SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO356_28705 AO356_28705 hypothetical protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5lh9D Amine transaminase crystal structure from an uncultivated pseudomonas species in the plp-bound (internal aldimine) form
89% identity, 100% coverage: 2:448/449 of query aligns to 2:448/449 of 5lh9D

query
sites
5lh9D

Y

Y

E

E

H

K

Y

Y

K

K

T

N

A

A

Q

E

K

K

K

K

F

F

W

W

H

H

P

P

M

M

S

G

S

S

S

S

A

A

P

A

A

P

N

H

R

R

S

D

K

K

T

T

L

L

I

V

I

I

A

A

R

R

G

G

D

D

G

G

N

N

Y

Y

I

I

T

T

D

D

I

I

E

D

G

G

H

Q

R

R

M

M

L

L

D

D

G

G

V

V

G

G

G

G

L

L

W

W

N

N

V

V

N

N

V

I

G

G

H

H

N

N

R

R

P

A

S

S

V

V

K

K

A

A

A

A

I

I

A

A

A

A

Q

Q

L

L

D

D

E

E

L

L

A

A

Y

Y

Y

Y

Q

Q

T

T

F

F

D

D

G

G

I

I

A

A

H

H

P

P

R

R

V

V

F

F

D

D

L

L

A

A

E

E

R

R

L

L

T

T

S

G

M

M

F

F

A

A

Q

Q

E

E

N

R

M

M

A

A

R

R

V

V

L

L

F

F

S

S

S

S

G
|
G

G
|
G

S
|
S

D

D

A

A

V

V

E

E

T

T

A

A

L

L

K

K

M

M

A

A

R

R

Q

Q

Y

Y

W

W

I

I

A

A

S

S

G

G

E

E

P

P

G

G

R

R

T

T

R

R

F

F

L

L

S

S

L

L

R

R

N

N

G

G

Y
|
Y

H
|
H

G
|
G

V

V

H

H

V

M

G

G

G

G

T

T

S

S

V

V

G

G

G

G

N

N

G

G

V

V

Y

Y

H

H

Y

Y

N

N

H

H

G

G

P

Q

L

L

L

L

A

A

G

G

C

C

H

H

L

L

L

L

D

D

T

T

P

P

W

W

L

L

Y

Y

R

R

N

N

P

P

W

W

D

D

C

C

R

R

D

D

P

P

E

Q

E

A

L

L

T

T

A

A

H

H

C

C

I

I

R

R

Q

Q

L

L

E

E

D

E

Q

Q

I

I

A

A

L

L

L

L

G

G

P

A

Q

Q

T

T

I

I

A

A

A

A

L

L

I

I

A

A

E
|
E

P

P

V

V

Q

Q

G

G

A

A

G

G

G

G

V

V

I

I

V

V

P

P

P

P

A

A

H

D

Y

Y

W

W

K

P

R

R

L

L

R

R

E

E

V

V

C

C

D

D

R

R

H

H

G

G

I

I

L

L

L

L

I

I

A

A

D
|
D

E

E

V
|
V

V
|
V

T

T

G

G

F

F

G

G

R

R

T

S

G

G

C

C

M

M

L

L

G

G

S

S

R

R

G

G

W

W

G

G

V

V

A

A

P

P

D

D

V

I

L

L

C

C

L

L

A

A

K
|
K

G

G

I

I

T

T

A

A

G

G

Y

Y

I

I

P

P

M

L

G

G

A

A

T

T

V

L

F

F

N

N

Q

Q

R

R

I

I

A

A

D

D

A

A

I

I

E

E

N

N

G

G

P

Q

G

G

F

F

S

S

S

H

V

M

I

I

M

M

H

H

G

G

Y
|
Y

T
|
T

Y

Y

S

S

G

G

H

H

P

P

T

T

A

A

C

C

A

A

A

A

A

A

L

L

A

A

V

V

L

L

D

D

I

I

V

V

E

E

A

A

E

E

D

D

L

L

P

P

G

G

N

N

A

A

G

A

K

K

V

V

G

G

A

A

Q

Q

L

L

L

L

E

E

Q

Q

L

L

Q

Q

P

P

L

L

T

V

E

E

R

R

Y

Y

A

A

V

V

V

V

G

G

E

E

V

V

R

R

G

G

K

K

G

G

L

L

M

M

I

I

A

A

V

L

D

D

L

L

V

V

A

S

D

D

K

K

V

R

T

T

R

R

E

Q

P

P

L

L

D

D

P

P

A

A

N

A

G

G

L

Q

A

P

S

S

R

R

I

I

A

A

E

D

Q

E

A

A

R

R

R

R

A

A

G

G

V

V

L

L

V

V

R

R

P

P

I

I

G

G

N

N

K

K

I

I

V

I

M

L

S

S

P

P

P

P

L

L

T

T

L

L

T

T

S

R

D

D

E

E

A

A

A

G

M

L

M

M

V

V

G

S

A

A

L

L

D

E

G

A

A

A

L

F

A

A

D

R

C

C

active site: Y148 (= Y148), D255 (= D255), K284 (= K284), T321 (= T321)
binding pyridoxal-5'-phosphate: G114 (= G114), G115 (= G115), S116 (= S116), Y148 (= Y148), H149 (= H149), G150 (= G150), E222 (= E222), D255 (= D255), V257 (= V257), V258 (= V258), K284 (= K284), Y320 (= Y320), T321 (= T321)

5lhaA Amine transaminase crystal structure from an uncultivated pseudomonas species in the pmp-bound form
89% identity, 99% coverage: 3:448/449 of query aligns to 1:446/447 of 5lhaA

query
sites
5lhaA

E

E

H

K

Y

Y

K

K

T

N

A

A

Q

E

K

K

K

K

F

F

W

W

H

H

P

P

M

M

S

G

S

S

S

S

A

A

P

A

A

P

N

H

R

R

S

D

K

K

T

T

L

L

I

V

I

I

A

A

R

R

G

G

D

D

G

G

N

N

Y

Y

I

I

T

T

D

D

I

I

E

D

G

G

H

Q

R

R

M

M

L

L

D

D

G

G

V

V

G

G

G

G

L

L

W

W

N

N

V

V

N

N

V

I

G

G

H

H

N

N

R

R

P

A

S

S

V

V

K

K

A

A

A

A

I

I

A

A

A

A

Q

Q

L

L

D

D

E

E

L

L

A

A

Y

Y

Y

Y

Q

Q

T

T

F

F

D

D

G

G

I

I

A

A

H

H

P

P

R

R

V

V

F

F

D

D

L

L

A

A

E

E

R

R

L

L

T

T

S

G

M

M

F

F

A

A

Q

Q

E

E

N

R

M

M

A

A

R

R

V

V

L

L

F

F

S

S

S

S

G
|
G

G
|
G

S
|
S

D

D

A

A

V

V

E

E

T

T

A

A

L

L

K

K

M

M

A

A

R

R

Q

Q

Y

Y

W

W

I

I

A

A

S

S

G

G

E

E

P

P

G

G

R

R

T

T

R

R

F

F

L

L

S

S

L

L

R

R

N

N

G

G

Y
|
Y

H
|
H

G
|
G

V

V

H

H

V

M

G

G

G

G

T

T

S

S

V

V

G

G

G

G

N

N

G

G

V

V

Y

Y

H

H

Y

Y

N

N

H

H

G

G

P

Q

L

L

L

L

A

A

G

G

C

C

H

H

L

L

L

L

D

D

T

T

P

P

W

W

L

L

Y

Y

R

R

N

N

P

P

W

W

D

D

C

C

R

R

D

D

P

P

E

Q

E

A

L

L

T

T

A

A

H

H

C

C

I

I

R

R

Q

Q

L

L

E

E

D

E

Q

Q

I

I

A

A

L

L

L

L

G

G

P

A

Q

Q

T

T

I

I

A

A

A

A

L

L

I

I

A

A

E
|
E

P

P

V

V

Q

Q

G

G

A

A

G

G

G

G

V

V

I

I

V

V

P

P

P

P

A

A

H

D

Y

Y

W

W

K

P

R

R

L

L

R

R

E

E

V

V

C

C

D

D

R

R

H

H

G

G

I

I

L

L

L

L

I

I

A

A

D
|
D

E

E

V
|
V

V
|
V

T

T

G

G

F

F

G

G

R

R

T

S

G

G

C

C

M

M

L

L

G

G

S

S

R

R

G

G

W

W

G

G

V

V

A

A

P

P

D

D

V

I

L

L

C

C

L

L

A

A

K
|
K

G

G

I

I

T

T

A

A

G

G

Y

Y

I

I

P

P

M

L

G

G

A

A

T

T

V

L

F

F

N

N

Q

Q

R

R

I

I

A

A

D

D

A

A

I

I

E

E

N

N

G

G

P

Q

G

G

F

F

S

S

S

H

V

M

I

I

M

M

H

H

G

G

Y
|
Y

T
|
T

Y

Y

S

S

G

G

H

H

P

P

T

T

A

A

C

C

A

A

A

A

A

A

L

L

A

A

V

V

L

L

D

D

I

I

V

V

E

E

A

A

E

E

D

D

L

L

P

P

G

G

N

N

A

A

G

A

K

K

V

V

G

G

A

A

Q

Q

L

L

L

L

E

E

Q

Q

L

L

Q

Q

P

P

L

L

T

V

E

E

R

R

Y

Y

A

A

V

V

V

V

G

G

E

E

V

V

R

R

G

G

K

K

G

G

L

L

M

M

I

I

A

A

V

L

D

D

L

L

V

V

A

S

D

D

K

K

V

R

T

T

R

R

E

Q

P

P

L

L

D

D

P

P

A

A

N

A

G

G

L

Q

A

P

S

S

R

R

I

I

A

A

E

D

Q

E

A

A

R

R

R

R

A

A

G

G

V

V

L

L

V

V

R

R

P

P

I

I

G

G

N

N

K

K

I

I

V

I

M

L

S

S

P

P

P

P

L

L

T

T

L

L

T

T

S

R

D

D

E

E

A

A

A

G

M

L

M

M

V

V

G

S

A

A

L

L

D

E

G

A

A

A

L

F

A

A

D

R

C

C

active site: Y146 (= Y148), D253 (= D255), K282 (= K284), T319 (= T321)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G112 (= G114), G113 (= G115), S114 (= S116), Y146 (= Y148), H147 (= H149), G148 (= G150), E220 (= E222), D253 (= D255), V255 (= V257), V256 (= V258), K282 (= K284), Y318 (= Y320), T319 (= T321)

6s54A Transaminase from pseudomonas fluorescens (see paper)
51% identity, 96% coverage: 14:445/449 of query aligns to 18:448/453 of 6s54A

query
sites
6s54A

H

H

P

P

M
|
M

S

Q

S

D

S

P

A

K

P

A

A

L

N

H

R

E

S

Q

K

R

T

P

L

L

I

I

I

I

A

Q

R

S

G

G

D

K

G

G

N

V

Y

H

I

I

T

T

D

D

I

V

E

D

G

G

H

R

R

R

M

F

L

I

D

D

G

C

V

Q

G

G

G

G

L

L

W

W

N

C

V

V

N

N

V

A

G

G

H

Y

N

G

R

R

P

R

S

E

V

I

K

I

A

D

A

A

I

V

A

T

A

R

Q

Q

L

M

D

E

E

E

L

L

A

A

Y

Y

Y

Y

Q

S

T

L

F

F

D

P

G

G

I

S

A

T

H

N

P

A

R

P

V

A

F

I

D

A

L

L

A

S

E

Q

R

K

L

L

T

T

S

E

M

V

F

A

A

A

Q

E

E

E

N

G

M

M

A

V

R

K

V

A

L

S

F

F

S

G

S

L

G
|
G

G
|
G

S
|
S

D

D

A

A

V

V

E

E

T

T

A

A

L

L

K

K

M

I

A

A

R

R

Q

Q

Y

Y

W

W

I

K

A

L

S

E

G

G

E

Q

P

P

G

D

R

K

T

V

R

K

F

F

L

V

S

S

L

L

R

Y

N

N

G

G

Y
|
Y

H
|
H

G
|
G

V

L

H

N

V

F

G

G

G

G

T

M

S

S

V

A

G

C

G

G

N

G

G

N

V

A

Y

W

H

K

Y

S

N

S

H

Y

G

E

P

P

L

L

L

M

A

P

G

G

C

F

H

F

L

Q

L

V

D

E

T

S

P

P

W

H

L

L

Y

Y

R

R

N

N

P

P

W

F

D

-

C

T

R

N

D

D

P

P

E

E

E

E

L

L

T

A

A

E

H

I

C

C

I

A

R

Q

Q

I

L

L

E

E

D

R

Q

Q

I

I

A

E

L

M

L

Q

G

A

P

P

Q

G

T

T

I

V

A

A

A

A

L

L

I

I

A

A

E
|
E

P

P

V

I

Q

Q

G

G

A

A

G

G

G

G

V

V

I

I

V

V

P

P

P

P

A

A

H

S

Y

Y

W

W

K

P

R

R

L

L

R

R

E

Q

V

I

C

C

D

D

R

K

H

Y

G

D

I

I

L

L

L

L

I

I

A

A

D
|
D

E

E

V
|
V

V
x
I

T

T

G

G

F

L

G

G

R

R

T

S

G

G

C

S

M

L

L

F

G

G

S

S

R

R

G

G

W

W

G

G

V

V

A

K

P

P

D

D

V

I

L

M

C

C

L

L

A

A

K
|
K

G

G

I

I

T

S

A

S

G

G

Y

Y

I

V

P

P

M

L

G

S

A

A

T

T

V

L

F

V

N

N

Q

S

R

R

I

V

A

A

D

R

A

A

I

W

E

E

N

R

G

D

P

A

G

G

F

F

S

T

S

S

V

V

I

Y

M

M

H

H

G

G

Y
|
Y

T
|
T

Y

Y

S

S

G

G

H

H

P

P

T

V

A

S

C

C

A

A

A

A

A

A

L

L

A

A

V

A

L

I

D

D

I

I

V

V

E

L

A

Q

E

E

D

N

L

L

P

A

G

E

N

N

A

A

G

R

K

V

V

V

G

G

A

D

Q

Y

L

F

L

L

E

E

Q

K

L

L

Q

L

P

I

L

L

T

K

E

D

R

K

Y

H

A

R

V

A

V

I

G

G

E

D

V

V

R

R

G

G

K

K

G

G

L

L

M

M

I

L

A

A

V

V

D

E

L

L

V

V

A

K

D

E

K

R

V

A

T

T

R

K

E

E

P

P

L

F

D

G

P

P

A

A

N

D

G

A

L

Y

A

P

S

L

R

A

I

I

A

S

E

E

Q

A

A

C

R

V

R

N

A

N

G

G

V

V

L

M

V

I

R

R

P

T

I

I

G

V

N

N

K

K

I

L

V

I

M

I

S

S

P

P

P

P

L

L

T

T

L

F

T

T

S

T

D

E

E

H

A

V

A

D

M

E

M

V

V

I

G

E

A

V

L

L

D

D

G

R

A

A

L

F

active site: M20 (= M16), Y152 (= Y148), D258 (= D255), K287 (= K284)
binding pyridoxal-5'-phosphate: G118 (= G114), G119 (= G115), S120 (= S116), Y152 (= Y148), H153 (= H149), G154 (= G150), E225 (= E222), D258 (= D255), V260 (= V257), I261 (≠ V258), K287 (= K284), Y323 (= Y320), T324 (= T321)

6io1B Crystal structure of a novel thermostable (s)-enantioselective omega- transaminase from thermomicrobium roseum (see paper)
44% identity, 97% coverage: 14:448/449 of query aligns to 18:446/448 of 6io1B

query
sites
6io1B

H

H

P

P

M
x
L

S

T

S

N

S

L

A

Y

P

Q

A

L

N

R

R

R

S

E

K

G

T

P

L

L

I

V

I

L

A

V

R

R

G

G

D

E

G

G

N

V

Y

W

I

V

T

W

D

D

I

A

E

E

G

G

H

K

R

R

M

Y

L

L

D

D

G

G

V

F

G

A

G

G

L

L

W

W

N

N

V

V

N

N

V

I

G

G

H

H

N

G

R

R

P

R

S

E

V

L

K

A

A

E

A

A

I

A

A

R

A

E

Q

Q

L

M

D

E

E

R

L

V

A

A

Y

F

Y

V

Q

P

T

T

F

F

D

F

G

G

I

L

A

A

H

S

P

P

R

P

V

T

F

I

D

E

L

L

A

A

E

A

R

R

L

L

T

A

S

E

M

L

F

F

A

P

Q

G

E

-

N

P

M

L

A

D

R

H

V

F

L

Q

F

F

S

T

S

S

G
|
G

G
|
G

S
x
A

D

E

A

S

V
x
N

E

E

T

T

A

A

L

I

K

K

M

I

A

A

R

R

Q

Y

Y

Y

W

W

I

W

A

L

S

K

G

G

E

Q

P

P

G

E

R

R

T

V

R

K

F

I

L

L

S

S

L

R

R

R

N

M

G

A

Y
|
Y

H
|
H

G
|
G

V

I

H

A

V

M

G

G

G

A

T

L

S

S

V

A

G

T

G

G

N

V

G

P

V

A

Y

Y

H

W

Y

E

N

G

H

F

G

G

P

P

L

R

L

P

A

P

G

G

C

F

H

I

L

H

L

L

D

T

T

A

P

P

W

Y

L

K

Y

Y

R

R

N

F

P

G

W

E

D

G

C

L

R

T

D

D

P

-

E

E

E

E

L

F

T

V

A

A

H

R

C

L

I

V

R

Q

Q

E

L

L

E

E

D

E

Q

T

I

I

A

E

L

R

L

E

G

G

P

S

Q

E

T

T

I

I

A

A

A

A

L

F

I

I

A

G

E
|
E

P

P

V

V

Q

Q

G

G

A

A

G

G

G

G

V

V

I

V

V

V

P

P

P

P

A

D

H

G

Y

Y

W

W

K

P

R

A

L

I

R

A

E

A

V

V

C

L

D

R

R

K

H

Y

G

G

I

I

L

L

L

L

I

I

A

L

D
|
D

E

E

V
|
V

V
x
I

T

T

G

G

F

F

G

G

R

R

T

T

G

G

C

T

M

L

L

F

G

G

S

M

R

Q

G

Q

W

Y

G

G

V

V

A

Q

P

P

D

D

V

I

L

V

C

T

L

F

A

A

K
|
K

G

G

I

I

T

T

A

S

G

G

Y

Y

I

V

P

P

M

L

G

G

A

G

T

V

V

G

F

V

N

S

Q

D

R

E

I

I

A

A

D

E

A

T

I

L

E

A

N

S

G

A

P

-

G

-

F

-

S

D

S

R

V

V

I

F

M

M

H

H

G

G

Y

F

T

T

Y

Y

S

S

G

G

H

H

P

P

T

V

A

A

C

C

A

A

A

V

A

A

L

L

A

R

V

N

L

L

D

D

I

I

V

L

E

L

A

A

E

E

D

R

L

L

P

W

G

E

N

N

A

A

G

A

K

A

V

S

G

G

A

A

Q

Y

L

L

L

L

E

Q

Q

E

L

L

Q

K

P

R

L

L

T

E

E

E

R

R

Y

-

A

P

V

Y

V

V

G

G

E

E

V

V

R

R

G

G

K

K

G

G

L

L

M

M

I

L

A

L

V

V

D

E

L

V

V

V

A

R

D

D

K

K

V

A

T

S

R

K

E

E

P

K

L

F

D

P

P

P

A

E

N

F

G

K

L

L

A

G

S

P

R

K

I

L

A

E

E

A

Q

A

A

T

R

R

R

R

A

R

G

G

V

I

L

I

V

V

R

R

P

C

I

T

G

P

N

D

K

G

I

I

V

V

M

M

S

A

P

P

P

P

L

L

T

T

L

I

T

S

S

R

D

A

E

E

A

C

A

D

M

V

M

L

V

I

G

E

A

G

L

V

D

A

G

A

A

A

L

L

A

S

D

D

C

V

active site: L20 (≠ M16), Y151 (= Y148), D257 (= D255), K286 (= K284)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G114), G118 (= G115), A119 (≠ S116), N122 (≠ V119), Y151 (= Y148), H152 (= H149), G153 (= G150), E224 (= E222), D257 (= D255), V259 (= V257), I260 (≠ V258), K286 (= K284)

6fyqA The crystal structure of a new transaminase from the marine bacterium virgibacillus (see paper)
38% identity, 98% coverage: 9:449/449 of query aligns to 8:439/443 of 6fyqA

query
sites
6fyqA

Q

K

K

K

K

H

F

F

W

I

H

H

P

P

M
x
F

S

S

S

S

-

I

S

Q

A

E

P

Q

A

Q

N

H

R

K

S

G

K

A

T

K

L

V

I

I

I

M

A

K

R

E

G

G

D

D

G

G

N

I

Y

Y

I

L

T

T

D

D

I

V

E

T

G

G

H

K

R

T

M

Y

L

I

D

D

G

G

V

V

G

S

G

S

L

L

W

W

N

N

V

V

N

N

V

V

G

G

H

H

N

G

R

R

P

V

S

E

V

L

K

A

A

E

A

A

I

A

A

A

A

Q

Q

Q

L

M

D

K

E

K

L

M

A

A

Y

F

Y

S

Q

S

T

A

F

F

D

S

G

T

I

F

A

S

H

H

P

E

R

P

V

A

F

I

D

R

L

L

A

A

E

E

R

K

L

I

T

A

S

S

M

I

F

-

A

T

Q

P

E

E

N

G

M

L

A

N

R

A

V

V

L

F

F

F

S

T

S

S

G
|
G

G
|
G

S
|
S

D

E

A

S

V
x
N

E

D

T

S

A

A

L

V

K

K

M

L

A

V

R

R

Q

H

Y

Y

W

W

I

K

A

I

S

Q

G

G

E

K

P

P

G

N

R

K

T

R

R

K

F

I

L

I

S

S

L

L

R

K

N

R

G

S

Y
|
Y

H
|
H

G
|
G

V

V

H

A

V

A

G

A

G

S

T

T

S

S

V

V

G

T

G

G

N

I

G

P

V

E

Y

F

H

W

Y

G

N

M

H

A

G

G

P

H

L

M

L

M

A

T

G

D

C

F

H

L

L

H

L

V

D

D

T

T

P

H

W

-

L

-

Y

Y

R

N

N

N

P

T

W

-

D

-

C

-

R

-

D

-

P

-

E

-

E

-

L

-

T

T

A

E

H

Q

C

A

I

V

R

Q

Q

S

L

L

E

C

D

Q

Q

A

I

I

A

E

L

E

L

A

G

G

P

P

Q

E

T

T

I

I

A

A

A

A

L

F

I

F

A

A

E
|
E

P

P

V

V

Q

Q

G

G

A

A

G

G

G

G

V

V

I

I

V

I

P

P

P

P

A

E

H

D

Y

Y

W

F

K

L

R

R

L

I

R

R

E

E

V

V

C

C

D

N

R

A

H

Y

G

G

I

I

L

L

L

F

I

V

A

A

D
|
D

E

E

V
|
V

V
x
I

T

T

G

G

F

F

G

G

R

R

T

T

G

G

C

K

M

M

L

F

G

G

S

I

R

E

G

N

W

W

G

D

V

V

A

I

P

P

D

D

V

V

L

M

C

T

L

F

A

A

K
|
K

G

G

I

V

T

T

A

S

G

G

Y

Y

I

F

P

P

M

L

G

G

A

G

T

V

V

V

F

V

N

S

Q

D

R

P

I

I

A

H

D

E

A

V

I

L

E

K

N

E

G

K

P

S

G

-

F

-

S

V

S

G

V

T

I

L

M

F

H

H

G

G

Y

F

T

T

Y

Y

S

S

G

G

H

H

P

P

T

T

A

A

C

A

A

A

A

V

A

A

L

L

A

K

V

N

L

I

D

A

I

I

V

I

E

K

A

E

E

E

D

R

L

L

P

V

G

E

N

N

A

S

G

K

K

R

V

M

G

G

A

D

Q

A

L

L

L

L

E

H

Q

G

L

L

Q

K

P

K

L

V

T

K

E

N

R

R

Y

L

A

E

V

I

V

V

G

G

E

D

V

V

R

R

G

F

K

V

G

G

L

L

M

L

I

G

A

A

V

V

D

E

L

L

V

M

A

Q

D

N

K

P

V

A

T

T

R

N

E

K

P

P

L

F

D

S

P

S

A

N

N

L

G

Q

L

V

A

A

S

P

R

K

I

V

A

I

E

E

Q

A

A

L

R

H

R

E

A

L

G

G

V

V

L

I

V

C

R

R

P

S

I

V

-

T

-

Y

-

D

-

H

G

T

N

N

K

I

I

I

V

C

M

L

S

A

P

P

P

P

L

L

T

I

L

I

T

N

S

Q

D

K

E

Q

A

V

A

D

M

K

M

L

V

V

G

E

A

V

L

I

D

Y

G

E

A

A

L

I

A

L

D

K

C

V

R

Q

active site: F15 (≠ M16), Y147 (= Y148), D243 (= D255), K272 (= K284)
binding pyridoxal-5'-phosphate: G113 (= G114), G114 (= G115), S115 (= S116), N118 (≠ V119), Y147 (= Y148), H148 (= H149), G149 (= G150), E210 (= E222), D243 (= D255), V245 (= V257), I246 (≠ V258), K272 (= K284)

6gwiB The crystal structure of halomonas elongata amino-transferase (see paper)
38% identity, 95% coverage: 14:438/449 of query aligns to 16:435/450 of 6gwiB

query
sites
6gwiB

H

H

P

P

M
x
F

S

T

S

D

S

F

A

K

P

A

A

L

N

G

R

E

S

E

K

G

T

S

L

R

I

V

I

V

A

T

R

H

G

A

D

E

G

G

N

V

Y

Y

I

I

T

H

D

D

I

S

E

E

G

G

H

N

R

R

M

I

L

L

D

D

G

G

V

M

G

A

G

G

L

L

W

W

N

C

V

V

N

N

V

L

G

G

H

Y

N

G

R

R

P

R

S

E

V

L

K

V

A

E

A

A

I

A

A

T

A

A

Q

Q

L

L

D

E

E

Q

L

L

A

P

Y

Y

Y

Y

Q

N

T

T

F

F

D

F

G

K

I

T

A

T

H

H

P

P

R

P

V

A

F

V

D

R

L

L

A

A

E

E

R

K

L

L

T

C

S

D

M

L

F

-

A

A

Q

P

E

A

N

H

M

I

A

N

R

R

V

V

L

F

F

F

S

T

S

G

G
x
S

G
|
G

S
|
S

D

E

A

A

V
x
N

E

D

T

T

A

V

L

L

K

R

M

M

A

V

R

R

Q

R

Y

Y

W

W

I

A

A

L

S

K

G

G

E

Q

P

P

G

D

R

K

T

Q

R

W

F

I

L

I

S

G

L

R

R

E

N

N

G

A

Y
|
Y

H
|
H

G
|
G

V

S

H

T

V

L

G

A

G

G

T

M

S

S

V

L

G

G

G

G

N

M

G

A

V

P

Y

M

H

H

Y

A

N

Q

H

G

G

G

P

P

L

C

L

V

A

P

G

G

C

I

H

A

L

H

L

I

D

R

T

Q

P

P

W

Y

L

W

Y

F

R

G

N

E

P

G

W

R

D

D

C

M

R

-

D

S

P

P

E

E

E

A

L

F

T

G

A

Q

H

T

C

C

I

A

R

E

Q

A

L

L

E

E

D

E

Q

K

I

I

A

L

L

E

L

L

G

G

P

E

Q

E

T

K

I

V

A

A

A

A

L

F

I

I

A

A

E
|
E

P

P

V

V

Q

Q

G

G

A

A

G

G

G

G

V

A

I

I

V

M

P

P

P

P

A

E

H

S

Y

Y

W

W

K

P

R

A

L

V

R

K

E

K

V

V

C

L

D

A

R

K

H

Y

G

D

I

I

L

L

L

L

I

V

A

A

D
|
D

E

E

V
|
V

V
x
I

T

C

G

G

F

F

G

G

R

R

T

L

G

G

C

E

M

W

L

F

G

G

S

S

R

Q

G

H

W

Y

G

G

V

L

A

E

P

P

D

D

V

L

L

M

C

P

L

I

A

A

K
|
K

G

G

I

L

T

S

A

S

G

G

Y

Y

I

L

P

P

M

I

G

G

A

G

T

V

V

L

F

V

N

G

Q

D

R

R

I

V

A

A

D

E

A

T

-

L

I

I

E

E

N

E

G

G

P

G

G

E

F

F

S

-

S

-

V

-

I

-

M

F

H

H

G

G

Y
x
F

T
|
T

Y

Y

S

S

G

G

H

H

P

P

T

T

A

C

C

A

A

A

A

V

A

A

L

L

A

K

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N

L

L

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L

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L

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L

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D

E

E

E

A

I

A

D

M

E

M

L

V

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active site: F18 (≠ M16), Y149 (= Y148), D255 (= D255), K284 (= K284)
binding pyridoxal-5'-phosphate: S115 (≠ G114), G116 (= G115), S117 (= S116), N120 (≠ V119), Y149 (= Y148), H150 (= H149), G151 (= G150), E222 (= E222), D255 (= D255), V257 (= V257), I258 (≠ V258), K284 (= K284), F317 (≠ Y320), T318 (= T321)

Q94CE5 Gamma-aminobutyrate transaminase POP2, mitochondrial; AtGABA-T; Gamma-aminobutyric acid aminotransferase 1; Protein HEXENAL RESPONSE 1; Protein POLLEN-PISTIL INCOMPATIBILITY 2; AtPOP2; EC 2.6.1.96 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
36% identity, 93% coverage: 28:445/449 of query aligns to 71:488/504 of Q94CE5

query
sites
Q94CE5

L

L

I

V

I

I

A

A

R

K

G

S

D

E

G

G

N

S

Y

Y

I

V

T

Y

D

D

I

D

E

T

G

G

H

K

R

K

M

Y

L

L

D

D

G

S

V

L

G

A

G

G

L

L

W

W

N
x
C

V

T

N

A

V

L

G

G

H
x
G

N

N

R

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P

P

S

R

V

L

K

V

A

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A

A

I

A

A

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Q

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L

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N

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L

L

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M

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N

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M

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F

F

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I

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G

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M

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S

A

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L

C98 (≠ N55) mutation to Y: In her1-1; loss of E2-hexenal responses.
G103 (≠ H60) mutation to N: In her1-2; loss of E2-hexenal responses.
G271 (= G228) mutation to E: In pop2-6; Loss of activity and complete sterility.
G312 (= G269) mutation to D: In pop2-7; loss of activity.

5kquC Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
37% identity, 98% coverage: 5:445/449 of query aligns to 8:451/459 of 5kquC

query
sites
5kquC

Y

F

K

E

T

Q

A

D

Q

R

K

A

K

H

F

F

W

M

H

H

P

P

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L

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L

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x
S

G
|
G

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|
S

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D

A

A

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N

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K

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G

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L

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R

G

G

Y
|
Y

H
|
H

G
|
G

V

S

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G

V

I

G

M

G

T

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|
E

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V

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F

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L

L

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L

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|
K

G

G

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Y

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M

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x
W

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|
T

Y

Y

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G

H

H

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P

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C

A

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L

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M

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-

A

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M

P

P

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H

G

G

N

D

K

I

I

L

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L

L

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L

L

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A

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A

D

M

E

M

I

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V

G

G

A

I

L

A

D

K

G

A

A

A

L

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binding pyridoxal-5'-phosphate: S119 (≠ G114), G120 (= G115), S121 (= S116), Y153 (= Y148), H154 (= H149), G155 (= G150), E226 (= E222), D259 (= D255), V261 (= V257), K288 (= K284), W324 (≠ Y320), T325 (= T321)

5kr6B Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
38% identity, 95% coverage: 12:438/449 of query aligns to 17:446/460 of 5kr6B

query
sites
5kr6B

F

F

W

F

H

H

P

P

M

-

S

S

S

T

S

H

A

L

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S

M

G
x
S

G
|
G

S
|
S

D

D

A

A

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x
N

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T

T

A

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L

I

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K

M

L

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|
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F

F

G

G

R

R

T

T

G

G

C

S

M

M

L

F

G

G

S

S

R

H

G

H

W

Y

G

G

V

M

A

K

P

P

D

D

V

L

L

I

C

T

L

V

A

A

K
|
K

G

G

I

L

T

T

A

S

G

A

Y

Y

I

A

P

P

M

L

G

S

A

G

T

V

V

I

F

V

N

G

Q

E

R

K

I

V

A

W

D

K

A

V

I

L

E

E

N

Q

G

G

P

S

G

D

F

Q

S

Y

S

G

V

P

I

I

M

G

H

H

G

G

Y
x
W

T
|
T

Y

Y

S

S

G

G

H

H

P

P

T

I

A

C

C

A

A

A

A

A

A

A

L

L

A

A

V

N

L

L

D

D

I

I

V

I

E

E

A

R

E

E

D

N

L

L

P

T

G

G

N

N

A

A

G

A

K

D

V

T

G

G

A

A

Q

Y

L

F

L

Q

E

Q

Q

R

L

M

Q

R

P

E

L

T

T

F

E

G

R

D

Y

H

A

P

V

L

V

V

G

G

E

E

V

V

R

R

G

G

K

V

G

G

L

L

M

M

I

A

A

A

V

L

D

E

L

F

V

V

A

A

D

D

K

K

V

D

T

K

R

R

E

T

P

R

L

F

D

D

P

P

A

S

N

L

G

K

L

V

A

G

S

P

R

R

I

V

A

S

E

A

Q

A

A

C

R

L

R

E

A

D

G

G

V

M

L

I

V

A

R

R

-

A

-

M

P

P

I

H

G

G

N

D

K

I

I

L

V

G

M

F

S

A

P

P

P

P

L

L

T

V

L

I

T

T

S

R

D

A

E

E

A

V

A

D

M

E

M

I

V

V

binding pyridoxal-5'-phosphate: S121 (≠ G114), G122 (= G115), S123 (= S116), N126 (≠ V119), Y155 (= Y148), H156 (= H149), G157 (= G150), E228 (= E222), D261 (= D255), V263 (= V257), V264 (= V258), K290 (= K284), W326 (≠ Y320), T327 (= T321)

5kr3A Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
38% identity, 97% coverage: 5:439/449 of query aligns to 9:444/458 of 5kr3A

query
sites
5kr3A

Y

F

K

E

T

M

A

D

Q

R

K

A

K

H

F

F

W

M

H

H

P

P

M

-

S

S

S

T

S

H

A

A

P

H

A

D

N

H

R

A

S

S

K

G

T

A

L

L

-

P

-

G

-

R

I

I

I

I

A

T

R

G

G

G

D

K

G

G

N

I

Y

R

I

I

T

E

D

D

I

H

E

E

G

G

H

R

R

E

M

Y

L

I

D

D

G

A

V

F

G

A

G

G

L

L

W

Y

N

C

V

V

N

N

V

I

G

G

H

Y

N

G

R

R

P

T

S

E

V

V

K

A

A

D

A

A

I

I

A

Y

A

E

Q

Q

L

A

D

K

E

Q

L

L

A

A

Y

Y

Y

Y

Q

H

T

T

F

Y

D

V

G

G

I

H

A

S

H

T

P

D

R

A

V

I

F

I

D

E

L

L

A

S

E

S

R

R

L

I

T

I

S

R

M

D

F

W

A

A

Q

P

E

A

N

G

M

M

A

K

R

K

V

V

L

Y

F

Y

S

G

S

M

G
x
S

G
|
G

S
|
S

D

D

A

A

V
x
N

E

E

T

T

A

Q

L

I

K

K

M

L

A

V

R

W

Q

Y

Y

Y

W

N

I

N

A

V

S

L

G

G

E

R

P

P

G

N

R

K

T

K

R

K

F

I

L

I

S

S

L

R

R

E

N

R

G

G

Y
|
Y

H
|
H

G
|
G

V

S

H

G

V

I

G

V

G

T

T

G

S

S

V

L

G

T

G

G

N

L

G

P

V

S

Y

F

H

H

Y

Q

N

H

H

F

G

D

P

L

L

P

L

I

A

D

G

R

C

V

H

K

L

H

L

T

D

V

T

C

P

P

W

H

L

W

Y

Y

R

R

N

A

P

P

W

-

D

-

C

-

R

M

D

S

P

E

E

A

E

Q

L

F

T

V

A

A

H

Y

C

C

I

V

R

E

Q

E

L

L

E

E

D

K

Q

L

I

I

A

A

L

R

L

E

G

G

P

A

Q

D

T

T

I

I

A

A

A

A

L

F

I

I

A

A

E
|
E

P

P

V

V

Q

M

G

G

A

T

G

G

G

G

V

I

I

I

V

P

P

P

P

P

A

Q

H

G

Y

Y

W

W

K

E

R

A

L

I

R

Q

E

A

V

V

C

L

D

R

R

K

H

H

G

D

I

I

L

L

L

L

I

I

A

A

D
|
D

E

E

V
|
V

V

V

T

C

G

G

F

F

G

G

R

R

T

L

G

G

C

S

M

K

L

M

G

G

S

S

R

Q

G

H

W

Y

G

G

V

I

A

K

P

P

D

D

V

L

L

I

C

T

L

V

A

A

K
|
K

G

G

I

L

T

T

A

S

G

A

Y

Y

I

A

P

P

M

L

G

S

A

G

T

V

V

I

F

V

N

G

Q

E

R

K

I

V

A

W

D

D

A

V

I

I

E

E

N

K

G

G

P

S

G

Q

F

E

S

H

S

G

V

P

I

M

M

G

H

H

G

G

Y
x
W

T
|
T

Y

Y

S

S

G

G

H

H

P

P

T

I

A

C

C

A

A

A

A

A

A

A

L

L

A

A

V

N

L

L

D

D

I

I

V

L

E

E

A

R

E

E

D

N

L

L

P

T

G

G

N

N

A

A

G

A

K

D

V

V

G

G

A

A

Q

Y

L

L

L

Q

E

Q

Q

R

L

L

Q

H

P

E

L

A

T

F

E

G

R

A

Y

H

A

P

V

L

V

V

G

G

E

E

V

V

R

R

G

G

K

V

G

G

L

M

M

L

I

A

A

A

V

L

D

E

L

F

V

M

A

A

D

D

K

K

V

D

T

A

R

R

E

T

P

P

L

F

D

D

P

P

A

A

N

L

G

K

L

V

A

G

S

P

R

K

I

V

A

S

E

A

Q

A

A

A

R

L

R

E

A

D

G

G

V

M

L

I

V

A

R

R

-

A

-

M

P

P

I

H

G

G

N

D

K

I

I

L

V

G

M

F

S

A

P

P

P

P

L

L

T

V

L

T

T

T

S

R

D

A

E

E

A

V

A

D

M

E

M

I

V

V

G

G

binding pyridoxal-5'-phosphate: S120 (≠ G114), G121 (= G115), S122 (= S116), N125 (≠ V119), Y154 (= Y148), H155 (= H149), G156 (= G150), E225 (= E222), D258 (= D255), V260 (= V257), K287 (= K284), W323 (≠ Y320), T324 (= T321)

5kr5A Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
39% identity, 95% coverage: 12:438/449 of query aligns to 13:442/455 of 5kr5A

query
sites
5kr5A

F

F

W

M

H

H

P

P

M

-

S

S

S

T

S

H

A

A

P

Y

A

D

N

H

R

A

S

S

K

G

T

A

L

L

-

P

-

G

-

R

I

I

I

I

A

T

R

G

G

G

D

K

G

G

N

I

Y

R

I

I

T

E

D

D

I

H

E

E

G

G

H

R

R

E

M

Y

L

I

D

D

G

A

V

F

G

A

G

G

L

L

W

Y

N

C

V

V

N

N

V

I

G

G

H

Y

N

G

R

R

P
x
E

S

E

V

V

K

A

A
x
D

A

A

I

I

A

Y

A

E

Q

Q

L

A

D

K

E

Q

L

L

A

A

Y

Y

Y

Y

Q

H

T

T

F

Y

D

V

G

G

I

H

A

S

H

N

P

D

R

P

V

V

F

I

D

E

L

L

A

S

E

S

R

R

L

I

T

I

S

E

M

D

F

W

A

A

Q

P

E

A

N

G

M

M

A

K

R

K

V

V

L

F

F

Y

S

G

S

M

G
x
S

G
|
G

S
|
S

D

D

A

A

V
x
N

E

E

T

T

A

Q

L

I

K

K

M

L

A

V

R

W

Q

Y

Y

Y

W

N

I

N

A

V

S

L

G

G

E

R

P

P

G

N

R

K

T

K

R

K

F

I

L

I

S