WDFKRVLSEGYVDIIQPDASHAGGITETRKIANMAEAYDVALALHCPLGPIALAACLQLD
AVCYNAFIQEQSLGIHYNESNDLLDYIKDPQVFDYDKGFVKIPNGPGLGIEINEEYVIER
AAIGHRWRNPIWRHADGSFAEW
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory