SitesBLAST
Comparing AO356_24170 FitnessBrowser__pseudo5_N2C3_1:AO356_24170 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6tg9A Cryo-em structure of nadh reduced form of NAD+-dependent formate dehydrogenase from rhodobacter capsulatus (see paper)
68% identity, 96% coverage: 12:934/958 of query aligns to 8:938/949 of 6tg9A
- active site: K289 (= K285), C380 (= C376), H381 (= H377), L545 (= L541), G582 (= G578), Q583 (= Q579)
- binding fe2/s2 (inorganic) cluster: C51 (= C56), V59 (≠ F64), G60 (= G65), C62 (= C67), C65 (= C70), C79 (= C84)
- binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: C255 (= C251), K289 (= K285), R351 (= R347), C352 (= C348), C380 (= C376), G414 (= G410), A415 (= A411), D419 (= D415), G420 (≠ A416), H421 (= H417), P443 (= P439), R444 (= R440), P464 (= P460), N467 (= N463), L545 (= L541), G546 (= G542), H550 (= H546), G582 (= G578), Q583 (= Q579), Q583 (= Q579), G649 (= G645), E650 (= E646), S655 (= S651), N680 (= N676), S693 (= S689), K698 (= K694), D724 (= D720), T820 (= T816), T821 (= T817), R823 (= R819), R823 (= R819), I824 (= I820), L825 (= L821), N829 (= N825), V830 (= V826), Q833 (= Q829), N902 (= N898), Y918 (= Y914), K919 (= K915)
- binding iron/sulfur cluster: H111 (= H116), C115 (= C120), C118 (= C123), A120 (= A125), C124 (= C129), C176 (= C171), I177 (= I172), V178 (= V173), C179 (= C174), M180 (≠ S175), C182 (= C177), C186 (= C181), I206 (≠ V201), C218 (= C214), S220 (= S216), C221 (= C217), G222 (= G218), C224 (= C220), C228 (= C224), T230 (= T226), A231 (= A227), C252 (= C248), Y254 (= Y250), C255 (= C251), V257 (= V253), C259 (= C255), F261 (= F257), C287 (= C283), K289 (= K285), V423 (= V419)
7vw6A Cryo-em structure of formate dehydrogenase 1 from methylorubrum extorquens am1 (see paper)
36% identity, 94% coverage: 24:922/958 of query aligns to 2:911/913 of 7vw6A
- binding fe2/s2 (inorganic) cluster: H32 (≠ K54), C34 (= C56), H35 (vs. gap), G45 (= G65), C47 (= C67), R48 (= R68), C50 (= C70), C64 (= C84)
- binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: K263 (= K285), K339 (≠ R347), C364 (= C372), C368 (= C376), G402 (= G410), N404 (= N412), N408 (≠ A416), D431 (= D438), P432 (= P439), R433 (= R440), F447 (≠ L458), G450 (= G461), D452 (≠ N463), G525 (= G540), M526 (≠ L541), G527 (= G542), Q530 (≠ E545), H531 (= H546), G563 (= G578), Q564 (= Q579), G630 (= G645), N632 (≠ D647), S636 (= S651), Q656 (= Q671), D657 (= D672), L658 (≠ I673), T805 (= T817), R807 (= R819), R807 (= R819), V808 (≠ I820), L809 (= L821), H811 (≠ Q823), W812 (≠ Y824), H813 (≠ N825), H813 (≠ N825), T814 (≠ V826), M817 (≠ Q829), F879 (= F890), N887 (= N898), F903 (≠ Y914), K904 (= K915)
- binding iron/sulfur cluster: C145 (= C171), I146 (= I172), Q147 (≠ V173), C148 (= C174), N149 (≠ S175), C151 (= C177), C155 (= C181), N161 (≠ T187), V163 (≠ A189), I164 (≠ L190), V175 (= V201), C188 (= C214), V189 (= V215), A190 (≠ S216), C191 (= C217), G192 (= G218), C194 (= C220), C198 (= C224), P199 (= P225), T200 (= T226), A202 (≠ T228), L203 (= L229), C227 (= C248), C230 (= C251), C234 (= C255), C261 (= C283), K263 (= K285), G264 (= G286), V411 (= V419)
1fdiA Oxidized form of formate dehydrogenase h from e. Coli complexed with the inhibitor nitrite (see paper)
38% identity, 74% coverage: 242:954/958 of query aligns to 2:715/715 of 1fdiA
- active site: C11 (= C251), L41 (≠ S282), C42 (= C283), K44 (= K285), S108 (= S345), R110 (= R347), D134 (= D370), C140 (= C376), H141 (= H377), S180 (≠ A416), M297 (≠ L541), R333 (= R577), G334 (= G578), Q335 (= Q579)
- binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: K44 (= K285), R110 (= R347), G111 (≠ C348), V139 (= V375), C140 (= C376), F173 (≠ M409), G174 (= G410), Y175 (≠ A411), N176 (= N412), D179 (= D415), S180 (≠ A416), C201 (≠ I437), D202 (= D438), P203 (= P439), R204 (= R440), L218 (= L458), G221 (= G461), N223 (= N463), G296 (= G540), M297 (≠ L541), G298 (= G542), F302 (≠ H546), G334 (= G578), Q335 (= Q579), Q335 (= Q579), G402 (= G645), E403 (= E646), T408 (≠ S651), Q428 (= Q671), D429 (= D672), I430 (= I673), S445 (≠ G688), D478 (= D720), T579 (= T817), V580 (≠ G818), R581 (= R819), R581 (= R819), E582 (≠ I820), H585 (≠ Q823), Y586 (= Y824), S587 (≠ N825), C588 (≠ V826), Y654 (≠ F890), N662 (= N898), Y678 (= Y914), K679 (= K915)
- binding nitrite ion: C140 (= C376), H141 (= H377), R333 (= R577), G334 (= G578), V338 (= V582)
- binding iron/sulfur cluster: C8 (= C248), Y10 (= Y250), C11 (= C251), S13 (≠ V253), C15 (= C255), L41 (≠ S282), C42 (= C283), K44 (= K285), P182 (= P418), I183 (≠ V419)
P07658 Formate dehydrogenase H; Formate dehydrogenase-H subunit alpha; FDH-H; Formate-hydrogen-lyase-linked, selenocysteine-containing polypeptide; EC 1.17.98.4 from Escherichia coli (strain K12) (see 2 papers)
38% identity, 74% coverage: 242:954/958 of query aligns to 2:715/715 of P07658
- C8 (= C248) binding [4Fe-4S] cluster
- C11 (= C251) binding [4Fe-4S] cluster
- C15 (= C255) binding [4Fe-4S] cluster
- C42 (= C283) binding [4Fe-4S] cluster
- R110 (= R347) binding Mo-bis(molybdopterin guanine dinucleotide)
- U140 (≠ C376) binding Mo-bis(molybdopterin guanine dinucleotide); modified: nonstandard, Selenocysteine
- N176 (= N412) binding Mo-bis(molybdopterin guanine dinucleotide)
- D179 (= D415) binding Mo-bis(molybdopterin guanine dinucleotide)
- S180 (≠ A416) binding Mo-bis(molybdopterin guanine dinucleotide)
- C201 (≠ I437) binding Mo-bis(molybdopterin guanine dinucleotide)
- D202 (= D438) binding Mo-bis(molybdopterin guanine dinucleotide)
- R204 (= R440) binding Mo-bis(molybdopterin guanine dinucleotide)
- G221 (= G461) binding Mo-bis(molybdopterin guanine dinucleotide)
- N223 (= N463) binding Mo-bis(molybdopterin guanine dinucleotide)
- M297 (≠ L541) binding Mo-bis(molybdopterin guanine dinucleotide)
- Q335 (= Q579) binding Mo-bis(molybdopterin guanine dinucleotide)
- D404 (= D647) binding Mo-bis(molybdopterin guanine dinucleotide)
- T408 (≠ S651) binding Mo-bis(molybdopterin guanine dinucleotide)
- Q428 (= Q671) binding Mo-bis(molybdopterin guanine dinucleotide)
- D429 (= D672) binding Mo-bis(molybdopterin guanine dinucleotide)
- S445 (≠ G688) binding Mo-bis(molybdopterin guanine dinucleotide)
- D478 (= D720) binding Mo-bis(molybdopterin guanine dinucleotide)
- R581 (= R819) binding Mo-bis(molybdopterin guanine dinucleotide)
- E582 (≠ I820) binding Mo-bis(molybdopterin guanine dinucleotide)
- H585 (≠ Q823) binding Mo-bis(molybdopterin guanine dinucleotide)
- S587 (≠ N825) binding Mo-bis(molybdopterin guanine dinucleotide)
- Y678 (= Y914) binding Mo-bis(molybdopterin guanine dinucleotide)
- K679 (= K915) binding Mo-bis(molybdopterin guanine dinucleotide)
2iv2X Reinterpretation of reduced form of formate dehydrogenase h from e. Coli (see paper)
38% identity, 74% coverage: 242:954/958 of query aligns to 2:697/697 of 2iv2X
- active site: C11 (= C251), L41 (≠ S282), C42 (= C283), K44 (= K285), S108 (= S345), C140 (= C376), H141 (= H377), S180 (≠ A416), M297 (≠ L541), R333 (= R577), G334 (= G578), Q335 (= Q579)
- binding guanylate-o'-phosphoric acidmono-(2-amino-5,6-dimercapto-4-oxo-3,5,6,7,8a,9,10,10a-octahydro-4h-8-oxa-1,3,9,10-tetraaza-anthracen-7-ylmethyl) ester: R110 (= R347), G111 (≠ C348), T112 (= T349), A137 (= A373), Q335 (= Q579), G402 (= G645), E403 (= E646), D404 (= D647), T408 (≠ S651), A410 (≠ P653), Q428 (= Q671), D429 (= D672), I430 (= I673), S445 (≠ G688), D478 (= D720), C588 (≠ V826), Y660 (= Y914)
- binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: K44 (= K285), F173 (≠ M409), G174 (= G410), Y175 (≠ A411), N176 (= N412), D179 (= D415), S180 (≠ A416), D202 (= D438), P203 (= P439), R204 (= R440), N223 (= N463), G296 (= G540), M297 (≠ L541), F302 (≠ H546), G334 (= G578), R581 (= R819), E582 (≠ I820), V583 (≠ L821), H585 (≠ Q823), Y586 (= Y824), S587 (≠ N825), K661 (= K915)
- binding iron/sulfur cluster: C8 (= C248), C11 (= C251), S13 (≠ V253), C15 (= C255), L41 (≠ S282), C42 (= C283), K44 (= K285), G45