SitesBLAST
Comparing AO356_28790 FitnessBrowser__pseudo5_N2C3_1:AO356_28790 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7qgtB Crystal structure of human cystathionine beta-synthase (delta516-525) in complex with aoaa. (see paper)
33% identity, 94% coverage: 2:328/347 of query aligns to 36:368/500 of 7qgtB
- binding protoporphyrin ix containing fe: R184 (≠ D148), A186 (= A150), P189 (≠ A153), L190 (vs. gap), Y193 (= Y156), R226 (= R189), T273 (≠ L233), V274 (= V234)
- binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: K79 (= K41), T106 (≠ S68), S107 (= S69), N109 (= N71), T110 (= T72), Q182 (= Q146), S214 (≠ C177), V215 (= V178), G216 (= G179), T217 (= T180), G218 (= G181), G219 (= G182), T220 (≠ S183), E264 (≠ A227), G265 (= G228), I266 (vs. gap), S309 (= S269), P335 (≠ A295), D336 (= D296)
Sites not aligning to the query:
7qgtA Crystal structure of human cystathionine beta-synthase (delta516-525) in complex with aoaa. (see paper)
33% identity, 94% coverage: 2:328/347 of query aligns to 36:368/500 of 7qgtA
- binding protoporphyrin ix containing fe: R184 (≠ D148), A186 (= A150), P189 (≠ A153), L190 (vs. gap), Y193 (= Y156), T222 (= T185), R226 (= R189), T273 (≠ L233), V274 (= V234)
- binding pyridoxal-5'-phosphate: K79 (= K41), N109 (= N71), S214 (≠ C177), V215 (= V178), G216 (= G179), T217 (= T180), G218 (= G181), G219 (= G182), T220 (≠ S183), E264 (≠ A227), G265 (= G228), I266 (vs. gap), S309 (= S269), P335 (≠ A295), D336 (= D296)
Sites not aligning to the query:
P35520 Cystathionine beta-synthase; Beta-thionase; Serine sulfhydrase; EC 4.2.1.22 from Homo sapiens (Human) (see 40 papers)
33% identity, 94% coverage: 2:328/347 of query aligns to 76:408/551 of P35520
- P78 (≠ N4) to R: in CBSD; severe form; associated in cis with N-102; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs786204608
- G85 (= G11) to R: in CBSD; loss of cystathionine beta-synthase activity; dbSNP:rs863223435
- T87 (= T13) to N: in CBSD; decreased cystathionine beta-synthase activity; increased aggregation
- L101 (vs. gap) to P: in CBSD; common mutation in Irish population; loss of activity; dbSNP:rs786204757
- K102 (vs. gap) to N: in CBSD; associated in cis with R-78; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs786204609; to Q: in dbSNP:rs34040148
- C109 (≠ I31) to R: in CBSD; loss of activity; dbSNP:rs778220779
- A114 (≠ P36) to V: in CBSD; mild form; when linked with W-58 severe form; decreased cystathionine beta-synthase activity; decreases homotetramer formation by promoting formation of larger aggregates; dbSNP:rs121964964
- K119 (= K41) modified: N6-(pyridoxal phosphate)lysine
- R125 (≠ N47) to Q: in CBSD; severe form; when linked with D-131 moderate form; loss of cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs781444670; to W: in CBSD; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; dbSNP:rs886057100
- M126 (= M48) to V: in CBSD; loss of activity
- E131 (≠ L53) to D: in CBSD; linked with Q-125; loss of activity; dbSNP:rs1555875351
- G139 (= G61) to R: in CBSD; mild form; dbSNP:rs121964965
- I143 (≠ V65) to M: in CBSD; 4% of activity; stable; dbSNP:rs370167302
- E144 (= E66) to K: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs121964966
- G148 (= G70) to R: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; loss of homotetramer formation; dbSNP:rs755952006
- N149 (= N71) binding
- L154 (= L76) to Q: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity
- A155 (= A77) to T: in CBSD; complete loss of activity; severely affects homotetramer formation by promoting formation of larger aggregates; dbSNP:rs1429138569; to V: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity
- C165 (≠ F87) to Y: in CBSD; severe form; protein expression is comparable to wild-type; loss of cystathionine beta-synthase activity; no effect on homotetramer formation; dbSNP:rs1347651454
- M173 (≠ A95) to V: in CBSD; presents 40% of the wild-type activity; highly reduced capacity to form multimeric quaternary structure; natural variant: Missing (in CBSD; loss of activity)
- E176 (≠ D98) to K: in CBSD; severe form; loss of cystathionine beta-synthase activity; inhibited by AdoMet; severely decreases homotetramer formation by promoting formation of larger aggregates; dbSNP:rs762065361
- V180 (= V102) to A: in CBSD; decreased cystathionine beta-synthase activity; decreases homotetramer formation; dbSNP:rs1555875010
- T191 (≠ V113) to M: in CBSD; moderate and severe forms; loss of cystathionine beta-synthase activity; absent capacity to form multimeric quaternary structure; dbSNP:rs121964973
- K211 (≠ A135) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
- A226 (= A150) to T: in CBSD; presents 20% of the wild-type activity; dramatically reduced capacity to form multimeric quaternary structure; dbSNP:rs763835246
- N228 (= N152) to K: in CBSD; loss of cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs1464223176; to S: in CBSD; has significantly decreased levels of enzyme activity; dbSNP:rs1555874803
- A231 (≠ G154) to P: in CBSD; has significantly decreased levels of enzyme activity
- D234 (≠ S157) to N: in CBSD; decreased cystathionine beta-synthase activity; changed localization; decreased interaction with pyridoxal 5'-phosphate; no effect on homotetramer formation; dbSNP:rs773734233; natural variant: Missing (in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity)
- GTGGT 256:260 (≠ GTGGS 179:183) binding
- T257 (= T180) to M: in CBSD; moderate to severe form; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs758236584
- T262 (= T185) to M: in CBSD; moderate form; dbSNP:rs149119723; to R: in CBSD; severe form; loss of cystathionine beta-synthase activity; loss of homotetramer formation
- R266 (= R189) to K: in CBSD; mild form; decreased cystathionine beta-synthase activity; decreased homotetramer formation; no effect on heme-binding; decreased stability; dbSNP:rs121964969
- K269 (= K192) natural variant: Missing (in CBSD; loss of expression)
- C272 (≠ N195) mutation to A: Reduced heme content and cystathionine beta-synthase activity.
- C275 (≠ T198) to Y: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; mutation to S: Reduced heme content and cystathionine beta-synthase activity.
- I278 (≠ V201) to S: in CBSD; loss of activity; to T: in CBSD; mild to severe form; common mutation; decreased expression; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; severely affects homotetramer formation by promoting formation of larger aggregates; dbSNP:rs5742905
- D281 (≠ E204) to N: in CBSD; loss of activity
- A288 (≠ F211) to T: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs141502207
- E302 (≠ T225) to K: in CBSD; no effect on cystathionine beta-synthase activity; inhibited by AdoHcy and impaired activation by AdoMet; no effect on homotetramer formation; dbSNP:rs779270933
- G305 (= G228) to R: in CBSD; loss of cystathionine beta-synthase activity; no effect on homotetramer formation
- G307 (vs. gap) to S: in CBSD; moderate to severe form; linked with D-534; common mutation; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; no effect on homotetramer formation; dbSNP:rs121964962
- V320 (≠ I240) to A: in CBSD; has 36% of wild-type enzyme activity; dbSNP:rs781567152
- D321 (= D241) to V: in CBSD; loss of activity
- R336 (= R256) to C: in CBSD; protein expression is comparable to wild-type; loss of activity; absent capacity to form multimeric quaternary structure; dbSNP:rs398123151; to H: in CBSD; mild form; no effect on expression; exhibits an activity lower than 4% of the wild-type enzyme; altered stimulation by AdoMet; absent capacity to form multimeric quaternary structure; dbSNP:rs760417941
- L338 (≠ I258) to P: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
- G347 (= G267) to S: in CBSD; protein expression is comparable to wild-type; loss of activity; dbSNP:rs771298943
- S349 (= S269) binding ; to N: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
- T353 (≠ A273) to M: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs121964972
- R369 (≠ N289) to C: in CBSD; when linked with C-491 severe form; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs117687681
- D376 (= D296) to N: in CBSD; has significantly decreased levels of enzyme activity; dbSNP:rs1170128038
- R379 (≠ E299) to Q: in CBSD; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; dbSNP:rs763036586
- K384 (≠ T304) to E: in CBSD; severe form; dbSNP:rs121964967
Sites not aligning to the query:
- 18 R → C: associated with 1/3 to 2/3 the enzyme activity of the wild-type; dbSNP:rs201827340
- 49 P → L: in CBSD; decreased expression; no effect on cystathionine beta-synthase activity; increased homotetramer formation; dbSNP:rs148865119
- 52 binding axial binding residue
- 58 R → W: in CBSD; linked with V-114; 18% of activity; dbSNP:rs555959266
- 65 binding axial binding residue; H → R: in CBSD; decreased cystathionine beta-synthase activity; inhibited by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs1191141364
- 69 A → P: in dbSNP:rs17849313
- 422 P → L: in CBSD; changed cystathionine beta-synthase activity; impaired stimulation by AdoMet; does not affect homotetramer formation; dbSNP:rs28934892
- 427 P → L: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; dbSNP:rs863223434
- 435 I → T: in CBSD; no effect on cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; does not affect homotetramer formation
- 439 R → Q: in CBSD; no effect on cystathionine beta-synthase activity; increased homotetramer formation; dbSNP:rs756467921
- 444 D → N: in CBSD; decreased expression; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; increased homotetramer formation; dbSNP:rs28934891
- 449 V → G: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet
- 456 L → P: in CBSD; severe; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
- 466 S → L: in CBSD; increased cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs121964971
- 500 S → L: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; dbSNP:rs755106884
- 526 Q → K: in CBSD; has significantly decreased levels of enzyme activity
- 539 L → S: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; loss of homotetramer formation; dbSNP:rs121964968
- 540 L → Q: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet
- 548 R → Q: presents 60% of the wild-type activity; highly reduced capacity to form multimeric quaternary structure; dbSNP:rs150828989
4pcuA Crystal structure of delta516-525 e201s human cystathionine beta- synthase with adomet (see paper)
33% identity, 92% coverage: 2:319/347 of query aligns to 34:361/486 of 4pcuA
- active site: K77 (= K41), S105 (= S69), D237 (≠ E204), S305 (= S269)
- binding protoporphyrin ix containing fe: A182 (= A150), P185 (≠ A153), L186 (vs. gap), Y189 (= Y156), R222 (= R189), T269 (≠ L233)
- binding pyridoxal-5'-phosphate: K77 (= K41), N107 (= N71), S210 (≠ C177), G212 (= G179), T213 (= T180), G214 (= G181), G215 (= G182), T216 (≠ S183), E260 (≠ A227), G261 (= G228), I262 (vs. gap), S305 (= S269), P331 (≠ A295), D332 (= D296)
Sites not aligning to the query:
- binding protoporphyrin ix containing fe: 8, 9, 10, 11, 12, 16, 20, 21, 22, 23
- binding s-adenosylmethionine: 375, 376, 377, 396, 396, 397, 398, 398, 399, 474, 476, 478, 479
6c2zA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the plp-aminoacrylate intermediate (see paper)
34% identity, 93% coverage: 4:325/347 of query aligns to 9:345/345 of 6c2zA
- binding calcium ion: N180 (vs. gap), D183 (≠ G169), N184 (≠ K170)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K50 (= K41), T78 (≠ S68), S79 (= S69), G80 (= G70), N81 (= N71), T82 (= T72), Q154 (= Q146), G191 (≠ C177), A192 (≠ V178), G193 (= G179), T194 (= T180), G195 (= G181), G196 (= G182), T197 (≠ S183), E241 (≠ S221), G242 (= G222), I243 (≠ T223), S286 (= S269), P315 (≠ A295), D316 (= D296)
6c2qA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the plp-l-serine intermediate (see paper)
34% identity, 93% coverage: 4:325/347 of query aligns to 9:345/345 of 6c2qA
- binding calcium ion: N180 (vs. gap), D183 (≠ G169), N184 (≠ K170)
- binding L-Serine, N-[[3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]-4-pyridinyl]methylene]: K50 (= K41), T78 (≠ S68), S79 (= S69), G80 (= G70), N81 (= N71), T82 (= T72), Q154 (= Q146), A192 (≠ V178), G193 (= G179), T194 (= T180), G195 (= G181), G196 (= G182), T197 (≠ S183), E241 (≠ S221), G242 (= G222), I243 (≠ T223), Y245 (≠ T225), S286 (= S269), P315 (≠ A295), D316 (= D296)
6c2hA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the catalytic core (see paper)
34% identity, 93% coverage: 4:325/347 of query aligns to 9:345/345 of 6c2hA
- binding calcium ion: N180 (vs. gap), D183 (≠ G169), N184 (≠ K170)
- binding pyridoxal-5'-phosphate: K50 (= K41), N81 (= N71), G191 (≠ C177), A192 (≠ V178), G193 (= G179), T194 (= T180), G195 (= G181), G196 (= G182), T197 (≠ S183), E241 (≠ S221), G242 (= G222), I243 (≠ T223), S286 (= S269), P315 (≠ A295), D316 (= D296)
6c4pA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the pmp complex (see paper)
34% identity, 93% coverage: 4:325/347 of query aligns to 8:344/344 of 6c4pA
- binding calcium ion: N179 (vs. gap), D182 (≠ G169), N183 (≠ K170)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: K49 (= K41), S78 (= S69), N80 (= N71), G190 (≠ C177), A191 (≠ V178), G192 (= G179), T193 (= T180), G194 (= G181), G195 (= G182), T196 (≠ S183), E240 (≠ S221), G241 (= G222), I242 (≠ T223), S285 (= S269), P314 (≠ A295), D315 (= D296)
5ohxA Structure of active cystathionine b-synthase from apis mellifera (see paper)
34% identity, 92% coverage: 10:328/347 of query aligns to 39:359/488 of 5ohxA
- binding protoporphyrin ix containing fe: T179 (≠ D148), P181 (≠ A150), P184 (≠ A153), L185 (vs. gap), Y188 (= Y156), R221 (= R189), T264 (≠ L233), V265 (= V234)
- binding pyridoxal-5'-phosphate: K74 (= K41), N104 (= N71), G209 (≠ C177), A210 (≠ V178), G211 (= G179), T212 (= T180), G213 (= G181), G214 (= G182), T215 (≠ S183), G256 (= G222), S300 (= S269), P326 (≠ A295), D327 (= D296)
Sites not aligning to the query:
- binding protoporphyrin ix containing fe: 4, 6, 7, 8, 9, 10, 14, 16, 17, 18, 19
Q2V0C9 Cystathionine beta-synthase; EC 4.2.1.22 from Apis mellifera (Honeybee) (see paper)
34% identity, 92% coverage: 10:328/347 of query aligns to 43:366/504 of Q2V0C9
- K78 (= K41) modified: N6-(pyridoxal phosphate)lysine
- N108 (= N71) binding
- GTGGT 215:219 (≠ GTGGS 179:183) binding
- S307 (= S269) binding
Sites not aligning to the query:
- 12 binding axial binding residue
- 23 binding axial binding residue
7xoyA Cystathionine beta-synthase of mycobacterium tuberculosis in the presence of s-adenosylmethionine and serine. (see paper)
37% identity, 91% coverage: 2:317/347 of query aligns to 1:315/458 of 7xoyA
- binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: K42 (= K41), S70 (= S69), G71 (= G70), N72 (= N71), T73 (= T72), H155 (≠ G155), G177 (≠ C177), G179 (= G179), T180 (= T180), G181 (= G181), G182 (= G182), T183 (≠ S183), S267 (= S269), P293 (≠ A295), D294 (= D296)
7xnzB Native cystathionine beta-synthase of mycobacterium tuberculosis. (see paper)
37% identity, 91% coverage: 2:317/347 of query aligns to 1:315/458 of 7xnzB
- binding pyridoxal-5'-phosphate: K42 (= K41), N72 (= N71), G179 (= G179), T180 (= T180), G181 (= G181), G182 (= G182), T183 (≠ S183), E222 (≠ S221), G223 (= G222), V224 (≠ T223), S267 (= S269), P293 (≠ A295), D294 (= D296)
P9WP51 Probable cystathionine beta-synthase Rv1077; Beta-thionase; Serine sulfhydrase; EC 4.2.1.22 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
37% identity, 91% coverage: 2:317/347 of query aligns to 3:317/464 of P9WP51