SitesBLAST
Comparing AO356_28740 FitnessBrowser__pseudo5_N2C3_1:AO356_28740 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8y46A SDR family oxidoreductase (see paper)
62% identity, 99% coverage: 3:245/246 of query aligns to 5:250/251 of 8y46A
8y46B SDR family oxidoreductase (see paper)
62% identity, 99% coverage: 3:245/246 of query aligns to 2:247/248 of 8y46B
8y4jA SDR family oxidoreductase (see paper)
62% identity, 99% coverage: 3:245/246 of query aligns to 3:248/249 of 8y4jA
8y11A Crystal structure of l-2-keto-3-deoxyfuconate 4-dehydrogenase bound to NAD(h) and sulfate ion (see paper)
62% identity, 99% coverage: 3:245/246 of query aligns to 3:248/249 of 8y11A
- binding nicotinamide-adenine-dinucleotide: A12 (= A12), Q15 (= Q15), G16 (= G16), I17 (= I17), D36 (= D36), I37 (= I37), D57 (= D54), V58 (= V55), C80 (= C77), I130 (≠ M127), Y146 (= Y143), K150 (= K147), P176 (= P173), G177 (= G174), T178 (= T175), I179 (≠ V176), S181 (= S178), P182 (= P179), S183 (= S180), R187 (= R184)
D4A1J4 Dehydrogenase/reductase SDR family member 6; (R)-beta-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase type 2; 4-oxo-L-proline reductase; Oxidoreductase UCPA; Short chain dehydrogenase/reductase family 15C member 1; EC 1.1.1.-; EC 1.1.1.30; EC 1.1.1.104 from Rattus norvegicus (Rat) (see paper)
56% identity, 99% coverage: 3:246/246 of query aligns to 4:245/245 of D4A1J4
- Y147 (= Y143) mutation to F: Loss of function.
Q8JZV9 Dehydrogenase/reductase SDR family member 6; (R)-beta-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase type 2; 4-oxo-L-proline reductase; Oxidoreductase UCPA; Short chain dehydrogenase/reductase family 15C member 1; EC 1.1.1.-; EC 1.1.1.30; EC 1.1.1.104 from Mus musculus (Mouse) (see paper)
55% identity, 99% coverage: 3:246/246 of query aligns to 4:245/245 of Q8JZV9
- Y147 (= Y143) active site, Proton acceptor; mutation to F: Loss of function.
Q9BUT1 Dehydrogenase/reductase SDR family member 6; (R)-beta-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase type 2; 4-oxo-L-proline reductase; Oxidoreductase UCPA; Short chain dehydrogenase/reductase family 15C member 1; EC 1.1.1.-; EC 1.1.1.30; EC 1.1.1.104 from Homo sapiens (Human) (see 4 papers)
55% identity, 99% coverage: 3:246/246 of query aligns to 4:245/245 of Q9BUT1
- QGI 16:18 (= QGI 15:17) binding NAD(+)
- D37 (= D36) binding NAD(+)
- D58 (= D54) binding NAD(+)
- N70 (≠ E66) to S: in dbSNP:rs1054707
- K151 (= K147) binding NAD(+)
- VDTPS 180:184 (≠ VDSPS 176:180) binding NAD(+)
2ag5A Crystal structure of human dhrs6 (see paper)
55% identity, 99% coverage: 3:246/246 of query aligns to 4:245/246 of 2ag5A
- active site: S133 (= S129), Y147 (= Y143), K151 (= K147), R192 (≠ G193)
- binding nicotinamide-adenine-dinucleotide: Q16 (= Q15), G17 (= G16), I18 (= I17), D37 (= D36), I38 (= I37), D58 (= D54), V59 (= V55), V81 (≠ C77), G83 (= G79), L104 (= L100), Y147 (= Y143), K151 (= K147), P177 (= P173), V180 (= V176), T182 (≠ S178), S184 (= S180)
- binding sulfate ion: R144 (= R140), R188 (= R184), F202 (= F203), R205 (= R206)
4gh5A Crystal structure of s-2-hydroxypropyl coenzyme m dehydrogenase (s- hpcdh) (see paper)
39% identity, 89% coverage: 27:245/246 of query aligns to 27:246/248 of 4gh5A
- active site: N113 (= N101), S141 (= S129), Y154 (= Y143), K158 (= K147)
- binding nicotinamide-adenine-dinucleotide: D36 (= D36), L37 (≠ I37), A61 (vs. gap), D62 (= D54), V63 (= V55), N89 (≠ C77), A90 (= A78), V112 (≠ L100), F139 (≠ M127), S141 (= S129), Y154 (= Y143), K158 (= K147), P184 (= P173), V187 (= V176), T190 (≠ P179), G191 (≠ S180), M192 (≠ L181)
Sites not aligning to the query:
4ituA Crystal structure of s-2-hydroxypropyl coenzyme m dehydrogenase (s- hpcdh) bound to s-hpc and nadh (see paper)
39% identity, 89% coverage: 27:245/246 of query aligns to 27:251/253 of 4ituA
- active site: N113 (= N101), S141 (= S129), Y154 (= Y143), K158 (= K147)
- binding 2-{[(2S)-2-hydroxypropyl]sulfanyl}ethanesulfonic acid: S141 (= S129), Y154 (= Y143), T186 (= T175), R209 (= R201), Y213 (≠ Q207)
- binding 1,4-dihydronicotinamide adenine dinucleotide: D36 (= D36), L37 (≠ I37), D62 (= D54), V63 (= V55), N89 (≠ C77), V112 (≠ L100), F139 (≠ M127), S141 (= S129), Y154 (= Y143), K158 (= K147), P184 (= P173), T186 (= T175), V187 (= V176), T190 (≠ P179), M192 (≠ L181)
Sites not aligning to the query:
A7IQH5 2-(S)-hydroxypropyl-CoM dehydrogenase 3; S-HPCDH 3; 2-[(S)-2-hydroxypropylthio]ethanesulfonate dehydrogenase 3; Aliphatic epoxide carboxylation component IV; Epoxide carboxylase component IV; SHPCDH3; EC 1.1.1.269 from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) (see 2 papers)
39% identity, 89% coverage: 27:245/246 of query aligns to 29:253/255 of A7IQH5
- D38 (= D36) binding NAD(+)
- DV 64:65 (= DV 54:55) binding NAD(+)
- N91 (≠ C77) binding NAD(+)
- S143 (= S129) binding (S)-2-hydroxypropyl-coenzyme M; mutation to A: Retains very weak activity.
- Y156 (= Y143) binding (S)-2-hydroxypropyl-coenzyme M; mutation to A: Retains some activity but with more than 2200-fold decrease in catalytic efficiency.; mutation to F: Loss of activity.
- K160 (= K147) binding NAD(+); mutation to A: Loss of activity.
- T188 (= T175) binding (S)-2-hydroxypropyl-coenzyme M
- VTSTG 189:193 (≠ VDSPS 176:180) binding NAD(+)
- R211 (= R201) mutation to A: Severely impaired in the oxidation of S-HPC or reduction of 2-KPC but largely unaffected in the oxidation and reduction of aliphatic alcohols and ketones.
- K214 (≠ R206) mutation to A: Severely impaired in the oxidation of S-HPC or reduction of 2-KPC but largely unaffected in the oxidation and reduction of aliphatic alcohols and ketones.
- Y215 (≠ Q207) binding (S)-2-hydroxypropyl-coenzyme M
Sites not aligning to the query:
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
35% identity, 100% coverage: 1:245/246 of query aligns to 3:253/255 of 5itvA
- active site: G18 (= G16), S141 (= S129), Y154 (= Y143), K158 (= K147)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (≠ A12), S17 (≠ Q15), G18 (= G16), I19 (= I17), D38 (= D36), I39 (= I37), T61 (vs. gap), I63 (≠ V55), N89 (≠ C77), G91 (= G79), T139 (≠ M127), S141 (= S129), Y154 (= Y143), K158 (= K147), P184 (= P173), G185 (= G174), I186 (≠ T175), I187 (≠ V176)
2dtxA Structure of thermoplasma acidophilum aldohexose dehydrogenase (aldt) in complex with d-mannose (see paper)
35% identity, 100% coverage: 2:246/246 of query aligns to 4:247/255 of 2dtxA
2dteA Structure of thermoplasma acidophilum aldohexose dehydrogenase (aldt) in complex with nadh (see paper)
35% identity, 100% coverage: 2:246/246 of query aligns to 4:247/255 of 2dteA
- active site: G18 (= G16), S132 (= S129), Y145 (= Y143), S148 (= S146), K149 (= K147)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (≠ A12), S16 (≠ G14), M17 (≠ Q15), G18 (= G16), I19 (= I17), S38 (≠ D38), I39 (= I39), C52 (≠ L53), D53 (= D54), V54 (= V55), N80 (≠ C77), A81 (= A78), I130 (≠ M127), S132 (= S129), Y145 (= Y143), K149 (= K147), P174 (= P173), A175 (≠ G174), T176 (= T175), I177 (≠ V176), T179 (≠ S178), P180 (= P179), L181 (≠ S180), V182 (≠ L181)
8y83A Crystal structure of a ketoreductase from sphingobacterium siyangense sy1 with co-enzyme (see paper)
33% identity, 99% coverage: 3:245/246 of query aligns to 4:247/249 of 8y83A
- binding nicotinamide-adenine-dinucleotide: G13 (≠ A12), S16 (≠ Q15), G17 (= G16), I18 (= I17), D37 (= D36), I38 (= I37), A62 (≠ L53), D63 (= D54), S64 (≠ V55), N90 (≠ C77), M141 (= M127), Y156 (= Y143), K160 (= K147), P186 (= P173), G187 (= G174), Y188 (≠ T175), I189 (≠ V176), L193 (≠ S180)
5b4tA Crystal structure of d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis complexed with NAD+ and a substrate d-3- hydroxybutyrate (see paper)
35% identity, 99% coverage: 3:245/246 of query aligns to 2:258/260 of 5b4tA
- active site: G15 (= G16), N114 (= N101), S142 (= S129), Y155 (= Y143), K159 (= K147), I200 (≠ Q188)
- binding (3R)-3-hydroxybutanoic acid: Q94 (≠ V81), S142 (= S129), H144 (≠ A131), K152 (≠ R140), Y155 (= Y143), W187 (≠ T175), Q196 (≠ R184)
- binding nicotinamide-adenine-dinucleotide: G11 (≠ A12), T13 (≠ G14), G15 (= G16), I16 (= I17), F36 (vs. gap), D63 (= D54), L64 (≠ V55), N90 (≠ C77), G92 (= G79), L113 (= L100), I140 (≠ M127), Y155 (= Y143), K159 (= K147), P185 (= P173), G186 (= G174), W187 (≠ T175), V188 (= V176), T190 (≠ S178), L192 (≠ S180), V193 (≠ L181)
3w8dA Crystal structure of d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis complexed with NAD+ and an inhibitor methylmalonate
35% identity, 99% coverage: 3:245/246 of query aligns to 2:258/260 of 3w8dA
- active site: G15 (= G16), N114 (= N101), S142 (= S129), Y155 (= Y143), K159 (= K147), I200 (≠ Q188)
- binding methylmalonic acid: Q94 (≠ V81), S142 (= S129), H144 (≠ A131), K152 (≠ R140), Y155 (= Y143), W187 (≠ T175), Q196 (≠ R184), W257 (≠ M244)
- binding nicotinamide-adenine-dinucleotide: G11 (≠ A12), T13 (≠ G14), S14 (≠ Q15), G15 (= G16), I16 (= I17), F36 (vs. gap), A62 (≠ L53), D63 (= D54), L64 (≠ V55), N90 (≠ C77), A91 (= A78), G92 (= G79), L113 (= L100), S142 (= S129), Y155 (= Y143), K159 (= K147), P185 (= P173), G186 (= G174), W187 (≠ T175), V188 (= V176), T190 (≠ S178), L192 (≠ S180), V193 (≠ L181)
3vdrA Crystal structure of d-3-hydroxybutyrate dehydrogenase, prepared in the presence of the substrate d-3-hydroxybutyrate and NAD(+) (see paper)
35% identity, 99% coverage: 3:245/246 of query aligns to 2:258/260 of 3vdrA
- active site: G15 (= G16), N114 (= N101), S142 (= S129), Y155 (= Y143), K159 (= K147), I200 (≠ Q188)
- binding (3R)-3-hydroxybutanoic acid: Q94 (≠ V81), H144 (≠ A131), K152 (≠ R140), Y155 (= Y143), W187 (≠ T175), Q196 (≠ R184), W257 (≠ M244)
- binding acetoacetic acid: Q94 (≠ V81), H144 (≠ A131), K152 (≠ R140), Y155 (= Y143), W187 (≠ T175), Q196 (≠ R184), W257 (≠ M244)
- binding nicotinamide-adenine-dinucleotide: G11 (≠ A12), T13 (≠ G14), I16 (= I17), F36 (vs. gap), D63 (= D54), L64 (≠ V55), N90 (≠ C77), A91 (= A78), G92 (= G79), L113 (= L100), K159 (= K147), G186 (= G174), V188 (= V176), T190 (≠ S178), L192 (≠ S180), V193 (≠ L181)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (≠ A12), T13 (≠ G14), I16 (= I17), F36 (vs. gap), D63 (= D54), L64 (≠ V55), N90 (≠ C77), A91 (= A78), G92 (= G79), L113 (= L100), S142 (= S129), Y155 (= Y143), K159 (= K147), G186 (= G174), V188 (= V176), T190 (≠ S178), L192 (≠ S180), V193 (≠ L181)
3vdqA Crystal structure of alcaligenes faecalis d-3-hydroxybutyrate dehydrogenase in complex with NAD(+) and acetate (see paper)
35% identity, 99% coverage: 3:245/246 of query aligns to 2:258/260 of 3vdqA
- active site: G15 (= G16), N114 (= N101), S142 (= S129), Y155 (= Y143), K159 (= K147), I200 (≠ Q188)
- binding acetate ion: Q94 (≠ V81), H144 (≠ A131), K152 (≠ R140), W187 (≠ T175), L192 (≠ S180), Q196 (≠ R184)
- binding nicotinamide-adenine-dinucleotide: G11 (≠ A12), S14 (≠ Q15), I16 (= I17), F36 (vs. gap), D63 (= D54), L64 (≠ V55), N90 (≠ C77), A91 (= A78), G92 (= G79), L113 (= L100), I140 (≠ M127), S142 (= S129), Y155 (= Y143), K159 (= K147), P185 (= P173), G186 (= G174), W187 (≠ T175), V188 (= V176), T190 (≠ S178), L192 (≠ S180), V193 (≠ L181)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
33% identity, 99% coverage: 3:245/246 of query aligns to 3:246/248 of 6ixmC
- active site: G16 (= G16), S142 (= S129), Y155 (= Y143), K159 (= K147)
- binding nicotinamide-adenine-dinucleotide: G12 (≠ A12), S15 (≠ Q15), G16 (= G16), I17 (= I17), D36 (= D36), I37 (= I37), A61 (≠ L53), D62 (= D54), T63 (≠ V55), N89 (≠ C77), A90 (= A78), M140 (= M127), S142 (= S129), Y155 (= Y143), K159 (= K147), P185 (= P173), A186 (≠ G174), Y187 (≠ T175), I188 (≠ V176), L192 (≠ S180)
Query Sequence
>AO356_28740 FitnessBrowser__pseudo5_N2C3_1:AO356_28740
MNLQNKRVLVTAAGQGIGLASAIAFARAGAEVIATDIDIHALAGIEEVTAMSLDVTSTAA
ISAACERIGGLDVLFNCAGYVHSGNILQCDEAAWARSMDLNVTAMYRMIHAFLPGMLARG
GGSIINMSSVASSVKGVPNRFAYATSKAAVVGLTKAVAIDFVSQGIRCNAICPGTVDSPS
LRQRIADQAVQQGVDEAQVYRQFLDRQPMGRIGSTEEIAQLAVYLGSDASAYTTGAIHVI
DGGMSI
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory