SitesBLAST – Find functional sites

 

SitesBLAST

Comparing AO356_28755 FitnessBrowser__pseudo5_N2C3_1:AO356_28755 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

2q1dX 2-keto-3-deoxy-d-arabinonate dehydratase complexed with magnesium and 2,5-dioxopentanoate (see paper)
33% identity, 39% coverage: 182:336/393 of query aligns to 104:254/281 of 2q1dX

query
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2q1dX
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Sites not aligning to the query:

3bqbX Hexagonal kristal form of 2-keto-3-deoxyarabinonate dehydratase (see paper)
33% identity, 39% coverage: 182:336/393 of query aligns to 114:264/291 of 3bqbX

query
sites
3bqbX
E
 
E
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I
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K
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2q1cX 2-keto-3-deoxy-d-arabinonate dehydratase complexed with calcium and 2- oxobutyrate (see paper)
33% identity, 39% coverage: 182:336/393 of query aligns to 114:264/291 of 2q1cX

query
sites
2q1cX
E
 
E
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I
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F
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F
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K
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Sites not aligning to the query:

2q1aX 2-keto-3-deoxy-d-arabinonate dehydratase complexed with magnesium and 2-oxobutyrate (see paper)
33% identity, 39% coverage: 182:336/393 of query aligns to 114:264/291 of 2q1aX

query
sites
2q1aX
E
 
E
I
 
I
F
 
F
T
 
F
K
 
K
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A
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T
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P
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N
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E
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A
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Q
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Sites not aligning to the query:

Q97UA0 2-dehydro-3-deoxy-D-arabinonate dehydratase; 2-keto-3-deoxy-D-arabinonate dehydratase; KdaD; EC 4.2.1.141 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
33% identity, 39% coverage: 182:336/393 of query aligns to 119:269/298 of Q97UA0

query
sites
Q97UA0
E
 
E
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I
F
 
F
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F
K
 
K
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A
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T
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P
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V
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G
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G
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E
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A
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A
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W
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P
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A
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G
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K
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I
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Y
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M
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R
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D
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L
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E
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E
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P
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L
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Y
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P
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Q
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I
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Y
N
 
A
A
 
G
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C
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C
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A
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D
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K

Sites not aligning to the query:

Query Sequence

>AO356_28755 FitnessBrowser__pseudo5_N2C3_1:AO356_28755
MSLMLTPENTLPVDGVAGTLIGRAWVPGRVAGPSPVVLRSDGVFDLSDRFATLSDLLETS
SPLAEVRQTPGIFIASVEALLANTGIHADPAKPFLLPPLDLQVIKAAGVTFAASMIERVI
EEQAGGDAARAEAVRATVHSVIGDNLRTIVPGSEQAMRLKALLIEQGMWSQYLEVGIGPD
AEIFTKAPILSAVGSGSQIGIHPVSHWNNPEPEVVLAVNSRGQIHGATLGNDVNLRDIEG
RSALLLSKAKDNNASCSIGPFIRLFDEHFSLDDVRACVVDLQVQGEDGYRLHGSSSMSQI
SRDPQDLASQTLNANHQYPDGFVLFLGTLFAPTEDRDHPGSGFTHKRGDRVSISSPLLGS
LHNEVTHSCDAAPWTFGVSALLRNLAARGLLGR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory