SitesBLAST

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
25% identity, 78% coverage: 61:406/443 of query aligns to 120:470/582 of Q9JI12

query
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Q9JI12

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H128 (≠ F69) mutation to A: Greatly lowers L-glutamate transport.
R184 (= R123) mutation R->A,E,K: Greatly lowers L-glutamate transport.
E191 (= E130) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
R322 (≠ S260) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.

Sites not aligning to the query:

88 R→A: Impairs synaptic transmission. Abolishes the chloride ion conductance.

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
27% identity, 85% coverage: 56:430/443 of query aligns to 99:476/495 of Q9NRA2

query
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Q9NRA2

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K136 (≠ R93) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ S138) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ AS 153:154) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
IL 266:267 (≠ IE 220:221) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ AGGGL 222:226) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (= G287) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (= P293) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
G371 (= G329) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
25% identity, 78% coverage: 61:406/443 of query aligns to 62:412/452 of 7t3nA

query
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7t3nA

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binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y77 (= Y76), Y137 (≠ F134), Y165 (= Y162), R264 (≠ S260), F268 (≠ L264), Y269 (= Y265)
binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: E152 (= E149), G163 (= G160)

Sites not aligning to the query:

binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: 12, 13

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
29% identity, 80% coverage: 47:399/443 of query aligns to 90:443/495 of Q8BN82

query
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Q8BN82

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P

A

G

G

G

F

Y

I

I

A

A

D

S

R

R

F

V

G

G

S

G

R

K

F

L

T

L

Y

L

G

G

V

L

S

G

L

I

I

L

S

G

W

T

S

S

T

V

V

F

T

T

V

L

C

F

Q

T

G

P

F

L

A

A

T

A

-

D

-

L

G

G

F

V

A

V

S

T

L

L

F

V

G

V

L

L

R

R

L

A

A

L

V

E

G

G

A

L

M

G

E

E

A

G

P

V

A

T

F

F

P

P

A

A

N

M

S
x
H

R

A

A

M

V

W

T

S

V

S

W

W

F

A

P

P

A

P

R

L

E

E

R

R

G

S

L

K

A

L

S

L

S

T

I

I

Y

S

V

Y

C

A

G

G

Q

A

Y

Q

L

L

G

G

T

T

A

V

L

I

F

S

T

L

G

P

A

L

L

S

L

G

W

I

L

I

A

C

T

Y

Y

M

D

N

W

W

R

T

H

Y

V

V

F

F

Y

Y

S

L

T

F

G

G

A

I

L

V

G

G

I

I

V

V

F

W

G

F

V

I

A

L

W

W

L

M

F

W

L

I

Y

V

R

S

D

D

-

T

P

P

M

E

N

T

C

H

K

K

K

T

V

I

S

S

K

H

E

Y

E

E

L

K

K

E

Y

Y

I

I

E

V

A

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G

S

G

L

G

K

L

N

V

Q

K

L

S

S

Q

Q

E

K

R

V

T

V

R

P

F

-

N

-

W

W

R

G

Q

S

I

I

A

L

E

K

L

S

F

L

S

-

Y

-

R

-

Q

P

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L

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W

A

A

I

I

C

V

I

V

G

A

K

H

F

F

A

S

S

Y

T

N

S

W

A

S

L

F

Y

Y

F

T

F

L

L

L

T

T

W

L

F

L

P

P

T

T

Y

Y

L

M

I

K

E

E

E

I

R

L

Q

R

L

F

T

N

M

V

I

Q

K

E

A

N

G

G

I

F

F

L

A

S

V

A

L

L

P

P

F

Y

V

F

G

G

A

C

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W

V

L

G

C

I

M

L

I

L

L

A

C

G

G

I

Q

V

A

S

A

D

D

L

Y

L

L

-

R

I

V

R

K

R

W

G

N

Y

F

S

S

L

T

S

I

F

S

A

V

R

R

K

R

L

I

P

F

L

S

V

L

V

V

G

G

S

-

M

M

L

V

G

G

M

P

S

A

I

V

V

F

L

L

V

V

-

A

N

G

F

F

T

I

D

G

S

C

N

D

V

Y

-

S

I

L

C

A

I

V

A

A

V

F

L

L

T

T

I

I

A

S

F

T

A

L

Q

G

G

G

I

F

A

A

S

S

S

G

W

F

A

S

A

I

V

N

S

H

-

L

E

D

V

I

A

A

P

P

K

S

E

-

L

Y

I

A

G

G

L

I

T

L

G

L

G

G

I

I

T

T

S

N

L

T

A

F

A

A

N

T

I

I

G

P

G

G

I

M

V

T

T

G

P

P

I

I

V

I

H183 (≠ S138) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
27% identity, 83% coverage: 66:434/443 of query aligns to 111:482/497 of Q9Y2C5

query
sites
Q9Y2C5

G

G

L

I

I

I

F

L

S

S

A

S

F

L

A

N

W

Y

T

G

Y

S

A

F

I

L

A

A

L

P

V

I

P

P

A

S

G

G

F

Y

I

V

A

A

D

G

R

I

F

F

G

G

S

A

R

K

F

Y

T

V

Y

V

G

G

V

A

S

G

L

L

I

F

-

I

-

S

S

S

W

F

S

L

T

T

V

L

T

F

V

I

C

P

Q

L

G

A

F

A

A

N

T

A

G

G

F

V

A

A

S

L

L

L

F

I

G

V

L

L

R

R

L

I

A

V

V

Q

G

G

A

I

M

A

E

Q

A

V

P

M

A

V

F

L

P

T

A

G

N

Q

S

Y

R

S

A

I

V

W

T

V

V

K

W

W

F

A

P

P

A

P

R

L

E

E

R

R

G

S

L

Q

A

L

S

T

I

I

Y

A

V

G

C

S

G

G

Q

S

Y

M

L

L

G

G

T

S

-

F

-

I

A

V

L

L

F

L

T

A

G

G

A

G

L

L

W

-

L

-

A

C

T

Q

T

T

Y

I

D

G

W

W

R

P

H

Y

V

V

F

F

Y

Y

S

I

T

F

G

G

A

G

L

I

G

G

I

C

V

A

F

C

G

C

V

P

A

L

W

W

L

F

-

P

F

L

L

I

Y

Y

R

D

D

D

P

P

M

V

N

N

C

H

K

P

K

F

V

I

S

S

K

A

E

G

E

E

L

K

K

R

Y

Y

I

I

E

-

A

-

G

-

L

V

V

C

K

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S

L

S

A

Q

Q

E

Q

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D

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A

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A

A

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A

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F

T

P

P

T

T

Y

Y

L

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Q

L

A

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A

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L

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P

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F

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A

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A

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D

L

F

L

L

I

L

-

S

R

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R

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G

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Y

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L

S

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T

A

I

R

R

K

K

L

L

P

F

L

T

V
x
A

V

I

G

G

S

-

M

V

L

L

G

F

M

P

S

S

I

V

V

I

L

L

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V

N

S

F

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A

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M

T

T

I

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A

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L

A

S

Q

S

G

A

I

I

A

S

S

S

-

F

-

C

S

E

S

S

W

G

A

A

A

L

V

V

S

N

-

F

-

L

E

D

V

I

A

A

P

P

K

R

E

-

L

Y

I

T

G

G

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T

L

G

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G

G

I

L

T

L

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Q

L

V

A

F

A

A

N

H

I

I

G

A

G

G

I

A

V

I

T

S

P

P

I

T

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A

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G

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K

Q

T

D

G

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F

F

A

G

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W

A

R

F

N

W

V

F

F

I

L

G

L

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V

A

A

A

L

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-

N

I

I

G

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T

G

L

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Y

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Y

L

L

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L

F

G

G

R

R

A372 (≠ V327) to T: in dbSNP:rs11754288

Sites not aligning to the query:

66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
28% identity, 78% coverage: 56:399/443 of query aligns to 99:443/495 of Q5Q0U0

query
sites
Q5Q0U0

K

K

D

K

L

Y

G

Q

L

W

D

D

K

A

I

E

E

T

M

Q

G

G

L

W

I

I

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L

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G

A

S

F

F

A

F

W

Y

T

G

Y

Y

A

I

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A

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L

Q

V

I

P

P

A

G

G

G

F

Y

I

M

A

A

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S

R

R

F

I

G

G

S

G

R
x
K

F

L

T

L

Y

L

G

G

V

L

S

G

L

I

I

L

S

G

W

T

S

A

T

V

V

F

T

T

V

L

C

F

Q

T

G

P

F

L

A

A

T

A

-

D

-

L

G

G

F

V

A

V

S

A

L

L

F

I

G

V

L

L

R
|
R

L

A

A

L

V
x
E

G
|
G

A

L

M

G

E
|
E

A
x
G

P

V

A

T

F
|
F

P
|
P

A

A

N

M

S
x
H

R

A

A

M

V
x
W

T

S

V

S

W

W

F

A

P

P

A

P

R

L

E

E

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R

G

S

L

K

A

L

S

L

S

T

I

I

Y

S

V

Y

C

A

G

G

Q

A

Y

Q

L

L

G

G

T

T

A

V

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F

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T

L

G

P

A

L

L

S

L

G

W

I

L

I

A

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T

Y

Y

M

D

N

W

W

R

T

H

Y

V

V

F

F

Y

Y

S

L

T

F

G

G

A

L

L

V

G

G

I

I

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V

F

W

G

F

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I

A

L

W

W

L

M

F

W

L

I

Y

V

R

S

D

D

-

T

P

P

M

E

N

T

C

H

K

K

K

T

V

I

S

S

K

H

E

Y

E

E

L

K

K

E

Y

Y

I

I

E

V

A
x
S

G
x
S

G
x
L

G
x
K

L
x
N

V

Q

K

L

S

S

Q

Q

E

K

R

V

T

V

R

P

F

-

N

-

W

W

R

V

Q

S

I

I

A

L

E

K

L

S

F

L

S

P

Y

-

R

-

Q

-

V

L

W

W

A

A

I

I

C

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I

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G

A

K

H

F

F

A

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S

Y

T

N

S

W

A

T

L

F

Y

Y

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F

L

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T

W

L

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L

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T

Y

Y

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R

L

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M

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L

A

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A

L

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P
|
P

F

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W

V

L

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C

I

M

L

I

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A

S

A

D

D

L

Y

L

L

-

R

I

V

R

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R

W

G

N

Y

F

S

S

L

T

S

I

F

S

A

V

R

R

K

R

L

I

P

F

L

S

V

L

V

I

G
|
G

S

-

M

M

L

V

G

G

M

P

S

A

I

V

V

F

L

L

V

V

-

A

N

G

F

F

T

I

D

G

S

C

N

D

V

Y

-

S

I

L

C

A

I

V

A

A

V

F

L

L

T

T

I

I

A

S

F

T

A

L

Q

G

G

G

I

F

A

A

S

S

S

G

W

F

A

S

A

I

V

N

S

H

-

L

E

D

V

I

A

A

P

P

K

S

E

-

L

Y

I

A

G

G

L

I

T

L

G

L

G

G

I

I

T

T

S

N

L

T

A

F

A

A

N

T

I

I

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G

G

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M

V

I

T

G

P

P

I

I

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I

K136 (≠ R93) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
R168 (= R123) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ V126) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (= G127) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E130) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ A131) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (= F134) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P135) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ S138) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ V141) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSLKN 268:272 (≠ AGGGL 222:226) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
P334 (= P293) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
G371 (= G329) mutation to V: Remains in the endoplasmic reticulum.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.

Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
23% identity, 93% coverage: 18:430/443 of query aligns to 8:459/493 of Q03567

query
sites
Q03567

P

P

E

R

R

L

L

V

P

P

S

S

R

T

R

R

R

-

W

-

F

F

M

A

L

L

S

S

L

L

L

V

L

M

I

F

-

F

A

G

T

C

I

L

I

V

N

T

Y

Y

I

M

D

M

R

R

V

T

N

N

I

M

S

S

I

F

A

A

A

V

P

V

F

C

L

M

A

V

K

N

-

E

-

N

-

K

-

T

D

D

L

T

G

G

L

V

D

E

K

K

I

V

-

S

-

R

-

C

-

G

-

K

E

E

M

M

-

T

-

P

-

V

-

E

-

S

-
x
N

-

S

-

V

-

I

-

G

-

E

-

F

-

D

-

W

-

D

-

K

-

Q

-

T

G

G

L

M

I

V

F

L

S

S

A

S

F

F

A

F

W

Y

T

G

Y

Y

A

I

I

G

A

S

L

Q

V

I

P

I

A

G

G

G

F

H

I

L

A

A

D

S

R

R

F

Y

G

G

S

G

R

K

F

R

T

V

Y

V

G

F

V

V

S

T

L

I

I

L

S

G

W

S

S

A

T

L

V

L

T

T

V

L

C

L

Q

N

G

P

F

V

A

A

-

A

-

R

T

T

G

S

F

E

A

Y

S

A

L

L

F

A

G

I

L

L

R

R

L

A

A

A

V

I

G

G

A

F

M

L

E

Q

A

G

P

A

A

T

F

F

P

P

A

A

N

M

S

H

R

T

A

M

V

W

T

S

V

V

W

W

F

G

P

P

A

P

R

L

E

E

R

L

G

S

L

V

A

L

S

T

S

G

I

V

Y

T

V

Y

C

A

G

G

Q

A

Y

Q

L

I

G

G

T

N

A

V

L

I

-

V

-

L

-

P

F

L

T

S

G

G

A

F

L

L

L

C

W

E

L

Y

A

G

T

F

T

D

Y

G

D

G

W

W

R

P

H

S

V

I

F

F

Y

Y

S

I

T

I

G

G

A

V

L

F

G

G

I

V

V

L

F

W

G

T

V

A

A

V

W

W

L

W

F

Y

L

V

Y

S

R

S

D

D

-

K

P

P

M

A

N

T

C

H

K

P

K

R

V

I

S

T

K

P

E

E

L

K

K

Q

Y

Y

I

I

-

V

-

T

-

A

-

V

E

E

A

A

G

S

G

M

G

G

L

-

V

-

K

K

S

D

S

T

Q

G

E

K

R

V

T

P

R

S

F

T

N

P

W

W

R

I

Q

K

I

I

A

-

E

-

L

-

F

L

S

T

Y

S

R

P

Q

A

V

V

W

W

A

A

I

C

C

W

I

A

G

G

K

H

F

F

A

A

S

G

T

D

S

W

A

G

L

A

Y

Y

F

T

F

M

L

L

T

V

W

S

F

L

P

P

T

S

Y

F

L

L

I

K

E

D

E

V

R

L

Q

G

L

L

T

N

M

L

I

S

K

S

A

L

G

G

I

A

F

V

A

A

V

S

L

I

P

P

F

Y

V

I

G

A

A

Y

T

F

V

L

G

A

I

I

L

N

L

A

A

G

G

G

I

V

V

L

S

A

D

D

L

T

L

L

I

R

R

S

R

K

G

G

Y

I

S

L

L

S

S

T

F

L

A

N

R

T

K

R

L

R

P

A

L

A

V

M

V

L

G

V

S

A

M

L

L

I

G

G

M

Q

S

G

I

I

V

F

L

L

V

V

N

A

F

S

-

G

-

Y

-

C

-

G

T

C

D

G

S

Q

N

D

V

V

I

L

C

V

I

I

A

I

V

F

L

I

T

T

I

C

A

G

F

M

F

A

A

I

Q

S

G

G

I

L

A

Q

S

Y

S

A

S

G

W

F

A

V

-

N

V

Y

S

L

E

E

V

I

A

A

P

P

K

P

E

-

L

F

I

S

G

G

L

T

T

V

G

M

G

G

I

T

T

G

S

N

L

T

A

I

A

S

N

A

I

L

G

A

G

G

I

I

V

I

T

S

P

P

I

A

V

V

I

S

G

S

A

Y

I

L

V

T

H

P

K

N

-

G

-

T

T

Q

G

E

S

E

F

W

A

Q

L

M

A

V

F

L

W

W

F

L

I

T

G

A

G

G

V

I

A

L

L

T

I

I

G

G

T

A

L

L

S

L

Y

F

S

S

L

I

N69 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine

Q9GQQ0 Protein spinster; Protein benchwarmer; Protein diphthong from Drosophila melanogaster (Fruit fly) (see paper)
27% identity, 46% coverage: 15:218/443 of query aligns to 100:305/605 of Q9GQQ0

query
sites
Q9GQQ0

A

A

V

V

P

P

E

S

R

R

L

L

P

S

S

S

-

V

-

G

R

R

R

S

R

Q

W

W

F

F

M

T

L

V

S

T

L

V

L

L

L

C

I

F

A

V

T

N

I

L

I

I

N

N

Y

Y

I

M

D

D

R

R

V

F

N

T

I

I

S

A

I

G

A

V

A

L

P

T

F

D

L

V

A

R

K

N

D

D

L

F

G

D

L

I

D

G

K

N

I

D

E

S

M

A

G

G

L

L

I

L

F

Q

S

T

A

V

F

F

A

V

W

I

T

S

Y

Y

A

M

I

V

A

C

L

A

V

P

P

I

A

F

G

G

F

Y

I

L

A

G

D

D

R

R

F

Y

G

S

S

R

R

P

F

W

T

I

Y

M

G

A

V

V

S

G

L

V

I

G

S

L

W

W

S

S

T

T

V

T

T

T

V

L

C

L

Q

G

G

S

F

F

A

M

T

K

G

Q

F

F

A

G

S

W

L

F

F

I

G

A

L

F

R

R

L

A

A

L

V

V

G

G

A

I

M

G

E
|
E

A

A

P

S

A

Y

F

S

P

T

A

I

N

A

S

P

R

T

A

I

V

I

T

S

V

D

W

L

F

F

P

V

A

H

R

D

E

M

R

R

G

S

L

K

A

M

S

L

S

A

I

L

Y

F

V

Y

C

F

G

A

Q

I

Y

P

L

V

G

G

T

S

A

G

L

L

F

-

T

-

G

G

A

Y

L

I

L

V

W

G

L

S

A

K

T

T

T

A

Y

H

-

L

-

A

-

N

D

D

W

W

R

R

H

W

V

A

F

L

Y

R

S

V

T

T

G

P

A

I

L

L

G

G

I

I

V

V

F

-

G

A

V

V

A

F

W

L

L

I

F

L

L

L

Y

I

R

K

D

D

P

P

M

V

N

R

C

G

K

H

K

S

V

E

S

G

K

S

E

H

E

N

L

L

K

E

E217 (= E130) mutation to K: In bnch(N); leads to storage in yolk spheres during oogenesis and results in widespread accumulation of enlarged lysosomal and late endosomal inclusions.

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
22% identity, 64% coverage: 25:309/443 of query aligns to 19:314/452 of Q5EXK5

query
sites
Q5EXK5

R

R

R

Q

W

W

F

R

M

V

L

I

S

I

L

C

L

F

I

L

A

V

T

V

I

M

I

L

N

D

Y

G

I

F

D

D

R

T

V

A

N

A

I

I

S

G

I

F

A

I

A

A

P

P

F

D

L

I

A

R

K

S

D

H

L

W

G

Q

L

L

D

T

K

A

I

G

E

D

M

L

G

A

L

P

I

L

F

F

S

G

A

A

F

G

A

L

W

L

T

G

Y

L

A

T

I

A

A

G

L

A

V

L

P

L

A

C

G

G

F

P

I

L

A

S

D
|
D

R

R

F

F

G

G

S

R

R

K

F

R

T

V

Y

I

G

E

V

F

S

C

L

V

I

A

S

L

W

F

S

G

T

L

V

F

T

S

V

L

C

L

Q

S

G

A

F

F

A

A

T

P

G

D

F

L

A

Q

S

M

L

L

F

V

G

F

L

L

R

R

L

F

A

L

V

T

G

G

A

L

M

G

E

L

A

G

P

G

A

A

F

M

P

P

A

N

N

T

S

I

R

T

A

M

V

T

T

S

V

E

W

Y

F

L

P

P

A

A

R

R

E

R

R

R

G

G

L

A

A

L

S

V

S

T

I

L

Y

M

V

F

C

C

G

G

Q

F

Y

T

L

L

G

G

T

S

A

A

L

F

F

G

T

G

G

V

A

V

L

S

L

A

W

Q

L

L

A

V

T

P

T

L

Y

I

D

G

W

W

R

H

H

G

V

I

F

L

Y

V

S

L

T

G

G

G

A

V

L

L

G

P

I

L

V

I

F

L

G

A

V

V

A

A

W

L

L

V

-

P

F

L

L

L

Y

P

R

E

D

S

P

P

M

R

N

W

C

Q

K

I

K

R

V

R

S

Q

K

L

E

P

E

Q

L

A

K

T

Y

I

I

A

E

R

A

T

G

V

G

G

G

A

L

I

V

T

K

G

S

E

S

R

Q

Y

E

N

R

D

T

T

R

H

F

F

N

-

W

W

R

L

Q

D

-

E

-

P

-

A

-

G

-

A

-

K

-

G

-

S

I

I

A

S

E

Q

L

L

F

F

S

A

Y

G

R

R

Q

Q

-

L

-

A

-

I

-

T

-

L

-

M

V

L

W

W

A

V

I

V

C

F

I

-

G

-

K

-

F

F

A

M

S

S

T

L

S

L

A

I

L

I

Y

Y

F

L

L

S

T

S

W

W

F

M

P

P

T

T

Y

-

L

L

I

L

E

N

E

H

R

R

Q

G

L

I

T

D

M

L

I

Q

K

H

A

A

G

S

I

W

F

V

A

T

V

A

L

A

P

F

F

Q

V

I

G

G

A

G

T

T

V

F

G

G

I

A

L

L

A

L

G

G

I
x
V

V

L

S

M

D
|
D

D82 (= D88) mutation to A: Loss of activity.
V311 (≠ I306) mutation to W: Loss of activity.
D314 (= D309) mutation to A: Loss of activity.

Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
22% identity, 37% coverage: 3:166/443 of query aligns to 2:167/448 of Q51955

query
sites
Q51955

N

N

S

Q

H

A

T

Q

S

N

Q

S

I

V

A

G

S

K

A

S

R

L

D

D

-

V

-

Q

A

S

V

F

V

I

P

N

E

Q

R

Q

L

P

P

L

S

S

R

R

R

Y

R

Q

W

W

F

R

M

V

L

V

S

L

L

L

L

C

L

F

I

L

A

I

T

V

I

F

I

L

N
x
D

Y

G

I

L

D
|
D

R

T

V

A

N

A

I

M

S

G

I

F

A

I

A

A

P

P

F

A

L

L

A

S

K

Q

D

E

L

W

G

G

L

I

D

D

K

R

I

A

E

S

M

L

G

G

L

P

I

V

F

M

S

S

A

A

F

A

A

L

W

I

T

G

Y

M

A

V

I

F

A

G

L

A

V

L

P

G

A

S

G
|
G

F

P

I

L

A

A

D
|
D

R

R

F

F

G
|
G

S

R

R

K

F

G

T

V

Y

L

G

V

V

G

S

A

L

V

I

L

S

V

W

F

S

G

T

G

V

F

T

S

V

L

C

A

Q

S

G

A

F

Y

A

A

T

T

G

N

F

V

A

D

S

Q

L

L

F

L

G

V

L

L

R
|
R

L

F

A

L

V

T

G

G

A

L

M

G

E

L

A

G

P

A

A

G

F

M

P

P

A

N

N

A

S

T

R

T

A

L

V

L

T

S

V
x
E

W

Y

F

T

P

P

A

E

R

R

E

L

R

K

G

S

L

L

A

L

S

V

S

T

I

S

Y

M

V

F

C

C

G

G

Q

F

Y

N

L

L

G

G

T

M

A

A

D41 (≠ N40) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
D44 (= D43) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
G85 (= G84) mutation to V: Abolishes 4-HBA transport and chemotaxis.
D89 (= D88) mutation to N: Abolishes 4-HBA transport and chemotaxis.
G92 (= G91) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
R124 (= R123) mutation to A: Abolishes 4-HBA transport.
E144 (≠ V143) mutation to A: Strong decrease in 4-HBA transport.

Sites not aligning to the query:

183 H→A: Decrease in 4-HBA transport and chemotaxis.
323 D→N: Abolishes 4-HBA transport and chemotaxis.
328 H→A: Decrease in 4-HBA transport and chemotaxis.; H→R: Decrease in 4-HBA transport and loss of chemotaxis.
386 R→A: Strong decrease in 4-HBA transport.
398 R→A: Abolishes 4-HBA transport.
444 H→A: No change in 4-HBA transport and chemotaxis.

P53322 High-affinity nicotinic acid transporter; Nicotinic acid permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
19% identity, 76% coverage: 2:336/443 of query aligns to 50:395/534 of P53322

query
sites
P53322

S

S

N

H

S

D

H

K

T

K

S

S

Q

E

I

L

A

A

S

T

A

E

R

R

-

E

-

I

-

M

-

A

-

T

-

D

D

D

A

G

V

I

V

P

P

S

E

P

R

S

L

H

P

P

S

M

R

E

R

K

R

R

-

V

-

L

-

R

-

K

-

M

-

D

W

I

F

Y

M

L

L

I

S

P

L

L

L

M

L

G

I

M

A

L

T

Y

I

F

I

L

N

S

Y

N

I

L

D

D

R

K

V

S

N

N

I

I

S

G

I

N

A

A

A

E

-

V

P

A

F

G

L

L

A

S

K

K

D

D

L

I

G

H

L

L

D

V

K

G

I

T

E

Q

M

Y

G

N

L

T

I

C

F

V

S

T

A

V

F

F

A

F

W

A

T

T

Y

Y

A

V

I

L

A

F

L

D

V

P

P

I

A

G

G

T

F

N

I

L

A

L

D

K

R

I

F

M

G

G

S

P

R

P

F

L

T

M

Y

M

G

S

V

I

S

C

L

L

I

T

S

C

W

F

S

G

T

A

V

I

T

S

V

L

C

G

Q

T

G

A

F

W

A

V

T

K

G

N

F

Y

A

A

S

Q

L

L

F

I

G

V

L

V

R

R

L

L

A

L

V

L

G

G

A

A

M

F

E

E

A

G

P

M

A

I

F

Y

P

P

A

A

N

I

S

N

R

M

A

Y

V

L

T

S

V

V

W

C

F

Y

P

R

A

R

R

E

E

Q

R

Y

G

A

L

L

A

R

S

F

S

A

I

F

Y

V

V

F

C

S

G

A

Q

A

Y

C

L

L

G

S

T

S

A

S

L

-

F

F

T

G

G

G

A

L

L

I

L

A

W

Y

L

G

A

C

T

S

T

K

-

I

-

S

-

G

-

S

-

L

Y

K

D

D

W

W

R

Q

H

Y

V

I

F

Y

Y

I

S

V

T

E

G

G

A

C

L

I

G

S

I

L

V

G

F

F

G

V

V

P

A

F

W

Y

L

A

F

F

-

G

L

L

Y

S

R

K

D

N

P

L

M

E

N

D

C

S

K

W

K

F

V

F

S

N

K
|
K

E

E

L

K

K

E

Y

Y

I

I

E

S

A

E

G

R

G

Y

G

K

L

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K283 (= K214) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

P77589 3-(3-hydroxy-phenyl)propionate transporter; 3HPP transporter; 3-(3-hydroxy-phenyl)propionate:H(+) symporter; 3HPP:H(+) symporter from Escherichia coli (strain K12) (see paper)
24% identity, 86% coverage: 60:439/443 of query aligns to 47:398/403 of P77589

query
sites
P77589

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Q

D75 (= D88) mutation D->A,E: Lack of 3HPP transport activity.
A272 (≠ I306) mutation to H: 30% increase in 3HPP transport activity.
K276 (≠ L310) mutation to D: Lack of 3HPP transport activity.

Sites not aligning to the query:

27 E→A: Lack of 3HPP transport activity.; E→D: Slight decrease in 3HPP transport activity.

Q8IVW8 Sphingosine-1-phosphate transporter SPNS2; Protein spinster homolog 2 from Homo sapiens (Human) (see 2 papers)
23% identity, 79% coverage: 24:372/443 of query aligns to 99:446/549 of Q8IVW8

query
sites
Q8IVW8

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R200 (= R123) mutation to S: Loss of function; does not rescue the cardia bifida phenotype in the morpholino knockdown in zebrafish.
S319 (≠ C254) natural variant: Missing (in DFNB115; uncertain significance; dbSNP:rs749994718)

Query Sequence

>AO356_20660 FitnessBrowser__pseudo5_N2C3_1:AO356_20660
MSNSHTSQIASARDAVVPERLPSRRRWFMLSLLLIATIINYIDRVNISIAAPFLAKDLGL
DKIEMGLIFSAFAWTYAIALVPAGFIADRFGSRFTYGVSLISWSTVTVCQGFATGFASLF
GLRLAVGAMEAPAFPANSRAVTVWFPARERGLASSIYVCGQYLGTALFTGALLWLATTYD
WRHVFYSTGALGIVFGVAWLFLYRDPMNCKKVSKEELKYIEAGGGLVKSSQERTRFNWRQ
IAELFSYRQVWAICIGKFASTSALYFFLTWFPTYLIEERQLTMIKAGIFAVLPFVGATVG
ILLAGIVSDLLIRRGYSLSFARKLPLVVGSMLGMSIVLVNFTDSNVICIAVLTIAFFAQG
IASSSWAAVSEVAPKELIGLTGGITSLAANIGGIVTPIVIGAIVHKTGSFALAFWFIGGV
ALIGTLSYSLLLGRLYRIELKAR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory