SitesBLAST
Comparing AO356_30295 FitnessBrowser__pseudo5_N2C3_1:AO356_30295 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
42% identity, 97% coverage: 9:693/703 of query aligns to 4:687/692 of 6iunB
- active site: A60 (= A65), F65 (= F70), E73 (vs. gap), H77 (≠ S79), G101 (= G103), E104 (= E106), E124 (= E126), G132 (= G134), K248 (≠ E255), S407 (= S414), H428 (= H435), E440 (= E447), N478 (= N485)
- binding nicotinamide-adenine-dinucleotide: G300 (= G307), T301 (= T308), M302 (= M309), E321 (= E328), T322 (≠ A329), Y365 (= Y372), A377 (= A384), V378 (= V385), E380 (= E387), V384 (≠ L391), V388 (= V395), N405 (= N412), S407 (= S414)
Q08426 Peroxisomal bifunctional enzyme; PBE; PBFE; L-bifunctional protein; LBP; Multifunctional enzyme 1; MFE1; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Homo sapiens (Human) (see 5 papers)
38% identity, 99% coverage: 6:702/703 of query aligns to 1:715/723 of Q08426
- E3 (≠ S8) to K: in FRTS3; the mutant is mistargeted to mitochondria; results in impaired mitochondrial oxidative phosphorylation and defects in the transport of fluids across the epithelium of renal proximal tubular cells; dbSNP:rs398124646
- V40 (≠ N44) to G: in dbSNP:rs1062551
- I41 (≠ A45) to R: in dbSNP:rs1062552
- T75 (≠ S79) to I: in dbSNP:rs1062553
- K165 (≠ Q167) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-171; Q-346 and Q-584.
- K171 (≠ Q173) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-346 and Q-584.
- A274 (= A279) to T: in dbSNP:rs2302819
- A325 (≠ L326) to G: in dbSNP:rs1062555
- K346 (≠ V347) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-584.
- K584 (≠ E575) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-346.
- K598 (≠ R588) to T: in dbSNP:rs1042437
- T606 (≠ E596) to P: in dbSNP:rs1042438
5omoA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with with 3s-hydroxy-decanoyl-coa and 3-keto- decanoyl-coa
39% identity, 94% coverage: 15:675/703 of query aligns to 16:689/725 of 5omoA
- active site: A66 (= A65), F71 (= F70), G81 (≠ A83), G105 (= G103), E108 (= E106), P127 (= P125), E128 (= E126), P135 (= P133), G136 (= G134), K254 (≠ E255), S415 (= S414), H436 (= H435), E448 (= E447), N486 (= N485)
- binding (s)-3-hydroxydecanoyl-coa: P25 (= P24), V26 (= V25), A28 (= A27), P31 (= P30), A64 (= A63), A66 (= A65), D67 (= D66), I68 (= I67), L103 (= L101), G105 (= G103), E108 (= E106), P127 (= P125), E128 (= E126), Y161 (≠ H159), F260 (= F261), K280 (≠ R281)
- binding 3-keto-decanoyl-coa: S415 (= S414), N486 (= N485), K519 (≠ P518), M520 (= M519), V525 (= V524), Y658 (≠ F644)
5mgbA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and NAD (see paper)
39% identity, 94% coverage: 15:675/703 of query aligns to 16:689/725 of 5mgbA
- active site: A66 (= A65), F71 (= F70), G81 (≠ A83), G105 (= G103), E108 (= E106), P127 (= P125), E128 (= E126), P135 (= P133), G136 (= G134), K254 (≠ E255), S415 (= S414), H436 (= H435), E448 (= E447), N486 (= N485)
- binding acetoacetyl-coenzyme a: P25 (= P24), V26 (= V25), A64 (= A63), G65 (= G64), A66 (= A65), D67 (= D66), I68 (= I67), G105 (= G103), E128 (= E126), Y161 (≠ H159)
- binding nicotinamide-adenine-dinucleotide: L307 (≠ I304), G308 (= G305), G310 (= G307), T311 (= T308), M312 (= M309), E331 (= E328), S332 (≠ A329), Q336 (≠ A333), V386 (= V385), F387 (= F386), E388 (= E387), N413 (= N412), S415 (= S414), H436 (= H435)
3zwcA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-decanoyl-coa (see paper)
39% identity, 94% coverage: 15:675/703 of query aligns to 16:689/725 of 3zwcA
- active site: A66 (= A65), F71 (= F70), G81 (≠ A83), G105 (= G103), E108 (= E106), P127 (= P125), E128 (= E126), P135 (= P133), G136 (= G134), K254 (≠ E255), S415 (= S414), H436 (= H435), E448 (= E447), N486 (= N485)
- binding (s)-3-hydroxydecanoyl-coa: V26 (= V25), A64 (= A63), G65 (= G64), A66 (= A65), D67 (= D66), I68 (= I67), G77 (≠ S79), L78 (≠ M80), L80 (≠ A82), V101 (≠ Y99), G104 (= G102), G105 (= G103), E108 (= E106), E128 (= E126), F260 (= F261)
- binding nicotinamide-adenine-dinucleotide: G308 (= G305), G310 (= G307), T311 (= T308), M312 (= M309), E331 (= E328), Q336 (≠ A333), A385 (= A384), V386 (= V385), F387 (= F386), E388 (= E387), K393 (= K392), N413 (= N412), S415 (= S414), H436 (= H435)
2x58A The crystal structure of mfe1 liganded with coa (see paper)
39% identity, 94% coverage: 15:675/703 of query aligns to 16:689/725 of 2x58A
- active site: A66 (= A65), F71 (= F70), G81 (≠ A83), G105 (= G103), E108 (= E106), P127 (= P125), E128 (= E126), P135 (= P133), G136 (= G134), K254 (≠ E255), S415 (= S414), H436 (= H435), E448 (= E447), N486 (= N485)
- binding adenosine-5'-diphosphate: G310 (= G307), T311 (= T308), M312 (= M309), E331 (= E328), S332 (≠ A329), Q336 (≠ A333), V386 (= V385), L392 (= L391)
- binding coenzyme a: V26 (= V25), A28 (= A27), A64 (= A63), A66 (= A65), D67 (= D66), I68 (= I67), E128 (= E126)
6zibAAA structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and nadh'
39% identity, 94% coverage: 15:675/703 of query aligns to 16:687/723 of 6zibAAA