SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO356_28845 FitnessBrowser__pseudo5_N2C3_1:AO356_28845 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
31% identity, 92% coverage: 2:231/251 of query aligns to 10:247/265 of P07821

query
sites
P07821

V

T

T

T

I

F

R

A

L

L

E

R

N

N

L

I

G

S

A

F

R

R

Y

V

G

P

Q

G

R

R

T

T

V

L

I

L

H

H

G

P

V

L

T

S

T

L

A

T

A

-

F

F

V

P

G

A

G

G

Q

K

V

V

V

T

A

G

V

L

V

I

G

G

P

H

N

N

A

G

A

S

G

G

K
|
K

S

S

T

T

L

L

F

L

K

K

R

-

M

M

A

L

G

G

L

R

I

H

D

Q

G

P

P

P

-

S

-

E

G

G

Q

E

V

I

I

L

L

L

E

D

G

A

-

Q

-

P

-

L

-

E

-

S

-

W

-

S

S

S

K

K

K

A

G

F

P

A

Q

R

G

K

I

V

S

A

Y

Y

M

L

P

P

Q

Q

G

Q

L

L

N

P

A

P

S

A

A

E

R

G

L

M

T

T

V

V

Y

R

E

E

S

L

V

V

L

A

L

I

A

G

R

R

K

Y

Q

P

L

W

T

H

P

G

G

A

-

L

-

G

-

R

W

F

V

G

V

A

H

A

D

D

D

R

E

E

L

-

K

-

R

K

V

V

D

E

I

A

L

I

A

S

A

L

L

V

G

G

I

L

T

K

E

P

L

L

S

A

F

H

R

R

N

L

L

V

G

D

E

S

L

L

S

S

G

G

Q

E

Q

R

Q

Q

L

R

V

A

S

W

I

I

A

A

Q

M

T

L

L

V

V

A

R

Q

E

D

P

S

E

R

I

C

L

L

M

L

D
|
D

E
|
E

P

P

T

T

S

S

A

A

L

L

D

D

M

I

H

A

R

H

Q

Q

V

V

Q

D

V

V

L

L

N

S

F

L

M

V

R

H

A

R

L

L

A

S

R

Q

K

E

R

R

Q

G

V

L

I

T

V

V

F

I

I

A

A

V

I

L

H

H

D

D

L

I

N

N

Q

M

A

A

L

A

R

R

F

Y

A

C

D

D

Q

Y

V

L

L

V

V

A

I

L

A

R

D

G

N

G

T

E

A

M

Q

I

G

A

S

Q

G

G

P

T

S

P

A

A

E

E

V

I

I

M

T

R

E

G

S

E

M

T

L

L

R

E

Q

M

V

I

Y

Y

K

G

V

I

K50 (= K43) mutation to Q: Lack of activity.
D172 (= D156) mutation to E: Lack of activity.
E173 (= E157) mutation to A: Lack of activity.

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
32% identity, 82% coverage: 1:205/251 of query aligns to 4:202/353 of 1vciA

query
sites
1vciA

M

M

V

V

T

E

I

V

R

K

L

L

E

E

N

N

L

L

G

T

A

K

R

R

Y
x
F

G

G

Q

N

R
x
F

T

T

V

A

I

V

H

N

G

K

V

L

T

N

T

-

A

L

A

T

F

I

V

K

G

D

G

G

Q

E

V

F

V

L

A

V

V

L

G

G

P

P

N
x
S

A
x
G

A

C

G
|
G

K
|
K

S
x
T

T
|
T

L

T

F

L

K

R

R

M

M

I

A

A

G

G

L

L

I

E

D

E

-

P

G

T

P

E

G

G

Q

R

V

I

I

Y

L

F

E

-

G

-

S

-

K

-

G

G

P

D

Q

R

G

D

I

V

S

T

Y

Y

M

L

P

P

-

P

Q

K

G

D

L

R

N

N

A

I

S

S

A

M

-

V

-

F

-

Q

R

H

L

M

T

T

V

V

Y

Y

E

E

S

N

V

I

L

A

L

F

A

P

R

L

K

K

Q

K

L

F

T

P

P

-

G

-

W

-

V

-

H

K

D

D

D

E

E

I

L

D

K

K

R

R

V

V

D

R

D

W

I

A

L

A

A

E

A

L

L

L

G

Q

I

I

T

E

E

E

L

L

S

L

F

N

R

R

N

Y

L

P

G

A

E

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

L

R

V

V

S

A

I

V

A

A

Q

R

T

A

L

I

V

V

R

V

E

E

P

P

E

D

I

V

L

L

M

M

D

D

E

E

P

P

T

L

S

S

A

N

L

L

D

D

M

A

H

K

R

L

Q

R

V

V

Q

A

V

M

L

R

N

A

F

E

M

I

R

K

A

K

L

L

A

Q

R

Q

K

K

R

L

Q

K

V

V

I

T

V

T

F

I

I

Y

A

V

I

T

H

H

D

D

L

Q

N

V

Q

E

A

A

L

M

R

T

F

M

A

G

D

D

Q

R

V

I

L

A

V

V

I

M

binding adenosine-5'-triphosphate: F16 (≠ Y13), F19 (≠ R16), S41 (≠ N39), G42 (≠ A40), G44 (= G42), K45 (= K43), T46 (≠ S44), T47 (= T45)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
32% identity, 86% coverage: 4:220/251 of query aligns to 2:224/240 of 4ymuJ

query
sites
4ymuJ

I

I

R

F

L

V

E

N

N

D

L

V

G

Y

A

K

R

N

Y
x
F

G

G

Q

S

R

L

T

E

V
|
V

I

L

H

K

G

G

V

V

T

T

T

L

A

K

A

V

F

N

V

-

G

K

G

G

Q

E

V

V

A

V

V

I

G

G

P

P

N
x
S

A
x
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

F

L

K

-

R

R

M

C

A

I

G

N

L

L

I

L

D

E

G

E

P

P

-

T

-

K

G

G

Q

E

V

V

I

F

L

I

E

D

G

G

S

V

K

K

-

I

-

N

-

G

-

K

-

V

-

N

-

I

-

N

K

K

G

V

P

R

Q

Q

G

K

I

V

S

G

Y

M

M

V

P

F

Q

Q

G

H

L

F

N

N

A

L

S

F

A

P

R

H

L

L

T

T

V

A

Y

I

E

E

S

N

V

I

L

T

L

L

A

A

R

P

K

V

Q

K

L

V

T

K

P

K

G

M

W

N

V

K

H

E

D

A

D

E

E

E

L

L

K

-

R

-

V

A

D

V

D

D

I

L

L

L

A

A

A

K

L

V

G

G

I

L

T

L

E

D

L

K

S

K

F

D

R

Q

N

Y

L

P

G

I

E

K

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

L

R

V

L

S

A

I

I

A

A

Q

R

T

A

L

L

V

A

R

M

E

Q

P

P

E

E

I

V

L

M

L

L

M

F

D

D

E
|
E

P

P

T

T

S

S

A

A

L

L

D

D

M

P

H

E

R

M

Q

V

V

K

Q

E

V

V

L

L

N

N

F

V

M

M

R

K

A

Q

L

L

A

A

R

N

K

E

R

G

Q

M

V

T

I

M

V

V

F

V

I

V

A

T

I

-

H
|
H

D

E

L

M

N

G

Q

F

A

A

L

R

R

E

F

V

A

G

D

D

Q

R

V

V

L

I

V

F

I

M

A

D

D

D

N

G

T

V

A

I

Q

V

G

E

S

E

G

G

P

T

S

P

A

E

E

E

V

I

I

F

binding adenosine-5'-triphosphate: F11 (≠ Y13), V16 (= V18), S36 (≠ N39), G37 (≠ A40), S38 (≠ A41), G39 (= G42), K40 (= K43), S41 (= S44), T42 (= T45), E162 (= E157), H194 (= H190)
binding magnesium ion: S41 (= S44), E162 (= E157)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
33% identity, 87% coverage: 4:221/251 of query aligns to 3:225/241 of 4u00A

query
sites
4u00A

I

I

R

R

L

I

E

R

N

N

L

L

G

H

A

K

R

W

Y
x
F

G

G

Q

P

R

L

T

H

V
|
V

I

L

H

K

G

G

V

I

T

H

T

L

A

E

A

V

F

A

V

P

G

G

G

E

Q

K

V

L

V

V

A

-

V

I

G

G

P

P

N
x
S

A
x
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

F

I

K

R

R

T

M

I

A

N

G

R

L

L

I

E

D

D

-

F

G

Q

P

E

G

G

Q

E

V

V

I

V

L

V

E

D

G

G

-

L

S

S

K

V

K

K

G

D

P

D

Q

R

G

A

-

L

-

R

-

E

-

I

-

R

-

E

I

V

S

G

Y

M

M

V

P

F

Q

Q

G

Q

L

F

N

N

A

L

S

F

A

P

R

H

L

M

T

T

V

V

Y

L

E

E

S

N

V

V

L

T

L

L

A

A

R

P

K

M

Q

R

L

V

T

R

P

-

G

R

W

W

V

P

V

R

H

E

D

K

D

A

E

E

L

-

K

K

R

K

V

A

D

L

D

E

I

L

L

L

A

E

A

R

L

V

G

G

I

I

T

L

E

D

L

Q

S

A

F

R

R

K

N

Y

L

P

G

A

E

Q

L

L

S

S

G

G

Q

Q

L

R

V

V

S

A

I

I

A

A

Q

R

T

A

L

L

V

A

R

M

E

E

P

P

E

K

I

I

L

M

L

L

M

F

D

D

E

E

P

P

T

T

S

S

A

A

L

L

D

D

M

P

H

E

R

M

Q

V

V

G

Q

E

V

V

L

L

N

D

F

V

M

M

R

R

A

D

L

L

A

A

R

Q

K

G

R

G

Q

M

V

T

I

M

V

V

F

V

I

V

A

T

I

-

H

H

D

E

L

M

N

G

Q

F

A

A

L

R

R

E

F

V

A

A

D

D

Q

R

V

V

L

V

V

F

I

M

A

D

D

G

N

G

T

Q

A

I

Q

V

G

E

S

E

G

G

P

R

S

P

A

E

E

E

V

I

I

F

T

T

binding adenosine-5'-diphosphate: F12 (≠ Y13), V17 (= V18), S37 (≠ N39), G38 (≠ A40), S39 (≠ A41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45)

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
28% identity, 87% coverage: 1:219/251 of query aligns to 4:230/375 of 2d62A

query
sites
2d62A

M

M

V

A

T

E

I

V

R

K

L

L

E

I

N

N

L

I

G

W

A

K

R

R

Y

F

G

G

Q

D

R

V

T

T

V

A

I

V

H

K

G

D

V

L

T

S

T

L

A

E

A

-

F

I

V

K

G

D

G

G

Q

E

V

F

V

L

A

V

V

L

G

G

P

P

N

S

A
x
G

A
x
C

G
|
G

K
|
K

S
x
T

T
|
T

L

T

F

L

K

R

R

M

M

I

A

A

G

G

L

L

I

E

D

E

G

P

P

T

-

R

G

G

Q

Q

V

I

I

Y

L

I

E

E

G

D

S

N

-

L

K

V

K

A

G

D

P

P

Q

E

G

K

I

G

S

V

Y

F

M

V

P

P

-

P

-

K

-

E

-

R

-

D

-

V

-

A

-

M

-

V

-

F

Q

Q

G

S

L

Y

N

A

A

L

S

Y

A

P

R

H

L

M

T

T

V

V

Y

Y

E

D

S

N

V

I

L

A

L

F

A

P

R

L

K

K

-

L

Q

R

L

K

T

V

P

P

G

K

W

Q

V

E

V

I

H

D

D

-

E

-

L

-

K

K

R

R

V

V

D

R

D

E

I

V

L

A

A

E

A

M

L

L

G

G

I

L

T

T

E

E

L

L

S

L

F

N

R

R

N

K

L

P

G

R

E

E

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

L

R

V

V

S

A

I

L

A

G

Q

R

T

A

L

I

V

I

R

R

E

R

P

P

E

K

I

V

L

F

L

L

M

M

D

D

E

E

P

P

T

L

S

S

A

N

L

L

D

D

M

A

H

K

R

L

Q

R

V

V

Q

K

V

M

L

R

N

A

F

E

M

L

R

K

A

K

L

L

A

Q

R

R

K

Q

R

L

Q

G

V

V

I

T

V

T

F

I

I

Y

A

V

I

T

H

H

D

D

L

Q

N

V

Q

E

A

A

L

M

R

T

F

M

A

G

D

D

Q

R

V

I

L

A

V

V

I

M

A

N

D

K

N

G

T

E

A

L

Q

Q

G

Q

S

V

G

G

P

T

S

P

A

D

E

E

V

V

binding pyrophosphate 2-: G42 (≠ A40), C43 (≠ A41), G44 (= G42), K45 (= K43), T46 (≠ S44), T47 (= T45)

4zirB Crystal structure of ecfaa' heterodimer bound to amppnp (see paper)
31% identity, 86% coverage: 4:220/251 of query aligns to 4:216/247 of 4zirB

query
sites
4zirB

I

I

R

E

L

L

E

N

N

S

L

V

G

S

A

F

R

R

Y
|
Y

G

N

Q

G

R

D

T

Y

V
|
V

I

L

H

K

G

D

V

V

T

N

T

-

A

A

A

E

F

F

V

E

G

T

G

G

Q

K

V

I

V

Y

A

V

V

V

G

G

P

K

N
|
N

A
x
G

A

S

G
|
G

K
|
K

S
x
T

T
|
T

L

L

F

L

K

K

R

I

M

L

A

A

G

G

L

L

I

L

D

A

G

A

P

A

G

G

Q

E

V

I

I

F

L

L

E

D

G

G

S

S

K

P

K

A

G

D

P

P

-

F

-

L

-

R

Q

K

G

N

I

V

S

G

Y

Y

M

V

P

F

Q
|
Q

G

-

L

-

N

N

A

P

S

S

A

S

R

Q

L

I

-

I

-

G

-

A

T

T

V

V

Y

E

E

E

S

D

V

V

L

A

L

F

A

S

R

L

K

E

Q

-

L

-

T

-

P

-

G

-

W

-

V

-

V

I

H

D

D

E

D

S

E

E

L

M

-

R

K

K

R

R

V

I

D

K

I

V

L

L

A

E

A

L

L

V

G

G

I

L

T

S

E

G

L

L

S

A

F

A

R

A

N

D

L

P

G

L

E
x
N

L

L

S
|
S

G
|
G

Q

Q

Q

K

Q

Q

L

R

V

L

S

A

I

I

A

A

Q

S

T

M

L

L

V

A

R

R

E

D

P

T

E

R

I

F

L

L

L

A

M

L

D

D

E

E

P

P

T

V

S

S

A

M

L

L

D

D

M

P

H

P

R

S

Q

Q

V

R

Q

E

V

I

L

F

N

Q

F

V

M

L

R

E

A

S

L

L

A

K

R

N

K

E

R

G

Q

K

V

G

I

I

V

I

F

L

I

V

A

T

I

-

H

H

D

E

L

L

N

-

Q

E

A

Y

L

L

R

D

F

D

A

M

D

D

Q

F

V

I

L

L

V

H

I

I

A

S

D

N

N

G

T

T

A

I

Q

D

G

F

S

C

G

G

P

S

S

W

A

E

E

E

V

F

I

V

binding phosphoaminophosphonic acid-adenylate ester: Y13 (= Y13), V18 (= V18), N38 (= N39), G39 (≠ A40), G41 (= G42), K42 (= K43), T43 (≠ S44), T44 (= T45), Q80 (= Q77), N129 (≠ E131), S131 (= S133), G132 (= G134), G133 (= G135)
binding magnesium ion: T43 (≠ S44), Q80 (= Q77)

4hluC Structure of the ecfa-a' heterodimer bound to adp (see paper)
31% identity, 86% coverage: 4:220/251 of query aligns to 5:220/249 of 4hluC

query
sites
4hluC

I

I

R

E

L

L

E

N

N

S

L

V

G

S

A

F

R

R

Y
|
Y

G

N

Q

G

R

D

T

Y

V

V

I

L

H

K

G

D

V

V

T

N

T

-

A

A

A

E

F

F

V

E

G

T

G

G

Q

K

V

I

V

Y

A

V

V

V

G

G

P

K

N
|
N

A
x
G

A

S

G
|
G

K
|
K

S
x
T

T
|
T

L

L

F

L

K

K

R

I

M

L

A

A

G

G

L

L

I

L

D

A

G

A

P

A

G

G

Q

E

V

I

I

F

L

L

E

D

G

G

S

S

K

P

K

A

G

D

P

P

-

F

-

L

-

R

Q

K

G

N

I

V

S

G

Y

Y

M

V

P

F

Q

Q

G

-

L

-

N

N

A

P

S

S

A

S

R

Q

L

I

-

I

-

G

-

A

T

T

V

V

Y

E

E

E

S

D

V

V

L

A

L

F

A

S

R

L

K

E

Q

I

L

M

T

G

P

-

G

-

W

-

V

-

V

L

H

D

D

E

D

S

E

E

L

M

-

R

K

K

R

R

V

I

D

K

I

V

L

L

A

E

A

L

L

V

G

G

I

L

T

S

E

G

L

L

S

A

F

A

R

A

N

D

L

P

G

L

E

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

L

R

V

L

S

A

I

I

A

A

Q

S

T

M

L

L

V

A

R

R

E

D

P

T

E

R

I

F

L

L

L

A

M

L

D

D

E

E

P

P

T

V

S

S

A

M

L

L

D

D

M

P

H

P

R

S

Q

Q

V

R

Q

E

V

I

L

F

N

Q

F

V

M

L

R

E

A

S

L

L

A

K

R

N

K

E

R

G

Q

K

V

G

I

I

V

I

F

L

I

V

A

T

I

-

H

H

D

E

L

L

N

-

Q

E

A

Y

L

L

R

D

F

D

A

M

D

D

Q

F

V

I

L

L

V

H

I

I

A

S

D

N

N

G

T

T

A

I

Q

D

G

F

S

C

G

G

P

S

S

W

A

E

E

E

V

F

I

V

binding adenosine-5'-diphosphate: Y14 (= Y13), N39 (= N39), G40 (≠ A40), G42 (= G42), K43 (= K43), T44 (≠ S44), T45 (= T45)

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
30% identity, 77% coverage: 29:222/251 of query aligns to 42:238/378 of P69874

query
sites
P69874

G

N

G

G

Q

E

V
x
F

V

L

A

T

V

L

G

G

P

P

N

S

A

G

A
x
C

G

G

K

K

S

T

T

T

L

V

F
x
L

K

R

R

L

M

I

A

A

G

G

L

L

-

E

-

T

I

V

D

D

G

S

P

-

G

G

Q

R

V

I

I

M

L
|
L

E

D

G

N

S

-

K

-

G

-

P

-

Q

E

G

D

I

I

S

T

Y

H

M

V

P

P

-

A

-

E

-

N

-

R

-

Y

-

V

-

N

-

T

-

V

-

F

Q

Q

G

S

L

Y

N

A

A

L

S

F

A

P

R

H

L

M

T

T

V

V

Y

F

E

E

S

N

V

V

L

A

L

F

A

G

-

L

R

R

K

M

Q

Q

L

K

T

T

P

P

G

A

W

A

V

E

V

I

H

-

D

-

E

-

L

T

K

P

R

R

V

V

D

M

D

E

I

A

L

L

A

R

A

M

L
x
V

G

Q

I

L

T

E

E

T

L

F

S

A

F

Q

R

R

N

K

L

P

G

H

E

Q

L

L

S

S

G

G

Q

Q

L

R

V

V

S

A

I

I

A

A

Q

R

T

A

L

V

V

V

R

N

E

K

P

P

E

R

I

L

L

L

M

L

D
|
D

E

E

P

S

T

L

S

S

A

A

L

L

D

D

M

Y

H

K

R

L

Q

R

V

K

Q

Q

V

M

L

Q

N

N

F

E

M

L

R

K

A

A

L

L

A

Q

R

R

K

K

R

L

Q

G

V

I

I

T

V

F

F

V

I

F

A

V

I

T

H

H

D

D

L

Q

N

E

Q

E

A

A

L

L

R

T

F

M

A

S

D

D

Q

R

V

I

L

V

V

V

I

M

A

R

D

D

N

G

T

R

A

I

Q

E

G

Q

S

D

G

G

P

T

S

P

A

R

E

E

V

I

I

Y

T

E

E

E

F45 (≠ V32) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ A41) mutation to T: Loss of ATPase activity and transport.
L60 (≠ F47) mutation to F: Lower ATPase activity and transport efficiency.
L76 (= L62) mutation to P: Lower ATPase activity and transport efficiency.
V135 (≠ L119) mutation to M: Loss of ATPase activity and transport.
D172 (= D156) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

