SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO356_22475 FitnessBrowser__pseudo5_N2C3_1:AO356_22475 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
27% identity, 42% coverage: 38:226/447 of query aligns to 54:222/444 of Q8NLB7

query
sites
Q8NLB7

E
x
D

T

G

Y

Y

D
|
D

L

L

Y

I

L

V

Y

Y

G

G

T

T

A

V

A

Q

A

S

L

A

I

L

F

A

A

K

P

E

L

W

F

-

F

-

P

-

G

-

T

-

D

N

E

L

A

S

V

S

S

A

R

T

L

L

A

G

S

T

L

I

A

G

S

S

F

T

A

A

I

-

S

-

F

F

V

F

A

G

R

M

P

A

L

I

G

G

S

A

L

V

V

F

L

I

G

G

H

R

F

L

G

S

D

D

R
|
R

I

V

G

G

R

R

K

K

K

A

L

A

L

V

Y

I

L

G

T

S

L

V

I

L

I

I

M

L

G

S

L

V

S

F

T

T

V

M

G

L

I

C

G

A

L

F

L

A

P

P

T

N

Y

-

A

-

S

-

V

-

G

-

I

-

W

-

A

-

P

P

I

W

M

V

L

F

C

G

V

A

L

F

R

R

F

F

I

I

Q

A

G

G

F

L

A

G

F

L

A

G

G

G

E

L

Y

V

S

P

G

S

A

V

V

N

L

A

M

M

L

T

L

S

E

D

H

L

A

V

P

P

R

R

R

K

K

T

R

M

G

S

F

A

Y

W

A

A

A

T

I

V

N

M

N

M

I

S

G

G

-

V

P

P

V

I

F

G

G

G

F

S

I

I

A

A

S

A

A

-

G

-

L

-

L

-

L

-

I

-

V

V

S

L

S

A

L

L

L

V

S

V

V

V

E

P

D

S

F

S

Y

E

K

E

W

W

G

G

W

W

R

R

I

F

P

M

F

F

I

L

A

I

S

A

L

L

A

I

L

P

L

L

V

V

V

V

G

G

V

L

F

P

V

I

R

A

S

M

K

K

V

V

A

I

E

P

S

S

D54 (≠ E38) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
D57 (= D41) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (= R94) mutation to A: Loss of transport activity.

Sites not aligning to the query:

309 W→V: Loss of transport activity.
312 D→A: Loss of transport activity.
313 R→A: Loss of transport activity.
317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

Query Sequence

>AO356_22475 FitnessBrowser__pseudo5_N2C3_1:AO356_22475
MQTSLLSNNETHHEFIDDEKKKSMKNVVAGSLFGTALETYDLYLYGTAAALIFAPLFFPG
TDEAVSRLASLASFAISFVARPLGSLVLGHFGDRIGRKKLLYLTLIIMGLSTVGIGLLPT
YASVGIWAPIMLCVLRFIQGFAFAGEYSGAVLMLLEHAPRRKRGFYAAINNIGPVFGFIA
SAGLLLIVSSLLSVEDFYKWGWRIPFIASLALLVVGVFVRSKVAESPVFEKTAEKRAAAK
GPNLSPAMRLFTKYPKQLLLVAGANICHFSTFYLFTVFALSYGQKELGLSNAFVLSVAMV
AICTHLVIVPFAGAMADRLGRRTMMLIGFVVTALAAFPFWHLFSTGQFLPMVLGSCLFMA
GYGLVYGAVPSFTGEAFGPSARFTGFAMATNVGGIIGGGTAPIVGAYLLSHYGSPYAISV
YTVVLAAISALCVYLSAETRMIDITDE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory