SitesBLAST
Comparing AO356_27985 AO356_27985 sorbitol dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6pejA Structure of sorbitol dehydrogenase from sinorhizobium meliloti 1021 bound to sorbitol
84% identity, 100% coverage: 1:257/257 of query aligns to 1:257/257 of 6pejA
Q48436 Diacetyl reductase [(S)-acetoin forming]; Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase; EC 1.1.1.304 from Klebsiella pneumoniae (see paper)
37% identity, 96% coverage: 7:253/257 of query aligns to 3:252/256 of Q48436
- 6:33 (vs. 10:37, 57% identical) binding
- D59 (= D60) binding
- K156 (= K157) binding
1gegE Cryatal structure analysis of meso-2,3-butanediol dehydrogenase (see paper)
37% identity, 96% coverage: 7:253/257 of query aligns to 3:252/256 of 1gegE
- active site: G13 (= G17), S139 (= S140), Y152 (= Y153), K156 (= K157), V197 (≠ F198)
- binding alpha-D-glucopyranose: R63 (≠ Q64), D64 (≠ A65), F67 (≠ D68), E123 (≠ R124)
- binding nicotinamide-adenine-dinucleotide: G9 (= G13), Q12 (≠ R16), I14 (= I18), D33 (= D37), Y34 (≠ I38), V58 (≠ M59), D59 (= D60), V60 (= V61), N86 (= N87), A87 (= A88), I109 (= I110), S139 (= S140), Y152 (= Y153), K156 (= K157), P182 (= P183), V185 (= V186), T187 (≠ G188), M189 (≠ H190)
3wyeA Crystal structure of chimeric engineered (2s,3s)-butanediol dehydrogenase complexed with NAD+
37% identity, 96% coverage: 7:253/257 of query aligns to 2:251/255 of 3wyeA
- active site: G12 (= G17), S138 (= S140), Y151 (= Y153), K155 (= K157), L196 (≠ F198)
- binding nicotinamide-adenine-dinucleotide: G8 (= G13), Q11 (≠ R16), G12 (= G17), I13 (= I18), D32 (= D37), Y33 (≠ I38), V57 (≠ M59), D58 (= D60), V59 (= V61), N85 (= N87), A86 (= A88), S138 (= S140), Y151 (= Y153), K155 (= K157), P181 (= P183), G182 (= G184), V184 (= V186), T186 (≠ G188), M188 (≠ H190), W189 (= W191)
Q9ZNN8 L-2,3-butanediol dehydrogenase; L-BDH; (S,S)-butanediol dehydrogenase; Diacetyl reductase [(S)-acetoin forming]; EC 1.1.1.76; EC 1.1.1.304 from Corynebacterium glutamicum (Brevibacterium saccharolyticum) (see paper)
36% identity, 96% coverage: 7:253/257 of query aligns to 3:254/258 of Q9ZNN8
- QGI 12:14 (≠ RGI 16:18) binding
- D33 (= D37) binding
- Q37 (≠ E41) binding
- DV 61:62 (= DV 60:61) binding
- N88 (= N87) binding
- I142 (≠ Q141) mutation to Q: Loss of L-BD oxidizing activity, and does not gain the ability to use meso-BD as substrate; when associated with N-148.; mutation to Q: Loss of L-BD oxidizing activity. Does not gain the ability to use meso-BD as substrate.
- F148 (≠ E147) mutation to N: Loss of L-BD oxidizing activity, and does not gain the ability to use meso-BD as substrate; when associated with Q-142.; mutation to N: Loss of L-BD oxidizing activity. Does not gain the ability to use meso-BD as substrate.
- Y154 (= Y153) binding
- K158 (= K157) binding
- PGIVGT 184:189 (≠ PGVVDG 183:188) binding
3a28C Crystal structure of l-2,3-butanediol dehydrogenase (see paper)
36% identity, 96% coverage: 7:253/257 of query aligns to 2:253/257 of 3a28C
- active site: G12 (= G17), S140 (= S140), Y153 (= Y153), K157 (= K157), L198 (≠ F198)
- binding nicotinamide-adenine-dinucleotide: G8 (= G13), Q11 (≠ R16), I13 (= I18), D32 (= D37), L33 (≠ I38), Q36 (≠ E41), L59 (≠ M59), D60 (= D60), V61 (= V61), N87 (= N87), S140 (= S140), Y153 (= Y153), K157 (= K157), P183 (= P183), V186 (= V186), T188 (≠ G188), M190 (≠ H190), W191 (= W191)
7do7A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NAD and l-rhamnose bound-form) (see paper)
37% identity, 98% coverage: 7:257/257 of query aligns to 6:254/256 of 7do7A
- active site: G16 (= G17), S146 (= S140), Y159 (= Y153)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), R15 (= R16), G16 (= G17), I17 (= I18), S37 (≠ A36), D66 (= D60), A67 (≠ V61), N93 (= N87), A94 (= A88), G95 (≠ A89), I96 (≠ L90), V144 (≠ M138), S145 (≠ A139), S146 (= S140), Y159 (= Y153), K163 (= K157), P189 (= P183), G190 (= G184), I192 (≠ V186), T194 (≠ L197), I196 (≠ A199)
- binding beta-L-rhamnopyranose: F99 (≠ L93), S146 (= S140), S148 (≠ A142), Q156 (≠ V150), Y159 (= Y153), N197 (≠ R200), D235 (≠ A238), M236 (≠ E239), R238 (≠ E241)
7b81A Crystal structure of azotobacter vinelandii l-rhamnose 1-dehydrogenase (NAD bound-form) (see paper)
37% identity, 98% coverage: 7:257/257 of query aligns to 6:254/256 of 7b81A
- active site: G16 (= G17), S146 (= S140), Y159 (= Y153)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), S14 (≠ A15), R15 (= R16), I17 (= I18), D66 (= D60), A67 (≠ V61), N93 (= N87), A94 (= A88), G95 (≠ A89), I96 (≠ L90), T116 (≠ I110), V144 (≠ M138), S146 (= S140), Y159 (= Y153), K163 (= K157), P189 (= P183), G190 (= G184), I192 (≠ V186), T194 (≠ L197), I196 (≠ A199)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
36% identity, 100% coverage: 1:256/257 of query aligns to 2:244/244 of 4nbuB
- active site: G18 (= G17), N111 (= N111), S139 (= S140), Q149 (≠ V150), Y152 (= Y153), K156 (= K157)
- binding acetoacetyl-coenzyme a: D93 (≠ L93), K98 (≠ D98), S139 (= S140), N146 (≠ E147), V147 (≠ A148), Q149 (≠ V150), Y152 (= Y153), F184 (≠ V185), M189 (≠ L197), K200 (≠ Q212)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G13), N17 (≠ R16), G18 (= G17), I19 (= I18), D38 (= D37), F39 (≠ I38), V59 (≠ M59), D60 (= D60), V61 (= V61), N87 (= N87), A88 (= A88), G89 (≠ A89), I90 (≠ L90), T137 (≠ M138), S139 (= S140), Y152 (= Y153), K156 (= K157), P182 (= P183), F184 (≠ V185), T185 (≠ V186), T187 (≠ D195), M189 (≠ L197)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
38% identity, 97% coverage: 7:256/257 of query aligns to 5:246/246 of 3osuA
1zemA Crystal structure of NAD+-bound xylitol dehydrogenase (see paper)
36% identity, 98% coverage: 2:253/257 of query aligns to 1:260/260 of 1zemA
- active site: N16 (≠ G17), S142 (= S140), Y155 (= Y153), K159 (= K157), D212 (≠ K209)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), G15 (≠ R16), N16 (≠ G17), I17 (= I18), D36 (= D37), M37 (≠ I38), D62 (= D60), V63 (= V61), N89 (= N87), A90 (= A88), G91 (≠ A89), T140 (≠ M138), S142 (= S140), Y155 (= Y153), K159 (= K157), P185 (= P183), M188 (≠ V186)
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
37% identity, 97% coverage: 7:256/257 of query aligns to 2:239/239 of 3sj7A
- active site: G12 (= G17), S138 (= S140), Q148 (≠ V150), Y151 (= Y153), K155 (= K157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G13), S10 (≠ A15), R11 (= R16), I13 (= I18), N31 (≠ A36), Y32 (≠ D37), A33 (≠ I38), G34 (vs. gap), S35 (≠ D39), A58 (≠ M59), N59 (≠ D60), V60 (= V61), N86 (= N87), A87 (= A88), T109 (≠ I110), S138 (= S140), Y151 (= Y153), K155 (= K157), P181 (= P183), G182 (= G184)
3ak4A Crystal structure of nadh-dependent quinuclidinone reductase from agrobacterium tumefaciens
34% identity, 97% coverage: 4:253/257 of query aligns to 5:254/258 of 3ak4A
- active site: G18 (= G17), S141 (= S140), L151 (≠ V150), Y154 (= Y153), K158 (= K157), E199 (≠ F198)
- binding nicotinamide-adenine-dinucleotide: K17 (≠ R16), G18 (= G17), I19 (= I18), D38 (= D37), L39 (≠ I38), V60 (≠ M59), D61 (= D60), V62 (= V61), N88 (= N87), A89 (= A88), G90 (≠ A89), T139 (≠ M138), S141 (= S140), Y154 (= Y153), K158 (= K157), G185 (= G184), V187 (= V186), T189 (≠ G188), M191 (≠ H190)
8cxaA Crystal structure of 3-oxoacyl-[acyl-carrier-protein] reductase from mycobacterium smegmatis with bound NAD
35% identity, 98% coverage: 3:253/257 of query aligns to 2:247/251 of 8cxaA
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), Q15 (≠ R16), G16 (= G17), I17 (= I18), D36 (= D37), V63 (= V61), N89 (= N87), A91 (= A89), S94 (≠ D92), I142 (≠ M138), S143 (≠ A139), S144 (= S140), Y157 (= Y153), K161 (= K157), P187 (= P183), H188 (≠ D192), I190 (≠ V194), I194 (≠ F198)
7v0hG Crystal structure of putative glucose 1-dehydrogenase from burkholderia cenocepacia in complex with NADP and a potential reaction product
37% identity, 98% coverage: 1:253/257 of query aligns to 6:251/253 of 7v0hG
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G13), S20 (≠ A15), K21 (≠ R16), G22 (= G17), I23 (= I18), A43 (≠ D39), S44 (≠ L40), S45 (≠ E41), G68 (≠ M59), D69 (= D60), V70 (= V61), N96 (= N87), S97 (≠ A88), G98 (≠ A89), Y100 (≠ F91), I144 (≠ M138), S146 (= S140), Y159 (= Y153), K163 (= K157), P189 (= P183), G190 (= G184), M191 (vs. gap), I192 (vs. gap), T194 (≠ V186), G196 (= G188), T197 (≠ E189)
- binding (2R)-2-(hydroxymethyl)pentanedioic acid: S146 (= S140), Y159 (= Y153), M191 (vs. gap), I202 (≠ V194)
7do6A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NADP bound-form) (see paper)
36% identity, 98% coverage: 7:257/257 of query aligns to 6:245/247 of 7do6A
- active site: G16 (= G17), S146 (= S140), Y159 (= Y153)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), S14 (≠ A15), R15 (= R16), G16 (= G17), I17 (= I18), H36 (vs. gap), S37 (≠ A36), G42 (≠ E41), D66 (= D60), A67 (≠ V61), N93 (= N87), A94 (= A88), G95 (≠ A89), I96 (≠ L90), T116 (≠ I110), S146 (= S140), Y159 (= Y153), K163 (= K157), I192 (≠ V186)
5u9pB Crystal structure of a gluconate 5-dehydrogenase from burkholderia cenocepacia j2315 in complex with NADP and tartrate
36% identity, 98% coverage: 3:253/257 of query aligns to 13:256/261 of 5u9pB
- active site: G27 (= G17), S152 (= S140), Y165 (= Y153), K169 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G23 (= G13), R26 (= R16), G27 (= G17), I28 (= I18), R48 (≠ I38), D73 (= D60), V74 (= V61), N100 (= N87), A101 (= A88), I150 (≠ M138), Y165 (= Y153), K169 (= K157), P195 (= P183), F198 (≠ V186), T200 (≠ G188), L202 (≠ H190), N203 (≠ W191)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
36% identity, 98% coverage: 4:256/257 of query aligns to 3:247/247 of 4jroC
- active site: G16 (= G17), S142 (= S140), Q152 (≠ V150), Y155 (= Y153), K159 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), S14 (≠ A15), R15 (= R16), G16 (= G17), I17 (= I18), N35 (≠ A36), Y36 (vs. gap), N37 (≠ D37), G38 (≠ I38), S39 (≠ D39), N63 (≠ D60), V64 (= V61), N90 (= N87), A91 (= A88), I93 (≠ L90), I113 (= I110), S142 (= S140), Y155 (= Y153), K159 (= K157), P185 (= P183), I188 (≠ V186), T190 (≠ G188)
4za2D Crystal structure of pectobacterium carotovorum 2-keto-3-deoxy-d- gluconate dehydrogenase complexed with NAD+ (see paper)
32% identity, 99% coverage: 4:257/257 of query aligns to 8:246/247 of 4za2D
- binding nicotinamide-adenine-dinucleotide: G17 (= G13), D19 (≠ A15), L22 (≠ I18), I42 (= I38), D65 (= D60), M66 (≠ V61), N92 (= N87), A93 (= A88), G94 (≠ A89), L115 (≠ I110), I143 (≠ M138), S145 (= S140), Y158 (= Y153), K162 (= K157), G189 (= G184), M191 (≠ V186), T193 (≠ E202), N195 (≠ L204)
7krmC Putative fabg bound to nadh from acinetobacter baumannii
33% identity, 97% coverage: 4:253/257 of query aligns to 3:240/244 of 7krmC
- active site: G18 (= G17), S140 (= S140), Y155 (= Y153)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), S15 (vs. gap), G18 (= G17), I19 (= I18), D38 (= D37), L39 (≠ I38), A60 (≠ M59), N61 (≠ D60), V62 (= V61), N88 (= N87), V111 (≠ I110), S140 (= S140), Y155 (= Y153), K159 (= K157), I188 (≠ V186), T190 (≠ G188)
Query Sequence
>AO356_27985 AO356_27985 sorbitol dehydrogenase
MKRLEGKSALVTGAARGIGRAFAQAYIEEGATVAIADIDLERANATAAELGDSAYAVKMD
VTDQASIDQAIAAVVAQAGKLDILINNAALFDLAPIVDITRQSYERLFSINVAGTLFTLQ
AAARQMIRQGHGGRIINMASQAGRRGEALVAVYCATKAAVISLTQSAGLDLIKHRINVNA
IAPGVVDGEHWDGVDALFARHENLPQGEKKRQVGQQVPYGRMGTAQDLTGMAIFLASAES
EYVVAQTYNVDGGNWMS
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory