SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO356_28755 FitnessBrowser__pseudo5_N2C3_1:AO356_28755 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

2q1dX 2-keto-3-deoxy-d-arabinonate dehydratase complexed with magnesium and 2,5-dioxopentanoate (see paper)
33% identity, 39% coverage: 182:336/393 of query aligns to 104:254/281 of 2q1dX

query
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2q1dX

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binding 2,5-dioxopentanoic acid: E104 (= E182), E133 (= E211), E135 (= E213), E161 (= E239), L168 (= L246), G245 (= G327), T246 (= T328)
binding magnesium ion: E133 (= E211), E135 (= E213), D154 (= D232)

Sites not aligning to the query:

binding 2,5-dioxopentanoic acid: 80, 81

3bqbX Hexagonal kristal form of 2-keto-3-deoxyarabinonate dehydratase (see paper)
33% identity, 39% coverage: 182:336/393 of query aligns to 114:264/291 of 3bqbX

query
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3bqbX

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binding magnesium ion: E145 (= E213), D164 (= D232)

2q1cX 2-keto-3-deoxy-d-arabinonate dehydratase complexed with calcium and 2- oxobutyrate (see paper)
33% identity, 39% coverage: 182:336/393 of query aligns to 114:264/291 of 2q1cX

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2q1cX

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binding 2-ketobutyric acid: E143 (= E211), T256 (= T328)
binding calcium ion: E143 (= E211), E145 (= E213), D164 (= D232)

Sites not aligning to the query:

binding 2-ketobutyric acid: 80, 81, 89

2q1aX 2-keto-3-deoxy-d-arabinonate dehydratase complexed with magnesium and 2-oxobutyrate (see paper)
33% identity, 39% coverage: 182:336/393 of query aligns to 114:264/291 of 2q1aX

query
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2q1aX

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binding 2-ketobutyric acid: E143 (= E211), G255 (= G327), T256 (= T328)
binding magnesium ion: E143 (= E211), E145 (= E213), D164 (= D232)

Sites not aligning to the query:

binding 2-ketobutyric acid: 80, 81, 89

Q97UA0 2-dehydro-3-deoxy-D-arabinonate dehydratase; 2-keto-3-deoxy-D-arabinonate dehydratase; KdaD; EC 4.2.1.141 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
33% identity, 39% coverage: 182:336/393 of query aligns to 119:269/298 of Q97UA0

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Q97UA0

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E148 (= E211) binding Mg(2+)
E150 (= E213) binding Mg(2+)
D169 (= D232) binding Mg(2+)
K187 (= K250) binding substrate
T261 (= T328) binding substrate

Sites not aligning to the query:

86 binding substrate

Query Sequence

>AO356_28755 FitnessBrowser__pseudo5_N2C3_1:AO356_28755
MSLMLTPENTLPVDGVAGTLIGRAWVPGRVAGPSPVVLRSDGVFDLSDRFATLSDLLETS
SPLAEVRQTPGIFIASVEALLANTGIHADPAKPFLLPPLDLQVIKAAGVTFAASMIERVI
EEQAGGDAARAEAVRATVHSVIGDNLRTIVPGSEQAMRLKALLIEQGMWSQYLEVGIGPD
AEIFTKAPILSAVGSGSQIGIHPVSHWNNPEPEVVLAVNSRGQIHGATLGNDVNLRDIEG
RSALLLSKAKDNNASCSIGPFIRLFDEHFSLDDVRACVVDLQVQGEDGYRLHGSSSMSQI
SRDPQDLASQTLNANHQYPDGFVLFLGTLFAPTEDRDHPGSGFTHKRGDRVSISSPLLGS
LHNEVTHSCDAAPWTFGVSALLRNLAARGLLGR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory