SitesBLAST
Comparing AO356_23205 AO356_23205 D-ribose transporter ATP-binding protein to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P04983 Ribose import ATP-binding protein RbsA; EC 7.5.2.7 from Escherichia coli (strain K12) (see paper)
49% identity, 95% coverage: 26:515/517 of query aligns to 4:493/501 of P04983
- K43 (= K65) mutation to R: Loss of transport.
4u00A Crystal structure of ttha1159 in complex with adp (see paper)
30% identity, 42% coverage: 26:242/517 of query aligns to 2:214/241 of 4u00A
3c4jA Abc protein artp in complex with atp-gamma-s
28% identity, 42% coverage: 26:242/517 of query aligns to 3:216/242 of 3c4jA
- binding phosphothiophosphoric acid-adenylate ester: F13 (= F36), V18 (≠ A41), P37 (≠ E60), S38 (≠ N61), G39 (= G62), S40 (≠ A63), G41 (= G64), K42 (= K65), S43 (= S66), T44 (= T67), H133 (≠ E158), Y135 (≠ Q160), E164 (= E189)
3c41J Abc protein artp in complex with amp-pnp/mg2+
28% identity, 42% coverage: 26:242/517 of query aligns to 3:216/242 of 3c41J
- binding phosphoaminophosphonic acid-adenylate ester: F13 (= F36), V18 (≠ A41), P37 (≠ E60), S38 (≠ N61), G39 (= G62), S40 (≠ A63), G41 (= G64), K42 (= K65), S43 (= S66), T44 (= T67), R47 (≠ K70), H133 (≠ E158), Y135 (≠ Q160)
- binding magnesium ion: S43 (= S66), D163 (= D188)
2olkA Abc protein artp in complex with adp-beta-s
28% identity, 42% coverage: 26:242/517 of query aligns to 3:216/242 of 2olkA
2oljA Abc protein artp in complex with adp/mg2+
28% identity, 42% coverage: 26:242/517 of query aligns to 3:216/242 of 2oljA
- binding adenosine-5'-diphosphate: F13 (= F36), V18 (≠ A41), S38 (≠ N61), G39 (= G62), S40 (≠ A63), G41 (= G64), K42 (= K65), S43 (= S66), T44 (= T67), H133 (≠ E158), Y135 (≠ Q160)
4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
27% identity, 41% coverage: 29:242/517 of query aligns to 4:215/240 of 4ymuJ
- binding adenosine-5'-triphosphate: F11 (= F36), L14 (≠ V39), V16 (≠ A41), S36 (≠ N61), G37 (= G62), S38 (≠ A63), G39 (= G64), K40 (= K65), S41 (= S66), T42 (= T67), E51 (≠ Y76), E162 (= E189), H194 (= H221)
- binding magnesium ion: S41 (= S66), E162 (= E189)
1g6hA Crystal structure of the adp conformation of mj1267, an atp- binding cassette of an abc transporter (see paper)
28% identity, 43% coverage: 26:248/517 of query aligns to 4:235/254 of 1g6hA
1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
28% identity, 43% coverage: 26:248/517 of query aligns to 4:235/253 of 1g9xB
3d31A Modbc from methanosarcina acetivorans (see paper)
30% identity, 42% coverage: 26:242/517 of query aligns to 1:207/348 of 3d31A
Sites not aligning to the query:
3fvqB Crystal structure of the nucleotide binding domain fbpc complexed with atp (see paper)
29% identity, 41% coverage: 27:239/517 of query aligns to 4:214/350 of 3fvqB
- binding adenosine-5'-triphosphate: F13 (= F36), Q14 (≠ P37), T16 (≠ V39), V18 (≠ A41), S38 (≠ N61), G39 (= G62), C40 (≠ A63), G41 (= G64), K42 (= K65), T43 (≠ S66), T44 (= T67), Q86 (= Q108), R133 (= R169), E137 (= E173), S139 (≠ A175), G140 (≠ K176), G141 (≠ A177), Q142 (≠ V178)
- binding calcium ion: T43 (≠ S66), Q86 (= Q108)
P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
28% identity, 51% coverage: 31:294/517 of query aligns to 8:268/393 of P9WQI3
- H193 (= H221) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
27% identity, 47% coverage: 27:271/517 of query aligns to 4:245/369 of P19566
- L86 (= L112) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P190) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (≠ T195) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
27% identity, 47% coverage: 27:271/517 of query aligns to 4:245/371 of P68187
- A85 (≠ N111) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ L132) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ T144) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ L147) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (= E149) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ K154) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (≠ A167) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D188) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ S254) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- F241 (≠ L267) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
27% identity, 47% coverage: 27:271/517 of query aligns to 3:244/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F36), V17 (≠ A41), P36 (≠ E60), S37 (≠ N61), G38 (= G62), C39 (≠ A63), G40 (= G64), K41 (= K65), S42 (= S66), T43 (= T67), Q81 (= Q108), R128 (≠ E159), A132 (≠ N163), S134 (= S165), G135 (≠ I166), G136 (≠ A167), Q137 (≠ E168), E158 (= E189), H191 (= H221)
- binding magnesium ion: S42 (= S66), Q81 (= Q108), D157 (= D188)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
27% identity, 47% coverage: 27:271/517 of query aligns to 3:244/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F36), V17 (≠ A41), S37 (≠ N61), G38 (= G62), C39 (≠ A63), G40 (= G64), K41 (= K65), S42 (= S66), T43 (= T67), R128 (≠ E159), A132 (≠ N163), L133 (= L164), S134 (= S165), Q137 (≠ E168)
- binding beryllium trifluoride ion: S37 (≠ N61), G38 (= G62), K41 (= K65), Q81 (= Q108), S134 (= S165), G135 (≠ I166), G136 (≠ A167), E158 (= E189), H191 (= H221)
- binding magnesium ion: S42 (= S66), Q81 (= Q108), D157 (= D188)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
27% identity, 47% coverage: 27:271/517 of query aligns to 3:244/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ F36), V17 (≠ A41), S37 (≠ N61), G38 (= G62), C39 (≠ A63), G40 (= G64), K41 (= K65), S42 (= S66), T43 (= T67), R128 (≠ E159), A132 (≠ N163), S134 (= S165), Q137 (≠ E168)
- binding tetrafluoroaluminate ion: S37 (≠ N61), G38 (= G62), K41 (= K65), Q81 (= Q108), S134 (= S165), G135 (≠ I166), G136 (≠ A167), E158 (= E189), N162 (≠ A193), H191 (= H221)
- binding magnesium ion: S42 (= S66), Q81 (= Q108)
2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
27% identity, 47% coverage: 27:271/517 of query aligns to 3:244/372 of 2awoA