26 C→A: Lower ATPase activity and transport efficiency.
27 F→L: Lower ATPase activity and transport efficiency.
276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

3bk7A Structure of the complete abce1/rnaase-l inhibitor protein from pyrococcus abysii (see paper)
33% identity, 70% coverage: 30:205/251 of query aligns to 368:531/593 of 3bk7A

query
sites
3bk7A

G

G

Q

E

V

V

V

I

A

G

V

I

V

V

G

G

P

P

N
|
N

A
x
G

A
x
I

G
|
G

K
|
K

S
x
T

T
|
T

L

F

F

V

K

K

R

M

M

L

A

A

G

G

L

-

I

V

D

E

G

E

P

P

G

-

Q

-

V

-

I

-

L

T

E

E

G

G

S

K

K

V

K

E

G

W

P

D

Q

L

G

T

I

V

S

A

Y

Y

M

K

P

P

Q

Q

G

Y

L

I

N

K

A

A

S

E

A

Y

R

E

L

G

T

T

V

V

Y

Y

E

E

S

-

V

-

L

L

A

S

R

K

-

I

-

D

-

S

K

S

Q

K

L

L

T

N

P

S

G

N

W

F

V

Y

V

K

H

-

D

-

E

-

L

-

K

-

R

-

V

-

D

T

D

E

I

L

L

L

A

K

A

P

L

L

G

G

I

I

T

I

E

D

L

L

S

Y

F

D

R

R

N

N

L

V

G

E

E

D

L

L

S

S

G

G

Q

E

Q

L

Q

Q

L

R

V

V

S

A

I

I

A

A

Q

A

T

T

L

L

V

L

R

R

E

D

P

A

E

D

I

I

L

Y

L

L

M

L

D

D

E

E

P

P

T

S

S

A

A

Y

L

L

D

D

M

V

H

E

R

Q

Q

R

V

L

Q

A

V

V

L

S

N

R

F

A

M

I

R

R

A

H

L

L

A

M

R

E

K

K

R

N

Q

E

V

K

I

T

V

A

F

L

I

V

A

V

I

E

H

H

D

D

L

V

N

L

Q

M

A

I

L

D

R

Y

F

V

A

S

D

D

Q

R

V

L

L

I

V

V

I

F

binding adenosine-5'-diphosphate: N377 (= N39), G378 (≠ A40), I379 (≠ A41), G380 (= G42), K381 (= K43), T382 (≠ S44), T383 (= T45)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 87, 92, 112, 113, 114, 115, 116, 117, 118, 292, 353
binding iron/sulfur cluster: 15, 16, 20, 25, 29, 30, 38, 39, 55, 56, 57, 58, 59, 61, 65, 70

1yqtA Rnase-l inhibitor (see paper)
34% identity, 70% coverage: 30:205/251 of query aligns to 289:452/515 of 1yqtA

query
sites
1yqtA

G

G

Q

E

V

V

V

I

A

G

V

I

V

V

G

G

P

P

N
|
N

A
x
G

A
x
I

G
|
G

K
|
K

S
x
T

T
|
T

L

F

F

V

K

K

R

M

M

L

A

A

G

G

L

-

I

V

D

E

G

E

P

P

G

-

Q

-

V

-

I

-

L

T

E

E

G

G

S

K

K

I

K

E

G

W

P

D

Q

L

G

T

I

V

S

A

Y

Y

M

K

P

P

Q
|
Q

G

Y

L

I

N

K

A

A

S

D

A

Y

R

E

L

G

T

T

V

V

Y

Y

E

E

S

-

V

-

L

L

A

S

R

K

K

I

Q

D

L

A

T

S

P

K

G

-

W

-

V

-

V

L

H

N

D

S

D

N

E

F

L

Y

K

K

R

-

V

-

D

T

D

E

I

L

L

L

A

K

A

P

L

L

G

G

I

I

T

I

E

D

L

L

S

Y

F

D

R

R

N

E

L

V

G

N

E

E

L

L

S

S

G

G

Q

E

Q

L

Q

Q

L

R

V

V

S

A

I

I

A

A

Q

A

T

T

L

L

V

L

R

R

E

D

P

A

E

D

I

I

L

Y

L

L

M

L

D

D

E

E

P

P

T

S

S

A

A

Y

L

L

D

D

M

V

H

E

R

Q

Q

R

V

L

Q

A

V

V

L

S

N

R

F

A

M

I

R

R

A

H

L

L

A

M

R

E

K

K

R

N

Q

E

V

K

I

T

V

A

F

L

I

V

A

V

I

E

H

H

D

D

L

V

N

L

Q

M

A

I

L

D

R

Y

F

V

A

S

D

D

Q

R

V

L

L

M

V

V

I

F

binding adenosine-5'-diphosphate: N298 (= N39), G299 (≠ A40), I300 (≠ A41), G301 (= G42), K302 (= K43), T303 (≠ S44), T304 (= T45)
binding magnesium ion: T303 (≠ S44), Q331 (= Q77)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 8, 13, 33, 34, 36, 37, 38, 39, 274, 277
binding magnesium ion: 38, 90

5yv5A Crystal structure of the complex of archaeal ribosomal stalk protein ap1 and archaeal ribosome recycling factor aabce1. (see paper)
34% identity, 70% coverage: 30:205/251 of query aligns to 292:455/517 of 5yv5A

query
sites
5yv5A

G

G

Q

E

V

V

V

I

A

G

V

I

V

V

G

G

P

P

N
|
N

A
x
G

A
x
I

G
|
G

K
|
K

S
x
T

T
|
T

L

F

F

V

K

K

R

M

M

L

A

A

G

G

L

-

I

V

D

E

G

E

P

P

G

-

Q

-

V

-

I

-

L

T

E

E

G

G

S

K

K

I

K

E

G

W

P

D

Q

L

G

T

I

V

S

A

Y

Y

M

K

P

P

Q
|
Q

G

Y

L

I

N

K

A

A

S

D

A

Y

R

E

L

G

T

T

V

V

Y

Y

E

E

S

-

V

-

L

L

A

S

R

K

K

I

Q

D

L

A

T

S

P

K

G

-

W

-

V

-

V

L

H

N

D

S

D

N

E

F

L

Y

K

K

R

-

V

-

D

T

D

E

I

L

L

L

A

K

A

P

L

L

G

G

I

I

T

I

E

D

L

L

S

Y

F

D

R

R

N

E

L

V

G

N

E

E

L

L

S

S

G

G

Q

E

Q

L

Q

Q

L

R

V

V

S

A

I

I

A

A

Q

A

T

T

L

L

V

L

R

R

E

D

P

A

E

D

I

I

L

Y

L

L

M

L

D

D

E

E

P

P

T

S

S

A

A

Y

L

L

D

D

M

V

H

E

R

Q

Q

R

V

L

Q

A

V

V

L

S

N

R

F

A

M

I

R

R

A

H

L

L

A

M

R

E

K

K

R

N

Q

E

V

K

I

T

V

A

F

L

I

V

A

V

I

E

H

H

D

D

L

V

N

L

Q

M

A

I

L

D

R

Y

F

V

A

S

D

D

Q

R

V

L

L

M

V

V

I

F

binding adenosine-5'-diphosphate: N301 (= N39), G302 (≠ A40), I303 (≠ A41), G304 (= G42), K305 (= K43), T306 (≠ S44), T307 (= T45)
binding magnesium ion: T306 (≠ S44), Q334 (= Q77)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 11, 14, 16, 36, 37, 39, 40, 41, 42, 277, 280
binding magnesium ion: 41, 93
binding : 94, 97, 98, 100, 101, 151, 152, 155

1l7vC Bacterial abc transporter involved in b12 uptake (see paper)
30% identity, 80% coverage: 30:229/251 of query aligns to 25:229/231 of 1l7vC

query
sites
1l7vC

G

G

Q

E

V

I

V

L

A

H

V

L

V

V

G

G

P

P

N
|
N

A
x
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

F

L

K

A

R

R

M

M

A

A

G

G

L

M

I

T

D

S

G

G

P

K

G

G

Q

S

V

I

I

Q

L

F

E

A

G

G

S

Q

K

-

G

-

P

-

Q

-

G

-

I

-

S

-

Y

-

M

-

P

P

Q

L

G

E

L

A

N

W

A

S

S

A

A

T

R

K

L

L

T

A

V

L

Y

H

E

R

S

A

V

Y

L

L

L

S

A

Q

R

Q

K

Q

Q

T

-

P

-

F

L

A

T

T

P

P

G

V

W

W

-

H

-

Y

-

L

V

T

V

L

H

H

D

Q

D

H

E

D

L

K

K

T

R

R

-

T

-

E

-

L

V

L

D

N

D

D

I

V

L

A

A

G

A

A

L

L

G

A

I

L

T

D

E

D

L

K

S

L

F

G

R

R

N

S

L

T

G

N

E

Q

L

L

S

S

G

G

Q

E

Q

W

Q

Q

L

R

V

V

S

R

I

L

A

A

Q

A

T

V

L

V

V

L

R

Q

-

I

-

T

-

P

-

Q

-

A

E

N

P

P

-

A

-

G

E

Q

I

L

L

L

M

L

D

D

E

E

P

P

T

M

S

N

A

S

L

L

D

D

M

V

H

A

R

Q

Q

Q

V

S

Q

A

V

L

L

D

N

K

F

I

M

L

R

S

A

A

L

L

A

C

R

Q

K

Q

R

G

Q

L

V

A

I

I

V

V

F

-

I

M

A

S

I

S

H

H

D

D

L

L

N

N

Q

H

A

T

L

L

R

R

F

H

A

A

D

H

Q

R

V

A

L

W

V

L

I

L

A

K

D

G

N

G

T

K

A

M

Q

L

G

A

S

S

G

G

P

R

S

R

A

E

E

E

V

V

I

L

T

T

E

P

S

P

M

N

L

L

R

A

Q

Q

V

A

Y

Y

binding cyclo-tetrametavanadate: N34 (= N39), G35 (≠ A40), A36 (= A41), G37 (= G42), K38 (= K43), S39 (= S44), T40 (= T45)

6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
26% identity, 90% coverage: 4:229/251 of query aligns to 3:233/240 of 6mjpA

query
sites
6mjpA

I

L

R

K

L

A

E

Q

N

H

L

L

G

A

A

K

R

S

Y

Y

G

K

Q

K

R

R

T

K

V

V

I

V

H

S

G

D

V

V

T

S

T

L

A

Q

A

V

F

-

V

E

G

S

G

G

Q

Q

V

I

V

V

A

G

V

L

G

G

P

P

N

N

A

G

A

A

G

G

K

K

S

T

T

T

L

S

F

F

K

Y

R

M

M

I

A

V

G

G

L

L

-

V

-

A

-

R

-

D

-

E

-

G

-

T

-

I

-

T

I

I

D

D

G

D

P

N

G

D

Q

I

V

S

I

I

L

L

E

P

G

M

S

H

K

S

K

R

G

S

P

R

Q

M

G

G

I

I

S

G

Y

Y

M

L

P

P

Q

Q

G

E

L

A

N

S

A

I

S

F

A

R

R

K

L

L

T

S

V

V

Y

E

E

D

S

N

-

I

-

M

-

A

V

V

L

L

L

Q

A

T

R

R

K

E

Q

E

L

L

T

T

P

-

G

-

W

-

V

-

H

H

D
x
E

D

E

E

R

L

Q

K
x
D

R

K

V

L

D

E

D

D

I

L

L

L

A

E

L

F

G

H

I

I

T

Q

E

H

L

I

S

R

F

K

R

S

N

A

L

G

G

M

E

A

L

L

S

S

G

G

Q

E

Q

R

L

R

V

V

S

E

I

I

A

A

Q

R

T

A

L

L

V

A

R

A

E

N

P

P

E

Q

I

F

L

I

L

L

M

L

D

D

E

Q

P

P

T

F

S

A

A

G

L

V

D

D

M

P

H

I

R

S

Q

V

V

I

Q

D

V

I

L

K

N

K

F

I

M

I

R

E

A

H

L

L

A

-

R

R

K

D

R

R

Q

G

V

L

I

G

V

V

F

L

I

I

A

T

I

D

H

H

D

N

L

V

N

R

Q

E

A

T

L

L

R

D

F

V

A

C

D

E

Q

K

V

A

L

Y

V

I

I

V

A

S

D

Q

N

G

T

R

A

L

Q

I

G

A

S

E

G

G

P

T

S

P

A

Q

E

D

V

V

I

L

T

N

E

N

S

E

M

Q

L

V

R

K

Q

Q

V

V

Y

Y

binding calcium ion: E111 (≠ D106), D115 (≠ K110)

4fi3C Structure of vitamin b12 transporter btucd-f in a nucleotide-bound state (see paper)
29% identity, 82% coverage: 30:234/251 of query aligns to 25:234/248 of 4fi3C

query
sites
4fi3C

G

G

Q

E

V

I

V

L

A

H

V

L

V

V

G

G

P

P

N
|
N

A
x
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

F

L

K

A

R

R

M

M

A

A

G

G

L

M

I

T

D

S

G

G

P

K

G

G

Q

S

V

I

I

Q

L

F

E

A

G

G

S

Q

K

-

G

-

P

-

Q

-

G

-

I

-

S

-

Y

-

M

-

P

P

Q

L

G

E

L

A

N

W

A

S

S

A

A

T

R

K

L

L

T

A

V

L

Y

H

E

R

S

A

V

Y

L

L

L

S

A
x
Q

R

Q

K

Q

Q

T

-

P

-

F

L

A

T

T

P

P

G

V

W

W

-

H

-

Y

-

L

V

T

V

L

H

H

D

Q

D

H

E

D

L

K

K

T

R

R

-

T

-

E

-

L

V

L

D

N

D

D

I

V

L

A

A

G

A

A

L

L

G

A

I

L

T

D

E

D

L

K

S

L

F

G

R
|
R

N

S

L

T

G

N

E
x
Q

L

L

S
|
S

G

G

G
|
G

Q
x
E

Q

W

Q

Q

L

R

V

V

S

R

I

L

A

A

Q

A

T

V

L

V

V

L

R

Q

-

I

-

T

-

P

-

Q

-

A

E

N

P

P

-

A

-

G

E

Q

I

L

L

L

M

L

D

D

E
x
Q

P

P

T

M

S

C

A

S

L

L

D

D

M

V

H

A

R

Q

Q

Q

V

S

Q

A

V

L

L

D

N

K

F

I

M

L

R

S

A

A

L

L

A

S

R

Q

K

Q

R

G

Q

L

V

A

I

I

V

V

F

-

I

M

A

S

I

S

H

H

D

D

L

L

N

N

Q

H

A

T

L

L

R

R

F

H

A

A

D

H

Q

R

V

A

L

W

V

L

I

L

A

K

D

G

N

G

T

K

A

M

Q

L

G

A

S

S

G

G

P

R

S

R

A

E

E

E

V

V

I

L

T

T

E

P

S

P

M

N

L

L

R

A

Q

Q

V

A

Y

Y

K

G

V

M

E

N

A

F

R

R

binding phosphoaminophosphonic acid-adenylate ester: N34 (= N39), G35 (≠ A40), G37 (= G42), K38 (= K43), S39 (= S44), T40 (= T45), R121 (= R127), Q125 (≠ E131), S127 (= S133), G129 (= G135), E130 (≠ Q136), Q158 (≠ E157)
binding magnesium ion: S39 (= S44), Q79 (≠ A94)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 14

7w78A Heme exporter hrtba in complex with mg-amppnp (see paper)
33% identity, 72% coverage: 30:210/251 of query aligns to 34:215/218 of 7w78A

query
sites
7w78A

G

G

Q

E

V

L

V

V

A

A

V

I

V

V

G

G

P

E

N
x
S

A
x
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

F

L

K

S

R

-

M

I

A

A

G

G

L

F

I

L

D

Q

G

E

P

P

-

T

-

S

G

G

Q

T

V

V

I

T

L

L

E

H

G

G

S

A

K

E

-

G

-

L

-

D

-

A

-

T

-

S

K

T

G

R

P

R

Q

E

G

H

I

I

S

G

Y

F

M

V

P

F

Q
|
Q

G

Q

L

P

N

N

A

L

S

L

A

G

R

S

L

L

T

T

V

A

Y

R

E

E

S

Q

V

L

L

L

L

I

A

T

R

D

-

H

-

L

K

R

Q

G

L

I

T

K

P

P

G

-

W

-

V

-

H

-

D

-

E

R

L

K

K

D

R

R

V

A

D

D

D

E

I

L

L

L

A

A

A

R

L

V

G

G

I

L

T

K

E

G

L

L

S

G

F

G

R

R

N

R

L

V

G

A

E

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

L

R

V

V

S

N

I

I

A

A

Q

R

T

A

L

L

V

M

R

G

E

N

P

P

E

Q

I

L

L

L

M

A

D

D

E
|
E

P

P

T

T

S

S

A

A

L

L

D

D

M

A

H

R

R

L

Q

S

V

K

Q

E

V

I

L

V

N

E

F

L

M

L

R

R

A

D

L

V

A

T

R

K

K

E

R

F

Q

A

V

L

I

A

V

T

F

L

I

M

A

V

I

T

H

H

D

D

L

R

N

S

Q

Q

A

-

L

L

R

A

F

Y

A

A

D

D

Q

R

V

F

L

V

V

E

I

M

A

A

D

D

N

G

T

K

A

A

binding phosphoaminophosphonic acid-adenylate ester: S43 (≠ N39), G44 (≠ A40), G46 (= G42), K47 (= K43), S48 (= S44), T49 (= T45), Q88 (= Q77), E163 (= E157)
binding magnesium ion: S48 (= S44), Q88 (= Q77)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 14

7w79A Heme exporter hrtba in complex with mn-amppnp (see paper)
33% identity, 72% coverage: 30:210/251 of query aligns to 34:215/216 of 7w79A

query
sites
7w79A

G

G

Q

E

V

L

V

V

A

A

V

I

V

V

G

G

P

E

N
x
S

A
x
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

F

L

K

S

R

-

M

I

A

A

G

G

L

F

I

L

D

Q

G

E

P

P

-

T

-

S

G

G

Q

T

V

V

I

T

L

L

E

H

G

G

S

A

K

E

-

G

-

L

-

D

-

A

-

T

-

S

K

T

G

R

P

R

Q

E

G

H

I

I

S

G

Y

F

M

V

P

F

Q
|
Q

G

Q

L

P

N

N

A

L

S

L

A

G

R

S

L

L

T

T

V

A

Y

R

E

E

S

Q

V

L

L

L

L

I

A

T

R

D

-

H

-

L

K

R

Q

G

L

I

T

K

P

P

G

-

W

-

V

-

H

-

D

-

E

R

L

K

K

D

R

R

V

A

D

D

D

E

I

L

L

L

A

A

A

R

L

V

G

G

I

L

T

K

E

G

L

L

S

G

F

G

R

R

N

R

L

V

G

A

E

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

L

R

V

V

S

N

I

I

A

A

Q

R

T

A

L

L

V

M

R

G

E

N

P

P

E

Q

I

L

L

L

M

A

D

D

E
|
E

P

P

T

T

S

S

A

A

L

L

D

D

M

A

H

R

R

L

Q

S

V

K

Q

E

V

I

L

V

N

E

F

L

M

L

R

R

A

D

L

V

A

T

R

K

K

E

R

F

Q

A

V

L

I

A

V

T

F

L

I

M

A

V

I

T

H
|
H

D

D

L

R

N

S

Q

Q

A

-

L

L

R

A

F

Y

A

A

D

D

Q

R

V

F

L

V

V

E

I

M

A

A

D

D

N

G

T

K

A

A

binding adenosine-5'-triphosphate: S43 (≠ N39), G44 (≠ A40), S45 (≠ A41), G46 (= G42), K47 (= K43), S48 (= S44), T49 (= T45)
binding manganese (ii) ion: S48 (= S44), Q88 (= Q77), E163 (= E157), H196 (= H190)

Sites not aligning to the query:

P45844 ATP-binding cassette sub-family G member 1; ATP-binding cassette transporter 8; White protein homolog; EC 7.6.2.- from Homo sapiens (Human) (see 6 papers)
32% identity, 77% coverage: 14:206/251 of query aligns to 96:291/678 of P45844

query
sites
P45844

G

G

Q

Y

R

K

T

T

V

L

I

L

H

K

G

G

V

I

T

S

T

-

A

G

A

K

F

F

V

N

G

S

G

G

Q

E

V

L

V

V

A

A

V

I

V

M

G

G

P

P

N

S

A

G

A

A

G

G

K
|
K

S

S

T

T

L

L

F

M

K

N

R

I

M

L

A

A

G

G

L

-

I

-

D

-

G

-

P

-

G

-

Q

-

V

-

I

Y

L

R

E

E

G

T

S

G

K

M

K

K

G

G

P

A

Q

V

G

L

I

I

S

N

Y

G

M

L

P

P

Q

R

G

D

L

L

N

R

A
x
C

S

F

A

R

R

K

L

V

T

S

V

C

Y

Y

-

I

-

M

-

Q

E

D

S

D

V

M

L

L

A

P

R

H

K

L

Q

T

L

V

T

Q

P

E

G

A

W

M

V

M

V

V

-

S

-

A

-

H

-

L

-

K

-

L

-

Q

H

E

D

K

D

D

E

E

L

G

K

R

R

R

-

E

-

M

V

V

D

K

D

E

I

I

L

L

A

T

A

A

L

L

G

G

I

L

T

L

E

S

L

C

S

A

F

N

R

T

N

R

L

T

G

G

E

S

L

L

S

S

G

G

Q

Q

Q

R

Q

K

L

R

V

L

S

A

I

I

A

A

Q

L

T

E

L

L

V

V

R

N

E

N

P

P

E

P

I

V

L

M

L

F

M

F

D

D

E

E

P

P

T

T

S

S

A

G

L

L

D

D

M

S

H

A

R

S

Q

C

V

F

Q

Q

V

V

L

V

N

S

F

L

M

M

R

K

A

G

L

L

A

A

R

Q

K

G

R

G

Q

R

V

S

I

I

V

I

F

-

I

C

A

T

I

I

H

H

D

Q

L

P

N

S

Q

A

A

K

L

L

-

F

R

E

F

L

A

F

D

D

Q

Q

V

L

L

Y

V

V

I

L

A

S

K124 (= K43) mutation to M: Affects ATP binding. Does not affect efflux of 7beta-hydroxycholesterol. Does not affect localization at plasma membrane. Does not affect homodimerization. Decreases cholesterol and phospholipids efflux. Does not affect heretodimerization with ABCG4.
C154 (≠ A81) modified: S-palmitoyl cysteine; mutation to A: No significant effect.

Sites not aligning to the query:

30 modified: S-palmitoyl cysteine; C→A: No significant effect.
315 modified: S-palmitoyl cysteine; mutation C->A,S: Significantly decreases ABCG1-mediated cholesterol efflux.
394 modified: S-palmitoyl cysteine; C→A: No significant effect.
406 modified: S-palmitoyl cysteine; C→A: No significant effect.
491 Y→A: Significantly reduces interaction with CAV1; when associated with A-493. Significantly reduces ABCG1-mediated cholesterol efflux; when associated with A-493.
493 Y→A: Significantly reduces interaction with CAV1; when associated with A-491. Significantly reduces ABCG1-mediated cholesterol efflux; when associated with A-491.
498 Y→A: Significantly reduces interaction with CAV1; when associated with A-499. Significantly reduces ABCG1-mediated cholesterol efflux; when associated with A-499. Affects subcellular location at plasma membrane; when associated with A-499. Affects ABCG1 traffincking to the plasma membrane; when associated with A-499.; Y→F: Does not affect ABCG1-mediated cholesterol efflux; when associated with I-499.; Y→I: Dramatically reduces the ability of ABCG1 to mediate cholesterol efflux; when associated with I-499.; Y→W: Does not affect ABCG1-mediated cholesterol efflux; when associated with I-499.
499 Y→A: Significantly reduces interaction with CAV1; when associated with A-498. Significantly reduces ABCG1-mediated cholesterol efflux; when associated with A-498. Affects subcellular location at plasma membrane; when associated with A-498. Affects ABCG1 traffincking to the plasma membrane; when associated with A-498.; Y→F: Does not affect ABCG1-mediated cholesterol efflux; when associated with I-498.; Y→I: Dramatically reduces the ability of ABCG1 to mediate cholesterol efflux; when associated with I-498.; Y→W: Does not affect ABCG1-mediated cholesterol efflux; when associated with I-498.
668 natural variant: F -> L

7r8eA The structure of human abcg1 e242q complexed with atp (see paper)
31% identity, 82% coverage: 2:206/251 of query aligns to 4:211/548 of 7r8eA

query
sites
7r8eA

V

V

T

N

I

I

R

E

L

F

E

R

N

D

L

L

G

S

A

Y

R

S

Y

V

G

P

Q

E

R

K

T

T

V

L

I

L

H

K

G

G

V

I

T

S

T

-

A

G

A

K

F

F

V

N

G

S

G

G

Q

E

V

L

V

V

A

A

V

I

V

M

G

G

P

P

N
x
S

A
x
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

F

M

K

N

R

I

M

L

A

A

G

G

L

-

I

-

D

-

G

-

P

-

G

-

Q

-

V

-

I

Y

L

R

E

E

G

T

S

G

K

M

K

K

G

G

P

A

Q

V

G

L

I

I

S

N

Y

G

M

L

P

P

Q

R

G

D

L

L

N

R

A

C

S

F

A

R

R

K

L

V

T

S

V

C

Y

Y

-

I

-

M

-
x
Q

E

D

S

D

V

M

L

L

A

P

R

H

K

L

Q

T

L

V

T

Q

P

E

G

A

W

M

V

M

V

V

-

S

-

A

-

H

-

L

-

K

-

L

-

Q

H

E

D

K

D

D

E

E

L

G

K

R

R

R

-

E

-

M

V

V

D

K

D

E

I

I

L

L

A

T

A

A

L

L

G

G

I

L

T

L

E

S

L

C

S

A

F

N

R

T

N

R

L

T

G

G

E
x
S

L

L

S
|
S

G

G

Q
|
Q

Q

R

Q

K

L

R

V

L

S

A

I

I

A

A

Q

L

T

E

L

L

V

V

R

N

E

N

P

P

E

P

I

V

L

M

L

F

M

F

D

D

E
x
Q

P

P

T

T

S

S

A

G

L

L

D

D

M

S

H

A

R

S

Q

C

V

F

Q

Q

V

V

L

V

N

S

F

L

M

M

R

K

A

G

L

L

A

A

R

Q

K

G

R

G

Q

R

V

S

I

I

V

I

F

-

I

C

A

T

I

I

H

H

D

Q

L

P

N

S

Q

A

A

K

L

L

-

F

R

E

F

L

A

F

D

D

Q

Q

V

L

L

Y

V

V

I

L

A

S

binding adenosine-5'-triphosphate: S40 (≠ N39), G41 (≠ A40), G43 (= G42), K44 (= K43), S45 (= S44), T46 (= T45), S136 (≠ E131), S138 (= S133), Q141 (= Q136)
binding magnesium ion: Q84 (vs. gap), Q162 (≠ E157)

Sites not aligning to the query:

binding cholesterol: 474, 520, 524, 528

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
26% identity, 94% coverage: 4:240/251 of query aligns to 5:243/285 of 4yerA

query
sites
4yerA

I

I

R

V

L

V

E

E

N

N

L

L

G

V

A

K

R

K

Y
x
F

G

G

Q

D

R
x
F

T

E

V

A

I

V

H

K

G

G

V

V

T

S

T

F

A

S

A

V

F

-

V

K

G

K

G

G

Q

E

V

I

V

F

A

A

V

F

V

L

G

G

P

P

N
|
N

A
x
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

-

T

-

I

-

H

-

M

-

L

-

T

L

L

F

L

K

K

R

P

M

T

A

S

G

G

L

K

I

A

D

W

G

V

P

A

G

G

Q

H

V

D

I

V

L

L

E

K

G

E

S

P

K

R

K

E

G

V

P

R

Q

R

G

K

I

I

S

G

Y

I

M

V

P

F

Q

Q

G

D

L

Q

N

S

A

L

S

D

A

R

R

E

L

L

T

T

V

A

Y

Y

E

E

S

N

V

M

L

Y

L

I

A

H

R

G

K

K

Q

I

L

Y

T

G

P

Y

G

G

W

G

V

-

H

-

D

E

D

K

E

L

L

K

K

K

R

R

V

I

D

L

D

E

I

L

L

L

A

E

A

F

L

V

G

E

I

L

T

L

E

E

L

F

S

K

F

D

R

K

N

P

L

V

G

K

E
x
T

L
x
F

S
|
S

G

G

Q

M

Q

A

Q

R

L

R

V

L

S

E

I

I

A

A

Q

R

T

S

L

L

V

I

R

H

E

E

P

P

E

E

I

V

L

L

L

F

M

L

D

D

E

E

P

P

T

T

S

I

A

G

L

L

D

D

M

P

H

H

R

T

Q

R

V

A

Q

H

V

M

L

W

N

E

F

Y

M

I

R

S

A

K

L

M

A

K

R

K

K

E

R

H

Q

N

V

M

I

T

V

I

F

F

I

L

A

T

I

T

H

H

D

Y

L

M

N

D

Q

E

A

A

L

E

R

Q

F

L

A

A

D

D

Q

R

V

V

L

A

V

I

I

I

A

D

D

H

N

G

T

K

A

I

Q

I

G

A

S

L

G

G

P

T

S

P

A

T

E

E

V

-

I

L

T

K

E

R

S

M

M

V

L

G

R

K

Q

E

V

I

Y

I

K

Y

V

V

E

R

A

D

R

F

I

I

E

E

Q

S

C

C

S

R

R

K

binding adenosine-5'-diphosphate: F14 (≠ Y13), F17 (≠ R16), N39 (= N39), G40 (≠ A40), G42 (= G42), K43 (= K43), T44 (≠ S44), T45 (= T45), T135 (≠ E131), F136 (≠ L132), S137 (= S133)

7oz1A Cryo-em structure of abcg1 e242q mutant with atp and cholesteryl hemisuccinate bound (see paper)
32% identity, 77% coverage: 14:206/251 of query aligns to 16:211/544 of 7oz1A

query
sites
7oz1A

G

G

Q

Y

R
x
K

T

T

V
x
L

I

L

H

K

G

G

V

I

T

S

T

-

A

G

A

K

F

F

V

N

G

S

G

G

Q

E

V

L

V

V

A

A

V

I

V

M

G

G

P

P

N

S

A

G

A

A

G
|
G

K
|
K

S
|
S

T

T

L

L

F

M

K

N

R

I

M

L

A

A

G

G

L

-

I

-

D

-

G

-

P

-

G

-

Q

-

V

-

I

Y

L

R

E

E

G

T

S

G

K

M

K

K

G

G

P

A

Q

V

G

L

I

I

S

N

Y

G

M

L

P

P

Q

R

G

D

L

L

N

R

A

C

S

F

A

R

R

K

L

V

T

S

V

C

Y

Y

-

I

-

M

-

Q

E

D

S

D

V

M

L

L

A

P

R

H

K

L

Q

T

L

V

T

Q

P

E

G

A

W

M

V

M

V

V

-

S

-

A

-

H

-

L

-

K

-

L

-

Q

H

E

D

K

D

D

E

E

L

G

K

R

R

R

-

E

-

M

V

V

D

K

D

E

I

I

L

L

A

T

A

A

L

L

G

G

I

L

T

L

E

S

L

C

S

A

F

N

R

T

N

R

L

T

G

G

E

S

L

L

S

S

G

G

Q

Q

Q

R

Q

K

L

R

V

L

S

A

I

I

A

A

Q

L

T

E

L

L

V

V

R

N

E

N

P

P

E

P

I

V

L

M

L

F

M

F

D

D

E
x
Q

P

P

T

T

S

S

A

G

L

L

D

D

M

S

H

A

R

S

Q

C

V

F

Q

Q

V

V

L

V

N

S

F

L

M

M

R

K

A

G

L

L

A

A

R

Q

K

G

R

G

Q

R

V

S

I

I

V

I

F

-

I

C

A

T

I

I

H

H

D

Q

L

P

N

S

Q

A

A

K

L

L

-

F

R

E

F

L

A

F

D

D

Q

Q

V

L

L

Y

V

V

I

L

A

S

binding adenosine-5'-triphosphate: K18 (≠ R16), L20 (≠ V18), G43 (= G42), K44 (= K43), S45 (= S44), Q162 (≠ E157)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 15
binding cholesterol hemisuccinate: 335, 335, 367, 431, 431, 434, 435, 438, 438, 439, 441, 463, 466, 466, 467, 525, 526, 527, 529, 530, 531, 532, 533, 535

Query Sequence

>AO356_28845 FitnessBrowser__pseudo5_N2C3_1:AO356_28845
MVTIRLENLGARYGQRTVIHGVTTAAFVGGQVVAVVGPNAAGKSTLFKRMAGLIDGPGQV
ILEGSKKGPQGISYMPQGLNASARLTVYESVLLARKQLTPGWVVHDDELKRVDDILAALG
ITELSFRNLGELSGGQQQLVSIAQTLVREPEILLMDEPTSALDMHRQVQVLNFMRALARK
RQVIVFIAIHDLNQALRFADQVLVIADNTAQGSGPSAEVITESMLRQVYKVEARIEQCSR
GERHILIDDIV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory